MD06G1036800.v1.1

Endoplasmic reticulum-based factor for assembly of V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003225
Physical Location & Seq
Forward (+)
4690441 .. 4696360
5920 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1036800.v1.1.491

Sequence Viewer

Length: 885 bp
ATGTGTGCTTCATCTTCTCCTTCGCCTTCATCATCTCCTTCCCTTTACTCATTGCCATCATCGCCGTCTCCAACTCTCTTTGATCTTATGCTCGATCGCCGAAACGCAACTAGAGACAGCGGGCTGAAGACGCGTCTAACCGGCACACAAAGGTTCTATTTACCATCTGGATATTGTCTATCTTCTCATTTTCCAGTTTCCAAGGTTTGGAGTATTGAGCTCGATTGCATAACACCTTGCCGAGTTGTGAAAATGAGCAGTGATCCGATGAACCCACCTGGACTGGTCGTATCGACCACCGAACCCATCCTCTCATTCCTCACCTCAGCTTCCACAGACCCACACCTCTCTCATGAACTCCAGCAAATTGCTTCCAATCTATCCTCCAAACCCACTGTACCGTACAAGTCTCTCCGAGCTCTTTGGGTTGCATCTGATGCCTCGACCCGACCGCACTTGGCCCAACTCTTCTCCGGATGCGAGTTCGTCCTCACCAGCCCCAAACCCAGAGAAAAGAGTGAGGAGTTGAAGGCTAGACTTAAGAAGCTGGCTGAAGTTTTGGAGAGGAATGCGTATGCAGAACTTGTGAAGGATATTACACCTAAAAAAGAAACCACTGAGCCTTTCTCTTCTTACAAGGATCAACTTGGCTTTGGTTTACATGTTGTGGTGACCATGTTTACCTGCTATTTGGTAGGATATGCTGCATTCAGAGCTTTGTTTAACCACAGTCCCGTCATGAATGCTGCTGGAGGCATTCTTGGATTGGTCATTGGCATGCTCGTGGAGACATTTATTTTCATTATTAGAACTTCTAGTCAAGATCTCAGATCATCTTCTTCCACATCCAAGCTAAAGAAGAATCAGTTTCGATGCGGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vma12

Protein Analysis

295

Amino Acids

32.24

Weight (kDa)

9.23

Isoelectric Point (pI)

56.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vma12 PF11712 155 - 262 8.2e-06 Endoplasmic reticulum-based factor for assembly of V-ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 692
AccB7I CCANNNNNTGG 1 cut(s) 207
AccII CGCG 1 cut(s) 133
AccIII TCCGGA 1 cut(s) 473
AciI CCGC 3 cut(s) 120, 452, 876
AclWI GGATC 2 cut(s) 257, 648
AcuI CTGAAG 2 cut(s) 146, 573
AfaI GTAC 2 cut(s) 399, 404
AfiI CCNNNNNNNGG 1 cut(s) 207
AflII CTTAAG 1 cut(s) 539
AflIII ACRYGT 2 cut(s) 131, 661
AgsI TTSAA 1 cut(s) 529
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 6 cut(s) 220, 329, 419, 547, 716, 853
AluI AGCT 6 cut(s) 220, 329, 419, 547, 716, 853
Alw21I GWGCWC 2 cut(s) 222, 421
Alw26I GTCTC 4 cut(s) 72, 108, 414, 782
AlwI GGATC 2 cut(s) 257, 648
Aor13HI TCCGGA 1 cut(s) 473
AoxI GGCC 1 cut(s) 459
ApeKI GCWGC 2 cut(s) 704, 746
AspS9I GGNCC 1 cut(s) 460
AsuHPI GGTGA 3 cut(s) 313, 484, 682
BanII GRGCYC 2 cut(s) 222, 421
BauI CACGAG 1 cut(s) 782
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 2 cut(s) 222, 421
BbvCI CCTCAGC 1 cut(s) 325
BbvI GCAGC 2 cut(s) 691, 733
BccI CCATC 3 cut(s) 64, 172, 314
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 1 cut(s) 279
BcoDI GTCTC 4 cut(s) 72, 108, 414, 782
BfaI CTAG 3 cut(s) 111, 534, 816
BfrI CTTAAG 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 692
BglII AGATCT 1 cut(s) 823
BisI GCNGC 2 cut(s) 705, 747
BlsI GCNGC 2 cut(s) 706, 748
Bme1390I CCNGG 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 460
BmrFI CCNGG 1 cut(s) 279
BmsI GCATC 4 cut(s) 427, 440, 467, 863
BpiI GAAGAC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 611, 643
BpmI CTGGAG 2 cut(s) 344, 771
Bpu10I CCTNAGC 1 cut(s) 325
BsaJI CCNNGG 1 cut(s) 201
BsaWI WCCGGW 1 cut(s) 473
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 2 cut(s) 194, 288
Bse3DI GCAATG 1 cut(s) 50
BseAI TCCGGA 1 cut(s) 473
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 3 cut(s) 306, 482, 845
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMI GCAATG 1 cut(s) 50
BseMII CTCAG 3 cut(s) 339, 609, 841
BseNI ACTGG 2 cut(s) 194, 288
BseRI GAGGAG 1 cut(s) 536
BseXI GCAGC 2 cut(s) 691, 733
Bsh1236I CGCG 1 cut(s) 133
Bsh1285I CGRYCG 2 cut(s) 97, 452
BshFI GGCC 1 cut(s) 461
BsiEI CGRYCG 2 cut(s) 97, 452
BsiHKAI GWGCWC 2 cut(s) 222, 421
BsiSI CCGG 2 cut(s) 141, 474
BslFI GGGAC 1 cut(s) 717
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 4 cut(s) 72, 108, 414, 782
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 1 cut(s) 717
BsmI GAATGC 4 cut(s) 574, 707, 748, 756
BsnI GGCC 1 cut(s) 461
Bsp1286I GDGCHC 2 cut(s) 222, 421
Bsp13I TCCGGA 1 cut(s) 473
Bsp143I GATC 6 cut(s) 82, 94, 262, 640, 823, 830
BspACI CCGC 3 cut(s) 120, 452, 876
BspANI GGCC 1 cut(s) 461
BspCNI CTCAG 3 cut(s) 338, 610, 840
BspEI TCCGGA 1 cut(s) 473
BspFNI CGCG 1 cut(s) 133
BspHI TCATGA 2 cut(s) 352, 738
BspMI ACCTGC 1 cut(s) 692
BspPI GGATC 2 cut(s) 257, 648
BspTI CTTAAG 1 cut(s) 539
BsrDI GCAATG 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 2 cut(s) 194, 288
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 6 cut(s) 82, 94, 262, 640, 823, 830
BssSI CACGAG 1 cut(s) 782
BssT1I CCWWGG 1 cut(s) 201
Bst2BI CACGAG 1 cut(s) 782
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 3 cut(s) 397, 402, 731
Bst6I CTCTTC 2 cut(s) 473, 634
BstAFI CTTAAG 1 cut(s) 539
BstAPI GCANNNNNTGC 1 cut(s) 437
BstC8I GCNNGC 3 cut(s) 122, 549, 779
BstDEI CTNAG 3 cut(s) 325, 618, 827
BstEII GGTNACC 1 cut(s) 670
BstF5I GGATG 3 cut(s) 306, 482, 845
BstFNI CGCG 1 cut(s) 133
BstKTI GATC 6 cut(s) 85, 97, 265, 643, 826, 833
BstMAI GTCTC 4 cut(s) 72, 108, 414, 782
BstMBI GATC 6 cut(s) 82, 94, 262, 640, 823, 830
BstMCI CGRYCG 2 cut(s) 97, 452
BstMWI GCNNNNNNNGC 4 cut(s) 61, 130, 437, 713
BstNI CCWGG 1 cut(s) 279
BstNSI RCATGY 2 cut(s) 665, 781
BstPI GGTNACC 1 cut(s) 670
BstSCI CCNGG 1 cut(s) 277
BstUI CGCG 1 cut(s) 133
BstV1I GCAGC 2 cut(s) 691, 733
BstV2I GAAGAC 1 cut(s) 134
BstX2I RGATCY 1 cut(s) 823
BstYI RGATCY 1 cut(s) 823
BsuRI GGCC 1 cut(s) 461
BtgZI GCGATG 1 cut(s) 45
BtsCI GGATG 3 cut(s) 306, 482, 845
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 3 cut(s) 265, 393, 615
BveI ACCTGC 1 cut(s) 692
Cac8I GCNNGC 3 cut(s) 122, 549, 779
CciI TCATGA 2 cut(s) 352, 738
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 460
CseI GACGC 2 cut(s) 122, 139
Csp6I GTAC 2 cut(s) 398, 403
CviAII CATG 5 cut(s) 353, 662, 676, 739, 778
CviQI GTAC 2 cut(s) 398, 403
DdeI CTNAG 3 cut(s) 325, 618, 827
DpnI GATC 6 cut(s) 84, 96, 264, 642, 825, 832
DpnII GATC 6 cut(s) 82, 94, 262, 640, 823, 830
Eam1104I CTCTTC 2 cut(s) 473, 634
EarI CTCTTC 2 cut(s) 473, 634
Ecl136II GAGCTC 2 cut(s) 220, 419
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 2 cut(s) 222, 421
Eco53kI GAGCTC 2 cut(s) 220, 419
Eco57I CTGAAG 2 cut(s) 146, 573
Eco91I GGTNACC 1 cut(s) 670
EcoICRI GAGCTC 2 cut(s) 220, 419
EcoO65I GGTNACC 1 cut(s) 670
EcoRII CCWGG 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 2 cut(s) 222, 421
ErhI CCWWGG 1 cut(s) 201
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 5 cut(s) 356, 665, 679, 742, 781
FaiI YATR 9 cut(s) 89, 230, 354, 576, 663, 677, 702, 740, 779
FaqI GGGAC 1 cut(s) 717
FatI CATG 5 cut(s) 352, 661, 675, 738, 777
FauI CCCGC 1 cut(s) 113
Fnu4HI GCNGC 2 cut(s) 705, 747
FokI GGATG 3 cut(s) 293, 489, 832
FriOI GRGCYC 2 cut(s) 222, 421
Fsp4HI GCNGC 2 cut(s) 705, 747
FspBI CTAG 3 cut(s) 111, 534, 816
GluI GCNGC 2 cut(s) 705, 747
GsuI CTGGAG 2 cut(s) 344, 771
HaeIII GGCC 1 cut(s) 461
HapII CCGG 2 cut(s) 141, 474
HgaI GACGC 2 cut(s) 122, 139
Hin1II CATG 5 cut(s) 356, 665, 679, 742, 781
HinfI GANTC 1 cut(s) 862
HpaII CCGG 2 cut(s) 141, 474
HphI GGTGA 3 cut(s) 313, 484, 682
Hpy166II GTNNAC 2 cut(s) 659, 681
Hpy188I TCNGA 5 cut(s) 267, 416, 436, 713, 830
Hpy188III TCNNGA 5 cut(s) 168, 353, 474, 739, 821
Hpy8I GTNNAC 2 cut(s) 659, 681
HpyAV CCTTC 5 cut(s) 30, 36, 48, 523, 583
HpyCH4III ACNGT 3 cut(s) 397, 402, 731
HpyCH4V TGCA 4 cut(s) 228, 431, 578, 707
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 130, 437, 713
HpyF3I CTNAG 3 cut(s) 325, 618, 827
Hsp92II CATG 5 cut(s) 356, 665, 679, 742, 781
Kpn2I TCCGGA 1 cut(s) 473
Kzo9I GATC 6 cut(s) 82, 94, 262, 640, 823, 830
Lsp1109I GCAGC 2 cut(s) 691, 733
LweI GCATC 4 cut(s) 427, 440, 467, 863
MaeI CTAG 3 cut(s) 111, 534, 816
MaeIII GTNAC 1 cut(s) 670
MalI GATC 6 cut(s) 84, 96, 264, 642, 825, 832
MboI GATC 6 cut(s) 82, 94, 262, 640, 823, 830
MboII GAAGA 8 cut(s) 6, 139, 174, 460, 621, 828, 831, 871
MflI RGATCY 1 cut(s) 823
MhlI GDGCHC 2 cut(s) 222, 421
MluCI AATT 1 cut(s) 366
MluI ACGCGT 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 95
MroI TCCGGA 1 cut(s) 473
MseI TTAA 2 cut(s) 540, 723
MslI CAYNNNNRTG 2 cut(s) 776, 782
MspA1I CMGCKG 1 cut(s) 120
MspCI CTTAAG 1 cut(s) 539
MspI CCGG 2 cut(s) 141, 474
MspR9I CCNGG 1 cut(s) 279
Mva1269I GAATGC 4 cut(s) 574, 707, 748, 756
MvaI CCWGG 1 cut(s) 279
MvnI CGCG 1 cut(s) 133
MwoI GCNNNNNNNGC 4 cut(s) 61, 130, 437, 713
NdeII GATC 6 cut(s) 82, 94, 262, 640, 823, 830
NlaIII CATG 5 cut(s) 356, 665, 679, 742, 781
NmeAIII GCCGAG 1 cut(s) 266
NmuCI GTSAC 1 cut(s) 670
NspI RCATGY 2 cut(s) 665, 781
PaeI GCATGC 1 cut(s) 781
PagI TCATGA 2 cut(s) 352, 738
PciI ACATGT 1 cut(s) 661
PctI GAATGC 4 cut(s) 574, 707, 748, 756
PfeI GAWTC 1 cut(s) 862
PflMI CCANNNNNTGG 1 cut(s) 207
PkrI GCNGC 2 cut(s) 706, 748
Ple19I CGATCG 1 cut(s) 97
PscI ACATGT 1 cut(s) 661
Psp124BI GAGCTC 2 cut(s) 222, 421
Psp6I CCWGG 1 cut(s) 277
PspEI GGTNACC 1 cut(s) 670
PspGI CCWGG 1 cut(s) 277
PspPI GGNCC 1 cut(s) 460
PsuI RGATCY 1 cut(s) 823
PvuI CGATCG 1 cut(s) 97
RsaI GTAC 2 cut(s) 399, 404
RsaNI GTAC 2 cut(s) 398, 403
RseI CAYNNNNRTG 2 cut(s) 776, 782
SacI GAGCTC 2 cut(s) 222, 421
SaqAI TTAA 2 cut(s) 540, 723
SatI GCNGC 2 cut(s) 705, 747
Sau3AI GATC 6 cut(s) 82, 94, 262, 640, 823, 830
Sau96I GGNCC 1 cut(s) 460
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 2 cut(s) 222, 421
SfaNI GCATC 4 cut(s) 427, 440, 467, 863
SmiMI CAYNNNNRTG 2 cut(s) 776, 782
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
SphI GCATGC 1 cut(s) 781
Sse9I AATT 1 cut(s) 366
SsiI CCGC 3 cut(s) 120, 452, 876
SspMI CTAG 3 cut(s) 111, 534, 816
SstI GAGCTC 2 cut(s) 222, 421
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 3 cut(s) 397, 402, 731
TaqI TCGA 5 cut(s) 93, 222, 293, 443, 871
TasI AATT 1 cut(s) 366
TfiI GAWTC 1 cut(s) 862
Tru1I TTAA 2 cut(s) 540, 723
Tru9I TTAA 2 cut(s) 540, 723
TscAI CASTG 3 cut(s) 265, 400, 622
TseFI GTSAC 1 cut(s) 670
TseI GCWGC 2 cut(s) 704, 746
Tsp45I GTSAC 1 cut(s) 670
TspDTI ATGAA 5 cut(s) 18, 284, 369, 755, 790
TspRI CASTG 3 cut(s) 265, 400, 622
Van91I CCANNNNNTGG 1 cut(s) 207
Vha464I CTTAAG 1 cut(s) 539
XceI RCATGY 2 cut(s) 665, 781
XspI CTAG 3 cut(s) 111, 534, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.