Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004470

Physical Location & Seq

Genomic Coordinates Forward (+)

43401676 .. 43407802

Gene Structure 6127 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1300900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3228 bp

ATGCCGCTGGTGAGGTTTCAGGTGCGGAACGAGTACAGCTTGGGGCAGCCTCAGTTATACAAGGAGGTAAACAGAGAAGATCCCAAAGCTGTGCTCGACGGCGTCGCCGTCTCTGGCCTCGTTGGGATCTTACGCCAATTGGGGGATCTTGCCGAGTTTGCAGCAGAGGTCTTTCATGGCTTGCAAGAGCAAGTGATGACTACAGCTTCTAGAAGCCATAAACTGATTGTTCGTGTGCAACACATTGAAGCTGCACTTCCTTCCCTCGAGAAGGCTGTACTTGCTCAAACAAGCCACATACATTTTGCTTACACCGGTGGTATAGAGTGGCATCCTCGTATTCGAAATGAGAAAAATCATTTGATCTACAATGACTTGCCGCGTTTTATTATGGATTCATACGAAGAATGTCGGGATCCTCCACGTTTGGATTTACTGGACAAATATGATACTGGTGGCCCAGGATCTTGTTTGAAGAGATATTCAGATCCAACCTTTTTCAAAAAAGCATCAGCTATCCCTGATGAAGAAAATGTTGAAAAAGTCCGAAGATCTAGGAAAGCTCAAAGAAGCAAGAAGAAAAGAGGATCACAACGTAATGGAGATGTATTGCGTGGTGCATCAGCATCTAATCGCAGTAGCAGAATGCAACATATTTCTCCCATTGTAAATGGGCGAAGCTCATCTTCTCAAACTGCCTCTACAGCAGACATGGCATTAAAATCTGACCTGGGAGACAATTCCATTTCTTTTGATTCAAGAACTGAGTTGGGATATATTGAGTATGCTGCCCATCCAACTTCCTCCATGCAAGCTGAAGAACATGAATCCAAGGAATCACCTACTTCTAAGTTGGTGCAACATGATGATACTATTGATTCTGTTTCGCCTGCTGATCAAACTGGATTTGTAGGTGATAACTTTCCTGGAAGTTCATTACAGGATCAAGTTACCTCTGGTTCATCTTGTGTTAACTGGGATGAGAAAACAGAGATAGTGGATCCAAAATGTCAACAAAATGGTATAGATGAAAATACAGAGATACATCCAACAAAAGATGACTTGGATGCACATGAAGCCGGAGCTGGTAACTTTAGAATTGTTGAGCGAATGGATGCCCTCTTTGTTGATGAAAACATTGTGGAATCAATTTTGATCAGGAATCAGACTGATGAAATTGAAAGTGAACCAGACAATTTCATGGATGCTCTTAACACCATTGAATCAGAATCTGAAACCGATTTAGATTGTCAAACAAAACGAGAAGTTGAACATTTCGCCTCTGTTGTCAGCAACAAAGAACCATACGGGGTGCATGACATTAGCCTCGATTGTTCAGGCCTTCAAACTACAACTTTTGAATTTCATACTTCAACATCTTACATTTCCTTCGAAGCAGAAGTGCCGACAGATCTGCCAAACTCAGCCTTGTCAGAGAGTCCTACACATAAGCAGATGCCTCAGATAGACATGGAACCTTCTAATCCAAACCACACTGTAGGCACCAATAGTGCTGACATTTTTTATGGTTCATGGTTAGAATCTGTTAGTGGTGATTCAACATCCTCTGGCTCCGGAACTACTAACTTGGAGGATAAGACCATAAGCAGTTTATATATTCCTCAAGAATCTCCTGCTGACATTTCCAGGAGCAATTCAACATCTCCTTCCTCTGGAGCTACTAACGAGCAGGACAAGACCATAAGCAGTTTATGTGAGGCTCAAGAATCCCCTGCTGACATTTCCAGGAATAATTCAACACCCTCTGGCTCTGGAACTACGAATGTGAAGGATGAGATCACAAGCCGTTTATGTGAGTCTCAAGAATCCCCTGCTGACATTTCCAGGAATAATTCAACACCCTCTGGCTCTGGAACTACGAATGTGAAGGATGAGATCACAAGCCGTTTATGTGAGAATAATTCAACACCCTCTGGCTCTGGAACTATGAATGTGAAGGATGAGATCACAAGCCGTTTATGTGAGTCTCAAGAATCCCCTGCTGAAATTTCCAGGAATAATTCAACACCCTCTGGCTCTGGAACTAAGAATGTGAAGGATGAGATCACAAGCCGTTTATGTGAGTCTCAAGAATCCCCTGCTGACATTTCCAGGAATAATTCCATAAATTTCTGGACTAATGGTGGCCTATTAGGACTTGAGCCATCCAAACCACCTGATTTCACCATGCCAAGTTCTATAAGTCAAGATTCTGCTAACAGAAGTCACAGTGAGACACACGAATGCGAACATAGTCCATTAGTTGAGAATGCTGGCTGTAATGATAAGGATTTGAGTTCTAAATGCTCCATATCATGCTCGGAGAATCAAGATGATGGCATCACTTTCAAGAAAATACCTAACGGATTTTCACCCACTGAGTTGTATCCAAAGCTTCGTAATCTTGGTGATACAATGGAACCTGGGACTGTGTTGCCAGTTGTTCCATATGGAAAATCCACTTCCAATGAAGCGATTCAGGGGAATGAGGAAAGCTCTTCTCGGGTTTTTGGGCTTGGTCGTATGTTACTTGTGAATGGTTTTGGTAGAAAAGCTGCACAGGCTCACGATGACGGATCTGAGCCAGCTAGTTATTCAAATGCTGGTGTACTGGACCAGAAAAATGAGCAGCACAGAGTTGAGTACCAAGCATTTCCGGAGACATCCTTCAGAGATCAGTTTGAGCATGGAACTGGTATTGATTCATCTCCTTCGTCACCACCACTTGAACATATGAAAATATCTTTCCTTCCCGTAGATGGCATTGAAACTTCCATACTCAAACTGAAATTATCTGATGGCAATCAAGGCCATGAAAGCGTAAGAGACATGTTTCAGTCATTTCAGTTGATCCCAGAACCTGCTATTGCCTTGCATGAATTTGGTTCTGATTCCGATGATGACACATTCTGTAGATCATCTCCATATATATCAGATGATTGTCATAGTCAACTCTCGGAGTCGAATTCTGAACAATGGGAATCTAGTGAAACTCTAGAAAGAAATAACCATGGACTGTATGATGGTTTATGTGGAATCTCATCAGAGGAACTTATATCCACCTCCCCGGAACTGGGCGGAATATCCCATAATGCCACTTATGATGATGGTGGAAGTAAAAGTGTGCACACCGAGAATGGTCTGGAACATTCTCTCTCTGATCCTGTACTTGATCTTCCAAGTTTCGATGCTTTGGGACCTGTACTTCAGCAAGAAACAAAAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0003785 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0016020 GO:0016043 GO:0030832 GO:0030833 GO:0030838 GO:0031209 GO:0031334 GO:0032271 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0032991 GO:0033043 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0044087 GO:0044089 GO:0044424 GO:0044444 GO:0044464 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051125 GO:0051127 GO:0051128 GO:0051130 GO:0051493 GO:0051495 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko00620 ko00680 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00680 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1076

Amino Acids

117.4

Weight (kDa)

4.68

Isoelectric Point (pI)

55.12

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004470)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29170 AT1G29170 AT1G29170 AT2G34150 AT2G34150 AT2G34150 AT2G34150 AT2G34150
fragaria_vesca	FvH4_3g07780 FvH4_3g07780 FvH4_3g07780 FvH4_3g07780
malus_domestica	MD00G1207000.v1.1 MD05G1300900.v1.1 MD05G1301000.v1.1 MD10G1279900.v1.1
prunus_persica	Prupe.4G061600_v2.0.a1 Prupe.4G061600_v2.0.a1
pyrus_communis	pycom05g27880 pycom10g23370
rosa_chinensis	RchiOBHm_Chr5g0010801
rosa_laevigata	RLG00000031781
rosa_multiflora	Rmu_sc0000109.1_g000018
rosa_roxburghii	Rroxscaffold_1G00065580
rosa_rugosa	Rorug04G0452600 Rorug04G0452700
rosa_samantha	Rh5AG084800 Rh5BG079600 Rh5CG092300 Rh5DG079700
rosa_wichuraiana	Rw0G000540 Rw0G022250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2869
AccB1I	GGYRCC	1 cut(s)	1502
AccII	CGCG	1 cut(s)	382
AccIII	TCCGGA	2 cut(s)	1574, 2656
AciI	CCGC	4 cut(s)	5, 25, 380, 3078
AcsI	RAATTY	5 cut(s)	1361, 2007, 2128, 2879, 2965
AcuI	CTGAAG	3 cut(s)	837, 2653, 3191
AcyI	GRCGYC	1 cut(s)	102
AfaI	GTAC	6 cut(s)	35, 279, 2610, 2645, 3168, 3204
AfiI	CCNNNNNNNGG	6 cut(s)	142, 271, 1673, 2759, 3073, 3074
AflIII	ACRYGT	1 cut(s)	2829
AgeI	ACCGGT	1 cut(s)	314
AjnI	CCWGG	9 cut(s)	460, 729, 925, 1646, 1745, 1844, 2012, 2111, 2422
Alw21I	GWGCWC	2 cut(s)	96, 3129
Alw26I	GTCTC	8 cut(s)	115, 729, 1824, 1992, 2091, 2228, 2654, 2820
Alw44I	GTGCAC	1 cut(s)	3125
AlwNI	CAGNNNCTG	2 cut(s)	1232, 2861
Ama87I	CYCGRG	2 cut(s)	266, 2502
Aor13HI	TCCGGA	2 cut(s)	1574, 2656
AoxI	GGCC	5 cut(s)	115, 457, 1339, 2146, 2809
ApaLI	GTGCAC	1 cut(s)	3125
ApeKI	GCWGC	6 cut(s)	46, 161, 251, 788, 2555, 2629
ApoI	RAATTY	5 cut(s)	1361, 2007, 2128, 2879, 2965
AsiGI	ACCGGT	1 cut(s)	314
Asp700I	GAANNNNTTC	1 cut(s)	2475
AspS9I	GGNCC	3 cut(s)	458, 2614, 3197
AsuC2I	CCSGG	1 cut(s)	3068
AsuHPI	GGTGA	8 cut(s)	22, 831, 926, 1565, 2176, 2364, 2420, 2709
AsuII	TTCGAA	2 cut(s)	343, 1392
AvaI	CYCGRG	2 cut(s)	266, 2502
AvaII	GGWCC	2 cut(s)	2614, 3197
BaeGI	GKGCMC	1 cut(s)	3129
BamHI	GGATCC	2 cut(s)	415, 1000
BanI	GGYRCC	1 cut(s)	1502
BarI	GAAGNNNNNNTAC	2 cut(s)	3159, 3191
Bbv12I	GWGCWC	2 cut(s)	96, 3129
BbvI	GCAGC	6 cut(s)	58, 173, 238, 775, 2542, 2641
BccI	CCATC	7 cut(s)	801, 2173, 2330, 2753, 2792, 3017, 3101
BceAI	ACGGC	6 cut(s)	92, 115, 1791, 1890, 1959, 2058
BcgI	CGANNNNNNTGC	2 cut(s)	1396, 1430
BciT130I	CCWGG	9 cut(s)	462, 731, 927, 1648, 1747, 1846, 2014, 2113, 2424
BciVI	GTATCC	1 cut(s)	2397
BclI	TGATCA	2 cut(s)	895, 1155
BcnI	CCSGG	1 cut(s)	3068
BcoDI	GTCTC	8 cut(s)	115, 729, 1824, 1992, 2091, 2228, 2654, 2820
BfaI	CTAG	5 cut(s)	210, 555, 2589, 2985, 2996
BfmI	CTRYAG	4 cut(s)	201, 702, 1497, 2911
BfuAI	ACCTGC	1 cut(s)	2869
BfuI	GTATCC	1 cut(s)	2397
BglII	AGATCT	2 cut(s)	551, 1411
BisI	GCNGC	8 cut(s)	5, 47, 162, 252, 380, 789, 2556, 2630
BlsI	GCNGC	8 cut(s)	6, 48, 163, 253, 381, 790, 2557, 2631
Bme18I	GGWCC	2 cut(s)	2614, 3197
BmeT110I	CYCGRG	2 cut(s)	266, 2502
BmgT120I	GGNCC	3 cut(s)	458, 2614, 3197
BmiI	GGNNCC	7 cut(s)	417, 1002, 1476, 1504, 1573, 2421, 3198
BmrI	ACTGGG	2 cut(s)	985, 3083
BmuI	ACTGGG	2 cut(s)	985, 3083
BplI	GAGNNNNNCTC	2 cut(s)	2480, 2512
BpmI	CTGGAG	1 cut(s)	1695
Bpu14I	TTCGAA	2 cut(s)	343, 1392
BpuEI	CTTGAG	6 cut(s)	1608, 1707, 1806, 1974, 2073, 2180
BpuMI	CCSGG	1 cut(s)	3068
BsaBI	GATNNNNATC	1 cut(s)	2802
BsaHI	GRCGYC	1 cut(s)	102
BsaJI	CCNNGG	6 cut(s)	460, 730, 831, 2423, 3010, 3066
BsaWI	WCCGGW	3 cut(s)	314, 1574, 2656
Bsc4I	CCNNNNNNNGG	6 cut(s)	142, 271, 1673, 2759, 3073, 3074
Bse118I	RCCGGY	1 cut(s)	314
Bse1I	ACTGG	8 cut(s)	441, 457, 907, 980, 2438, 2616, 2698, 3078
Bse8I	GATNNNNATC	1 cut(s)	2802
BseAI	TCCGGA	2 cut(s)	1574, 2656
BseBI	CCWGG	9 cut(s)	462, 731, 927, 1648, 1747, 1846, 2014, 2113, 2424
BseDI	CCNNGG	6 cut(s)	460, 730, 831, 2423, 3010, 3066
BseJI	GATNNNNATC	1 cut(s)	2802
BseLI	CCNNNNNNNGG	6 cut(s)	142, 271, 1673, 2759, 3073, 3074
BseMII	CTCAG	6 cut(s)	65, 756, 1437, 1475, 2370, 2571
BseNI	ACTGG	8 cut(s)	441, 457, 907, 980, 2438, 2616, 2698, 3078
BseSI	GKGCMC	1 cut(s)	3129
BseXI	GCAGC	6 cut(s)	58, 173, 238, 775, 2542, 2641
BsgI	GTGCAG	2 cut(s)	237, 2541
Bsh1236I	CGCG	1 cut(s)	382
BshFI	GGCC	5 cut(s)	117, 459, 1341, 2148, 2811
BshNI	GGYRCC	1 cut(s)	1502
BshTI	ACCGGT	1 cut(s)	314
BsiHKAI	GWGCWC	2 cut(s)	96, 3129
BsiHKCI	CYCGRG	2 cut(s)	266, 2502
BsiSI	CCGG	5 cut(s)	315, 1080, 1575, 2657, 3068
BslFI	GGGAC	2 cut(s)	2440, 3210
BslI	CCNNNNNNNGG	6 cut(s)	142, 271, 1673, 2759, 3073, 3074
BsmAI	GTCTC	8 cut(s)	115, 729, 1824, 1992, 2091, 2228, 2654, 2820
BsmBI	CGTCTC	1 cut(s)	115
BsmFI	GGGAC	2 cut(s)	2440, 3210
BsmI	GAATGC	3 cut(s)	651, 2249, 2275
BsnI	GGCC	5 cut(s)	117, 459, 1341, 2148, 2811
BsoBI	CYCGRG	2 cut(s)	266, 2502
Bsp119I	TTCGAA	2 cut(s)	343, 1392
Bsp1286I	GDGCHC	2 cut(s)	96, 3129
Bsp13I	TCCGGA	2 cut(s)	1574, 2656
Bsp19I	CCATGG	1 cut(s)	3010
BspACI	CCGC	4 cut(s)	5, 25, 380, 3078
BspANI	GGCC	5 cut(s)	117, 459, 1341, 2148, 2811
BspCNI	CTCAG	6 cut(s)	64, 757, 1436, 1474, 2371, 2572
BspEI	TCCGGA	2 cut(s)	1574, 2656
BspFNI	CGCG	1 cut(s)	382
BspLI	GGNNCC	7 cut(s)	417, 1002, 1476, 1504, 1573, 2421, 3198
BspMI	ACCTGC	1 cut(s)	2869
BspQI	GCTCTTC	1 cut(s)	2503
BspT104I	TTCGAA	2 cut(s)	343, 1392
BspT107I	GGYRCC	1 cut(s)	1502
BsrFI	RCCGGY	1 cut(s)	314
BsrI	ACTGG	8 cut(s)	441, 457, 907, 980, 2438, 2616, 2698, 3078
BssAI	RCCGGY	1 cut(s)	314
BssECI	CCNNGG	6 cut(s)	460, 730, 831, 2423, 3010, 3066
BssNI	GRCGYC	1 cut(s)	102
BssT1I	CCWWGG	2 cut(s)	831, 3010
Bst2UI	CCWGG	9 cut(s)	462, 731, 927, 1648, 1747, 1846, 2014, 2113, 2424
Bst4CI	ACNGT	4 cut(s)	1498, 2231, 2431, 3018
Bst6I	CTCTTC	2 cut(s)	470, 2503
BstACI	GRCGYC	1 cut(s)	102
BstBI	TTCGAA	2 cut(s)	343, 1392
BstC8I	GCNNGC	5 cut(s)	182, 813, 891, 2275, 2586
BstDEI	CTNAG	8 cut(s)	51, 765, 849, 1423, 1461, 2046, 2379, 2580
BstDSI	CCRYGG	1 cut(s)	3010
BstENI	CCTNNNNNAGG	1 cut(s)	269
BstFNI	CGCG	1 cut(s)	382
BstMAI	GTCTC	8 cut(s)	115, 729, 1824, 1992, 2091, 2228, 2654, 2820
BstMWI	GCNNNNNNNGC	8 cut(s)	158, 281, 704, 713, 1076, 2561, 2808, 2817
BstNI	CCWGG	9 cut(s)	462, 731, 927, 1648, 1747, 1846, 2014, 2113, 2424
BstNSI	RCATGY	1 cut(s)	2833
BstSFI	CTRYAG	4 cut(s)	201, 702, 1497, 2911
BstSLI	GKGCMC	1 cut(s)	3129
BstUI	CGCG	1 cut(s)	382
BstV1I	GCAGC	6 cut(s)	58, 173, 238, 775, 2542, 2641
BsuI	GTATCC	1 cut(s)	2397
BsuRI	GGCC	5 cut(s)	117, 459, 1341, 2148, 2811
BtgI	CCRYGG	1 cut(s)	3010
BtsIMutI	CAGTG	3 cut(s)	1494, 2236, 2376
BveI	ACCTGC	1 cut(s)	2869
Cac8I	GCNNGC	5 cut(s)	182, 813, 891, 2275, 2586
CaiI	CAGNNNCTG	2 cut(s)	1232, 2861
Cfr10I	RCCGGY	1 cut(s)	314
Cfr13I	GGNCC	3 cut(s)	458, 2614, 3197
CseI	GACGC	1 cut(s)	91
Csp6I	GTAC	6 cut(s)	34, 278, 2609, 2644, 3167, 3203
CspAI	ACCGGT	1 cut(s)	314
CviQI	GTAC	6 cut(s)	34, 278, 2609, 2644, 3167, 3203
DdeI	CTNAG	8 cut(s)	51, 765, 849, 1423, 1461, 2046, 2379, 2580
Eam1104I	CTCTTC	2 cut(s)	470, 2503
EarI	CTCTTC	2 cut(s)	470, 2503
EciI	GGCGGA	1 cut(s)	3093
Eco130I	CCWWGG	2 cut(s)	831, 3010
Eco147I	AGGCCT	1 cut(s)	1341
Eco47I	GGWCC	2 cut(s)	2614, 3197
Eco57I	CTGAAG	3 cut(s)	837, 2653, 3191
Eco88I	CYCGRG	2 cut(s)	266, 2502
EcoNI	CCTNNNNNAGG	1 cut(s)	269
EcoO109I	RGGNCCY	1 cut(s)	3197
EcoRI	GAATTC	1 cut(s)	2965
EcoRII	CCWGG	9 cut(s)	460, 729, 925, 1646, 1745, 1844, 2012, 2111, 2422
EcoT14I	CCWWGG	2 cut(s)	831, 3010
ErhI	CCWWGG	2 cut(s)	831, 3010
Esp3I	CGTCTC	1 cut(s)	115
FalI	AAGNNNNNCTT	4 cut(s)	240, 272, 670, 702
FaqI	GGGAC	2 cut(s)	2440, 3210
FauNDI	CATATG	2 cut(s)	2449, 2733
FbaI	TGATCA	2 cut(s)	895, 1155
Fnu4HI	GCNGC	8 cut(s)	5, 47, 162, 252, 380, 789, 2556, 2630
Fsp4HI	GCNGC	8 cut(s)	5, 47, 162, 252, 380, 789, 2556, 2630
FspBI	CTAG	5 cut(s)	210, 555, 2589, 2985, 2996
GluI	GCNGC	8 cut(s)	5, 47, 162, 252, 380, 789, 2556, 2630
GsuI	CTGGAG	1 cut(s)	1695
HaeIII	GGCC	5 cut(s)	117, 459, 1341, 2148, 2811
HapII	CCGG	5 cut(s)	315, 1080, 1575, 2657, 3068
HgaI	GACGC	1 cut(s)	91
Hin1I	GRCGYC	1 cut(s)	102
HincII	GTYRAC	3 cut(s)	973, 1013, 2951
HindII	GTYRAC	3 cut(s)	973, 1013, 2951
HindIII	AAGCTT	1 cut(s)	2393
HpaI	GTTAAC	1 cut(s)	973
HpaII	CCGG	5 cut(s)	315, 1080, 1575, 2657, 3068
HphI	GGTGA	8 cut(s)	22, 831, 926, 1565, 2176, 2364, 2420, 2709
Hpy166II	GTNNAC	7 cut(s)	70, 973, 1013, 1187, 2609, 2951, 3127
Hpy8I	GTNNAC	7 cut(s)	70, 973, 1013, 1187, 2609, 2951, 3127
Hpy99I	CGWCG	2 cut(s)	101, 107
HpyCH4III	ACNGT	4 cut(s)	1498, 2231, 2431, 3018
HpyCH4IV	ACGT	2 cut(s)	424, 595
HpyF10VI	GCNNNNNNNGC	8 cut(s)	158, 281, 704, 713, 1076, 2561, 2808, 2817
HpyF3I	CTNAG	8 cut(s)	51, 765, 849, 1423, 1461, 2046, 2379, 2580
HpySE526I	ACGT	2 cut(s)	424, 595
Hsp92I	GRCGYC	1 cut(s)	102
Kpn2I	TCCGGA	2 cut(s)	1574, 2656
Ksp22I	TGATCA	2 cut(s)	895, 1155
KspAI	GTTAAC	1 cut(s)	973
LguI	GCTCTTC	1 cut(s)	2503
LmnI	GCTCC	5 cut(s)	1082, 1577, 1650, 1676, 2312
Lsp1109I	GCAGC	6 cut(s)	58, 173, 238, 775, 2542, 2641
MaeI	CTAG	5 cut(s)	210, 555, 2589, 2985, 2996
MaeII	ACGT	2 cut(s)	424, 595
MaeIII	GTNAC	5 cut(s)	949, 1088, 2225, 2526, 2715
MfeI	CAATTG	1 cut(s)	137
MhlI	GDGCHC	2 cut(s)	96, 3129
MlyI	GAGTC	5 cut(s)	1447, 1826, 1994, 2093, 2969
MmeI	TCCRAC	3 cut(s)	515, 821, 1073
MroI	TCCGGA	2 cut(s)	1574, 2656
MroXI	GAANNNNTTC	1 cut(s)	2475
MseI	TTAA	3 cut(s)	719, 972, 1212
MslI	CAYNNNNRTG	1 cut(s)	2242
MspA1I	CMGCKG	1 cut(s)	7
MspI	CCGG	5 cut(s)	315, 1080, 1575, 2657, 3068
MunI	CAATTG	1 cut(s)	137
Mva1269I	GAATGC	3 cut(s)	651, 2249, 2275
MvaI	CCWGG	9 cut(s)	462, 731, 927, 1648, 1747, 1846, 2014, 2113, 2424
MvnI	CGCG	1 cut(s)	382
MwoI	GCNNNNNNNGC	8 cut(s)	158, 281, 704, 713, 1076, 2561, 2808, 2817
NciI	CCSGG	1 cut(s)	3068
NcoI	CCATGG	1 cut(s)	3010
NdeI	CATATG	2 cut(s)	2449, 2733
NlaIV	GGNNCC	7 cut(s)	417, 1002, 1476, 1504, 1573, 2421, 3198
NmeAIII	GCCGAG	1 cut(s)	178
NmuCI	GTSAC	2 cut(s)	2225, 2715
NspI	RCATGY	1 cut(s)	2833
NspV	TTCGAA	2 cut(s)	343, 1392
PaeR7I	CTCGAG	1 cut(s)	266
PceI	AGGCCT	1 cut(s)	1341
PciI	ACATGT	1 cut(s)	2829
PciSI	GCTCTTC	1 cut(s)	2503
PcsI	WCGNNNNNNNCGW	1 cut(s)	105
PctI	GAATGC	3 cut(s)	651, 2249, 2275
PdmI	GAANNNNTTC	1 cut(s)	2475
PflFI	GACNNNGTC	1 cut(s)	101
PfoI	TCCNGGA	6 cut(s)	925, 1646, 1745, 1844, 2012, 2111
PinAI	ACCGGT	1 cut(s)	314
PkrI	GCNGC	8 cut(s)	6, 48, 163, 253, 381, 790, 2557, 2631
PleI	GAGTC	5 cut(s)	1446, 1825, 1993, 2092, 2968
PpsI	GAGTC	5 cut(s)	1446, 1825, 1993, 2092, 2968
PpuMI	RGGWCCY	1 cut(s)	3197
PscI	ACATGT	1 cut(s)	2829
Psp5II	RGGWCCY	1 cut(s)	3197
Psp6I	CCWGG	9 cut(s)	460, 729, 925, 1646, 1745, 1844, 2012, 2111, 2422
PspGI	CCWGG	9 cut(s)	460, 729, 925, 1646, 1745, 1844, 2012, 2111, 2422
PspN4I	GGNNCC	7 cut(s)	417, 1002, 1476, 1504, 1573, 2421, 3198
PspPI	GGNCC	3 cut(s)	458, 2614, 3197
PspPPI	RGGWCCY	1 cut(s)	3197
PstNI	CAGNNNCTG	2 cut(s)	1232, 2861
PsyI	GACNNNGTC	1 cut(s)	101
RsaI	GTAC	6 cut(s)	35, 279, 2610, 2645, 3168, 3204
RsaNI	GTAC	6 cut(s)	34, 278, 2609, 2644, 3167, 3203
RseI	CAYNNNNRTG	1 cut(s)	2242
SapI	GCTCTTC	1 cut(s)	2503
SaqAI	TTAA	3 cut(s)	719, 972, 1212
SatI	GCNGC	8 cut(s)	5, 47, 162, 252, 380, 789, 2556, 2630
Sau96I	GGNCC	3 cut(s)	458, 2614, 3197
SchI	GAGTC	5 cut(s)	1447, 1826, 1994, 2093, 2969
SduI	GDGCHC	2 cut(s)	96, 3129
SfcI	CTRYAG	4 cut(s)	201, 702, 1497, 2911
Sfr274I	CTCGAG	1 cut(s)	266
SfuI	TTCGAA	2 cut(s)	343, 1392
SgrAI	CRCCGGYG	1 cut(s)	314
SinI	GGWCC	2 cut(s)	2614, 3197
SlaI	CTCGAG	1 cut(s)	266
SmiMI	CAYNNNNRTG	1 cut(s)	2242
SmlI	CTYRAG	7 cut(s)	266, 1623, 1722, 1821, 1989, 2088, 2159
SmoI	CTYRAG	7 cut(s)	266, 1623, 1722, 1821, 1989, 2088, 2159
SseBI	AGGCCT	1 cut(s)	1341
SsiI	CCGC	4 cut(s)	5, 25, 380, 3078
SspMI	CTAG	5 cut(s)	210, 555, 2589, 2985, 2996
StuI	AGGCCT	1 cut(s)	1341
StyI	CCWWGG	2 cut(s)	831, 3010
TaaI	ACNGT	4 cut(s)	1498, 2231, 2431, 3018
TaiI	ACGT	2 cut(s)	427, 598
TaqI	TCGA	7 cut(s)	96, 267, 343, 1329, 1392, 2963, 3186
TatI	WGTACW	5 cut(s)	33, 277, 2608, 3166, 3202
TauI	GCSGC	2 cut(s)	7, 382
Tru1I	TTAA	3 cut(s)	719, 972, 1212
Tru9I	TTAA	3 cut(s)	719, 972, 1212
TscAI	CASTG	3 cut(s)	1501, 2236, 2383
TseFI	GTSAC	2 cut(s)	2225, 2715
TseI	GCWGC	6 cut(s)	46, 161, 251, 788, 2555, 2629
Tsp45I	GTSAC	2 cut(s)	2225, 2715
TspGWI	ACGGA	2 cut(s)	2379, 2589
TspRI	CASTG	3 cut(s)	1501, 2236, 2383
Tth111I	GACNNNGTC	1 cut(s)	101
VneI	GTGCAC	1 cut(s)	3125
VpaK11BI	GGWCC	2 cut(s)	2614, 3197
XagI	CCTNNNNNAGG	1 cut(s)	269
XapI	RAATTY	5 cut(s)	1361, 2007, 2128, 2879, 2965
XbaI	TCTAGA	2 cut(s)	209, 2995
XceI	RCATGY	1 cut(s)	2833
XhoI	CTCGAG	1 cut(s)	266
XmnI	GAANNNNTTC	1 cut(s)	2475
XspI	CTAG	5 cut(s)	210, 555, 2589, 2985, 2996

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.