MD10G1280600.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004530
Physical Location & Seq
Reverse (-)
37135375 .. 37135782
408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1280600.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGCGGAGAAGGAAGGAGGAATCGTGAAGAAAGGCCACGAGGAGGGACTGGCATTGGCGACCGCCTTGCTCAAGGAGTTTGAACTTCCGCTAGGGCTTTTGCCACTTGCTGATGTGGTCGAAGTTGGCTTTGTGAGGACCACCGGCTACATGTGGATCGTCCAGAAGAAGAAGGTCGAGCACAGCTTCAAGTTGATCGGTAAGCTTGTGAGTTACGACACTGAAATCAAGGGCTATATTGAGAAGCAGAGGATTAAGAAGCTCAAGGGAGTGAAGGCTAAGGAGCTCATGCTATGGCCTCCCGTCAGTGAAATAATTGTTGATCAGCCCGCCAGAAAAATCAACTTCAAGAGCCTTGCCGGTATCACCAAGACTTTCCCGGTCGAGGCATTCGCTGCCGGGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

136

Amino Acids

15.01

Weight (kDa)

9.47

Isoelectric Point (pI)

22.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 21 - 132 3.4e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 5, 63, 89, 330
AclWI GGATC 1 cut(s) 164
AfiI CCNNNNNNNGG 2 cut(s) 43, 385
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 3 cut(s) 83, 190, 349
AluBI AGCT 4 cut(s) 186, 205, 262, 286
AluI AGCT 4 cut(s) 186, 205, 262, 286
Alw21I GWGCWC 2 cut(s) 183, 288
AlwI GGATC 1 cut(s) 164
AoxI GGCC 2 cut(s) 34, 296
ApeKI GCWGC 1 cut(s) 395
AspS9I GGNCC 1 cut(s) 138
AsuC2I CCSGG 2 cut(s) 380, 400
AsuHPI GGTGA 1 cut(s) 358
AvaII GGWCC 1 cut(s) 138
BanII GRGCYC 1 cut(s) 288
BauI CACGAG 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 183, 288
BbvI GCAGC 1 cut(s) 382
BcgI CGANNNNNNTGC 2 cut(s) 49, 83
BclI TGATCA 1 cut(s) 322
BcnI CCSGG 2 cut(s) 380, 400
BfaI CTAG 1 cut(s) 92
BisI GCNGC 1 cut(s) 396
BlsI GCNGC 1 cut(s) 397
Bme1390I CCNGG 2 cut(s) 380, 400
Bme18I GGWCC 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 138
BmrFI CCNGG 2 cut(s) 380, 400
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 2 cut(s) 56, 248
BpuMI CCSGG 2 cut(s) 380, 400
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 385
Bse118I RCCGGY 2 cut(s) 143, 359
Bse1I ACTGG 1 cut(s) 54
BseLI CCNNNNNNNGG 2 cut(s) 43, 385
BseNI ACTGG 1 cut(s) 54
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 1 cut(s) 382
Bsh1285I CGRYCG 2 cut(s) 63, 384
BshFI GGCC 2 cut(s) 36, 298
BsiEI CGRYCG 2 cut(s) 63, 384
BsiHKAI GWGCWC 2 cut(s) 183, 288
BsiSI CCGG 4 cut(s) 144, 360, 380, 399
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 2 cut(s) 43, 385
BsmFI GGGAC 1 cut(s) 60
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 2 cut(s) 36, 298
Bsp1286I GDGCHC 2 cut(s) 183, 288
Bsp143I GATC 3 cut(s) 156, 195, 322
BspACI CCGC 4 cut(s) 5, 63, 89, 330
BspANI GGCC 2 cut(s) 36, 298
BspPI GGATC 1 cut(s) 164
BsrFI RCCGGY 2 cut(s) 143, 359
BsrI ACTGG 1 cut(s) 54
BssAI RCCGGY 2 cut(s) 143, 359
BssMI GATC 3 cut(s) 156, 195, 322
BssSI CACGAG 1 cut(s) 38
Bst2BI CACGAG 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 1 cut(s) 279
BstKTI GATC 3 cut(s) 159, 198, 325
BstMBI GATC 3 cut(s) 156, 195, 322
BstMCI CGRYCG 2 cut(s) 63, 384
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 2 cut(s) 378, 398
BstV1I GCAGC 1 cut(s) 382
BsuRI GGCC 2 cut(s) 36, 298
BtsIMutI CAGTG 2 cut(s) 219, 313
Cac8I GCNNGC 1 cut(s) 330
Cfr10I RCCGGY 2 cut(s) 143, 359
Cfr13I GGNCC 1 cut(s) 138
CviAII CATG 2 cut(s) 151, 289
DdeI CTNAG 1 cut(s) 279
DpnI GATC 3 cut(s) 158, 197, 324
DpnII GATC 3 cut(s) 156, 195, 322
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 286
EcoICRI GAGCTC 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 288
FaeI CATG 2 cut(s) 154, 292
FaiI YATR 4 cut(s) 152, 237, 290, 295
FaqI GGGAC 1 cut(s) 60
FatI CATG 2 cut(s) 150, 288
FauI CCCGC 1 cut(s) 337
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 396
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 1 cut(s) 396
FspBI CTAG 1 cut(s) 92
GluI GCNGC 1 cut(s) 396
HaeIII GGCC 2 cut(s) 36, 298
HapII CCGG 4 cut(s) 144, 360, 380, 399
Hin1II CATG 2 cut(s) 154, 292
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 1 cut(s) 21
HpaII CCGG 4 cut(s) 144, 360, 380, 399
HphI GGTGA 1 cut(s) 358
Hpy188III TCNNGA 3 cut(s) 25, 163, 349
HpyAV CCTTC 4 cut(s) 4, 8, 166, 268
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 1 cut(s) 279
Hsp92II CATG 2 cut(s) 154, 292
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 3 cut(s) 156, 195, 322
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 6 cut(s) 35, 157, 176, 346, 373, 393
Lsp1109I GCAGC 1 cut(s) 382
MaeI CTAG 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 212
MalI GATC 3 cut(s) 158, 197, 324
MboI GATC 3 cut(s) 156, 195, 322
MboII GAAGA 3 cut(s) 40, 178, 181
MhlI GDGCHC 2 cut(s) 183, 288
MluCI AATT 1 cut(s) 315
MnlI CCTC 7 cut(s) 11, 34, 37, 129, 243, 309, 379
MseI TTAA 1 cut(s) 255
MspI CCGG 4 cut(s) 144, 360, 380, 399
MspR9I CCNGG 2 cut(s) 380, 400
Mva1269I GAATGC 1 cut(s) 389
MwoI GCNNNNNNNGC 1 cut(s) 395
NciI CCSGG 2 cut(s) 380, 400
NdeII GATC 3 cut(s) 156, 195, 322
NlaIII CATG 2 cut(s) 154, 292
NspI RCATGY 1 cut(s) 154
PciI ACATGT 1 cut(s) 150
PctI GAATGC 1 cut(s) 389
PfeI GAWTC 1 cut(s) 21
PkrI GCNGC 1 cut(s) 397
PscI ACATGT 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 288
PspPI GGNCC 1 cut(s) 138
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 1 cut(s) 396
Sau3AI GATC 3 cut(s) 156, 195, 322
Sau96I GGNCC 1 cut(s) 138
ScrFI CCNGG 2 cut(s) 380, 400
SduI GDGCHC 2 cut(s) 183, 288
SetI ASST 5 cut(s) 177, 188, 207, 264, 288
SinI GGWCC 1 cut(s) 138
SmlI CTYRAG 2 cut(s) 71, 263
SmoI CTYRAG 2 cut(s) 71, 263
Sse9I AATT 1 cut(s) 315
SsiI CCGC 4 cut(s) 5, 63, 89, 330
SspMI CTAG 1 cut(s) 92
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 378, 398
TaqI TCGA 3 cut(s) 120, 177, 384
TasI AATT 1 cut(s) 315
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 2 cut(s) 226, 313
TseI GCWGC 1 cut(s) 395
TspRI CASTG 2 cut(s) 226, 313
VpaK11BI GGWCC 1 cut(s) 138
XceI RCATGY 1 cut(s) 154
XspI CTAG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.