RchiOBHm_Chr5g0010731

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004530
Physical Location & Seq
Reverse (-)
7059024 .. 7060061
1038 bp
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UTR
Exon/CDS
Intron
PRQ29145

Sequence Viewer

Length: 411 bp
ATGGCAGAGAAGGAAGGAGGAATCGTCAAGAAGGGCCACGAAGAGGGACTGGCATTGGCAACTGCACTTCTCCAAGAGTTTGAGCTTCCACTCGGACTTTTGCCCCTCTCTGATGTGATTGAAGTCGGTTTCGTGAGGAGCACTGGCTACATGTGGATTGTCCAGAAGAAGAAGGTTGAGCACAGCTTCAAGATCATCAGTAAGCTTGTGAGTTACGACACAGAAATTAAAGGATATGTTGAAAAGAAGAGGATTAAGAAGCTCAAGGGAGTGAAGGCCAAGGAGCTCATGTTGTGGCCTCCAGTCAGTGAAATCATTGCTGATGAGCAACCCACTGGAAAGATTCACTTCAAGAGCCTTGCTGGCATCACCAAAACTTTTCCAGCTGAGGCATTTGCTGCTGGTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

136

Amino Acids

15.07

Weight (kDa)

9.2

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 21 - 133 3e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 1 cut(s) 43
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 4 cut(s) 122, 190, 242, 352
AluBI AGCT 6 cut(s) 85, 186, 205, 262, 286, 386
AluI AGCT 6 cut(s) 85, 186, 205, 262, 286, 386
Alw21I GWGCWC 3 cut(s) 143, 183, 288
AoxI GGCC 3 cut(s) 34, 276, 296
ApeKI GCWGC 1 cut(s) 398
AspS9I GGNCC 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 361
BanII GRGCYC 1 cut(s) 288
Bbv12I GWGCWC 3 cut(s) 143, 183, 288
BbvCI CCTCAGC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 385
BglI GCCNNNNNGGC 1 cut(s) 363
BisI GCNGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 34
BmsI GCATC 1 cut(s) 375
BpmI CTGGAG 1 cut(s) 285
Bpu10I CCTNAGC 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 248
BsaJI CCNNGG 1 cut(s) 279
BsaXI ACNNNNNCTCC 4 cut(s) 9, 39, 275, 305
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse1I ACTGG 4 cut(s) 54, 148, 302, 340
Bse3DI GCAATG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 4 cut(s) 54, 148, 302, 340
BseRI GAGGAG 1 cut(s) 151
BseXI GCAGC 1 cut(s) 385
BsgI GTGCAG 1 cut(s) 48
BshFI GGCC 3 cut(s) 36, 278, 298
BsiHKAI GWGCWC 3 cut(s) 143, 183, 288
BslFI GGGAC 1 cut(s) 60
BslI CCNNNNNNNGG 1 cut(s) 43
BsmFI GGGAC 1 cut(s) 60
BsnI GGCC 3 cut(s) 36, 278, 298
Bsp1286I GDGCHC 3 cut(s) 143, 183, 288
Bsp143I GATC 1 cut(s) 192
BspANI GGCC 3 cut(s) 36, 278, 298
BspCNI CTCAG 1 cut(s) 379
BsrDI GCAATG 1 cut(s) 315
BsrI ACTGG 4 cut(s) 54, 148, 302, 340
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 279
Bst6I CTCTTC 2 cut(s) 36, 242
BstAPI GCANNNNNTGC 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 364
BstDEI CTNAG 1 cut(s) 387
BstKTI GATC 1 cut(s) 195
BstMBI GATC 1 cut(s) 192
BstMWI GCNNNNNNNGC 2 cut(s) 363, 398
BstNSI RCATGY 1 cut(s) 154
BstV1I GCAGC 1 cut(s) 385
BsuRI GGCC 3 cut(s) 36, 278, 298
BtsIMutI CAGTG 3 cut(s) 141, 313, 333
Cac8I GCNNGC 1 cut(s) 364
Cfr13I GGNCC 1 cut(s) 34
CviAII CATG 2 cut(s) 151, 289
DdeI CTNAG 1 cut(s) 387
DpnI GATC 1 cut(s) 194
DpnII GATC 1 cut(s) 192
Eam1104I CTCTTC 2 cut(s) 36, 242
EarI CTCTTC 2 cut(s) 36, 242
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 286
EcoICRI GAGCTC 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 279
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 2 cut(s) 154, 292
FaiI YATR 3 cut(s) 152, 237, 290
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FaqI GGGAC 1 cut(s) 60
FatI CATG 2 cut(s) 150, 288
Fnu4HI GCNGC 1 cut(s) 399
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 1 cut(s) 399
GluI GCNGC 1 cut(s) 399
GsuI CTGGAG 1 cut(s) 285
HaeIII GGCC 3 cut(s) 36, 278, 298
Hin1II CATG 2 cut(s) 154, 292
HindIII AAGCTT 1 cut(s) 203
HinfI GANTC 2 cut(s) 21, 343
HphI GGTGA 1 cut(s) 361
Hpy188I TCNGA 2 cut(s) 95, 112
Hpy188III TCNNGA 5 cut(s) 28, 133, 163, 190, 352
HpyAV CCTTC 5 cut(s) 4, 8, 25, 166, 268
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 2 cut(s) 363, 398
HpyF3I CTNAG 1 cut(s) 387
Hsp92II CATG 2 cut(s) 154, 292
Kzo9I GATC 1 cut(s) 192
LmnI GCTCC 2 cut(s) 138, 283
LpnPI CCDG 8 cut(s) 35, 129, 176, 315, 321, 348, 387, 396
Lsp1109I GCAGC 1 cut(s) 385
LweI GCATC 1 cut(s) 375
MaeIII GTNAC 1 cut(s) 212
MalI GATC 1 cut(s) 194
MboI GATC 1 cut(s) 192
MboII GAAGA 4 cut(s) 53, 178, 181, 259
MhlI GDGCHC 3 cut(s) 143, 183, 288
MluCI AATT 1 cut(s) 225
MnlI CCTC 7 cut(s) 11, 37, 116, 129, 243, 309, 382
MseI TTAA 2 cut(s) 228, 255
MspA1I CMGCKG 1 cut(s) 386
MwoI GCNNNNNNNGC 2 cut(s) 363, 398
NdeII GATC 1 cut(s) 192
NlaIII CATG 2 cut(s) 154, 292
NspI RCATGY 1 cut(s) 154
PciI ACATGT 1 cut(s) 150
PfeI GAWTC 2 cut(s) 21, 343
PkrI GCNGC 1 cut(s) 400
PscI ACATGT 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 288
PspPI GGNCC 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 386
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 228, 255
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 1 cut(s) 192
Sau96I GGNCC 1 cut(s) 34
SduI GDGCHC 3 cut(s) 143, 183, 288
SetI ASST 7 cut(s) 87, 177, 188, 207, 264, 288, 388
SfaNI GCATC 1 cut(s) 375
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 1 cut(s) 225
SstI GAGCTC 1 cut(s) 288
StyI CCWWGG 1 cut(s) 279
TasI AATT 1 cut(s) 225
TfiI GAWTC 2 cut(s) 21, 343
Tru1I TTAA 2 cut(s) 228, 255
Tru9I TTAA 2 cut(s) 228, 255
TscAI CASTG 3 cut(s) 148, 313, 340
TseI GCWGC 1 cut(s) 398
TspRI CASTG 3 cut(s) 148, 313, 340
XceI RCATGY 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.