Rw5G046330
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001349
Physical Location & Seq
Forward (+)
80463570 .. 80465129
1560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G046330.1

Sequence Viewer

Length: 1560 bp
ATGCCTGCCGTAGGTATTGCCAGTGGCGGCGGCAAGGAGTATGCCGGAAAACTCACCGGTTATGTTCTGGTAACATGCATCATCGCCGCCATGGGAGGGCTGATTTTCGGCTACGACATTGGAATCTCCGGTGGCGTAACGTCCATGGACTCGTTCCTGAAAAAGTTCTTTCCTTCTGTGTACCGTAAGAAGGAGCTGGACAAGTCTACCAACCAGTACTGCCAGTATGACAGTCAGACGTTGACCATGTTCACGTCTTCCTTATACTTGGCTGCTCTCATGGCCTCAGTTGTGGCAGCCACCGTGACTCGGAAGTTTGGCCGGAAATTGTCCATGCTTTTCGGTGGTCTTCTCTTCTGCTCCGGCGCCATCATCAATGGCGCTGCCAAGGCTGTGTGGATGTTGATTTTTGGCCGGATACTCCTCGGTTTCGGTATCGGATTCTCCAATCAGTCTGTGCCACTCTACTTGTCTGAGATGGCGCCATCCAATTACAGAGGAGCTCTCAACATTGGTTTCCAGCTTTCTATCACAGTTGGTATCCTTGTCGCCAATTTGTTGAATTACTTCTTTGCCAAGATAGAGGGCGGTTGGGGATGGCGTTTGAGTTTGGGTGGTGCAATGGTCCCTGCCCTTATCATCACCGTTGGCTCCCTAATATTACCAGACACACCAAACTCTATGATCGAGCGCGGCCAGCACGAGGAGGCCAAGACCCAACTCCGGAGGATTCGCGGCGTTGATGATGTCAGCGACGAGTTGAATGACCTTGTTATGGCTAGTGATGAGTCCAAGAAGATAGAAGATCCATGGAGGAATCTGCTCCAGAGGAAGTACAGGCCCCAACTTTGCATGGCAATTCTGATTCCATTCTTTCAGCAGCTTACCGGTATCAATGTGATCATGTTCTATGCCCCTGTGTTGTTCAACACAATTGGTTTCGGAAGTGATGCTTCTCTCATATCGGCTGTGATCACCGGACTTGTTAATGTTGCTGCAACAATCGTTTCGATGTATGGTGTTGATAAGTGGGGAAGAAGGAAACTCTTCCTTGAGGGAGGAACTCAAATGTTGATATGCCAAATAGTTGTGGCGGCTTGCATTGGTGCAAAATTTGGATTGGATGGAAATGCCGGTAACTTGCCAAAGTGGTATGCAATTGTTGTAGTAGTTTTCATCTGCACATATGTAGCAGGGTTTGCTTGGTCATGGGGTCCTCTAGGGTGGCTAGTCCCTAGTGAAATATTCCCCTTGGAAATCAGGTCAGCTGCTCAGAGTGTCAATGTCTCAGTCAACATGATCTTCACCTTCATTGTGGCTCAAGTCTTCTTGACAATGTTATGCCACATGAAATTCGGGCTCTTCATCTTCTTCGCATTCTTTGTGTGTGTCATGTCCGTTTTCGTGTACTTTTTCTTGCCCGAGACAAAGGGGATCCCAATTGAAGAGATGAGCAGGGTTTGGAAACAGCATTGGTACTGGAAGAGATTTGTTACCGATGAAGATTACCCCAACGATGGTTATGAGATGAGCAAGCCAACAATTGTCAACATTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

519

Amino Acids

57.32

Weight (kDa)

8.8

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 26 - 488 4.7e-138 Sugar (and other) transporter
MFS_1 PF07690 76 - 438 1.4e-26 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 365, 481
AccI GTMKAC 1 cut(s) 206
AccII CGCG 2 cut(s) 693, 735
AccIII TCCGGA 1 cut(s) 723
AciI CCGC 7 cut(s) 27, 30, 87, 588, 693, 735, 1094
AclWI GGATC 3 cut(s) 800, 1429, 1442
AcoI YGGCCR 3 cut(s) 319, 412, 694
AcsI RAATTY 2 cut(s) 1112, 1352
AcyI GRCGYC 2 cut(s) 366, 482
AfaI GTAC 5 cut(s) 182, 218, 836, 1409, 1478
AfiI CCNNNNNNNGG 8 cut(s) 11, 96, 190, 309, 703, 723, 775, 1517
AgeI ACCGGT 2 cut(s) 56, 887
AgsI TTSAA 4 cut(s) 562, 763, 928, 1445
AjiI CACGTC 1 cut(s) 255
AluBI AGCT 5 cut(s) 196, 503, 523, 883, 1268
AluI AGCT 5 cut(s) 196, 503, 523, 883, 1268
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 2 cut(s) 1291, 1418
AlwI GGATC 3 cut(s) 800, 1429, 1442
Ama87I CYCGRG 1 cut(s) 1421
Aor13HI TCCGGA 1 cut(s) 723
AoxI GGCC 6 cut(s) 282, 319, 412, 694, 708, 839
ApeKI GCWGC 6 cut(s) 272, 296, 383, 880, 995, 1268
ApoI RAATTY 2 cut(s) 1112, 1352
AsiGI ACCGGT 2 cut(s) 56, 887
Asp700I GAANNNNTTC 3 cut(s) 164, 566, 1046
AspLEI GCGC 4 cut(s) 368, 383, 484, 693
AspS9I GGNCC 3 cut(s) 625, 840, 1214
AsuHPI GGTGA 4 cut(s) 46, 634, 967, 1297
AvaI CYCGRG 1 cut(s) 1421
AvaII GGWCC 2 cut(s) 625, 1214
BamHI GGATCC 1 cut(s) 1434
BanI GGYRCC 2 cut(s) 365, 481
BanII GRGCYC 2 cut(s) 505, 1362
BauI CACGAG 1 cut(s) 701
BbsI GAAGAC 3 cut(s) 249, 341, 1318
Bbv12I GWGCWC 1 cut(s) 505
BbvI GCAGC 6 cut(s) 259, 308, 370, 892, 982, 1255
BccI CCATC 6 cut(s) 377, 472, 493, 591, 1118, 1511
BciVI GTATCC 2 cut(s) 411, 551
BclI TGATCA 2 cut(s) 900, 972
BcoDI GTCTC 2 cut(s) 1291, 1418
BfaI CTAG 4 cut(s) 780, 1220, 1229, 1236
BfoI RGCGCY 3 cut(s) 369, 384, 485
BfuI GTATCC 2 cut(s) 411, 551
BmcAI AGTACT 1 cut(s) 218
Bme18I GGWCC 2 cut(s) 625, 1214
BmeT110I CYCGRG 1 cut(s) 1421
BmgBI CACGTC 1 cut(s) 255
BmgT120I GGNCC 3 cut(s) 625, 840, 1214
BmiI GGNNCC 7 cut(s) 367, 483, 627, 652, 842, 1215, 1436
BmsI GCATC 2 cut(s) 87, 940
BpiI GAAGAC 3 cut(s) 249, 341, 1318
BplI GAGNNNNNCTC 2 cut(s) 489, 521
BpmI CTGGAG 1 cut(s) 809
BpuEI CTTGAG 2 cut(s) 1073, 1305
BsaHI GRCGYC 2 cut(s) 366, 482
BsaJI CCNNGG 6 cut(s) 90, 144, 387, 424, 809, 1251
BsaWI WCCGGW 5 cut(s) 56, 128, 723, 887, 977
Bsc4I CCNNNNNNNGG 8 cut(s) 11, 96, 190, 309, 703, 723, 775, 1517
Bse118I RCCGGY 3 cut(s) 56, 887, 1133
Bse1I ACTGG 4 cut(s) 21, 214, 223, 1484
Bse3DI GCAATG 1 cut(s) 627
BseAI TCCGGA 1 cut(s) 723
BseDI CCNNGG 6 cut(s) 90, 144, 387, 424, 809, 1251
BseGI GGATG 4 cut(s) 405, 485, 602, 1129
BseLI CCNNNNNNNGG 8 cut(s) 11, 96, 190, 309, 703, 723, 775, 1517
BseMI GCAATG 1 cut(s) 627
BseMII CTCAG 4 cut(s) 300, 465, 1286, 1302
BseNI ACTGG 4 cut(s) 21, 214, 223, 1484
BseRI GAGGAG 3 cut(s) 413, 513, 719
BseXI GCAGC 6 cut(s) 259, 308, 370, 892, 982, 1255
BsgI GTGCAG 1 cut(s) 1165
Bsh1236I CGCG 2 cut(s) 693, 735
BshFI GGCC 6 cut(s) 284, 321, 414, 696, 710, 841
BshNI GGYRCC 2 cut(s) 365, 481
BshTI ACCGGT 2 cut(s) 56, 887
BsiHKAI GWGCWC 1 cut(s) 505
BsiHKCI CYCGRG 1 cut(s) 1421
BslFI GGGAC 2 cut(s) 611, 1217
BslI CCNNNNNNNGG 8 cut(s) 11, 96, 190, 309, 703, 723, 775, 1517
BsmAI GTCTC 2 cut(s) 1291, 1418
BsmFI GGGAC 2 cut(s) 611, 1217
BsmI GAATGC 1 cut(s) 1376
BsnI GGCC 6 cut(s) 284, 321, 414, 696, 710, 841
BsoBI CYCGRG 1 cut(s) 1421
Bsp1286I GDGCHC 2 cut(s) 505, 1362
Bsp13I TCCGGA 1 cut(s) 723
Bsp143I GATC 6 cut(s) 684, 805, 900, 972, 1299, 1434
Bsp19I CCATGG 3 cut(s) 90, 144, 809
BspACI CCGC 7 cut(s) 27, 30, 87, 588, 693, 735, 1094
BspANI GGCC 6 cut(s) 284, 321, 414, 696, 710, 841
BspCNI CTCAG 4 cut(s) 299, 466, 1285, 1301
BspEI TCCGGA 1 cut(s) 723
BspFNI CGCG 2 cut(s) 693, 735
BspLI GGNNCC 7 cut(s) 367, 483, 627, 652, 842, 1215, 1436
BspPI GGATC 3 cut(s) 800, 1429, 1442
BspQI GCTCTTC 1 cut(s) 1367
BspT107I GGYRCC 2 cut(s) 365, 481
BsrDI GCAATG 1 cut(s) 627
BsrFI RCCGGY 3 cut(s) 56, 887, 1133
BsrI ACTGG 4 cut(s) 21, 214, 223, 1484
BssAI RCCGGY 3 cut(s) 56, 887, 1133
BssECI CCNNGG 6 cut(s) 90, 144, 387, 424, 809, 1251
BssMI GATC 6 cut(s) 684, 805, 900, 972, 1299, 1434
BssNI GRCGYC 2 cut(s) 366, 482
BssSI CACGAG 1 cut(s) 701
BssT1I CCWWGG 5 cut(s) 90, 144, 387, 809, 1251
Bst2BI CACGAG 1 cut(s) 701
Bst4CI ACNGT 5 cut(s) 185, 233, 304, 535, 646
Bst6I CTCTTC 5 cut(s) 359, 1052, 1367, 1440, 1478
BstACI GRCGYC 2 cut(s) 366, 482
BstAPI GCANNNNNTGC 1 cut(s) 1199
BstC8I GCNNGC 4 cut(s) 6, 698, 1099, 1535
BstDEI CTNAG 4 cut(s) 286, 474, 1272, 1288
BstDSI CCRYGG 3 cut(s) 90, 144, 809
BstENI CCTNNNNNAGG 1 cut(s) 9
BstF5I GGATG 4 cut(s) 405, 485, 602, 1129
BstFNI CGCG 2 cut(s) 693, 735
BstH2I RGCGCY 3 cut(s) 369, 384, 485
BstHHI GCGC 4 cut(s) 368, 383, 484, 693
BstKTI GATC 6 cut(s) 687, 808, 903, 975, 1302, 1437
BstMAI GTCTC 2 cut(s) 1291, 1418
BstMBI GATC 6 cut(s) 684, 805, 900, 972, 1299, 1434
BstMWI GCNNNNNNNGC 4 cut(s) 281, 389, 697, 1199
BstNSI RCATGY 1 cut(s) 78
BstUI CGCG 2 cut(s) 693, 735
BstV1I GCAGC 6 cut(s) 259, 308, 370, 892, 982, 1255
BstV2I GAAGAC 3 cut(s) 249, 341, 1318
BstX2I RGATCY 2 cut(s) 805, 1434
BstYI RGATCY 2 cut(s) 805, 1434
BsuI GTATCC 2 cut(s) 411, 551
BsuRI GGCC 6 cut(s) 284, 321, 414, 696, 710, 841
BtgI CCRYGG 3 cut(s) 90, 144, 809
BtgZI GCGATG 1 cut(s) 67
BtrI CACGTC 1 cut(s) 255
BtsCI GGATG 4 cut(s) 405, 485, 602, 1129
BtsIMutI CAGTG 1 cut(s) 28
Cac8I GCNNGC 4 cut(s) 6, 698, 1099, 1535
CfoI GCGC 4 cut(s) 368, 383, 484, 693
Cfr10I RCCGGY 3 cut(s) 56, 887, 1133
Cfr13I GGNCC 3 cut(s) 625, 840, 1214
Csp6I GTAC 5 cut(s) 181, 217, 835, 1408, 1477
CspAI ACCGGT 2 cut(s) 56, 887
CspCI CAANNNNNGTGG 2 cut(s) 450, 485
CviQI GTAC 5 cut(s) 181, 217, 835, 1408, 1477
DdeI CTNAG 4 cut(s) 286, 474, 1272, 1288
DinI GGCGCC 2 cut(s) 367, 483
DpnI GATC 6 cut(s) 686, 807, 902, 974, 1301, 1436
DpnII GATC 6 cut(s) 684, 805, 900, 972, 1299, 1434
EaeI YGGCCR 3 cut(s) 319, 412, 694
Eam1104I CTCTTC 5 cut(s) 359, 1052, 1367, 1440, 1478
EarI CTCTTC 5 cut(s) 359, 1052, 1367, 1440, 1478
Ecl136II GAGCTC 1 cut(s) 503
Eco130I CCWWGG 5 cut(s) 90, 144, 387, 809, 1251
Eco24I GRGCYC 2 cut(s) 505, 1362
Eco47I GGWCC 2 cut(s) 625, 1214
Eco53kI GAGCTC 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 1421
EcoICRI GAGCTC 1 cut(s) 503
EcoNI CCTNNNNNAGG 1 cut(s) 9
EcoO109I RGGNCCY 2 cut(s) 840, 1214
EcoT14I CCWWGG 5 cut(s) 90, 144, 387, 809, 1251
EcoT22I ATGCAT 1 cut(s) 80
EcoT38I GRGCYC 2 cut(s) 505, 1362
EgeI GGCGCC 2 cut(s) 367, 483
EheI GGCGCC 2 cut(s) 367, 483
ErhI CCWWGG 5 cut(s) 90, 144, 387, 809, 1251
FalI AAGNNNNNCTT 2 cut(s) 937, 969
FaqI GGGAC 2 cut(s) 611, 1217
FauNDI CATATG 1 cut(s) 1186
FbaI TGATCA 2 cut(s) 900, 972
FblI GTMKAC 1 cut(s) 206
FokI GGATG 4 cut(s) 412, 472, 609, 1136
FriOI GRGCYC 2 cut(s) 505, 1362
FspBI CTAG 4 cut(s) 780, 1220, 1229, 1236
GlaI GCGC 4 cut(s) 367, 382, 483, 692
GsuI CTGGAG 1 cut(s) 809
HaeII RGCGCY 3 cut(s) 369, 384, 485
HaeIII GGCC 6 cut(s) 284, 321, 414, 696, 710, 841
HhaI GCGC 4 cut(s) 368, 383, 484, 693
Hin1I GRCGYC 2 cut(s) 366, 482
Hin6I GCGC 4 cut(s) 366, 381, 482, 691
HinP1I GCGC 4 cut(s) 366, 381, 482, 691
HincII GTYRAC 3 cut(s) 243, 1294, 1549
HindII GTYRAC 3 cut(s) 243, 1294, 1549
HinfI GANTC 8 cut(s) 123, 149, 307, 441, 730, 788, 817, 865
HphI GGTGA 4 cut(s) 46, 634, 967, 1297
Hpy166II GTNNAC 7 cut(s) 181, 207, 243, 252, 1294, 1408, 1549
Hpy188I TCNGA 7 cut(s) 237, 312, 440, 475, 864, 944, 1275
Hpy188III TCNNGA 4 cut(s) 157, 724, 826, 1330
Hpy8I GTNNAC 7 cut(s) 181, 207, 243, 252, 1294, 1408, 1549
Hpy99I CGWCG 1 cut(s) 758
HpyAV CCTTC 4 cut(s) 183, 184, 1032, 1318
HpyCH4III ACNGT 5 cut(s) 185, 233, 304, 535, 646
HpyCH4IV ACGT 3 cut(s) 140, 239, 254
HpyCH4V TGCA 8 cut(s) 78, 620, 852, 998, 1101, 1109, 1157, 1182
HpyF10VI GCNNNNNNNGC 4 cut(s) 281, 389, 697, 1199
HpyF3I CTNAG 4 cut(s) 286, 474, 1272, 1288
HpySE526I ACGT 3 cut(s) 140, 239, 254
Hsp92I GRCGYC 2 cut(s) 366, 482
HspAI GCGC 4 cut(s) 366, 381, 482, 691
KasI GGCGCC 2 cut(s) 365, 481
Kpn2I TCCGGA 1 cut(s) 723
Ksp22I TGATCA 2 cut(s) 900, 972
Kzo9I GATC 6 cut(s) 684, 805, 900, 972, 1299, 1434
LguI GCTCTTC 1 cut(s) 1367
LmnI GCTCC 5 cut(s) 193, 365, 500, 656, 828
Lsp1109I GCAGC 6 cut(s) 259, 308, 370, 892, 982, 1255
LweI GCATC 2 cut(s) 87, 940
MaeI CTAG 4 cut(s) 780, 1220, 1229, 1236
MaeII ACGT 3 cut(s) 140, 239, 254
MaeIII GTNAC 5 cut(s) 70, 136, 304, 1136, 1492
MalI GATC 6 cut(s) 686, 807, 902, 974, 1301, 1436
MboI GATC 6 cut(s) 684, 805, 900, 972, 1299, 1434
MfeI CAATTG 4 cut(s) 933, 1158, 1440, 1542
MflI RGATCY 2 cut(s) 805, 1434
MhlI GDGCHC 2 cut(s) 505, 1362
Mly113I GGCGCC 2 cut(s) 366, 482
MlyI GAGTC 3 cut(s) 143, 301, 797
Mph1103I ATGCAT 1 cut(s) 80
MroI TCCGGA 1 cut(s) 723
MroXI GAANNNNTTC 3 cut(s) 164, 566, 1046
MseI TTAA 1 cut(s) 987
MspA1I CMGCKG 1 cut(s) 1268
MunI CAATTG 4 cut(s) 933, 1158, 1440, 1542
Mva1269I GAATGC 1 cut(s) 1376
MvnI CGCG 2 cut(s) 693, 735
MwoI GCNNNNNNNGC 4 cut(s) 281, 389, 697, 1199
NarI GGCGCC 2 cut(s) 366, 482
NcoI CCATGG 3 cut(s) 90, 144, 809
NdeI CATATG 1 cut(s) 1186
NdeII GATC 6 cut(s) 684, 805, 900, 972, 1299, 1434
NlaIV GGNNCC 7 cut(s) 367, 483, 627, 652, 842, 1215, 1436
NmuCI GTSAC 1 cut(s) 304
NsiI ATGCAT 1 cut(s) 80
NspI RCATGY 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 1367
PctI GAATGC 1 cut(s) 1376
PdmI GAANNNNTTC 3 cut(s) 164, 566, 1046
PfeI GAWTC 5 cut(s) 123, 441, 730, 817, 865
PinAI ACCGGT 2 cut(s) 56, 887
PleI GAGTC 3 cut(s) 143, 301, 796
PluTI GGCGCC 2 cut(s) 369, 485
PpsI GAGTC 3 cut(s) 143, 301, 796
PpuMI RGGWCCY 1 cut(s) 1214
Psp124BI GAGCTC 1 cut(s) 505
Psp5II RGGWCCY 1 cut(s) 1214
PspN4I GGNNCC 7 cut(s) 367, 483, 627, 652, 842, 1215, 1436
PspPI GGNCC 3 cut(s) 625, 840, 1214
PspPPI RGGWCCY 1 cut(s) 1214
PsuI RGATCY 2 cut(s) 805, 1434
PvuII CAGCTG 1 cut(s) 1268
RsaI GTAC 5 cut(s) 182, 218, 836, 1409, 1478
RsaNI GTAC 5 cut(s) 181, 217, 835, 1408, 1477
SacI GAGCTC 1 cut(s) 505
SapI GCTCTTC 1 cut(s) 1367
SaqAI TTAA 1 cut(s) 987
Sau3AI GATC 6 cut(s) 684, 805, 900, 972, 1299, 1434
Sau96I GGNCC 3 cut(s) 625, 840, 1214
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 3 cut(s) 143, 301, 797
SduI GDGCHC 2 cut(s) 505, 1362
SfaNI GCATC 2 cut(s) 87, 940
SfoI GGCGCC 2 cut(s) 367, 483
SinI GGWCC 2 cut(s) 625, 1214
SmlI CTYRAG 2 cut(s) 1052, 1320
SmoI CTYRAG 2 cut(s) 1052, 1320
SsiI CCGC 7 cut(s) 27, 30, 87, 588, 693, 735, 1094
SspDI GGCGCC 2 cut(s) 365, 481
SspI AATATT 2 cut(s) 660, 1245
SspMI CTAG 4 cut(s) 780, 1220, 1229, 1236
SstI GAGCTC 1 cut(s) 505
StyI CCWWGG 5 cut(s) 90, 144, 387, 809, 1251
TaaI ACNGT 5 cut(s) 185, 233, 304, 535, 646
TaiI ACGT 3 cut(s) 143, 242, 257
TaqI TCGA 2 cut(s) 687, 1010
TatI WGTACW 3 cut(s) 216, 834, 1407
TauI GCSGC 6 cut(s) 30, 33, 89, 696, 738, 1097
TfiI GAWTC 5 cut(s) 123, 441, 730, 817, 865
Tru1I TTAA 1 cut(s) 987
Tru9I TTAA 1 cut(s) 987
TscAI CASTG 1 cut(s) 28
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 6 cut(s) 272, 296, 383, 880, 995, 1268
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 5 cut(s) 1165, 1300, 1354, 1364, 1515
TspGWI ACGGA 1 cut(s) 1387
TspRI CASTG 1 cut(s) 28
VpaK11BI GGWCC 2 cut(s) 625, 1214
XagI CCTNNNNNAGG 1 cut(s) 9
XapI RAATTY 2 cut(s) 1112, 1352
XceI RCATGY 1 cut(s) 78
XmiI GTMKAC 1 cut(s) 206
XmnI GAANNNNTTC 3 cut(s) 164, 566, 1046
XspI CTAG 4 cut(s) 780, 1220, 1229, 1236
ZrmI AGTACT 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.