RLG00000032251

Small RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002993
Physical Location & Seq
Forward (+)
12601221 .. 12611027
9807 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032251

Sequence Viewer

Length: 4341 bp
ATGGAAACTGAAGGAGCCTCATCTGTCACAGTTCGAAAGACTAGCTGTACACCTAAGGCTATTATACACCAAAAGTTTGGTACCAATGCCTGCTATAAACTTGAGGAAGTGCATGAGTCTGCTGAAAATGGATGTCCAGGATTAGCCATTGTGCAGAAAGGCCCTTGCCTTTACCGCTGTACCTTGCAGCTTCCAGAAATTACTGTTGTATCTGGAATCTTTAAAAAGAAGAAGGATGCTGAGCAATCTGCTGCTGAATTGGCCTTAGAAAAGCTGCGCATCACTCCCGCCACAAAAAGCTCCTCTCTTTCAGAAACATGGGATGGTTTAGTCTCTCGGGTCAAATATTTATTTTCAGAAGAGTTCCTTTCATCTCTTCATCCGCTGAGTGGTCATTTGAGAGCAGCTTTGCAAAGAGAAGGTCATCTTGCTGGCTTGATCCCTGCTTGTGCTATAGCCGTCTTTGATCCAACACTAAGTAATCTGTGTAAATCCATTGATTCTAAGGTGGAAGCAAATCCCTTCTTGATAATATCATATGTTATGAGAGCTGCTGCTAAATTATCTGGATTTATTGCTAGTTCAGAGCGAGAGCTCTGGATTAGGAGGCAAAATCCATACCCTCCAGAAATTATAGAGTTATCATCTATCCAGCAAAGTCCAAACAATTCTGAAATTATAGTCTCACCATCTAACCGGCAATCAGGCTCTCCTGAAATCCTTATGGTTGAAGCCATAAATATACCGTGTTCACTAGAGAAGACTGTTGAAAGAGTAATTCTTAATCTAACATCTTCTGGTTATTTCCTGGATGTTATTGCAAAGCAACTTGGGTTGTTGGAAGCTGCTGACCTTATGATCTCAAGGCCTGTAGGTAAGGCTTCCTCTGAGACAAGGCTGCACTTTGCTGCTCCAAAGCAATATCTGTTGGATATTTCTTCAGATCTTCTTCATGCCAAAGAAGTTCGTAGTTTTGAAGGATCTTTGAATGCAAGAGCAAGTTACTTGTCTGGTCAAGATATCTATGGTGATGCTATTTTGGCAACCATTGGCTACACATGGAGGTCCAAAGACCTTTTCTATGAAGATGTATCTGTGAAATCATATTACAGGATGCTTATTGGCAAGACACCAAGTGGACTTTACAGGCTGTCCAGAGGGGCAATACTTGCAGCTGAGTTACCTTTGGCATTTACTACAAATGCAAAATGGAAGGGTTCCTTACCAAGGGAGATGCTTTGTACATTCTGTCGTCAGCATCGGCTTTCTCAGCCTATCTTCTGTACTTTAAGCAGTCTAGAAGAATCATCTGACTCCTCACAATCACACAAGAAGTTAAGGGCGACAGACTTGGTTGCGAAACACACACAACATGCAAATGGTTGTGTTGTTACTGCTGGTTCAAAGGAAACAGTGGAGTCCGGAGGTAGCTTTAGATGTGAAATAAAAGTATATTCTAAGTTTCAGGATTTGATTTTAGAATGCTCACCAAAAGAGTCTTTTAAGAAGCAGAGTGATTCTGTCCAGAATGCTTCTTTGAAAGTTCTCTTATGGTTAGATATATACTTTAGGGATCCAAATGTGCCTTTCGAGAGATTAAAGGCTTCTGCTGATGGACTTGACATTAGATTTGAACCAAAAAATTTTTTTGAAGCATTTATGCTTTGTCAATCTCTTCATAATGTCGGTCATAATGAGATTGAAGAAGGCAAATTAGTATATCCCAACTCTGTGAATGTGCCAAATGCTCTATCTGGACACGGACTTCGTTCTCTGAATATAGAAGGCCCTGATTCTGGTATTTCTCCATCTAATGGGTCCTTATCATGTGTAAGTTATTCTGCATCTCTAGTGACTGAAGAGCATATAAAAGAACAACTGGAAAGTAGTGATGACTTTGAGTTTGAGATAGCTTCTGGAGCTGTGAATCTGCATCTTGAATCAGTTCTCATTCAGATGTCGGTCGGTCAGTCTGCTTGTTTCATTGTGGATCTACCCCCTCGAGAGTTGATTTTTGCTGCAGCTGATGATTCTGCAAGGATGCTGTCCTTATTGTCTTCAAAAACCTGCTGCTTGGAGTACACTATAAGGTTGTTGCAGGTAACAGAACCCTTGGAGGACAGAATGGAGCAGGCCTTGTTCAGCCCTCCACTTTCAAAACAACGAGTGGAATATGCAGTGCAACGCATCAAAGAATATTGTGCTACTACTTTGGTTGACTTTGGATGTGGCTCTGGTAGCTTGTTGGATTCCTTATTAAATTATCCAACTTCTCTGGAAAAAATTGCTGGTGTTGATCTTTCACACAAGAGTCTTACCCGTGCGGCAAAGATACTTAATTCCAAATTAAACTCGACTTCAGATGCTGATACCTCATGTACACTCCTAAAATCTGCAGTTCTTTATGATGGTTCGGTTACAGATCATGATTCTCGATTGTGTGGGTTTGATATCGGCACTTGCTTAGAGGTAATCGAGCATATGGAGGAAGATCAAGCACATCTCTTTGGCAATGTGGCATTGAGTTATTTTCGTCCTAAAATTCTCATTGTCTCGACGCCAAACTATGAATATAATGTCATACTCCAGAAGTCTACTTTATCAACCACAGAAGATGATGTTGATGAAAGAAATGAATCACAACCTTGTAAGTTTCGTAACCATGACCACAAGTTTGAGTGGACGAGAGCGCAGTTCAATTGCTGGGCAACTGAATTGGCCACGAGGCACGACTACAGTGTTGAGTTCAGTGGGGTTGGTGGATCCGGTGATGAACCAGGTTTTGCTTCCCAGATTGCTGTCTTCAGACGGAGAGCCATACCTCAGGAAGGTTATCCTGAAGAACTCTCGGATTCGGAACATCGTTACCAAGTCATATGGGAATGGACTAGGAGCGATAGACCTCATGACACCACTTATGACTGCACTGCTCAGTTGAAGCCCAAAACCAAGTTTAGGGATGTACTAAAAGATGCTGCGGAAATCAGAGGCGTGTTAAATTCCAGTCGTTCCACAAAGTTGAAACCATTCCTCACCAGGTATGTGCTAACCTCTGAAACTACCCCAGGCTTTGACAAATGGTGGCATAGTCAAACCAAAGATCGGTTTTCGGTAGAACATGATGGGCCCTTCGCTATCTTATATATGCATTGTCCATTCCGGGATAGCCATCATCTAGAAGTTCCAGGCTTTCCCCCTGCTGATGGAGAAAGGGAAGAAGGTGAAGAGGGAGAAGCTAAAGTTGAAAGCCCTCTCCCTACTGACAAGAATCGAAAGAGGAAAGGCAACACAAATGCTCCAAAATCAGCTAGGCAGAAGACTACTCAAGTCACAGTATCTGGAATGAAGACAAATGGATCCAATAATGAAGAAGCAGAGGAAATTGATGGCCGTCATACCCTGAATGTTGATGGCTTGTCTGGAGCTCATATTAAGGAATCCACTGTTGACCACCAAAATGCTGTTGTTAAGCCAAAACATATAACTTCAACTTCTAAAAATACCTTCAGATCTATGAAAAAGCTGAGGTCATATTTTTCAAGTGACACCTCCGAAAACGCTGATAACATCGAAACTGAAGAACCTGAACAGACTGAAGGGCCATTGATGGGAACTGGGGAGTTAGGAAGTCAACAAATGTATAGCGATGCTGCTGCCTTCGAAACTGCCCCCTTACTTACCAGTATGCACCCAGCTCAAAGTGAGCCAACCACGCCAGTTCAAGGATTACTAGACAGCAATGTGAGGACCCTCTCAAGAGAGGAAAAGTTGAAAAAGTTTGAAAATATTGAAAATAGTGTGGCAAACTCATCTGTTGAAGCTTCCAAAGCTGAAGATGATCATGCTTATTCCGTTATTGACGACGTGGTGGTTCGAGGCACGAGGGCAATTTTTCATGATGGCCAGGTCCCAGCAGACCTAAGAGAGGCATTAAATTATGTTCGAGGCAGAAGTCCGTTAACCCATGTTTTGATTGATGCTCTTGATGAGTATTTTAAGCAGCTTAATTGCGCCCAGACGAAAATGACTATGAATTTCAGTAAGTTGACGTTGCTCGAAGATGAAGTTCGACGAGATAAGGACATCAAGCTGGATAAAGCATCAAATGAAGGAAAGCAGTTGCAAATTGCAGCAATACAAAGAGCGGAGGAAATTTCTCGACTTGATGAAGAAATAAAGGTACTTAAAGCAGAGAGAGAGGAGGAAGAGCGTATGGTGGCACTAGATAAAAATGTTATTGATGAGAAATTTGCACTCATTAGTTCCTTTGAACTTGAAATGTCCAAGCTCCCCGATCGTGGTTCTGAAATGATTGCCTGCCAAATAAAGGATAAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001510 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006379 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009653 GO:0009791 GO:0009892 GO:0009908 GO:0009909 GO:0009954 GO:0009987 GO:0010033 GO:0010051 GO:0010093 GO:0010305 GO:0010467 GO:0010468 GO:0010589 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016740 GO:0016741 GO:0019222 GO:0022414 GO:0031047 GO:0031050 GO:0032259 GO:0032501 GO:0032502 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035279 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048519 GO:0048580 GO:0048608 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0098542 GO:0098586 GO:0098795 GO:0099402 GO:0140098 GO:1901360 GO:1901698 GO:1901699 GO:1905392 GO:1905393 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKBP_C Methyltransf_11 Methyltransf_12 Methyltransf_31 dsrm

Protein Analysis

1447

Amino Acids

160.97

Weight (kDa)

5.54

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSRM_2 PF24995 23 - 93 2.9e-12 dsRNA binding domain
Hen1_LCD PF21224 105 - 208 1.4e-41 Small RNA 2'-O-methyltransferase-like, La-motif-containing domain
Hen1_Lam_C PF18441 242 - 376 4.4e-59 Hen1 La-motif C-terminal domain
dsRBD2 PF17842 378 - 523 2.2e-59 Double-stranded RNA binding domain 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 278
Acc36I ACCTGC 2 cut(s) 2075, 2089
Acc65I GGTACC 1 cut(s) 80
AccB1I GGYRCC 1 cut(s) 80
AccB7I CCANNNNNTGG 1 cut(s) 1814
AccBSI CCGCTC 1 cut(s) 4145
AccI GTMKAC 1 cut(s) 2594
AccIII TCCGGA 1 cut(s) 1421
AciI CCGC 6 cut(s) 175, 288, 383, 2324, 2978, 4145
AcoI YGGCCR 3 cut(s) 2718, 3388, 3901
AcsI RAATTY 6 cut(s) 1642, 2541, 2998, 4033, 4152, 4247
AcyI GRCGYC 1 cut(s) 2558
AdeI CACNNNGTG 1 cut(s) 1136
AfaI GTAC 9 cut(s) 49, 82, 181, 1243, 1285, 2081, 2380, 2964, 4182
AjiI CACGTC 1 cut(s) 3865
AjnI CCWGG 7 cut(s) 136, 807, 2776, 3035, 3064, 3184, 3903
AjuI GAANNNNNNNTTGG 2 cut(s) 1061, 1093
Alw21I GWGCWC 2 cut(s) 597, 3427
Alw26I GTCTC 4 cut(s) 337, 688, 884, 2557
AlwNI CAGNNNCTG 2 cut(s) 2366, 3338
Ama87I CYCGRG 2 cut(s) 336, 2001
Aor13HI TCCGGA 1 cut(s) 1421
ApaI GGGCCC 1 cut(s) 3129
ApoI RAATTY 6 cut(s) 1642, 2541, 2998, 4033, 4152, 4247
Asp700I GAANNNNTTC 2 cut(s) 1944, 3026
Asp718I GGTACC 1 cut(s) 80
AspLEI GCGC 3 cut(s) 279, 2692, 4013
AspS9I GGNCC 9 cut(s) 161, 1065, 1787, 1818, 3125, 3126, 3599, 3747, 3907
AsuC2I CCSGG 1 cut(s) 3161
AsuHPI GGTGA 6 cut(s) 678, 1040, 1479, 2780, 3025, 3233
AsuII TTCGAA 2 cut(s) 34, 3660
AvaI CYCGRG 2 cut(s) 336, 2001
AvaII GGWCC 4 cut(s) 1065, 1818, 3747, 3907
AxyI CCTNAGG 2 cut(s) 54, 2823
BaeGI GKGCMC 1 cut(s) 3129
BaeI ACNNNNGTAYC 4 cut(s) 163, 192, 196, 225
BalI TGGCCA 2 cut(s) 2720, 3903
BamHI GGATCC 3 cut(s) 1573, 2762, 3356
BanI GGYRCC 1 cut(s) 80
BanII GRGCYC 3 cut(s) 597, 3129, 3427
BauI CACGAG 2 cut(s) 2722, 3880
BbsI GAAGAC 5 cut(s) 767, 2049, 2794, 3323, 3353
Bbv12I GWGCWC 2 cut(s) 597, 3427
BbvCI CCTCAGC 1 cut(s) 3524
BceAI ACGGC 2 cut(s) 443, 3375
BcgI CGANNNNNNTGC 2 cut(s) 3635, 3669
BciT130I CCWGG 7 cut(s) 138, 809, 2778, 3037, 3066, 3186, 3905
BclI TGATCA 1 cut(s) 3838
BcnI CCSGG 1 cut(s) 3161
BcoDI GTCTC 4 cut(s) 337, 688, 884, 2557
BfmI CTRYAG 5 cut(s) 453, 870, 2019, 2394, 2734
BfuAI ACCTGC 2 cut(s) 2075, 2089
BglII AGATCT 2 cut(s) 943, 3509
BlpI GCTNAGC 1 cut(s) 240
Bme1390I CCNGG 8 cut(s) 138, 809, 2778, 3037, 3066, 3161, 3186, 3905
Bme18I GGWCC 4 cut(s) 1065, 1818, 3747, 3907
BmeT110I CYCGRG 2 cut(s) 336, 2001
BmgBI CACGTC 1 cut(s) 3865
BmgT120I GGNCC 9 cut(s) 161, 1065, 1787, 1818, 3125, 3126, 3599, 3747, 3907
BmrFI CCNGG 8 cut(s) 138, 809, 2778, 3037, 3066, 3161, 3186, 3905
BmrI ACTGGG 1 cut(s) 3624
BmuI ACTGGG 1 cut(s) 3624
BpiI GAAGAC 5 cut(s) 767, 2049, 2794, 3323, 3353
BpmI CTGGAG 4 cut(s) 609, 1938, 2570, 3441
Bpu10I CCTNAGC 1 cut(s) 3524
Bpu1102I GCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 2 cut(s) 34, 3660
BpuEI CTTGAG 4 cut(s) 122, 847, 3309, 3739
BpuMI CCSGG 1 cut(s) 3161
BsaBI GATNNNNATC 1 cut(s) 3168
BsaHI GRCGYC 1 cut(s) 2558
BsaJI CCNNGG 3 cut(s) 1226, 2112, 3064
BsaWI WCCGGW 2 cut(s) 1421, 2765
BsaXI ACNNNNNCTCC 2 cut(s) 3280, 3310
Bse118I RCCGGY 1 cut(s) 696
Bse1I ACTGG 5 cut(s) 1884, 3003, 3619, 3681, 3716
Bse21I CCTNAGG 2 cut(s) 54, 2823
Bse3DI GCAATG 2 cut(s) 2518, 3745
Bse8I GATNNNNATC 1 cut(s) 3168
BseAI TCCGGA 1 cut(s) 1421
BseBI CCWGG 7 cut(s) 138, 809, 2778, 3037, 3066, 3186, 3905
BseDI CCNNGG 3 cut(s) 1226, 2112, 3064
BseGI GGATG 9 cut(s) 137, 241, 328, 379, 817, 1119, 2046, 2231, 2965
BseJI GATNNNNATC 1 cut(s) 3168
BseMI GCAATG 2 cut(s) 2518, 3745
BseMII CTCAG 8 cut(s) 231, 377, 879, 1167, 1283, 2837, 2945, 3515
BseNI ACTGG 5 cut(s) 1884, 3003, 3619, 3681, 3716
BseRI GAGGAG 3 cut(s) 292, 1306, 4214
BseSI GKGCMC 1 cut(s) 3129
BseYI CCCAGC 3 cut(s) 2703, 3691, 3910
BsgI GTGCAG 3 cut(s) 173, 884, 2908
Bsh1285I CGRYCG 2 cut(s) 1965, 4297
BshNI GGYRCC 1 cut(s) 80
BsiEI CGRYCG 2 cut(s) 1965, 4297
BsiHKAI GWGCWC 2 cut(s) 597, 3427
BsiHKCI CYCGRG 2 cut(s) 336, 2001
BsiSI CCGG 4 cut(s) 697, 1422, 2766, 3160
BslFI GGGAC 1 cut(s) 3893
BsmAI GTCTC 4 cut(s) 337, 688, 884, 2557
BsmFI GGGAC 1 cut(s) 3893
BsmI GAATGC 3 cut(s) 994, 1487, 1534
BsoBI CYCGRG 2 cut(s) 336, 2001
Bsp119I TTCGAA 2 cut(s) 34, 3660
Bsp120I GGGCCC 1 cut(s) 3125
Bsp1286I GDGCHC 3 cut(s) 597, 3129, 3427
Bsp13I TCCGGA 1 cut(s) 1421
Bsp1407I TGTACA 3 cut(s) 47, 1241, 2378
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 6 cut(s) 175, 288, 383, 2324, 2978, 4145
BspCNI CTCAG 8 cut(s) 232, 378, 880, 1168, 1282, 2836, 2944, 3516
BspEI TCCGGA 1 cut(s) 1421
BspHI TCATGA 3 cut(s) 2425, 2905, 3895
BspMAI CTGCAG 2 cut(s) 2023, 2398
BspMI ACCTGC 2 cut(s) 2075, 2089
BspQI GCTCTTC 2 cut(s) 1854, 4200
BspT104I TTCGAA 2 cut(s) 34, 3660
BspT107I GGYRCC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 4145
BsrDI GCAATG 2 cut(s) 2518, 3745
BsrFI RCCGGY 1 cut(s) 696
BsrGI TGTACA 3 cut(s) 47, 1241, 2378
BsrI ACTGG 5 cut(s) 1884, 3003, 3619, 3681, 3716
BssAI RCCGGY 1 cut(s) 696
BssECI CCNNGG 3 cut(s) 1226, 2112, 3064
BssNI GRCGYC 1 cut(s) 2558
BssSI CACGAG 2 cut(s) 2722, 3880
BssT1I CCWWGG 2 cut(s) 1226, 2112
Bst2BI CACGAG 2 cut(s) 2722, 3880
Bst2UI CCWGG 7 cut(s) 138, 809, 2778, 3037, 3066, 3186, 3905
Bst4CI ACNGT 8 cut(s) 31, 205, 747, 766, 1414, 2738, 3334, 3445
Bst6I CTCTTC 6 cut(s) 354, 381, 1680, 1854, 3219, 4200
BstACI GRCGYC 1 cut(s) 2558
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstAUI TGTACA 3 cut(s) 47, 1241, 2378
BstBI TTCGAA 2 cut(s) 34, 3660
BstC8I GCNNGC 4 cut(s) 91, 433, 2133, 4318
BstENI CCTNNNNNAGG 3 cut(s) 1225, 2826, 3923
BstF5I GGATG 9 cut(s) 137, 241, 328, 379, 817, 1119, 2046, 2231, 2965
BstHHI GCGC 3 cut(s) 279, 2692, 4013
BstMAI GTCTC 4 cut(s) 337, 688, 884, 2557
BstMCI CGRYCG 2 cut(s) 1965, 4297
BstMWI GCNNNNNNNGC 9 cut(s) 174, 260, 1040, 1169, 1270, 1919, 2238, 3712, 3827
BstNI CCWGG 7 cut(s) 138, 809, 2778, 3037, 3066, 3186, 3905
BstNSI RCATGY 1 cut(s) 1376
BstSCI CCNGG 8 cut(s) 136, 807, 2776, 3035, 3064, 3159, 3184, 3903
BstSFI CTRYAG 5 cut(s) 453, 870, 2019, 2394, 2734
BstSLI GKGCMC 1 cut(s) 3129
BstV2I GAAGAC 5 cut(s) 767, 2049, 2794, 3323, 3353
BstX2I RGATCY 7 cut(s) 943, 980, 1573, 1990, 2762, 3356, 3509
BstXI CCANNNNNNTGG 1 cut(s) 77
BstYI RGATCY 7 cut(s) 943, 980, 1573, 1990, 2762, 3356, 3509
Bsu36I CCTNAGG 2 cut(s) 54, 2823
BtgZI GCGATG 1 cut(s) 3660
BtrI CACGTC 1 cut(s) 3865
BtsCI GGATG 9 cut(s) 137, 241, 328, 379, 817, 1119, 2046, 2231, 2965
BtsI GCAGTG 2 cut(s) 2184, 2925
BtsIMutI CAGTG 6 cut(s) 1419, 2184, 2743, 2755, 2925, 3441
BveI ACCTGC 2 cut(s) 2075, 2089
Cac8I GCNNGC 4 cut(s) 91, 433, 2133, 4318
CaiI CAGNNNCTG 2 cut(s) 2366, 3338
CciI TCATGA 3 cut(s) 2425, 2905, 3895
CfoI GCGC 3 cut(s) 279, 2692, 4013
Cfr10I RCCGGY 1 cut(s) 696
Cfr13I GGNCC 9 cut(s) 161, 1065, 1787, 1818, 3125, 3126, 3599, 3747, 3907
CseI GACGC 1 cut(s) 2566
CsiI ACCWGGT 2 cut(s) 2776, 3035
Csp6I GTAC 9 cut(s) 48, 81, 180, 1242, 1284, 2080, 2379, 2963, 4181
CviQI GTAC 9 cut(s) 48, 81, 180, 1242, 1284, 2080, 2379, 2963, 4181
DraI TTTAAA 1 cut(s) 223
DraIII CACNNNGTG 1 cut(s) 1136
EaeI YGGCCR 3 cut(s) 2718, 3388, 3901
Eam1104I CTCTTC 6 cut(s) 354, 381, 1680, 1854, 3219, 4200
EarI CTCTTC 6 cut(s) 354, 381, 1680, 1854, 3219, 4200
Ecl136II GAGCTC 2 cut(s) 595, 3425
Eco130I CCWWGG 2 cut(s) 1226, 2112
Eco147I AGGCCT 2 cut(s) 868, 2135
Eco24I GRGCYC 3 cut(s) 597, 3129, 3427
Eco32I GATATC 2 cut(s) 1021, 2452
Eco47I GGWCC 4 cut(s) 1065, 1818, 3747, 3907
Eco53kI GAGCTC 2 cut(s) 595, 3425
Eco81I CCTNAGG 2 cut(s) 54, 2823
Eco88I CYCGRG 2 cut(s) 336, 2001
EcoICRI GAGCTC 2 cut(s) 595, 3425
EcoNI CCTNNNNNAGG 3 cut(s) 1225, 2826, 3923
EcoO109I RGGNCCY 6 cut(s) 161, 1787, 1818, 3126, 3747, 3907
EcoRII CCWGG 7 cut(s) 136, 807, 2776, 3035, 3064, 3184, 3903
EcoRV GATATC 2 cut(s) 1021, 2452
EcoT14I CCWWGG 2 cut(s) 1226, 2112
EcoT22I ATGCAT 1 cut(s) 3150
EcoT38I GRGCYC 3 cut(s) 597, 3129, 3427
ErhI CCWWGG 2 cut(s) 1226, 2112
FalI AAGNNNNNCTT 6 cut(s) 351, 383, 411, 443, 1205, 1237
FaqI GGGAC 1 cut(s) 3893
FauI CCCGC 1 cut(s) 295
FauNDI CATATG 3 cut(s) 538, 2481, 2876
FbaI TGATCA 1 cut(s) 3838
FblI GTMKAC 1 cut(s) 2594
FokI GGATG 9 cut(s) 144, 248, 335, 366, 824, 1126, 2053, 2238, 2972
FriOI GRGCYC 3 cut(s) 597, 3129, 3427
FspI TGCGCA 1 cut(s) 278
GlaI GCGC 3 cut(s) 278, 2691, 4012
GsaI CCCAGC 3 cut(s) 2707, 3695, 3914
GsuI CTGGAG 4 cut(s) 609, 1938, 2570, 3441
HapII CCGG 4 cut(s) 697, 1422, 2766, 3160
HgaI GACGC 1 cut(s) 2566
HhaI GCGC 3 cut(s) 279, 2692, 4013
Hin1I GRCGYC 1 cut(s) 2558
Hin6I GCGC 3 cut(s) 277, 2690, 4011
HinP1I GCGC 3 cut(s) 277, 2690, 4011
HincII GTYRAC 5 cut(s) 2218, 3448, 3632, 3960, 4047
HindII GTYRAC 5 cut(s) 2218, 3448, 3632, 3960, 4047
HindIII AAGCTT 1 cut(s) 3819
HpaI GTTAAC 1 cut(s) 3960
HpaII CCGG 4 cut(s) 697, 1422, 2766, 3160
HphI GGTGA 6 cut(s) 678, 1040, 1479, 2780, 3025, 3233
Hpy99I CGWCG 3 cut(s) 2560, 3866, 4074
HpyCH4III ACNGT 8 cut(s) 31, 205, 747, 766, 1414, 2738, 3334, 3445
HpyCH4IV ACGT 2 cut(s) 3864, 4049
HpyF10VI GCNNNNNNNGC 9 cut(s) 174, 260, 1040, 1169, 1270, 1919, 2238, 3712, 3827
HpySE526I ACGT 2 cut(s) 3864, 4049
Hsp92I GRCGYC 1 cut(s) 2558
HspAI GCGC 3 cut(s) 277, 2690, 4011
Kpn2I TCCGGA 1 cut(s) 1421
KpnI GGTACC 1 cut(s) 84
Ksp22I TGATCA 1 cut(s) 3838
KspAI GTTAAC 1 cut(s) 3960
LguI GCTCTTC 2 cut(s) 1854, 4200
LmnI GCTCC 9 cut(s) 14, 305, 916, 1919, 2128, 2892, 3301, 3422, 4293
MabI ACCWGGT 2 cut(s) 2776, 3035
MaeII ACGT 2 cut(s) 3864, 4049
MbiI CCGCTC 1 cut(s) 4145
MfeI CAATTG 1 cut(s) 2698
MflI RGATCY 7 cut(s) 943, 980, 1573, 1990, 2762, 3356, 3509
MhlI GDGCHC 3 cut(s) 597, 3129, 3427
MlsI TGGCCA 2 cut(s) 2720, 3903
MluNI TGGCCA 2 cut(s) 2720, 3903
MlyI GAGTC 5 cut(s) 125, 1307, 1427, 1505, 2320
MmeI TCCRAC 5 cut(s) 494, 819, 909, 2226, 2291
Mox20I TGGCCA 2 cut(s) 2720, 3903
Mph1103I ATGCAT 1 cut(s) 3150
MroI TCCGGA 1 cut(s) 1421
MroXI GAANNNNTTC 2 cut(s) 1944, 3026
MscI TGGCCA 2 cut(s) 2720, 3903
MslI CAYNNNNRTG 3 cut(s) 1377, 1955, 3456
Msp20I TGGCCA 2 cut(s) 2720, 3903
MspA1I CMGCKG 4 cut(s) 177, 385, 1175, 2024
MspI CCGG 4 cut(s) 697, 1422, 2766, 3160
MspR9I CCNGG 8 cut(s) 138, 809, 2778, 3037, 3066, 3161, 3186, 3905
MunI CAATTG 1 cut(s) 2698
Mva1269I GAATGC 3 cut(s) 994, 1487, 1534
MvaI CCWGG 7 cut(s) 138, 809, 2778, 3037, 3066, 3186, 3905
MwoI GCNNNNNNNGC 9 cut(s) 174, 260, 1040, 1169, 1270, 1919, 2238, 3712, 3827
NciI CCSGG 1 cut(s) 3161
NdeI CATATG 3 cut(s) 538, 2481, 2876
NmuCI GTSAC 4 cut(s) 25, 1852, 3328, 3542
NsbI TGCGCA 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 3150
NspI RCATGY 1 cut(s) 1376
NspV TTCGAA 2 cut(s) 34, 3660
PaeR7I CTCGAG 1 cut(s) 2001
PagI TCATGA 3 cut(s) 2425, 2905, 3895
PceI AGGCCT 2 cut(s) 868, 2135
PciSI GCTCTTC 2 cut(s) 1854, 4200
PctI GAATGC 3 cut(s) 994, 1487, 1534
PdmI GAANNNNTTC 2 cut(s) 1944, 3026
PflMI CCANNNNNTGG 1 cut(s) 1814
PfoI TCCNGGA 3 cut(s) 136, 807, 3159
Ple19I CGATCG 1 cut(s) 4297
PleI GAGTC 5 cut(s) 124, 1307, 1426, 1504, 2319
PpsI GAGTC 5 cut(s) 124, 1307, 1426, 1504, 2319
PpuMI RGGWCCY 3 cut(s) 1818, 3747, 3907
Psp124BI GAGCTC 2 cut(s) 597, 3427
Psp5II RGGWCCY 3 cut(s) 1818, 3747, 3907
Psp6I CCWGG 7 cut(s) 136, 807, 2776, 3035, 3064, 3184, 3903
PspFI CCCAGC 3 cut(s) 2703, 3691, 3910
PspGI CCWGG 7 cut(s) 136, 807, 2776, 3035, 3064, 3184, 3903
PspOMI GGGCCC 1 cut(s) 3125
PspPI GGNCC 9 cut(s) 161, 1065, 1787, 1818, 3125, 3126, 3599, 3747, 3907
PspPPI RGGWCCY 3 cut(s) 1818, 3747, 3907
PstI CTGCAG 2 cut(s) 2023, 2398
PstNI CAGNNNCTG 2 cut(s) 2366, 3338
PsuI RGATCY 7 cut(s) 943, 980, 1573, 1990, 2762, 3356, 3509
PvuI CGATCG 1 cut(s) 4297
PvuII CAGCTG 2 cut(s) 1175, 2024
RsaI GTAC 9 cut(s) 49, 82, 181, 1243, 1285, 2081, 2380, 2964, 4182
RsaNI GTAC 9 cut(s) 48, 81, 180, 1242, 1284, 2080, 2379, 2963, 4181
RseI CAYNNNNRTG 3 cut(s) 1377, 1955, 3456
SacI GAGCTC 2 cut(s) 597, 3427
SapI GCTCTTC 2 cut(s) 1854, 4200
Sau96I GGNCC 9 cut(s) 161, 1065, 1787, 1818, 3125, 3126, 3599, 3747, 3907
SchI GAGTC 5 cut(s) 125, 1307, 1427, 1505, 2320
ScrFI CCNGG 8 cut(s) 138, 809, 2778, 3037, 3066, 3161, 3186, 3905
SduI GDGCHC 3 cut(s) 597, 3129, 3427
SexAI ACCWGGT 2 cut(s) 2776, 3035
SfcI CTRYAG 5 cut(s) 453, 870, 2019, 2394, 2734
Sfr274I CTCGAG 1 cut(s) 2001
SfuI TTCGAA 2 cut(s) 34, 3660
SinI GGWCC 4 cut(s) 1065, 1818, 3747, 3907
SlaI CTCGAG 1 cut(s) 2001
SmiMI CAYNNNNRTG 3 cut(s) 1377, 1955, 3456
SmlI CTYRAG 5 cut(s) 101, 862, 2001, 3324, 3754
SmoI CTYRAG 5 cut(s) 101, 862, 2001, 3324, 3754
SseBI AGGCCT 2 cut(s) 868, 2135
SsiI CCGC 6 cut(s) 175, 288, 383, 2324, 2978, 4145
SspI AATATT 3 cut(s) 347, 2198, 3787
SstI GAGCTC 2 cut(s) 597, 3427
StuI AGGCCT 2 cut(s) 868, 2135
StyD4I CCNGG 8 cut(s) 136, 807, 2776, 3035, 3064, 3159, 3184, 3903
StyI CCWWGG 2 cut(s) 1226, 2112
TaaI ACNGT 8 cut(s) 31, 205, 747, 766, 1414, 2738, 3334, 3445
TaiI ACGT 2 cut(s) 3867, 4052
TaqII GACCGA 3 cut(s) 1676, 1951, 1955
TatI WGTACW 6 cut(s) 47, 1241, 1283, 2079, 2378, 2962
TauI GCSGC 1 cut(s) 2327
TscAI CASTG 6 cut(s) 1419, 2184, 2743, 2755, 2932, 3448
TseFI GTSAC 4 cut(s) 25, 1852, 3328, 3542
Tsp45I GTSAC 4 cut(s) 25, 1852, 3328, 3542
TspGWI ACGGA 4 cut(s) 1776, 2824, 3841, 3945
TspRI CASTG 6 cut(s) 1419, 2184, 2743, 2755, 2932, 3448
Van91I CCANNNNNTGG 1 cut(s) 1814
VpaK11BI GGWCC 4 cut(s) 1065, 1818, 3747, 3907
XagI CCTNNNNNAGG 3 cut(s) 1225, 2826, 3923
XapI RAATTY 6 cut(s) 1642, 2541, 2998, 4033, 4152, 4247
XbaI TCTAGA 2 cut(s) 1297, 3175
XceI RCATGY 1 cut(s) 1376
XhoI CTCGAG 1 cut(s) 2001
XmiI GTMKAC 1 cut(s) 2594
XmnI GAANNNNTTC 2 cut(s) 1944, 3026
Zsp2I ATGCAT 1 cut(s) 3150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.