Rh5AG499300
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001349
Physical Location & Seq
Forward (+)
84606476 .. 84607226
751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG499300.1

Sequence Viewer

Length: 633 bp
ATGCCTGCCGTAGGTATTGCCAGTGGCGGCGGCAAGGAGTATGCCGGAAAACTCACCGGTTATGTTCTGGTAACATGCATCATCGCCGCCATGGGAGGGCTGATTTTCGGCTACGACATTGGAATCTCCGGTGGTGTAACGTCCATGGACTCGTTCCTGAAAAAGTTCTTTCCTTCTGTGTACCCCACCGTGACTAGGAAGTTTGGCCGGAAATTGTCCATGCTCTTCGGTGGTCTTCTCTTCTGCTCCGGCGCCATCATCAATGGCGCTGCCAAGGCTGTGTGGATGTTGATTTTTGGCCGGATACTCCTCGGTTTCGGTATCAGATTCTCCAATCAGTCTGTGCCACTCTACTTGTCTGAGATGGCGCCATCCAATTACAGAGGAGCTCTCAACATCTGTTTCCAGCTTTCTATCACAGTTGGTATCCTTGTCGCCAATTTGTTGAATTACTTCTTTGCCAAGATAGAGGGCGGTTGGGGGTGGCGTTTGAGTTTGGGTGGTGCAATGGTCCCTGCCCTTATCATCACCGTTGGCTCCCTAATATTACCAGACACACCAAACTCTATGATCGAGCGCGGCCAGCACGAGGAGGCCGGCCAAGACCCAACTCCGGAGGATTCGCGGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

210

Amino Acids

22.36

Weight (kDa)

8.93

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 35 - 195 9.2e-11 Major Facilitator Superfamily
Sugar_tr PF00083 62 - 199 5.8e-42 Sugar (and other) transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 251, 367
AccII CGCG 2 cut(s) 579, 625
AccIII TCCGGA 1 cut(s) 613
AciI CCGC 6 cut(s) 27, 30, 87, 474, 579, 625
AcoI YGGCCR 4 cut(s) 205, 298, 580, 598
AcyI GRCGYC 2 cut(s) 252, 368
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 5 cut(s) 11, 96, 195, 589, 613
AgeI ACCGGT 1 cut(s) 56
AgsI TTSAA 1 cut(s) 448
AluBI AGCT 2 cut(s) 389, 409
AluI AGCT 2 cut(s) 389, 409
Alw21I GWGCWC 1 cut(s) 391
Aor13HI TCCGGA 1 cut(s) 613
AoxI GGCC 5 cut(s) 205, 298, 580, 594, 598
ApeKI GCWGC 1 cut(s) 269
AsiGI ACCGGT 1 cut(s) 56
Asp700I GAANNNNTTC 2 cut(s) 164, 452
AspLEI GCGC 4 cut(s) 254, 269, 370, 579
AspS9I GGNCC 1 cut(s) 511
AsuHPI GGTGA 2 cut(s) 46, 520
AvaII GGWCC 1 cut(s) 511
BanI GGYRCC 2 cut(s) 251, 367
BanII GRGCYC 1 cut(s) 391
BauI CACGAG 1 cut(s) 587
BbsI GAAGAC 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 1 cut(s) 256
BccI CCATC 3 cut(s) 263, 358, 379
BciVI GTATCC 2 cut(s) 297, 437
BfaI CTAG 1 cut(s) 195
BfoI RGCGCY 3 cut(s) 255, 270, 371
BfuI GTATCC 2 cut(s) 297, 437
BisI GCNGC 5 cut(s) 28, 31, 87, 270, 580
BlsI GCNGC 5 cut(s) 29, 32, 88, 271, 581
Bme18I GGWCC 1 cut(s) 511
BmgT120I GGNCC 1 cut(s) 511
BmiI GGNNCC 4 cut(s) 253, 369, 513, 538
BmsI GCATC 1 cut(s) 87
BpiI GAAGAC 1 cut(s) 227
BplI GAGNNNNNCTC 2 cut(s) 375, 407
BsaHI GRCGYC 2 cut(s) 252, 368
BsaJI CCNNGG 4 cut(s) 90, 144, 273, 310
BsaWI WCCGGW 3 cut(s) 56, 128, 613
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 96, 195, 589, 613
Bse118I RCCGGY 2 cut(s) 56, 596
Bse1I ACTGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 513
BseAI TCCGGA 1 cut(s) 613
BseDI CCNNGG 4 cut(s) 90, 144, 273, 310
BseGI GGATG 2 cut(s) 291, 371
BseLI CCNNNNNNNGG 5 cut(s) 11, 96, 195, 589, 613
BseMI GCAATG 1 cut(s) 513
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 21
BseRI GAGGAG 3 cut(s) 299, 399, 605
BseXI GCAGC 1 cut(s) 256
Bsh1236I CGCG 2 cut(s) 579, 625
BshFI GGCC 5 cut(s) 207, 300, 582, 596, 600
BshNI GGYRCC 2 cut(s) 251, 367
BshTI ACCGGT 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 391
BsiSI CCGG 8 cut(s) 45, 57, 129, 208, 249, 301, 597, 614
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 5 cut(s) 11, 96, 195, 589, 613
BsmFI GGGAC 1 cut(s) 497
BsnI GGCC 5 cut(s) 207, 300, 582, 596, 600
Bsp1286I GDGCHC 1 cut(s) 391
Bsp13I TCCGGA 1 cut(s) 613
Bsp143I GATC 1 cut(s) 570
Bsp19I CCATGG 2 cut(s) 90, 144
BspACI CCGC 6 cut(s) 27, 30, 87, 474, 579, 625
BspANI GGCC 5 cut(s) 207, 300, 582, 596, 600
BspCNI CTCAG 1 cut(s) 352
BspEI TCCGGA 1 cut(s) 613
BspFNI CGCG 2 cut(s) 579, 625
BspLI GGNNCC 4 cut(s) 253, 369, 513, 538
BspQI GCTCTTC 1 cut(s) 230
BspT107I GGYRCC 2 cut(s) 251, 367
BsrDI GCAATG 1 cut(s) 513
BsrFI RCCGGY 2 cut(s) 56, 596
BsrI ACTGG 1 cut(s) 21
BssAI RCCGGY 2 cut(s) 56, 596
BssECI CCNNGG 4 cut(s) 90, 144, 273, 310
BssMI GATC 1 cut(s) 570
BssNI GRCGYC 2 cut(s) 252, 368
BssSI CACGAG 1 cut(s) 587
BssT1I CCWWGG 3 cut(s) 90, 144, 273
Bst2BI CACGAG 1 cut(s) 587
Bst4CI ACNGT 3 cut(s) 190, 421, 532
Bst6I CTCTTC 2 cut(s) 230, 245
BstACI GRCGYC 2 cut(s) 252, 368
BstC8I GCNNGC 3 cut(s) 6, 584, 598
BstDEI CTNAG 1 cut(s) 360
BstDSI CCRYGG 2 cut(s) 90, 144
BstENI CCTNNNNNAGG 1 cut(s) 9
BstF5I GGATG 2 cut(s) 291, 371
BstFNI CGCG 2 cut(s) 579, 625
BstH2I RGCGCY 3 cut(s) 255, 270, 371
BstHHI GCGC 4 cut(s) 254, 269, 370, 579
BstKTI GATC 1 cut(s) 573
BstMBI GATC 1 cut(s) 570
BstMWI GCNNNNNNNGC 2 cut(s) 275, 583
BstNSI RCATGY 1 cut(s) 78
BstUI CGCG 2 cut(s) 579, 625
BstV1I GCAGC 1 cut(s) 256
BstV2I GAAGAC 1 cut(s) 227
BsuI GTATCC 2 cut(s) 297, 437
BsuRI GGCC 5 cut(s) 207, 300, 582, 596, 600
BtgI CCRYGG 2 cut(s) 90, 144
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 2 cut(s) 291, 371
BtsIMutI CAGTG 1 cut(s) 28
Cac8I GCNNGC 3 cut(s) 6, 584, 598
CfoI GCGC 4 cut(s) 254, 269, 370, 579
Cfr10I RCCGGY 2 cut(s) 56, 596
Cfr13I GGNCC 1 cut(s) 511
Csp6I GTAC 1 cut(s) 181
CspAI ACCGGT 1 cut(s) 56
CspCI CAANNNNNGTGG 2 cut(s) 336, 371
CviAII CATG 4 cut(s) 75, 91, 145, 220
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 1 cut(s) 360
DinI GGCGCC 2 cut(s) 253, 369
DpnI GATC 1 cut(s) 572
DpnII GATC 1 cut(s) 570
EaeI YGGCCR 4 cut(s) 205, 298, 580, 598
Eam1104I CTCTTC 2 cut(s) 230, 245
EarI CTCTTC 2 cut(s) 230, 245
Ecl136II GAGCTC 1 cut(s) 389
Eco130I CCWWGG 3 cut(s) 90, 144, 273
Eco24I GRGCYC 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 511
Eco53kI GAGCTC 1 cut(s) 389
EcoICRI GAGCTC 1 cut(s) 389
EcoNI CCTNNNNNAGG 1 cut(s) 9
EcoT14I CCWWGG 3 cut(s) 90, 144, 273
EcoT22I ATGCAT 1 cut(s) 80
EcoT38I GRGCYC 1 cut(s) 391
EgeI GGCGCC 2 cut(s) 253, 369
EheI GGCGCC 2 cut(s) 253, 369
ErhI CCWWGG 3 cut(s) 90, 144, 273
FaeI CATG 4 cut(s) 78, 94, 148, 223
FaiI YATR 7 cut(s) 42, 63, 76, 92, 146, 221, 569
FaqI GGGAC 1 cut(s) 497
FatI CATG 4 cut(s) 74, 90, 144, 219
Fnu4HI GCNGC 5 cut(s) 28, 31, 87, 270, 580
FokI GGATG 2 cut(s) 298, 358
FriOI GRGCYC 1 cut(s) 391
FseI GGCCGGCC 1 cut(s) 600
Fsp4HI GCNGC 5 cut(s) 28, 31, 87, 270, 580
FspBI CTAG 1 cut(s) 195
GlaI GCGC 4 cut(s) 253, 268, 369, 578
GluI GCNGC 5 cut(s) 28, 31, 87, 270, 580
HaeII RGCGCY 3 cut(s) 255, 270, 371
HaeIII GGCC 5 cut(s) 207, 300, 582, 596, 600
HapII CCGG 8 cut(s) 45, 57, 129, 208, 249, 301, 597, 614
HhaI GCGC 4 cut(s) 254, 269, 370, 579
Hin1I GRCGYC 2 cut(s) 252, 368
Hin1II CATG 4 cut(s) 78, 94, 148, 223
Hin6I GCGC 4 cut(s) 252, 267, 368, 577
HinP1I GCGC 4 cut(s) 252, 267, 368, 577
HinfI GANTC 4 cut(s) 123, 149, 327, 620
HpaII CCGG 8 cut(s) 45, 57, 129, 208, 249, 301, 597, 614
HphI GGTGA 2 cut(s) 46, 520
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 2 cut(s) 326, 361
Hpy188III TCNNGA 2 cut(s) 157, 614
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 1 cut(s) 183
HpyCH4III ACNGT 3 cut(s) 190, 421, 532
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 2 cut(s) 78, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 583
HpyF3I CTNAG 1 cut(s) 360
HpySE526I ACGT 1 cut(s) 140
Hsp92I GRCGYC 2 cut(s) 252, 368
Hsp92II CATG 4 cut(s) 78, 94, 148, 223
HspAI GCGC 4 cut(s) 252, 267, 368, 577
KasI GGCGCC 2 cut(s) 251, 367
Kpn2I TCCGGA 1 cut(s) 613
KroI GCCGGC 1 cut(s) 596
KroNI GCCGGC 1 cut(s) 598
Kzo9I GATC 1 cut(s) 570
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 3 cut(s) 251, 386, 542
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 1 cut(s) 87
MaeI CTAG 1 cut(s) 195
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 3 cut(s) 70, 136, 190
MalI GATC 1 cut(s) 572
MboI GATC 1 cut(s) 570
MboII GAAGA 3 cut(s) 217, 227, 232
MhlI GDGCHC 1 cut(s) 391
MluCI AATT 4 cut(s) 212, 376, 439, 448
Mly113I GGCGCC 2 cut(s) 252, 368
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 7 cut(s) 89, 320, 377, 463, 583, 586, 610
Mph1103I ATGCAT 1 cut(s) 80
MroI TCCGGA 1 cut(s) 613
MroNI GCCGGC 1 cut(s) 596
MroXI GAANNNNTTC 2 cut(s) 164, 452
MspI CCGG 8 cut(s) 45, 57, 129, 208, 249, 301, 597, 614
MvnI CGCG 2 cut(s) 579, 625
MwoI GCNNNNNNNGC 2 cut(s) 275, 583
NaeI GCCGGC 1 cut(s) 598
NarI GGCGCC 2 cut(s) 252, 368
NcoI CCATGG 2 cut(s) 90, 144
NdeII GATC 1 cut(s) 570
NgoMIV GCCGGC 1 cut(s) 596
NlaIII CATG 4 cut(s) 78, 94, 148, 223
NlaIV GGNNCC 4 cut(s) 253, 369, 513, 538
NmuCI GTSAC 1 cut(s) 190
NsiI ATGCAT 1 cut(s) 80
NspI RCATGY 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 230
PdiI GCCGGC 1 cut(s) 598
PdmI GAANNNNTTC 2 cut(s) 164, 452
PfeI GAWTC 3 cut(s) 123, 327, 620
PinAI ACCGGT 1 cut(s) 56
PkrI GCNGC 5 cut(s) 29, 32, 88, 271, 581
PleI GAGTC 1 cut(s) 143
PluTI GGCGCC 2 cut(s) 255, 371
PpsI GAGTC 1 cut(s) 143
Psp124BI GAGCTC 1 cut(s) 391
PspN4I GGNNCC 4 cut(s) 253, 369, 513, 538
PspPI GGNCC 1 cut(s) 511
RigI GGCCGGCC 1 cut(s) 600
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
SacI GAGCTC 1 cut(s) 391
SapI GCTCTTC 1 cut(s) 230
SatI GCNGC 5 cut(s) 28, 31, 87, 270, 580
Sau3AI GATC 1 cut(s) 570
Sau96I GGNCC 1 cut(s) 511
SchI GAGTC 1 cut(s) 143
SduI GDGCHC 1 cut(s) 391
SetI ASST 4 cut(s) 16, 143, 391, 411
SfaNI GCATC 1 cut(s) 87
SfoI GGCGCC 2 cut(s) 253, 369
SinI GGWCC 1 cut(s) 511
Sse9I AATT 4 cut(s) 212, 376, 439, 448
SsiI CCGC 6 cut(s) 27, 30, 87, 474, 579, 625
SspDI GGCGCC 2 cut(s) 251, 367
SspI AATATT 1 cut(s) 546
SspMI CTAG 1 cut(s) 195
SstI GAGCTC 1 cut(s) 391
StyI CCWWGG 3 cut(s) 90, 144, 273
TaaI ACNGT 3 cut(s) 190, 421, 532
TaiI ACGT 1 cut(s) 143
TaqI TCGA 1 cut(s) 573
TasI AATT 4 cut(s) 212, 376, 439, 448
TauI GCSGC 4 cut(s) 30, 33, 89, 582
TfiI GAWTC 3 cut(s) 123, 327, 620
TscAI CASTG 1 cut(s) 28
TseFI GTSAC 1 cut(s) 190
TseI GCWGC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 190
TspRI CASTG 1 cut(s) 28
VpaK11BI GGWCC 1 cut(s) 511
XagI CCTNNNNNAGG 1 cut(s) 9
XceI RCATGY 1 cut(s) 78
XmnI GAANNNNTTC 2 cut(s) 164, 452
XspI CTAG 1 cut(s) 195
Zsp2I ATGCAT 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.