Rw5G000320

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012091
Physical Location & Seq
Reverse (-)
288813 .. 291904
3092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G000320.1

Sequence Viewer

Length: 1164 bp
ATGTCACCATCTACCAACCCCACACCAATCATCATTAAGGGAACCGTGGTCGACGGTGGTGCCGACTTGCCCATCTCTCATCGGATCGACGGTTCTCGACTGGCACAGCTAGCACGTGTGGTTGACGACGAAGGAGACCGGGGCGGCCTTGCCCTTGCACTCAGAATAGATCATGATGAAATTCGTCGGACGAGGAACACCACTGGCCATGAAGGAAGCGAATACCGTCAGCCGCAGCAACAGTACCACCGTGATGAAGATGAGTACCAGCTGCAAGGCCAGTTTCAGCACGAGTGCAATATCTCAAGGAATAGTGTATGGGAGATAGAAGAGGAAGAAGAAGAAGATGATCATGAGGGTTTGAATTATTCAATTGGAGGATCAAATACTAGTATCCCAATTGAGATCAAAGAGCTTTTCGGGGCGAAGCGCTACTTTCCCATGCCTTCCATCAAGGAAGAACAATATGATATTAATGGTCTGCCTCATTCTTCTTCATCGTCTGACATTGTTGACTGCGTTTATGTGGCCGTCGGAAAGAGCGAGTCAAGCATGGACGCCTTAAACTACGCCGTCAAGCAGCTGCTGCTGTCATCCAACACTATCCTCTATCTCATACACATTTTCCCTGAGATCAAGTACATCCCAAGTCCATTAGGAAAACTCCCAAAAAGTCAAGTGAGCCCAGACCAAGTGGAAAGTTTCATGGCCCAAGAATCAGACAAGAGAAGACAGCTCCTTCAGAAGTATATTGATAAATGCTCGGCTGCAAAGGTTAAGATGGATACTCTGCTGATTGAAAGTGATATGATCGCCAAGGCTATTGTGGACCTTATCCCTATTCTCAACATCAGAAAACTGGTGCTTGGAACGAACAAATCTAATTTGAGGAAATTGATGTCCGGGAAGGGAGCAAGGGGAATAGCTGATCAAGTCCTTCAAAGTGCACCCCAAGGTTGTGAGATTAATATCATCTGTGAAGGAAATCAAGTGATGACTAATGACTCGCTTTCCCCACGTGGCGTTGCCAATGATCGCATTGCACCAAATACACTTTCCCCTAGAGGAGGTGGCAATGATGACAACCCTAACTCTATCCTAGACCGCGATCAGAGGAATGAAGCCTGTTCATTCCTGTGTTTTAAACCCATGTTCTCCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Usp

Protein Analysis

387

Amino Acids

43.02

Weight (kDa)

5.12

Isoelectric Point (pI)

56.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012091)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47740 AT5G47740
fragaria_vesca FvH4_3g00270 FvH4_3g00270 FvH4_3g00270
malus_domestica MD05G1362400.v1.1
prunus_persica Prupe.4G003000_v2.0.a1
pyrus_communis pycom05g32880
rosa_chinensis RchiOBHm_Chr5g0000401
rosa_laevigata RLG00000030863
rosa_multiflora Rmu_sc0017532.1_g000001 Rmu_ssc0000181.1_g000001
rosa_roxburghii Rroxscaffold_1G00075760
rosa_rugosa Rorug04G0383300
rosa_samantha Rh5AG003100 Rh5BG004300 Rh5CG003300 Rh5DG003300
rosa_wichuraiana Rw5G000320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 51
AccII CGCG 1 cut(s) 1107
AciI CCGC 3 cut(s) 144, 233, 1105
AclWI GGATC 2 cut(s) 92, 388
AcoI YGGCCR 2 cut(s) 205, 528
AcsI RAATTY 1 cut(s) 180
AcuI CTGAAG 1 cut(s) 725
AcvI CACGTG 2 cut(s) 116, 1019
AcyI GRCGYC 1 cut(s) 558
AfaI GTAC 3 cut(s) 245, 266, 641
AfeI AGCGCT 1 cut(s) 431
AfiI CCNNNNNNNGG 1 cut(s) 1067
AflIII ACRYGT 1 cut(s) 115
AgsI TTSAA 4 cut(s) 364, 372, 800, 941
AhlI ACTAGT 1 cut(s) 389
AjuI GAANNNNNNNTTGG 2 cut(s) 1039, 1071
AloI GAACNNNNNNTCC 2 cut(s) 76, 108
AluBI AGCT 6 cut(s) 109, 271, 415, 583, 736, 926
AluI AGCT 6 cut(s) 109, 271, 415, 583, 736, 926
Alw21I GWGCWC 1 cut(s) 949
Alw26I GTCTC 1 cut(s) 129
Alw44I GTGCAC 1 cut(s) 945
AlwI GGATC 2 cut(s) 92, 388
AlwNI CAGNNNCTG 1 cut(s) 586
Aor51HI AGCGCT 1 cut(s) 431
AoxI GGCC 5 cut(s) 145, 205, 277, 528, 708
ApaLI GTGCAC 1 cut(s) 945
ApeKI GCWGC 6 cut(s) 235, 271, 580, 583, 586, 767
ApoI RAATTY 1 cut(s) 180
AseI ATTAAT 2 cut(s) 474, 966
AspLEI GCGC 1 cut(s) 432
AspS9I GGNCC 2 cut(s) 709, 829
AsuC2I CCSGG 2 cut(s) 140, 904
AsuNHI GCTAGC 1 cut(s) 109
AvaII GGWCC 1 cut(s) 829
BaeGI GKGCMC 1 cut(s) 949
BalI TGGCCA 1 cut(s) 207
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 686
BauI CACGAG 1 cut(s) 290
BbrPI CACGTG 2 cut(s) 116, 1019
BbsI GAAGAC 1 cut(s) 736
Bbv12I GWGCWC 1 cut(s) 949
BbvI GCAGC 6 cut(s) 247, 258, 570, 573, 592, 754
BccI CCATC 4 cut(s) 16, 80, 458, 775
BceAI ACGGC 2 cut(s) 515, 557
BcgI CGANNNNNNTGC 2 cut(s) 41, 75
BciVI GTATCC 2 cut(s) 404, 778
BclI TGATCA 2 cut(s) 349, 928
BcnI CCSGG 2 cut(s) 140, 904
BcoDI GTCTC 1 cut(s) 129
BcuI ACTAGT 1 cut(s) 389
BfaI CTAG 4 cut(s) 110, 390, 1062, 1100
BfoI RGCGCY 1 cut(s) 433
BfuI GTATCC 2 cut(s) 404, 778
BisI GCNGC 8 cut(s) 145, 233, 236, 272, 581, 584, 587, 768
BlsI GCNGC 8 cut(s) 146, 234, 237, 273, 582, 585, 588, 769
Bme1390I CCNGG 2 cut(s) 140, 904
Bme18I GGWCC 1 cut(s) 829
BmgT120I GGNCC 2 cut(s) 709, 829
BmiI GGNNCC 2 cut(s) 43, 61
BmrFI CCNGG 2 cut(s) 140, 904
BmtI GCTAGC 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 736
BpuEI CTTGAG 1 cut(s) 289
BpuMI CCSGG 2 cut(s) 140, 904
BsaAI YACGTR 2 cut(s) 116, 1019
BsaBI GATNNNNATC 1 cut(s) 968
BsaHI GRCGYC 1 cut(s) 558
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 4 cut(s) 45, 139, 816, 952
Bsc4I CCNNNNNNNGG 1 cut(s) 1067
Bse1I ACTGG 4 cut(s) 105, 208, 280, 864
Bse3DI GCAATG 2 cut(s) 1038, 1081
Bse8I GATNNNNATC 1 cut(s) 968
BseDI CCNNGG 4 cut(s) 45, 139, 816, 952
BseGI GGATG 2 cut(s) 593, 642
BseJI GATNNNNATC 1 cut(s) 968
BseLI CCNNNNNNNGG 1 cut(s) 1067
BseMI GCAATG 2 cut(s) 1038, 1081
BseMII CTCAG 2 cut(s) 175, 621
BseNI ACTGG 4 cut(s) 105, 208, 280, 864
BseRI GAGGAG 1 cut(s) 1080
BseSI GKGCMC 1 cut(s) 949
BseXI GCAGC 6 cut(s) 247, 258, 570, 573, 592, 754
Bsh1236I CGCG 1 cut(s) 1107
BshFI GGCC 5 cut(s) 147, 207, 279, 530, 710
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 949
BsiSI CCGG 2 cut(s) 139, 903
BslI CCNNNNNNNGG 1 cut(s) 1067
BsmAI GTCTC 1 cut(s) 129
BsnI GGCC 5 cut(s) 147, 207, 279, 530, 710
Bso31I GGTCTC 1 cut(s) 129
Bsp1286I GDGCHC 2 cut(s) 686, 949
BspACI CCGC 3 cut(s) 144, 233, 1105
BspANI GGCC 5 cut(s) 147, 207, 279, 530, 710
BspCNI CTCAG 2 cut(s) 174, 622
BspFNI CGCG 1 cut(s) 1107
BspHI TCATGA 2 cut(s) 172, 352
BspLI GGNNCC 2 cut(s) 43, 61
BspOI GCTAGC 1 cut(s) 113
BspPI GGATC 2 cut(s) 92, 388
BspT107I GGYRCC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 129
BsrDI GCAATG 2 cut(s) 1038, 1081
BsrI ACTGG 4 cut(s) 105, 208, 280, 864
BssECI CCNNGG 4 cut(s) 45, 139, 816, 952
BssNI GRCGYC 1 cut(s) 558
BssSI CACGAG 1 cut(s) 290
BssT1I CCWWGG 2 cut(s) 816, 952
Bst2BI CACGAG 1 cut(s) 290
Bst4CI ACNGT 6 cut(s) 46, 56, 92, 227, 243, 251
Bst6I CTCTTC 1 cut(s) 324
BstACI GRCGYC 1 cut(s) 558
BstAPI GCANNNNNTGC 1 cut(s) 586
BstBAI YACGTR 2 cut(s) 116, 1019
BstC8I GCNNGC 1 cut(s) 111
BstDEI CTNAG 2 cut(s) 161, 630
BstDSI CCRYGG 1 cut(s) 45
BstENI CCTNNNNNAGG 1 cut(s) 1065
BstF5I GGATG 2 cut(s) 593, 642
BstFNI CGCG 1 cut(s) 1107
BstH2I RGCGCY 1 cut(s) 433
BstHHI GCGC 1 cut(s) 432
BstMAI GTCTC 1 cut(s) 129
BstMWI GCNNNNNNNGC 3 cut(s) 110, 549, 586
BstSCI CCNGG 2 cut(s) 138, 902
BstSLI GKGCMC 1 cut(s) 949
BstUI CGCG 1 cut(s) 1107
BstV1I GCAGC 6 cut(s) 247, 258, 570, 573, 592, 754
BstV2I GAAGAC 1 cut(s) 736
BsuI GTATCC 2 cut(s) 404, 778
BsuRI GGCC 5 cut(s) 147, 207, 279, 530, 710
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 2 cut(s) 593, 642
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 111
CaiI CAGNNNCTG 1 cut(s) 586
CciI TCATGA 2 cut(s) 172, 352
CfoI GCGC 1 cut(s) 432
Cfr13I GGNCC 2 cut(s) 709, 829
CseI GACGC 1 cut(s) 566
Csp6I GTAC 3 cut(s) 244, 265, 640
CviAII CATG 7 cut(s) 173, 209, 353, 442, 553, 706, 1150
CviQI GTAC 3 cut(s) 244, 265, 640
DdeI CTNAG 2 cut(s) 161, 630
DraI TTTAAA 1 cut(s) 1144
EaeI YGGCCR 2 cut(s) 205, 528
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco130I CCWWGG 2 cut(s) 816, 952
Eco24I GRGCYC 1 cut(s) 686
Eco31I GGTCTC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 829
Eco47III AGCGCT 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 725
Eco72I CACGTG 2 cut(s) 116, 1019
EcoNI CCTNNNNNAGG 1 cut(s) 1065
EcoT14I CCWWGG 2 cut(s) 816, 952
EcoT38I GRGCYC 1 cut(s) 686
ErhI CCWWGG 2 cut(s) 816, 952
FaeI CATG 7 cut(s) 176, 212, 356, 445, 556, 709, 1153
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FatI CATG 7 cut(s) 172, 208, 352, 441, 552, 705, 1149
FbaI TGATCA 2 cut(s) 349, 928
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 8 cut(s) 145, 233, 236, 272, 581, 584, 587, 768
FokI GGATG 2 cut(s) 580, 629
FriOI GRGCYC 1 cut(s) 686
Fsp4HI GCNGC 8 cut(s) 145, 233, 236, 272, 581, 584, 587, 768
FspBI CTAG 4 cut(s) 110, 390, 1062, 1100
GlaI GCGC 1 cut(s) 431
GluI GCNGC 8 cut(s) 145, 233, 236, 272, 581, 584, 587, 768
HaeII RGCGCY 1 cut(s) 433
HaeIII GGCC 5 cut(s) 147, 207, 279, 530, 710
HapII CCGG 2 cut(s) 139, 903
HgaI GACGC 1 cut(s) 566
HhaI GCGC 1 cut(s) 432
Hin1I GRCGYC 1 cut(s) 558
Hin1II CATG 7 cut(s) 176, 212, 356, 445, 556, 709, 1153
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HincII GTYRAC 3 cut(s) 52, 124, 514
HindII GTYRAC 3 cut(s) 52, 124, 514
HinfI GANTC 3 cut(s) 545, 716, 1004
HpaII CCGG 2 cut(s) 139, 903
Hpy166II GTNNAC 5 cut(s) 52, 124, 514, 829, 947
Hpy188I TCNGA 9 cut(s) 84, 164, 189, 505, 536, 721, 744, 854, 1113
Hpy188III TCNNGA 3 cut(s) 96, 173, 353
Hpy8I GTNNAC 5 cut(s) 52, 124, 514, 829, 947
Hpy99I CGWCG 5 cut(s) 56, 92, 131, 189, 536
HpyAV CCTTC 7 cut(s) 125, 206, 456, 749, 901, 947, 974
HpyCH4III ACNGT 6 cut(s) 46, 56, 92, 227, 243, 251
HpyCH4IV ACGT 2 cut(s) 115, 1018
HpyCH4V TGCA 6 cut(s) 158, 274, 297, 770, 947, 1043
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 549, 586
HpyF3I CTNAG 2 cut(s) 161, 630
HpySE526I ACGT 2 cut(s) 115, 1018
Hsp92I GRCGYC 1 cut(s) 558
Hsp92II CATG 7 cut(s) 176, 212, 356, 445, 556, 709, 1153
HspAI GCGC 1 cut(s) 430
Ksp22I TGATCA 2 cut(s) 349, 928
LmnI GCTCC 2 cut(s) 741, 911
Lsp1109I GCAGC 6 cut(s) 247, 258, 570, 573, 592, 754
MaeI CTAG 4 cut(s) 110, 390, 1062, 1100
MaeII ACGT 2 cut(s) 115, 1018
MaeIII GTNAC 1 cut(s) 3
MfeI CAATTG 2 cut(s) 372, 399
MhlI GDGCHC 2 cut(s) 686, 949
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 6 cut(s) 180, 364, 372, 399, 883, 893
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 2 cut(s) 554, 998
MmeI TCCRAC 3 cut(s) 167, 514, 621
Mox20I TGGCCA 1 cut(s) 207
MscI TGGCCA 1 cut(s) 207
MseI TTAA 6 cut(s) 36, 474, 563, 777, 966, 1143
MslI CAYNNNNRTG 2 cut(s) 252, 1135
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 2 cut(s) 271, 583
MspI CCGG 2 cut(s) 139, 903
MspR9I CCNGG 2 cut(s) 140, 904
MunI CAATTG 2 cut(s) 372, 399
MvnI CGCG 1 cut(s) 1107
MwoI GCNNNNNNNGC 3 cut(s) 110, 549, 586
NciI CCSGG 2 cut(s) 140, 904
NheI GCTAGC 1 cut(s) 109
NlaIII CATG 7 cut(s) 176, 212, 356, 445, 556, 709, 1153
NlaIV GGNNCC 2 cut(s) 43, 61
NmeAIII GCCGAG 1 cut(s) 743
NmuCI GTSAC 1 cut(s) 3
PagI TCATGA 2 cut(s) 172, 352
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 540
PfeI GAWTC 1 cut(s) 716
PfoI TCCNGGA 1 cut(s) 902
PkrI GCNGC 8 cut(s) 146, 234, 237, 273, 582, 585, 588, 769
PleI GAGTC 2 cut(s) 553, 998
PmaCI CACGTG 2 cut(s) 116, 1019
PmlI CACGTG 2 cut(s) 116, 1019
PpsI GAGTC 2 cut(s) 553, 998
Ppu21I YACGTR 2 cut(s) 116, 1019
PshBI ATTAAT 2 cut(s) 474, 966
PspCI CACGTG 2 cut(s) 116, 1019
PspN4I GGNNCC 2 cut(s) 43, 61
PspPI GGNCC 2 cut(s) 709, 829
PstNI CAGNNNCTG 1 cut(s) 586
PvuII CAGCTG 2 cut(s) 271, 583
RsaI GTAC 3 cut(s) 245, 266, 641
RsaNI GTAC 3 cut(s) 244, 265, 640
RseI CAYNNNNRTG 2 cut(s) 252, 1135
SalI GTCGAC 1 cut(s) 50
SaqAI TTAA 6 cut(s) 36, 474, 563, 777, 966, 1143
SatI GCNGC 8 cut(s) 145, 233, 236, 272, 581, 584, 587, 768
Sau96I GGNCC 2 cut(s) 709, 829
SchI GAGTC 2 cut(s) 554, 998
ScrFI CCNGG 2 cut(s) 140, 904
SduI GDGCHC 2 cut(s) 686, 949
SinI GGWCC 1 cut(s) 829
SmiMI CAYNNNNRTG 2 cut(s) 252, 1135
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
SpeI ACTAGT 1 cut(s) 389
Sse9I AATT 6 cut(s) 180, 364, 372, 399, 883, 893
SsiI CCGC 3 cut(s) 144, 233, 1105
SspMI CTAG 4 cut(s) 110, 390, 1062, 1100
StyD4I CCNGG 2 cut(s) 138, 902
StyI CCWWGG 2 cut(s) 816, 952
TaaI ACNGT 6 cut(s) 46, 56, 92, 227, 243, 251
TaiI ACGT 2 cut(s) 118, 1021
TaqI TCGA 3 cut(s) 51, 87, 97
TasI AATT 6 cut(s) 180, 364, 372, 399, 883, 893
TatI WGTACW 1 cut(s) 639
TauI GCSGC 2 cut(s) 147, 235
TfiI GAWTC 1 cut(s) 716
Tru1I TTAA 6 cut(s) 36, 474, 563, 777, 966, 1143
Tru9I TTAA 6 cut(s) 36, 474, 563, 777, 966, 1143
TscAI CASTG 1 cut(s) 208
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 6 cut(s) 235, 271, 580, 583, 586, 767
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 7 cut(s) 192, 225, 270, 486, 694, 1119, 1134
TspRI CASTG 1 cut(s) 208
VneI GTGCAC 1 cut(s) 945
VpaK11BI GGWCC 1 cut(s) 829
VspI ATTAAT 2 cut(s) 474, 966
XagI CCTNNNNNAGG 1 cut(s) 1065
XapI RAATTY 1 cut(s) 180
XcmI CCANNNNNNNNNTGG 1 cut(s) 823
XmiI GTMKAC 1 cut(s) 51
XspI CTAG 4 cut(s) 110, 390, 1062, 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.