MD10G1280700.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004530
Physical Location & Seq
Forward (+)
37136307 .. 37136714
408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1280700.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGCCGAGAAGGAAGGAGGAATCGTGAAGAAGGGCCACGAGGCGGGACTGGCATTGGCGACCGCCTTGCTCCAAGAGTTTGAACTTCCGCTAGGGCTTTTGCCTCTTGCTGATGTGGTCGAAGTTGGCTTTGTGAGGACCACCGGCTACATGTGGATCCTCCAGAAGAAGAAGGTTGAACACAGCTTCAAGTTGATCGGTAAGCTTGTGAGCTATGACACTGAAATCAAAGGCTATATTGAGAAGCAGAAGATTAAGAAGCTCAAGGGAGTTAAGGCTAAGGAGCTCATGCTATGGCCTCCGGTCAGTGAAATAAATGTGGATCAGGCCGCCGGAAAAATCAACTTCAAGAGCCTTGCCGGCATCACCAAGACTTTCCCCGTTGAGGCATTTGCTGCTGGCCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

136

Amino Acids

14.81

Weight (kDa)

9.35

Isoelectric Point (pI)

26.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 21 - 132 7.3e-34 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 44, 63, 89, 330
AclWI GGATC 3 cut(s) 151, 164, 330
AcoI YGGCCR 2 cut(s) 3, 400
AfiI CCNNNNNNNGG 2 cut(s) 43, 385
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 83, 179, 190, 349
AluBI AGCT 5 cut(s) 186, 205, 213, 262, 286
AluI AGCT 5 cut(s) 186, 205, 213, 262, 286
Alw21I GWGCWC 1 cut(s) 288
AlwI GGATC 3 cut(s) 151, 164, 330
AoxI GGCC 5 cut(s) 3, 34, 296, 327, 400
ApeKI GCWGC 1 cut(s) 395
AspS9I GGNCC 2 cut(s) 34, 138
AsuHPI GGTGA 1 cut(s) 358
AvaII GGWCC 1 cut(s) 138
BalI TGGCCA 1 cut(s) 402
BamHI GGATCC 1 cut(s) 156
BanII GRGCYC 1 cut(s) 288
BauI CACGAG 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 1 cut(s) 382
BcgI CGANNNNNNTGC 2 cut(s) 49, 83
BfaI CTAG 1 cut(s) 92
BglI GCCNNNNNGGC 1 cut(s) 360
BisI GCNGC 2 cut(s) 330, 396
BlsI GCNGC 2 cut(s) 331, 397
Bme18I GGWCC 1 cut(s) 138
BmgT120I GGNCC 2 cut(s) 34, 138
BmiI GGNNCC 1 cut(s) 158
BmsI GCATC 1 cut(s) 372
BpmI CTGGAG 1 cut(s) 146
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 248
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 385
Bse118I RCCGGY 2 cut(s) 143, 359
Bse1I ACTGG 2 cut(s) 54, 403
BseLI CCNNNNNNNGG 2 cut(s) 43, 385
BseNI ACTGG 2 cut(s) 54, 403
BseXI GCAGC 1 cut(s) 382
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 5 cut(s) 5, 36, 298, 329, 402
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 4 cut(s) 144, 302, 333, 360
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 43, 385
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 5 cut(s) 5, 36, 298, 329, 402
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 3 cut(s) 156, 195, 322
BspACI CCGC 4 cut(s) 44, 63, 89, 330
BspANI GGCC 5 cut(s) 5, 36, 298, 329, 402
BspLI GGNNCC 1 cut(s) 158
BspPI GGATC 3 cut(s) 151, 164, 330
BsrFI RCCGGY 2 cut(s) 143, 359
BsrI ACTGG 2 cut(s) 54, 403
BssAI RCCGGY 2 cut(s) 143, 359
BssMI GATC 3 cut(s) 156, 195, 322
BssSI CACGAG 1 cut(s) 38
Bst2BI CACGAG 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 2 cut(s) 361, 400
BstDEI CTNAG 1 cut(s) 279
BstKTI GATC 3 cut(s) 159, 198, 325
BstMBI GATC 3 cut(s) 156, 195, 322
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 3 cut(s) 50, 360, 395
BstNSI RCATGY 1 cut(s) 154
BstV1I GCAGC 1 cut(s) 382
BstX2I RGATCY 1 cut(s) 156
BstYI RGATCY 1 cut(s) 156
BsuRI GGCC 5 cut(s) 5, 36, 298, 329, 402
BtsIMutI CAGTG 2 cut(s) 219, 313
Cac8I GCNNGC 2 cut(s) 361, 400
Cfr10I RCCGGY 2 cut(s) 143, 359
Cfr13I GGNCC 2 cut(s) 34, 138
CviAII CATG 2 cut(s) 151, 289
DdeI CTNAG 1 cut(s) 279
DpnI GATC 3 cut(s) 158, 197, 324
DpnII GATC 3 cut(s) 156, 195, 322
EaeI YGGCCR 2 cut(s) 3, 400
Ecl136II GAGCTC 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 286
EcoICRI GAGCTC 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 288
FaeI CATG 2 cut(s) 154, 292
FaiI YATR 5 cut(s) 152, 216, 237, 290, 295
FaqI GGGAC 1 cut(s) 60
FatI CATG 2 cut(s) 150, 288
FauI CCCGC 1 cut(s) 37
Fnu4HI GCNGC 2 cut(s) 330, 396
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 2 cut(s) 330, 396
FspBI CTAG 1 cut(s) 92
GluI GCNGC 2 cut(s) 330, 396
GsuI CTGGAG 1 cut(s) 146
HaeIII GGCC 5 cut(s) 5, 36, 298, 329, 402
HapII CCGG 4 cut(s) 144, 302, 333, 360
Hin1II CATG 2 cut(s) 154, 292
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 1 cut(s) 21
HpaII CCGG 4 cut(s) 144, 302, 333, 360
HphI GGTGA 1 cut(s) 358
Hpy188III TCNNGA 3 cut(s) 25, 163, 349
HpyAV CCTTC 4 cut(s) 4, 8, 25, 166
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 360, 395
HpyF3I CTNAG 1 cut(s) 279
Hsp92II CATG 2 cut(s) 154, 292
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Kzo9I GATC 3 cut(s) 156, 195, 322
LmnI GCTCC 2 cut(s) 75, 283
LpnPI CCDG 8 cut(s) 35, 157, 176, 311, 315, 346, 373, 384
Lsp1109I GCAGC 1 cut(s) 382
LweI GCATC 1 cut(s) 372
MaeI CTAG 1 cut(s) 92
MalI GATC 3 cut(s) 158, 197, 324
MboI GATC 3 cut(s) 156, 195, 322
MboII GAAGA 4 cut(s) 40, 178, 181, 262
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 1 cut(s) 288
MlsI TGGCCA 1 cut(s) 402
MluNI TGGCCA 1 cut(s) 402
MnlI CCTC 7 cut(s) 11, 34, 114, 129, 170, 309, 379
Mox20I TGGCCA 1 cut(s) 402
MroNI GCCGGC 1 cut(s) 359
MscI TGGCCA 1 cut(s) 402
MseI TTAA 2 cut(s) 255, 273
Msp20I TGGCCA 1 cut(s) 402
MspI CCGG 4 cut(s) 144, 302, 333, 360
MwoI GCNNNNNNNGC 3 cut(s) 50, 360, 395
NaeI GCCGGC 1 cut(s) 361
NdeII GATC 3 cut(s) 156, 195, 322
NgoMIV GCCGGC 1 cut(s) 359
NlaIII CATG 2 cut(s) 154, 292
NlaIV GGNNCC 1 cut(s) 158
NmeAIII GCCGAG 1 cut(s) 31
NspI RCATGY 1 cut(s) 154
PciI ACATGT 1 cut(s) 150
PdiI GCCGGC 1 cut(s) 361
PfeI GAWTC 1 cut(s) 21
PkrI GCNGC 2 cut(s) 331, 397
PscI ACATGT 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 288
PspN4I GGNNCC 1 cut(s) 158
PspPI GGNCC 2 cut(s) 34, 138
PsuI RGATCY 1 cut(s) 156
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 255, 273
SatI GCNGC 2 cut(s) 330, 396
Sau3AI GATC 3 cut(s) 156, 195, 322
Sau96I GGNCC 2 cut(s) 34, 138
SduI GDGCHC 1 cut(s) 288
SetI ASST 6 cut(s) 177, 188, 207, 215, 264, 288
SfaNI GCATC 1 cut(s) 372
SinI GGWCC 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SsiI CCGC 4 cut(s) 44, 63, 89, 330
SspMI CTAG 1 cut(s) 92
SstI GAGCTC 1 cut(s) 288
TaqI TCGA 1 cut(s) 120
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 2 cut(s) 255, 273
Tru9I TTAA 2 cut(s) 255, 273
TscAI CASTG 2 cut(s) 226, 313
TseI GCWGC 1 cut(s) 395
TspRI CASTG 2 cut(s) 226, 313
VpaK11BI GGWCC 1 cut(s) 138
XceI RCATGY 1 cut(s) 154
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.