MD06G1037000.v1.1

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007990
Physical Location & Seq
Forward (+)
4705225 .. 4708485
3261 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1037000.v1.1.491

Sequence Viewer

Length: 1620 bp
ATGGGTAGGAAGAAACCCAAGGACCCAGAAACCGACGCCGTTTCAGCAGCTCAGTCCGACGTCTTCATGACCCTATTCGGCGAGGCCGCCAGCCACGACGCCGCCCCTGCCATCTTCTCCGAGAGCAACCCCTTCAGGAGAAAGAGCCAGGAATCCGGGTTTGGGTTTGTTGCCCCTTCGGCTCAACCTGCTGAGAACACCAATAATGGCGAGTCTGAGAACAATGGCGATGACGGGGACGGCGAGGTGCAGAAACGGAAGCGGAAGAACAAAGAAAAGGCGGCTTCCGGTTTGATTAACGAAGAAGCTCCACAGAGTCATGTGGAGTTGGAGGGCGAAAAACCAAAGAAAAATAAACTGCCGGAAACGCTCAATTCTGAAAACCCTAATTTGGGCTCTGAGTTAGAAGGGGTGCCAAGACAGGAAAATGATGAACCCTCAGAGACGACGAATGCGGTGGCGAAGAAGAAGGATGGGAGGAAGAGGAAAAGGGATGAAGTTGAGAGGGAATATGAGGTGAAGAAGTACGGCGTGAAGGAGGGCGAGGAGGATGGGGACAAGGAGAAGAGGACTGTTGGGGAGAAGAGGAAGACAGTTGATAATCCAGCTGATATGCTGGTTTCCAACGAAGGTTTCGACGACGAGAGTAAGCTCTTGAGGACTGTTTTTGTTGGGAATTTGCCCTTGAAGGTCAAGAAAAAAGCTTTGATGAAGGAGTTCATTAAATTTGGAGAGGTGCAGTCTGTGAGGATTCGATCCGTGCCAATCTTGGATACCAAAAGGCCCAGAAAGGGAGCAATACTCACAAAACAAATCCATGATAAAGCTGACAGCGTTAATGCCTACATTGTTTTCAAAACCGAGGAATCGGCACAGGCTTCTTTGTCCCATAACATGGCTGTGGTTGAAGGACATCATATCCGTGTTGACAGGGCTTGCCCACCTCGTAAGAAGCTGAAAGGGGAGAGTGCTACTGTTTATGATCACACGAGAACAGTTTTTGTGGGTAACCTTCCATTTGATGTAAAGGATGAAGAAGTTTATCAGTTGTTTTGTGGTATCAACAATCTTGGATCCGGCGTTGAAGCTATTCGCATAATCAGGGATCCTAATTATGGTATCGGAAAGGGCATTGCTTATGTTTTGTTTAGAACAAGGGAAACTGCAAATTTGGTCGTTAAGAAGCGAAACTTGAAGCTTGGGGATCGGGAGCTCAGGCTGTCTCATGCAAAACCAGAGTCCACCCCAACTAAGAGAAAGAAACCCTCATCAGCATCGGAAGCTAATTCTTCAGCCAAAAAGCGAGCTGTGGACTCGAGGAGTCCAGATTTTAACAAGTCGAGTTCAAAGGCATCTTACCAGGGCCTGCGTGCAGGTAAGCCTGGTGTCCAAAAGAAGTTCAATGCAAAAGGCAGTAGACCAGATAAGTTTGAATCAAAATCACCAAGTGGAATGAAGCCGAAAGAACGTAAAGATAAACGACCATCCGTTGCTGCGAGAAAGGCTAAAGAAGCACATAAAGGTGGCGCTGCTTCAAAACAAACAGGTACAAAGCGTAAGCTTGATAGCCGGACTCCAGATAGCTCCCAGCAGAAAAAGAAATTCAAGAAATATAGGTAG

Protein Analysis

540

Amino Acids

59.83

Weight (kDa)

9.78

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 333 - 406 5.3e-09 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
Acc36I ACCTGC 2 cut(s) 196, 1364
AccB1I GGYRCC 1 cut(s) 412
AccB7I CCANNNNNTGG 1 cut(s) 895
AccI GTMKAC 1 cut(s) 1417
AciI CCGC 5 cut(s) 87, 102, 262, 281, 455
AclWI GGATC 6 cut(s) 750, 1068, 1081, 1100, 1113, 1212
AcsI RAATTY 4 cut(s) 676, 725, 1168, 1601
AcuI CTGAAG 2 cut(s) 118, 1275
AcyI GRCGYC 3 cut(s) 36, 60, 99
AdeI CACNNNGTG 1 cut(s) 1448
AfaI GTAC 2 cut(s) 527, 1549
AfiI CCNNNNNNNGG 6 cut(s) 162, 391, 392, 791, 895, 1115
AjnI CCWGG 3 cut(s) 147, 1359, 1381
AjuI GAANNNNNNNTTGG 4 cut(s) 144, 176, 617, 649
Alw21I GWGCWC 1 cut(s) 1215
Alw26I GTCTC 2 cut(s) 437, 1227
AlwI GGATC 6 cut(s) 750, 1068, 1081, 1100, 1113, 1212
AlwNI CAGNNNCTG 1 cut(s) 1366
Ama87I CYCGRG 1 cut(s) 1315
AoxI GGCC 3 cut(s) 84, 782, 1363
ApeKI GCWGC 3 cut(s) 47, 1493, 1529
ApoI RAATTY 4 cut(s) 676, 725, 1168, 1601
ArsI GACNNNNNNTTYG 2 cut(s) 1223, 1255
Asp700I GAANNNNTTC 2 cut(s) 716, 1089
AspLEI GCGC 1 cut(s) 1529
AspS9I GGNCC 3 cut(s) 22, 783, 1363
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 2 cut(s) 529, 1434
AvaI CYCGRG 1 cut(s) 1315
AvaII GGWCC 1 cut(s) 22
BamHI GGATCC 2 cut(s) 1073, 1105
BanI GGYRCC 1 cut(s) 412
BanII GRGCYC 2 cut(s) 398, 1215
BauI CACGAG 1 cut(s) 988
BbsI GAAGAC 2 cut(s) 55, 596
Bbv12I GWGCWC 1 cut(s) 1215
BbvI GCAGC 3 cut(s) 59, 1480, 1516
BccI CCATC 4 cut(s) 119, 467, 545, 1492
BceAI ACGGC 3 cut(s) 23, 256, 544
BciT130I CCWGG 3 cut(s) 149, 1361, 1383
BciVI GTATCC 1 cut(s) 766
BclI TGATCA 1 cut(s) 982
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 2 cut(s) 437, 1227
BfoI RGCGCY 1 cut(s) 1530
BfuAI ACCTGC 2 cut(s) 196, 1364
BfuI GTATCC 1 cut(s) 766
BglI GCCNNNNNGGC 1 cut(s) 179
BisI GCNGC 6 cut(s) 48, 87, 102, 282, 1494, 1530
BlsI GCNGC 6 cut(s) 49, 88, 103, 283, 1495, 1531
Bme1390I CCNGG 4 cut(s) 149, 157, 1361, 1383
Bme18I GGWCC 1 cut(s) 22
BmeT110I CYCGRG 1 cut(s) 1315
BmgT120I GGNCC 3 cut(s) 22, 783, 1363
BmiI GGNNCC 4 cut(s) 24, 414, 1075, 1107
BmrFI CCNGG 4 cut(s) 149, 157, 1361, 1383
BmsI GCATC 2 cut(s) 1283, 1361
BpiI GAAGAC 2 cut(s) 55, 596
BplI GAGNNNNNCTC 2 cut(s) 786, 818
BpmI CTGGAG 1 cut(s) 1560
Bpu10I CCTNAGC 1 cut(s) 1214
BpuEI CTTGAG 1 cut(s) 676
BpuMI CCSGG 1 cut(s) 157
BsaHI GRCGYC 3 cut(s) 36, 60, 99
BsaJI CCNNGG 3 cut(s) 18, 861, 1360
BsaWI WCCGGW 1 cut(s) 287
Bsc4I CCNNNNNNNGG 6 cut(s) 162, 391, 392, 791, 895, 1115
Bse3DI GCAATG 1 cut(s) 1131
BseBI CCWGG 3 cut(s) 149, 1361, 1383
BseDI CCNNGG 3 cut(s) 18, 861, 1360
BseGI GGATG 5 cut(s) 478, 499, 556, 1036, 1484
BseLI CCNNNNNNNGG 6 cut(s) 162, 391, 392, 791, 895, 1115
BseMI GCAATG 1 cut(s) 1131
BseMII CTCAG 6 cut(s) 65, 183, 207, 390, 453, 1228
BseRI GAGGAG 2 cut(s) 560, 1333
BseXI GCAGC 3 cut(s) 59, 1480, 1516
BseYI CCCAGC 1 cut(s) 1587
BsgI GTGCAG 3 cut(s) 269, 758, 1392
BshFI GGCC 3 cut(s) 86, 784, 1365
BshNI GGYRCC 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 1215
BsiHKCI CYCGRG 1 cut(s) 1315
BsiSI CCGG 5 cut(s) 156, 288, 362, 1077, 1570
BslFI GGGAC 3 cut(s) 251, 569, 871
BslI CCNNNNNNNGG 6 cut(s) 162, 391, 392, 791, 895, 1115
BsmAI GTCTC 2 cut(s) 437, 1227
BsmBI CGTCTC 1 cut(s) 437
BsmFI GGGAC 3 cut(s) 251, 569, 871
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 3 cut(s) 86, 784, 1365
BsoBI CYCGRG 1 cut(s) 1315
Bsp1286I GDGCHC 2 cut(s) 398, 1215
Bsp143I GATC 5 cut(s) 755, 982, 1073, 1105, 1204
BspACI CCGC 5 cut(s) 87, 102, 262, 281, 455
BspANI GGCC 3 cut(s) 86, 784, 1365
BspCNI CTCAG 6 cut(s) 64, 184, 208, 391, 452, 1227
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 4 cut(s) 24, 414, 1075, 1107
BspMI ACCTGC 2 cut(s) 196, 1364
BspPI GGATC 6 cut(s) 750, 1068, 1081, 1100, 1113, 1212
BspT107I GGYRCC 1 cut(s) 412
BsrDI GCAATG 1 cut(s) 1131
BssECI CCNNGG 3 cut(s) 18, 861, 1360
BssMI GATC 5 cut(s) 755, 982, 1073, 1105, 1204
BssNI GRCGYC 3 cut(s) 36, 60, 99
BssSI CACGAG 1 cut(s) 988
BssT1I CCWWGG 1 cut(s) 18
Bst2BI CACGAG 1 cut(s) 988
Bst2UI CCWGG 3 cut(s) 149, 1361, 1383
Bst4CI ACNGT 5 cut(s) 574, 595, 664, 976, 997
Bst6I CTCTTC 3 cut(s) 476, 560, 578
BstACI GRCGYC 3 cut(s) 36, 60, 99
BstC8I GCNNGC 5 cut(s) 91, 937, 1305, 1367, 1371
BstDEI CTNAG 7 cut(s) 51, 192, 216, 399, 439, 1214, 1251
BstEII GGTNACC 1 cut(s) 1007
BstF5I GGATG 5 cut(s) 478, 499, 556, 1036, 1484
BstH2I RGCGCY 1 cut(s) 1530
BstHHI GCGC 1 cut(s) 1529
BstKTI GATC 5 cut(s) 758, 985, 1076, 1108, 1207
BstMAI GTCTC 2 cut(s) 437, 1227
BstMBI GATC 5 cut(s) 755, 982, 1073, 1105, 1204
BstMWI GCNNNNNNNGC 8 cut(s) 44, 107, 179, 188, 367, 1280, 1502, 1511
BstNI CCWGG 3 cut(s) 149, 1361, 1383
BstPI GGTNACC 1 cut(s) 1007
BstSCI CCNGG 4 cut(s) 147, 155, 1359, 1381
BstV1I GCAGC 3 cut(s) 59, 1480, 1516
BstV2I GAAGAC 2 cut(s) 55, 596
BstX2I RGATCY 2 cut(s) 1073, 1105
BstYI RGATCY 2 cut(s) 1073, 1105
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 3 cut(s) 86, 784, 1365
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 5 cut(s) 478, 499, 556, 1036, 1484
BveI ACCTGC 2 cut(s) 196, 1364
Cac8I GCNNGC 5 cut(s) 91, 937, 1305, 1367, 1371
CaiI CAGNNNCTG 1 cut(s) 1366
CciI TCATGA 1 cut(s) 66
CfoI GCGC 1 cut(s) 1529
Cfr13I GGNCC 3 cut(s) 22, 783, 1363
CseI GACGC 2 cut(s) 44, 107
Csp6I GTAC 2 cut(s) 526, 1548
CviAII CATG 5 cut(s) 67, 320, 818, 895, 1226
CviQI GTAC 2 cut(s) 526, 1548
DdeI CTNAG 7 cut(s) 51, 192, 216, 399, 439, 1214, 1251
DpnI GATC 5 cut(s) 757, 984, 1075, 1107, 1206
DpnII GATC 5 cut(s) 755, 982, 1073, 1105, 1204
DraIII CACNNNGTG 1 cut(s) 1448
Eam1104I CTCTTC 3 cut(s) 476, 560, 578
EarI CTCTTC 3 cut(s) 476, 560, 578
Ecl136II GAGCTC 1 cut(s) 1213
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 2 cut(s) 398, 1215
Eco47I GGWCC 1 cut(s) 22
Eco53kI GAGCTC 1 cut(s) 1213
Eco57I CTGAAG 2 cut(s) 118, 1275
Eco88I CYCGRG 1 cut(s) 1315
Eco91I GGTNACC 1 cut(s) 1007
EcoICRI GAGCTC 1 cut(s) 1213
EcoO109I RGGNCCY 2 cut(s) 22, 1363
EcoO65I GGTNACC 1 cut(s) 1007
EcoRII CCWGG 3 cut(s) 147, 1359, 1381
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 398, 1215
ErhI CCWWGG 1 cut(s) 18
Esp3I CGTCTC 1 cut(s) 437
FaeI CATG 5 cut(s) 70, 323, 821, 898, 1229
FalI AAGNNNNNCTT 2 cut(s) 1175, 1207
FaqI GGGAC 3 cut(s) 251, 569, 871
FatI CATG 5 cut(s) 66, 319, 817, 894, 1225
FbaI TGATCA 1 cut(s) 982
FblI GTMKAC 1 cut(s) 1417
Fnu4HI GCNGC 6 cut(s) 48, 87, 102, 282, 1494, 1530
FokI GGATG 5 cut(s) 485, 506, 563, 1043, 1471
FriOI GRGCYC 2 cut(s) 398, 1215
Fsp4HI GCNGC 6 cut(s) 48, 87, 102, 282, 1494, 1530
GlaI GCGC 1 cut(s) 1528
GluI GCNGC 6 cut(s) 48, 87, 102, 282, 1494, 1530
GsaI CCCAGC 1 cut(s) 1591
GsuI CTGGAG 1 cut(s) 1560
HaeII RGCGCY 1 cut(s) 1530
HaeIII GGCC 3 cut(s) 86, 784, 1365
HapII CCGG 5 cut(s) 156, 288, 362, 1077, 1570
HgaI GACGC 2 cut(s) 44, 107
HhaI GCGC 1 cut(s) 1529
Hin1I GRCGYC 3 cut(s) 36, 60, 99
Hin1II CATG 5 cut(s) 70, 323, 821, 898, 1229
Hin6I GCGC 1 cut(s) 1527
HinP1I GCGC 1 cut(s) 1527
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HindIII AAGCTT 3 cut(s) 702, 1196, 1559
HpaII CCGG 5 cut(s) 156, 288, 362, 1077, 1570
HphI GGTGA 2 cut(s) 529, 1434
Hpy166II GTNNAC 4 cut(s) 928, 1242, 1312, 1418
Hpy188I TCNGA 8 cut(s) 58, 121, 217, 379, 400, 442, 1124, 1279
Hpy188III TCNNGA 8 cut(s) 67, 136, 655, 694, 1208, 1325, 1577, 1606
Hpy8I GTNNAC 4 cut(s) 928, 1242, 1312, 1418
Hpy99I CGWCG 6 cut(s) 38, 62, 101, 451, 641, 644
HpyCH4III ACNGT 5 cut(s) 574, 595, 664, 976, 997
HpyCH4IV ACGT 2 cut(s) 60, 1468
HpyCH4V TGCA 6 cut(s) 250, 739, 1166, 1229, 1373, 1406
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 107, 179, 188, 367, 1280, 1502, 1511
HpyF3I CTNAG 7 cut(s) 51, 192, 216, 399, 439, 1214, 1251
HpySE526I ACGT 2 cut(s) 60, 1468
Hsp92I GRCGYC 3 cut(s) 36, 60, 99
Hsp92II CATG 5 cut(s) 70, 323, 821, 898, 1229
HspAI GCGC 1 cut(s) 1527
Ksp22I TGATCA 1 cut(s) 982
Kzo9I GATC 5 cut(s) 755, 982, 1073, 1105, 1204
LmnI GCTCC 4 cut(s) 313, 794, 1210, 1589
Lsp1109I GCAGC 3 cut(s) 59, 1480, 1516
LweI GCATC 2 cut(s) 1283, 1361
MaeII ACGT 2 cut(s) 60, 1468
MaeIII GTNAC 1 cut(s) 1007
MalI GATC 5 cut(s) 757, 984, 1075, 1107, 1206
MboI GATC 5 cut(s) 755, 982, 1073, 1105, 1204
MflI RGATCY 2 cut(s) 1073, 1105
MhlI GDGCHC 2 cut(s) 398, 1215
MluCI AATT 8 cut(s) 373, 388, 676, 725, 1111, 1168, 1285, 1601
MlyI GAGTC 6 cut(s) 221, 325, 1247, 1307, 1330, 1567
MmeI TCCRAC 3 cut(s) 81, 309, 648
MroXI GAANNNNTTC 2 cut(s) 716, 1089
MseI TTAA 5 cut(s) 297, 723, 837, 1179, 1332
MslI CAYNNNNRTG 3 cut(s) 899, 921, 1521
MspA1I CMGCKG 1 cut(s) 608
MspI CCGG 5 cut(s) 156, 288, 362, 1077, 1570
MspR9I CCNGG 4 cut(s) 149, 157, 1361, 1383
Mva1269I GAATGC 1 cut(s) 457
MvaI CCWGG 3 cut(s) 149, 1361, 1383
MwoI GCNNNNNNNGC 8 cut(s) 44, 107, 179, 188, 367, 1280, 1502, 1511
NciI CCSGG 1 cut(s) 157
NdeII GATC 5 cut(s) 755, 982, 1073, 1105, 1204
NlaIII CATG 5 cut(s) 70, 323, 821, 898, 1229
NlaIV GGNNCC 4 cut(s) 24, 414, 1075, 1107
PaeR7I CTCGAG 1 cut(s) 1315
PagI TCATGA 1 cut(s) 66
PcsI WCGNNNNNNNCGW 2 cut(s) 240, 633
PctI GAATGC 1 cut(s) 457
PdmI GAANNNNTTC 2 cut(s) 716, 1089
PfeI GAWTC 4 cut(s) 152, 751, 866, 1433
PflMI CCANNNNNTGG 1 cut(s) 895
PkrI GCNGC 6 cut(s) 49, 88, 103, 283, 1495, 1531
PleI GAGTC 6 cut(s) 220, 324, 1246, 1307, 1329, 1567
PpsI GAGTC 6 cut(s) 220, 324, 1246, 1307, 1329, 1567
PpuMI RGGWCCY 1 cut(s) 22
Psp124BI GAGCTC 1 cut(s) 1215
Psp5II RGGWCCY 1 cut(s) 22
Psp6I CCWGG 3 cut(s) 147, 1359, 1381
PspEI GGTNACC 1 cut(s) 1007
PspFI CCCAGC 1 cut(s) 1587
PspGI CCWGG 3 cut(s) 147, 1359, 1381
PspN4I GGNNCC 4 cut(s) 24, 414, 1075, 1107
PspPI GGNCC 3 cut(s) 22, 783, 1363
PspPPI RGGWCCY 1 cut(s) 22
PspXI VCTCGAGB 1 cut(s) 1315
PstNI CAGNNNCTG 1 cut(s) 1366
PsuI RGATCY 2 cut(s) 1073, 1105
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 2 cut(s) 527, 1549
RsaNI GTAC 2 cut(s) 526, 1548
RseI CAYNNNNRTG 3 cut(s) 899, 921, 1521
SacI GAGCTC 1 cut(s) 1215
SaqAI TTAA 5 cut(s) 297, 723, 837, 1179, 1332
SatI GCNGC 6 cut(s) 48, 87, 102, 282, 1494, 1530
Sau3AI GATC 5 cut(s) 755, 982, 1073, 1105, 1204
Sau96I GGNCC 3 cut(s) 22, 783, 1363
SchI GAGTC 6 cut(s) 221, 325, 1247, 1307, 1330, 1567
ScrFI CCNGG 4 cut(s) 149, 157, 1361, 1383
SduI GDGCHC 2 cut(s) 398, 1215
SfaNI GCATC 2 cut(s) 1283, 1361
Sfr274I CTCGAG 1 cut(s) 1315
SinI GGWCC 1 cut(s) 22
SlaI CTCGAG 1 cut(s) 1315
SmiMI CAYNNNNRTG 3 cut(s) 899, 921, 1521
SmlI CTYRAG 2 cut(s) 655, 1315
SmoI CTYRAG 2 cut(s) 655, 1315
Sse9I AATT 8 cut(s) 373, 388, 676, 725, 1111, 1168, 1285, 1601
SsiI CCGC 5 cut(s) 87, 102, 262, 281, 455
SstI GAGCTC 1 cut(s) 1215
StyD4I CCNGG 4 cut(s) 147, 155, 1359, 1381
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 5 cut(s) 574, 595, 664, 976, 997
TaiI ACGT 2 cut(s) 63, 1471
TaqI TCGA 4 cut(s) 636, 754, 1316, 1340
TasI AATT 8 cut(s) 373, 388, 676, 725, 1111, 1168, 1285, 1601
TauI GCSGC 3 cut(s) 89, 104, 284
TfiI GAWTC 4 cut(s) 152, 751, 866, 1433
Tru1I TTAA 5 cut(s) 297, 723, 837, 1179, 1332
Tru9I TTAA 5 cut(s) 297, 723, 837, 1179, 1332
TseI GCWGC 3 cut(s) 47, 1493, 1529
TspDTI ATGAA 7 cut(s) 55, 447, 510, 709, 725, 1047, 1469
TspGWI ACGGA 4 cut(s) 271, 748, 911, 1477
Van91I CCANNNNNTGG 1 cut(s) 895
VpaK11BI GGWCC 1 cut(s) 22
XapI RAATTY 4 cut(s) 676, 725, 1168, 1601
XhoI CTCGAG 1 cut(s) 1315
XmiI GTMKAC 1 cut(s) 1417
XmnI GAANNNNTTC 2 cut(s) 716, 1089
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.