Rh1AG349500

Endoplasmic reticulum-based factor for assembly of V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0003225
Physical Location & Seq
Forward (+)
58640074 .. 58670519
30446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG349500.1

Sequence Viewer

Length: 984 bp
ATGAAGGCCGCAACGGAGATTTTGGGAGATGGAGGATTGGGGTCAGCTTACAAGGATGTGATGGGTACTGGGTTGTCTGTGGTGGTGAAGAGGATGAGGGAGATGAACAGAAGAGTTTGTTGGGTAGAAGAGAAACTTGAGTCTGGACAGGGGAAGACAACTTTCCGCCGTTTCTTTTCTAGATTTTGTACACCAATCTTTTTGGAGTCATTCGTTCTAACATTTTTAGCTGAGTGGGGAGATCGTAGCCAAATAGCGACAATCGCTGTCACCGTTAAACTCACTCCAACCTTTGATCGATTTCTCTCTCGAACCATTTTTCCCGGAGGCCCCCAAAAAGGCGCGAAAATGAGCAGAGACTCTCCGGCACCAGCAACTGGACCCGGACCCGGACTGGTGATATCGACGACGCCACCCATCCTCTCCTTCCTCGTCTCCGCCTCCACCGACCCACACCTCTCCAAGGACCTCCAGCAAACCGCCTCCAAGCTCTCCTCCAAATCCAATGTCCCCTACCGGGCCATCCGCGCCCTCTGGGTCGCTTCCCGGGCCTCCACCCGGCCCCAATTGGTCAAGCTCTTCTCCGGTTCCGGGTTAGTCTTCACCAGCCCCAAGCCCAGAGAAAAGAGTGAGGAGCTGAAGGCGAGGCTGAAGGAGCTGGTGGAGATGTCAGAGAGGAATGCATATGCAGAGCTTGTGAAGGATATAACGCCCCGGAAAGATAGCGCCCCGGAGCCGTTCTCGTCTTACAAGGATCAATTGGGCTTTGGTTTACATGTTGTGGTGACCATGTTTACCGGCTATTTGGTTGGATATGCTGCATTCAGAGCATTGTTTAGCCACAGTCCCGTGATGAATGCTGCTGGAGGCATTCTTGGATTGGTCATTGGCATGCTCGTCGAAACGCTTATTTTCATAATTCAAGCTTCACGCTACGATCTTAAATCATCCTCTTCCACATCCAAGCTAAAGAAGGATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Vma12

Protein Analysis

327

Amino Acids

35.8

Weight (kDa)

9.89

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDT1 PF01169 69 - 93 2.2e-06 Divalent cation/proton antiporter GDT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 367
AccB7I CCANNNNNTGG 1 cut(s) 377
AccII CGCG 2 cut(s) 344, 528
AciI CCGC 5 cut(s) 9, 166, 438, 480, 526
AclWI GGATC 1 cut(s) 762
AcuI CTGAAG 2 cut(s) 659, 671
AcyI GRCGYC 1 cut(s) 410
AfaI GTAC 2 cut(s) 67, 190
AfiI CCNNNNNNNGG 7 cut(s) 338, 377, 389, 463, 517, 558, 591
AflIII ACRYGT 1 cut(s) 775
AgsI TTSAA 1 cut(s) 923
AjuI GAANNNNNNNTTGG 2 cut(s) 103, 135
AloI GAACNNNNNNTCC 2 cut(s) 304, 336
AluBI AGCT 9 cut(s) 47, 230, 490, 577, 637, 658, 694, 926, 967
AluI AGCT 9 cut(s) 47, 230, 490, 577, 637, 658, 694, 926, 967
Alw26I GTCTC 2 cut(s) 351, 439
AlwI GGATC 1 cut(s) 762
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 546
AoxI GGCC 5 cut(s) 6, 328, 519, 549, 560
ApeKI GCWGC 2 cut(s) 818, 860
ArsI GACNNNNNNTTYG 2 cut(s) 492, 524
AspLEI GCGC 3 cut(s) 344, 530, 728
AspS9I GGNCC 7 cut(s) 329, 380, 386, 466, 519, 549, 561
AsuHPI GGTGA 5 cut(s) 97, 262, 409, 595, 796
AvaI CYCGRG 1 cut(s) 546
AvaII GGWCC 3 cut(s) 380, 386, 466
BanI GGYRCC 1 cut(s) 367
BbsI GAAGAC 2 cut(s) 161, 592
BbvI GCAGC 2 cut(s) 805, 847
BccI CCATC 4 cut(s) 23, 55, 425, 530
BceAI ACGGC 2 cut(s) 153, 721
BcgI CGANNNNNNTGC 2 cut(s) 880, 914
BcoDI GTCTC 2 cut(s) 351, 439
BfaI CTAG 1 cut(s) 180
BfoI RGCGCY 1 cut(s) 729
BisI GCNGC 3 cut(s) 9, 819, 861
BlsI GCNGC 3 cut(s) 10, 820, 862
Bme18I GGWCC 3 cut(s) 380, 386, 466
BmeT110I CYCGRG 1 cut(s) 546
BmgT120I GGNCC 7 cut(s) 329, 380, 386, 466, 519, 549, 561
BmiI GGNNCC 7 cut(s) 331, 369, 382, 388, 563, 589, 735
BmrI ACTGGG 1 cut(s) 78
BmuI ACTGGG 1 cut(s) 78
BpiI GAAGAC 2 cut(s) 161, 592
BplI GAGNNNNNCTC 2 cut(s) 725, 757
BpmI CTGGAG 2 cut(s) 455, 885
BpuEI CTTGAG 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 410
BsaJI CCNNGG 4 cut(s) 462, 546, 713, 729
BsaWI WCCGGW 1 cut(s) 584
Bsc4I CCNNNNNNNGG 7 cut(s) 338, 377, 389, 463, 517, 558, 591
Bse118I RCCGGY 1 cut(s) 797
Bse1I ACTGG 3 cut(s) 73, 382, 399
BseCI ATCGAT 1 cut(s) 298
BseDI CCNNGG 4 cut(s) 462, 546, 713, 729
BseGI GGATG 6 cut(s) 61, 99, 417, 522, 947, 959
BseLI CCNNNNNNNGG 7 cut(s) 338, 377, 389, 463, 517, 558, 591
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 3 cut(s) 73, 382, 399
BseRI GAGGAG 2 cut(s) 484, 647
BseXI GCAGC 2 cut(s) 805, 847
Bsh1236I CGCG 2 cut(s) 344, 528
BshFI GGCC 5 cut(s) 8, 330, 521, 551, 562
BshNI GGYRCC 1 cut(s) 367
BshVI ATCGAT 1 cut(s) 298
BsiHKCI CYCGRG 1 cut(s) 546
BslFI GGGAC 2 cut(s) 494, 831
BslI CCNNNNNNNGG 7 cut(s) 338, 377, 389, 463, 517, 558, 591
BsmAI GTCTC 2 cut(s) 351, 439
BsmBI CGTCTC 1 cut(s) 439
BsmFI GGGAC 2 cut(s) 494, 831
BsmI GAATGC 4 cut(s) 685, 821, 862, 870
BsnI GGCC 5 cut(s) 8, 330, 521, 551, 562
BsoBI CYCGRG 1 cut(s) 546
Bsp1407I TGTACA 1 cut(s) 188
Bsp143I GATC 5 cut(s) 241, 295, 754, 937, 976
BspACI CCGC 5 cut(s) 9, 166, 438, 480, 526
BspANI GGCC 5 cut(s) 8, 330, 521, 551, 562
BspCNI CTCAG 1 cut(s) 223
BspDI ATCGAT 1 cut(s) 298
BspFNI CGCG 2 cut(s) 344, 528
BspLI GGNNCC 7 cut(s) 331, 369, 382, 388, 563, 589, 735
BspPI GGATC 1 cut(s) 762
BspQI GCTCTTC 1 cut(s) 584
BspT107I GGYRCC 1 cut(s) 367
BsrFI RCCGGY 1 cut(s) 797
BsrGI TGTACA 1 cut(s) 188
BsrI ACTGG 3 cut(s) 73, 382, 399
BssAI RCCGGY 1 cut(s) 797
BssECI CCNNGG 4 cut(s) 462, 546, 713, 729
BssMI GATC 5 cut(s) 241, 295, 754, 937, 976
BssNI GRCGYC 1 cut(s) 410
BssT1I CCWWGG 1 cut(s) 462
Bst4CI ACNGT 2 cut(s) 274, 845
Bst6I CTCTTC 5 cut(s) 83, 106, 123, 584, 958
BstACI GRCGYC 1 cut(s) 410
BstAUI TGTACA 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 893
BstDEI CTNAG 1 cut(s) 231
BstEII GGTNACC 1 cut(s) 784
BstENI CCTNNNNNAGG 1 cut(s) 461
BstF5I GGATG 6 cut(s) 61, 99, 417, 522, 947, 959
BstFNI CGCG 2 cut(s) 344, 528
BstH2I RGCGCY 1 cut(s) 729
BstHHI GCGC 3 cut(s) 344, 530, 728
BstKTI GATC 5 cut(s) 244, 298, 757, 940, 979
BstMAI GTCTC 2 cut(s) 351, 439
BstMBI GATC 5 cut(s) 241, 295, 754, 937, 976
BstMWI GCNNNNNNNGC 5 cut(s) 263, 527, 548, 655, 827
BstNSI RCATGY 2 cut(s) 779, 895
BstPI GGTNACC 1 cut(s) 784
BstUI CGCG 2 cut(s) 344, 528
BstV1I GCAGC 2 cut(s) 805, 847
BstV2I GAAGAC 2 cut(s) 161, 592
Bsu15I ATCGAT 1 cut(s) 298
BsuRI GGCC 5 cut(s) 8, 330, 521, 551, 562
BsuTUI ATCGAT 1 cut(s) 298
BtsCI GGATG 6 cut(s) 61, 99, 417, 522, 947, 959
Cac8I GCNNGC 1 cut(s) 893
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 3 cut(s) 344, 530, 728
Cfr10I RCCGGY 1 cut(s) 797
Cfr13I GGNCC 7 cut(s) 329, 380, 386, 466, 519, 549, 561
Cfr9I CCCGGG 1 cut(s) 546
ClaI ATCGAT 1 cut(s) 298
CseI GACGC 1 cut(s) 418
Csp6I GTAC 2 cut(s) 66, 189
CviAII CATG 3 cut(s) 776, 790, 892
CviQI GTAC 2 cut(s) 66, 189
DdeI CTNAG 1 cut(s) 231
DpnI GATC 5 cut(s) 243, 297, 756, 939, 978
DpnII GATC 5 cut(s) 241, 295, 754, 937, 976
Eam1104I CTCTTC 5 cut(s) 83, 106, 123, 584, 958
EarI CTCTTC 5 cut(s) 83, 106, 123, 584, 958
EciI GGCGGA 2 cut(s) 155, 427
Eco130I CCWWGG 1 cut(s) 462
Eco32I GATATC 1 cut(s) 402
Eco47I GGWCC 3 cut(s) 380, 386, 466
Eco57I CTGAAG 2 cut(s) 659, 671
Eco88I CYCGRG 1 cut(s) 546
Eco91I GGTNACC 1 cut(s) 784
EcoNI CCTNNNNNAGG 1 cut(s) 461
EcoO109I RGGNCCY 2 cut(s) 329, 466
EcoO65I GGTNACC 1 cut(s) 784
EcoRV GATATC 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 462
EcoT22I ATGCAT 1 cut(s) 685
ErhI CCWWGG 1 cut(s) 462
Esp3I CGTCTC 1 cut(s) 439
FaeI CATG 3 cut(s) 779, 793, 895
FaiI YATR 8 cut(s) 685, 687, 707, 777, 791, 816, 893, 917
FalI AAGNNNNNCTT 2 cut(s) 120, 152
FaqI GGGAC 2 cut(s) 494, 831
FatI CATG 3 cut(s) 775, 789, 891
FauNDI CATATG 1 cut(s) 685
Fnu4HI GCNGC 3 cut(s) 9, 819, 861
FokI GGATG 6 cut(s) 68, 106, 404, 509, 934, 946
Fsp4HI GCNGC 3 cut(s) 9, 819, 861
FspBI CTAG 1 cut(s) 180
GlaI GCGC 3 cut(s) 343, 529, 727
GluI GCNGC 3 cut(s) 9, 819, 861
GsuI CTGGAG 2 cut(s) 455, 885
HaeII RGCGCY 1 cut(s) 729
HaeIII GGCC 5 cut(s) 8, 330, 521, 551, 562
HgaI GACGC 1 cut(s) 418
HhaI GCGC 3 cut(s) 344, 530, 728
Hin1I GRCGYC 1 cut(s) 410
Hin1II CATG 3 cut(s) 779, 793, 895
Hin6I GCGC 3 cut(s) 342, 528, 726
HinP1I GCGC 3 cut(s) 342, 528, 726
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 3 cut(s) 140, 206, 359
HphI GGTGA 5 cut(s) 97, 262, 409, 595, 796
Hpy166II GTNNAC 3 cut(s) 191, 773, 795
Hpy188I TCNGA 2 cut(s) 673, 827
Hpy188III TCNNGA 3 cut(s) 144, 180, 309
Hpy8I GTNNAC 3 cut(s) 191, 773, 795
Hpy99I CGWCG 3 cut(s) 409, 412, 902
HpyAV CCTTC 5 cut(s) 436, 634, 646, 694, 967
HpyCH4III ACNGT 2 cut(s) 274, 845
HpyCH4V TGCA 3 cut(s) 683, 689, 821
HpyF10VI GCNNNNNNNGC 5 cut(s) 263, 527, 548, 655, 827
HpyF3I CTNAG 1 cut(s) 231
Hsp92I GRCGYC 1 cut(s) 410
Hsp92II CATG 3 cut(s) 779, 793, 895
HspAI GCGC 3 cut(s) 342, 528, 726
Kzo9I GATC 5 cut(s) 241, 295, 754, 937, 976
LguI GCTCTTC 1 cut(s) 584
LmnI GCTCC 3 cut(s) 634, 655, 733
Lsp1109I GCAGC 2 cut(s) 805, 847
MaeI CTAG 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 268, 784
MalI GATC 5 cut(s) 243, 297, 756, 939, 978
MboI GATC 5 cut(s) 241, 295, 754, 937, 976
MboII GAAGA 7 cut(s) 100, 123, 140, 166, 571, 592, 945
MfeI CAATTG 2 cut(s) 566, 758
MluCI AATT 3 cut(s) 566, 758, 918
MlyI GAGTC 3 cut(s) 149, 215, 353
MmeI TCCRAC 2 cut(s) 311, 790
Mph1103I ATGCAT 1 cut(s) 685
MseI TTAA 2 cut(s) 276, 942
MslI CAYNNNNRTG 1 cut(s) 890
MunI CAATTG 2 cut(s) 566, 758
Mva1269I GAATGC 4 cut(s) 685, 821, 862, 870
MvnI CGCG 2 cut(s) 344, 528
MwoI GCNNNNNNNGC 5 cut(s) 263, 527, 548, 655, 827
NdeI CATATG 1 cut(s) 685
NdeII GATC 5 cut(s) 241, 295, 754, 937, 976
NlaIII CATG 3 cut(s) 779, 793, 895
NlaIV GGNNCC 7 cut(s) 331, 369, 382, 388, 563, 589, 735
NmuCI GTSAC 2 cut(s) 268, 784
NsiI ATGCAT 1 cut(s) 685
NspI RCATGY 2 cut(s) 779, 895
PaeI GCATGC 1 cut(s) 895
PciI ACATGT 1 cut(s) 775
PciSI GCTCTTC 1 cut(s) 584
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PctI GAATGC 4 cut(s) 685, 821, 862, 870
PflMI CCANNNNNTGG 1 cut(s) 377
PfoI TCCNGGA 1 cut(s) 322
PkrI GCNGC 3 cut(s) 10, 820, 862
PleI GAGTC 3 cut(s) 148, 214, 353
PpsI GAGTC 3 cut(s) 148, 214, 353
PpuMI RGGWCCY 1 cut(s) 466
PscI ACATGT 1 cut(s) 775
Psp5II RGGWCCY 1 cut(s) 466
PspEI GGTNACC 1 cut(s) 784
PspN4I GGNNCC 7 cut(s) 331, 369, 382, 388, 563, 589, 735
PspPI GGNCC 7 cut(s) 329, 380, 386, 466, 519, 549, 561
PspPPI RGGWCCY 1 cut(s) 466
PstNI CAGNNNCTG 1 cut(s) 377
RsaI GTAC 2 cut(s) 67, 190
RsaNI GTAC 2 cut(s) 66, 189
RseI CAYNNNNRTG 1 cut(s) 890
SapI GCTCTTC 1 cut(s) 584
SaqAI TTAA 2 cut(s) 276, 942
SatI GCNGC 3 cut(s) 9, 819, 861
Sau3AI GATC 5 cut(s) 241, 295, 754, 937, 976
Sau96I GGNCC 7 cut(s) 329, 380, 386, 466, 519, 549, 561
SchI GAGTC 3 cut(s) 149, 215, 353
SinI GGWCC 3 cut(s) 380, 386, 466
SmaI CCCGGG 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 890
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SphI GCATGC 1 cut(s) 895
Sse9I AATT 3 cut(s) 566, 758, 918
SsiI CCGC 5 cut(s) 9, 166, 438, 480, 526
SspMI CTAG 1 cut(s) 180
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 2 cut(s) 274, 845
TaqI TCGA 4 cut(s) 298, 310, 404, 900
TasI AATT 3 cut(s) 566, 758, 918
TatI WGTACW 1 cut(s) 188
TauI GCSGC 1 cut(s) 11
Tru1I TTAA 2 cut(s) 276, 942
Tru9I TTAA 2 cut(s) 276, 942
TseFI GTSAC 2 cut(s) 268, 784
TseI GCWGC 2 cut(s) 818, 860
Tsp45I GTSAC 2 cut(s) 268, 784
TspDTI ATGAA 4 cut(s) 17, 119, 869, 904
TspGWI ACGGA 1 cut(s) 29
TspMI CCCGGG 1 cut(s) 546
Van91I CCANNNNNTGG 1 cut(s) 377
VpaK11BI GGWCC 3 cut(s) 380, 386, 466
XagI CCTNNNNNAGG 1 cut(s) 461
XbaI TCTAGA 1 cut(s) 179
XceI RCATGY 2 cut(s) 779, 895
XmaI CCCGGG 1 cut(s) 546
XspI CTAG 1 cut(s) 180
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.