Rroxscaffold_1G00075760

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012091
Physical Location & Seq
Forward (+)
96379709 .. 96388153
8445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075760.1

Sequence Viewer

Length: 1086 bp
ATGTCAGTGTGGTTGACGACGAAGGAGACCGGGGCGGCCTTGCCCTTGCACTCAGAAATAGATCATGATGAAATTTATCGGACGAGGAGCACTACTGGCCATGAAGGAAGTGAATACCGCCAGCCGCAGCAACAGTACCACCGTGATGAAGATGAGTACCAGCTGCAAGGCCAGTTTCAGCACGAGTGCAATATCTCAAGGAATAGTGTATGGGAAATAGAAGAGGAAGAAGAAGAAGAAGAAGATGATCATGACGGTTTGAATTATTCAATTGGAGGATCAAATACTCGTATCCCAATTGAGATCAAAGAGCTTTTCGGGGCGAAGCGCTACTTTCCCATGCCTTCCATCAAGGAAGAACAATATGATCATATTAATGCTCTGCCTCATTCTTCTTCATCGTCTGACATTGTTGACTGCGTTTATGTGGCCGTCGGAAAGAGCGAGTCAAGCATGGACGCCTTAAACTACGCCGTCAAGCAGCTGCTGCTGTCATCCAACACTATCCTCTATCTCATACACATTTTCCCAGAGATCAAGTACATCCCAAGTCCATTAGGAAAACTTCCAAAAAGTCAAGTGAGCCCAGAACAAGTGGAAAGTTTCATAGCTCAAGAATCAGACAAGAGAAGACAGCTCCTTCAGAAGTATATTGATAAATGCTCGGCTGCAAAGGTTAAGATGGACACTCTGCTGATTGAAAGTGATATGATCTCCAAGGCTATTGTGGACCTTATCCCTATTCTCAACATAAGAAAACTGGTGCTTGGAACGAACAAATCTAATTTGAGGAAATTGATGTCCGGGAAAGGAGCAAGGGGAATAGCTGACCAAGTCCTTCAAAGTGCACCCCAAGGTTGTGAGATTAATATCATTTGTGAAGGAAATCAAGTGATGATTAATGAGTCGCTTTCCCCACATGGCATTGCCAATGATCACATTGCACCAAATACACTTTCCCCTAGAGGAGGAGGCAATGATGATAACCCTAACTCTATCCTAGACCGCGATCAGAGGAATGAAGCCTGCGATCGGAGGAATGAAGCCTGTTCATTCCTGTGTTTTAAACCCATGTTCTCCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

361

Amino Acids

40.7

Weight (kDa)

5.07

Isoelectric Point (pI)

69.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012091)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47740 AT5G47740
fragaria_vesca FvH4_3g00270 FvH4_3g00270 FvH4_3g00270
malus_domestica MD05G1362400.v1.1
prunus_persica Prupe.4G003000_v2.0.a1
pyrus_communis pycom05g32880
rosa_chinensis RchiOBHm_Chr5g0000401
rosa_laevigata RLG00000030863
rosa_multiflora Rmu_sc0017532.1_g000001 Rmu_ssc0000181.1_g000001
rosa_roxburghii Rroxscaffold_1G00075760
rosa_rugosa Rorug04G0383300
rosa_samantha Rh5AG003100 Rh5BG004300 Rh5CG003300 Rh5DG003300
rosa_wichuraiana Rw5G000320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1008
AciI CCGC 4 cut(s) 35, 118, 125, 1006
AclWI GGATC 1 cut(s) 286
AcoI YGGCCR 2 cut(s) 97, 429
AcsI RAATTY 1 cut(s) 72
AcuI CTGAAG 1 cut(s) 626
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 3 cut(s) 137, 158, 542
AfeI AGCGCT 1 cut(s) 329
AfiI CCNNNNNNNGG 2 cut(s) 968, 1032
AgsI TTSAA 4 cut(s) 262, 270, 701, 842
AjuI GAANNNNNNNTTGG 2 cut(s) 940, 972
AluBI AGCT 6 cut(s) 163, 313, 484, 611, 637, 827
AluI AGCT 6 cut(s) 163, 313, 484, 611, 637, 827
Alw21I GWGCWC 2 cut(s) 92, 850
Alw26I GTCTC 1 cut(s) 20
Alw44I GTGCAC 1 cut(s) 846
AlwI GGATC 1 cut(s) 286
AlwNI CAGNNNCTG 1 cut(s) 487
Aor51HI AGCGCT 1 cut(s) 329
AoxI GGCC 4 cut(s) 36, 97, 169, 429
ApaLI GTGCAC 1 cut(s) 846
ApeKI GCWGC 6 cut(s) 127, 163, 481, 484, 487, 668
ApoI RAATTY 1 cut(s) 72
AseI ATTAAT 3 cut(s) 375, 867, 900
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 730
AsuC2I CCSGG 2 cut(s) 31, 805
AvaII GGWCC 1 cut(s) 730
BaeGI GKGCMC 1 cut(s) 850
BalI TGGCCA 1 cut(s) 99
BanII GRGCYC 1 cut(s) 587
BauI CACGAG 1 cut(s) 182
BbsI GAAGAC 1 cut(s) 637
Bbv12I GWGCWC 2 cut(s) 92, 850
BbvI GCAGC 6 cut(s) 139, 150, 471, 474, 493, 655
BccI CCATC 2 cut(s) 356, 676
BceAI ACGGC 2 cut(s) 416, 458
BciVI GTATCC 1 cut(s) 302
BclI TGATCA 3 cut(s) 247, 367, 934
BcnI CCSGG 2 cut(s) 31, 805
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 2 cut(s) 963, 1001
BfoI RGCGCY 1 cut(s) 331
BfuI GTATCC 1 cut(s) 302
BisI GCNGC 8 cut(s) 36, 125, 128, 164, 482, 485, 488, 669
BlsI GCNGC 8 cut(s) 37, 126, 129, 165, 483, 486, 489, 670
Bme1390I CCNGG 2 cut(s) 31, 805
Bme18I GGWCC 1 cut(s) 730
BmgT120I GGNCC 1 cut(s) 730
BmrFI CCNGG 2 cut(s) 31, 805
BpiI GAAGAC 1 cut(s) 637
BpuEI CTTGAG 2 cut(s) 181, 597
BpuMI CCSGG 2 cut(s) 31, 805
BsaBI GATNNNNATC 1 cut(s) 869
BsaHI GRCGYC 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 3 cut(s) 30, 717, 853
Bsc4I CCNNNNNNNGG 2 cut(s) 968, 1032
Bse1I ACTGG 3 cut(s) 100, 172, 765
Bse3DI GCAATG 3 cut(s) 924, 939, 982
Bse8I GATNNNNATC 1 cut(s) 869
BseDI CCNNGG 3 cut(s) 30, 717, 853
BseGI GGATG 2 cut(s) 494, 543
BseJI GATNNNNATC 1 cut(s) 869
BseLI CCNNNNNNNGG 2 cut(s) 968, 1032
BseMI GCAATG 3 cut(s) 924, 939, 982
BseMII CTCAG 1 cut(s) 66
BseNI ACTGG 3 cut(s) 100, 172, 765
BseRI GAGGAG 3 cut(s) 100, 981, 984
BseSI GKGCMC 1 cut(s) 850
BseXI GCAGC 6 cut(s) 139, 150, 471, 474, 493, 655
Bsh1236I CGCG 1 cut(s) 1008
Bsh1285I CGRYCG 1 cut(s) 1033
BshFI GGCC 4 cut(s) 38, 99, 171, 431
BsiEI CGRYCG 1 cut(s) 1033
BsiHKAI GWGCWC 2 cut(s) 92, 850
BsiSI CCGG 2 cut(s) 30, 804
BslI CCNNNNNNNGG 2 cut(s) 968, 1032
BsmAI GTCTC 1 cut(s) 20
BsnI GGCC 4 cut(s) 38, 99, 171, 431
Bso31I GGTCTC 1 cut(s) 20
Bsp1286I GDGCHC 3 cut(s) 92, 587, 850
BspACI CCGC 4 cut(s) 35, 118, 125, 1006
BspANI GGCC 4 cut(s) 38, 99, 171, 431
BspCNI CTCAG 1 cut(s) 65
BspFNI CGCG 1 cut(s) 1008
BspHI TCATGA 2 cut(s) 64, 250
BspPI GGATC 1 cut(s) 286
BspTNI GGTCTC 1 cut(s) 20
BsrDI GCAATG 3 cut(s) 924, 939, 982
BsrI ACTGG 3 cut(s) 100, 172, 765
BssECI CCNNGG 3 cut(s) 30, 717, 853
BssNI GRCGYC 1 cut(s) 459
BssSI CACGAG 1 cut(s) 182
BssT1I CCWWGG 2 cut(s) 717, 853
Bst2BI CACGAG 1 cut(s) 182
Bst4CI ACNGT 3 cut(s) 135, 143, 257
Bst6I CTCTTC 1 cut(s) 216
BstACI GRCGYC 1 cut(s) 459
BstAPI GCANNNNNTGC 1 cut(s) 487
BstC8I GCNNGC 2 cut(s) 122, 1027
BstDEI CTNAG 1 cut(s) 52
BstENI CCTNNNNNAGG 1 cut(s) 966
BstF5I GGATG 2 cut(s) 494, 543
BstFNI CGCG 1 cut(s) 1008
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 1 cut(s) 330
BstMAI GTCTC 1 cut(s) 20
BstMCI CGRYCG 1 cut(s) 1033
BstMWI GCNNNNNNNGC 3 cut(s) 96, 450, 487
BstSCI CCNGG 2 cut(s) 29, 803
BstSLI GKGCMC 1 cut(s) 850
BstUI CGCG 1 cut(s) 1008
BstV1I GCAGC 6 cut(s) 139, 150, 471, 474, 493, 655
BstV2I GAAGAC 1 cut(s) 637
BsuI GTATCC 1 cut(s) 302
BsuRI GGCC 4 cut(s) 38, 99, 171, 431
BtsCI GGATG 2 cut(s) 494, 543
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 2 cut(s) 122, 1027
CaiI CAGNNNCTG 1 cut(s) 487
CciI TCATGA 2 cut(s) 64, 250
CfoI GCGC 1 cut(s) 330
Cfr13I GGNCC 1 cut(s) 730
CseI GACGC 1 cut(s) 467
Csp6I GTAC 3 cut(s) 136, 157, 541
CviAII CATG 7 cut(s) 65, 101, 251, 340, 454, 920, 1072
CviQI GTAC 3 cut(s) 136, 157, 541
DdeI CTNAG 1 cut(s) 52
DraI TTTAAA 1 cut(s) 1066
EaeI YGGCCR 2 cut(s) 97, 429
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 717, 853
Eco24I GRGCYC 1 cut(s) 587
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 730
Eco47III AGCGCT 1 cut(s) 329
Eco57I CTGAAG 1 cut(s) 626
EcoNI CCTNNNNNAGG 1 cut(s) 966
EcoT14I CCWWGG 2 cut(s) 717, 853
EcoT38I GRGCYC 1 cut(s) 587
ErhI CCWWGG 2 cut(s) 717, 853
FaeI CATG 7 cut(s) 68, 104, 254, 343, 457, 923, 1075
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FatI CATG 7 cut(s) 64, 100, 250, 339, 453, 919, 1071
FbaI TGATCA 3 cut(s) 247, 367, 934
Fnu4HI GCNGC 8 cut(s) 36, 125, 128, 164, 482, 485, 488, 669
FokI GGATG 2 cut(s) 481, 530
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 8 cut(s) 36, 125, 128, 164, 482, 485, 488, 669
FspBI CTAG 2 cut(s) 963, 1001
GlaI GCGC 1 cut(s) 329
GluI GCNGC 8 cut(s) 36, 125, 128, 164, 482, 485, 488, 669
HaeII RGCGCY 1 cut(s) 331
HaeIII GGCC 4 cut(s) 38, 99, 171, 431
HapII CCGG 2 cut(s) 30, 804
HgaI GACGC 1 cut(s) 467
HhaI GCGC 1 cut(s) 330
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 7 cut(s) 68, 104, 254, 343, 457, 923, 1075
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HincII GTYRAC 2 cut(s) 15, 415
HindII GTYRAC 2 cut(s) 15, 415
HinfI GANTC 3 cut(s) 446, 617, 905
HpaII CCGG 2 cut(s) 30, 804
Hpy166II GTNNAC 4 cut(s) 15, 415, 730, 848
Hpy188I TCNGA 8 cut(s) 55, 81, 406, 437, 622, 645, 1014, 1035
Hpy188III TCNNGA 3 cut(s) 65, 251, 614
Hpy8I GTNNAC 4 cut(s) 15, 415, 730, 848
Hpy99I CGWCG 2 cut(s) 22, 437
HpyAV CCTTC 6 cut(s) 16, 98, 354, 650, 848, 875
HpyCH4III ACNGT 3 cut(s) 135, 143, 257
HpyCH4V TGCA 6 cut(s) 49, 166, 189, 671, 848, 944
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 450, 487
HpyF3I CTNAG 1 cut(s) 52
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 7 cut(s) 68, 104, 254, 343, 457, 923, 1075
HspAI GCGC 1 cut(s) 328
Ksp22I TGATCA 3 cut(s) 247, 367, 934
LmnI GCTCC 3 cut(s) 87, 642, 812
Lsp1109I GCAGC 6 cut(s) 139, 150, 471, 474, 493, 655
MaeI CTAG 2 cut(s) 963, 1001
MfeI CAATTG 2 cut(s) 270, 297
MhlI GDGCHC 3 cut(s) 92, 587, 850
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 6 cut(s) 72, 262, 270, 297, 784, 794
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 2 cut(s) 455, 914
MmeI TCCRAC 2 cut(s) 415, 522
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MseI TTAA 6 cut(s) 375, 464, 678, 867, 900, 1065
MslI CAYNNNNRTG 3 cut(s) 144, 375, 1057
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 2 cut(s) 163, 484
MspI CCGG 2 cut(s) 30, 804
MspR9I CCNGG 2 cut(s) 31, 805
MunI CAATTG 2 cut(s) 270, 297
MvnI CGCG 1 cut(s) 1008
MwoI GCNNNNNNNGC 3 cut(s) 96, 450, 487
NciI CCSGG 2 cut(s) 31, 805
NlaIII CATG 7 cut(s) 68, 104, 254, 343, 457, 923, 1075
NmeAIII GCCGAG 1 cut(s) 644
PagI TCATGA 2 cut(s) 64, 250
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PfeI GAWTC 1 cut(s) 617
PflFI GACNNNGTC 1 cut(s) 833
PfoI TCCNGGA 1 cut(s) 803
PkrI GCNGC 8 cut(s) 37, 126, 129, 165, 483, 486, 489, 670
Ple19I CGATCG 1 cut(s) 1033
PleI GAGTC 2 cut(s) 454, 913
PpsI GAGTC 2 cut(s) 454, 913
PshBI ATTAAT 3 cut(s) 375, 867, 900
PspPI GGNCC 1 cut(s) 730
PstNI CAGNNNCTG 1 cut(s) 487
PsyI GACNNNGTC 1 cut(s) 833
PvuI CGATCG 1 cut(s) 1033
PvuII CAGCTG 2 cut(s) 163, 484
RsaI GTAC 3 cut(s) 137, 158, 542
RsaNI GTAC 3 cut(s) 136, 157, 541
RseI CAYNNNNRTG 3 cut(s) 144, 375, 1057
SaqAI TTAA 6 cut(s) 375, 464, 678, 867, 900, 1065
SatI GCNGC 8 cut(s) 36, 125, 128, 164, 482, 485, 488, 669
Sau96I GGNCC 1 cut(s) 730
SchI GAGTC 2 cut(s) 455, 914
ScrFI CCNGG 2 cut(s) 31, 805
SduI GDGCHC 3 cut(s) 92, 587, 850
SetI ASST 9 cut(s) 165, 315, 486, 613, 639, 678, 735, 829, 859
SinI GGWCC 1 cut(s) 730
SmiMI CAYNNNNRTG 3 cut(s) 144, 375, 1057
SmlI CTYRAG 2 cut(s) 196, 612
SmoI CTYRAG 2 cut(s) 196, 612
Sse9I AATT 6 cut(s) 72, 262, 270, 297, 784, 794
SsiI CCGC 4 cut(s) 35, 118, 125, 1006
SspMI CTAG 2 cut(s) 963, 1001
StyD4I CCNGG 2 cut(s) 29, 803
StyI CCWWGG 2 cut(s) 717, 853
TaaI ACNGT 3 cut(s) 135, 143, 257
TasI AATT 6 cut(s) 72, 262, 270, 297, 784, 794
TatI WGTACW 1 cut(s) 540
TauI GCSGC 2 cut(s) 38, 127
TfiI GAWTC 1 cut(s) 617
Tru1I TTAA 6 cut(s) 375, 464, 678, 867, 900, 1065
Tru9I TTAA 6 cut(s) 375, 464, 678, 867, 900, 1065
TscAI CASTG 1 cut(s) 12
TseI GCWGC 6 cut(s) 127, 163, 481, 484, 487, 668
TspDTI ATGAA 8 cut(s) 84, 117, 162, 387, 595, 1035, 1041, 1056
TspRI CASTG 1 cut(s) 12
Tth111I GACNNNGTC 1 cut(s) 833
VneI GTGCAC 1 cut(s) 846
VpaK11BI GGWCC 1 cut(s) 730
VspI ATTAAT 3 cut(s) 375, 867, 900
XagI CCTNNNNNAGG 1 cut(s) 966
XapI RAATTY 1 cut(s) 72
XcmI CCANNNNNNNNNTGG 1 cut(s) 724
XspI CTAG 2 cut(s) 963, 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.