RchiOBHm_Chr5g0016471

Small RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002993
Physical Location & Seq
Forward (+)
11320485 .. 11326920
6436 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29684

Sequence Viewer

Length: 2946 bp
ATGGAAACTGAAGGAGCCTCATCTGTCACAGTTCGAAAGACTAGCTGTACACCTAAGGCTATTATACACCAAAAGTTTGGTACCAATGCGTGCTATAAACATGAGGAAGTGCATGAGTCTGCTGAAAATGGATGTCCAGGATTAGCCATTGTGCAGAAAGGCCCTTGCCTTTACCGCTGTACCTTGCAGCTTCCAGAAATTACTGTTGTATCTGGAATCTTTAAAAAGAAGAAGGATGCTGAGCAATCTGCTGCTGAATTGGCCTTAGAAAAGCTGCGCATCACCCCCGCCACAAAAAGTTCCTCTCTTCCAGAAGCATGGGATGGTTTAGTCTCTCGGGTCAAATATTTATTTTCAGAAGAGTTCCTTTCATCTCTTCATCCGCTGAGTGGTCATTTGAGAGCAGCTTTGCAAAGAGAAGGTCATCTTGCTGGCTTGATCCCTGCTTGTGCTATAGCCGTCTTTGATGCAACACTAAGTAATCTGTGTAAATCCATTGATCCTAAGGTGGAAGCAAATCCCTTCTTGATAATATCATATGTTATGAGGGCTGCTGCTAAATTATCTGGATTTATTGCTAGTTCAGAGCGAGAGCTCTGGATTAGGAGGCAAAATCCATACCCTCCAGAAATTATAGAGTTATCATCTATCCAGCAAAATCCAAACAATTCTGAAATTATAGTCTCACCATCTAACCGGCAATCAGGCTCTCCTGAAATCCTTATGGTTGAAGCCATAAATATACCGTGTTCACTAGAGAAGACTGTTGAAAGAGTAATTCTTAATCTAACATCTTCTGGTTATTTCCTGGATGTTATTGCAAAGCAACTTGGGTTGTTGGAAGCTGCTGACCTTATGATCTCAAGGCCTGTAGGTAAGGCTTCCTCTGAGACAAGGCTGCACTTTGCTGCTCCAAAGCAATATCTGTTGGATATTTCTTCAGATCTTCTTCATGCCAAAGAAGTTCGTAGTTTTGAAGGATCTTTGAATGCAAGAGCAAGTTACTTGTCTGGTCAAGATATCTATGGTGATGCTATTTTGGCAACCATTGGCTACACATGGAGGTCCAAAGACCTTTTCTATGAAGATGTATCTGTGAAATCATATTACAGGATGCTTATTGGCAAGACACCAAGTGGACTTTACAGGCTGTCCAGAGGGGCAATACTTGCAGCTGAGTTACCTTTGGCATTTACTACAAATGCAAAATGGAAGGGTTCCTTACCAAGGGAGATGCTCTGTACATTCTGTCGTCAGCATCGGCTTTCTCAGCCTATGTTCTCTACTTTAAGCAGTCTAGAAGAATCATCTGACTCCTCACAATCACACAAGAAGTTAAGGGCGACAGACTTGGTTGCGAAAGACACACAACATGCAAATGGTTGTGTTGTTACTGCTGGTTCAAAGGAAACAGTGGAGTCCGGAGGTAGCTTTAGATGTGAAATAAAAGTATATTCTAAGTTTCAGGATTTGATTTTAGAATGCTCACCAAAAGAGTCTTTTAAGAAGCAGAGTGATTCTGTCCAGAATGCCTCTTTGAAAGTTCTCTTATGGTTAGATATATACTTTAGGGATCCAAATGTGCCTTTCGAGAGATTAAAGGCTTCTGCTGATGGACTTGACATTAGATTTGAACCGAAAATTTTTGTTGAAGCATTTATGCTTTGTCAATCTCTTCATAATGTCGGTCATAATGAGATTGAAGAAGGCAAATTAGTATATCCCAACTCTGTGAATGTGCCAAATGTTCTGTCTGGACACGGACTTCGTTCTCTGAATATAGAAGGCCCTGATTCTGGTATTTCTCCATCTAATGGGTCCTTATCATGTGTGAGTTATTCTGCATCTCTAGTGACTGAAGAGCATCTAAGAGAACAACTGGAAAGTAGTGATGACTTTGAGTTTGAGATAGCTTCTGGAGCAGTGAATCCACATCTTGAATCAGTTCTCATTCAGATGTCGGTCGATCAGTCTGCTTGTTTCATTGTGGATTTACCCCCTCGAGAGTTGATTTTTGCTGCAGCTGATGATTCTGCAAGGATGCTTTCCTTATTGTCTTCAAAAACCTGCTGCTTGGAGTACACCATAAGGTTGTTGCGGGTAACAGAACCCTTGGAGGACAGAATGGAGCAGGCCTTGTTCAGCCCTCCACTTTCAAAACAACGAGTGGAATATGCAGTGCAAAGCATCAAAGAATCTTGTGCTACTACTTTGGTTGACTTTGGATGTGGCTCTGGTAGTTTGTTGGATTCCTTATTAAATTATCCAACTTCTCTGGAAAAAATTGCTGGTGTTGATCTTTCACAAAAGAGTCTTACCCGTGCGGCAAAGATACTTAATTCCAAATTAAACTCGACTTCAGGTGCTGATACCTCATGTACACCCCTAAAATCTGCAATTCTTTATGATGGTTCGGTTACAGATCATGATTCTCGATTGTGTGGGTTTGATATAGGCACTTGCTTAGAGGTAATCGAGCATATGGAGGAAGATCAAGCACATCTCTTTGGCAATGTGGCATTGAGTTATTTCCGTCCTAAAATTCTCATTGTCTCGACACCAAACTATGAATATAATGTCATACTCCAGAAGTCTACTTTATCAACCACAGAAGATGATGTTGATGAAAGAAATGAATCACAACCTTGTAAGTTTCGTAACCATGACCACAAGTTTGAGTGGACGAGAGCGCAGTTCAATTGCTGGGCAACCGAATTGGCCACGAGGCACAACTACAGTGTTGAGTTCAGTGGGGTTGGTGGATCTGGTGATGAACCAGGTTTTGCTTCCCAGATTGCTGTATTCAGACGGAGAGCCATACCTCAGGAAGGTTATCCTGAACTCTCGGATTTGGAACATCGTTACCAAGTCATATGGGAATGGACTAGTAGCGATAGGTCAAGATGTTATGAAATCCTACGTAAATGGTTTTCCTGTTTCGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001510 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006379 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009653 GO:0009791 GO:0009892 GO:0009908 GO:0009909 GO:0009954 GO:0009987 GO:0010033 GO:0010051 GO:0010093 GO:0010305 GO:0010467 GO:0010468 GO:0010589 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016740 GO:0016741 GO:0019222 GO:0022414 GO:0031047 GO:0031050 GO:0032259 GO:0032501 GO:0032502 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035279 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048519 GO:0048580 GO:0048608 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0098542 GO:0098586 GO:0098795 GO:0099402 GO:0140098 GO:1901360 GO:1901698 GO:1901699 GO:1905392 GO:1905393 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKBP_C Methyltransf_11 Methyltransf_12 Methyltransf_31 dsrm

Protein Analysis

981

Amino Acids

108.77

Weight (kDa)

5.66

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSRM_2 PF24995 23 - 93 1e-12 dsRNA binding domain
Hen1_LCD PF21224 105 - 208 5.8e-44 Small RNA 2'-O-methyltransferase-like, La-motif-containing domain
Hen1_Lam_C PF18441 242 - 376 2.6e-59 Hen1 La-motif C-terminal domain
dsRBD2 PF17842 378 - 523 1.1e-59 Double-stranded RNA binding domain 2
Methyltransf_12 PF08242 739 - 839 7.5e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 278
Acc36I ACCTGC 1 cut(s) 2075
Acc65I GGTACC 1 cut(s) 80
AccB1I GGYRCC 1 cut(s) 80
AccB7I CCANNNNNTGG 1 cut(s) 1814
AccI GTMKAC 1 cut(s) 2594
AccIII TCCGGA 1 cut(s) 1421
AciI CCGC 5 cut(s) 175, 288, 383, 2098, 2324
AclWI GGATC 6 cut(s) 433, 494, 988, 1568, 1581, 2770
AcoI YGGCCR 1 cut(s) 2718
AcsI RAATTY 2 cut(s) 1641, 2541
AcuI CTGAAG 4 cut(s) 30, 924, 1878, 2343
AdeI CACNNNGTG 1 cut(s) 1136
AfaI GTAC 6 cut(s) 49, 82, 181, 1243, 2081, 2380
AfiI CCNNNNNNNGG 5 cut(s) 389, 1227, 1796, 1814, 2828
AhlI ACTAGT 1 cut(s) 2885
AjnI CCWGG 3 cut(s) 136, 807, 2776
AjuI GAANNNNNNNTTGG 2 cut(s) 1061, 1093
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 4 cut(s) 337, 688, 884, 2557
AlwI GGATC 6 cut(s) 433, 494, 988, 1568, 1581, 2770
AlwNI CAGNNNCTG 1 cut(s) 2366
Ama87I CYCGRG 2 cut(s) 336, 2001
Aor13HI TCCGGA 1 cut(s) 1421
AoxI GGCC 6 cut(s) 160, 261, 866, 1786, 2133, 2718
ApoI RAATTY 2 cut(s) 1641, 2541
Asp700I GAANNNNTTC 1 cut(s) 1944
Asp718I GGTACC 1 cut(s) 80
AspLEI GCGC 2 cut(s) 279, 2692
AspS9I GGNCC 4 cut(s) 161, 1065, 1787, 1818
AsuHPI GGTGA 5 cut(s) 274, 678, 1040, 1479, 2780
AsuII TTCGAA 1 cut(s) 34
AvaI CYCGRG 2 cut(s) 336, 2001
AvaII GGWCC 2 cut(s) 1065, 1818
AxyI CCTNAGG 3 cut(s) 54, 504, 2823
BaeI ACNNNNGTAYC 4 cut(s) 163, 192, 196, 225
BalI TGGCCA 1 cut(s) 2720
BamHI GGATCC 1 cut(s) 1573
BanI GGYRCC 1 cut(s) 80
BanII GRGCYC 1 cut(s) 597
BauI CACGAG 1 cut(s) 2722
BbsI GAAGAC 2 cut(s) 767, 2049
Bbv12I GWGCWC 1 cut(s) 597
BccI CCATC 5 cut(s) 317, 697, 1607, 1816, 2402
BceAI ACGGC 1 cut(s) 443
BcgI CGANNNNNNTGC 2 cut(s) 1955, 1989
BciT130I CCWGG 3 cut(s) 138, 809, 2778
BcoDI GTCTC 4 cut(s) 337, 688, 884, 2557
BcuI ACTAGT 1 cut(s) 2885
BfaI CTAG 6 cut(s) 42, 579, 755, 1298, 1850, 2886
BfmI CTRYAG 4 cut(s) 453, 870, 2019, 2734
BfuAI ACCTGC 1 cut(s) 2075
BglII AGATCT 1 cut(s) 943
BlpI GCTNAGC 1 cut(s) 240
Bme1390I CCNGG 3 cut(s) 138, 809, 2778
Bme18I GGWCC 2 cut(s) 1065, 1818
BmeT110I CYCGRG 2 cut(s) 336, 2001
BmgT120I GGNCC 4 cut(s) 161, 1065, 1787, 1818
BmiI GGNNCC 5 cut(s) 16, 82, 1219, 1575, 1819
BmrFI CCNGG 3 cut(s) 138, 809, 2778
BpiI GAAGAC 2 cut(s) 767, 2049
BpmI CTGGAG 3 cut(s) 609, 1938, 2570
Bpu1102I GCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 847
BsaAI YACGTR 1 cut(s) 2921
BsaJI CCNNGG 2 cut(s) 1226, 2112
BsaWI WCCGGW 1 cut(s) 1421
Bsc4I CCNNNNNNNGG 5 cut(s) 389, 1227, 1796, 1814, 2828
Bse118I RCCGGY 1 cut(s) 696
Bse1I ACTGG 1 cut(s) 1884
Bse21I CCTNAGG 3 cut(s) 54, 504, 2823
Bse3DI GCAATG 1 cut(s) 2518
BseAI TCCGGA 1 cut(s) 1421
BseBI CCWGG 3 cut(s) 138, 809, 2778
BseDI CCNNGG 2 cut(s) 1226, 2112
BseGI GGATG 8 cut(s) 137, 241, 328, 379, 817, 1119, 2046, 2231
BseLI CCNNNNNNNGG 5 cut(s) 389, 1227, 1796, 1814, 2828
BseMI GCAATG 1 cut(s) 2518
BseMII CTCAG 6 cut(s) 231, 377, 879, 1167, 1283, 2837
BseNI ACTGG 1 cut(s) 1884
BseRI GAGGAG 1 cut(s) 1306
BseYI CCCAGC 1 cut(s) 2703
BsgI GTGCAG 2 cut(s) 173, 884
Bsh1285I CGRYCG 1 cut(s) 1965
BshFI GGCC 6 cut(s) 162, 263, 868, 1788, 2135, 2720
BshNI GGYRCC 1 cut(s) 80
BsiEI CGRYCG 1 cut(s) 1965
BsiHKAI GWGCWC 1 cut(s) 597
BsiHKCI CYCGRG 2 cut(s) 336, 2001
BsiSI CCGG 2 cut(s) 697, 1422
BslI CCNNNNNNNGG 5 cut(s) 389, 1227, 1796, 1814, 2828
BsmAI GTCTC 4 cut(s) 337, 688, 884, 2557
BsmI GAATGC 3 cut(s) 994, 1487, 1534
BsnI GGCC 6 cut(s) 162, 263, 868, 1788, 2135, 2720
BsoBI CYCGRG 2 cut(s) 336, 2001
Bsp119I TTCGAA 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 597
Bsp13I TCCGGA 1 cut(s) 1421
Bsp1407I TGTACA 3 cut(s) 47, 1241, 2378
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 5 cut(s) 175, 288, 383, 2098, 2324
BspANI GGCC 6 cut(s) 162, 263, 868, 1788, 2135, 2720
BspCNI CTCAG 6 cut(s) 232, 378, 880, 1168, 1282, 2836
BspEI TCCGGA 1 cut(s) 1421
BspHI TCATGA 1 cut(s) 2425
BspLI GGNNCC 5 cut(s) 16, 82, 1219, 1575, 1819
BspMAI CTGCAG 1 cut(s) 2023
BspMI ACCTGC 1 cut(s) 2075
BspPI GGATC 6 cut(s) 433, 494, 988, 1568, 1581, 2770
BspQI GCTCTTC 1 cut(s) 1854
BspT104I TTCGAA 1 cut(s) 34
BspT107I GGYRCC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 2518
BsrFI RCCGGY 1 cut(s) 696
BsrGI TGTACA 3 cut(s) 47, 1241, 2378
BsrI ACTGG 1 cut(s) 1884
BssAI RCCGGY 1 cut(s) 696
BssECI CCNNGG 2 cut(s) 1226, 2112
BssSI CACGAG 1 cut(s) 2722
BssT1I CCWWGG 2 cut(s) 1226, 2112
Bst2BI CACGAG 1 cut(s) 2722
Bst2UI CCWGG 3 cut(s) 138, 809, 2778
Bst4CI ACNGT 6 cut(s) 31, 205, 747, 766, 1414, 2738
Bst6I CTCTTC 5 cut(s) 312, 354, 381, 1680, 1854
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstAUI TGTACA 3 cut(s) 47, 1241, 2378
BstBAI YACGTR 1 cut(s) 2921
BstBI TTCGAA 1 cut(s) 34
BstC8I GCNNGC 3 cut(s) 91, 433, 2133
BstENI CCTNNNNNAGG 2 cut(s) 1225, 2826
BstF5I GGATG 8 cut(s) 137, 241, 328, 379, 817, 1119, 2046, 2231
BstHHI GCGC 2 cut(s) 279, 2692
BstMAI GTCTC 4 cut(s) 337, 688, 884, 2557
BstMCI CGRYCG 1 cut(s) 1965
BstMWI GCNNNNNNNGC 6 cut(s) 174, 260, 1040, 1169, 1270, 1919
BstNI CCWGG 3 cut(s) 138, 809, 2778
BstNSI RCATGY 1 cut(s) 1376
BstSCI CCNGG 3 cut(s) 136, 807, 2776
BstSFI CTRYAG 4 cut(s) 453, 870, 2019, 2734
BstSNI TACGTA 1 cut(s) 2921
BstV2I GAAGAC 2 cut(s) 767, 2049
BstX2I RGATCY 4 cut(s) 943, 980, 1573, 2762
BstXI CCANNNNNNTGG 2 cut(s) 77, 318
BstYI RGATCY 4 cut(s) 943, 980, 1573, 2762
Bsu36I CCTNAGG 3 cut(s) 54, 504, 2823
BsuRI GGCC 6 cut(s) 162, 263, 868, 1788, 2135, 2720
BtsCI GGATG 8 cut(s) 137, 241, 328, 379, 817, 1119, 2046, 2231
BtsI GCAGTG 2 cut(s) 1929, 2184
BtsIMutI CAGTG 5 cut(s) 1419, 1929, 2184, 2743, 2755
BveI ACCTGC 1 cut(s) 2075
Cac8I GCNNGC 3 cut(s) 91, 433, 2133
CaiI CAGNNNCTG 1 cut(s) 2366
CciI TCATGA 1 cut(s) 2425
CfoI GCGC 2 cut(s) 279, 2692
Cfr10I RCCGGY 1 cut(s) 696
Cfr13I GGNCC 4 cut(s) 161, 1065, 1787, 1818
CsiI ACCWGGT 1 cut(s) 2776
Csp6I GTAC 6 cut(s) 48, 81, 180, 1242, 2080, 2379
CviQI GTAC 6 cut(s) 48, 81, 180, 1242, 2080, 2379
DraI TTTAAA 1 cut(s) 223
DraIII CACNNNGTG 1 cut(s) 1136
EaeI YGGCCR 1 cut(s) 2718
Eam1104I CTCTTC 5 cut(s) 312, 354, 381, 1680, 1854
EarI CTCTTC 5 cut(s) 312, 354, 381, 1680, 1854
Ecl136II GAGCTC 1 cut(s) 595
Eco105I TACGTA 1 cut(s) 2921
Eco130I CCWWGG 2 cut(s) 1226, 2112
Eco147I AGGCCT 2 cut(s) 868, 2135
Eco24I GRGCYC 1 cut(s) 597
Eco32I GATATC 1 cut(s) 1021
Eco47I GGWCC 2 cut(s) 1065, 1818
Eco53kI GAGCTC 1 cut(s) 595
Eco57I CTGAAG 4 cut(s) 30, 924, 1878, 2343
Eco81I CCTNAGG 3 cut(s) 54, 504, 2823
Eco88I CYCGRG 2 cut(s) 336, 2001
EcoICRI GAGCTC 1 cut(s) 595
EcoNI CCTNNNNNAGG 2 cut(s) 1225, 2826
EcoO109I RGGNCCY 3 cut(s) 161, 1787, 1818
EcoRII CCWGG 3 cut(s) 136, 807, 2776
EcoRV GATATC 1 cut(s) 1021
EcoT14I CCWWGG 2 cut(s) 1226, 2112
EcoT38I GRGCYC 1 cut(s) 597
ErhI CCWWGG 2 cut(s) 1226, 2112
FalI AAGNNNNNCTT 6 cut(s) 351, 383, 411, 443, 1205, 1237
FauI CCCGC 2 cut(s) 295, 2091
FauNDI CATATG 3 cut(s) 538, 2481, 2873
FblI GTMKAC 1 cut(s) 2594
FokI GGATG 8 cut(s) 144, 248, 335, 366, 824, 1126, 2053, 2238
FriOI GRGCYC 1 cut(s) 597
FspBI CTAG 6 cut(s) 42, 579, 755, 1298, 1850, 2886
FspI TGCGCA 1 cut(s) 278
GlaI GCGC 2 cut(s) 278, 2691
GsaI CCCAGC 1 cut(s) 2707
GsuI CTGGAG 3 cut(s) 609, 1938, 2570
HaeIII GGCC 6 cut(s) 162, 263, 868, 1788, 2135, 2720
HapII CCGG 2 cut(s) 697, 1422
HhaI GCGC 2 cut(s) 279, 2692
Hin6I GCGC 2 cut(s) 277, 2690
HinP1I GCGC 2 cut(s) 277, 2690
HincII GTYRAC 1 cut(s) 2218
HindII GTYRAC 1 cut(s) 2218
HpaII CCGG 2 cut(s) 697, 1422
HphI GGTGA 5 cut(s) 274, 678, 1040, 1479, 2780
Hpy166II GTNNAC 8 cut(s) 50, 752, 1139, 2082, 2218, 2381, 2595, 2682
Hpy8I GTNNAC 8 cut(s) 50, 752, 1139, 2082, 2218, 2381, 2595, 2682
HpyAV CCTTC 9 cut(s) 5, 226, 413, 532, 971, 1207, 1700, 1778, 2822
HpyCH4III ACNGT 6 cut(s) 31, 205, 747, 766, 1414, 2738
HpyCH4IV ACGT 1 cut(s) 2920
HpyF10VI GCNNNNNNNGC 6 cut(s) 174, 260, 1040, 1169, 1270, 1919
HpySE526I ACGT 1 cut(s) 2920
HspAI GCGC 2 cut(s) 277, 2690
Kpn2I TCCGGA 1 cut(s) 1421
KpnI GGTACC 1 cut(s) 84
LguI GCTCTTC 1 cut(s) 1854
LmnI GCTCC 4 cut(s) 14, 916, 1919, 2128
MabI ACCWGGT 1 cut(s) 2776
MaeI CTAG 6 cut(s) 42, 579, 755, 1298, 1850, 2886
MaeII ACGT 1 cut(s) 2920
MaeIII GTNAC 9 cut(s) 25, 1001, 1179, 1390, 1852, 2101, 2416, 2657, 2861
MfeI CAATTG 1 cut(s) 2698
MflI RGATCY 4 cut(s) 943, 980, 1573, 2762
MhlI GDGCHC 1 cut(s) 597
MlsI TGGCCA 1 cut(s) 2720
MluNI TGGCCA 1 cut(s) 2720
MlyI GAGTC 5 cut(s) 125, 1307, 1427, 1505, 2320
MmeI TCCRAC 4 cut(s) 819, 909, 2226, 2291
Mox20I TGGCCA 1 cut(s) 2720
MroI TCCGGA 1 cut(s) 1421
MroXI GAANNNNTTC 1 cut(s) 1944
MscI TGGCCA 1 cut(s) 2720
MseI TTAA 9 cut(s) 222, 783, 1289, 1337, 1503, 1598, 2258, 2337, 2348
MslI CAYNNNNRTG 2 cut(s) 1377, 1955
Msp20I TGGCCA 1 cut(s) 2720
MspA1I CMGCKG 4 cut(s) 177, 385, 1175, 2024
MspI CCGG 2 cut(s) 697, 1422
MspR9I CCNGG 3 cut(s) 138, 809, 2778
MunI CAATTG 1 cut(s) 2698
Mva1269I GAATGC 3 cut(s) 994, 1487, 1534
MvaI CCWGG 3 cut(s) 138, 809, 2778
MwoI GCNNNNNNNGC 6 cut(s) 174, 260, 1040, 1169, 1270, 1919
NdeI CATATG 3 cut(s) 538, 2481, 2873
NlaIV GGNNCC 5 cut(s) 16, 82, 1219, 1575, 1819
NmuCI GTSAC 2 cut(s) 25, 1852
NsbI TGCGCA 1 cut(s) 278
NspI RCATGY 1 cut(s) 1376
NspV TTCGAA 1 cut(s) 34
PaeR7I CTCGAG 1 cut(s) 2001
PagI TCATGA 1 cut(s) 2425
PceI AGGCCT 2 cut(s) 868, 2135
PciSI GCTCTTC 1 cut(s) 1854
PctI GAATGC 3 cut(s) 994, 1487, 1534
PdmI GAANNNNTTC 1 cut(s) 1944
PflMI CCANNNNNTGG 1 cut(s) 1814
PfoI TCCNGGA 2 cut(s) 136, 807
PleI GAGTC 5 cut(s) 124, 1307, 1426, 1504, 2319
PpsI GAGTC 5 cut(s) 124, 1307, 1426, 1504, 2319
Ppu21I YACGTR 1 cut(s) 2921
PpuMI RGGWCCY 1 cut(s) 1818
Psp124BI GAGCTC 1 cut(s) 597
Psp5II RGGWCCY 1 cut(s) 1818
Psp6I CCWGG 3 cut(s) 136, 807, 2776
PspFI CCCAGC 1 cut(s) 2703
PspGI CCWGG 3 cut(s) 136, 807, 2776
PspN4I GGNNCC 5 cut(s) 16, 82, 1219, 1575, 1819
PspPI GGNCC 4 cut(s) 161, 1065, 1787, 1818
PspPPI RGGWCCY 1 cut(s) 1818
PstI CTGCAG 1 cut(s) 2023
PstNI CAGNNNCTG 1 cut(s) 2366
PsuI RGATCY 4 cut(s) 943, 980, 1573, 2762
PvuII CAGCTG 2 cut(s) 1175, 2024
RsaI GTAC 6 cut(s) 49, 82, 181, 1243, 2081, 2380
RsaNI GTAC 6 cut(s) 48, 81, 180, 1242, 2080, 2379
RseI CAYNNNNRTG 2 cut(s) 1377, 1955
SacI GAGCTC 1 cut(s) 597
SapI GCTCTTC 1 cut(s) 1854
SaqAI TTAA 9 cut(s) 222, 783, 1289, 1337, 1503, 1598, 2258, 2337, 2348
Sau96I GGNCC 4 cut(s) 161, 1065, 1787, 1818
SchI GAGTC 5 cut(s) 125, 1307, 1427, 1505, 2320
ScrFI CCNGG 3 cut(s) 138, 809, 2778
SduI GDGCHC 1 cut(s) 597
SexAI ACCWGGT 1 cut(s) 2776
SfcI CTRYAG 4 cut(s) 453, 870, 2019, 2734
Sfr274I CTCGAG 1 cut(s) 2001
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 2 cut(s) 1065, 1818
SlaI CTCGAG 1 cut(s) 2001
SmiMI CAYNNNNRTG 2 cut(s) 1377, 1955
SmlI CTYRAG 2 cut(s) 862, 2001
SmoI CTYRAG 2 cut(s) 862, 2001
SnaBI TACGTA 1 cut(s) 2921
SpeI ACTAGT 1 cut(s) 2885
SseBI AGGCCT 2 cut(s) 868, 2135
SsiI CCGC 5 cut(s) 175, 288, 383, 2098, 2324
SspI AATATT 1 cut(s) 347
SspMI CTAG 6 cut(s) 42, 579, 755, 1298, 1850, 2886
SstI GAGCTC 1 cut(s) 597
StuI AGGCCT 2 cut(s) 868, 2135
StyD4I CCNGG 3 cut(s) 136, 807, 2776
StyI CCWWGG 2 cut(s) 1226, 2112
TaaI ACNGT 6 cut(s) 31, 205, 747, 766, 1414, 2738
TaiI ACGT 1 cut(s) 2923
TaqI TCGA 8 cut(s) 34, 1590, 1965, 2002, 2354, 2434, 2475, 2555
TaqII GACCGA 2 cut(s) 1676, 1951
TatI WGTACW 4 cut(s) 47, 1241, 2079, 2378
TauI GCSGC 1 cut(s) 2327
Tru1I TTAA 9 cut(s) 222, 783, 1289, 1337, 1503, 1598, 2258, 2337, 2348
Tru9I TTAA 9 cut(s) 222, 783, 1289, 1337, 1503, 1598, 2258, 2337, 2348
TscAI CASTG 5 cut(s) 1419, 1929, 2184, 2743, 2755
TseFI GTSAC 2 cut(s) 25, 1852
Tsp45I GTSAC 2 cut(s) 25, 1852
TspGWI ACGGA 3 cut(s) 1776, 2522, 2824
TspRI CASTG 5 cut(s) 1419, 1929, 2184, 2743, 2755
Van91I CCANNNNNTGG 1 cut(s) 1814
VpaK11BI GGWCC 2 cut(s) 1065, 1818
XagI CCTNNNNNAGG 2 cut(s) 1225, 2826
XapI RAATTY 2 cut(s) 1641, 2541
XbaI TCTAGA 1 cut(s) 1297
XceI RCATGY 1 cut(s) 1376
XhoI CTCGAG 1 cut(s) 2001
XmiI GTMKAC 1 cut(s) 2594
XmnI GAANNNNTTC 1 cut(s) 1944
XspI CTAG 6 cut(s) 42, 579, 755, 1298, 1850, 2886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.