MD05G1362400.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012091
Physical Location & Seq
Forward (+)
47755983 .. 47758015
2033 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1362400.v1.1.491

Sequence Viewer

Length: 993 bp
ATGTCGTTGAAGTCCAAAGCAGATCGGGAATGGGGTAGTACCAGTACTAGTGGCGCCGGAAGTACTAGTACCGGGTACCGCGATGATCCGCAGCCGCAGCATGATGAAGATGACGACGACGAGTACAGAAATTACTACATGAAGTACAACATATCAAGCAGTAGCATATCCGAGATTGAGGAAGAGGAAGGTGATGACATCGATGATGAGAAGAAGGGTAAGATTAAAATACCAAGCGAGATCAAAGAGCTTTTCGGATTCAAAGGGTACCCGCGGCCGATGCCCTCCATCAAAGAAGATATCGATGATCATAGCAGCCACGATGAGAGCGTTTATGTGGGAGTAGGGAAGGGCGAGTCCAGCATGGACGCGCTGACGTGGTCATTAAAGCACGCGGTGGATCCGTCATCGTCAACCACCGTCTATCTCATACATGTTTTTCCGGAGGTCACCTACATTCCCAGTCCATTGGGAAAGCTTCCAAAAAACCAAGTGACCTCACAACAGGTAGAAACTTACATGGCCGAGGAAAGAGGCAAGAGGAGAGTGCTCCTTCACAAGTTCATTGATAAATGCTCAGCTGCAAAGGTTAAGGTGGATACAATACTGATTGAAAGTGATATGATCGGAAGGGCTATTTTGGACCTCATCCCTATTCTCAACATAACAAAGCTTGTCATTGGAACTAACAGATCTAATCTAAGGAAAGTTATGTCTAAGAAAGGCAGCGGGATTGGTGCTCAAATACTTCAAAGTGCACCCGAAGGCTGTGAGGTAAACATCATATGCCAAGGAAACCCAGTGACAAAGACGATGATGGATCAGCCTCAGCCACCGACTGAGTCGTCGTTCTCCCCACCTCCCAAGGACGGTACAACGACGATTATTAGTACTACTAATAGAACTACCAACTCTATGCAATTGCAAGGAGACCAACATCAGAGGACTGAAAAGCCGGCCTCTTCATTCTTCTGTTTTAAACCCAAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

331

Amino Acids

36.58

Weight (kDa)

5.5

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012091)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47740 AT5G47740
fragaria_vesca FvH4_3g00270 FvH4_3g00270 FvH4_3g00270
malus_domestica MD05G1362400.v1.1
prunus_persica Prupe.4G003000_v2.0.a1
pyrus_communis pycom05g32880
rosa_chinensis RchiOBHm_Chr5g0000401
rosa_laevigata RLG00000030863
rosa_multiflora Rmu_sc0017532.1_g000001 Rmu_ssc0000181.1_g000001
rosa_roxburghii Rroxscaffold_1G00075760
rosa_rugosa Rorug04G0383300
rosa_samantha Rh5AG003100 Rh5BG004300 Rh5CG003300 Rh5DG003300
rosa_wichuraiana Rw5G000320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 844
Acc65I GGTACC 2 cut(s) 75, 267
AccB1I GGYRCC 3 cut(s) 53, 75, 267
AccII CGCG 4 cut(s) 81, 274, 371, 395
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 7 cut(s) 79, 89, 95, 272, 274, 395, 729
AclWI GGATC 4 cut(s) 80, 395, 408, 828
AcoI YGGCCR 2 cut(s) 275, 522
AcyI GRCGYC 1 cut(s) 54
AdeI CACNNNGTG 1 cut(s) 397
AfiI CCNNNNNNNGG 1 cut(s) 869
AflIII ACRYGT 1 cut(s) 433
AgsI TTSAA 4 cut(s) 10, 262, 614, 752
AhlI ACTAGT 2 cut(s) 47, 65
AjiI CACGTC 1 cut(s) 378
AluBI AGCT 4 cut(s) 250, 478, 581, 673
AluI AGCT 4 cut(s) 250, 478, 581, 673
Alw21I GWGCWC 3 cut(s) 552, 742, 760
Alw26I GTCTC 1 cut(s) 924
Alw44I GTGCAC 1 cut(s) 756
AlwI GGATC 4 cut(s) 80, 395, 408, 828
Aor13HI TCCGGA 1 cut(s) 442
AoxI GGCC 3 cut(s) 275, 522, 957
ApaLI GTGCAC 1 cut(s) 756
ApeKI GCWGC 5 cut(s) 91, 97, 315, 581, 726
Asp718I GGTACC 2 cut(s) 75, 267
AspLEI GCGC 2 cut(s) 56, 373
AspS9I GGNCC 1 cut(s) 643
AsuC2I CCSGG 1 cut(s) 73
AsuHPI GGTGA 2 cut(s) 203, 442
AvaII GGWCC 1 cut(s) 643
BaeGI GKGCMC 1 cut(s) 760
BamHI GGATCC 1 cut(s) 400
BanI GGYRCC 3 cut(s) 53, 75, 267
BarI GAAGNNNNNNTAC 2 cut(s) 52, 84
Bbv12I GWGCWC 3 cut(s) 552, 742, 760
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 5 cut(s) 103, 109, 327, 568, 738
BccI CCATC 2 cut(s) 296, 811
BciVI GTATCC 1 cut(s) 592
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 924
BcuI ACTAGT 2 cut(s) 47, 65
BfaI CTAG 2 cut(s) 48, 66
BfoI RGCGCY 1 cut(s) 57
BfuI GTATCC 1 cut(s) 592
BglII AGATCT 1 cut(s) 692
BisI GCNGC 7 cut(s) 92, 95, 98, 275, 316, 582, 727
BlpI GCTNAGC 1 cut(s) 577
BlsI GCNGC 7 cut(s) 93, 96, 99, 276, 317, 583, 728
BmcAI AGTACT 3 cut(s) 46, 64, 892
Bme1390I CCNGG 1 cut(s) 73
Bme18I GGWCC 1 cut(s) 643
BmgBI CACGTC 1 cut(s) 378
BmgT120I GGNCC 1 cut(s) 643
BmiI GGNNCC 4 cut(s) 55, 77, 269, 402
BmrFI CCNGG 1 cut(s) 73
BmrI ACTGGG 2 cut(s) 456, 794
BmsI GCATC 1 cut(s) 270
BmuI ACTGGG 2 cut(s) 456, 794
Bpu10I CCTNAGC 1 cut(s) 828
Bpu1102I GCTNAGC 1 cut(s) 577
BpuMI CCSGG 1 cut(s) 73
Bsa29I ATCGAT 2 cut(s) 201, 303
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 924
BsaJI CCNNGG 4 cut(s) 272, 525, 790, 864
BsaWI WCCGGW 1 cut(s) 442
Bsc4I CCNNNNNNNGG 1 cut(s) 869
Bse118I RCCGGY 1 cut(s) 955
Bse1I ACTGG 3 cut(s) 42, 462, 800
BseAI TCCGGA 1 cut(s) 442
BseCI ATCGAT 2 cut(s) 201, 303
BseDI CCNNGG 4 cut(s) 272, 525, 790, 864
BseGI GGATG 1 cut(s) 648
BseLI CCNNNNNNNGG 1 cut(s) 869
BseMII CTCAG 3 cut(s) 591, 831, 842
BseNI ACTGG 3 cut(s) 42, 462, 800
BseRI GAGGAG 1 cut(s) 556
BseSI GKGCMC 1 cut(s) 760
BseX3I CGGCCG 1 cut(s) 275
BseXI GCAGC 5 cut(s) 103, 109, 327, 568, 738
Bsh1236I CGCG 4 cut(s) 81, 274, 371, 395
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 3 cut(s) 277, 524, 959
BshNI GGYRCC 3 cut(s) 53, 75, 267
BshVI ATCGAT 2 cut(s) 201, 303
BsiEI CGRYCG 1 cut(s) 278
BsiHKAI GWGCWC 3 cut(s) 552, 742, 760
BsiSI CCGG 4 cut(s) 57, 72, 443, 956
BslI CCNNNNNNNGG 1 cut(s) 869
BsmAI GTCTC 1 cut(s) 924
BsnI GGCC 3 cut(s) 277, 524, 959
Bso31I GGTCTC 1 cut(s) 924
Bsp1286I GDGCHC 3 cut(s) 552, 742, 760
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 8 cut(s) 22, 85, 240, 307, 400, 624, 692, 820
Bsp1720I GCTNAGC 1 cut(s) 577
BspACI CCGC 7 cut(s) 79, 89, 95, 272, 274, 395, 729
BspANI GGCC 3 cut(s) 277, 524, 959
BspCNI CTCAG 3 cut(s) 590, 832, 841
BspDI ATCGAT 2 cut(s) 201, 303
BspEI TCCGGA 1 cut(s) 442
BspFNI CGCG 4 cut(s) 81, 274, 371, 395
BspLI GGNNCC 4 cut(s) 55, 77, 269, 402
BspPI GGATC 4 cut(s) 80, 395, 408, 828
BspT107I GGYRCC 3 cut(s) 53, 75, 267
BspTNI GGTCTC 1 cut(s) 924
BsrFI RCCGGY 1 cut(s) 955
BsrI ACTGG 3 cut(s) 42, 462, 800
BssAI RCCGGY 1 cut(s) 955
BssECI CCNNGG 4 cut(s) 272, 525, 790, 864
BssMI GATC 8 cut(s) 22, 85, 240, 307, 400, 624, 692, 820
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 2 cut(s) 790, 864
Bst4CI ACNGT 2 cut(s) 421, 872
Bst6I CTCTTC 2 cut(s) 177, 967
BstACI GRCGYC 1 cut(s) 54
BstC8I GCNNGC 2 cut(s) 393, 957
BstDEI CTNAG 5 cut(s) 577, 701, 717, 828, 840
BstDSI CCRYGG 1 cut(s) 272
BstEII GGTNACC 1 cut(s) 448
BstF5I GGATG 1 cut(s) 648
BstFNI CGCG 4 cut(s) 81, 274, 371, 395
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 2 cut(s) 56, 373
BstKTI GATC 8 cut(s) 25, 88, 243, 310, 403, 627, 695, 823
BstMAI GTCTC 1 cut(s) 924
BstMBI GATC 8 cut(s) 22, 85, 240, 307, 400, 624, 692, 820
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 3 cut(s) 97, 280, 360
BstNSI RCATGY 1 cut(s) 437
BstPI GGTNACC 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 71
BstSLI GKGCMC 1 cut(s) 760
BstUI CGCG 4 cut(s) 81, 274, 371, 395
BstV1I GCAGC 5 cut(s) 103, 109, 327, 568, 738
BstX2I RGATCY 2 cut(s) 400, 692
BstXI CCANNNNNNTGG 1 cut(s) 469
BstYI RGATCY 2 cut(s) 400, 692
BstZI CGGCCG 1 cut(s) 275
Bsu15I ATCGAT 2 cut(s) 201, 303
BsuI GTATCC 1 cut(s) 592
BsuRI GGCC 3 cut(s) 277, 524, 959
BsuTUI ATCGAT 2 cut(s) 201, 303
BtgI CCRYGG 1 cut(s) 272
BtgZI GCGATG 1 cut(s) 96
BtrI CACGTC 1 cut(s) 378
BtsCI GGATG 1 cut(s) 648
BtsIMutI CAGTG 1 cut(s) 807
Cac8I GCNNGC 2 cut(s) 393, 957
CfoI GCGC 2 cut(s) 56, 373
Cfr10I RCCGGY 1 cut(s) 955
Cfr13I GGNCC 1 cut(s) 643
Cfr42I CCGCGG 1 cut(s) 275
ClaI ATCGAT 2 cut(s) 201, 303
CseI GACGC 1 cut(s) 377
CviAII CATG 5 cut(s) 101, 139, 364, 434, 520
DdeI CTNAG 5 cut(s) 577, 701, 717, 828, 840
DinI GGCGCC 1 cut(s) 55
DpnI GATC 8 cut(s) 24, 87, 242, 309, 402, 626, 694, 822
DpnII GATC 8 cut(s) 22, 85, 240, 307, 400, 624, 692, 820
DraI TTTAAA 1 cut(s) 979
DraIII CACNNNGTG 1 cut(s) 397
DrdI GACNNNNNNGTC 1 cut(s) 844
DseDI GACNNNNNNGTC 1 cut(s) 844
EaeI YGGCCR 2 cut(s) 275, 522
EagI CGGCCG 1 cut(s) 275
Eam1104I CTCTTC 2 cut(s) 177, 967
EarI CTCTTC 2 cut(s) 177, 967
EclXI CGGCCG 1 cut(s) 275
Eco130I CCWWGG 2 cut(s) 790, 864
Eco31I GGTCTC 1 cut(s) 924
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 1 cut(s) 643
Eco52I CGGCCG 1 cut(s) 275
Eco91I GGTNACC 1 cut(s) 448
EcoO65I GGTNACC 1 cut(s) 448
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 2 cut(s) 790, 864
EgeI GGCGCC 1 cut(s) 55
EheI GGCGCC 1 cut(s) 55
ErhI CCWWGG 2 cut(s) 790, 864
FaeI CATG 5 cut(s) 104, 142, 367, 437, 523
FatI CATG 5 cut(s) 100, 138, 363, 433, 519
FauI CCCGC 2 cut(s) 279, 722
FauNDI CATATG 1 cut(s) 785
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 7 cut(s) 92, 95, 98, 275, 316, 582, 727
FokI GGATG 1 cut(s) 635
Fsp4HI GCNGC 7 cut(s) 92, 95, 98, 275, 316, 582, 727
FspBI CTAG 2 cut(s) 48, 66
GlaI GCGC 2 cut(s) 55, 372
GluI GCNGC 7 cut(s) 92, 95, 98, 275, 316, 582, 727
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 3 cut(s) 277, 524, 959
HapII CCGG 4 cut(s) 57, 72, 443, 956
HgaI GACGC 1 cut(s) 377
HhaI GCGC 2 cut(s) 56, 373
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 5 cut(s) 104, 142, 367, 437, 523
Hin6I GCGC 2 cut(s) 54, 371
HinP1I GCGC 2 cut(s) 54, 371
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HindIII AAGCTT 2 cut(s) 476, 671
HinfI GANTC 3 cut(s) 258, 356, 842
HpaII CCGG 4 cut(s) 57, 72, 443, 956
HphI GGTGA 2 cut(s) 203, 442
Hpy166II GTNNAC 3 cut(s) 414, 758, 778
Hpy188I TCNGA 4 cut(s) 172, 257, 629, 942
Hpy188III TCNNGA 2 cut(s) 26, 443
Hpy8I GTNNAC 3 cut(s) 414, 758, 778
Hpy99I CGWCG 4 cut(s) 119, 122, 850, 883
HpyAV CCTTC 6 cut(s) 182, 208, 343, 563, 624, 758
HpyCH4III ACNGT 2 cut(s) 421, 872
HpyCH4IV ACGT 1 cut(s) 377
HpyCH4V TGCA 4 cut(s) 584, 758, 919, 925
HpyF10VI GCNNNNNNNGC 3 cut(s) 97, 280, 360
HpyF3I CTNAG 5 cut(s) 577, 701, 717, 828, 840
HpySE526I ACGT 1 cut(s) 377
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 5 cut(s) 104, 142, 367, 437, 523
HspAI GCGC 2 cut(s) 54, 371
KasI GGCGCC 1 cut(s) 53
Kpn2I TCCGGA 1 cut(s) 442
KpnI GGTACC 2 cut(s) 79, 271
KroI GCCGGC 1 cut(s) 955
KroNI GCCGGC 1 cut(s) 957
Ksp22I TGATCA 1 cut(s) 307
KspI CCGCGG 1 cut(s) 275
Kzo9I GATC 8 cut(s) 22, 85, 240, 307, 400, 624, 692, 820
LmnI GCTCC 1 cut(s) 555
LpnPI CCDG 9 cut(s) 55, 70, 85, 373, 456, 475, 491, 813, 969
Lsp1109I GCAGC 5 cut(s) 103, 109, 327, 568, 738
LweI GCATC 1 cut(s) 270
MaeI CTAG 2 cut(s) 48, 66
MaeII ACGT 1 cut(s) 377
MaeIII GTNAC 3 cut(s) 448, 493, 802
MalI GATC 8 cut(s) 24, 87, 242, 309, 402, 626, 694, 822
MboI GATC 8 cut(s) 22, 85, 240, 307, 400, 624, 692, 820
MboII GAAGA 6 cut(s) 119, 194, 223, 308, 954, 961
MfeI CAATTG 1 cut(s) 920
MflI RGATCY 2 cut(s) 400, 692
MhlI GDGCHC 3 cut(s) 552, 742, 760
MluCI AATT 2 cut(s) 130, 920
Mly113I GGCGCC 1 cut(s) 54
MlyI GAGTC 2 cut(s) 365, 851
MroI TCCGGA 1 cut(s) 442
MroNI GCCGGC 1 cut(s) 955
MseI TTAA 5 cut(s) 225, 386, 591, 978, 991
MspA1I CMGCKG 3 cut(s) 274, 581, 729
MspI CCGG 4 cut(s) 57, 72, 443, 956
MspR9I CCNGG 1 cut(s) 73
MunI CAATTG 1 cut(s) 920
MvnI CGCG 4 cut(s) 81, 274, 371, 395
MwoI GCNNNNNNNGC 3 cut(s) 97, 280, 360
NaeI GCCGGC 1 cut(s) 957
NarI GGCGCC 1 cut(s) 54
NciI CCSGG 1 cut(s) 73
NdeI CATATG 1 cut(s) 785
NdeII GATC 8 cut(s) 22, 85, 240, 307, 400, 624, 692, 820
NgoMIV GCCGGC 1 cut(s) 955
NlaIII CATG 5 cut(s) 104, 142, 367, 437, 523
NlaIV GGNNCC 4 cut(s) 55, 77, 269, 402
NmeAIII GCCGAG 1 cut(s) 550
NmuCI GTSAC 3 cut(s) 448, 493, 802
NspI RCATGY 1 cut(s) 437
PciI ACATGT 1 cut(s) 433
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PdiI GCCGGC 1 cut(s) 957
PfeI GAWTC 1 cut(s) 258
PflFI GACNNNGTC 2 cut(s) 379, 841
PkrI GCNGC 7 cut(s) 93, 96, 99, 276, 317, 583, 728
PleI GAGTC 2 cut(s) 364, 850
PluTI GGCGCC 1 cut(s) 57
PpsI GAGTC 2 cut(s) 364, 850
PscI ACATGT 1 cut(s) 433
PspEI GGTNACC 1 cut(s) 448
PspN4I GGNNCC 4 cut(s) 55, 77, 269, 402
PspPI GGNCC 1 cut(s) 643
PsuI RGATCY 2 cut(s) 400, 692
PsyI GACNNNGTC 2 cut(s) 379, 841
PvuII CAGCTG 1 cut(s) 581
SacII CCGCGG 1 cut(s) 275
SaqAI TTAA 5 cut(s) 225, 386, 591, 978, 991
SatI GCNGC 7 cut(s) 92, 95, 98, 275, 316, 582, 727
Sau3AI GATC 8 cut(s) 22, 85, 240, 307, 400, 624, 692, 820
Sau96I GGNCC 1 cut(s) 643
ScaI AGTACT 3 cut(s) 46, 64, 892
SchI GAGTC 2 cut(s) 365, 851
ScrFI CCNGG 1 cut(s) 73
SduI GDGCHC 3 cut(s) 552, 742, 760
SfaNI GCATC 1 cut(s) 270
SfoI GGCGCC 1 cut(s) 55
Sfr303I CCGCGG 1 cut(s) 275
SgrBI CCGCGG 1 cut(s) 275
SinI GGWCC 1 cut(s) 643
SpeI ACTAGT 2 cut(s) 47, 65
Sse9I AATT 2 cut(s) 130, 920
SsiI CCGC 7 cut(s) 79, 89, 95, 272, 274, 395, 729
SspDI GGCGCC 1 cut(s) 53
SspMI CTAG 2 cut(s) 48, 66
StyD4I CCNGG 1 cut(s) 71
StyI CCWWGG 2 cut(s) 790, 864
TaaI ACNGT 2 cut(s) 421, 872
TaiI ACGT 1 cut(s) 380
TaqI TCGA 2 cut(s) 201, 303
TasI AATT 2 cut(s) 130, 920
TatI WGTACW 5 cut(s) 44, 62, 123, 144, 890
TauI GCSGC 2 cut(s) 97, 277
TfiI GAWTC 1 cut(s) 258
Tru1I TTAA 5 cut(s) 225, 386, 591, 978, 991
Tru9I TTAA 5 cut(s) 225, 386, 591, 978, 991
TscAI CASTG 1 cut(s) 807
TseFI GTSAC 3 cut(s) 448, 493, 802
TseI GCWGC 5 cut(s) 91, 97, 315, 581, 726
Tsp45I GTSAC 3 cut(s) 448, 493, 802
TspDTI ATGAA 4 cut(s) 120, 155, 553, 954
TspGWI ACGGA 1 cut(s) 393
TspRI CASTG 1 cut(s) 807
Tth111I GACNNNGTC 2 cut(s) 379, 841
VneI GTGCAC 1 cut(s) 756
VpaK11BI GGWCC 1 cut(s) 643
XceI RCATGY 1 cut(s) 437
XspI CTAG 2 cut(s) 48, 66
ZrmI AGTACT 3 cut(s) 46, 64, 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.