MD05G1271600.v1.1

Small RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002993
Physical Location & Seq
Reverse (-)
40695276 .. 40701176
5901 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1271600.v1.1.491

Sequence Viewer

Length: 2868 bp
ATGGAAACTGAAGGAGCCGCAACTGTTGCAGTGCGAAACACTACCTTTACACCTAAGGCTATTATACACAAAAAGTTCGGTAGCACAGCCTGCTATGAAGTGGAGGAAGTGCACGAGTCTCCTCAAAATGGATGTCCAGGGTTGGCCATTATGCAGAAGGGCCCTTGCCTTTATCGCTGTACCTTGCAACTTCCAGAAGTTACTGTCGTATCTGGAACCTTTAAAAAGAAGAAGGATGCAGAGCAGTCTGCTGCTGAATTGGCCTTAGAAAAGCTAGGCATTAATCCCTCAACAAAAGTCCCTACTCTGCAGGAAGCATGGGATGAGTTAGTCTCCCGTGTCAATTTTTTATTTTCAGACGAGTTTCTTCCAGCTCTTCATCCTCTGAGTGGTCACTTCAGAGCAGCATTGCAAAGAGAAGGTGATCTTTCTGGTCAAATTCCTGCTGCAGTCATTGCCGTCTTTGATGCAACCCTTTTTAATATGTGTAAATCCATTGATCCCAAGGTGGAGTCAAATCCTTTCTTGGCTATATTGTATGTTATGAGGGCTGCTGGTAAATTATCTGGACTTCTTGCTACTTCTGAGGAAGAGCTCTGGATTAGGAGGAAGAATCCATACACTCCTGAATTAATAGAGTCATCATCTGTTGAGCAATCGGACTCCAAAGAAATATTTCCGATTGAAGCCATAAATGTACCATGTTCATTAGAGAAGAGTGTTGAAAGAGTAATCCTTAATCTCTCATCTTCTGAATATTTCCTGGATGTTATAGCAAAACAACTTGGGTTATTGAACGCTGCTGACATTCTGATCTCAAGGCCTATAGGTAAAGCTTCCTCTGAGACAAGACTGTACTTTGCTGCTCCTAAGAAATCTCTGTTGGATATCTCTTCAGATCTTCTAAATGCCAAAGGAGCTTGTAACTCTGAAGGATCTTTCAATGCAAGAGCAAGTTACTTGTCGGGCCAAGATATATATGGTGACGCTGTTTTGGCATCCATTGGATACACATGGAGGTCCAAAGACCTTTTCTACGAAGATGTATCCCTGCAATCATATCACAGGATGGTCATTGGCAAGACACCAAGTGGAATTTACAAGTTGTCCAGAGAAGCAATACTTGCAGCTGAGTTACCTTTAGCATTTACTGCCAAGGCAAAATGGAAGGGTTCGATTCCAAGGGAGATACTGTGTACATTCTGCCGCCAGCACCGGCTTGAGCCCATCTTTTCTCCTCGAAACACTCTAGAAGAATCATCCGAGTCACCAATATCACACAAGAAGTTAAAGGTGACAGACTTGGCTGGGAAAGAGACACAATATGCAAATGGCTGTGTTGTTGATGCTGGTGTAAAGGAGATAGTTGAATCCGGAGGTAGCTTTAGATGTGAAGTAAAAATAATTTCGAAGTTTCAGGATTTTATTTTAGAGTGCTCACCAAAAGAGACTTTTAAGAAGCAGAGTGATGCCATTCAGAATGTTTCTTTGAAAGTTCTGTTATGGTTGAATGCATATTTTGGGGATCCAACTATGCCTTTAGAGAGACTAAATGCTTCTGCTGATGGCCACAACATTAGATTTGATTCCCAAAACTTTATTAAAACATTTATGCTGTGCCATCATATTCATACTGTTGGGCATAAAGAGACTGAAGAAGGAAAATTATCATTTTCCAACTCTGTGAATGAACTGTTTGCTCTCCCTGGAATTGAAATTCGTTCCTTAAGTATAGAAGGACCGGATTCTGGTGTTTGTCCATCTATTGGGTCCTTAGCATGTGTAAGCTATTCTACTTCTTTGGTGACTGAAGGCAAACATACGAAAGAACTTTTGGAAAGTAGTGATGACTTTGAGTTTGAGATAGCATCTGGAGCAGTGATCCCACATCTCGAGTCAGTTATTACACAAATGACTGTTGGTCAGTCGGCTTTTTTCAGTATGGATTTGCCATCTCAAGAGTTGGTTTTAGCCGCAGCTGGCGATTCTGCTAGGATGCGTTCCTTATTGTCTTCAGAAACCTGCTATTTGGAGTACACTATAACTTTGTTGGGGGTAACCGAACCACTAGAGGATAGAATGGAGCAGGCTTTTTTCAGCCCTCCACTTTCAAAACAGCGTGTGGAATATGCAGTGCAAAGCATCAAAGAATCTTGTGCAACTACTTTGGTTGACTTTGGATGTGGCTCTGGTAGTTTGTTGGATTCTTTATTAAACTATCCAACTTCTCTGGAAAAAATTGCTGGTGTTGACATTTCACAAAAGAGTCTTGCTCGTGCGGCAAAGATACTTCATTCCAAATTAAACTCAACTTCAGATGCCATTACGTCAGCCATGAATTCAGCAATTCTTTACGATGGTTCAATTACAAATTTTGATTCCCGGTTGAGTGGATTTGATATTGGCACTTGCTTAGAGGTAATAGAGCATATGGAGGAAGATCAAGCATCCGAGTTTGGCAACGTGGTGCTAAGTTTATTTCGTCCAAGGGTTCTTATTGTCTCCACTCCAAACTACGAATACAATGTGATACTCCAGAAATCCAATTTGTCAAGCCAAGAAGATGACCCGGAGGACAAAAGTCAAGCACAATGTAAATTTCGTAACCATGACCACAAGTTCGAGTGGACGAGAGAGCAGTTCAATTGCTGGGCGACTGAATTGGCTGCAAAGCACAACTACAGTGTTGAGTTCAGTGGAGTTGGGGGTTCCGGTGATACAGAACCAGGTTTTGCTTCCCAGATTGCTGTCTTCAGACAGGAACACGTAACAAAGGAAGTTGCTTTTCCAGAGGTTTCAGATCTGGAACAACCTTTCAAAGTTATATGGGAATGGAATAGCAGCGATAGGCCAAGATCTGCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001510 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006379 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009653 GO:0009791 GO:0009892 GO:0009908 GO:0009909 GO:0009954 GO:0009987 GO:0010033 GO:0010051 GO:0010093 GO:0010305 GO:0010467 GO:0010468 GO:0010589 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016740 GO:0016741 GO:0019222 GO:0022414 GO:0031047 GO:0031050 GO:0032259 GO:0032501 GO:0032502 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035279 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048519 GO:0048580 GO:0048608 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0098542 GO:0098586 GO:0098795 GO:0099402 GO:0140098 GO:1901360 GO:1901698 GO:1901699 GO:1905392 GO:1905393 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKBP_C Methyltransf_11 Methyltransf_12 Methyltransf_31 dsrm

Protein Analysis

956

Amino Acids

105.25

Weight (kDa)

5.18

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSRM_2 PF24995 23 - 93 4.5e-13 dsRNA binding domain
Hen1_LCD PF21224 105 - 207 1.9e-42 Small RNA 2'-O-methyltransferase-like, La-motif-containing domain
Hen1_Lam_C PF18441 228 - 361 4.2e-60 Hen1 La-motif C-terminal domain
dsRBD2 PF17842 363 - 507 1e-59 Double-stranded RNA binding domain 2
Methyltransf_12 PF08242 724 - 818 1.1e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2030
AccB7I CCANNNNNTGG 1 cut(s) 1766
AccIII TCCGGA 1 cut(s) 1373
AciI CCGC 4 cut(s) 18, 1207, 1974, 2279
AclWI GGATC 5 cut(s) 494, 943, 1520, 1533, 1876
AcoI YGGCCR 2 cut(s) 144, 1567
AcsI RAATTY 6 cut(s) 438, 1095, 1716, 2338, 2371, 2597
AcuI CTGAAG 9 cut(s) 30, 382, 879, 951, 1674, 1830, 1998, 2298, 2737
AdeI CACNNNGTG 1 cut(s) 1091
AfaI GTAC 5 cut(s) 181, 699, 857, 1198, 2036
AfiI CCNNNNNNNGG 4 cut(s) 128, 389, 1748, 1766
AflII CTTAAG 1 cut(s) 1726
AflIII ACRYGT 1 cut(s) 2764
AhdI GACNNNNNGTC 1 cut(s) 1920
AjnI CCWGG 4 cut(s) 136, 762, 1705, 2725
AjuI GAANNNNNNNTTGG 6 cut(s) 866, 898, 1016, 1048, 1817, 1849
AluBI AGCT 9 cut(s) 274, 374, 595, 836, 920, 1130, 1383, 1788, 1979
AluI AGCT 9 cut(s) 274, 374, 595, 836, 920, 1130, 1383, 1788, 1979
Alw21I GWGCWC 3 cut(s) 114, 597, 1439
Alw26I GTCTC 8 cut(s) 123, 337, 839, 1310, 1442, 1540, 1643, 2506
Alw44I GTGCAC 1 cut(s) 110
AlwI GGATC 5 cut(s) 494, 943, 1520, 1533, 1876
Ama87I CYCGRG 1 cut(s) 1892
Aor13HI TCCGGA 1 cut(s) 1373
AoxI GGCC 7 cut(s) 144, 160, 261, 821, 967, 1567, 2849
ApaI GGGCCC 1 cut(s) 164
ApaLI GTGCAC 1 cut(s) 110
ApoI RAATTY 6 cut(s) 438, 1095, 1716, 2338, 2371, 2597
ArsI GACNNNNNNTTYG 6 cut(s) 118, 150, 1841, 1873, 2603, 2635
AseI ATTAAT 2 cut(s) 282, 632
Asp700I GAANNNNTTC 1 cut(s) 675
AspS9I GGNCC 6 cut(s) 160, 161, 967, 1020, 1739, 1770
AsuC2I CCSGG 2 cut(s) 2383, 2570
AsuHPI GGTGA 7 cut(s) 434, 995, 1260, 1306, 1431, 1816, 2726
AsuII TTCGAA 1 cut(s) 1409
AvaI CYCGRG 1 cut(s) 1892
AvaII GGWCC 3 cut(s) 1020, 1739, 1770
AxyI CCTNAGG 1 cut(s) 54
BaeGI GKGCMC 2 cut(s) 114, 164
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BalI TGGCCA 2 cut(s) 146, 1569
BamHI GGATCC 1 cut(s) 1525
BanII GRGCYC 3 cut(s) 164, 597, 1227
BarI GAAGNNNNNNTAC 2 cut(s) 602, 634
BauI CACGAG 2 cut(s) 113, 2274
BbsI GAAGAC 2 cut(s) 2004, 2743
Bbv12I GWGCWC 3 cut(s) 114, 597, 1439
BccI CCATC 7 cut(s) 1063, 1235, 1559, 1629, 1768, 1960, 2351
BceAI ACGGC 1 cut(s) 443
BciT130I CCWGG 4 cut(s) 138, 764, 1707, 2727
BciVI GTATCC 2 cut(s) 1001, 1057
BcnI CCSGG 2 cut(s) 2383, 2570
BcoDI GTCTC 8 cut(s) 123, 337, 839, 1310, 1442, 1540, 1643, 2506
BfaI CTAG 4 cut(s) 275, 1250, 1992, 2069
BfmI CTRYAG 4 cut(s) 308, 447, 825, 2680
BfrI CTTAAG 1 cut(s) 1726
BfuAI ACCTGC 1 cut(s) 2030
BfuI GTATCC 2 cut(s) 1001, 1057
BglII AGATCT 3 cut(s) 898, 2800, 2855
Bme1390I CCNGG 6 cut(s) 138, 764, 1707, 2383, 2570, 2727
Bme18I GGWCC 3 cut(s) 1020, 1739, 1770
BmeRI GACNNNNNGTC 1 cut(s) 1920
BmeT110I CYCGRG 1 cut(s) 1892
BmgT120I GGNCC 6 cut(s) 160, 161, 967, 1020, 1739, 1770
BmiI GGNNCC 6 cut(s) 16, 162, 217, 1527, 1771, 2710
BmrFI CCNGG 6 cut(s) 138, 764, 1707, 2383, 2570, 2727
BoxI GACNNNNGTC 1 cut(s) 2580
BpiI GAAGAC 2 cut(s) 2004, 2743
BplI GAGNNNNNCTC 4 cut(s) 317, 349, 2257, 2289
BpmI CTGGAG 2 cut(s) 1893, 2519
Bpu10I CCTNAGC 1 cut(s) 1774
Bpu14I TTCGAA 1 cut(s) 1409
BpuEI CTTGAG 3 cut(s) 802, 1241, 1941
BpuMI CCSGG 2 cut(s) 2383, 2570
BsaAI YACGTR 1 cut(s) 2767
BsaJI CCNNGG 6 cut(s) 137, 504, 1155, 1181, 1705, 2486
BsaWI WCCGGW 3 cut(s) 1373, 1741, 2711
BsaXI ACNNNNNCTCC 2 cut(s) 1178, 1208
Bsc4I CCNNNNNNNGG 4 cut(s) 128, 389, 1748, 1766
Bse118I RCCGGY 1 cut(s) 1215
Bse21I CCTNAGG 1 cut(s) 54
Bse3DI GCAATG 2 cut(s) 407, 453
BseAI TCCGGA 1 cut(s) 1373
BseBI CCWGG 4 cut(s) 138, 764, 1707, 2727
BseDI CCNNGG 6 cut(s) 137, 504, 1155, 1181, 1705, 2486
BseLI CCNNNNNNNGG 4 cut(s) 128, 389, 1748, 1766
BseMI GCAATG 2 cut(s) 407, 453
BseMII CTCAG 4 cut(s) 377, 576, 834, 1122
BseRI GAGGAG 2 cut(s) 111, 1227
BseSI GKGCMC 2 cut(s) 114, 164
BseYI CCCAGC 2 cut(s) 1307, 2649
BshFI GGCC 7 cut(s) 146, 162, 263, 823, 969, 1569, 2851
BsiHKAI GWGCWC 3 cut(s) 114, 597, 1439
BsiHKCI CYCGRG 1 cut(s) 1892
BsiSI CCGG 6 cut(s) 1216, 1374, 1742, 2383, 2570, 2712
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 4 cut(s) 128, 389, 1748, 1766
BsmAI GTCTC 8 cut(s) 123, 337, 839, 1310, 1442, 1540, 1643, 2506
BsmFI GGGAC 1 cut(s) 284
BsmI GAATGC 1 cut(s) 1516
BsnI GGCC 7 cut(s) 146, 162, 263, 823, 969, 1569, 2851
BsoBI CYCGRG 1 cut(s) 1892
Bsp119I TTCGAA 1 cut(s) 1409
Bsp120I GGGCCC 1 cut(s) 160
Bsp1286I GDGCHC 5 cut(s) 114, 164, 597, 1227, 1439
Bsp13I TCCGGA 1 cut(s) 1373
Bsp1407I TGTACA 1 cut(s) 1196
BspACI CCGC 4 cut(s) 18, 1207, 1974, 2279
BspANI GGCC 7 cut(s) 146, 162, 263, 823, 969, 1569, 2851
BspCNI CTCAG 4 cut(s) 378, 577, 835, 1123
BspEI TCCGGA 1 cut(s) 1373
BspLI GGNNCC 6 cut(s) 16, 162, 217, 1527, 1771, 2710
BspMAI CTGCAG 2 cut(s) 312, 451
BspMI ACCTGC 1 cut(s) 2030
BspPI GGATC 5 cut(s) 494, 943, 1520, 1533, 1876
BspQI GCTCTTC 2 cut(s) 381, 585
BspT104I TTCGAA 1 cut(s) 1409
BspTI CTTAAG 1 cut(s) 1726
BsrDI GCAATG 2 cut(s) 407, 453
BsrFI RCCGGY 1 cut(s) 1215
BsrGI TGTACA 1 cut(s) 1196
BssAI RCCGGY 1 cut(s) 1215
BssECI CCNNGG 6 cut(s) 137, 504, 1155, 1181, 1705, 2486
BssSI CACGAG 2 cut(s) 113, 2274
BssT1I CCWWGG 4 cut(s) 504, 1155, 1181, 2486
Bst2BI CACGAG 2 cut(s) 113, 2274
Bst2UI CCWGG 4 cut(s) 138, 764, 1707, 2727
Bst4CI ACNGT 8 cut(s) 25, 205, 855, 1194, 1636, 1695, 1918, 2684
Bst6I CTCTTC 4 cut(s) 381, 585, 710, 898
BstAFI CTTAAG 1 cut(s) 1726
BstAPI GCANNNNNTGC 5 cut(s) 26, 90, 455, 1124, 1151
BstAUI TGTACA 1 cut(s) 1196
BstBAI YACGTR 1 cut(s) 2767
BstBI TTCGAA 1 cut(s) 1409
BstC8I GCNNGC 4 cut(s) 91, 1211, 1981, 2088
BstEII GGTNACC 1 cut(s) 2056
BstMAI GTCTC 8 cut(s) 123, 337, 839, 1310, 1442, 1540, 1643, 2506
BstNI CCWGG 4 cut(s) 138, 764, 1707, 2727
BstNSI RCATGY 1 cut(s) 1782
BstPAI GACNNNNGTC 1 cut(s) 2580
BstPI GGTNACC 1 cut(s) 2056
BstSCI CCNGG 6 cut(s) 136, 762, 1705, 2381, 2568, 2725
BstSFI CTRYAG 4 cut(s) 308, 447, 825, 2680
BstSLI GKGCMC 2 cut(s) 114, 164
BstV2I GAAGAC 2 cut(s) 2004, 2743
BstX2I RGATCY 5 cut(s) 898, 935, 1525, 2800, 2855
BstYI RGATCY 5 cut(s) 898, 935, 1525, 2800, 2855
Bsu36I CCTNAGG 1 cut(s) 54
BsuI GTATCC 2 cut(s) 1001, 1057
BsuRI GGCC 7 cut(s) 146, 162, 263, 823, 969, 1569, 2851
BtsI GCAGTG 3 cut(s) 36, 1884, 2139
BtsIMutI CAGTG 5 cut(s) 36, 1884, 2139, 2689, 2701
BveI ACCTGC 1 cut(s) 2030
Cac8I GCNNGC 4 cut(s) 91, 1211, 1981, 2088
Cfr10I RCCGGY 1 cut(s) 1215
Cfr13I GGNCC 6 cut(s) 160, 161, 967, 1020, 1739, 1770
CseI GACGC 1 cut(s) 995
CsiI ACCWGGT 1 cut(s) 2725
Csp6I GTAC 5 cut(s) 180, 698, 856, 1197, 2035
CviAII CATG 6 cut(s) 318, 702, 1014, 1779, 2335, 2609
CviQI GTAC 5 cut(s) 180, 698, 856, 1197, 2035
DraI TTTAAA 1 cut(s) 223
DraIII CACNNNGTG 1 cut(s) 1091
DriI GACNNNNNGTC 1 cut(s) 1920
EaeI YGGCCR 2 cut(s) 144, 1567
Eam1104I CTCTTC 4 cut(s) 381, 585, 710, 898
Eam1105I GACNNNNNGTC 1 cut(s) 1920
EarI CTCTTC 4 cut(s) 381, 585, 710, 898
Ecl136II GAGCTC 1 cut(s) 595
Eco130I CCWWGG 4 cut(s) 504, 1155, 1181, 2486
Eco147I AGGCCT 1 cut(s) 823
Eco24I GRGCYC 3 cut(s) 164, 597, 1227
Eco32I GATATC 1 cut(s) 889
Eco47I GGWCC 3 cut(s) 1020, 1739, 1770
Eco53kI GAGCTC 1 cut(s) 595
Eco57I CTGAAG 9 cut(s) 30, 382, 879, 951, 1674, 1830, 1998, 2298, 2737
Eco81I CCTNAGG 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 1892
Eco91I GGTNACC 1 cut(s) 2056
EcoICRI GAGCTC 1 cut(s) 595
EcoO109I RGGNCCY 3 cut(s) 160, 161, 1770
EcoO65I GGTNACC 1 cut(s) 2056
EcoRI GAATTC 1 cut(s) 2338
EcoRII CCWGG 4 cut(s) 136, 762, 1705, 2725
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 4 cut(s) 504, 1155, 1181, 2486
EcoT22I ATGCAT 1 cut(s) 1516
EcoT38I GRGCYC 3 cut(s) 164, 597, 1227
ErhI CCWWGG 4 cut(s) 504, 1155, 1181, 2486
FaeI CATG 6 cut(s) 321, 705, 1017, 1782, 2338, 2612
FalI AAGNNNNNCTT 4 cut(s) 411, 443, 1107, 1139
FaqI GGGAC 1 cut(s) 284
FatI CATG 6 cut(s) 317, 701, 1013, 1778, 2334, 2608
FauNDI CATATG 1 cut(s) 2430
FriOI GRGCYC 3 cut(s) 164, 597, 1227
FspBI CTAG 4 cut(s) 275, 1250, 1992, 2069
GsaI CCCAGC 2 cut(s) 1311, 2653
GsuI CTGGAG 2 cut(s) 1893, 2519
HaeIII GGCC 7 cut(s) 146, 162, 263, 823, 969, 1569, 2851
HapII CCGG 6 cut(s) 1216, 1374, 1742, 2383, 2570, 2712
HgaI GACGC 1 cut(s) 995
Hin1II CATG 6 cut(s) 321, 705, 1017, 1782, 2338, 2612
HincII GTYRAC 2 cut(s) 2173, 2251
HindII GTYRAC 2 cut(s) 2173, 2251
HindIII AAGCTT 1 cut(s) 834
HpaII CCGG 6 cut(s) 1216, 1374, 1742, 2383, 2570, 2712
HphI GGTGA 7 cut(s) 434, 995, 1260, 1306, 1431, 1816, 2726
Hpy166II GTNNAC 6 cut(s) 112, 1197, 2037, 2173, 2251, 2628
Hpy8I GTNNAC 6 cut(s) 112, 1197, 2037, 2173, 2251, 2628
HpyAV CCTTC 9 cut(s) 5, 151, 226, 413, 926, 1162, 1652, 1730, 1805
HpyCH4III ACNGT 8 cut(s) 25, 205, 855, 1194, 1636, 1695, 1918, 2684
HpyCH4IV ACGT 3 cut(s) 2327, 2463, 2766
HpySE526I ACGT 3 cut(s) 2327, 2463, 2766
Hsp92II CATG 6 cut(s) 321, 705, 1017, 1782, 2338, 2612
Kpn2I TCCGGA 1 cut(s) 1373
LguI GCTCTTC 2 cut(s) 381, 585
LmnI GCTCC 5 cut(s) 14, 871, 917, 1874, 2083
MabI ACCWGGT 1 cut(s) 2725
MaeI CTAG 4 cut(s) 275, 1250, 1992, 2069
MaeII ACGT 3 cut(s) 2327, 2463, 2766
MfeI CAATTG 1 cut(s) 2644
MflI RGATCY 5 cut(s) 898, 935, 1525, 2800, 2855
MhlI GDGCHC 5 cut(s) 114, 164, 597, 1227, 1439
MlsI TGGCCA 2 cut(s) 146, 1569
MluNI TGGCCA 2 cut(s) 146, 1569
MlyI GAGTC 7 cut(s) 125, 521, 647, 656, 1274, 1904, 2275
MmeI TCCRAC 5 cut(s) 864, 1553, 1701, 2181, 2246
Mox20I TGGCCA 2 cut(s) 146, 1569
Mph1103I ATGCAT 1 cut(s) 1516
MroI TCCGGA 1 cut(s) 1373
MroXI GAANNNNTTC 1 cut(s) 675
MscI TGGCCA 2 cut(s) 146, 1569
Msp20I TGGCCA 2 cut(s) 146, 1569
MspA1I CMGCKG 2 cut(s) 1130, 1979
MspCI CTTAAG 1 cut(s) 1726
MspI CCGG 6 cut(s) 1216, 1374, 1742, 2383, 2570, 2712
MspR9I CCNGG 6 cut(s) 138, 764, 1707, 2383, 2570, 2727
MunI CAATTG 1 cut(s) 2644
Mva1269I GAATGC 1 cut(s) 1516
MvaI CCWGG 4 cut(s) 138, 764, 1707, 2727
NciI CCSGG 2 cut(s) 2383, 2570
NdeI CATATG 1 cut(s) 2430
NlaIII CATG 6 cut(s) 321, 705, 1017, 1782, 2338, 2612
NlaIV GGNNCC 6 cut(s) 16, 162, 217, 1527, 1771, 2710
NmuCI GTSAC 5 cut(s) 392, 983, 1266, 1294, 1804
NsiI ATGCAT 1 cut(s) 1516
NspI RCATGY 1 cut(s) 1782
NspV TTCGAA 1 cut(s) 1409
PaeR7I CTCGAG 1 cut(s) 1892
PceI AGGCCT 1 cut(s) 823
PciSI GCTCTTC 2 cut(s) 381, 585
PctI GAATGC 1 cut(s) 1516
PdmI GAANNNNTTC 1 cut(s) 675
PflMI CCANNNNNTGG 1 cut(s) 1766
PfoI TCCNGGA 1 cut(s) 762
PleI GAGTC 7 cut(s) 124, 520, 646, 656, 1273, 1903, 2274
PpsI GAGTC 7 cut(s) 124, 520, 646, 656, 1273, 1903, 2274
Ppu21I YACGTR 1 cut(s) 2767
PpuMI RGGWCCY 1 cut(s) 1770
PshAI GACNNNNGTC 1 cut(s) 2580
PshBI ATTAAT 2 cut(s) 282, 632
Psp124BI GAGCTC 1 cut(s) 597
Psp5II RGGWCCY 1 cut(s) 1770
Psp6I CCWGG 4 cut(s) 136, 762, 1705, 2725
PspEI GGTNACC 1 cut(s) 2056
PspFI CCCAGC 2 cut(s) 1307, 2649
PspGI CCWGG 4 cut(s) 136, 762, 1705, 2725
PspN4I GGNNCC 6 cut(s) 16, 162, 217, 1527, 1771, 2710
PspOMI GGGCCC 1 cut(s) 160
PspPI GGNCC 6 cut(s) 160, 161, 967, 1020, 1739, 1770
PspPPI RGGWCCY 1 cut(s) 1770
PstI CTGCAG 2 cut(s) 312, 451
PsuI RGATCY 5 cut(s) 898, 935, 1525, 2800, 2855
PvuII CAGCTG 2 cut(s) 1130, 1979
RsaI GTAC 5 cut(s) 181, 699, 857, 1198, 2036
RsaNI GTAC 5 cut(s) 180, 698, 856, 1197, 2035
SacI GAGCTC 1 cut(s) 597
SapI GCTCTTC 2 cut(s) 381, 585
Sau96I GGNCC 6 cut(s) 160, 161, 967, 1020, 1739, 1770
SchI GAGTC 7 cut(s) 125, 521, 647, 656, 1274, 1904, 2275
ScrFI CCNGG 6 cut(s) 138, 764, 1707, 2383, 2570, 2727
SduI GDGCHC 5 cut(s) 114, 164, 597, 1227, 1439
SexAI ACCWGGT 1 cut(s) 2725
SfcI CTRYAG 4 cut(s) 308, 447, 825, 2680
Sfr274I CTCGAG 1 cut(s) 1892
SfuI TTCGAA 1 cut(s) 1409
SinI GGWCC 3 cut(s) 1020, 1739, 1770
SlaI CTCGAG 1 cut(s) 1892
SmlI CTYRAG 5 cut(s) 817, 1220, 1726, 1892, 1956
SmoI CTYRAG 5 cut(s) 817, 1220, 1726, 1892, 1956
SseBI AGGCCT 1 cut(s) 823
SsiI CCGC 4 cut(s) 18, 1207, 1974, 2279
SspI AATATT 2 cut(s) 675, 758
SspMI CTAG 4 cut(s) 275, 1250, 1992, 2069
SstI GAGCTC 1 cut(s) 597
StuI AGGCCT 1 cut(s) 823
StyD4I CCNGG 6 cut(s) 136, 762, 1705, 2381, 2568, 2725
StyI CCWWGG 4 cut(s) 504, 1155, 1181, 2486
TaaI ACNGT 8 cut(s) 25, 205, 855, 1194, 1636, 1695, 1918, 2684
TaiI ACGT 3 cut(s) 2330, 2466, 2769
TaqI TCGA 5 cut(s) 1175, 1240, 1409, 1893, 2622
TatI WGTACW 3 cut(s) 855, 1196, 2034
TauI GCSGC 4 cut(s) 20, 1209, 1976, 2282
TscAI CASTG 5 cut(s) 36, 1884, 2139, 2689, 2701
TseFI GTSAC 5 cut(s) 392, 983, 1266, 1294, 1804
Tsp45I GTSAC 5 cut(s) 392, 983, 1266, 1294, 1804
TspDTI ATGAA 7 cut(s) 111, 368, 696, 1619, 1704, 2282, 2351
TspRI CASTG 5 cut(s) 36, 1884, 2139, 2689, 2701
Van91I CCANNNNNTGG 1 cut(s) 1766
Vha464I CTTAAG 1 cut(s) 1726
VneI GTGCAC 1 cut(s) 110
VpaK11BI GGWCC 3 cut(s) 1020, 1739, 1770
VspI ATTAAT 2 cut(s) 282, 632
XapI RAATTY 6 cut(s) 438, 1095, 1716, 2338, 2371, 2597
XbaI TCTAGA 1 cut(s) 1249
XceI RCATGY 1 cut(s) 1782
XcmI CCANNNNNNNNNTGG 1 cut(s) 977
XhoI CTCGAG 1 cut(s) 1892
XmnI GAANNNNTTC 1 cut(s) 675
XspI CTAG 4 cut(s) 275, 1250, 1992, 2069
Zsp2I ATGCAT 1 cut(s) 1516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.