Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002892

Physical Location & Seq

Genomic Coordinates Reverse (-)

46385540 .. 46391788

Gene Structure 6249 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1344300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1707 bp

ATGCCAAAAAGTACTTATGTCCACGGACAACGTGTCAAATACATTCGTTCTCTAACATGGACTTCGTACATTATTAATGCAGCCTCATACTTCCAAAATTCCAAGCACCAATTTCTCTCCATGCATCGAACTGTGGCACACTTCTGCCACACACCCCACCACCTTTTTGGTCCAAAAATGGGCTCTCAACACCCACTCCACCAACCCATCTTACACTCCGAACCTGAACCCGTAGCACAAGCCTCCAGCGATGGAGACGGTGTGGACTTTCTACTCGAGAAGGTGCTATCCGACACCCAATTGCCCTCGTTCAAGCGATTCCGCTCGGCCACATGGATTGAACTCAAGCTCCTCTTTCGTCTTGCTGCTCCCGCCGTCTTGGTTTATGTCATTAACAACTCCATGTCACTTTCCGCTCGTGTCTTTGCCGGCCACCTCGGCAATCTTGAGCTCGCCGCCGCCTCTCTCGGCAACAACGGCATCCAACTCTTGGCCTATGGCCTCATGCTAGGCATGGGAAGTGCAGTGGAGACTCTATGCGGGCAAGCTTACGGTGCCCAGAAATATGACATGTTAAGCATATACCTTCAAAGAGCAACCATAGTCCTATCTCTGACTGGCCTCCCACTTCTGGCAATCTTTCTTCTCACAAAACCCATGTTGATTTTACTCGGCGAACCGCCCGCCGTGGCCTCAGCCGCCGCCGTATTTGTCTACGGTCTAATCCCTCAAATATTTGCCTACGCCGTCAACTTCCCCATTCAAAAGTTTCTCCAAGCACAACGAATTGTGGCCCCGAGTGCTTACATATCAGCAGCTACTTTAGGAGTGCATTTGTTGCTGAGCTGGGTTGCTGTGTACAAGCTGGGTTTAGGGTTGATAGGTGCGTCTCTTGTTTTGAGCTTGTCCTGGTGGATCATAGTTGGGGCCCAGATTGTTTACATCTTGGTTAGCAGTCAGTGTAAGCTCACTTGGAATGGTTTTAGTTTGCAAGCCTTTTCTGGGTTGTGGGATTTTTTCAAGCTATCGGCTGCATCGGCGGTGATGTTGTGCTTGGAGTGTTGGTATTTTCAGGTGCTAGTTTTGATTGCTGGGTTGCTCAAGAACCCCGAGCTTGCCCTAAATTCTTTGGCCGTATGCATGTCGATAAGTGGAGTGTTGTTCAATGTTTCAGCCGGGTTCAATGCAGCTGCAAGTGTGAGGGTAAGCAATGAGCTAGGTGCTGGGAATCCAAAGTCAGCAGCATTCTCGGTTCTAGTGGTAACTTTGGTATCCTTAACTTATGCTGTTGTGGAAGCTGTGATTGTGCTCTCCCTCCGTGACGTAATTAGTTATGCCTTCACTAGCGGTGAAACTGTGTCCAAGGCAGTCTCGCACCTCACTCCATATTTGGCCGTCACTCTCATCCTCAATGGGATTCAACCAGTCCTATCCGGGGTGGCTGTTGGATGTGGGTGGCAAGCATTTGTGGCATATGTGAATGTGGGATGCTATTACGTGGTTGGAATTCCATTCGGCTGTGTTCTTGGGTTTAGATTCGACCTTGGCGCTGAGGGAATATGGGTGGGGATGATTGGAGGAACACTGATCCAAACCTTAATTTTACTGTGGATAACATTTCGTACAGACTGGAATAAAGAGGTGGACAAGGCTAAAAATCGCGTGGAGAAATGGGATGACAAGAAGCAGCCTCTGCTAAAAGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MatE

Protein Analysis

569

Amino Acids

61.62

Weight (kDa)

9.04

Isoelectric Point (pI)

24.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MatE	PF01554	124 - 284	4.3e-33	MatE
MatE	PF01554	346 - 506	1.8e-27	MatE

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002892)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11670 AT1G61890 AT1G61890 AT1G61890
fragaria_vesca	FvH4_3g02260 FvH4_3g02260
malus_domestica	MD05G1344300.v1.1 MD10G1320300.v1.1
prunus_persica	Prupe.4G022000_v2.0.a1 Prupe.4G022000_v2.0.a1 Prupe.4G022000_v2.0.a1 Prupe.4G022000_v2.0.a1 Prupe.4G022000_v2.0.a1 Prupe.4G022100_v2.0.a1 Prupe.4G022100_v2.0.a1
pyrus_communis	pycom05g31370 pycom10g27100
rosa_chinensis	RchiOBHm_Chr5g0003331 RchiOBHm_Chr5g0003381
rosa_laevigata	RLG00000031125 RLG00000031127 RLG00000031131 RLG00000031134
rosa_multiflora	Rmu_sc0006842.1_g000003 Rmu_sc0006842.1_g000010 Rmu_sc0010917.1_g000002 Rmu_sc0010917.1_g000004
rosa_roxburghii	Rroxscaffold_1G00072640
rosa_rugosa	Rorug04G0402900
rosa_samantha	Rh5AG028900 Rh5AG029500 Rh5BG029000 Rh5DG029500
rosa_wichuraiana	Rw0G014030 Rw0G015590 Rw5G002690 Rw5G002740 Rw5G002760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	554
AccB7I	CCANNNNNTGG	1 cut(s)	490
AccBSI	CCGCTC	2 cut(s)	324, 416
AccI	GTMKAC	1 cut(s)	714
AccII	CGCG	1 cut(s)	1662
AclWI	GGATC	2 cut(s)	923, 1582
AcoI	YGGCCR	4 cut(s)	327, 430, 1131, 1392
AcsI	RAATTY	3 cut(s)	97, 1123, 1506
AfaI	GTAC	4 cut(s)	13, 68, 860, 1624
AfiI	CCNNNNNNNGG	5 cut(s)	179, 490, 631, 1002, 1435
AflIII	ACRYGT	2 cut(s)	31, 570
AgsI	TTSAA	8 cut(s)	313, 341, 590, 764, 1021, 1165, 1183, 1421
AhdI	GACNNNNNGTC	1 cut(s)	32
AjnI	CCWGG	1 cut(s)	908
AloI	GAACNNNNNNTCC	2 cut(s)	333, 365
Alw21I	GWGCWC	2 cut(s)	453, 1311
Alw26I	GTCTC	4 cut(s)	249, 524, 894, 1375
AlwI	GGATC	2 cut(s)	923, 1582
AlwNI	CAGNNNCTG	1 cut(s)	1693
Ama87I	CYCGRG	3 cut(s)	275, 796, 1109
ApaI	GGGCCC	1 cut(s)	931
ApeKI	GCWGC	8 cut(s)	80, 365, 815, 1031, 1187, 1190, 1241, 1687
ApoI	RAATTY	3 cut(s)	97, 1123, 1506
AseI	ATTAAT	1 cut(s)	75
AspLEI	GCGC	1 cut(s)	1550
AspS9I	GGNCC	4 cut(s)	170, 793, 927, 928
AsuC2I	CCSGG	2 cut(s)	1177, 1435
AsuHPI	GGTGA	2 cut(s)	1054, 1361
AvaI	CYCGRG	3 cut(s)	275, 796, 1109
AvaII	GGWCC	1 cut(s)	170
BaeGI	GKGCMC	2 cut(s)	559, 931
BaeI	ACNNNNGTAYC	2 cut(s)	1254, 1287
BanI	GGYRCC	1 cut(s)	554
BanII	GRGCYC	3 cut(s)	185, 453, 931
BauI	CACGAG	1 cut(s)	417
Bbv12I	GWGCWC	2 cut(s)	453, 1311
BbvCI	CCTCAGC	2 cut(s)	694, 1551
BbvI	GCAGC	8 cut(s)	92, 352, 827, 1018, 1177, 1199, 1253, 1699
BccI	CCATC	2 cut(s)	215, 245
BceAI	ACGGC	7 cut(s)	359, 493, 671, 689, 731, 1118, 1379
BcgI	CGANNNNNNTGC	2 cut(s)	1230, 1264
BciT130I	CCWGG	1 cut(s)	910
BciVI	GTATCC	1 cut(s)	1282
BcnI	CCSGG	2 cut(s)	1177, 1435
BcoDI	GTCTC	4 cut(s)	249, 524, 894, 1375
BfaI	CTAG	5 cut(s)	509, 1079, 1217, 1256, 1344
BfoI	RGCGCY	1 cut(s)	1551
BfuI	GTATCC	1 cut(s)	1282
BglI	GCCNNNNNGGC	1 cut(s)	438
BlpI	GCTNAGC	1 cut(s)	842
BmcAI	AGTACT	1 cut(s)	13
Bme1390I	CCNGG	3 cut(s)	910, 1177, 1435
Bme18I	GGWCC	1 cut(s)	170
BmeRI	GACNNNNNGTC	1 cut(s)	32
BmeT110I	CYCGRG	3 cut(s)	275, 796, 1109
BmgT120I	GGNCC	4 cut(s)	170, 793, 927, 928
BmiI	GGNNCC	4 cut(s)	556, 795, 928, 929
BmrFI	CCNGG	3 cut(s)	910, 1177, 1435
BmsI	GCATC	4 cut(s)	133, 489, 1043, 1478
BpmI	CTGGAG	1 cut(s)	229
Bpu10I	CCTNAGC	2 cut(s)	694, 1551
Bpu1102I	GCTNAGC	1 cut(s)	842
BpuEI	CTTGAG	3 cut(s)	329, 467, 1085
BpuMI	CCSGG	2 cut(s)	1177, 1435
BsaAI	YACGTR	1 cut(s)	1498
BsaJI	CCNNGG	6 cut(s)	22, 436, 687, 1362, 1434, 1543
BsaXI	ACNNNNNCTCC	8 cut(s)	180, 210, 246, 276, 333, 363, 1049, 1079
Bsc4I	CCNNNNNNNGG	5 cut(s)	179, 490, 631, 1002, 1435
Bse118I	RCCGGY	1 cut(s)	428
Bse1I	ACTGG	3 cut(s)	622, 1424, 1634
Bse3DI	GCAATG	1 cut(s)	1216
BseBI	CCWGG	1 cut(s)	910
BseDI	CCNNGG	6 cut(s)	22, 436, 687, 1362, 1434, 1543
BseGI	GGATG	6 cut(s)	480, 1404, 1454, 1493, 1575, 1681
BseLI	CCNNNNNNNGG	5 cut(s)	179, 490, 631, 1002, 1435
BseMI	GCAATG	1 cut(s)	1216
BseMII	CTCAG	3 cut(s)	708, 833, 1542
BseNI	ACTGG	3 cut(s)	622, 1424, 1634
BseRI	GAGGAG	1 cut(s)	341
BseSI	GKGCMC	2 cut(s)	559, 931
BseXI	GCAGC	8 cut(s)	92, 352, 827, 1018, 1177, 1199, 1253, 1699
BseYI	CCCAGC	4 cut(s)	846, 865, 1091, 1223
BsgI	GTGCAG	1 cut(s)	543
Bsh1236I	CGCG	1 cut(s)	1662
BshNI	GGYRCC	1 cut(s)	554
BsiHKAI	GWGCWC	2 cut(s)	453, 1311
BsiHKCI	CYCGRG	3 cut(s)	275, 796, 1109
BsiSI	CCGG	3 cut(s)	429, 1176, 1434
BslI	CCNNNNNNNGG	5 cut(s)	179, 490, 631, 1002, 1435
BsmAI	GTCTC	4 cut(s)	249, 524, 894, 1375
BsmBI	CGTCTC	2 cut(s)	249, 894
BsmI	GAATGC	1 cut(s)	1244
BsoBI	CYCGRG	3 cut(s)	275, 796, 1109
Bsp120I	GGGCCC	1 cut(s)	927
Bsp1286I	GDGCHC	5 cut(s)	185, 453, 559, 931, 1311
Bsp1407I	TGTACA	1 cut(s)	858
Bsp143I	GATC	2 cut(s)	915, 1587
Bsp1720I	GCTNAGC	1 cut(s)	842
BspCNI	CTCAG	3 cut(s)	707, 834, 1543
BspFNI	CGCG	1 cut(s)	1662
BspLI	GGNNCC	4 cut(s)	556, 795, 928, 929
BspPI	GGATC	2 cut(s)	923, 1582
BspT107I	GGYRCC	1 cut(s)	554
BsrBI	CCGCTC	2 cut(s)	324, 416
BsrDI	GCAATG	1 cut(s)	1216
BsrFI	RCCGGY	1 cut(s)	428
BsrGI	TGTACA	1 cut(s)	858
BsrI	ACTGG	3 cut(s)	622, 1424, 1634
BssAI	RCCGGY	1 cut(s)	428
BssECI	CCNNGG	6 cut(s)	22, 436, 687, 1362, 1434, 1543
BssMI	GATC	2 cut(s)	915, 1587
BssSI	CACGAG	1 cut(s)	417
BssT1I	CCWWGG	2 cut(s)	1362, 1543
Bst2BI	CACGAG	1 cut(s)	417
Bst2UI	CCWGG	1 cut(s)	910
Bst4CI	ACNGT	6 cut(s)	133, 260, 554, 719, 1357, 1608
BstAPI	GCANNNNNTGC	2 cut(s)	838, 1693
BstAUI	TGTACA	1 cut(s)	858
BstBAI	YACGTR	1 cut(s)	1498
BstC8I	GCNNGC	8 cut(s)	430, 453, 542, 546, 684, 993, 1116, 1461
BstDEI	CTNAG	3 cut(s)	694, 842, 1551
BstDSI	CCRYGG	2 cut(s)	22, 687
BstF5I	GGATG	6 cut(s)	480, 1404, 1454, 1493, 1575, 1681
BstFNI	CGCG	1 cut(s)	1662
BstH2I	RGCGCY	1 cut(s)	1551
BstHHI	GCGC	1 cut(s)	1550
BstKTI	GATC	2 cut(s)	918, 1590
BstMAI	GTCTC	4 cut(s)	249, 524, 894, 1375
BstMBI	GATC	2 cut(s)	915, 1587
BstNI	CCWGG	1 cut(s)	910
BstNSI	RCATGY	2 cut(s)	574, 1144
BstSCI	CCNGG	3 cut(s)	908, 1175, 1433
BstSLI	GKGCMC	2 cut(s)	559, 931
BstUI	CGCG	1 cut(s)	1662
BstV1I	GCAGC	8 cut(s)	92, 352, 827, 1018, 1177, 1199, 1253, 1699
BstXI	CCANNNNNNTGG	1 cut(s)	167
BsuI	GTATCC	1 cut(s)	1282
BtgI	CCRYGG	2 cut(s)	22, 687
BtgZI	GCGATG	1 cut(s)	264
BtsCI	GGATG	6 cut(s)	480, 1404, 1454, 1493, 1575, 1681
BtsI	GCAGTG	1 cut(s)	531
BtsIMutI	CAGTG	3 cut(s)	531, 965, 1583
Cac8I	GCNNGC	8 cut(s)	430, 453, 542, 546, 684, 993, 1116, 1461
CaiI	CAGNNNCTG	1 cut(s)	1693
CfoI	GCGC	1 cut(s)	1550
Cfr10I	RCCGGY	1 cut(s)	428
Cfr13I	GGNCC	4 cut(s)	170, 793, 927, 928
CseI	GACGC	1 cut(s)	876
Csp6I	GTAC	4 cut(s)	12, 67, 859, 1623
CspCI	CAANNNNNGTGG	2 cut(s)	319, 354
CviAII	CATG	9 cut(s)	57, 121, 333, 403, 505, 514, 571, 658, 1141
CviQI	GTAC	4 cut(s)	12, 67, 859, 1623
DdeI	CTNAG	3 cut(s)	694, 842, 1551
DpnI	GATC	2 cut(s)	917, 1589
DpnII	GATC	2 cut(s)	915, 1587
DriI	GACNNNNNGTC	1 cut(s)	32
EaeI	YGGCCR	4 cut(s)	327, 430, 1131, 1392
Eam1105I	GACNNNNNGTC	1 cut(s)	32
Ecl136II	GAGCTC	1 cut(s)	451
Eco130I	CCWWGG	2 cut(s)	1362, 1543
Eco24I	GRGCYC	3 cut(s)	185, 453, 931
Eco47I	GGWCC	1 cut(s)	170
Eco53kI	GAGCTC	1 cut(s)	451
Eco88I	CYCGRG	3 cut(s)	275, 796, 1109
EcoICRI	GAGCTC	1 cut(s)	451
EcoO109I	RGGNCCY	1 cut(s)	927
EcoRI	GAATTC	1 cut(s)	1506
EcoRII	CCWGG	1 cut(s)	908
EcoT14I	CCWWGG	2 cut(s)	1362, 1543
EcoT22I	ATGCAT	2 cut(s)	126, 1142
EcoT38I	GRGCYC	3 cut(s)	185, 453, 931
ErhI	CCWWGG	2 cut(s)	1362, 1543
Esp3I	CGTCTC	2 cut(s)	249, 894
FaeI	CATG	9 cut(s)	60, 124, 336, 406, 508, 517, 574, 661, 1144
FalI	AAGNNNNNCTT	2 cut(s)	338, 370
FatI	CATG	9 cut(s)	56, 120, 332, 402, 504, 513, 570, 657, 1140
FauI	CCCGC	3 cut(s)	379, 533, 691
FauNDI	CATATG	1 cut(s)	1474
FblI	GTMKAC	1 cut(s)	714
FokI	GGATG	6 cut(s)	467, 1391, 1461, 1500, 1582, 1688
FriOI	GRGCYC	3 cut(s)	185, 453, 931
FspBI	CTAG	5 cut(s)	509, 1079, 1217, 1256, 1344
GlaI	GCGC	1 cut(s)	1549
GsaI	CCCAGC	4 cut(s)	850, 869, 1095, 1227
GsuI	CTGGAG	1 cut(s)	229
HaeII	RGCGCY	1 cut(s)	1551
HapII	CCGG	3 cut(s)	429, 1176, 1434
HgaI	GACGC	1 cut(s)	876
HhaI	GCGC	1 cut(s)	1550
Hin1II	CATG	9 cut(s)	60, 124, 336, 406, 508, 517, 574, 661, 1144
Hin6I	GCGC	1 cut(s)	1548
HinP1I	GCGC	1 cut(s)	1548
HincII	GTYRAC	1 cut(s)	751
HindII	GTYRAC	1 cut(s)	751
HindIII	AAGCTT	1 cut(s)	546
HinfI	GANTC	5 cut(s)	318, 532, 1228, 1417, 1536
HpaII	CCGG	3 cut(s)	429, 1176, 1434
HphI	GGTGA	2 cut(s)	1054, 1361
Hpy166II	GTNNAC	7 cut(s)	22, 265, 715, 751, 859, 940, 1645
Hpy188I	TCNGA	3 cut(s)	220, 292, 615
Hpy188III	TCNNGA	3 cut(s)	277, 446, 1102
Hpy8I	GTNNAC	7 cut(s)	22, 265, 715, 751, 859, 940, 1645
HpyAV	CCTTC	3 cut(s)	274, 596, 1348
HpyCH4III	ACNGT	6 cut(s)	133, 260, 554, 719, 1357, 1608
HpyCH4IV	ACGT	3 cut(s)	31, 1323, 1497
HpyCH4V	TGCA	9 cut(s)	80, 124, 524, 832, 991, 1034, 1140, 1187, 1193
HpyF3I	CTNAG	3 cut(s)	694, 842, 1551
HpySE526I	ACGT	3 cut(s)	31, 1323, 1497
Hsp92II	CATG	9 cut(s)	60, 124, 336, 406, 508, 517, 574, 661, 1144
HspAI	GCGC	1 cut(s)	1548
KroI	GCCGGC	1 cut(s)	428
KroNI	GCCGGC	1 cut(s)	430
Kzo9I	GATC	2 cut(s)	915, 1587
LmnI	GCTCC	2 cut(s)	354, 373
Lsp1109I	GCAGC	8 cut(s)	92, 352, 827, 1018, 1177, 1199, 1253, 1699
LweI	GCATC	4 cut(s)	133, 489, 1043, 1478
MaeI	CTAG	5 cut(s)	509, 1079, 1217, 1256, 1344
MaeII	ACGT	3 cut(s)	31, 1323, 1497
MaeIII	GTNAC	4 cut(s)	405, 1261, 1319, 1396
MalI	GATC	2 cut(s)	917, 1589
MbiI	CCGCTC	2 cut(s)	324, 416
MboI	GATC	2 cut(s)	915, 1587
MboII	GAAGA	1 cut(s)	635
MfeI	CAATTG	1 cut(s)	299
MhlI	GDGCHC	5 cut(s)	185, 453, 559, 931, 1311
MluCI	AATT	8 cut(s)	97, 110, 299, 786, 1123, 1326, 1506, 1599
MlyI	GAGTC	1 cut(s)	526
MmeI	TCCRAC	4 cut(s)	315, 508, 1426, 1483
Mph1103I	ATGCAT	2 cut(s)	126, 1142
MroNI	GCCGGC	1 cut(s)	428
MseI	TTAA	5 cut(s)	75, 393, 575, 1277, 1598
MspA1I	CMGCKG	1 cut(s)	1190
MspI	CCGG	3 cut(s)	429, 1176, 1434
MspR9I	CCNGG	3 cut(s)	910, 1177, 1435
MunI	CAATTG	1 cut(s)	299
Mva1269I	GAATGC	1 cut(s)	1244
MvaI	CCWGG	1 cut(s)	910
MvnI	CGCG	1 cut(s)	1662
NaeI	GCCGGC	1 cut(s)	430
NciI	CCSGG	2 cut(s)	1177, 1435
NdeI	CATATG	1 cut(s)	1474
NdeII	GATC	2 cut(s)	915, 1587
NgoMIV	GCCGGC	1 cut(s)	428
NlaIII	CATG	9 cut(s)	60, 124, 336, 406, 508, 517, 574, 661, 1144
NlaIV	GGNNCC	4 cut(s)	556, 795, 928, 929
NmeAIII	GCCGAG	4 cut(s)	305, 417, 447, 651
NmuCI	GTSAC	3 cut(s)	405, 1319, 1396
NsiI	ATGCAT	2 cut(s)	126, 1142
NspI	RCATGY	2 cut(s)	574, 1144
PaeR7I	CTCGAG	1 cut(s)	275
PciI	ACATGT	1 cut(s)	570
PctI	GAATGC	1 cut(s)	1244
PdiI	GCCGGC	1 cut(s)	430
PfeI	GAWTC	4 cut(s)	318, 1228, 1417, 1536
PflMI	CCANNNNNTGG	1 cut(s)	490
PleI	GAGTC	1 cut(s)	526
PpsI	GAGTC	1 cut(s)	526
Ppu21I	YACGTR	1 cut(s)	1498
PscI	ACATGT	1 cut(s)	570
PshBI	ATTAAT	1 cut(s)	75
Psp124BI	GAGCTC	1 cut(s)	453
Psp6I	CCWGG	1 cut(s)	908
PspFI	CCCAGC	4 cut(s)	846, 865, 1091, 1223
PspGI	CCWGG	1 cut(s)	908
PspN4I	GGNNCC	4 cut(s)	556, 795, 928, 929
PspOMI	GGGCCC	1 cut(s)	927
PspPI	GGNCC	4 cut(s)	170, 793, 927, 928
PstNI	CAGNNNCTG	1 cut(s)	1693
PvuII	CAGCTG	1 cut(s)	1190
RsaI	GTAC	4 cut(s)	13, 68, 860, 1624
RsaNI	GTAC	4 cut(s)	12, 67, 859, 1623
SacI	GAGCTC	1 cut(s)	453
SaqAI	TTAA	5 cut(s)	75, 393, 575, 1277, 1598
Sau3AI	GATC	2 cut(s)	915, 1587
Sau96I	GGNCC	4 cut(s)	170, 793, 927, 928
ScaI	AGTACT	1 cut(s)	13
SchI	GAGTC	1 cut(s)	526
ScrFI	CCNGG	3 cut(s)	910, 1177, 1435
SduI	GDGCHC	5 cut(s)	185, 453, 559, 931, 1311
SfaNI	GCATC	4 cut(s)	133, 489, 1043, 1478
Sfr274I	CTCGAG	1 cut(s)	275
SinI	GGWCC	1 cut(s)	170
SlaI	CTCGAG	1 cut(s)	275
SmlI	CTYRAG	4 cut(s)	275, 344, 446, 1100
SmoI	CTYRAG	4 cut(s)	275, 344, 446, 1100
Sse9I	AATT	8 cut(s)	97, 110, 299, 786, 1123, 1326, 1506, 1599
SspI	AATATT	1 cut(s)	735
SspMI	CTAG	5 cut(s)	509, 1079, 1217, 1256, 1344
SstI	GAGCTC	1 cut(s)	453
StyD4I	CCNGG	3 cut(s)	908, 1175, 1433
StyI	CCWWGG	2 cut(s)	1362, 1543
TaaI	ACNGT	6 cut(s)	133, 260, 554, 719, 1357, 1608
TaiI	ACGT	3 cut(s)	34, 1326, 1500
TaqI	TCGA	4 cut(s)	127, 276, 1145, 1539
TasI	AATT	8 cut(s)	97, 110, 299, 786, 1123, 1326, 1506, 1599
TatI	WGTACW	2 cut(s)	11, 858
TauI	GCSGC	4 cut(s)	458, 461, 701, 704
TfiI	GAWTC	4 cut(s)	318, 1228, 1417, 1536
Tru1I	TTAA	5 cut(s)	75, 393, 575, 1277, 1598
Tru9I	TTAA	5 cut(s)	75, 393, 575, 1277, 1598
TscAI	CASTG	3 cut(s)	531, 965, 1590
TseFI	GTSAC	3 cut(s)	405, 1319, 1396
TseI	GCWGC	8 cut(s)	80, 365, 815, 1031, 1187, 1190, 1241, 1687
Tsp45I	GTSAC	3 cut(s)	405, 1319, 1396
TspGWI	ACGGA	2 cut(s)	39, 1307
TspRI	CASTG	3 cut(s)	531, 965, 1590
Van91I	CCANNNNNTGG	1 cut(s)	490
VpaK11BI	GGWCC	1 cut(s)	170
VspI	ATTAAT	1 cut(s)	75
XapI	RAATTY	3 cut(s)	97, 1123, 1506
XceI	RCATGY	2 cut(s)	574, 1144
XcmI	CCANNNNNNNNNTGG	1 cut(s)	164
XhoI	CTCGAG	1 cut(s)	275
XmiI	GTMKAC	1 cut(s)	714
XspI	CTAG	5 cut(s)	509, 1079, 1217, 1256, 1344
ZrmI	AGTACT	1 cut(s)	13
Zsp2I	ATGCAT	2 cut(s)	126, 1142

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.