MD05G1326100.v1.1
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001349
Physical Location & Seq
Forward (+)
45229747 .. 45233118
3372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1326100.v1.1.491

Sequence Viewer

Length: 1560 bp
ATGCCTGCTGTTGGTATCCCGGCCGGCGGGAACGGCAAGGCCTACGTCGGAAATCTCACTCCTTACGTTCTTGTCACCTGTGTCGTTGCCGCCATGGGTGGTCTGATTTTTGGCTACGATATCGGCATTTCCGGTGGGGTAACGTCCATGGACTCGTTCCTGAAGAAGTTCTTTCCGTCGGTGTACCACAAGAAGGAGAAGGACAAGACGACGAACCAGTACTGTCAGTATGATAGTCAAACGTTGACCATGTTCACATCGTCGCTGTACTTGGCTGCTCTCATATCCTCGATTGTGGCCGCCACCGTGACGCGTAAGTTTGGCCGGAAACTGTCCATGTTGTTCGGCGGTCTTCTGTTCTGCGCCGGTGCTATCATCAACGGCGCTGCCAAAGCTGTCTGGATGTTGATTGTCGGCCGTATGTTGCTCGGTTTCGGCATCGGATTCTCCAATCAGTCAGTGCCACTCTACCTGTCTGAGATGGCGCCCTACAGATTCAGGGGAGCTCTCAACATTGGCTTCCAGCTATCAATCACAGTTGGTATCCTTGTGGCCAATGTGCTGAACTACTTCTTTGCCAAGATAAAGGGTGGCTGGGGATGGAGGTTGAGCTTGGGCGGCGCGATGGTCCCCGCCCTTATCATCACAATCGGCTCACTTGTCCTGCCGGACACTCCCAACTCCATGATTGAGCGCGGTCAACACGACGAAGCCAAGGCCAAGCTCCAACGCATTCGCGGCGTTGATGATGTTAGGGAGGAGTTCAGTGACCTGGTAGCAGCTAGTGAAGCGGCGAACAAAATCGAGGACCCGTGGAGAAACTTGTTGAGGAAGAAGTACAGACCTCACCTCTGCATGGCCATCCTCATTCCCTTCTTTCAGCAACTCACTGGCATCAATGTGATCATGTTCTACGCGCCGGTTTTATTCAACACAATCGGGTTTGGAGCTGATGCCTCGCTCATGTCGGCTGTGATCACCGGCAGTGTTAATGTAATTGCAACTATGGTCTCAATCTATGGTGTTGACAAGTGGGGAAGAAGGTTCCTTTTCCTTGAGGGTGGAGCTCAAATGTTGATTTGCCAGGCAGTTGTGACAGCTTGTATTGCAGCAAAGTTTGGAGTGGATGGGAACCCTGGAGAATTGCCAAAGTGGTATGCAATTGTGGTGGTGTTGTTCATCTGCACATATGTTGCTGGATTCGCGTGGTCGTGGGGTCCCCTCGGATGGCTAGTCCCCAGTGAAATCTTCCCGTTGGAAATCCGATCAGCCGCACAGAGCGTCAACGTTTCAGTCAACATGATCTTCACCTTCATCGTGGCTCAAATCTTCTTGACAATGCTCTGCCACTTGAAGTTCGGGCTCTTCCTTTTCTTTGCATTCTTTGTGTTTGTGATGTCAATCTTCGTCTACTACTTCTTGCCGGAGACAAAGGGGATTCCGATCGAAGAGATGGGACAAGTATGGAGGACACATTGGTACTGGAAGAGATTCGTCAGTGAGGAAGCTGGAGGAGGTTATGAGATGGGCAAGGCAGCGCAACCTGTCAAAAATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

520

Amino Acids

57.05

Weight (kDa)

9.2

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 27 - 489 4e-138 Sugar (and other) transporter
MFS_1 PF07690 77 - 439 1.1e-25 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 484
AccI GTMKAC 1 cut(s) 1410
AccII CGCG 6 cut(s) 313, 623, 696, 738, 917, 1205
AclI AACGTT 2 cut(s) 242, 1287
AcoI YGGCCR 6 cut(s) 21, 297, 322, 415, 552, 858
AcuI CTGAAG 1 cut(s) 182
AcyI GRCGYC 1 cut(s) 485
AfaI GTAC 5 cut(s) 185, 221, 269, 839, 1481
AfiI CCNNNNNNNGG 5 cut(s) 11, 26, 193, 856, 1227
AflIII ACRYGT 1 cut(s) 311
AgsI TTSAA 2 cut(s) 931, 1354
AjnI CCWGG 3 cut(s) 771, 1083, 1135
Alw21I GWGCWC 2 cut(s) 508, 1069
Alw26I GTCTC 2 cut(s) 1015, 1421
AoxI GGCC 8 cut(s) 21, 39, 297, 322, 415, 552, 717, 858
ApeKI GCWGC 5 cut(s) 275, 386, 779, 1109, 1535
Asp700I GAANNNNTTC 3 cut(s) 167, 569, 1490
AspLEI GCGC 7 cut(s) 365, 386, 487, 623, 696, 919, 1540
AspS9I GGNCC 3 cut(s) 628, 808, 1217
AsuC2I CCSGG 1 cut(s) 20
AsuHPI GGTGA 4 cut(s) 67, 839, 970, 1300
AvaII GGWCC 3 cut(s) 628, 808, 1217
BalI TGGCCA 2 cut(s) 554, 860
BanI GGYRCC 1 cut(s) 484
BanII GRGCYC 3 cut(s) 508, 1069, 1365
BbsI GAAGAC 1 cut(s) 344
Bbv12I GWGCWC 2 cut(s) 508, 1069
BbvI GCAGC 5 cut(s) 262, 373, 791, 1121, 1547
BccI CCATC 8 cut(s) 475, 594, 619, 869, 1121, 1221, 1447, 1519
BceAI ACGGC 3 cut(s) 49, 397, 402
BciT130I CCWGG 3 cut(s) 773, 1085, 1137
BciVI GTATCC 2 cut(s) 26, 554
BclI TGATCA 2 cut(s) 903, 975
BcnI CCSGG 1 cut(s) 20
BcoDI GTCTC 2 cut(s) 1015, 1421
BfaI CTAG 2 cut(s) 783, 1232
BfmI CTRYAG 1 cut(s) 490
BfoI RGCGCY 2 cut(s) 387, 488
BfuI GTATCC 2 cut(s) 26, 554
BmcAI AGTACT 1 cut(s) 221
Bme1390I CCNGG 4 cut(s) 20, 773, 1085, 1137
Bme18I GGWCC 3 cut(s) 628, 808, 1217
BmgT120I GGNCC 3 cut(s) 628, 808, 1217
BmiI GGNNCC 7 cut(s) 486, 630, 810, 1046, 1133, 1218, 1219
BmrFI CCNGG 4 cut(s) 20, 773, 1085, 1137
BmrI ACTGGG 1 cut(s) 1233
BmsI GCATC 3 cut(s) 447, 903, 943
BmuI ACTGGG 1 cut(s) 1233
BpiI GAAGAC 1 cut(s) 344
BpmI CTGGAG 2 cut(s) 1158, 1530
BpuEI CTTGAG 1 cut(s) 1076
BpuMI CCSGG 1 cut(s) 20
BsaBI GATNNNNATC 2 cut(s) 1400, 1442
BsaHI GRCGYC 1 cut(s) 485
BsaI GGTCTC 1 cut(s) 1015
BsaJI CCNNGG 6 cut(s) 93, 147, 714, 812, 1135, 1222
BsaWI WCCGGW 1 cut(s) 131
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 26, 193, 856, 1227
Bse118I RCCGGY 4 cut(s) 23, 365, 919, 980
Bse1I ACTGG 4 cut(s) 217, 895, 1239, 1487
Bse8I GATNNNNATC 2 cut(s) 1400, 1442
BseBI CCWGG 3 cut(s) 773, 1085, 1137
BseDI CCNNGG 6 cut(s) 93, 147, 714, 812, 1135, 1222
BseGI GGATG 5 cut(s) 408, 605, 861, 1132, 1232
BseJI GATNNNNATC 2 cut(s) 1400, 1442
BseLI CCNNNNNNNGG 5 cut(s) 11, 26, 193, 856, 1227
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 4 cut(s) 217, 895, 1239, 1487
BseRI GAGGAG 2 cut(s) 773, 1527
BseX3I CGGCCG 2 cut(s) 21, 415
BseXI GCAGC 5 cut(s) 262, 373, 791, 1121, 1547
BseYI CCCAGC 1 cut(s) 594
BsgI GTGCAG 1 cut(s) 1168
Bsh1236I CGCG 6 cut(s) 313, 623, 696, 738, 917, 1205
Bsh1285I CGRYCG 3 cut(s) 24, 418, 1446
BshFI GGCC 8 cut(s) 23, 41, 299, 324, 417, 554, 719, 860
BshNI GGYRCC 1 cut(s) 484
BsiEI CGRYCG 3 cut(s) 24, 418, 1446
BsiHKAI GWGCWC 2 cut(s) 508, 1069
BsiSI CCGG 9 cut(s) 20, 24, 132, 325, 366, 668, 920, 981, 1424
BslFI GGGAC 4 cut(s) 614, 1203, 1220, 1470
BslI CCNNNNNNNGG 5 cut(s) 11, 26, 193, 856, 1227
BsmAI GTCTC 2 cut(s) 1015, 1421
BsmFI GGGAC 4 cut(s) 614, 1203, 1220, 1470
BsmI GAATGC 2 cut(s) 732, 1379
BsnI GGCC 8 cut(s) 23, 41, 299, 324, 417, 554, 719, 860
Bso31I GGTCTC 1 cut(s) 1015
Bsp1286I GDGCHC 3 cut(s) 508, 1069, 1365
Bsp143I GATC 5 cut(s) 903, 975, 1265, 1302, 1443
Bsp19I CCATGG 2 cut(s) 93, 147
BspANI GGCC 8 cut(s) 23, 41, 299, 324, 417, 554, 719, 860
BspCNI CTCAG 1 cut(s) 469
BspFNI CGCG 6 cut(s) 313, 623, 696, 738, 917, 1205
BspLI GGNNCC 7 cut(s) 486, 630, 810, 1046, 1133, 1218, 1219
BspQI GCTCTTC 1 cut(s) 1370
BspT107I GGYRCC 1 cut(s) 484
BspTNI GGTCTC 1 cut(s) 1015
BsrFI RCCGGY 4 cut(s) 23, 365, 919, 980
BsrI ACTGG 4 cut(s) 217, 895, 1239, 1487
BssAI RCCGGY 4 cut(s) 23, 365, 919, 980
BssECI CCNNGG 6 cut(s) 93, 147, 714, 812, 1135, 1222
BssMI GATC 5 cut(s) 903, 975, 1265, 1302, 1443
BssNI GRCGYC 1 cut(s) 485
BssT1I CCWWGG 3 cut(s) 93, 147, 714
Bst2UI CCWGG 3 cut(s) 773, 1085, 1137
Bst4CI ACNGT 4 cut(s) 224, 307, 333, 538
Bst6I CTCTTC 3 cut(s) 1370, 1443, 1481
BstACI GRCGYC 1 cut(s) 485
BstC8I GCNNGC 2 cut(s) 6, 25
BstDEI CTNAG 1 cut(s) 477
BstDSI CCRYGG 3 cut(s) 93, 147, 812
BstF5I GGATG 5 cut(s) 408, 605, 861, 1132, 1232
BstFNI CGCG 6 cut(s) 313, 623, 696, 738, 917, 1205
BstH2I RGCGCY 2 cut(s) 387, 488
BstHHI GCGC 7 cut(s) 365, 386, 487, 623, 696, 919, 1540
BstKTI GATC 5 cut(s) 906, 978, 1268, 1305, 1446
BstMAI GTCTC 2 cut(s) 1015, 1421
BstMBI GATC 5 cut(s) 903, 975, 1265, 1302, 1443
BstMCI CGRYCG 3 cut(s) 24, 418, 1446
BstMWI GCNNNNNNNGC 7 cut(s) 33, 392, 618, 738, 788, 1106, 1202
BstNI CCWGG 3 cut(s) 773, 1085, 1137
BstSCI CCNGG 4 cut(s) 18, 771, 1083, 1135
BstSFI CTRYAG 1 cut(s) 490
BstUI CGCG 6 cut(s) 313, 623, 696, 738, 917, 1205
BstV1I GCAGC 5 cut(s) 262, 373, 791, 1121, 1547
BstV2I GAAGAC 1 cut(s) 344
BstZI CGGCCG 2 cut(s) 21, 415
BsuI GTATCC 2 cut(s) 26, 554
BsuRI GGCC 8 cut(s) 23, 41, 299, 324, 417, 554, 719, 860
BtgI CCRYGG 3 cut(s) 93, 147, 812
BtgZI GCGATG 1 cut(s) 638
BtsCI GGATG 5 cut(s) 408, 605, 861, 1132, 1232
BtsI GCAGTG 1 cut(s) 991
BtsIMutI CAGTG 6 cut(s) 465, 772, 888, 991, 1246, 1504
Cac8I GCNNGC 2 cut(s) 6, 25
CfoI GCGC 7 cut(s) 365, 386, 487, 623, 696, 919, 1540
Cfr10I RCCGGY 4 cut(s) 23, 365, 919, 980
Cfr13I GGNCC 3 cut(s) 628, 808, 1217
CseI GACGC 2 cut(s) 319, 1270
CsiI ACCWGGT 1 cut(s) 771
Csp6I GTAC 5 cut(s) 184, 220, 268, 838, 1480
CspCI CAANNNNNGTGG 2 cut(s) 1149, 1184
CviAII CATG 9 cut(s) 94, 148, 250, 337, 685, 856, 907, 964, 1300
CviQI GTAC 5 cut(s) 184, 220, 268, 838, 1480
DdeI CTNAG 1 cut(s) 477
DinI GGCGCC 1 cut(s) 486
DpnI GATC 5 cut(s) 905, 977, 1267, 1304, 1445
DpnII GATC 5 cut(s) 903, 975, 1265, 1302, 1443
EaeI YGGCCR 6 cut(s) 21, 297, 322, 415, 552, 858
EagI CGGCCG 2 cut(s) 21, 415
Eam1104I CTCTTC 3 cut(s) 1370, 1443, 1481
EarI CTCTTC 3 cut(s) 1370, 1443, 1481
Ecl136II GAGCTC 2 cut(s) 506, 1067
EclXI CGGCCG 2 cut(s) 21, 415
Eco130I CCWWGG 3 cut(s) 93, 147, 714
Eco147I AGGCCT 1 cut(s) 41
Eco24I GRGCYC 3 cut(s) 508, 1069, 1365
Eco31I GGTCTC 1 cut(s) 1015
Eco32I GATATC 1 cut(s) 121
Eco47I GGWCC 3 cut(s) 628, 808, 1217
Eco52I CGGCCG 2 cut(s) 21, 415
Eco53kI GAGCTC 2 cut(s) 506, 1067
Eco57I CTGAAG 1 cut(s) 182
EcoICRI GAGCTC 2 cut(s) 506, 1067
EcoO109I RGGNCCY 2 cut(s) 808, 1217
EcoRII CCWGG 3 cut(s) 771, 1083, 1135
EcoRV GATATC 1 cut(s) 121
EcoT14I CCWWGG 3 cut(s) 93, 147, 714
EcoT38I GRGCYC 3 cut(s) 508, 1069, 1365
EgeI GGCGCC 1 cut(s) 486
EheI GGCGCC 1 cut(s) 486
ErhI CCWWGG 3 cut(s) 93, 147, 714
FaeI CATG 9 cut(s) 97, 151, 253, 340, 688, 859, 910, 967, 1303
FalI AAGNNNNNCTT 2 cut(s) 155, 187
FaqI GGGAC 4 cut(s) 614, 1203, 1220, 1470
FatI CATG 9 cut(s) 93, 147, 249, 336, 684, 855, 906, 963, 1299
FauI CCCGC 2 cut(s) 20, 640
FauNDI CATATG 1 cut(s) 1189
FbaI TGATCA 2 cut(s) 903, 975
FblI GTMKAC 1 cut(s) 1410
FokI GGATG 5 cut(s) 415, 612, 848, 1139, 1239
FriOI GRGCYC 3 cut(s) 508, 1069, 1365
FspBI CTAG 2 cut(s) 783, 1232
GlaI GCGC 7 cut(s) 364, 385, 486, 622, 695, 918, 1539
GsaI CCCAGC 1 cut(s) 598
GsuI CTGGAG 2 cut(s) 1158, 1530
HaeII RGCGCY 2 cut(s) 387, 488
HaeIII GGCC 8 cut(s) 23, 41, 299, 324, 417, 554, 719, 860
HapII CCGG 9 cut(s) 20, 24, 132, 325, 366, 668, 920, 981, 1424
HgaI GACGC 2 cut(s) 319, 1270
HhaI GCGC 7 cut(s) 365, 386, 487, 623, 696, 919, 1540
Hin1I GRCGYC 1 cut(s) 485
Hin1II CATG 9 cut(s) 97, 151, 253, 340, 688, 859, 910, 967, 1303
Hin6I GCGC 7 cut(s) 363, 384, 485, 621, 694, 917, 1538
HinP1I GCGC 7 cut(s) 363, 384, 485, 621, 694, 917, 1538
HincII GTYRAC 5 cut(s) 246, 701, 1027, 1285, 1297
HindII GTYRAC 5 cut(s) 246, 701, 1027, 1285, 1297
HinfI GANTC 6 cut(s) 152, 444, 495, 1200, 1438, 1491
HpaII CCGG 9 cut(s) 20, 24, 132, 325, 366, 668, 920, 981, 1424
HphI GGTGA 4 cut(s) 67, 839, 970, 1300
Hpy166II GTNNAC 8 cut(s) 184, 246, 255, 701, 1027, 1285, 1297, 1411
Hpy188I TCNGA 7 cut(s) 50, 105, 443, 478, 1226, 1265, 1443
Hpy188III TCNNGA 3 cut(s) 160, 400, 1333
Hpy8I GTNNAC 8 cut(s) 184, 246, 255, 701, 1027, 1285, 1297, 1411
Hpy99I CGWCG 5 cut(s) 50, 181, 214, 265, 710
HpyAV CCTTC 5 cut(s) 187, 193, 883, 1035, 1321
HpyCH4III ACNGT 4 cut(s) 224, 307, 333, 538
HpyCH4IV ACGT 5 cut(s) 45, 66, 143, 242, 1287
HpyCH4V TGCA 6 cut(s) 855, 1001, 1109, 1160, 1185, 1379
HpyF10VI GCNNNNNNNGC 7 cut(s) 33, 392, 618, 738, 788, 1106, 1202
HpyF3I CTNAG 1 cut(s) 477
HpySE526I ACGT 5 cut(s) 45, 66, 143, 242, 1287
Hsp92I GRCGYC 1 cut(s) 485
Hsp92II CATG 9 cut(s) 97, 151, 253, 340, 688, 859, 910, 967, 1303
HspAI GCGC 7 cut(s) 363, 384, 485, 621, 694, 917, 1538
KasI GGCGCC 1 cut(s) 484
KflI GGGWCCC 1 cut(s) 1217
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Ksp22I TGATCA 2 cut(s) 903, 975
Kzo9I GATC 5 cut(s) 903, 975, 1265, 1302, 1443
LguI GCTCTTC 1 cut(s) 1370
LmnI GCTCC 4 cut(s) 503, 729, 947, 1064
Lsp1109I GCAGC 5 cut(s) 262, 373, 791, 1121, 1547
LweI GCATC 3 cut(s) 447, 903, 943
MabI ACCWGGT 1 cut(s) 771
MaeI CTAG 2 cut(s) 783, 1232
MaeII ACGT 5 cut(s) 45, 66, 143, 242, 1287
MaeIII GTNAC 5 cut(s) 73, 139, 307, 767, 1093
MalI GATC 5 cut(s) 905, 977, 1267, 1304, 1445
MboI GATC 5 cut(s) 903, 975, 1265, 1302, 1443
MfeI CAATTG 1 cut(s) 1161
MhlI GDGCHC 3 cut(s) 508, 1069, 1365
MlsI TGGCCA 2 cut(s) 554, 860
MluCI AATT 3 cut(s) 996, 1142, 1161
MluI ACGCGT 1 cut(s) 311
MluNI TGGCCA 2 cut(s) 554, 860
Mly113I GGCGCC 1 cut(s) 485
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 3 cut(s) 28, 751, 1236
Mox20I TGGCCA 2 cut(s) 554, 860
MroNI GCCGGC 1 cut(s) 23
MroXI GAANNNNTTC 3 cut(s) 167, 569, 1490
MscI TGGCCA 2 cut(s) 554, 860
MseI TTAA 1 cut(s) 990
MslI CAYNNNNRTG 1 cut(s) 899
Msp20I TGGCCA 2 cut(s) 554, 860
MspI CCGG 9 cut(s) 20, 24, 132, 325, 366, 668, 920, 981, 1424
MspR9I CCNGG 4 cut(s) 20, 773, 1085, 1137
MunI CAATTG 1 cut(s) 1161
Mva1269I GAATGC 2 cut(s) 732, 1379
MvaI CCWGG 3 cut(s) 773, 1085, 1137
MvnI CGCG 6 cut(s) 313, 623, 696, 738, 917, 1205
MwoI GCNNNNNNNGC 7 cut(s) 33, 392, 618, 738, 788, 1106, 1202
NaeI GCCGGC 1 cut(s) 25
NarI GGCGCC 1 cut(s) 485
NciI CCSGG 1 cut(s) 20
NcoI CCATGG 2 cut(s) 93, 147
NdeI CATATG 1 cut(s) 1189
NdeII GATC 5 cut(s) 903, 975, 1265, 1302, 1443
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 9 cut(s) 97, 151, 253, 340, 688, 859, 910, 967, 1303
NlaIV GGNNCC 7 cut(s) 486, 630, 810, 1046, 1133, 1218, 1219
NmuCI GTSAC 4 cut(s) 73, 307, 767, 1093
PceI AGGCCT 1 cut(s) 41
PciSI GCTCTTC 1 cut(s) 1370
PctI GAATGC 2 cut(s) 732, 1379
PdiI GCCGGC 1 cut(s) 25
PdmI GAANNNNTTC 3 cut(s) 167, 569, 1490
PfeI GAWTC 5 cut(s) 444, 495, 1200, 1438, 1491
Ple19I CGATCG 1 cut(s) 1446
PleI GAGTC 1 cut(s) 146
PluTI GGCGCC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 146
PpuMI RGGWCCY 2 cut(s) 808, 1217
Psp124BI GAGCTC 2 cut(s) 508, 1069
Psp1406I AACGTT 2 cut(s) 242, 1287
Psp5II RGGWCCY 2 cut(s) 808, 1217
Psp6I CCWGG 3 cut(s) 771, 1083, 1135
PspFI CCCAGC 1 cut(s) 594
PspGI CCWGG 3 cut(s) 771, 1083, 1135
PspN4I GGNNCC 7 cut(s) 486, 630, 810, 1046, 1133, 1218, 1219
PspPI GGNCC 3 cut(s) 628, 808, 1217
PspPPI RGGWCCY 2 cut(s) 808, 1217
PvuI CGATCG 1 cut(s) 1446
RsaI GTAC 5 cut(s) 185, 221, 269, 839, 1481
RsaNI GTAC 5 cut(s) 184, 220, 268, 838, 1480
RseI CAYNNNNRTG 1 cut(s) 899
SacI GAGCTC 2 cut(s) 508, 1069
SapI GCTCTTC 1 cut(s) 1370
SaqAI TTAA 1 cut(s) 990
Sau3AI GATC 5 cut(s) 903, 975, 1265, 1302, 1443
Sau96I GGNCC 3 cut(s) 628, 808, 1217
ScaI AGTACT 1 cut(s) 221
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 4 cut(s) 20, 773, 1085, 1137
SduI GDGCHC 3 cut(s) 508, 1069, 1365
SexAI ACCWGGT 1 cut(s) 771
SfaNI GCATC 3 cut(s) 447, 903, 943
SfcI CTRYAG 1 cut(s) 490
SfoI GGCGCC 1 cut(s) 486
SgrAI CRCCGGYG 1 cut(s) 365
SinI GGWCC 3 cut(s) 628, 808, 1217
SmiMI CAYNNNNRTG 1 cut(s) 899
SmlI CTYRAG 1 cut(s) 1055
SmoI CTYRAG 1 cut(s) 1055
Sse9I AATT 3 cut(s) 996, 1142, 1161
SseBI AGGCCT 1 cut(s) 41
SspDI GGCGCC 1 cut(s) 484
SspMI CTAG 2 cut(s) 783, 1232
SstI GAGCTC 2 cut(s) 508, 1069
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 4 cut(s) 18, 771, 1083, 1135
StyI CCWWGG 3 cut(s) 93, 147, 714
TaaI ACNGT 4 cut(s) 224, 307, 333, 538
TaiI ACGT 5 cut(s) 48, 69, 146, 245, 1290
TaqI TCGA 3 cut(s) 290, 804, 1446
TasI AATT 3 cut(s) 996, 1142, 1161
TatI WGTACW 3 cut(s) 219, 267, 837
TauI GCSGC 6 cut(s) 92, 302, 621, 741, 794, 1274
TfiI GAWTC 5 cut(s) 444, 495, 1200, 1438, 1491
Tru1I TTAA 1 cut(s) 990
Tru9I TTAA 1 cut(s) 990
TscAI CASTG 6 cut(s) 465, 772, 895, 991, 1246, 1504
TseFI GTSAC 4 cut(s) 73, 307, 767, 1093
TseI GCWGC 5 cut(s) 275, 386, 779, 1109, 1535
Tsp45I GTSAC 4 cut(s) 73, 307, 767, 1093
TspDTI ATGAA 2 cut(s) 1168, 1303
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 6 cut(s) 465, 772, 895, 991, 1246, 1504
VpaK11BI GGWCC 3 cut(s) 628, 808, 1217
XmiI GTMKAC 1 cut(s) 1410
XmnI GAANNNNTTC 3 cut(s) 167, 569, 1490
XspI CTAG 2 cut(s) 783, 1232
ZrmI AGTACT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.