MD05G1312600.v1.1

50S ribosomal protein L21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006560
Physical Location & Seq
Forward (+)
44322968 .. 44326186
3219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1312600.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGCGACCCGACGGCGCCTCCAAGCTCTGACCCGCCACGCCTCCGCCCTCTTCTCCAAAAGCCCTTCATTCCACTCCCTCCCAGCTCCCCTCCCCATCTACCAACCCCTCAAAACCCTCACCTCCAATTTCTCCCTCATTTCCTCCAGCCCCGATCCCACTCGCTCACTTACCCGCCATTTCTCGTCGAAACCCCGAATCGGCGACGTAAATGAAGACAATGAGCACAGTGAAGAGGAGGAAGACGACGACGACGACGACGATGACGACGATGAAGAAGTTGGGGACAGTGAGGACGAGAGTGCTTACCGATGCAGCGGTTCGAAAAGAGAGTATACTCCAGAGGAGAAAGAGGCGGAGGCCGCCGGCATTGGGTACCAAGTGATTGGGCCGCTTCAGAGGTCTGATCGGGTTTTCAAGCCATATGAGCCGGTTTTCGCGGTGGTTCAGGTTGGTTCACATCAGTTCAAGGTTAGCAACGGGGACTGCATTTTCACGGAAAAGTTGAAATTCTGCGAAGTGAATGATAAGTTAGTACTGAACAAGGTTCTCATGCTGGGCTCAAGTAGTCAGACAATAATTGGTAGGCCCATATTACCAGATGCAGCAGTTCATGCGGTTGTTGAGGAGCACGCATTAGATGCAAAGGTAATTATTTTTAAGAAAAAGAGGAGGAAGAATTACCGTCGAACCAAAGGACATCGCCAGGAATTGACTAAGTTGAGGATAGTTGATGTTCAAGGAATTGAGAAACCAGAGCCTGTAGTCACTGAAAAGCCTCCAAAGGCTCCAAAGGCACCTGGTAAGAAAACGGAAAAGGTTGTGGTTGCTGCTTAG

Protein Analysis

279

Amino Acids

31.09

Weight (kDa)

6.28

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L21p PF00829 146 - 245 1.2e-31 Ribosomal prokaryotic L21 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 375
AccB1I GGYRCC 3 cut(s) 15, 375, 796
AccI GTMKAC 1 cut(s) 335
AccII CGCG 1 cut(s) 440
AciI CCGC 9 cut(s) 34, 45, 175, 318, 356, 363, 392, 440, 617
AclWI GGATC 1 cut(s) 149
AcsI RAATTY 1 cut(s) 509
AcuI CTGAAG 1 cut(s) 380
AcyI GRCGYC 1 cut(s) 16
AfaI GTAC 2 cut(s) 377, 537
AfiI CCNNNNNNNGG 2 cut(s) 200, 372
AgsI TTSAA 4 cut(s) 418, 469, 508, 740
AjnI CCWGG 2 cut(s) 705, 799
AluBI AGCT 2 cut(s) 26, 86
AluI AGCT 2 cut(s) 26, 86
Alw21I GWGCWC 2 cut(s) 228, 633
AlwI GGATC 1 cut(s) 149
AlwNI CAGNNNCTG 1 cut(s) 761
AoxI GGCC 3 cut(s) 360, 389, 587
ApeKI GCWGC 3 cut(s) 315, 605, 830
ApoI RAATTY 1 cut(s) 509
Asp718I GGTACC 1 cut(s) 375
AspLEI GCGC 1 cut(s) 18
AspS9I GGNCC 2 cut(s) 389, 588
AsuHPI GGTGA 1 cut(s) 112
AsuII TTCGAA 1 cut(s) 323
BanI GGYRCC 3 cut(s) 15, 375, 796
BanII GRGCYC 1 cut(s) 563
BbsI GAAGAC 2 cut(s) 222, 249
Bbv12I GWGCWC 2 cut(s) 228, 633
BbvI GCAGC 3 cut(s) 327, 617, 817
BccI CCATC 1 cut(s) 104
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 2 cut(s) 707, 801
BfmI CTRYAG 1 cut(s) 762
BfoI RGCGCY 1 cut(s) 19
BisI GCNGC 5 cut(s) 316, 363, 392, 606, 831
BlsI GCNGC 5 cut(s) 317, 364, 393, 607, 832
BmcAI AGTACT 1 cut(s) 537
Bme1390I CCNGG 2 cut(s) 707, 801
BmgT120I GGNCC 2 cut(s) 389, 588
BmiI GGNNCC 4 cut(s) 17, 377, 789, 798
BmrFI CCNGG 2 cut(s) 707, 801
BmsI GCATC 3 cut(s) 302, 592, 631
BpiI GAAGAC 2 cut(s) 222, 249
BpmI CTGGAG 2 cut(s) 130, 324
Bpu14I TTCGAA 1 cut(s) 323
BpuEI CTTGAG 1 cut(s) 547
BsaHI GRCGYC 1 cut(s) 16
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 372
Bse118I RCCGGY 2 cut(s) 365, 430
BseBI CCWGG 2 cut(s) 707, 801
BseLI CCNNNNNNNGG 2 cut(s) 200, 372
BseRI GAGGAG 4 cut(s) 251, 359, 641, 685
BseXI GCAGC 3 cut(s) 327, 617, 817
BseYI CCCAGC 2 cut(s) 82, 556
Bsh1236I CGCG 1 cut(s) 440
BshFI GGCC 3 cut(s) 362, 391, 589
BshNI GGYRCC 3 cut(s) 15, 375, 796
BsiHKAI GWGCWC 2 cut(s) 228, 633
BsiSI CCGG 2 cut(s) 366, 431
BslFI GGGAC 2 cut(s) 299, 497
BslI CCNNNNNNNGG 2 cut(s) 200, 372
BsmFI GGGAC 2 cut(s) 299, 497
BsnI GGCC 3 cut(s) 362, 391, 589
Bsp119I TTCGAA 1 cut(s) 323
Bsp1286I GDGCHC 3 cut(s) 228, 563, 633
Bsp143I GATC 2 cut(s) 154, 406
BspACI CCGC 9 cut(s) 34, 45, 175, 318, 356, 363, 392, 440, 617
BspANI GGCC 3 cut(s) 362, 391, 589
BspFNI CGCG 1 cut(s) 440
BspLI GGNNCC 4 cut(s) 17, 377, 789, 798
BspPI GGATC 1 cut(s) 149
BspT104I TTCGAA 1 cut(s) 323
BspT107I GGYRCC 3 cut(s) 15, 375, 796
BsrFI RCCGGY 2 cut(s) 365, 430
BssAI RCCGGY 2 cut(s) 365, 430
BssMI GATC 2 cut(s) 154, 406
BssNAI GTATAC 1 cut(s) 336
BssNI GRCGYC 1 cut(s) 16
Bst1107I GTATAC 1 cut(s) 336
Bst2UI CCWGG 2 cut(s) 707, 801
Bst4CI ACNGT 3 cut(s) 230, 290, 686
Bst6I CTCTTC 2 cut(s) 56, 228
BstACI GRCGYC 1 cut(s) 16
BstAPI GCANNNNNTGC 2 cut(s) 614, 641
BstBI TTCGAA 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 367, 633
BstDEI CTNAG 2 cut(s) 717, 834
BstFNI CGCG 1 cut(s) 440
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 2 cut(s) 157, 409
BstMBI GATC 2 cut(s) 154, 406
BstMWI GCNNNNNNNGC 5 cut(s) 362, 427, 614, 641, 794
BstNI CCWGG 2 cut(s) 707, 801
BstSCI CCNGG 2 cut(s) 705, 799
BstSFI CTRYAG 1 cut(s) 762
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 3 cut(s) 327, 617, 817
BstV2I GAAGAC 2 cut(s) 222, 249
BstXI CCANNNNNNTGG 1 cut(s) 386
BstZ17I GTATAC 1 cut(s) 336
BsuRI GGCC 3 cut(s) 362, 391, 589
BtgZI GCGATG 1 cut(s) 686
BtsIMutI CAGTG 3 cut(s) 235, 295, 768
Cac8I GCNNGC 2 cut(s) 367, 633
CaiI CAGNNNCTG 1 cut(s) 761
CfoI GCGC 1 cut(s) 18
Cfr10I RCCGGY 2 cut(s) 365, 430
Cfr13I GGNCC 2 cut(s) 389, 588
CsiI ACCWGGT 1 cut(s) 799
Csp6I GTAC 2 cut(s) 376, 536
CviAII CATG 2 cut(s) 553, 614
CviQI GTAC 2 cut(s) 376, 536
DdeI CTNAG 2 cut(s) 717, 834
DinI GGCGCC 1 cut(s) 17
DpnI GATC 2 cut(s) 156, 408
DpnII GATC 2 cut(s) 154, 406
Eam1104I CTCTTC 2 cut(s) 56, 228
EarI CTCTTC 2 cut(s) 56, 228
EciI GGCGGA 2 cut(s) 34, 371
Eco24I GRGCYC 1 cut(s) 563
Eco57I CTGAAG 1 cut(s) 380
EcoRII CCWGG 2 cut(s) 705, 799
EcoT38I GRGCYC 1 cut(s) 563
EgeI GGCGCC 1 cut(s) 17
EheI GGCGCC 1 cut(s) 17
FaeI CATG 2 cut(s) 556, 617
FaiI YATR 6 cut(s) 336, 424, 426, 554, 593, 615
FaqI GGGAC 2 cut(s) 299, 497
FatI CATG 2 cut(s) 552, 613
FauI CCCGC 2 cut(s) 41, 182
FauNDI CATATG 1 cut(s) 424
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 5 cut(s) 316, 363, 392, 606, 831
FriOI GRGCYC 1 cut(s) 563
Fsp4HI GCNGC 5 cut(s) 316, 363, 392, 606, 831
GlaI GCGC 1 cut(s) 17
GluI GCNGC 5 cut(s) 316, 363, 392, 606, 831
GsaI CCCAGC 2 cut(s) 86, 560
GsuI CTGGAG 2 cut(s) 130, 324
HaeII RGCGCY 1 cut(s) 19
HaeIII GGCC 3 cut(s) 362, 391, 589
HapII CCGG 2 cut(s) 366, 431
HhaI GCGC 1 cut(s) 18
Hin1I GRCGYC 1 cut(s) 16
Hin1II CATG 2 cut(s) 556, 617
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HinfI GANTC 1 cut(s) 198
HpaII CCGG 2 cut(s) 366, 431
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 2 cut(s) 336, 458
Hpy188I TCNGA 4 cut(s) 30, 399, 406, 573
Hpy188III TCNNGA 1 cut(s) 341
Hpy8I GTNNAC 2 cut(s) 336, 458
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 3 cut(s) 230, 290, 686
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 4 cut(s) 315, 489, 605, 644
HpyF10VI GCNNNNNNNGC 5 cut(s) 362, 427, 614, 641, 794
HpyF3I CTNAG 2 cut(s) 717, 834
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 16
Hsp92II CATG 2 cut(s) 556, 617
HspAI GCGC 1 cut(s) 16
KasI GGCGCC 1 cut(s) 15
KpnI GGTACC 1 cut(s) 379
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Kzo9I GATC 2 cut(s) 154, 406
LmnI GCTCC 3 cut(s) 91, 628, 793
Lsp1109I GCAGC 3 cut(s) 327, 617, 817
LweI GCATC 3 cut(s) 302, 592, 631
MabI ACCWGGT 1 cut(s) 799
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 1 cut(s) 766
MalI GATC 2 cut(s) 156, 408
MboI GATC 2 cut(s) 154, 406
MboII GAAGA 6 cut(s) 43, 227, 245, 254, 287, 688
MhlI GDGCHC 3 cut(s) 228, 563, 633
MluCI AATT 7 cut(s) 127, 509, 579, 651, 679, 710, 744
Mly113I GGCGCC 1 cut(s) 16
MroNI GCCGGC 1 cut(s) 365
MseI TTAA 1 cut(s) 660
MspA1I CMGCKG 1 cut(s) 318
MspI CCGG 2 cut(s) 366, 431
MspR9I CCNGG 2 cut(s) 707, 801
MvaI CCWGG 2 cut(s) 707, 801
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 5 cut(s) 362, 427, 614, 641, 794
NaeI GCCGGC 1 cut(s) 367
NarI GGCGCC 1 cut(s) 16
NdeI CATATG 1 cut(s) 424
NdeII GATC 2 cut(s) 154, 406
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 2 cut(s) 556, 617
NlaIV GGNNCC 4 cut(s) 17, 377, 789, 798
NmuCI GTSAC 1 cut(s) 766
NspV TTCGAA 1 cut(s) 323
PcsI WCGNNNNNNNCGW 5 cut(s) 252, 255, 258, 264, 267
PdiI GCCGGC 1 cut(s) 367
PfeI GAWTC 1 cut(s) 198
PkrI GCNGC 5 cut(s) 317, 364, 393, 607, 832
PluTI GGCGCC 1 cut(s) 19
Psp6I CCWGG 2 cut(s) 705, 799
PspFI CCCAGC 2 cut(s) 82, 556
PspGI CCWGG 2 cut(s) 705, 799
PspN4I GGNNCC 4 cut(s) 17, 377, 789, 798
PspPI GGNCC 2 cut(s) 389, 588
PstNI CAGNNNCTG 1 cut(s) 761
RsaI GTAC 2 cut(s) 377, 537
RsaNI GTAC 2 cut(s) 376, 536
SaqAI TTAA 1 cut(s) 660
SatI GCNGC 5 cut(s) 316, 363, 392, 606, 831
Sau3AI GATC 2 cut(s) 154, 406
Sau96I GGNCC 2 cut(s) 389, 588
ScaI AGTACT 1 cut(s) 537
ScrFI CCNGG 2 cut(s) 707, 801
SduI GDGCHC 3 cut(s) 228, 563, 633
SexAI ACCWGGT 1 cut(s) 799
SfaNI GCATC 3 cut(s) 302, 592, 631
SfcI CTRYAG 1 cut(s) 762
SfoI GGCGCC 1 cut(s) 17
SfuI TTCGAA 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 562
SmoI CTYRAG 1 cut(s) 562
Sse9I AATT 7 cut(s) 127, 509, 579, 651, 679, 710, 744
SsiI CCGC 9 cut(s) 34, 45, 175, 318, 356, 363, 392, 440, 617
SspDI GGCGCC 1 cut(s) 15
StyD4I CCNGG 2 cut(s) 705, 799
TaaI ACNGT 3 cut(s) 230, 290, 686
TaiI ACGT 1 cut(s) 210
TaqI TCGA 3 cut(s) 188, 323, 688
TasI AATT 7 cut(s) 127, 509, 579, 651, 679, 710, 744
TatI WGTACW 1 cut(s) 535
TauI GCSGC 2 cut(s) 365, 394
TfiI GAWTC 1 cut(s) 198
Tru1I TTAA 1 cut(s) 660
Tru9I TTAA 1 cut(s) 660
TscAI CASTG 3 cut(s) 235, 295, 775
TseFI GTSAC 1 cut(s) 766
TseI GCWGC 3 cut(s) 315, 605, 830
Tsp45I GTSAC 1 cut(s) 766
TspDTI ATGAA 4 cut(s) 57, 228, 288, 602
TspGWI ACGGA 2 cut(s) 512, 827
TspRI CASTG 3 cut(s) 235, 295, 775
XapI RAATTY 1 cut(s) 509
XmiI GTMKAC 1 cut(s) 335
ZrmI AGTACT 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.