MD06G1014400.v1.1

Protein Brevis radix-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005297
Physical Location & Seq
Reverse (-)
1829232 .. 1835824
6593 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1014400.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGTTTACGTGCATAGCATGTACGAAGCAAACGGACGATGGAGGAGAAGAAGGAGCAGCGCGTGGCAGCGGCACCCCAAATACAAAAGAAGCCGTCAAAAGCCTGACGGCGCAGATTAAGGATATGGCACTGAAATTCTCTGGTGCGTACAAGCAATGCAAACCATGCACAGGGTCAAGCAATTATAAGAAAGGACGGCCGAGATACCCGGATTTTGATACTGCTTCTGAAGGGATGCCGTACCCATATATGGGAGGTGCAAGCACAAGTTCTACACCTGCTTGGGACTTCACCTCTAATCATCAGTTGGGCGCAAGATCGGACTCTCGATTTACTAGACCATATGGTGGCGGTGAGCGTGCATCAATATCAGCACAGTCTTGTGATGTTGTGCTAGAGGATGAGGATGAGCCTAAGGAGTGGATGGCACAGGTGGAACCCGGTGTACACATTACTTTTGTATCTCTTCCTAATGGGGGAAATGATCTCAAACGAATTCGTTTCAGCCGAGAGATGTTCAACAAATGGCAAGCGCAGCGATGGTGGGGTGAGAATTATGACCGCATCATGGAGCTCTACAATGTCCAGAGATTCAACCGGCAAGCTCTTCACACTCCCCCAAGGTCCGAAGATGAGCAGCAGAGAGATTCTTCTTACTCAAGGATGGGATCTGCTATGGAAAGCCCTATGGCTCCATCAGTGAACAAAGATTGGACACCGAGGCACAACTATAAACCGTCTGGAACTGGAAGTAGAGGGTATTTCCCCTCTGATGGTTTGGATCATGGTGGTGGCAGTGGTGGTCACTACTATAATGCTGGTGGGTCAAGTGCTTATGGAGCAGGTGGCCACAAGGGAGAGGCATCATCTTTTGATGCATCGCGGACAACAACGTCCTCTCGAGATGAAGCTTCAGTCTCAATTAGCAATGCTAGTGAAGTGGAGTCGGAATGGGTTGAGGAAGATGAGCCTGGGGTGTATATAACCATTAGGCAGCTAGCGGATGGCACTCGGGAGCTCAGGCGTGTCAGATTCAGCCGCGAAAGGTTTGGGGAGATGAATGCGAAGACATGGTGGGAAGAAAACAGAGAGAGAATACAAGCACAATACCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRX BRX_N

Protein Analysis

372

Amino Acids

41.37

Weight (kDa)

5.91

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRX_N PF13713 23 - 56 1.8e-17 Transcription factor BRX N-terminal domain
BRX PF08381 139 - 193 3.4e-26 Transcription factor regulating root and shoot growth via Pin3
BRX PF08381 315 - 370 4.9e-27 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 186
AarI CACCTGC 2 cut(s) 286, 833
AasI GACNNNNNNGTC 1 cut(s) 892
Acc36I ACCTGC 2 cut(s) 286, 833
AccB1I GGYRCC 1 cut(s) 71
AccB7I CCANNNNNTGG 1 cut(s) 347
AccII CGCG 3 cut(s) 61, 883, 1041
AciI CCGC 6 cut(s) 69, 351, 562, 883, 1001, 1039
AclWI GGATC 2 cut(s) 676, 789
AcoI YGGCCR 2 cut(s) 197, 847
AcsI RAATTY 2 cut(s) 134, 495
AcuI CTGAAG 2 cut(s) 249, 897
AfaI GTAC 4 cut(s) 22, 149, 242, 447
AfiI CCNNNNNNNGG 7 cut(s) 170, 250, 251, 347, 476, 568, 773
AgsI TTSAA 2 cut(s) 520, 595
AjnI CCWGG 1 cut(s) 970
AluBI AGCT 5 cut(s) 574, 605, 911, 997, 1018
AluI AGCT 5 cut(s) 574, 605, 911, 997, 1018
Alw21I GWGCWC 2 cut(s) 576, 1020
Alw26I GTCTC 1 cut(s) 922
AlwI GGATC 2 cut(s) 676, 789
Ama87I CYCGRG 2 cut(s) 900, 1011
AoxI GGCC 2 cut(s) 197, 847
ApeKI GCWGC 5 cut(s) 56, 66, 535, 637, 994
ApoI RAATTY 2 cut(s) 134, 495
AspLEI GCGC 4 cut(s) 61, 112, 314, 535
AspS9I GGNCC 1 cut(s) 624
AsuC2I CCSGG 2 cut(s) 209, 441
AsuHPI GGTGA 3 cut(s) 283, 365, 560
AsuNHI GCTAGC 1 cut(s) 997
AvaI CYCGRG 2 cut(s) 900, 1011
AvaII GGWCC 1 cut(s) 624
AxyI CCTNAGG 1 cut(s) 414
BaeI ACNNNNGTAYC 2 cut(s) 444, 477
BalI TGGCCA 1 cut(s) 849
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 2 cut(s) 576, 1020
BbsI GAAGAC 1 cut(s) 1073
Bbv12I GWGCWC 2 cut(s) 576, 1020
BbvI GCAGC 5 cut(s) 68, 78, 547, 649, 1006
BccI CCATC 7 cut(s) 32, 418, 534, 658, 703, 767, 998
BceAI ACGGC 4 cut(s) 77, 123, 212, 223
BciT130I CCWGG 1 cut(s) 972
BcnI CCSGG 2 cut(s) 209, 441
BcoDI GTCTC 1 cut(s) 922
BfaI CTAG 4 cut(s) 336, 395, 933, 998
BfuAI ACCTGC 2 cut(s) 286, 833
BisI GCNGC 7 cut(s) 57, 67, 70, 536, 638, 995, 1039
BlsI GCNGC 7 cut(s) 58, 68, 71, 537, 639, 996, 1040
Bme1390I CCNGG 3 cut(s) 209, 441, 972
Bme18I GGWCC 1 cut(s) 624
BmeT110I CYCGRG 2 cut(s) 900, 1011
BmgT120I GGNCC 1 cut(s) 624
BmiI GGNNCC 3 cut(s) 73, 438, 693
BmrFI CCNGG 3 cut(s) 209, 441, 972
BmsI GCATC 6 cut(s) 225, 371, 573, 865, 872, 887
BmtI GCTAGC 1 cut(s) 1001
BpiI GAAGAC 1 cut(s) 1073
Bpu10I CCTNAGC 1 cut(s) 1019
BpuEI CTTGAG 1 cut(s) 643
BpuMI CCSGG 2 cut(s) 209, 441
BsaAI YACGTR 1 cut(s) 9
BsaJI CCNNGG 3 cut(s) 620, 719, 971
Bsc4I CCNNNNNNNGG 7 cut(s) 170, 250, 251, 347, 476, 568, 773
Bse118I RCCGGY 1 cut(s) 597
Bse1I ACTGG 1 cut(s) 751
Bse21I CCTNAGG 1 cut(s) 414
Bse3DI GCAATG 2 cut(s) 161, 934
BseBI CCWGG 1 cut(s) 972
BseDI CCNNGG 3 cut(s) 620, 719, 971
BseGI GGATG 6 cut(s) 240, 406, 412, 429, 669, 1009
BseLI CCNNNNNNNGG 7 cut(s) 170, 250, 251, 347, 476, 568, 773
BseMI GCAATG 2 cut(s) 161, 934
BseMII CTCAG 1 cut(s) 1033
BseNI ACTGG 1 cut(s) 751
BseRI GAGGAG 1 cut(s) 57
BseX3I CGGCCG 1 cut(s) 197
BseXI GCAGC 5 cut(s) 68, 78, 547, 649, 1006
Bsh1236I CGCG 3 cut(s) 61, 883, 1041
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 2 cut(s) 199, 849
BshNI GGYRCC 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 2 cut(s) 576, 1020
BsiHKCI CYCGRG 2 cut(s) 900, 1011
BsiSI CCGG 3 cut(s) 209, 441, 598
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 7 cut(s) 170, 250, 251, 347, 476, 568, 773
BsmAI GTCTC 1 cut(s) 922
BsmFI GGGAC 1 cut(s) 299
BsmI GAATGC 1 cut(s) 1066
BsnI GGCC 2 cut(s) 199, 849
BsoBI CYCGRG 2 cut(s) 900, 1011
Bsp1286I GDGCHC 2 cut(s) 576, 1020
Bsp1407I TGTACA 1 cut(s) 445
Bsp143I GATC 4 cut(s) 317, 484, 668, 781
BspACI CCGC 6 cut(s) 69, 351, 562, 883, 1001, 1039
BspANI GGCC 2 cut(s) 199, 849
BspCNI CTCAG 1 cut(s) 1032
BspFNI CGCG 3 cut(s) 61, 883, 1041
BspLI GGNNCC 3 cut(s) 73, 438, 693
BspMI ACCTGC 2 cut(s) 286, 833
BspOI GCTAGC 1 cut(s) 1001
BspPI GGATC 2 cut(s) 676, 789
BspQI GCTCTTC 1 cut(s) 612
BspT107I GGYRCC 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 161, 934
BsrFI RCCGGY 1 cut(s) 597
BsrGI TGTACA 1 cut(s) 445
BsrI ACTGG 1 cut(s) 751
BssAI RCCGGY 1 cut(s) 597
BssECI CCNNGG 3 cut(s) 620, 719, 971
BssMI GATC 4 cut(s) 317, 484, 668, 781
BssT1I CCWWGG 1 cut(s) 620
Bst2UI CCWGG 1 cut(s) 972
Bst4CI ACNGT 2 cut(s) 378, 738
Bst6I CTCTTC 2 cut(s) 471, 612
BstAPI GCANNNNNTGC 1 cut(s) 165
BstAUI TGTACA 1 cut(s) 445
BstBAI YACGTR 1 cut(s) 9
BstC8I GCNNGC 5 cut(s) 262, 360, 531, 603, 999
BstDEI CTNAG 2 cut(s) 414, 1019
BstF5I GGATG 6 cut(s) 240, 406, 412, 429, 669, 1009
BstFNI CGCG 3 cut(s) 61, 883, 1041
BstHHI GCGC 4 cut(s) 61, 112, 314, 535
BstKTI GATC 4 cut(s) 320, 487, 671, 784
BstMAI GTCTC 1 cut(s) 922
BstMBI GATC 4 cut(s) 317, 484, 668, 781
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 3 cut(s) 165, 535, 839
BstNI CCWGG 1 cut(s) 972
BstNSI RCATGY 1 cut(s) 21
BstSCI CCNGG 3 cut(s) 207, 439, 970
BstUI CGCG 3 cut(s) 61, 883, 1041
BstV1I GCAGC 5 cut(s) 68, 78, 547, 649, 1006
BstV2I GAAGAC 1 cut(s) 1073
BstX2I RGATCY 1 cut(s) 668
BstYI RGATCY 1 cut(s) 668
BstZI CGGCCG 1 cut(s) 197
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 2 cut(s) 199, 849
BtgZI GCGATG 2 cut(s) 553, 864
BtsCI GGATG 6 cut(s) 240, 406, 412, 429, 669, 1009
BtsI GCAGTG 1 cut(s) 802
BtsIMutI CAGTG 3 cut(s) 128, 705, 802
BveI ACCTGC 2 cut(s) 286, 833
Cac8I GCNNGC 5 cut(s) 262, 360, 531, 603, 999
CfoI GCGC 4 cut(s) 61, 112, 314, 535
Cfr10I RCCGGY 1 cut(s) 597
Cfr13I GGNCC 1 cut(s) 624
Csp6I GTAC 4 cut(s) 21, 148, 241, 446
CviAII CATG 5 cut(s) 18, 165, 568, 785, 1071
CviQI GTAC 4 cut(s) 21, 148, 241, 446
DdeI CTNAG 2 cut(s) 414, 1019
DpnI GATC 4 cut(s) 319, 486, 670, 783
DpnII GATC 4 cut(s) 317, 484, 668, 781
DrdI GACNNNNNNGTC 1 cut(s) 892
DseDI GACNNNNNNGTC 1 cut(s) 892
EaeI YGGCCR 2 cut(s) 197, 847
EagI CGGCCG 1 cut(s) 197
Eam1104I CTCTTC 2 cut(s) 471, 612
EarI CTCTTC 2 cut(s) 471, 612
Ecl136II GAGCTC 2 cut(s) 574, 1018
EclXI CGGCCG 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 620
Eco24I GRGCYC 2 cut(s) 576, 1020
Eco47I GGWCC 1 cut(s) 624
Eco52I CGGCCG 1 cut(s) 197
Eco53kI GAGCTC 2 cut(s) 574, 1018
Eco57I CTGAAG 2 cut(s) 249, 897
Eco81I CCTNAGG 1 cut(s) 414
Eco88I CYCGRG 2 cut(s) 900, 1011
EcoICRI GAGCTC 2 cut(s) 574, 1018
EcoRI GAATTC 1 cut(s) 495
EcoRII CCWGG 1 cut(s) 970
EcoT14I CCWWGG 1 cut(s) 620
EcoT22I ATGCAT 1 cut(s) 880
EcoT38I GRGCYC 2 cut(s) 576, 1020
ErhI CCWWGG 1 cut(s) 620
FaeI CATG 5 cut(s) 21, 168, 571, 788, 1074
FaqI GGGAC 1 cut(s) 299
FatI CATG 5 cut(s) 17, 164, 567, 784, 1070
FauNDI CATATG 1 cut(s) 343
Fnu4HI GCNGC 7 cut(s) 57, 67, 70, 536, 638, 995, 1039
FokI GGATG 6 cut(s) 247, 413, 419, 436, 676, 1016
FriOI GRGCYC 2 cut(s) 576, 1020
Fsp4HI GCNGC 7 cut(s) 57, 67, 70, 536, 638, 995, 1039
FspBI CTAG 4 cut(s) 336, 395, 933, 998
GlaI GCGC 4 cut(s) 60, 111, 313, 534
GluI GCNGC 7 cut(s) 57, 67, 70, 536, 638, 995, 1039
HaeIII GGCC 2 cut(s) 199, 849
HapII CCGG 3 cut(s) 209, 441, 598
HhaI GCGC 4 cut(s) 61, 112, 314, 535
Hin1II CATG 5 cut(s) 21, 168, 571, 788, 1074
Hin6I GCGC 4 cut(s) 59, 110, 312, 533
HinP1I GCGC 4 cut(s) 59, 110, 312, 533
HindIII AAGCTT 1 cut(s) 909
HinfI GANTC 5 cut(s) 323, 591, 647, 944, 1032
HpaII CCGG 3 cut(s) 209, 441, 598
HphI GGTGA 3 cut(s) 283, 365, 560
Hpy166II GTNNAC 4 cut(s) 6, 446, 448, 703
Hpy188I TCNGA 6 cut(s) 229, 322, 628, 772, 949, 1031
Hpy188III TCNNGA 6 cut(s) 327, 586, 741, 900, 902, 1013
Hpy8I GTNNAC 4 cut(s) 6, 446, 448, 703
HpyAV CCTTC 2 cut(s) 44, 224
HpyCH4III ACNGT 2 cut(s) 378, 738
HpyCH4IV ACGT 2 cut(s) 8, 893
HpyCH4V TGCA 6 cut(s) 12, 159, 168, 260, 362, 878
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 535, 839
HpyF3I CTNAG 2 cut(s) 414, 1019
HpySE526I ACGT 2 cut(s) 8, 893
Hsp92II CATG 5 cut(s) 21, 168, 571, 788, 1074
HspAI GCGC 4 cut(s) 59, 110, 312, 533
Kzo9I GATC 4 cut(s) 317, 484, 668, 781
LguI GCTCTTC 1 cut(s) 612
LmnI GCTCC 5 cut(s) 53, 571, 697, 839, 1015
Lsp1109I GCAGC 5 cut(s) 68, 78, 547, 649, 1006
LweI GCATC 6 cut(s) 225, 371, 573, 865, 872, 887
MaeI CTAG 4 cut(s) 336, 395, 933, 998
MaeII ACGT 2 cut(s) 8, 893
MaeIII GTNAC 1 cut(s) 803
MalI GATC 4 cut(s) 319, 486, 670, 783
MboI GATC 4 cut(s) 317, 484, 668, 781
MboII GAAGA 8 cut(s) 59, 458, 599, 641, 642, 974, 1078, 1091
MflI RGATCY 1 cut(s) 668
MhlI GDGCHC 2 cut(s) 576, 1020
MlsI TGGCCA 1 cut(s) 849
MluCI AATT 5 cut(s) 134, 181, 495, 553, 921
MluNI TGGCCA 1 cut(s) 849
MlyI GAGTC 2 cut(s) 317, 953
MmeI TCCRAC 1 cut(s) 927
Mox20I TGGCCA 1 cut(s) 849
Mph1103I ATGCAT 1 cut(s) 880
MscI TGGCCA 1 cut(s) 849
MseI TTAA 2 cut(s) 117, 1114
MslI CAYNNNNRTG 1 cut(s) 789
Msp20I TGGCCA 1 cut(s) 849
MspA1I CMGCKG 1 cut(s) 69
MspI CCGG 3 cut(s) 209, 441, 598
MspR9I CCNGG 3 cut(s) 209, 441, 972
Mva1269I GAATGC 1 cut(s) 1066
MvaI CCWGG 1 cut(s) 972
MvnI CGCG 3 cut(s) 61, 883, 1041
MwoI GCNNNNNNNGC 3 cut(s) 165, 535, 839
NciI CCSGG 2 cut(s) 209, 441
NdeI CATATG 1 cut(s) 343
NdeII GATC 4 cut(s) 317, 484, 668, 781
NheI GCTAGC 1 cut(s) 997
NlaIII CATG 5 cut(s) 21, 168, 571, 788, 1074
NlaIV GGNNCC 3 cut(s) 73, 438, 693
NmeAIII GCCGAG 2 cut(s) 225, 533
NmuCI GTSAC 1 cut(s) 803
NsiI ATGCAT 1 cut(s) 880
NspI RCATGY 1 cut(s) 21
PaeR7I CTCGAG 1 cut(s) 900
PaqCI CACCTGC 2 cut(s) 286, 833
PciSI GCTCTTC 1 cut(s) 612
PcsI WCGNNNNNNNCGW 1 cut(s) 505
PctI GAATGC 1 cut(s) 1066
PfeI GAWTC 3 cut(s) 591, 647, 1032
PflMI CCANNNNNTGG 1 cut(s) 347
PkrI GCNGC 7 cut(s) 58, 68, 71, 537, 639, 996, 1040
PleI GAGTC 2 cut(s) 317, 952
PpsI GAGTC 2 cut(s) 317, 952
Ppu21I YACGTR 1 cut(s) 9
PsiI TTATAA 1 cut(s) 186
Psp124BI GAGCTC 2 cut(s) 576, 1020
Psp6I CCWGG 1 cut(s) 970
PspGI CCWGG 1 cut(s) 970
PspN4I GGNNCC 3 cut(s) 73, 438, 693
PspPI GGNCC 1 cut(s) 624
PsrI GAACNNNNNNTAC 2 cut(s) 429, 461
PsuI RGATCY 1 cut(s) 668
RsaI GTAC 4 cut(s) 22, 149, 242, 447
RsaNI GTAC 4 cut(s) 21, 148, 241, 446
RseI CAYNNNNRTG 1 cut(s) 789
SacI GAGCTC 2 cut(s) 576, 1020
SapI GCTCTTC 1 cut(s) 612
SaqAI TTAA 2 cut(s) 117, 1114
SatI GCNGC 7 cut(s) 57, 67, 70, 536, 638, 995, 1039
Sau3AI GATC 4 cut(s) 317, 484, 668, 781
Sau96I GGNCC 1 cut(s) 624
SchI GAGTC 2 cut(s) 317, 953
ScrFI CCNGG 3 cut(s) 209, 441, 972
SduI GDGCHC 2 cut(s) 576, 1020
SfaNI GCATC 6 cut(s) 225, 371, 573, 865, 872, 887
Sfr274I CTCGAG 1 cut(s) 900
SinI GGWCC 1 cut(s) 624
SlaI CTCGAG 1 cut(s) 900
SmiMI CAYNNNNRTG 1 cut(s) 789
SmlI CTYRAG 2 cut(s) 658, 900
SmoI CTYRAG 2 cut(s) 658, 900
Sse9I AATT 5 cut(s) 134, 181, 495, 553, 921
SsiI CCGC 6 cut(s) 69, 351, 562, 883, 1001, 1039
SspMI CTAG 4 cut(s) 336, 395, 933, 998
SstI GAGCTC 2 cut(s) 576, 1020
StyD4I CCNGG 3 cut(s) 207, 439, 970
StyI CCWWGG 1 cut(s) 620
TaaI ACNGT 2 cut(s) 378, 738
TaiI ACGT 2 cut(s) 11, 896
TaqI TCGA 2 cut(s) 328, 901
TasI AATT 5 cut(s) 134, 181, 495, 553, 921
TatI WGTACW 1 cut(s) 445
TauI GCSGC 2 cut(s) 72, 1041
TfiI GAWTC 3 cut(s) 591, 647, 1032
Tru1I TTAA 2 cut(s) 117, 1114
Tru9I TTAA 2 cut(s) 117, 1114
TscAI CASTG 3 cut(s) 135, 705, 802
TseFI GTSAC 1 cut(s) 803
TseI GCWGC 5 cut(s) 56, 66, 535, 637, 994
Tsp45I GTSAC 1 cut(s) 803
TspDTI ATGAA 2 cut(s) 921, 1073
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 3 cut(s) 135, 705, 802
Van91I CCANNNNNTGG 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 624
XapI RAATTY 2 cut(s) 134, 495
XceI RCATGY 1 cut(s) 21
XhoI CTCGAG 1 cut(s) 900
XspI CTAG 4 cut(s) 336, 395, 933, 998
Zsp2I ATGCAT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.