Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006376

Physical Location & Seq

Genomic Coordinates Reverse (-)

40789323 .. 40796678

Gene Structure 7356 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1272800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1920 bp

ATGCCTGACCCTCCTATATCTACTCTCCGGCGCAATTCCGCGGTCACGATCACCGGCAACCGGCAAAAGACCGGCGAGGAATTCGTGGGTGGTGTCATATATCTGGCCCAAGGCCTGCTCCAACTCGGCCTATGTTCCGGCGACGTCATTTCCATTGCTGCCTTCAACAGTGAATTGTATTCGGAGTGGTTACTAGCCATTGCAGATGTTGGTGCCATAGCTGCTCCTCTTAATTACCGATGGAGCTATGAAGAAGCACTTTTGGCAATGGAAGTAGTGAGGCCAGCAATGTTGGTGACTGATGATAGCTGCATCCCTTGGTACTCAAAGCTTCAACTCCGCGATGTATGTACCTTCTCTGAGGTGGCATGTTTCGTTGGATTCGCCTTCCTCGGACGTCGAACCTCTGGAAGGCCAAAGGGAGTTAACCTAAGCCACTCGGCTTTTATCATACAGTCCCCATCGAAAATCGCCATTGTTGGCTACGGTGAGGATTATGTTTATTTGCACGCTGCTCCGCTGTGCCACATTGGTGGTTTGTCATCTGCCCTGGCAATGCTGATGGTGGGAGCGTGCCATGTCTTGATGCCCAAGTTTGAGGCTAAATCCGCCCTCGAATCCATAGAGCTACATAGCGTGACTTCTCTGATCACCGTTCCGGCAATGATGGCCAATTTGGTTTCTAGATTCAGTGCACACATGAGTCTTGAACTGTTGTTTTTCGAAGTTCATGTTCCGAAATCTGTCGGGGAGAAGGAAACATGGAAAGGGATAGATAGTGTGAAGAAGGTATTAAATGGAGGTGGAGGCTTGTCAATTGAGCTGACCAAAGCTGCCATTAAATTGTTCCCAAAAGCTAAGCTTCTCTCAGCTTATGGAATGACGGAGACATGCTCGTCGTTAACCTTCATGACTCTGTACGAACCAACTGCTGACACCGCCGGCCAGCCGCTAAGAATGGTTCAACAAGGAAGAGGTGTTTATGTTGGGAAGCCTTCTCCTCACGTAGAACTGAAGATCTCAGTTGACGGCTCTTCTCGTGTGGGGAGGATTTTAACTAGAGGCCAGCATGTCATGCTCGGATATTGGCATGGAAGTCCACCAAATGCGTCAGTTTCCGGTGATAAAGTATGGTTTGACGCCGGTGACATTGGATCCACTGATGACTCAGGTAATGTATGGCTAATCGGACGAGCAAATGGTCGGATCAAAAGTGGAGGGGAGAACATTTACCCTGAAGAGATACGAGAGTTTCAAAGTGGAGCGCACCCTGTTACAACCAGAGGCGGAGCCACAGTGGGGTCAATGGGGTTGCACGACCCCTTGGAAGCCATGGAAATAACCTTAGAGGTCCCTTGGAGCTGCTGGTGCGACCCCTTGGAGCTGCTGGTGCGACCTCTTGGAGCTGCACACCACGTGTTCGATGAAATGTCTAAAAGAAGAAAAAGACTTCGGTTGCAGGTTGAAGAAGAAGGAAGGGCGCGCGCGCGAGAAGAAAAAGACTTCGGTTGCAGGTTCTGTGTAGAGTTTTGTTGTGTGCACCTCGTTACAGTGGCGAAGCCAGGATTTCTCGGGGGGAGGAGCGAACTTAAAAGAATGCAAGGGAAAGTGAAGGCACTTTTTAGTATGGTAACTATCGGCGAGAGGCTGATGACTTGCACTCTGTGGTCGAATACTTCTCTAGGGCGAAACGTGCACCCAGGGCAATTCTTGGACACAGTAAAGGATGCCCGCAATGTCAAAATCAAGAGGACAAAGGATGCTGTCAAGTTCAAGGTTCGGTGCTCCAAGTTTAAGATACCAAAAATATTCATTCTATGGAAGAAGCCATTTCCGGCAACTACGACTGGAAAAATCAGAAGAGACCAAGTCCGAGAAGAAGCTGTATTTCATCTGCAACAATTGAATAGCAATCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006732 GO:0006766 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0016020 GO:0031090 GO:0031903 GO:0031967 GO:0031969 GO:0031975 GO:0042170 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0051186 GO:0051188 GO:0071704 GO:0098588 GO:0098805 GO:1901576 GO:1901661 GO:1901663

KEGG Pathways

Metabolic & Signaling

ko00130 ko01100 ko01110 map00130 map01100 map01110

Pfam Domains

Protein Families

AMP-binding AMP-binding_C

Protein Analysis

640

Amino Acids

70.28

Weight (kDa)

9.05

Isoelectric Point (pI)

41.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AMP-binding	PF00501	18 - 102	4.7e-09	AMP-binding enzyme
AMP-binding	PF00501	135 - 363	8.2e-33	AMP-binding enzyme
Ribosomal_L38e	PF01781	574 - 598	1e-05	Ribosomal L38e protein family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53770
fragaria_vesca	FvH4_3g20190 FvH4_3g20190 FvH4_3g20190 FvH4_3g20190 FvH4_3g20190
malus_domestica	MD01G1239400.v1.1 MD03G1226500.v1.1 MD05G1272800.v1.1 MD08G1067800.v1.1 MD10G1025000.v1.1 MD11G1244500.v1.1 MD11G1244600.v1.1
prunus_persica	Prupe.4G182900_v2.0.a1
pyrus_communis	pycom03g17420
rosa_chinensis	RchiOBHm_Chr5g0034001
rosa_laevigata	RLG00000033518
rosa_multiflora	Rmu_sc0012341.1_g000005 Rmu_sc0014753.1_g000002
rosa_roxburghii	Rroxscaffold_1G00046230
rosa_rugosa	Rorug05G0144100
rosa_samantha	Rh5AG235000 Rh5BG235800 Rh5CG265600 Rh5DG242200
rosa_wichuraiana	Rw5G021220 Rw5G021460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	895
AatII	GACGTC	2 cut(s)	147, 400
Acc36I	ACCTGC	2 cut(s)	1450, 1503
AccB1I	GGYRCC	1 cut(s)	212
AccII	CGCG	6 cut(s)	41, 342, 1483, 1485, 1487, 1489
AciI	CCGC	9 cut(s)	39, 41, 340, 518, 609, 939, 950, 1287, 1732
AclWI	GGATC	3 cut(s)	1149, 1162, 1214
AcoI	YGGCCR	2 cut(s)	669, 943
AcsI	RAATTY	1 cut(s)	80
AcuI	CTGAAG	2 cut(s)	1034, 1257
AcvI	CACGTG	1 cut(s)	1417
AcyI	GRCGYC	3 cut(s)	144, 397, 1140
AdeI	CACNNNGTG	2 cut(s)	1417, 1665
AfaI	GTAC	3 cut(s)	323, 352, 920
AfiI	CCNNNNNNNGG	3 cut(s)	60, 411, 1299
AflIII	ACRYGT	1 cut(s)	1416
AgsI	TTSAA	8 cut(s)	166, 335, 710, 965, 1256, 1466, 1774, 1906
AjnI	CCWGG	3 cut(s)	549, 1561, 1699
AleI	CACNNNNGTG	1 cut(s)	531
Alw21I	GWGCWC	4 cut(s)	697, 1542, 1698, 1787
Alw26I	GTCTC	2 cut(s)	881, 1857
Alw44I	GTGCAC	3 cut(s)	693, 1538, 1694
AlwI	GGATC	3 cut(s)	1149, 1162, 1214
AlwNI	CAGNNNCTG	1 cut(s)	1518
Ama87I	CYCGRG	1 cut(s)	1571
AoxI	GGCC	8 cut(s)	105, 112, 127, 281, 413, 669, 943, 1063
ApaLI	GTGCAC	3 cut(s)	693, 1538, 1694
ApeKI	GCWGC	8 cut(s)	158, 221, 309, 512, 833, 1362, 1384, 1406
ApoI	RAATTY	1 cut(s)	80
AspLEI	GCGC	6 cut(s)	33, 1267, 1483, 1485, 1487, 1489
AspS9I	GGNCC	2 cut(s)	106, 1351
AsuHPI	GGTGA	6 cut(s)	43, 307, 500, 643, 1133, 1157
AsuII	TTCGAA	1 cut(s)	723
AvaI	CYCGRG	1 cut(s)	1571
AvaII	GGWCC	1 cut(s)	1351
BaeGI	GKGCMC	3 cut(s)	697, 1542, 1698
BaeI	ACNNNNGTAYC	2 cut(s)	1235, 1268
BalI	TGGCCA	1 cut(s)	671
BamHI	GGATCC	1 cut(s)	1154
BanI	GGYRCC	1 cut(s)	212
BauI	CACGAG	1 cut(s)	1038
BbrPI	CACGTG	1 cut(s)	1417
Bbv12I	GWGCWC	4 cut(s)	697, 1542, 1698, 1787
BbvI	GCAGC	8 cut(s)	145, 208, 296, 499, 820, 1349, 1371, 1393
BccI	CCATC	4 cut(s)	234, 469, 556, 661
BceAI	ACGGC	1 cut(s)	1045
BcgI	CGANNNNNNTGC	2 cut(s)	911, 945
BciT130I	CCWGG	3 cut(s)	551, 1563, 1701
BclI	TGATCA	1 cut(s)	648
BcoDI	GTCTC	2 cut(s)	881, 1857
BfaI	CTAG	4 cut(s)	194, 684, 1059, 1682
BfuAI	ACCTGC	2 cut(s)	1450, 1503
BglII	AGATCT	1 cut(s)	1017
BisI	GCNGC	9 cut(s)	159, 222, 310, 513, 834, 950, 1363, 1385, 1407
BlpI	GCTNAGC	1 cut(s)	858
BlsI	GCNGC	9 cut(s)	160, 223, 311, 514, 835, 951, 1364, 1386, 1408
Bme1390I	CCNGG	3 cut(s)	551, 1563, 1701
Bme18I	GGWCC	1 cut(s)	1351
BmeT110I	CYCGRG	1 cut(s)	1571
BmgT120I	GGNCC	2 cut(s)	106, 1351
BmiI	GGNNCC	4 cut(s)	214, 1156, 1291, 1353
BmrFI	CCNGG	3 cut(s)	551, 1563, 1701
BmsI	GCATC	4 cut(s)	321, 576, 1717, 1750
BplI	GAGNNNNNCTC	2 cut(s)	878, 910
Bpu10I	CCTNAGC	1 cut(s)	431
Bpu1102I	GCTNAGC	1 cut(s)	858
Bpu14I	TTCGAA	1 cut(s)	723
BsaAI	YACGTR	2 cut(s)	1006, 1417
BsaHI	GRCGYC	3 cut(s)	144, 397, 1140
BsaI	GGTCTC	1 cut(s)	1857
BsaWI	WCCGGW	1 cut(s)	1118
BsaXI	ACNNNNNCTCC	2 cut(s)	1281, 1311
Bsc4I	CCNNNNNNNGG	3 cut(s)	60, 411, 1299
Bse118I	RCCGGY	5 cut(s)	53, 60, 71, 941, 1142
Bse1I	ACTGG	1 cut(s)	1852
Bse3DI	GCAATG	7 cut(s)	153, 198, 273, 294, 561, 669, 1741
BseBI	CCWGG	3 cut(s)	551, 1563, 1701
BseGI	GGATG	3 cut(s)	312, 1732, 1765
BseLI	CCNNNNNNNGG	3 cut(s)	60, 411, 1299
BseMI	GCAATG	7 cut(s)	153, 198, 273, 294, 561, 669, 1741
BseMII	CTCAG	4 cut(s)	351, 882, 1035, 1182
BseNI	ACTGG	1 cut(s)	1852
BsePI	GCGCGC	3 cut(s)	1481, 1483, 1485
BseRI	GAGGAG	3 cut(s)	216, 990, 1594
BseSI	GKGCMC	3 cut(s)	697, 1542, 1698
BseXI	GCAGC	8 cut(s)	145, 208, 296, 499, 820, 1349, 1371, 1393
BsgI	GTGCAG	1 cut(s)	1392
Bsh1236I	CGCG	6 cut(s)	41, 342, 1483, 1485, 1487, 1489
BshFI	GGCC	8 cut(s)	107, 114, 129, 283, 415, 671, 945, 1065
BshNI	GGYRCC	1 cut(s)	212
BsiHKAI	GWGCWC	4 cut(s)	697, 1542, 1698, 1787
BsiHKCI	CYCGRG	1 cut(s)	1571
BslFI	GGGAC	2 cut(s)	442, 1337
BslI	CCNNNNNNNGG	3 cut(s)	60, 411, 1299
BsmAI	GTCTC	2 cut(s)	881, 1857
BsmFI	GGGAC	2 cut(s)	442, 1337
BsmI	GAATGC	1 cut(s)	1602
BsnI	GGCC	8 cut(s)	107, 114, 129, 283, 415, 671, 945, 1065
Bso31I	GGTCTC	1 cut(s)	1857
BsoBI	CYCGRG	1 cut(s)	1571
Bsp119I	TTCGAA	1 cut(s)	723
Bsp1286I	GDGCHC	4 cut(s)	697, 1542, 1698, 1787
Bsp143I	GATC	5 cut(s)	48, 648, 1017, 1154, 1206
Bsp1720I	GCTNAGC	1 cut(s)	858
Bsp19I	CCATGG	1 cut(s)	1332
BspACI	CCGC	9 cut(s)	39, 41, 340, 518, 609, 939, 950, 1287, 1732
BspANI	GGCC	8 cut(s)	107, 114, 129, 283, 415, 671, 945, 1065
BspCNI	CTCAG	4 cut(s)	352, 881, 1034, 1181
BspFNI	CGCG	6 cut(s)	41, 342, 1483, 1485, 1487, 1489
BspHI	TCATGA	1 cut(s)	909
BspLI	GGNNCC	4 cut(s)	214, 1156, 1291, 1353
BspMI	ACCTGC	2 cut(s)	1450, 1503
BspPI	GGATC	3 cut(s)	1149, 1162, 1214
BspQI	GCTCTTC	1 cut(s)	1039
BspT104I	TTCGAA	1 cut(s)	723
BspT107I	GGYRCC	1 cut(s)	212
BspTNI	GGTCTC	1 cut(s)	1857
BsrDI	GCAATG	7 cut(s)	153, 198, 273, 294, 561, 669, 1741
BsrFI	RCCGGY	5 cut(s)	53, 60, 71, 941, 1142
BsrI	ACTGG	1 cut(s)	1852
BssAI	RCCGGY	5 cut(s)	53, 60, 71, 941, 1142
BssHII	GCGCGC	3 cut(s)	1481, 1483, 1485
BssMI	GATC	5 cut(s)	48, 648, 1017, 1154, 1206
BssNI	GRCGYC	3 cut(s)	144, 397, 1140
BssSI	CACGAG	1 cut(s)	1038
BssT1I	CCWWGG	6 cut(s)	109, 317, 1323, 1332, 1355, 1377
Bst2BI	CACGAG	1 cut(s)	1038
Bst2UI	CCWGG	3 cut(s)	551, 1563, 1701
Bst4CI	ACNGT	8 cut(s)	170, 456, 488, 655, 714, 1297, 1552, 1720
Bst6I	CTCTTC	4 cut(s)	967, 1039, 1233, 1855
BstACI	GRCGYC	3 cut(s)	144, 397, 1140
BstAPI	GCANNNNNTGC	1 cut(s)	1075
BstBAI	YACGTR	2 cut(s)	1006, 1417
BstBI	TTCGAA	1 cut(s)	723
BstDEI	CTNAG	8 cut(s)	360, 431, 858, 868, 953, 1021, 1168, 1345
BstDSI	CCRYGG	2 cut(s)	39, 1332
BstENI	CCTNNNNNAGG	1 cut(s)	409
BstF5I	GGATG	3 cut(s)	312, 1732, 1765
BstFNI	CGCG	6 cut(s)	41, 342, 1483, 1485, 1487, 1489
BstHHI	GCGC	6 cut(s)	33, 1267, 1483, 1485, 1487, 1489
BstKTI	GATC	5 cut(s)	51, 651, 1020, 1157, 1209
BstMAI	GTCTC	2 cut(s)	881, 1857
BstMBI	GATC	5 cut(s)	48, 648, 1017, 1154, 1206
BstNI	CCWGG	3 cut(s)	551, 1563, 1701
BstNSI	RCATGY	3 cut(s)	372, 894, 1073
BstSCI	CCNGG	3 cut(s)	549, 1561, 1699
BstSLI	GKGCMC	3 cut(s)	697, 1542, 1698
BstUI	CGCG	6 cut(s)	41, 342, 1483, 1485, 1487, 1489
BstV1I	GCAGC	8 cut(s)	145, 208, 296, 499, 820, 1349, 1371, 1393
BstX2I	RGATCY	2 cut(s)	1017, 1154
BstXI	CCANNNNNNTGG	1 cut(s)	533
BstYI	RGATCY	2 cut(s)	1017, 1154
BsuRI	GGCC	8 cut(s)	107, 114, 129, 283, 415, 671, 945, 1065
BtgI	CCRYGG	2 cut(s)	39, 1332
BtgZI	GCGATG	1 cut(s)	357
BtsCI	GGATG	3 cut(s)	312, 1732, 1765
BtsIMutI	CAGTG	5 cut(s)	175, 697, 1158, 1302, 1557
BveI	ACCTGC	2 cut(s)	1450, 1503
CaiI	CAGNNNCTG	1 cut(s)	1518
CciI	TCATGA	1 cut(s)	909
CfoI	GCGC	6 cut(s)	33, 1267, 1483, 1485, 1487, 1489
Cfr10I	RCCGGY	5 cut(s)	53, 60, 71, 941, 1142
Cfr13I	GGNCC	2 cut(s)	106, 1351
Cfr42I	CCGCGG	1 cut(s)	42
CseI	GACGC	2 cut(s)	1098, 1148
Csp6I	GTAC	3 cut(s)	322, 351, 919
CviQI	GTAC	3 cut(s)	322, 351, 919
DdeI	CTNAG	8 cut(s)	360, 431, 858, 868, 953, 1021, 1168, 1345
DpnI	GATC	5 cut(s)	50, 650, 1019, 1156, 1208
DpnII	GATC	5 cut(s)	48, 648, 1017, 1154, 1206
DraIII	CACNNNGTG	2 cut(s)	1417, 1665
DrdI	GACNNNNNNGTC	1 cut(s)	895
DseDI	GACNNNNNNGTC	1 cut(s)	895
EaeI	YGGCCR	2 cut(s)	669, 943
Eam1104I	CTCTTC	4 cut(s)	967, 1039, 1233, 1855
EarI	CTCTTC	4 cut(s)	967, 1039, 1233, 1855
EciI	GGCGGA	2 cut(s)	598, 1302
Eco130I	CCWWGG	6 cut(s)	109, 317, 1323, 1332, 1355, 1377
Eco147I	AGGCCT	1 cut(s)	114
Eco31I	GGTCTC	1 cut(s)	1857
Eco47I	GGWCC	1 cut(s)	1351
Eco57I	CTGAAG	2 cut(s)	1034, 1257
Eco72I	CACGTG	1 cut(s)	1417
Eco88I	CYCGRG	1 cut(s)	1571
EcoNI	CCTNNNNNAGG	1 cut(s)	409
EcoO109I	RGGNCCY	1 cut(s)	1351
EcoRI	GAATTC	1 cut(s)	80
EcoRII	CCWGG	3 cut(s)	549, 1561, 1699
EcoT14I	CCWWGG	6 cut(s)	109, 317, 1323, 1332, 1355, 1377
ErhI	CCWWGG	6 cut(s)	109, 317, 1323, 1332, 1355, 1377
FalI	AAGNNNNNCTT	4 cut(s)	243, 275, 846, 878
FaqI	GGGAC	2 cut(s)	442, 1337
FauI	CCCGC	1 cut(s)	1739
FbaI	TGATCA	1 cut(s)	648
Fnu4HI	GCNGC	9 cut(s)	159, 222, 310, 513, 834, 950, 1363, 1385, 1407
FokI	GGATG	3 cut(s)	299, 1739, 1772
Fsp4HI	GCNGC	9 cut(s)	159, 222, 310, 513, 834, 950, 1363, 1385, 1407
FspBI	CTAG	4 cut(s)	194, 684, 1059, 1682
GlaI	GCGC	6 cut(s)	32, 1266, 1482, 1484, 1486, 1488
GluI	GCNGC	9 cut(s)	159, 222, 310, 513, 834, 950, 1363, 1385, 1407
HaeIII	GGCC	8 cut(s)	107, 114, 129, 283, 415, 671, 945, 1065
HgaI	GACGC	2 cut(s)	1098, 1148
HhaI	GCGC	6 cut(s)	33, 1267, 1483, 1485, 1487, 1489
Hin1I	GRCGYC	3 cut(s)	144, 397, 1140
Hin6I	GCGC	6 cut(s)	31, 1265, 1481, 1483, 1485, 1487
HinP1I	GCGC	6 cut(s)	31, 1265, 1481, 1483, 1485, 1487
HincII	GTYRAC	3 cut(s)	427, 903, 1027
HindII	GTYRAC	3 cut(s)	427, 903, 1027
HindIII	AAGCTT	2 cut(s)	329, 860
HinfI	GANTC	6 cut(s)	381, 617, 687, 703, 913, 1166
HpaI	GTTAAC	2 cut(s)	427, 903
HphI	GGTGA	6 cut(s)	43, 307, 500, 643, 1133, 1157
Hpy166II	GTNNAC	7 cut(s)	427, 695, 903, 1027, 1100, 1540, 1696
Hpy188III	TCNNGA	7 cut(s)	46, 408, 583, 684, 707, 910, 1747
Hpy8I	GTNNAC	7 cut(s)	427, 695, 903, 1027, 1100, 1540, 1696
Hpy99I	CGWCG	3 cut(s)	146, 402, 901
HpyCH4III	ACNGT	8 cut(s)	170, 456, 488, 655, 714, 1297, 1552, 1720
HpyCH4IV	ACGT	5 cut(s)	144, 397, 1005, 1416, 1692
HpyF3I	CTNAG	8 cut(s)	360, 431, 858, 868, 953, 1021, 1168, 1345
HpySE526I	ACGT	5 cut(s)	144, 397, 1005, 1416, 1692
Hsp92I	GRCGYC	3 cut(s)	144, 397, 1140
HspAI	GCGC	6 cut(s)	31, 1265, 1481, 1483, 1485, 1487
KroI	GCCGGC	1 cut(s)	941
KroNI	GCCGGC	1 cut(s)	943
Ksp22I	TGATCA	1 cut(s)	648
KspAI	GTTAAC	2 cut(s)	427, 903
KspI	CCGCGG	1 cut(s)	42
Kzo9I	GATC	5 cut(s)	48, 648, 1017, 1154, 1206
LguI	GCTCTTC	1 cut(s)	1039
Lsp1109I	GCAGC	8 cut(s)	145, 208, 296, 499, 820, 1349, 1371, 1393
LweI	GCATC	4 cut(s)	321, 576, 1717, 1750
MaeI	CTAG	4 cut(s)	194, 684, 1059, 1682
MaeII	ACGT	5 cut(s)	144, 397, 1005, 1416, 1692
MaeIII	GTNAC	8 cut(s)	43, 189, 295, 637, 1145, 1273, 1546, 1630
MalI	GATC	5 cut(s)	50, 650, 1019, 1156, 1208
MauBI	CGCGCGCG	2 cut(s)	1483, 1485
MboI	GATC	5 cut(s)	48, 648, 1017, 1154, 1206
MfeI	CAATTG	2 cut(s)	816, 1901
MflI	RGATCY	2 cut(s)	1017, 1154
MhlI	GDGCHC	4 cut(s)	697, 1542, 1698, 1787
MlsI	TGGCCA	1 cut(s)	671
MluCI	AATT	9 cut(s)	34, 80, 173, 232, 673, 816, 842, 1706, 1901
MluNI	TGGCCA	1 cut(s)	671
MlyI	GAGTC	3 cut(s)	712, 907, 1160
MmeI	TCCRAC	3 cut(s)	145, 358, 1184
Mox20I	TGGCCA	1 cut(s)	671
MroNI	GCCGGC	1 cut(s)	941
MscI	TGGCCA	1 cut(s)	671
MseI	TTAA	8 cut(s)	231, 426, 794, 840, 902, 1055, 1590, 1794
MslI	CAYNNNNRTG	1 cut(s)	531
Msp20I	TGGCCA	1 cut(s)	671
MspA1I	CMGCKG	2 cut(s)	41, 520
MspR9I	CCNGG	3 cut(s)	551, 1563, 1701
MunI	CAATTG	2 cut(s)	816, 1901
Mva1269I	GAATGC	1 cut(s)	1602
MvaI	CCWGG	3 cut(s)	551, 1563, 1701
MvnI	CGCG	6 cut(s)	41, 342, 1483, 1485, 1487, 1489
NaeI	GCCGGC	1 cut(s)	943
NcoI	CCATGG	1 cut(s)	1332
NdeII	GATC	5 cut(s)	48, 648, 1017, 1154, 1206
NgoMIV	GCCGGC	1 cut(s)	941
NlaIV	GGNNCC	4 cut(s)	214, 1156, 1291, 1353
NmeAIII	GCCGAG	2 cut(s)	105, 419
NmuCI	GTSAC	4 cut(s)	43, 295, 637, 1145
NspI	RCATGY	3 cut(s)	372, 894, 1073
NspV	TTCGAA	1 cut(s)	723
OliI	CACNNNNGTG	1 cut(s)	531
PagI	TCATGA	1 cut(s)	909
PasI	CCCWGGG	1 cut(s)	1700
PauI	GCGCGC	3 cut(s)	1481, 1483, 1485
PceI	AGGCCT	1 cut(s)	114
PciSI	GCTCTTC	1 cut(s)	1039
PctI	GAATGC	1 cut(s)	1602
PdiI	GCCGGC	1 cut(s)	943
PfeI	GAWTC	3 cut(s)	381, 617, 687
PflFI	GACNNNGTC	1 cut(s)	1868
PkrI	GCNGC	9 cut(s)	160, 223, 311, 514, 835, 951, 1364, 1386, 1408
PleI	GAGTC	3 cut(s)	711, 907, 1160
PmaCI	CACGTG	1 cut(s)	1417
PmlI	CACGTG	1 cut(s)	1417
PpsI	GAGTC	3 cut(s)	711, 907, 1160
Ppu21I	YACGTR	2 cut(s)	1006, 1417
PpuMI	RGGWCCY	1 cut(s)	1351
Psp5II	RGGWCCY	1 cut(s)	1351
Psp6I	CCWGG	3 cut(s)	549, 1561, 1699
PspCI	CACGTG	1 cut(s)	1417
PspGI	CCWGG	3 cut(s)	549, 1561, 1699
PspN4I	GGNNCC	4 cut(s)	214, 1156, 1291, 1353
PspPI	GGNCC	2 cut(s)	106, 1351
PspPPI	RGGWCCY	1 cut(s)	1351
PstNI	CAGNNNCTG	1 cut(s)	1518
PsuI	RGATCY	2 cut(s)	1017, 1154
PsyI	GACNNNGTC	1 cut(s)	1868
PteI	GCGCGC	3 cut(s)	1481, 1483, 1485
RsaI	GTAC	3 cut(s)	323, 352, 920
RsaNI	GTAC	3 cut(s)	322, 351, 919
RseI	CAYNNNNRTG	1 cut(s)	531
SacII	CCGCGG	1 cut(s)	42
SapI	GCTCTTC	1 cut(s)	1039
SaqAI	TTAA	8 cut(s)	231, 426, 794, 840, 902, 1055, 1590, 1794
SatI	GCNGC	9 cut(s)	159, 222, 310, 513, 834, 950, 1363, 1385, 1407
Sau3AI	GATC	5 cut(s)	48, 648, 1017, 1154, 1206
Sau96I	GGNCC	2 cut(s)	106, 1351
SchI	GAGTC	3 cut(s)	712, 907, 1160
ScrFI	CCNGG	3 cut(s)	551, 1563, 1701
SduI	GDGCHC	4 cut(s)	697, 1542, 1698, 1787
SfaNI	GCATC	4 cut(s)	321, 576, 1717, 1750
Sfr303I	CCGCGG	1 cut(s)	42
SfuI	TTCGAA	1 cut(s)	723
SgrAI	CRCCGGYG	1 cut(s)	1142
SgrBI	CCGCGG	1 cut(s)	42
SinI	GGWCC	1 cut(s)	1351
SmiMI	CAYNNNNRTG	1 cut(s)	531
Sse9I	AATT	9 cut(s)	34, 80, 173, 232, 673, 816, 842, 1706, 1901
SseBI	AGGCCT	1 cut(s)	114
SsiI	CCGC	9 cut(s)	39, 41, 340, 518, 609, 939, 950, 1287, 1732
SspI	AATATT	1 cut(s)	1809
SspMI	CTAG	4 cut(s)	194, 684, 1059, 1682
StuI	AGGCCT	1 cut(s)	114
StyD4I	CCNGG	3 cut(s)	549, 1561, 1699
StyI	CCWWGG	6 cut(s)	109, 317, 1323, 1332, 1355, 1377
TaaI	ACNGT	8 cut(s)	170, 456, 488, 655, 714, 1297, 1552, 1720
TaiI	ACGT	5 cut(s)	147, 400, 1008, 1419, 1695
TaqI	TCGA	6 cut(s)	400, 464, 615, 723, 1422, 1670
TasI	AATT	9 cut(s)	34, 80, 173, 232, 673, 816, 842, 1706, 1901
TauI	GCSGC	1 cut(s)	952
TfiI	GAWTC	3 cut(s)	381, 617, 687
Tru1I	TTAA	8 cut(s)	231, 426, 794, 840, 902, 1055, 1590, 1794
Tru9I	TTAA	8 cut(s)	231, 426, 794, 840, 902, 1055, 1590, 1794
TscAI	CASTG	5 cut(s)	175, 697, 1165, 1302, 1557
TseFI	GTSAC	4 cut(s)	43, 295, 637, 1145
TseI	GCWGC	8 cut(s)	158, 221, 309, 512, 833, 1362, 1384, 1406
Tsp45I	GTSAC	4 cut(s)	43, 295, 637, 1145
TspDTI	ATGAA	6 cut(s)	264, 719, 898, 1440, 1801, 1880
TspGWI	ACGGA	1 cut(s)	899
TspRI	CASTG	5 cut(s)	175, 697, 1165, 1302, 1557
Tth111I	GACNNNGTC	1 cut(s)	1868
VneI	GTGCAC	3 cut(s)	693, 1538, 1694
VpaK11BI	GGWCC	1 cut(s)	1351
XagI	CCTNNNNNAGG	1 cut(s)	409
XapI	RAATTY	1 cut(s)	80
XbaI	TCTAGA	1 cut(s)	683
XceI	RCATGY	3 cut(s)	372, 894, 1073
XspI	CTAG	4 cut(s)	194, 684, 1059, 1682
ZraI	GACGTC	2 cut(s)	145, 398

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.