MD05G1301800.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004530
Physical Location & Seq
Forward (+)
43441063 .. 43441470
408 bp
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UTR
Exon/CDS
Intron
MD05G1301800.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGCGGAGAAGGAAGGAGGAATCGTGAAGAAAGGCCACGAGGAGGGACTGAGATTGGCTACCGCCTTGCTCCAAGAGTTTGAACTTCCGCTAGGGCTTTTGCCTCTTGCTGATGTGATCGAAGTTGGCTTTGTGAGAGCCACCGGCTACATGTGGATCGTCCAGAAGAAGAAGGTCGAACACAGCTTCAAGTTGATCAGTAAGCTTGTGAGTTATGACACTGAAATCAAAGGCTATGTTGAGAAGCAAAAGATTAAGAAGCTCAAGGGAGTGAAGGCTAAGGAGCTTATGCTCTGGCCTCCGGTTAGTGAAATAATCGTTGATCAGCCTGCCGGAAAAATCAACTTCAAGAGTCTTGCTGGTATCACCAAGACTTTCCCCGTTGAGGCATTTGCTGCTGGGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

136

Amino Acids

14.97

Weight (kDa)

9.35

Isoelectric Point (pI)

28.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 21 - 132 1.2e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 63, 89
AclWI GGATC 1 cut(s) 164
AfiI CCNNNNNNNGG 2 cut(s) 43, 385
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 3 cut(s) 83, 190, 349
AluBI AGCT 4 cut(s) 186, 205, 262, 286
AluI AGCT 4 cut(s) 186, 205, 262, 286
AlwI GGATC 1 cut(s) 164
AoxI GGCC 2 cut(s) 34, 296
ApeKI GCWGC 1 cut(s) 395
AsuHPI GGTGA 1 cut(s) 358
BauI CACGAG 1 cut(s) 38
BbvI GCAGC 1 cut(s) 382
BclI TGATCA 2 cut(s) 195, 322
BfaI CTAG 1 cut(s) 92
BisI GCNGC 1 cut(s) 396
BlsI GCNGC 1 cut(s) 397
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 248
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 385
Bse118I RCCGGY 1 cut(s) 143
BseLI CCNNNNNNNGG 2 cut(s) 43, 385
BseMII CTCAG 1 cut(s) 41
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 1 cut(s) 382
BseYI CCCAGC 1 cut(s) 398
BshFI GGCC 2 cut(s) 36, 298
BsiSI CCGG 3 cut(s) 144, 302, 333
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 43, 385
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 2 cut(s) 36, 298
Bsp143I GATC 4 cut(s) 117, 156, 195, 322
BspACI CCGC 3 cut(s) 5, 63, 89
BspANI GGCC 2 cut(s) 36, 298
BspCNI CTCAG 1 cut(s) 42
BspPI GGATC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 143
BssAI RCCGGY 1 cut(s) 143
BssMI GATC 4 cut(s) 117, 156, 195, 322
BssSI CACGAG 1 cut(s) 38
Bst2BI CACGAG 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 2 cut(s) 50, 279
BstKTI GATC 4 cut(s) 120, 159, 198, 325
BstMBI GATC 4 cut(s) 117, 156, 195, 322
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNSI RCATGY 1 cut(s) 154
BstV1I GCAGC 1 cut(s) 382
BsuRI GGCC 2 cut(s) 36, 298
BtsIMutI CAGTG 1 cut(s) 219
Cac8I GCNNGC 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 143
CviAII CATG 1 cut(s) 151
DdeI CTNAG 2 cut(s) 50, 279
DpnI GATC 4 cut(s) 119, 158, 197, 324
DpnII GATC 4 cut(s) 117, 156, 195, 322
EciI GGCGGA 1 cut(s) 20
FaeI CATG 1 cut(s) 154
FaiI YATR 4 cut(s) 152, 216, 237, 290
FaqI GGGAC 1 cut(s) 60
FatI CATG 1 cut(s) 150
FbaI TGATCA 2 cut(s) 195, 322
Fnu4HI GCNGC 1 cut(s) 396
Fsp4HI GCNGC 1 cut(s) 396
FspBI CTAG 1 cut(s) 92
GluI GCNGC 1 cut(s) 396
GsaI CCCAGC 1 cut(s) 402
HaeIII GGCC 2 cut(s) 36, 298
HapII CCGG 3 cut(s) 144, 302, 333
Hin1II CATG 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 2 cut(s) 21, 352
HpaII CCGG 3 cut(s) 144, 302, 333
HphI GGTGA 1 cut(s) 358
Hpy188III TCNNGA 3 cut(s) 25, 163, 349
HpyAV CCTTC 4 cut(s) 4, 8, 166, 268
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 2 cut(s) 50, 279
Hsp92II CATG 1 cut(s) 154
Ksp22I TGATCA 2 cut(s) 195, 322
Kzo9I GATC 4 cut(s) 117, 156, 195, 322
LmnI GCTCC 2 cut(s) 75, 283
LpnPI CCDG 8 cut(s) 157, 176, 280, 315, 342, 345, 346, 384
Lsp1109I GCAGC 1 cut(s) 382
MaeI CTAG 1 cut(s) 92
MalI GATC 4 cut(s) 119, 158, 197, 324
MboI GATC 4 cut(s) 117, 156, 195, 322
MboII GAAGA 3 cut(s) 40, 178, 181
MlyI GAGTC 1 cut(s) 361
MnlI CCTC 6 cut(s) 11, 34, 37, 114, 309, 379
MseI TTAA 1 cut(s) 255
MspI CCGG 3 cut(s) 144, 302, 333
MwoI GCNNNNNNNGC 1 cut(s) 395
NdeII GATC 4 cut(s) 117, 156, 195, 322
NlaIII CATG 1 cut(s) 154
NspI RCATGY 1 cut(s) 154
PciI ACATGT 1 cut(s) 150
PfeI GAWTC 1 cut(s) 21
PkrI GCNGC 1 cut(s) 397
PleI GAGTC 1 cut(s) 360
PpsI GAGTC 1 cut(s) 360
PscI ACATGT 1 cut(s) 150
PspFI CCCAGC 1 cut(s) 398
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 1 cut(s) 396
Sau3AI GATC 4 cut(s) 117, 156, 195, 322
SchI GAGTC 1 cut(s) 361
SetI ASST 5 cut(s) 177, 188, 207, 264, 288
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SsiI CCGC 3 cut(s) 5, 63, 89
SspMI CTAG 1 cut(s) 92
TaqI TCGA 2 cut(s) 120, 177
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 1 cut(s) 226
TseI GCWGC 1 cut(s) 395
TspRI CASTG 1 cut(s) 226
XceI RCATGY 1 cut(s) 154
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.