MD10G1252200.v1.1

Small RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002993
Physical Location & Seq
Reverse (-)
34508277 .. 34514554
6278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1252200.v1.1.491

Sequence Viewer

Length: 2865 bp
ATGGAAACTGAAGGAGCTGCAACTGTTACAATGCGAAACAATACTGATACACCTAAGGCTATTATACACAAAAAATTCGGTAGCATGGCCTGCTATAAAGTAGAGGAAGTGCATGAGCCTCCTCAAAATGGATGTCCACGGTTGGCCATTATGCAGAAGGGCCCTTGCCTTTATCGCTGTACCTTGCAACTTCCAGAAGTTACTGTCGTATCTGGAACCTTTAAAAAGAAGAAGGACGCAGAGCAATCTGCCGCTGAATTGGCCTTAGAAAAGTTAGGCGTTGATCCAACAAAAGTCCCTACTCTGCAGGAAGCATGGGATAATTTAGTCGCTCATGTCAGTTTCTTATTTTCAGACGAGTTTCTTCCAGCTCTTCATCCTCTGAGTGGTCACTTCAGAGCAGCTTTGCAGAGAGAAGGTGACCTTTCAGGTCAGATTCCTGCTTCTGTCATTGCCGTCTTTGATGCAAGACTTTTTAATATGTGTAAATCCATTGATCCTGAGGTGGAGTCAAATCCTTTCTTGGTTATATCATATGTTATGAGGGCTGCTGCTAGATTATCTGGACTGCTTGCTACTTGTGAGGAAGAGCTCTGGATTAGGAGGAAAAATCCATACACTCCTGAAATAATAGAGTCATCATCTGTGGAGCAATCAGACTCCAGAGAAAACTTTTCAATTGAAGCCATAAATGTACCATGTTCTTTAGAAAAGAGGGTTGAAAAAGTAATCCTTAATCTCTCATCTTCTGGGTATTTCCTGGATGTTATTGCTAAACAACTTGGGTTATTGAATGCTGCTGACATTCTGATCTCAAGGCCTATAGGTAAGGCTTCCTCTGAGACAAGACTGTACTTTGCTGCTCCCAAGAAATATCTGTTGGATATTTCTTCTGATCTTCTACATGCCAAGGGAGCTTGCAACTCCAAAGGATCTTTCAATGCAAGAGCAAGTTCCTTGTCTGGCCAAGATATATATGGTGACGCTGTTTTGGCATCCATTGGATTCACGCAGAGGTCCAAAGACCTTTTCTATGAAGATGTTTCCCTGCAATCATATCACAGGATGGTCATTGGCAAGACACCAAGTGGAATTTACAAGTTGTCCAGAGAAGGAATACTTACAGCTGAGTTACCTTTGGCATTTACTGCCAAAGCAAAATGGAAGGGTTCGATGCCATGGGATATACTATGTACATTCTGCCATCAGCACCGGCTTGAGCCCATGTTCACTCCTCGAAGCACTCTAGAGGAATCATCCGAGTCACCAATATCACACAAGAAGTTAAAGGTGACAGACTTGGCTGGGAAAGAGACACAATATGCAAATGGCTGTGTTGTTGATGCTGGCGTAAATGAGATAGTTGAACCCAGAGGTAGCTTTAGATGTGAAGTAAAAATAATTTCCAAGTTTGAGGATATTATTTTAGAGTGCTCACCAAAAGAGACTTTTAAGAAGCAGAGTGATGCTATCCAGAATGCTTCTTTGAAAGTTCTGTTATGGTTAAATGCATATTTTGGGGATCCAACTGTGCCTTTAGAGAGACTAAGTACTTCTGCTGATGGCCATAACATTAGATTCGATTCCCAAACCTTTATTAAAACATTTATGCTGTGCCAACATATTCATAATGTTGGGCATAATGAGACTGAAGATGGCAAATTAGCATATTCCAACTCTGTGAATGCGTCATTTGCTCTCCCTGGAATTGAACTTCGTTCTTTAAGTATAGAAGGACCGGATTCTGGTGTTTTTCCATCTATTGGGTCCTTAGCATGTGCGAATTATTCTGTTTCTTTGGTGACTGAAGGAAAACATATGAAAGAACTTTTGGAAAGTAGTGATGACTTTGAGTTTGAGATAGCATCTGGAGCAGTGATTCCACATCTCGAGTCAGTTATCAAACAAATGACTGTTGGTCAATCGGCTTTTTTCAGTATGGATTTGCCACCTCAAGAGTTGGTTTTTGCTGCAGCTGGCAATTCTGCTAGAATGCTTTCGTTATTGTCTTCAGAGACCTGCTATTTGGAGTACACTATAACTTTGTTGGGGGTAACCGAACCACTGGAGGATAGAATGGAGCAGGCTTTTTTCAGCCCTCCGCTTTCAAAACAGCGTGTGGAATATGCAGTGCAAAGCATCAAAAAATCTTGTGCAACTACTTTGGTTGACTTTGGATGTGGCTCTGGTAGTTTGTTGGATTCTTTACTAAACTATCCAACTTCTCTGGAAAAAATTGCTGGTGTTGATATTTCACAAAAGAGTCTTGCCCGTGCGGCAAAGATACTTCATTCCAAGTTAAACTCAAATTCAGATGCCAGTACGTCAGCCATGAAATCAGCAATTCTTTATGATGGTTCAATTACAAATTTTGATTCCCGGTTGAGTGGATTTGATAGTGGCACTTGCTTAGAGGTAATAGAGCATATGGAGGAAGATCAAGCATCTGAGTTTGGCAACGTGGTACTAAGTTTATTTTGTCCAAGGGTTCTTATTGTCTCTACTCCAAACTATGAATACAATGTGATACTCCAGAAATCCAATTTGTCAAGCCAAGAAGATGACCCAGAGGACAAAAGTCAAGCACAATGTAAATTTCGTAACCATGACCACAAGTTCGAGTGGACAAGAGAGCAGTTCAATTGCTGGGCAACTGAATTGGCTGCGAGGCACAATTACAGTGTTGAGTTCAGTGGAGTTGGAGGTTCCGGTGATACAGAACCAGGTTTTGCTTCCCAGATTGCTGTCTTTAGACATGAACTCGTAACTAAAGAAGTCGCTTTTCCAGAGGTTTCAGGTTTGGAACAACCTTACCAAGCTATATGGGAATGGAACAGCAGAGATAGGCCAAGGTCTGCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001510 GO:0002252 GO:0002376 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006379 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0009451 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009653 GO:0009791 GO:0009892 GO:0009908 GO:0009909 GO:0009954 GO:0009987 GO:0010033 GO:0010051 GO:0010093 GO:0010305 GO:0010467 GO:0010468 GO:0010589 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016740 GO:0016741 GO:0019222 GO:0022414 GO:0031047 GO:0031050 GO:0032259 GO:0032501 GO:0032502 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035279 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0046483 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048519 GO:0048580 GO:0048608 GO:0048646 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0098542 GO:0098586 GO:0098795 GO:0099402 GO:0140098 GO:1901360 GO:1901698 GO:1901699 GO:1905392 GO:1905393 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKBP_C Methyltransf_11 Methyltransf_12 Methyltransf_31 dsrm

Protein Analysis

955

Amino Acids

105.27

Weight (kDa)

5.37

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSRM_2 PF24995 23 - 93 8e-12 dsRNA binding domain
Hen1_LCD PF21224 104 - 206 3.4e-44 Small RNA 2'-O-methyltransferase-like, La-motif-containing domain
Hen1_Lam_C PF18441 226 - 360 4.8e-57 Hen1 La-motif C-terminal domain
dsRBD2 PF17842 362 - 506 2.3e-56 Double-stranded RNA binding domain 2
Methyltransf_12 PF08242 723 - 816 5.4e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2027
AccB7I CCANNNNNTGG 1 cut(s) 1763
AciI CCGC 3 cut(s) 252, 2102, 2276
AclWI GGATC 5 cut(s) 278, 491, 940, 1517, 1530
AcoI YGGCCR 3 cut(s) 144, 964, 1564
AcsI RAATTY 5 cut(s) 74, 1092, 2308, 2368, 2594
AcuI CTGAAG 5 cut(s) 30, 379, 1671, 1827, 1995
AdeI CACNNNGTG 1 cut(s) 1088
AfaI GTAC 8 cut(s) 181, 696, 854, 1195, 1552, 2033, 2323, 2466
AfiI CCNNNNNNNGG 4 cut(s) 128, 386, 1745, 1763
AhdI GACNNNNNGTC 1 cut(s) 1917
AjnI CCWGG 3 cut(s) 759, 1702, 2722
AjuI GAANNNNNNNTTGG 8 cut(s) 863, 895, 920, 952, 1013, 1045, 1814, 1846
AluBI AGCT 9 cut(s) 17, 371, 404, 592, 917, 1127, 1380, 1976, 2819
AluI AGCT 9 cut(s) 17, 371, 404, 592, 917, 1127, 1380, 1976, 2819
Alw21I GWGCWC 2 cut(s) 594, 1436
Alw26I GTCTC 7 cut(s) 836, 1307, 1439, 1537, 1640, 2009, 2503
AlwI GGATC 5 cut(s) 278, 491, 940, 1517, 1530
Ama87I CYCGRG 1 cut(s) 1889
AoxI GGCC 8 cut(s) 87, 144, 160, 261, 818, 964, 1564, 2846
ApaI GGGCCC 1 cut(s) 164
ApeKI GCWGC 9 cut(s) 17, 401, 548, 551, 797, 860, 1970, 1973, 2663
ApoI RAATTY 5 cut(s) 74, 1092, 2308, 2368, 2594
ArsI GACNNNNNNTTYG 8 cut(s) 118, 150, 1838, 1870, 2008, 2040, 2600, 2632
Asp700I GAANNNNTTC 1 cut(s) 1996
AspS9I GGNCC 5 cut(s) 160, 161, 1017, 1736, 1767
AsuC2I CCSGG 1 cut(s) 2380
AsuHPI GGTGA 7 cut(s) 431, 992, 1257, 1303, 1428, 1813, 2723
AvaI CYCGRG 1 cut(s) 1889
AvaII GGWCC 3 cut(s) 1017, 1736, 1767
AxyI CCTNAGG 2 cut(s) 54, 501
BaeGI GKGCMC 1 cut(s) 164
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BalI TGGCCA 3 cut(s) 146, 966, 1566
BamHI GGATCC 1 cut(s) 1522
BanII GRGCYC 3 cut(s) 164, 594, 1224
BbsI GAAGAC 1 cut(s) 2001
Bbv12I GWGCWC 2 cut(s) 594, 1436
BbvI GCAGC 9 cut(s) 4, 413, 535, 538, 784, 847, 1957, 1985, 2650
BccI CCATC 6 cut(s) 1060, 1212, 1556, 1649, 1765, 2348
BceAI ACGGC 1 cut(s) 440
BciT130I CCWGG 3 cut(s) 761, 1704, 2724
BcnI CCSGG 1 cut(s) 2380
BcoDI GTCTC 7 cut(s) 836, 1307, 1439, 1537, 1640, 2009, 2503
BfaI CTAG 3 cut(s) 555, 1247, 1989
BfmI CTRYAG 3 cut(s) 305, 822, 1971
BfuAI ACCTGC 1 cut(s) 2027
BglI GCCNNNNNGGC 1 cut(s) 2276
BmcAI AGTACT 1 cut(s) 1552
Bme1390I CCNGG 4 cut(s) 761, 1704, 2380, 2724
Bme18I GGWCC 3 cut(s) 1017, 1736, 1767
BmeRI GACNNNNNGTC 1 cut(s) 1917
BmeT110I CYCGRG 1 cut(s) 1889
BmgT120I GGNCC 5 cut(s) 160, 161, 1017, 1736, 1767
BmiI GGNNCC 5 cut(s) 162, 217, 1524, 1768, 2707
BmrFI CCNGG 4 cut(s) 761, 1704, 2380, 2724
BmsI GCATC 9 cut(s) 454, 1004, 1164, 1333, 1456, 1874, 2148, 2305, 2453
BoxI GACNNNNGTC 1 cut(s) 2577
BpiI GAAGAC 1 cut(s) 2001
BpmI CTGGAG 4 cut(s) 646, 1890, 2087, 2516
Bpu10I CCTNAGC 1 cut(s) 1771
BpuEI CTTGAG 3 cut(s) 799, 1238, 1938
BpuMI CCSGG 1 cut(s) 2380
BsaI GGTCTC 1 cut(s) 2009
BsaJI CCNNGG 6 cut(s) 137, 909, 1178, 1702, 2483, 2849
BsaWI WCCGGW 2 cut(s) 1738, 2708
Bsc4I CCNNNNNNNGG 4 cut(s) 128, 386, 1745, 1763
Bse118I RCCGGY 1 cut(s) 1212
Bse1I ACTGG 2 cut(s) 2070, 2319
Bse21I CCTNAGG 2 cut(s) 54, 501
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 3 cut(s) 761, 1704, 2724
BseDI CCNNGG 6 cut(s) 137, 909, 1178, 1702, 2483, 2849
BseGI GGATG 7 cut(s) 137, 376, 769, 995, 1071, 1256, 2183
BseLI CCNNNNNNNGG 4 cut(s) 128, 386, 1745, 1763
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 5 cut(s) 374, 492, 831, 1119, 2439
BseNI ACTGG 2 cut(s) 2070, 2319
BseRI GAGGAG 2 cut(s) 111, 1224
BseSI GKGCMC 1 cut(s) 164
BseXI GCAGC 9 cut(s) 4, 413, 535, 538, 784, 847, 1957, 1985, 2650
BseYI CCCAGC 2 cut(s) 1304, 2646
BshFI GGCC 8 cut(s) 89, 146, 162, 263, 820, 966, 1566, 2848
BsiHKAI GWGCWC 2 cut(s) 594, 1436
BsiHKCI CYCGRG 1 cut(s) 1889
BsiSI CCGG 4 cut(s) 1213, 1739, 2380, 2709
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 4 cut(s) 128, 386, 1745, 1763
BsmAI GTCTC 7 cut(s) 836, 1307, 1439, 1537, 1640, 2009, 2503
BsmFI GGGAC 1 cut(s) 281
BsmI GAATGC 4 cut(s) 799, 1483, 1690, 1998
BsnI GGCC 8 cut(s) 89, 146, 162, 263, 820, 966, 1566, 2848
Bso31I GGTCTC 1 cut(s) 2009
BsoBI CYCGRG 1 cut(s) 1889
Bsp120I GGGCCC 1 cut(s) 160
Bsp1286I GDGCHC 4 cut(s) 164, 594, 1224, 1436
Bsp1407I TGTACA 1 cut(s) 1193
Bsp143I GATC 7 cut(s) 283, 496, 810, 895, 932, 1522, 2437
Bsp19I CCATGG 1 cut(s) 1178
BspACI CCGC 3 cut(s) 252, 2102, 2276
BspANI GGCC 8 cut(s) 89, 146, 162, 263, 820, 966, 1566, 2848
BspCNI CTCAG 5 cut(s) 375, 493, 832, 1120, 2440
BspLI GGNNCC 5 cut(s) 162, 217, 1524, 1768, 2707
BspMAI CTGCAG 2 cut(s) 309, 1975
BspMI ACCTGC 1 cut(s) 2027
BspPI GGATC 5 cut(s) 278, 491, 940, 1517, 1530
BspQI GCTCTTC 2 cut(s) 378, 582
BspTNI GGTCTC 1 cut(s) 2009
BsrDI GCAATG 1 cut(s) 450
BsrFI RCCGGY 1 cut(s) 1212
BsrGI TGTACA 1 cut(s) 1193
BsrI ACTGG 2 cut(s) 2070, 2319
BssAI RCCGGY 1 cut(s) 1212
BssECI CCNNGG 6 cut(s) 137, 909, 1178, 1702, 2483, 2849
BssMI GATC 7 cut(s) 283, 496, 810, 895, 932, 1522, 2437
BssT1I CCWWGG 4 cut(s) 909, 1178, 2483, 2849
Bst2UI CCWGG 3 cut(s) 761, 1704, 2724
Bst4CI ACNGT 7 cut(s) 25, 141, 205, 852, 1531, 1915, 2681
Bst6I CTCTTC 2 cut(s) 378, 582
BstAPI GCANNNNNTGC 2 cut(s) 90, 1148
BstAUI TGTACA 1 cut(s) 1193
BstC8I GCNNGC 6 cut(s) 91, 573, 919, 1348, 1978, 2085
BstDSI CCRYGG 2 cut(s) 137, 1178
BstEII GGTNACC 2 cut(s) 419, 2053
BstF5I GGATG 7 cut(s) 137, 376, 769, 995, 1071, 1256, 2183
BstKTI GATC 7 cut(s) 286, 499, 813, 898, 935, 1525, 2440
BstMAI GTCTC 7 cut(s) 836, 1307, 1439, 1537, 1640, 2009, 2503
BstMBI GATC 7 cut(s) 283, 496, 810, 895, 932, 1522, 2437
BstMWI GCNNNNNNNGC 9 cut(s) 90, 174, 260, 914, 992, 1148, 1694, 1871, 2276
BstNI CCWGG 3 cut(s) 761, 1704, 2724
BstNSI RCATGY 2 cut(s) 908, 1779
BstPAI GACNNNNGTC 1 cut(s) 2577
BstPI GGTNACC 2 cut(s) 419, 2053
BstSCI CCNGG 4 cut(s) 759, 1702, 2378, 2722
BstSFI CTRYAG 3 cut(s) 305, 822, 1971
BstSLI GKGCMC 1 cut(s) 164
BstV1I GCAGC 9 cut(s) 4, 413, 535, 538, 784, 847, 1957, 1985, 2650
BstV2I GAAGAC 1 cut(s) 2001
BstX2I RGATCY 2 cut(s) 932, 1522
BstYI RGATCY 2 cut(s) 932, 1522
Bsu36I CCTNAGG 2 cut(s) 54, 501
BsuRI GGCC 8 cut(s) 89, 146, 162, 263, 820, 966, 1566, 2848
BtgI CCRYGG 2 cut(s) 137, 1178
BtsCI GGATG 7 cut(s) 137, 376, 769, 995, 1071, 1256, 2183
BtsI GCAGTG 2 cut(s) 1881, 2136
BtsIMutI CAGTG 5 cut(s) 1881, 2063, 2136, 2686, 2698
BveI ACCTGC 1 cut(s) 2027
Cac8I GCNNGC 6 cut(s) 91, 573, 919, 1348, 1978, 2085
Cfr10I RCCGGY 1 cut(s) 1212
Cfr13I GGNCC 5 cut(s) 160, 161, 1017, 1736, 1767
CseI GACGC 3 cut(s) 245, 992, 1677
CsiI ACCWGGT 1 cut(s) 2722
Csp6I GTAC 8 cut(s) 180, 695, 853, 1194, 1551, 2032, 2322, 2465
CviQI GTAC 8 cut(s) 180, 695, 853, 1194, 1551, 2032, 2322, 2465
DpnI GATC 7 cut(s) 285, 498, 812, 897, 934, 1524, 2439
DpnII GATC 7 cut(s) 283, 496, 810, 895, 932, 1522, 2437
DraI TTTAAA 1 cut(s) 223
DraIII CACNNNGTG 1 cut(s) 1088
DriI GACNNNNNGTC 1 cut(s) 1917
EaeI YGGCCR 3 cut(s) 144, 964, 1564
Eam1104I CTCTTC 2 cut(s) 378, 582
Eam1105I GACNNNNNGTC 1 cut(s) 1917
EarI CTCTTC 2 cut(s) 378, 582
Ecl136II GAGCTC 1 cut(s) 592
Eco130I CCWWGG 4 cut(s) 909, 1178, 2483, 2849
Eco147I AGGCCT 1 cut(s) 820
Eco24I GRGCYC 3 cut(s) 164, 594, 1224
Eco31I GGTCTC 1 cut(s) 2009
Eco47I GGWCC 3 cut(s) 1017, 1736, 1767
Eco53kI GAGCTC 1 cut(s) 592
Eco57I CTGAAG 5 cut(s) 30, 379, 1671, 1827, 1995
Eco81I CCTNAGG 2 cut(s) 54, 501
Eco88I CYCGRG 1 cut(s) 1889
Eco91I GGTNACC 2 cut(s) 419, 2053
EcoICRI GAGCTC 1 cut(s) 592
EcoO109I RGGNCCY 3 cut(s) 160, 161, 1767
EcoO65I GGTNACC 2 cut(s) 419, 2053
EcoRII CCWGG 3 cut(s) 759, 1702, 2722
EcoT14I CCWWGG 4 cut(s) 909, 1178, 2483, 2849
EcoT22I ATGCAT 1 cut(s) 1513
EcoT38I GRGCYC 3 cut(s) 164, 594, 1224
ErhI CCWWGG 4 cut(s) 909, 1178, 2483, 2849
FalI AAGNNNNNCTT 6 cut(s) 408, 440, 717, 749, 1104, 1136
FaqI GGGAC 1 cut(s) 281
FauNDI CATATG 3 cut(s) 535, 1818, 2427
FokI GGATG 7 cut(s) 144, 363, 776, 982, 1078, 1243, 2190
FriOI GRGCYC 3 cut(s) 164, 594, 1224
FspBI CTAG 3 cut(s) 555, 1247, 1989
GsaI CCCAGC 2 cut(s) 1308, 2650
GsuI CTGGAG 4 cut(s) 646, 1890, 2087, 2516
HaeIII GGCC 8 cut(s) 89, 146, 162, 263, 820, 966, 1566, 2848
HapII CCGG 4 cut(s) 1213, 1739, 2380, 2709
HgaI GACGC 3 cut(s) 245, 992, 1677
HincII GTYRAC 1 cut(s) 2170
HindII GTYRAC 1 cut(s) 2170
HpaII CCGG 4 cut(s) 1213, 1739, 2380, 2709
HphI GGTGA 7 cut(s) 431, 992, 1257, 1303, 1428, 1813, 2723
Hpy166II GTNNAC 5 cut(s) 137, 1230, 2034, 2170, 2625
Hpy8I GTNNAC 5 cut(s) 137, 1230, 2034, 2170, 2625
HpyAV CCTTC 8 cut(s) 5, 151, 226, 410, 1106, 1159, 1727, 1802
HpyCH4III ACNGT 7 cut(s) 25, 141, 205, 852, 1531, 1915, 2681
HpyCH4IV ACGT 2 cut(s) 2324, 2460
HpyF10VI GCNNNNNNNGC 9 cut(s) 90, 174, 260, 914, 992, 1148, 1694, 1871, 2276
HpySE526I ACGT 2 cut(s) 2324, 2460
Kzo9I GATC 7 cut(s) 283, 496, 810, 895, 932, 1522, 2437
LguI GCTCTTC 2 cut(s) 378, 582
LmnI GCTCC 6 cut(s) 14, 649, 868, 914, 1871, 2080
Lsp1109I GCAGC 9 cut(s) 4, 413, 535, 538, 784, 847, 1957, 1985, 2650
LweI GCATC 9 cut(s) 454, 1004, 1164, 1333, 1456, 1874, 2148, 2305, 2453
MabI ACCWGGT 1 cut(s) 2722
MaeI CTAG 3 cut(s) 555, 1247, 1989
MaeII ACGT 2 cut(s) 2324, 2460
MalI GATC 7 cut(s) 285, 498, 812, 897, 934, 1524, 2439
MboI GATC 7 cut(s) 283, 496, 810, 895, 932, 1522, 2437
MfeI CAATTG 2 cut(s) 678, 2641
MflI RGATCY 2 cut(s) 932, 1522
MhlI GDGCHC 4 cut(s) 164, 594, 1224, 1436
MlsI TGGCCA 3 cut(s) 146, 966, 1566
MluNI TGGCCA 3 cut(s) 146, 966, 1566
MlyI GAGTC 6 cut(s) 518, 644, 653, 1271, 1901, 2272
MmeI TCCRAC 7 cut(s) 311, 861, 1550, 1698, 2178, 2243, 2680
Mox20I TGGCCA 3 cut(s) 146, 966, 1566
Mph1103I ATGCAT 1 cut(s) 1513
MroXI GAANNNNTTC 1 cut(s) 1996
MscI TGGCCA 3 cut(s) 146, 966, 1566
MseI TTAA 9 cut(s) 222, 477, 735, 1286, 1452, 1505, 1599, 1724, 2300
Msp20I TGGCCA 3 cut(s) 146, 966, 1566
MspA1I CMGCKG 3 cut(s) 254, 1127, 1976
MspI CCGG 4 cut(s) 1213, 1739, 2380, 2709
MspR9I CCNGG 4 cut(s) 761, 1704, 2380, 2724
MunI CAATTG 2 cut(s) 678, 2641
Mva1269I GAATGC 4 cut(s) 799, 1483, 1690, 1998
MvaI CCWGG 3 cut(s) 761, 1704, 2724
MwoI GCNNNNNNNGC 9 cut(s) 90, 174, 260, 914, 992, 1148, 1694, 1871, 2276
NciI CCSGG 1 cut(s) 2380
NcoI CCATGG 1 cut(s) 1178
NdeI CATATG 3 cut(s) 535, 1818, 2427
NdeII GATC 7 cut(s) 283, 496, 810, 895, 932, 1522, 2437
NlaIV GGNNCC 5 cut(s) 162, 217, 1524, 1768, 2707
NmuCI GTSAC 6 cut(s) 389, 419, 980, 1263, 1291, 1801
NsiI ATGCAT 1 cut(s) 1513
NspI RCATGY 2 cut(s) 908, 1779
PaeR7I CTCGAG 1 cut(s) 1889
PceI AGGCCT 1 cut(s) 820
PciSI GCTCTTC 2 cut(s) 378, 582
PctI GAATGC 4 cut(s) 799, 1483, 1690, 1998
PdmI GAANNNNTTC 1 cut(s) 1996
PfeI GAWTC 9 cut(s) 436, 1005, 1253, 1578, 1583, 1742, 1879, 2201, 2375
PflMI CCANNNNNTGG 1 cut(s) 1763
PfoI TCCNGGA 1 cut(s) 759
PleI GAGTC 6 cut(s) 517, 643, 653, 1270, 1900, 2271
PpsI GAGTC 6 cut(s) 517, 643, 653, 1270, 1900, 2271
PpuMI RGGWCCY 1 cut(s) 1767
PshAI GACNNNNGTC 1 cut(s) 2577
Psp124BI GAGCTC 1 cut(s) 594
Psp5II RGGWCCY 1 cut(s) 1767
Psp6I CCWGG 3 cut(s) 759, 1702, 2722
PspEI GGTNACC 2 cut(s) 419, 2053
PspFI CCCAGC 2 cut(s) 1304, 2646
PspGI CCWGG 3 cut(s) 759, 1702, 2722
PspN4I GGNNCC 5 cut(s) 162, 217, 1524, 1768, 2707
PspOMI GGGCCC 1 cut(s) 160
PspPI GGNCC 5 cut(s) 160, 161, 1017, 1736, 1767
PspPPI RGGWCCY 1 cut(s) 1767
PstI CTGCAG 2 cut(s) 309, 1975
PsuI RGATCY 2 cut(s) 932, 1522
PvuII CAGCTG 2 cut(s) 1127, 1976
RsaI GTAC 8 cut(s) 181, 696, 854, 1195, 1552, 2033, 2323, 2466
RsaNI GTAC 8 cut(s) 180, 695, 853, 1194, 1551, 2032, 2322, 2465
SacI GAGCTC 1 cut(s) 594
SapI GCTCTTC 2 cut(s) 378, 582
SaqAI TTAA 9 cut(s) 222, 477, 735, 1286, 1452, 1505, 1599, 1724, 2300
Sau3AI GATC 7 cut(s) 283, 496, 810, 895, 932, 1522, 2437
Sau96I GGNCC 5 cut(s) 160, 161, 1017, 1736, 1767
ScaI AGTACT 1 cut(s) 1552
SchI GAGTC 6 cut(s) 518, 644, 653, 1271, 1901, 2272
ScrFI CCNGG 4 cut(s) 761, 1704, 2380, 2724
SduI GDGCHC 4 cut(s) 164, 594, 1224, 1436
SexAI ACCWGGT 1 cut(s) 2722
SfaNI GCATC 9 cut(s) 454, 1004, 1164, 1333, 1456, 1874, 2148, 2305, 2453
SfcI CTRYAG 3 cut(s) 305, 822, 1971
Sfr274I CTCGAG 1 cut(s) 1889
SinI GGWCC 3 cut(s) 1017, 1736, 1767
SlaI CTCGAG 1 cut(s) 1889
SmlI CTYRAG 4 cut(s) 814, 1217, 1889, 1953
SmoI CTYRAG 4 cut(s) 814, 1217, 1889, 1953
SseBI AGGCCT 1 cut(s) 820
SsiI CCGC 3 cut(s) 252, 2102, 2276
SspMI CTAG 3 cut(s) 555, 1247, 1989
SstI GAGCTC 1 cut(s) 594
StuI AGGCCT 1 cut(s) 820
StyD4I CCNGG 4 cut(s) 759, 1702, 2378, 2722
StyI CCWWGG 4 cut(s) 909, 1178, 2483, 2849
TaaI ACNGT 7 cut(s) 25, 141, 205, 852, 1531, 1915, 2681
TaiI ACGT 2 cut(s) 2327, 2463
TaqI TCGA 5 cut(s) 1172, 1237, 1581, 1890, 2619
TatI WGTACW 4 cut(s) 852, 1193, 1550, 2031
TauI GCSGC 2 cut(s) 254, 2279
TfiI GAWTC 9 cut(s) 436, 1005, 1253, 1578, 1583, 1742, 1879, 2201, 2375
Tru1I TTAA 9 cut(s) 222, 477, 735, 1286, 1452, 1505, 1599, 1724, 2300
Tru9I TTAA 9 cut(s) 222, 477, 735, 1286, 1452, 1505, 1599, 1724, 2300
TscAI CASTG 5 cut(s) 1881, 2070, 2136, 2686, 2698
TseFI GTSAC 6 cut(s) 389, 419, 980, 1263, 1291, 1801
TseI GCWGC 9 cut(s) 17, 401, 548, 551, 797, 860, 1970, 1973, 2663
Tsp45I GTSAC 6 cut(s) 389, 419, 980, 1263, 1291, 1801
TspDTI ATGAA 8 cut(s) 365, 1050, 1616, 1835, 2279, 2348, 2529, 2772
TspRI CASTG 5 cut(s) 1881, 2070, 2136, 2686, 2698
Van91I CCANNNNNTGG 1 cut(s) 1763
VpaK11BI GGWCC 3 cut(s) 1017, 1736, 1767
XapI RAATTY 5 cut(s) 74, 1092, 2308, 2368, 2594
XbaI TCTAGA 1 cut(s) 1246
XceI RCATGY 2 cut(s) 908, 1779
XcmI CCANNNNNNNNNTGG 1 cut(s) 974
XhoI CTCGAG 1 cut(s) 1889
XmnI GAANNNNTTC 1 cut(s) 1996
XspI CTAG 3 cut(s) 555, 1247, 1989
ZrmI AGTACT 1 cut(s) 1552
Zsp2I ATGCAT 1 cut(s) 1513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.