Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006560

Physical Location & Seq

Genomic Coordinates Forward (+)

38227906 .. 38238989

Gene Structure 11084 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1294400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 831 bp

ATGGCGACCCGACGGGGCCTCCAAGCTCTGACCCGCCACGCCTCCGCCCTCTTCTCCATAAACCCTTCATTCCACTCCCTCCCAACCCCACCTATCCTCCCCATCTACCAACCCCTCAAATCCCTTACCTCCAATTTCTCTCTCATTTTCTCCAACCCCGATCCCACTCGCTCGCTTACCCGCCATTTCTCTTCGAAACCCCGAATCGGCGACGTAAATGAAGACAATGATCACAGTGACGATGATGAAGATGAAGATGAAGATGACGATGACAGTGAAGGAGTTGGGGACAGTGAGGACGAGAGTGCGTACCGCTGCAGCGGTTCGAAAAGAGAGTATACTCCAGAGGAGAAAGAGGTGGAGGCTGCCGCCATTGGGTACCAAGTGATTGGGCCGCTTCCGAGGTCTGATCGGGTTTTCAAGCCCTATGAGCCAGTTTTTGCCGTGGTTCAGGTTGGTTCACATCAGTTCAAGGTTAGCAATGGGGACTGCATTTTTACCGAAAAATTGAAATTCTGCGAAGTGAATGATAAGTTAGTATTGAACAAGGTTCTCATGCTGGGTACAAGCAGTCAGACAATAATCGGTAGGCCCATATTACCAGACGCAGCAGTTCATGCCGTTGTTGAGGAGCATGCATTAGATGCAAAGGTAATTATATTTAAGAAAAAGAGGAGGAAGAATTACCGTCGAACCAAAGGACATCGCCAGGAATTGACTAAGTTGAGGATAGTTGATGTTCAAGGGATTGAGAAACCAGAGCCTGTAGTCACTGAAAAGCCTTCAAAGGTACCTGGAAAGAAAAAGGAAAAGGTTGCAGCCGCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000741 GO:0003674 GO:0003676 GO:0003677 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0009553 GO:0009555 GO:0009559 GO:0009561 GO:0009566 GO:0009567 GO:0009987 GO:0010197 GO:0016043 GO:0019953 GO:0022414 GO:0030154 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0051704 GO:0071840 GO:0097159 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03010 map03010

Pfam Domains

Protein Families

Ribosomal_L21p

Protein Analysis

277

Amino Acids

30.79

Weight (kDa)

6.47

Isoelectric Point (pI)

38.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribosomal_L21p	PF00829	147 - 246	1.4e-31	Ribosomal prokaryotic L21 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006560)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30930
fragaria_vesca	FvH4_3g09190
malus_domestica	MD05G1312600.v1.1 MD10G1294400.v1.1
prunus_persica	Prupe.4G047500_v2.0.a1 Prupe.4G047500_v2.0.a1 Prupe.4G047500_v2.0.a1 Prupe.4G047500_v2.0.a1 Prupe.4G047500_v2.0.a1
pyrus_communis	pycom05g29050 pycom10g24610
rosa_chinensis	RchiOBHm_Chr5g0008411
rosa_laevigata	RLG00000031342 RLG00000031387 RLG00000031569 RLG00000035355
rosa_multiflora	Rmu_sc0004326.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068080
rosa_rugosa	Rorug04G0435600
rosa_samantha	Rh5AG064300 Rh5BG061500 Rh5CG071400 Rh5CG071700 Rh5DG060400 Rh5DG064100
rosa_wichuraiana	Rw5G005650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	378, 790
AccB1I	GGYRCC	2 cut(s)	378, 790
AccI	GTMKAC	1 cut(s)	338
AciI	CCGC	8 cut(s)	34, 45, 181, 313, 321, 369, 395, 822
AclWI	GGATC	1 cut(s)	155
AcsI	RAATTY	1 cut(s)	512
AfaI	GTAC	4 cut(s)	311, 380, 565, 792
AfiI	CCNNNNNNNGG	2 cut(s)	206, 375
AgsI	TTSAA	6 cut(s)	421, 472, 511, 544, 743, 786
AjnI	CCWGG	2 cut(s)	708, 793
AluBI	AGCT	1 cut(s)	26
AluI	AGCT	1 cut(s)	26
AlwI	GGATC	1 cut(s)	155
AlwNI	CAGNNNCTG	2 cut(s)	764, 824
AoxI	GGCC	3 cut(s)	16, 392, 590
ApeKI	GCWGC	6 cut(s)	315, 318, 365, 608, 818, 824
ApoI	RAATTY	1 cut(s)	512
Asp718I	GGTACC	2 cut(s)	378, 790
AspS9I	GGNCC	3 cut(s)	16, 392, 591
AsuII	TTCGAA	2 cut(s)	194, 326
BanI	GGYRCC	2 cut(s)	378, 790
BbsI	GAAGAC	1 cut(s)	228
BbvI	GCAGC	5 cut(s)	302, 330, 352, 620, 811
BccI	CCATC	1 cut(s)	110
BceAI	ACGGC	2 cut(s)	428, 605
BciT130I	CCWGG	2 cut(s)	710, 795
BclI	TGATCA	1 cut(s)	229
BfmI	CTRYAG	2 cut(s)	316, 765
BisI	GCNGC	9 cut(s)	316, 319, 366, 369, 395, 609, 819, 822, 825
BlsI	GCNGC	9 cut(s)	317, 320, 367, 370, 396, 610, 820, 823, 826
Bme1390I	CCNGG	2 cut(s)	710, 795
BmgT120I	GGNCC	3 cut(s)	16, 392, 591
BmiI	GGNNCC	3 cut(s)	17, 380, 792
BmrFI	CCNGG	2 cut(s)	710, 795
BmsI	GCATC	1 cut(s)	634
BpiI	GAAGAC	1 cut(s)	228
BpmI	CTGGAG	1 cut(s)	327
Bpu14I	TTCGAA	2 cut(s)	194, 326
BsaJI	CCNNGG	2 cut(s)	401, 444
BsaXI	ACNNNNNCTCC	3 cut(s)	33, 81, 111
Bsc4I	CCNNNNNNNGG	2 cut(s)	206, 375
Bse1I	ACTGG	1 cut(s)	434
Bse3DI	GCAATG	1 cut(s)	487
BseBI	CCWGG	2 cut(s)	710, 795
BseDI	CCNNGG	2 cut(s)	401, 444
BseLI	CCNNNNNNNGG	2 cut(s)	206, 375
BseMI	GCAATG	1 cut(s)	487
BseNI	ACTGG	1 cut(s)	434
BseRI	GAGGAG	3 cut(s)	362, 644, 688
BseXI	GCAGC	5 cut(s)	302, 330, 352, 620, 811
BseYI	CCCAGC	1 cut(s)	559
BshFI	GGCC	3 cut(s)	18, 394, 592
BshNI	GGYRCC	2 cut(s)	378, 790
BslFI	GGGAC	2 cut(s)	302, 500
BslI	CCNNNNNNNGG	2 cut(s)	206, 375
BsmFI	GGGAC	2 cut(s)	302, 500
BsnI	GGCC	3 cut(s)	18, 394, 592
Bsp119I	TTCGAA	2 cut(s)	194, 326
Bsp143I	GATC	3 cut(s)	160, 229, 409
BspACI	CCGC	8 cut(s)	34, 45, 181, 313, 321, 369, 395, 822
BspANI	GGCC	3 cut(s)	18, 394, 592
BspLI	GGNNCC	3 cut(s)	17, 380, 792
BspMAI	CTGCAG	1 cut(s)	320
BspPI	GGATC	1 cut(s)	155
BspT104I	TTCGAA	2 cut(s)	194, 326
BspT107I	GGYRCC	2 cut(s)	378, 790
BsrDI	GCAATG	1 cut(s)	487
BsrI	ACTGG	1 cut(s)	434
BssECI	CCNNGG	2 cut(s)	401, 444
BssMI	GATC	3 cut(s)	160, 229, 409
BssNAI	GTATAC	1 cut(s)	339
Bst1107I	GTATAC	1 cut(s)	339
Bst2UI	CCWGG	2 cut(s)	710, 795
Bst4CI	ACNGT	4 cut(s)	236, 275, 293, 689
Bst6I	CTCTTC	2 cut(s)	56, 196
BstAPI	GCANNNNNTGC	3 cut(s)	617, 644, 824
BstBI	TTCGAA	2 cut(s)	194, 326
BstC8I	GCNNGC	2 cut(s)	173, 636
BstDEI	CTNAG	2 cut(s)	720, 828
BstDSI	CCRYGG	1 cut(s)	444
BstKTI	GATC	3 cut(s)	163, 232, 412
BstMBI	GATC	3 cut(s)	160, 229, 409
BstMWI	GCNNNNNNNGC	4 cut(s)	430, 617, 644, 824
BstNI	CCWGG	2 cut(s)	710, 795
BstNSI	RCATGY	1 cut(s)	638
BstSCI	CCNGG	2 cut(s)	708, 793
BstSFI	CTRYAG	2 cut(s)	316, 765
BstV1I	GCAGC	5 cut(s)	302, 330, 352, 620, 811
BstV2I	GAAGAC	1 cut(s)	228
BstXI	CCANNNNNNTGG	1 cut(s)	389
BstZ17I	GTATAC	1 cut(s)	339
BsuRI	GGCC	3 cut(s)	18, 394, 592
BtgI	CCRYGG	1 cut(s)	444
BtgZI	GCGATG	1 cut(s)	689
BtsIMutI	CAGTG	4 cut(s)	241, 280, 298, 771
Cac8I	GCNNGC	2 cut(s)	173, 636
CaiI	CAGNNNCTG	2 cut(s)	764, 824
Cfr13I	GGNCC	3 cut(s)	16, 392, 591
CseI	GACGC	1 cut(s)	614
Csp6I	GTAC	4 cut(s)	310, 379, 564, 791
CviAII	CATG	3 cut(s)	556, 617, 635
CviQI	GTAC	4 cut(s)	310, 379, 564, 791
DdeI	CTNAG	2 cut(s)	720, 828
DpnI	GATC	3 cut(s)	162, 231, 411
DpnII	GATC	3 cut(s)	160, 229, 409
Eam1104I	CTCTTC	2 cut(s)	56, 196
EarI	CTCTTC	2 cut(s)	56, 196
EciI	GGCGGA	1 cut(s)	34
EcoO109I	RGGNCCY	1 cut(s)	16
EcoRII	CCWGG	2 cut(s)	708, 793
EcoT22I	ATGCAT	1 cut(s)	640
FaeI	CATG	3 cut(s)	559, 620, 638
FaiI	YATR	8 cut(s)	59, 339, 429, 557, 596, 618, 636, 659
FaqI	GGGAC	2 cut(s)	302, 500
FatI	CATG	3 cut(s)	555, 616, 634
FauI	CCCGC	2 cut(s)	41, 188
FbaI	TGATCA	1 cut(s)	229
FblI	GTMKAC	1 cut(s)	338
Fnu4HI	GCNGC	9 cut(s)	316, 319, 366, 369, 395, 609, 819, 822, 825
Fsp4HI	GCNGC	9 cut(s)	316, 319, 366, 369, 395, 609, 819, 822, 825
GluI	GCNGC	9 cut(s)	316, 319, 366, 369, 395, 609, 819, 822, 825
GsaI	CCCAGC	1 cut(s)	563
GsuI	CTGGAG	1 cut(s)	327
HaeIII	GGCC	3 cut(s)	18, 394, 592
HgaI	GACGC	1 cut(s)	614
Hin1II	CATG	3 cut(s)	559, 620, 638
HinfI	GANTC	1 cut(s)	204
Hpy166II	GTNNAC	2 cut(s)	339, 461
Hpy188I	TCNGA	4 cut(s)	30, 402, 409, 576
Hpy188III	TCNNGA	1 cut(s)	344
Hpy8I	GTNNAC	2 cut(s)	339, 461
Hpy99I	CGWCG	3 cut(s)	15, 215, 693
HpyAV	CCTTC	3 cut(s)	75, 272, 792
HpyCH4III	ACNGT	4 cut(s)	236, 275, 293, 689
HpyCH4IV	ACGT	1 cut(s)	213
HpyCH4V	TGCA	5 cut(s)	318, 492, 638, 647, 818
HpyF10VI	GCNNNNNNNGC	4 cut(s)	430, 617, 644, 824
HpyF3I	CTNAG	2 cut(s)	720, 828
HpySE526I	ACGT	1 cut(s)	213
Hsp92II	CATG	3 cut(s)	559, 620, 638
KpnI	GGTACC	2 cut(s)	382, 794
Ksp22I	TGATCA	1 cut(s)	229
Kzo9I	GATC	3 cut(s)	160, 229, 409
LmnI	GCTCC	1 cut(s)	631
Lsp1109I	GCAGC	5 cut(s)	302, 330, 352, 620, 811
LweI	GCATC	1 cut(s)	634
MaeII	ACGT	1 cut(s)	213
MaeIII	GTNAC	2 cut(s)	236, 769
MalI	GATC	3 cut(s)	162, 231, 411
MboI	GATC	3 cut(s)	160, 229, 409
MboII	GAAGA	7 cut(s)	43, 183, 233, 260, 266, 272, 691
MluCI	AATT	6 cut(s)	133, 506, 512, 654, 682, 713
MmeI	TCCRAC	1 cut(s)	177
Mph1103I	ATGCAT	1 cut(s)	640
MseI	TTAA	1 cut(s)	663
MspA1I	CMGCKG	3 cut(s)	315, 321, 824
MspR9I	CCNGG	2 cut(s)	710, 795
MvaI	CCWGG	2 cut(s)	710, 795
MwoI	GCNNNNNNNGC	4 cut(s)	430, 617, 644, 824
NdeII	GATC	3 cut(s)	160, 229, 409
NlaIII	CATG	3 cut(s)	559, 620, 638
NlaIV	GGNNCC	3 cut(s)	17, 380, 792
NmuCI	GTSAC	2 cut(s)	236, 769
NsiI	ATGCAT	1 cut(s)	640
NspI	RCATGY	1 cut(s)	638
NspV	TTCGAA	2 cut(s)	194, 326
PaeI	GCATGC	1 cut(s)	638
PfeI	GAWTC	1 cut(s)	204
PkrI	GCNGC	9 cut(s)	317, 320, 367, 370, 396, 610, 820, 823, 826
Psp6I	CCWGG	2 cut(s)	708, 793
PspFI	CCCAGC	1 cut(s)	559
PspGI	CCWGG	2 cut(s)	708, 793
PspN4I	GGNNCC	3 cut(s)	17, 380, 792
PspPI	GGNCC	3 cut(s)	16, 392, 591
PstI	CTGCAG	1 cut(s)	320
PstNI	CAGNNNCTG	2 cut(s)	764, 824
RsaI	GTAC	4 cut(s)	311, 380, 565, 792
RsaNI	GTAC	4 cut(s)	310, 379, 564, 791
SaqAI	TTAA	1 cut(s)	663
SatI	GCNGC	9 cut(s)	316, 319, 366, 369, 395, 609, 819, 822, 825
Sau3AI	GATC	3 cut(s)	160, 229, 409
Sau96I	GGNCC	3 cut(s)	16, 392, 591
ScrFI	CCNGG	2 cut(s)	710, 795
SfaNI	GCATC	1 cut(s)	634
SfcI	CTRYAG	2 cut(s)	316, 765
SfuI	TTCGAA	2 cut(s)	194, 326
SphI	GCATGC	1 cut(s)	638
Sse9I	AATT	6 cut(s)	133, 506, 512, 654, 682, 713
SsiI	CCGC	8 cut(s)	34, 45, 181, 313, 321, 369, 395, 822
StyD4I	CCNGG	2 cut(s)	708, 793
TaaI	ACNGT	4 cut(s)	236, 275, 293, 689
TaiI	ACGT	1 cut(s)	216
TaqI	TCGA	3 cut(s)	194, 326, 691
TasI	AATT	6 cut(s)	133, 506, 512, 654, 682, 713
TauI	GCSGC	3 cut(s)	371, 397, 824
TfiI	GAWTC	1 cut(s)	204
Tru1I	TTAA	1 cut(s)	663
Tru9I	TTAA	1 cut(s)	663
TscAI	CASTG	4 cut(s)	241, 280, 298, 778
TseFI	GTSAC	2 cut(s)	236, 769
TseI	GCWGC	6 cut(s)	315, 318, 365, 608, 818, 824
Tsp45I	GTSAC	2 cut(s)	236, 769
TspDTI	ATGAA	6 cut(s)	57, 234, 261, 267, 273, 605
TspRI	CASTG	4 cut(s)	241, 280, 298, 778
XapI	RAATTY	1 cut(s)	512
XceI	RCATGY	1 cut(s)	638
XmiI	GTMKAC	1 cut(s)	338
Zsp2I	ATGCAT	1 cut(s)	640

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.