pycom05g25390

Small RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002993
Physical Location & Seq
Reverse (-)
26756056 .. 26763608
7553 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25390.3

Sequence Viewer

Length: 3087 bp
ATGTGCGGTCCACTTGTGTTAGAGTCTTTGCTTTCCTTCCTCCAAAACCTCTCTCTCTCTCTCTCTCTCTCTATATCGTTTCTGCTGCCCCACCAAAACCCCACCAACGCACACATTATTCACTCTCTCTCTCTGCTCTGTGCCTCTCTCTCTCCCTCCTCTCTCAGGCTCGGCCATTGCCGTTGTGAATGCGAAAAGTTGAAGACCTCTTGGGTACAGATGGAAACTGAAGGAGCCGCAACTGTTGCAGTGCGAAACACTACCTTTACGCCTAAGGCTATCATACACAAAAAGTTCGGTAGCACAGCCTGCTATGAAGTAGAGGAAGTGCATGAGTCTCCTCAAAATGGATGTCCAGGGTTGGCCATTATGCAGAAGGGCCCTTGCCTTTATCGCTGTACCTTGAAACTTCCAGAAGTTACTGTCGTATCTGGAACCTTTAAAAAGAAGAAGGACGCAGAACAGTCTGCCGCTGAATTGGCCTTAGAAAAGCTAGGCATTAATCCCTCAACAAAAGTCCCTACTCTGCAGGAAGCATGGGATGAGTTAGTCTCTCGTGTCAATTTTTTATTTTCAGACGAGTTTCTTCCAGCTCTTCATCCTCTGAGTGGTCACTTCAGAGCAGCATTGCAGAGAGAAGGTGATCTTTCTGGTCAAATTCCTGCTGCAGTCATTGCTGTCTTTGACGCAACACTTTTTAATATGTGTAAATCCATTAATCCCAAGGTGGAGTCAATTCCTTTCTTGGCTATATTATATGTTATGAGGGCTGCTGCTAAATTATCTGGACTTCTTGCTACTTCTGAGGAAGATCTCTGGATTAGGAGGAAAAATCCATACACTCCTGAAATAATAGAGTCATCATCTGTTGAGCAATCAGACTCCAAAGAAATATTTCCGATTGAAGCCATAAATGTACCATGTTCATTAGAGAAGAGTGTTGAAAGAGTAATCCTAAATCTCTCATCTTCTGGATATTTCCTGGATGTTATAGCAAAACAACTTGGGTTATTGAACGCTGCTGACATTCTGATCTCAAGGCCTATAGGTAAAGCTTCCTCTGAGACAAGACTGTACTTTGCTGCTCCAAAGAAATCTCTGTTGGATATCTCTTCAGATCTTCTAAATGCCAAAGGAGCTTGTAATTCTGAAGGATCTTTCAATGCAAGAGCAAGTTACCTGTCGGGCCAAGATATATATGGTGACGCTGTTTTGGCATCCATTGGATACACATGGAGGTCCAAAGACCTTTTCTATGAAGATGTATCCCTGCAATCATATCACAGGATGGTCATTGGCAAGACACCAAGTGGAATTTACAAGTTGTCCAGAGAAGCAATACTTGCAGCTGAGTTGCCTTTAGCATTTACTGCTAAGGCAAAATGGAAGGGTTCGATTCCAAGGGAGATACTGTGTACATTCTGCCGCCAGCACCGGCTTGAGCCCATCTTCTCTCCTCAAAACACTCTAGAAGAATCATCCGAGTCACCAATATCACACAAGAAGTTAAAGGTGACAGACTTGGCTGGGAAAGAGACACAATATGCAAATGGCTGTGTTGTTGATGCTGGTGTAAAGGAGATAGTTGAATCCGGAGGTAGCTTTAGATGTGAAGTAAAAATAATTTCTAAGTTTCAGGATTTTATTTTAGAGTGCTCACCAAAAGAGACTTTTAAGAAGCAGAGTGATGCCATCCAGAATGTTTCTTTGAAAGTTCTGTTATGGTTAAATGCATATTTTGGGGATCTAACTATGCCTTTAGAGAGACTAAACGCTTCTGCTGATGGCCACAACATTAGATTCGATTCCCAAAACTTTATTAAAACATTTATGCTGTGCCATCATATTCATACTGTTGGGCATAAAGAGACTGAAGAAGGAAAATTATCATTTTCCAACTCTGTGAATGCACTGTTTGCTCTCCCTGGAATTGAAATTTGTTCCGTAAGTATAGAAGGACCGGATTCTGGTGTTTGTCCATCTATTGGGTCCTTAGCATGTGTAAGCTATTCTACTTCTTTGGTGACTGAAGGCAAACATACGAAAGAACTTTTGGAAAGTAGTGATGACTTTGAGTTTGAGATAGCATCTGGAGCAGTGATCCCACATCTCGAGTCAGTTATTACACAAATAACTGTTGGTCAGTCGGCTTTTTTCAGTATGGATTTGCCACCTCAAGAGTTGGTTTTAGCCGCAGCTGGTGATTCTGCTAGGATGCTTTCCTTATTGTCTTCAGAAACCTGCTATTTGGAGTACACTATAACTTTGTTGGGGGTAACCGAACCACTAGAGGATAGAATGGAGCAGGCTTTTTTCAGCCCTCCACTTTCAAAACAGCGTGTGGAATATGCAGTGCAAAGCATCAAAGAATCTTGTGCAACTACTTTGGTTGACTTTGGATGTGGCTCTGGTAGTTTGTTGGATTCTTTATTAAACTATCCAACTTCTCTGGAAAAAATTGCTGGTGTTGATATTTCACGAAAGAGTCTTGCTCGTGCGGCAAAGATACTTCATTCCAAATTAAACTCAAATTCAGATGCCATTACGTCAGCCATGAATTCAGCAATTCTTTACGATGGTTCAATTACAAATTTTGATTCCCGGTTGAGTGGATTTGATATTGGCACTTGCTTAGAGGTAATAGAGCATATGGAGGAAGATCAAGCATCTGAGTTTGGCAACGTGGTGCTAAGTTTATTTCGTCCAAGGGTTCTTATTGTCTCCACTCCAAACTACGAATACAATGTGATACTCCAGAAATCCAATTTGTCAAGCCAAGAAGATGACCCGGAGGACAAAAGTCAAGCACAATGTAAATTTCGTAACCATGACCACAAGTTCGAGTGGACGAGAGAGCAGTTCAATTGCTGGGCGACTGAATTGGCTGCGAAGCACAACTACAGTGTTGAGTTCAGTGGAGTTGGGGGTTCCGGTGATACAGAACCAGGTTTTGCTTCTCAGATTGCTGTCTTTAGACAGGAACACGTAACCAAGGAAGTTGCTTTTCCAGAGGTTTCAGATCTGGAACAACCTTTCGAAGTTATATGGGAATGGAATAGCAGCGATAGGCCAGGATCTGCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001510 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006379 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009653 GO:0009791 GO:0009892 GO:0009908 GO:0009909 GO:0009954 GO:0009987 GO:0010033 GO:0010051 GO:0010093 GO:0010305 GO:0010467 GO:0010468 GO:0010589 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016740 GO:0016741 GO:0019222 GO:0022414 GO:0031047 GO:0031050 GO:0032259 GO:0032501 GO:0032502 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035279 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048519 GO:0048580 GO:0048608 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0098542 GO:0098586 GO:0098795 GO:0099402 GO:0140098 GO:1901360 GO:1901698 GO:1901699 GO:1905392 GO:1905393 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKBP_C Methyltransf_11 Methyltransf_12 Methyltransf_31 dsrm

Protein Analysis

1029

Amino Acids

112.93

Weight (kDa)

5.28

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2249
AccB7I CCANNNNNTGG 1 cut(s) 1985
AccIII TCCGGA 1 cut(s) 1592
AciI CCGC 6 cut(s) 6, 237, 471, 1426, 2193, 2498
AclWI GGATC 4 cut(s) 1162, 1752, 2095, 3082
AcoI YGGCCR 3 cut(s) 172, 363, 1786
AcsI RAATTY 7 cut(s) 657, 1314, 1935, 2530, 2557, 2590, 2816
AcuI CTGAAG 7 cut(s) 249, 601, 1098, 1170, 1893, 2049, 2217
AdeI CACNNNGTG 1 cut(s) 1310
AfaI GTAC 6 cut(s) 216, 400, 918, 1076, 1417, 2255
AfiI CCNNNNNNNGG 5 cut(s) 165, 347, 608, 1967, 1985
AflIII ACRYGT 1 cut(s) 2983
AjnI CCWGG 5 cut(s) 355, 981, 1924, 2944, 3070
AjuI GAANNNNNNNTTGG 6 cut(s) 1085, 1117, 1235, 1267, 2036, 2068
AluBI AGCT 8 cut(s) 493, 593, 1055, 1139, 1349, 1602, 2007, 2198
AluI AGCT 8 cut(s) 493, 593, 1055, 1139, 1349, 1602, 2007, 2198
Alw21I GWGCWC 1 cut(s) 1658
Alw26I GTCTC 8 cut(s) 342, 556, 1058, 1529, 1661, 1759, 1862, 2725
AlwI GGATC 4 cut(s) 1162, 1752, 2095, 3082
AlwNI CAGNNNCTG 1 cut(s) 3077
Ama87I CYCGRG 1 cut(s) 2111
Aor13HI TCCGGA 1 cut(s) 1592
AoxI GGCC 8 cut(s) 172, 363, 379, 480, 1040, 1186, 1786, 3068
ApaI GGGCCC 1 cut(s) 383
ApoI RAATTY 7 cut(s) 657, 1314, 1935, 2530, 2557, 2590, 2816
ArsI GACNNNNNNTTYG 6 cut(s) 337, 369, 2060, 2092, 2822, 2854
AseI ATTAAT 2 cut(s) 501, 717
Asp700I GAANNNNTTC 1 cut(s) 894
AspS9I GGNCC 7 cut(s) 8, 379, 380, 1186, 1239, 1958, 1989
AsuC2I CCSGG 2 cut(s) 2602, 2789
AsuHPI GGTGA 8 cut(s) 653, 1214, 1479, 1525, 1650, 2035, 2213, 2945
AsuII TTCGAA 1 cut(s) 3036
AvaI CYCGRG 1 cut(s) 2111
AvaII GGWCC 4 cut(s) 8, 1239, 1958, 1989
AxyI CCTNAGG 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 383
BaeI ACNNNNGTAYC 2 cut(s) 411, 444
BalI TGGCCA 2 cut(s) 365, 1788
BanII GRGCYC 2 cut(s) 383, 1446
BarI GAAGNNNNNNTAC 2 cut(s) 2882, 2914
BauI CACGAG 2 cut(s) 555, 2493
BbsI GAAGAC 2 cut(s) 209, 2223
Bbv12I GWGCWC 1 cut(s) 1658
BccI CCATC 8 cut(s) 214, 1282, 1454, 1700, 1778, 1848, 1987, 2570
BceAI ACGGC 1 cut(s) 165
BciT130I CCWGG 5 cut(s) 357, 983, 1926, 2946, 3072
BciVI GTATCC 2 cut(s) 1220, 1276
BcnI CCSGG 2 cut(s) 2602, 2789
BcoDI GTCTC 8 cut(s) 342, 556, 1058, 1529, 1661, 1759, 1862, 2725
BfaI CTAG 4 cut(s) 494, 1469, 2211, 2288
BfmI CTRYAG 4 cut(s) 527, 666, 1044, 2899
BfuAI ACCTGC 1 cut(s) 2249
BfuI GTATCC 2 cut(s) 1220, 1276
BglII AGATCT 3 cut(s) 811, 1117, 3019
Bme1390I CCNGG 7 cut(s) 357, 983, 1926, 2602, 2789, 2946, 3072
Bme18I GGWCC 4 cut(s) 8, 1239, 1958, 1989
BmeT110I CYCGRG 1 cut(s) 2111
BmgT120I GGNCC 7 cut(s) 8, 379, 380, 1186, 1239, 1958, 1989
BmiI GGNNCC 5 cut(s) 235, 381, 436, 1990, 2929
BmrFI CCNGG 7 cut(s) 357, 983, 1926, 2602, 2789, 2946, 3072
BmsI GCATC 8 cut(s) 1226, 1555, 1678, 2096, 2205, 2370, 2527, 2675
BoxI GACNNNNGTC 1 cut(s) 2799
BpiI GAAGAC 2 cut(s) 209, 2223
BplI GAGNNNNNCTC 4 cut(s) 536, 568, 2476, 2508
BpmI CTGGAG 2 cut(s) 2112, 2738
Bpu10I CCTNAGC 2 cut(s) 1374, 1993
Bpu14I TTCGAA 1 cut(s) 3036
BpuEI CTTGAG 3 cut(s) 1021, 1460, 2160
BpuMI CCSGG 2 cut(s) 2602, 2789
BsaAI YACGTR 1 cut(s) 2986
BsaJI CCNNGG 6 cut(s) 356, 723, 1400, 1924, 2705, 2991
BsaWI WCCGGW 3 cut(s) 1592, 1960, 2930
BsaXI ACNNNNNCTCC 2 cut(s) 1397, 1427
Bsc4I CCNNNNNNNGG 5 cut(s) 165, 347, 608, 1967, 1985
Bse118I RCCGGY 1 cut(s) 1434
Bse21I CCTNAGG 1 cut(s) 273
Bse3DI GCAATG 3 cut(s) 175, 626, 672
BseAI TCCGGA 1 cut(s) 1592
BseBI CCWGG 5 cut(s) 357, 983, 1926, 2946, 3072
BseDI CCNNGG 6 cut(s) 356, 723, 1400, 1924, 2705, 2991
BseLI CCNNNNNNNGG 5 cut(s) 165, 347, 608, 1967, 1985
BseMI GCAATG 3 cut(s) 175, 626, 672
BseMII CTCAG 7 cut(s) 178, 596, 795, 1053, 1341, 2661, 2972
BseRI GAGGAG 3 cut(s) 148, 330, 1446
BseSI GKGCMC 1 cut(s) 383
BseYI CCCAGC 2 cut(s) 1526, 2868
BshFI GGCC 8 cut(s) 174, 365, 381, 482, 1042, 1188, 1788, 3070
BsiHKAI GWGCWC 1 cut(s) 1658
BsiHKCI CYCGRG 1 cut(s) 2111
BsiSI CCGG 6 cut(s) 1435, 1593, 1961, 2602, 2789, 2931
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 5 cut(s) 165, 347, 608, 1967, 1985
BsmAI GTCTC 8 cut(s) 342, 556, 1058, 1529, 1661, 1759, 1862, 2725
BsmFI GGGAC 1 cut(s) 503
BsmI GAATGC 2 cut(s) 194, 1912
BsnI GGCC 8 cut(s) 174, 365, 381, 482, 1042, 1188, 1788, 3070
BsoBI CYCGRG 1 cut(s) 2111
Bsp119I TTCGAA 1 cut(s) 3036
Bsp120I GGGCCC 1 cut(s) 379
Bsp1286I GDGCHC 3 cut(s) 383, 1446, 1658
Bsp13I TCCGGA 1 cut(s) 1592
Bsp1407I TGTACA 1 cut(s) 1415
BspACI CCGC 6 cut(s) 6, 237, 471, 1426, 2193, 2498
BspANI GGCC 8 cut(s) 174, 365, 381, 482, 1042, 1188, 1788, 3070
BspCNI CTCAG 7 cut(s) 177, 597, 796, 1054, 1342, 2662, 2971
BspEI TCCGGA 1 cut(s) 1592
BspLI GGNNCC 5 cut(s) 235, 381, 436, 1990, 2929
BspMAI CTGCAG 2 cut(s) 531, 670
BspMI ACCTGC 1 cut(s) 2249
BspPI GGATC 4 cut(s) 1162, 1752, 2095, 3082
BspQI GCTCTTC 1 cut(s) 600
BspT104I TTCGAA 1 cut(s) 3036
BsrDI GCAATG 3 cut(s) 175, 626, 672
BsrFI RCCGGY 1 cut(s) 1434
BsrGI TGTACA 1 cut(s) 1415
BssAI RCCGGY 1 cut(s) 1434
BssECI CCNNGG 6 cut(s) 356, 723, 1400, 1924, 2705, 2991
BssSI CACGAG 2 cut(s) 555, 2493
BssT1I CCWWGG 4 cut(s) 723, 1400, 2705, 2991
Bst2BI CACGAG 2 cut(s) 555, 2493
Bst2UI CCWGG 5 cut(s) 357, 983, 1926, 2946, 3072
Bst4CI ACNGT 9 cut(s) 244, 424, 465, 1074, 1413, 1855, 1914, 2137, 2903
Bst6I CTCTTC 3 cut(s) 600, 929, 1117
BstAPI GCANNNNNTGC 6 cut(s) 245, 309, 674, 1343, 1370, 1916
BstAUI TGTACA 1 cut(s) 1415
BstBAI YACGTR 1 cut(s) 2986
BstBI TTCGAA 1 cut(s) 3036
BstC8I GCNNGC 3 cut(s) 310, 1430, 2307
BstEII GGTNACC 1 cut(s) 2275
BstENI CCTNNNNNAGG 1 cut(s) 163
BstMAI GTCTC 8 cut(s) 342, 556, 1058, 1529, 1661, 1759, 1862, 2725
BstNI CCWGG 5 cut(s) 357, 983, 1926, 2946, 3072
BstNSI RCATGY 1 cut(s) 2001
BstPAI GACNNNNGTC 1 cut(s) 2799
BstPI GGTNACC 1 cut(s) 2275
BstSCI CCNGG 7 cut(s) 355, 981, 1924, 2600, 2787, 2944, 3070
BstSFI CTRYAG 4 cut(s) 527, 666, 1044, 2899
BstSLI GKGCMC 1 cut(s) 383
BstV2I GAAGAC 2 cut(s) 209, 2223
BstX2I RGATCY 6 cut(s) 811, 1117, 1154, 1744, 3019, 3074
BstYI RGATCY 6 cut(s) 811, 1117, 1154, 1744, 3019, 3074
Bsu36I CCTNAGG 1 cut(s) 273
BsuI GTATCC 2 cut(s) 1220, 1276
BsuRI GGCC 8 cut(s) 174, 365, 381, 482, 1042, 1188, 1788, 3070
BtsI GCAGTG 3 cut(s) 255, 2103, 2358
BtsIMutI CAGTG 6 cut(s) 255, 1910, 2103, 2358, 2908, 2920
BveI ACCTGC 1 cut(s) 2249
Cac8I GCNNGC 3 cut(s) 310, 1430, 2307
CaiI CAGNNNCTG 1 cut(s) 3077
Cfr10I RCCGGY 1 cut(s) 1434
Cfr13I GGNCC 7 cut(s) 8, 379, 380, 1186, 1239, 1958, 1989
CseI GACGC 3 cut(s) 464, 695, 1214
CsiI ACCWGGT 1 cut(s) 2944
Csp6I GTAC 6 cut(s) 215, 399, 917, 1075, 1416, 2254
CviAII CATG 7 cut(s) 332, 537, 921, 1233, 1998, 2554, 2828
CviQI GTAC 6 cut(s) 215, 399, 917, 1075, 1416, 2254
DraI TTTAAA 1 cut(s) 442
DraIII CACNNNGTG 1 cut(s) 1310
EaeI YGGCCR 3 cut(s) 172, 363, 1786
Eam1104I CTCTTC 3 cut(s) 600, 929, 1117
EarI CTCTTC 3 cut(s) 600, 929, 1117
Eco130I CCWWGG 4 cut(s) 723, 1400, 2705, 2991
Eco147I AGGCCT 1 cut(s) 1042
Eco24I GRGCYC 2 cut(s) 383, 1446
Eco32I GATATC 1 cut(s) 1108
Eco47I GGWCC 4 cut(s) 8, 1239, 1958, 1989
Eco57I CTGAAG 7 cut(s) 249, 601, 1098, 1170, 1893, 2049, 2217
Eco81I CCTNAGG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 2111
Eco91I GGTNACC 1 cut(s) 2275
EcoNI CCTNNNNNAGG 1 cut(s) 163
EcoO109I RGGNCCY 3 cut(s) 379, 380, 1989
EcoO65I GGTNACC 1 cut(s) 2275
EcoRI GAATTC 1 cut(s) 2557
EcoRII CCWGG 5 cut(s) 355, 981, 1924, 2944, 3070
EcoRV GATATC 1 cut(s) 1108
EcoT14I CCWWGG 4 cut(s) 723, 1400, 2705, 2991
EcoT22I ATGCAT 1 cut(s) 1735
EcoT38I GRGCYC 2 cut(s) 383, 1446
ErhI CCWWGG 4 cut(s) 723, 1400, 2705, 2991
FaeI CATG 7 cut(s) 335, 540, 924, 1236, 2001, 2557, 2831
FalI AAGNNNNNCTT 4 cut(s) 630, 662, 1326, 1358
FaqI GGGAC 1 cut(s) 503
FatI CATG 7 cut(s) 331, 536, 920, 1232, 1997, 2553, 2827
FauNDI CATATG 1 cut(s) 2649
FriOI GRGCYC 2 cut(s) 383, 1446
FspBI CTAG 4 cut(s) 494, 1469, 2211, 2288
GsaI CCCAGC 2 cut(s) 1530, 2872
GsuI CTGGAG 2 cut(s) 2112, 2738
HaeIII GGCC 8 cut(s) 174, 365, 381, 482, 1042, 1188, 1788, 3070
HapII CCGG 6 cut(s) 1435, 1593, 1961, 2602, 2789, 2931
HgaI GACGC 3 cut(s) 464, 695, 1214
Hin1II CATG 7 cut(s) 335, 540, 924, 1236, 2001, 2557, 2831
HincII GTYRAC 1 cut(s) 2392
HindII GTYRAC 1 cut(s) 2392
HindIII AAGCTT 1 cut(s) 1053
HpaII CCGG 6 cut(s) 1435, 1593, 1961, 2602, 2789, 2931
HphI GGTGA 8 cut(s) 653, 1214, 1479, 1525, 1650, 2035, 2213, 2945
Hpy166II GTNNAC 5 cut(s) 11, 1416, 2256, 2392, 2847
Hpy8I GTNNAC 5 cut(s) 11, 1416, 2256, 2392, 2847
HpyCH4III ACNGT 9 cut(s) 244, 424, 465, 1074, 1413, 1855, 1914, 2137, 2903
HpyCH4IV ACGT 3 cut(s) 2546, 2682, 2985
HpySE526I ACGT 3 cut(s) 2546, 2682, 2985
Hsp92II CATG 7 cut(s) 335, 540, 924, 1236, 2001, 2557, 2831
Kpn2I TCCGGA 1 cut(s) 1592
LguI GCTCTTC 1 cut(s) 600
LmnI GCTCC 5 cut(s) 233, 1090, 1136, 2093, 2302
LweI GCATC 8 cut(s) 1226, 1555, 1678, 2096, 2205, 2370, 2527, 2675
MabI ACCWGGT 1 cut(s) 2944
MaeI CTAG 4 cut(s) 494, 1469, 2211, 2288
MaeII ACGT 3 cut(s) 2546, 2682, 2985
MfeI CAATTG 1 cut(s) 2863
MflI RGATCY 6 cut(s) 811, 1117, 1154, 1744, 3019, 3074
MhlI GDGCHC 3 cut(s) 383, 1446, 1658
MlsI TGGCCA 2 cut(s) 365, 1788
MluNI TGGCCA 2 cut(s) 365, 1788
MlyI GAGTC 8 cut(s) 32, 344, 740, 866, 875, 1493, 2123, 2494
MmeI TCCRAC 4 cut(s) 1083, 1920, 2400, 2465
Mox20I TGGCCA 2 cut(s) 365, 1788
Mph1103I ATGCAT 1 cut(s) 1735
MroI TCCGGA 1 cut(s) 1592
MroXI GAANNNNTTC 1 cut(s) 894
MscI TGGCCA 2 cut(s) 365, 1788
Msp20I TGGCCA 2 cut(s) 365, 1788
MspA1I CMGCKG 3 cut(s) 473, 1349, 2198
MspI CCGG 6 cut(s) 1435, 1593, 1961, 2602, 2789, 2931
MspR9I CCNGG 7 cut(s) 357, 983, 1926, 2602, 2789, 2946, 3072
MunI CAATTG 1 cut(s) 2863
Mva1269I GAATGC 2 cut(s) 194, 1912
MvaI CCWGG 5 cut(s) 357, 983, 1926, 2946, 3072
NciI CCSGG 2 cut(s) 2602, 2789
NdeI CATATG 1 cut(s) 2649
NlaIII CATG 7 cut(s) 335, 540, 924, 1236, 2001, 2557, 2831
NlaIV GGNNCC 5 cut(s) 235, 381, 436, 1990, 2929
NmeAIII GCCGAG 1 cut(s) 150
NmuCI GTSAC 5 cut(s) 611, 1202, 1485, 1513, 2023
NsiI ATGCAT 1 cut(s) 1735
NspI RCATGY 1 cut(s) 2001
NspV TTCGAA 1 cut(s) 3036
PaeR7I CTCGAG 1 cut(s) 2111
PceI AGGCCT 1 cut(s) 1042
PciSI GCTCTTC 1 cut(s) 600
PctI GAATGC 2 cut(s) 194, 1912
PdmI GAANNNNTTC 1 cut(s) 894
PflMI CCANNNNNTGG 1 cut(s) 1985
PfoI TCCNGGA 1 cut(s) 981
PleI GAGTC 8 cut(s) 31, 343, 739, 865, 875, 1492, 2122, 2493
PpsI GAGTC 8 cut(s) 31, 343, 739, 865, 875, 1492, 2122, 2493
Ppu21I YACGTR 1 cut(s) 2986
PpuMI RGGWCCY 1 cut(s) 1989
PshAI GACNNNNGTC 1 cut(s) 2799
PshBI ATTAAT 2 cut(s) 501, 717
Psp5II RGGWCCY 1 cut(s) 1989
Psp6I CCWGG 5 cut(s) 355, 981, 1924, 2944, 3070
PspEI GGTNACC 1 cut(s) 2275
PspFI CCCAGC 2 cut(s) 1526, 2868
PspGI CCWGG 5 cut(s) 355, 981, 1924, 2944, 3070
PspN4I GGNNCC 5 cut(s) 235, 381, 436, 1990, 2929
PspOMI GGGCCC 1 cut(s) 379
PspPI GGNCC 7 cut(s) 8, 379, 380, 1186, 1239, 1958, 1989
PspPPI RGGWCCY 1 cut(s) 1989
PstI CTGCAG 2 cut(s) 531, 670
PstNI CAGNNNCTG 1 cut(s) 3077
PsuI RGATCY 6 cut(s) 811, 1117, 1154, 1744, 3019, 3074
PvuII CAGCTG 2 cut(s) 1349, 2198
RsaI GTAC 6 cut(s) 216, 400, 918, 1076, 1417, 2255
RsaNI GTAC 6 cut(s) 215, 399, 917, 1075, 1416, 2254
SapI GCTCTTC 1 cut(s) 600
Sau96I GGNCC 7 cut(s) 8, 379, 380, 1186, 1239, 1958, 1989
SchI GAGTC 8 cut(s) 32, 344, 740, 866, 875, 1493, 2123, 2494
ScrFI CCNGG 7 cut(s) 357, 983, 1926, 2602, 2789, 2946, 3072
SduI GDGCHC 3 cut(s) 383, 1446, 1658
SexAI ACCWGGT 1 cut(s) 2944
SfaNI GCATC 8 cut(s) 1226, 1555, 1678, 2096, 2205, 2370, 2527, 2675
SfcI CTRYAG 4 cut(s) 527, 666, 1044, 2899
Sfr274I CTCGAG 1 cut(s) 2111
SfuI TTCGAA 1 cut(s) 3036
SinI GGWCC 4 cut(s) 8, 1239, 1958, 1989
SlaI CTCGAG 1 cut(s) 2111
SmlI CTYRAG 4 cut(s) 1036, 1439, 2111, 2175
SmoI CTYRAG 4 cut(s) 1036, 1439, 2111, 2175
SseBI AGGCCT 1 cut(s) 1042
SsiI CCGC 6 cut(s) 6, 237, 471, 1426, 2193, 2498
SspI AATATT 1 cut(s) 894
SspMI CTAG 4 cut(s) 494, 1469, 2211, 2288
StuI AGGCCT 1 cut(s) 1042
StyD4I CCNGG 7 cut(s) 355, 981, 1924, 2600, 2787, 2944, 3070
StyI CCWWGG 4 cut(s) 723, 1400, 2705, 2991
TaaI ACNGT 9 cut(s) 244, 424, 465, 1074, 1413, 1855, 1914, 2137, 2903
TaiI ACGT 3 cut(s) 2549, 2685, 2988
TaqI TCGA 5 cut(s) 1394, 1803, 2112, 2841, 3036
TatI WGTACW 3 cut(s) 1074, 1415, 2253
TauI GCSGC 5 cut(s) 239, 473, 1428, 2195, 2501
TscAI CASTG 6 cut(s) 255, 1917, 2103, 2358, 2908, 2920
TseFI GTSAC 5 cut(s) 611, 1202, 1485, 1513, 2023
Tsp45I GTSAC 5 cut(s) 611, 1202, 1485, 1513, 2023
TspDTI ATGAA 7 cut(s) 330, 587, 915, 1272, 1838, 2501, 2570
TspGWI ACGGA 1 cut(s) 1933
TspRI CASTG 6 cut(s) 255, 1917, 2103, 2358, 2908, 2920
Van91I CCANNNNNTGG 1 cut(s) 1985
VpaK11BI GGWCC 4 cut(s) 8, 1239, 1958, 1989
VspI ATTAAT 2 cut(s) 501, 717
XagI CCTNNNNNAGG 1 cut(s) 163
XapI RAATTY 7 cut(s) 657, 1314, 1935, 2530, 2557, 2590, 2816
XbaI TCTAGA 1 cut(s) 1468
XceI RCATGY 1 cut(s) 2001
XcmI CCANNNNNNNNNTGG 1 cut(s) 1196
XhoI CTCGAG 1 cut(s) 2111
XmnI GAANNNNTTC 1 cut(s) 894
XspI CTAG 4 cut(s) 494, 1469, 2211, 2288
Zsp2I ATGCAT 1 cut(s) 1735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.