Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0002892

Physical Location & Seq

Genomic Coordinates Forward (+)

2130791 .. 2134837

Gene Structure 4047 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG029500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1269 bp

ATGGGTTCTCAAGAAGAGCTCCACCAACCCATCTTAAGCTCAGAACCAGAACCCGAATCCGTATCAGTAGCCTCCGCTAGTCATGGCGAGGAGTCATCTCCACTTGAGAAGGTACTATCCGACGCCCAGTTGCCGTGTTTCAAGAGGCTCCGATTGGCCACATGGATTGAACTCAAGCTCCTCTTCCGCCTTGCTGCTCCTGCCGTCTTCGTTTACGTCATCAACAACACTATGTCACTTTCCACACGTGTCTTCGCCGGCCACCTGGGTAATCTCGAGCTTGCAGCTGCCTCCCTCGGCAATAGCGGCATCCAACTCTTTGCCTACGGCCTCATGTTAGGTATGGGAAGCGCGGTGGAGACTCTATGTGGGCAAGCCTACGGTGCCCACAAGTACGACATGTTAGGCATATATCTCCAAAGAGCAACCATAGTCCTAACCATCACCGGACTTCCACTTCTTGCAGTCTTCATCCTATGCAAGCCCTTATTGCTTTTAATCGGAGAATCATCAGCCTTAGCCTCTGCAGCCGCAGTTTTCGTCTACGGTCTGATCCCACAAATCTTCGCTTACGCTGTCAATTTTCCAATCCAAAAGTTTCTCCAGGCCCAGAGAATTGTACTTCCAAGTGCCTACATATCTGCGGCTACGCTTGGGGTGCATTTATTGCTAAGTTGGGTGGCTGTATACGAGTTAGGTTGGGGGTTGATTGGTGCCTCGTTGGTTTTGAGCTTGTCTTGGTGGATCATTGTAATTGCTCAGTTCGTCTACATTTTGACAAGTAGCAAATGTAAGCAGACATGGAGTGGGTTTAGTTTGCAGGCGTTTTCGGGGTTGCGGGAGTTCCTGAAGTTATCGGCTGCATCGGCTGTGATGTTGTGCTTGGAGGTGTGGTACTTTCAGGTGCTAGTGTTGATTGCTGGCTTACTAAAGGACCCTGAGCTTGCTTTGAACTCTTTGGCTGTATGCATGGCGATAAATGGAGTATTGTTCATGGTTTCAGTTGGATTTAACGCAGCTATAAGTGTGCGAGTAAGCAACGAGCTAGGTGCTGGGAATCCAAAGTCTGCAGCATTCTCGGTTGTGATAGTAACACTAGTGTCGTTGGCTATTGCTATAGTTGAAGCCGTCATTGTGCTGTTGCTCCGCGATGTAATCAGTTACGCCTTTACCAGTGGCGAAACTGTTGCCGAAGCAGTCTCTAAACTCACTCCGTATTTGGCCGTCACTCTAATCCTTAATGGGATTCAACCTGTCCTCTCGGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MatE

Protein Analysis

422

Amino Acids

45.03

Weight (kDa)

5.99

Isoelectric Point (pI)

25.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MatE	PF01554	67 - 227	5.4e-34	MatE
MatE	PF01554	289 - 422	1.2e-22	MatE

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002892)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11670 AT1G61890 AT1G61890 AT1G61890
fragaria_vesca	FvH4_3g02260 FvH4_3g02260
malus_domestica	MD05G1344300.v1.1 MD10G1320300.v1.1
prunus_persica	Prupe.4G022000_v2.0.a1 Prupe.4G022000_v2.0.a1 Prupe.4G022000_v2.0.a1 Prupe.4G022000_v2.0.a1 Prupe.4G022000_v2.0.a1 Prupe.4G022100_v2.0.a1 Prupe.4G022100_v2.0.a1
pyrus_communis	pycom05g31370 pycom10g27100
rosa_chinensis	RchiOBHm_Chr5g0003331 RchiOBHm_Chr5g0003381
rosa_laevigata	RLG00000031125 RLG00000031127 RLG00000031131 RLG00000031134
rosa_multiflora	Rmu_sc0006842.1_g000003 Rmu_sc0006842.1_g000010 Rmu_sc0010917.1_g000002 Rmu_sc0010917.1_g000004
rosa_roxburghii	Rroxscaffold_1G00072640
rosa_rugosa	Rorug04G0402900
rosa_samantha	Rh5AG028900 Rh5AG029500 Rh5BG029000 Rh5DG029500
rosa_wichuraiana	Rw0G014030 Rw0G015590 Rw5G002690 Rw5G002740 Rw5G002760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	383, 713
AccI	GTMKAC	3 cut(s)	543, 687, 768
AccII	CGCG	2 cut(s)	353, 1149
AciI	CCGC	8 cut(s)	75, 187, 306, 353, 531, 644, 838, 1147
AclWI	GGATC	2 cut(s)	547, 752
AcoI	YGGCCR	3 cut(s)	156, 259, 1221
AcuI	CTGAAG	1 cut(s)	869
AcvI	CACGTG	1 cut(s)	248
AcyI	GRCGYC	1 cut(s)	123
AfaI	GTAC	4 cut(s)	114, 395, 621, 896
AflII	CTTAAG	1 cut(s)	34
AflIII	ACRYGT	3 cut(s)	245, 247, 399
AgsI	TTSAA	5 cut(s)	142, 170, 952, 1124, 1250
AhlI	ACTAGT	1 cut(s)	1096
AjnI	CCWGG	2 cut(s)	264, 603
AloI	GAACNNNNNNTCC	2 cut(s)	162, 194
AluBI	AGCT	9 cut(s)	19, 39, 178, 280, 287, 732, 943, 1019, 1045
AluI	AGCT	9 cut(s)	19, 39, 178, 280, 287, 732, 943, 1019, 1045
Alw21I	GWGCWC	1 cut(s)	21
Alw26I	GTCTC	2 cut(s)	353, 1204
AlwI	GGATC	2 cut(s)	547, 752
Ama87I	CYCGRG	2 cut(s)	275, 1261
AoxI	GGCC	5 cut(s)	156, 259, 328, 606, 1221
ApeKI	GCWGC	7 cut(s)	194, 284, 287, 527, 860, 1016, 1070
AspLEI	GCGC	1 cut(s)	353
AspS9I	GGNCC	2 cut(s)	607, 934
AsuHPI	GGTGA	1 cut(s)	436
AvaI	CYCGRG	2 cut(s)	275, 1261
AvaII	GGWCC	1 cut(s)	934
BaeGI	GKGCMC	1 cut(s)	388
BalI	TGGCCA	1 cut(s)	158
BanI	GGYRCC	2 cut(s)	383, 713
BanII	GRGCYC	1 cut(s)	21
BbrPI	CACGTG	1 cut(s)	248
BbsI	GAAGAC	3 cut(s)	199, 244, 460
Bbv12I	GWGCWC	1 cut(s)	21
BbvI	GCAGC	7 cut(s)	181, 274, 296, 539, 847, 1028, 1082
BccI	CCATC	2 cut(s)	38, 449
BceAI	ACGGC	5 cut(s)	118, 188, 343, 1112, 1208
BcgI	CGANNNNNNTGC	6 cut(s)	1031, 1059, 1065, 1093, 1169, 1203
BciT130I	CCWGG	2 cut(s)	266, 605
BcoDI	GTCTC	2 cut(s)	353, 1204
BcuI	ACTAGT	1 cut(s)	1096
BfaI	CTAG	4 cut(s)	78, 908, 1046, 1097
BfmI	CTRYAG	3 cut(s)	525, 1068, 1116
BfrI	CTTAAG	1 cut(s)	34
Bme1390I	CCNGG	2 cut(s)	266, 605
Bme18I	GGWCC	1 cut(s)	934
BmeT110I	CYCGRG	2 cut(s)	275, 1261
BmgT120I	GGNCC	2 cut(s)	607, 934
BmiI	GGNNCC	4 cut(s)	149, 385, 715, 936
BmrFI	CCNGG	2 cut(s)	266, 605
BmrI	ACTGGG	1 cut(s)	121
BmsI	GCATC	2 cut(s)	318, 872
BmuI	ACTGGG	1 cut(s)	121
BpiI	GAAGAC	3 cut(s)	199, 244, 460
BpmI	CTGGAG	1 cut(s)	587
Bpu10I	CCTNAGC	2 cut(s)	517, 939
BpuEI	CTTGAG	2 cut(s)	125, 158
BsaAI	YACGTR	1 cut(s)	248
BsaHI	GRCGYC	1 cut(s)	123
BsaJI	CCNNGG	2 cut(s)	265, 295
BsaWI	WCCGGW	1 cut(s)	446
BsaXI	ACNNNNNCTCC	4 cut(s)	162, 192, 878, 908
Bse118I	RCCGGY	1 cut(s)	257
Bse1I	ACTGG	2 cut(s)	127, 1173
BseBI	CCWGG	2 cut(s)	266, 605
BseDI	CCNNGG	2 cut(s)	265, 295
BseGI	GGATG	2 cut(s)	309, 471
BseMII	CTCAG	3 cut(s)	54, 773, 930
BseNI	ACTGG	2 cut(s)	127, 1173
BseRI	GAGGAG	2 cut(s)	104, 170
BseSI	GKGCMC	1 cut(s)	388
BseXI	GCAGC	7 cut(s)	181, 274, 296, 539, 847, 1028, 1082
BseYI	CCCAGC	1 cut(s)	1052
Bsh1236I	CGCG	2 cut(s)	353, 1149
BshFI	GGCC	5 cut(s)	158, 261, 330, 608, 1223
BshNI	GGYRCC	2 cut(s)	383, 713
BsiHKAI	GWGCWC	1 cut(s)	21
BsiHKCI	CYCGRG	2 cut(s)	275, 1261
BsiSI	CCGG	2 cut(s)	258, 447
BsmAI	GTCTC	2 cut(s)	353, 1204
BsmI	GAATGC	1 cut(s)	1073
BsnI	GGCC	5 cut(s)	158, 261, 330, 608, 1223
BsoBI	CYCGRG	2 cut(s)	275, 1261
Bsp1286I	GDGCHC	2 cut(s)	21, 388
Bsp143I	GATC	2 cut(s)	552, 744
BspACI	CCGC	8 cut(s)	75, 187, 306, 353, 531, 644, 838, 1147
BspANI	GGCC	5 cut(s)	158, 261, 330, 608, 1223
BspCNI	CTCAG	3 cut(s)	53, 772, 931
BspFNI	CGCG	2 cut(s)	353, 1149
BspLI	GGNNCC	4 cut(s)	149, 385, 715, 936
BspMAI	CTGCAG	2 cut(s)	529, 1072
BspPI	GGATC	2 cut(s)	547, 752
BspQI	GCTCTTC	1 cut(s)	9
BspT107I	GGYRCC	2 cut(s)	383, 713
BspTI	CTTAAG	1 cut(s)	34
BsrFI	RCCGGY	1 cut(s)	257
BsrI	ACTGG	2 cut(s)	127, 1173
BssAI	RCCGGY	1 cut(s)	257
BssECI	CCNNGG	2 cut(s)	265, 295
BssMI	GATC	2 cut(s)	552, 744
BssNAI	GTATAC	1 cut(s)	688
BssNI	GRCGYC	1 cut(s)	123
Bst1107I	GTATAC	1 cut(s)	688
Bst2UI	CCWGG	2 cut(s)	266, 605
Bst4CI	ACNGT	3 cut(s)	383, 548, 1186
Bst6I	CTCTTC	2 cut(s)	9, 188
BstACI	GRCGYC	1 cut(s)	123
BstAFI	CTTAAG	1 cut(s)	34
BstAPI	GCANNNNNTGC	1 cut(s)	667
BstBAI	YACGTR	1 cut(s)	248
BstC8I	GCNNGC	7 cut(s)	259, 282, 375, 482, 822, 922, 945
BstDEI	CTNAG	5 cut(s)	40, 517, 671, 759, 939
BstF5I	GGATG	2 cut(s)	309, 471
BstFNI	CGCG	2 cut(s)	353, 1149
BstHHI	GCGC	1 cut(s)	353
BstKTI	GATC	2 cut(s)	555, 747
BstMAI	GTCTC	2 cut(s)	353, 1204
BstMBI	GATC	2 cut(s)	552, 744
BstMWI	GCNNNNNNNGC	8 cut(s)	200, 306, 383, 490, 527, 658, 667, 866
BstNI	CCWGG	2 cut(s)	266, 605
BstNSI	RCATGY	1 cut(s)	403
BstSCI	CCNGG	2 cut(s)	264, 603
BstSFI	CTRYAG	3 cut(s)	525, 1068, 1116
BstSLI	GKGCMC	1 cut(s)	388
BstUI	CGCG	2 cut(s)	353, 1149
BstV1I	GCAGC	7 cut(s)	181, 274, 296, 539, 847, 1028, 1082
BstV2I	GAAGAC	3 cut(s)	199, 244, 460
BstZ17I	GTATAC	1 cut(s)	688
BsuRI	GGCC	5 cut(s)	158, 261, 330, 608, 1223
BtgZI	GCGATG	1 cut(s)	1164
BtsCI	GGATG	2 cut(s)	309, 471
BtsIMutI	CAGTG	1 cut(s)	1180
Cac8I	GCNNGC	7 cut(s)	259, 282, 375, 482, 822, 922, 945
CfoI	GCGC	1 cut(s)	353
Cfr10I	RCCGGY	1 cut(s)	257
Cfr13I	GGNCC	2 cut(s)	607, 934
CseI	GACGC	1 cut(s)	131
Csp6I	GTAC	4 cut(s)	113, 394, 620, 895
CspCI	CAANNNNNGTGG	2 cut(s)	148, 183
CviAII	CATG	7 cut(s)	83, 162, 334, 400, 801, 970, 994
CviQI	GTAC	4 cut(s)	113, 394, 620, 895
DdeI	CTNAG	5 cut(s)	40, 517, 671, 759, 939
DpnI	GATC	2 cut(s)	554, 746
DpnII	GATC	2 cut(s)	552, 744
EaeI	YGGCCR	3 cut(s)	156, 259, 1221
Eam1104I	CTCTTC	2 cut(s)	9, 188
EarI	CTCTTC	2 cut(s)	9, 188
EciI	GGCGGA	1 cut(s)	176
Ecl136II	GAGCTC	1 cut(s)	19
Eco24I	GRGCYC	1 cut(s)	21
Eco47I	GGWCC	1 cut(s)	934
Eco53kI	GAGCTC	1 cut(s)	19
Eco57I	CTGAAG	1 cut(s)	869
Eco72I	CACGTG	1 cut(s)	248
Eco88I	CYCGRG	2 cut(s)	275, 1261
EcoICRI	GAGCTC	1 cut(s)	19
EcoO109I	RGGNCCY	1 cut(s)	934
EcoRII	CCWGG	2 cut(s)	264, 603
EcoT22I	ATGCAT	1 cut(s)	971
EcoT38I	GRGCYC	1 cut(s)	21
FaeI	CATG	7 cut(s)	86, 165, 337, 403, 804, 973, 997
FalI	AAGNNNNNCTT	2 cut(s)	167, 199
FatI	CATG	7 cut(s)	82, 161, 333, 399, 800, 969, 993
FauI	CCCGC	1 cut(s)	831
FblI	GTMKAC	3 cut(s)	543, 687, 768
FokI	GGATG	2 cut(s)	296, 458
FriOI	GRGCYC	1 cut(s)	21
FspBI	CTAG	4 cut(s)	78, 908, 1046, 1097
GlaI	GCGC	1 cut(s)	352
GsaI	CCCAGC	1 cut(s)	1056
GsuI	CTGGAG	1 cut(s)	587
HaeIII	GGCC	5 cut(s)	158, 261, 330, 608, 1223
HapII	CCGG	2 cut(s)	258, 447
HgaI	GACGC	1 cut(s)	131
HhaI	GCGC	1 cut(s)	353
Hin1I	GRCGYC	1 cut(s)	123
Hin1II	CATG	7 cut(s)	86, 165, 337, 403, 804, 973, 997
Hin6I	GCGC	1 cut(s)	351
HinP1I	GCGC	1 cut(s)	351
HinfI	GANTC	6 cut(s)	56, 92, 361, 506, 1057, 1246
HpaII	CCGG	2 cut(s)	258, 447
HphI	GGTGA	1 cut(s)	436
Hpy166II	GTNNAC	4 cut(s)	214, 544, 688, 769
Hpy188I	TCNGA	5 cut(s)	43, 121, 152, 503, 552
Hpy188III	TCNNGA	4 cut(s)	11, 142, 275, 847
Hpy8I	GTNNAC	4 cut(s)	214, 544, 688, 769
Hpy99I	CGWCG	1 cut(s)	125
HpyAV	CCTTC	1 cut(s)	103
HpyCH4III	ACNGT	3 cut(s)	383, 548, 1186
HpyCH4IV	ACGT	2 cut(s)	216, 247
HpyCH4V	TGCA	9 cut(s)	284, 464, 480, 527, 661, 820, 863, 969, 1070
HpyF10VI	GCNNNNNNNGC	8 cut(s)	200, 306, 383, 490, 527, 658, 667, 866
HpyF3I	CTNAG	5 cut(s)	40, 517, 671, 759, 939
HpySE526I	ACGT	2 cut(s)	216, 247
Hsp92I	GRCGYC	1 cut(s)	123
Hsp92II	CATG	7 cut(s)	86, 165, 337, 403, 804, 973, 997
HspAI	GCGC	1 cut(s)	351
KroI	GCCGGC	1 cut(s)	257
KroNI	GCCGGC	1 cut(s)	259
Kzo9I	GATC	2 cut(s)	552, 744
LguI	GCTCTTC	1 cut(s)	9
LmnI	GCTCC	5 cut(s)	24, 153, 183, 202, 1149
Lsp1109I	GCAGC	7 cut(s)	181, 274, 296, 539, 847, 1028, 1082
LweI	GCATC	2 cut(s)	318, 872
MaeI	CTAG	4 cut(s)	78, 908, 1046, 1097
MaeII	ACGT	2 cut(s)	216, 247
MaeIII	GTNAC	4 cut(s)	234, 1090, 1160, 1225
MalI	GATC	2 cut(s)	554, 746
MboI	GATC	2 cut(s)	552, 744
MboII	GAAGA	6 cut(s)	26, 175, 199, 244, 460, 556
MhlI	GDGCHC	2 cut(s)	21, 388
MlsI	TGGCCA	1 cut(s)	158
MluCI	AATT	3 cut(s)	580, 615, 753
MluNI	TGGCCA	1 cut(s)	158
MlyI	GAGTC	2 cut(s)	101, 355
MmeI	TCCRAC	3 cut(s)	144, 337, 985
Mox20I	TGGCCA	1 cut(s)	158
Mph1103I	ATGCAT	1 cut(s)	971
MroNI	GCCGGC	1 cut(s)	257
MscI	TGGCCA	1 cut(s)	158
MseI	TTAA	4 cut(s)	35, 497, 1011, 1239
Msp20I	TGGCCA	1 cut(s)	158
MspA1I	CMGCKG	1 cut(s)	287
MspCI	CTTAAG	1 cut(s)	34
MspI	CCGG	2 cut(s)	258, 447
MspR9I	CCNGG	2 cut(s)	266, 605
Mva1269I	GAATGC	1 cut(s)	1073
MvaI	CCWGG	2 cut(s)	266, 605
MvnI	CGCG	2 cut(s)	353, 1149
MwoI	GCNNNNNNNGC	8 cut(s)	200, 306, 383, 490, 527, 658, 667, 866
NaeI	GCCGGC	1 cut(s)	259
NdeII	GATC	2 cut(s)	552, 744
NgoMIV	GCCGGC	1 cut(s)	257
NlaIII	CATG	7 cut(s)	86, 165, 337, 403, 804, 973, 997
NlaIV	GGNNCC	4 cut(s)	149, 385, 715, 936
NmeAIII	GCCGAG	1 cut(s)	276
NmuCI	GTSAC	2 cut(s)	234, 1225
NsiI	ATGCAT	1 cut(s)	971
NspI	RCATGY	1 cut(s)	403
PaeR7I	CTCGAG	1 cut(s)	275
PciI	ACATGT	1 cut(s)	399
PciSI	GCTCTTC	1 cut(s)	9
PctI	GAATGC	1 cut(s)	1073
PdiI	GCCGGC	1 cut(s)	259
PfeI	GAWTC	4 cut(s)	56, 506, 1057, 1246
PleI	GAGTC	2 cut(s)	100, 355
PmaCI	CACGTG	1 cut(s)	248
PmlI	CACGTG	1 cut(s)	248
PpsI	GAGTC	2 cut(s)	100, 355
Ppu21I	YACGTR	1 cut(s)	248
PpuMI	RGGWCCY	1 cut(s)	934
PscI	ACATGT	1 cut(s)	399
Psp124BI	GAGCTC	1 cut(s)	21
Psp5II	RGGWCCY	1 cut(s)	934
Psp6I	CCWGG	2 cut(s)	264, 603
PspCI	CACGTG	1 cut(s)	248
PspFI	CCCAGC	1 cut(s)	1052
PspGI	CCWGG	2 cut(s)	264, 603
PspN4I	GGNNCC	4 cut(s)	149, 385, 715, 936
PspPI	GGNCC	2 cut(s)	607, 934
PspPPI	RGGWCCY	1 cut(s)	934
PstI	CTGCAG	2 cut(s)	529, 1072
PvuII	CAGCTG	1 cut(s)	287
RsaI	GTAC	4 cut(s)	114, 395, 621, 896
RsaNI	GTAC	4 cut(s)	113, 394, 620, 895
SacI	GAGCTC	1 cut(s)	21
SapI	GCTCTTC	1 cut(s)	9
SaqAI	TTAA	4 cut(s)	35, 497, 1011, 1239
Sau3AI	GATC	2 cut(s)	552, 744
Sau96I	GGNCC	2 cut(s)	607, 934
SchI	GAGTC	2 cut(s)	101, 355
ScrFI	CCNGG	2 cut(s)	266, 605
SduI	GDGCHC	2 cut(s)	21, 388
SfaNI	GCATC	2 cut(s)	318, 872
SfcI	CTRYAG	3 cut(s)	525, 1068, 1116
Sfr274I	CTCGAG	1 cut(s)	275
SinI	GGWCC	1 cut(s)	934
SlaI	CTCGAG	1 cut(s)	275
SmlI	CTYRAG	5 cut(s)	9, 34, 104, 173, 275
SmoI	CTYRAG	5 cut(s)	9, 34, 104, 173, 275
SpeI	ACTAGT	1 cut(s)	1096
Sse9I	AATT	3 cut(s)	580, 615, 753
SsiI	CCGC	8 cut(s)	75, 187, 306, 353, 531, 644, 838, 1147
SspMI	CTAG	4 cut(s)	78, 908, 1046, 1097
SstI	GAGCTC	1 cut(s)	21
StyD4I	CCNGG	2 cut(s)	264, 603
TaaI	ACNGT	3 cut(s)	383, 548, 1186
TaiI	ACGT	2 cut(s)	219, 250
TaqI	TCGA	1 cut(s)	276
TasI	AATT	3 cut(s)	580, 615, 753
TatI	WGTACW	1 cut(s)	619
TauI	GCSGC	3 cut(s)	309, 533, 647
TfiI	GAWTC	4 cut(s)	56, 506, 1057, 1246
Tru1I	TTAA	4 cut(s)	35, 497, 1011, 1239
Tru9I	TTAA	4 cut(s)	35, 497, 1011, 1239
TscAI	CASTG	1 cut(s)	1180
TseFI	GTSAC	2 cut(s)	234, 1225
TseI	GCWGC	7 cut(s)	194, 284, 287, 527, 860, 1016, 1070
Tsp45I	GTSAC	2 cut(s)	234, 1225
TspDTI	ATGAA	2 cut(s)	460, 982
TspGWI	ACGGA	2 cut(s)	49, 1203
TspRI	CASTG	1 cut(s)	1180
Vha464I	CTTAAG	1 cut(s)	34
VpaK11BI	GGWCC	1 cut(s)	934
XceI	RCATGY	1 cut(s)	403
XhoI	CTCGAG	1 cut(s)	275
XmiI	GTMKAC	3 cut(s)	543, 687, 768
XspI	CTAG	4 cut(s)	78, 908, 1046, 1097
Zsp2I	ATGCAT	1 cut(s)	971

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.