Rh5BG061500

50S ribosomal protein L21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006560
Physical Location & Seq
Reverse (-)
5067389 .. 5069895
2507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG061500.1

Sequence Viewer

Length: 789 bp
ATGGCGAATCGACGGTGCCTCCAACTCCTGACCCGCCGCGTCTCCGCCCTATTCTCAGCCAACCCACCATTCCACACCCTCTCTACCCATCAACCCCTCAAAGCCCTCATCTCCAACTCCCCACTCGTTTTCACCCCCCATTGGTCCGCCCACACCCGCCATTTCTCGTCGGACCGAAACGACGACGTAAATGAGGACAGTCAAGACGACGACGACGACGACGACGATGATGAAATTGGGTACAGTAGTGACGACGGGAGTTTATCGAAGGGTGGTGGGTCGAAAAGAGAGTACACGGCGGAGGAGATGGAGGCGGAGGCGGCGGGTATTGGGTACAAAGTGGTTGGGCCGCTTGAGAAGACTGATGATGGGGTTTTCAAGCCCTATGAACCCGTTTTCGCTGTCATTCAGATTGGTTCGCATCAGTTCAAAGTTAGTAACGGGGACTCCATTTTCACTGAAAGATTGAAATTTTGCGAGGTCAATGATAAGTTGATATTGAATAAGGTTCTCTTGGTGGGCACAAGTAGTCAGACAATGATCGGCAGGCCAATAGTTCCTGATGCGGCAGTTCATGCAGTTGTTGAAGAGCATGCATTAGATGCAAAGGTACTTATTTTTAAGAAAAAGAGGAGGAAGAATTACCGTCGGACCAAAGGTCATCGCCAAGAATTAACCAAGTTGAGGATTATTGATATTCAAGGGATTCAGAAACCAGAACCTGTAGTCACCGAAAAACCTTCAAGGGCACCTGGTAAGAAGCCAGAAAAGGTCGCAGTTGCTGCATAG

Protein Analysis

262

Amino Acids

29.14

Weight (kDa)

6.76

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L21p PF00829 133 - 233 6.3e-32 Ribosomal prokaryotic L21 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 15, 748
AccII CGCG 1 cut(s) 39
AcsI RAATTY 1 cut(s) 470
AfaI GTAC 4 cut(s) 242, 293, 335, 612
AfiI CCNNNNNNNGG 2 cut(s) 141, 684
AgsI TTSAA 7 cut(s) 379, 430, 469, 502, 587, 701, 744
AhdI GACNNNNNGTC 1 cut(s) 657
AjnI CCWGG 1 cut(s) 751
AjuI GAANNNNNNNTTGG 2 cut(s) 53, 85
Alw26I GTCTC 1 cut(s) 46
AlwNI CAGNNNCTG 2 cut(s) 722, 782
AoxI GGCC 2 cut(s) 347, 548
ApeKI GCWGC 1 cut(s) 782
ApoI RAATTY 1 cut(s) 470
AspS9I GGNCC 4 cut(s) 144, 172, 347, 651
AsuHPI GGTGA 2 cut(s) 124, 721
AvaII GGWCC 3 cut(s) 144, 172, 651
BaeGI GKGCMC 2 cut(s) 524, 751
BaeI ACNNNNGTAYC 2 cut(s) 232, 265
BanI GGYRCC 2 cut(s) 15, 748
BbsI GAAGAC 1 cut(s) 365
BbvI GCAGC 1 cut(s) 769
BccI CCATC 3 cut(s) 96, 301, 362
BceAI ACGGC 1 cut(s) 312
BciT130I CCWGG 1 cut(s) 753
BcoDI GTCTC 1 cut(s) 46
BfmI CTRYAG 1 cut(s) 723
BisI GCNGC 5 cut(s) 37, 321, 350, 567, 783
BlsI GCNGC 5 cut(s) 38, 322, 351, 568, 784
Bme1390I CCNGG 1 cut(s) 753
Bme18I GGWCC 3 cut(s) 144, 172, 651
BmeRI GACNNNNNGTC 1 cut(s) 657
BmgT120I GGNCC 4 cut(s) 144, 172, 347, 651
BmiI GGNNCC 2 cut(s) 17, 750
BmrFI CCNGG 1 cut(s) 753
BmsI GCATC 3 cut(s) 430, 553, 592
BpiI GAAGAC 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 374
BsaXI ACNNNNNCTCC 3 cut(s) 33, 431, 461
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 684
BseBI CCWGG 1 cut(s) 753
BseLI CCNNNNNNNGG 2 cut(s) 141, 684
BseMII CTCAG 1 cut(s) 69
BseRI GAGGAG 2 cut(s) 317, 646
BseSI GKGCMC 2 cut(s) 524, 751
BseXI GCAGC 1 cut(s) 769
Bsh1236I CGCG 1 cut(s) 39
BshFI GGCC 2 cut(s) 349, 550
BshNI GGYRCC 2 cut(s) 15, 748
BslFI GGGAC 1 cut(s) 458
BslI CCNNNNNNNGG 2 cut(s) 141, 684
BsmAI GTCTC 1 cut(s) 46
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 458
BsnI GGCC 2 cut(s) 349, 550
Bsp1286I GDGCHC 2 cut(s) 524, 751
Bsp143I GATC 1 cut(s) 540
BspANI GGCC 2 cut(s) 349, 550
BspCNI CTCAG 1 cut(s) 68
BspFNI CGCG 1 cut(s) 39
BspLI GGNNCC 2 cut(s) 17, 750
BspQI GCTCTTC 1 cut(s) 582
BspT107I GGYRCC 2 cut(s) 15, 748
BssMI GATC 1 cut(s) 540
Bst2UI CCWGG 1 cut(s) 753
Bst4CI ACNGT 4 cut(s) 15, 200, 245, 647
Bst6I CTCTTC 1 cut(s) 582
BstAPI GCANNNNNTGC 3 cut(s) 575, 602, 782
BstC8I GCNNGC 2 cut(s) 548, 594
BstDEI CTNAG 1 cut(s) 55
BstFNI CGCG 1 cut(s) 39
BstKTI GATC 1 cut(s) 543
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 1 cut(s) 540
BstMWI GCNNNNNNNGC 4 cut(s) 320, 575, 602, 782
BstNI CCWGG 1 cut(s) 753
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 1 cut(s) 751
BstSFI CTRYAG 1 cut(s) 723
BstSLI GKGCMC 2 cut(s) 524, 751
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 769
BstV2I GAAGAC 1 cut(s) 365
BsuRI GGCC 2 cut(s) 349, 550
BtgZI GCGATG 1 cut(s) 647
BtsIMutI CAGTG 1 cut(s) 456
Cac8I GCNNGC 2 cut(s) 548, 594
CaiI CAGNNNCTG 2 cut(s) 722, 782
Cfr13I GGNCC 4 cut(s) 144, 172, 347, 651
CpoI CGGWCCG 1 cut(s) 172
CseI GACGC 1 cut(s) 28
CsiI ACCWGGT 1 cut(s) 751
Csp6I GTAC 4 cut(s) 241, 292, 334, 611
CspI CGGWCCG 1 cut(s) 172
CviAII CATG 2 cut(s) 575, 593
CviJI RGCY 6 cut(s) 59, 104, 349, 382, 550, 763
CviKI_1 RGCY 6 cut(s) 59, 104, 349, 382, 550, 763
CviQI GTAC 4 cut(s) 241, 292, 334, 611
DdeI CTNAG 1 cut(s) 55
DpnI GATC 1 cut(s) 542
DpnII GATC 1 cut(s) 540
DriI GACNNNNNGTC 1 cut(s) 657
Eam1104I CTCTTC 1 cut(s) 582
Eam1105I GACNNNNNGTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 582
EciI GGCGGA 4 cut(s) 34, 136, 314, 329
Eco47I GGWCC 3 cut(s) 144, 172, 651
EcoRII CCWGG 1 cut(s) 751
EcoT22I ATGCAT 1 cut(s) 598
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 2 cut(s) 578, 596
FaiI YATR 4 cut(s) 387, 576, 594, 787
FalI AAGNNNNNCTT 2 cut(s) 497, 529
FaqI GGGAC 1 cut(s) 458
FatI CATG 2 cut(s) 574, 592
FauI CCCGC 3 cut(s) 41, 164, 316
Fnu4HI GCNGC 5 cut(s) 37, 321, 350, 567, 783
Fsp4HI GCNGC 5 cut(s) 37, 321, 350, 567, 783
GluI GCNGC 5 cut(s) 37, 321, 350, 567, 783
HaeIII GGCC 2 cut(s) 349, 550
HgaI GACGC 1 cut(s) 28
Hin1II CATG 2 cut(s) 578, 596
HinfI GANTC 3 cut(s) 7, 446, 706
HphI GGTGA 2 cut(s) 124, 721
Hpy166II GTNNAC 1 cut(s) 294
Hpy188I TCNGA 5 cut(s) 172, 411, 534, 651, 711
Hpy188III TCNNGA 3 cut(s) 28, 203, 560
Hpy8I GTNNAC 1 cut(s) 294
HpyAV CCTTC 2 cut(s) 262, 750
HpyCH4III ACNGT 4 cut(s) 15, 200, 245, 647
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 4 cut(s) 578, 596, 605, 785
HpyF10VI GCNNNNNNNGC 4 cut(s) 320, 575, 602, 782
HpyF3I CTNAG 1 cut(s) 55
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 2 cut(s) 578, 596
Kzo9I GATC 1 cut(s) 540
LguI GCTCTTC 1 cut(s) 582
LpnPI CCDG 8 cut(s) 41, 532, 573, 729, 735, 738, 765, 777
Lsp1109I GCAGC 1 cut(s) 769
LweI GCATC 3 cut(s) 430, 553, 592
MabI ACCWGGT 1 cut(s) 751
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 3 cut(s) 248, 437, 727
MalI GATC 1 cut(s) 542
MboI GATC 1 cut(s) 540
MboII GAAGA 3 cut(s) 370, 599, 649
MhlI GDGCHC 2 cut(s) 524, 751
MluCI AATT 4 cut(s) 234, 470, 640, 671
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 4 cut(s) 46, 138, 150, 629
Mph1103I ATGCAT 1 cut(s) 598
MseI TTAA 2 cut(s) 621, 674
MspR9I CCNGG 1 cut(s) 753
MvaI CCWGG 1 cut(s) 753
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 4 cut(s) 320, 575, 602, 782
NdeII GATC 1 cut(s) 540
NlaIII CATG 2 cut(s) 578, 596
NlaIV GGNNCC 2 cut(s) 17, 750
NmuCI GTSAC 2 cut(s) 248, 727
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 1 cut(s) 596
PaeI GCATGC 1 cut(s) 596
PciSI GCTCTTC 1 cut(s) 582
PcsI WCGNNNNNNNCGW 4 cut(s) 213, 216, 219, 222
PfeI GAWTC 2 cut(s) 7, 706
PkrI GCNGC 5 cut(s) 38, 322, 351, 568, 784
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 751
PspGI CCWGG 1 cut(s) 751
PspN4I GGNNCC 2 cut(s) 17, 750
PspPI GGNCC 4 cut(s) 144, 172, 347, 651
PstNI CAGNNNCTG 2 cut(s) 722, 782
RsaI GTAC 4 cut(s) 242, 293, 335, 612
RsaNI GTAC 4 cut(s) 241, 292, 334, 611
Rsr2I CGGWCCG 1 cut(s) 172
RsrII CGGWCCG 1 cut(s) 172
SapI GCTCTTC 1 cut(s) 582
SaqAI TTAA 2 cut(s) 621, 674
SatI GCNGC 5 cut(s) 37, 321, 350, 567, 783
Sau3AI GATC 1 cut(s) 540
Sau96I GGNCC 4 cut(s) 144, 172, 347, 651
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 1 cut(s) 753
SduI GDGCHC 2 cut(s) 524, 751
SetI ASST 9 cut(s) 189, 483, 510, 612, 661, 724, 742, 754, 774
SexAI ACCWGGT 1 cut(s) 751
SfaNI GCATC 3 cut(s) 430, 553, 592
SfcI CTRYAG 1 cut(s) 723
SinI GGWCC 3 cut(s) 144, 172, 651
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SphI GCATGC 1 cut(s) 596
Sse9I AATT 4 cut(s) 234, 470, 640, 671
StyD4I CCNGG 1 cut(s) 751
TaaI ACNGT 4 cut(s) 15, 200, 245, 647
TaiI ACGT 1 cut(s) 189
TaqI TCGA 3 cut(s) 10, 266, 281
TaqII GACCGA 1 cut(s) 189
TasI AATT 4 cut(s) 234, 470, 640, 671
TatI WGTACW 1 cut(s) 291
TauI GCSGC 4 cut(s) 39, 323, 352, 569
TfiI GAWTC 2 cut(s) 7, 706
Tru1I TTAA 2 cut(s) 621, 674
Tru9I TTAA 2 cut(s) 621, 674
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 2 cut(s) 248, 727
TseI GCWGC 1 cut(s) 782
Tsp45I GTSAC 2 cut(s) 248, 727
TspDTI ATGAA 3 cut(s) 246, 402, 563
TspRI CASTG 1 cut(s) 463
VpaK11BI GGWCC 3 cut(s) 144, 172, 651
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 596
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.