RLG00000003055

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007990
Physical Location & Seq
Reverse (-)
43739247 .. 43742068
2822 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003055

Sequence Viewer

Length: 1635 bp
ATGGGAAGGAAGAAACCCAAGGAACCAGAAAGCGACGCCGTTTCGGGTAAGCCCGACATATTCAAGACCCTATTCGGCGACGCCGCCACCCAGGACGCCGCCCCCGCCATCTTCTCCGACGACAACCCCTTCAGGAGGAAGAACCAGGAACCAGGGTCAGGGTTTGCCGCTGCTCCGTCACTCGAAACCGTCGGAAACGTCAACCACGGCGGCTCCGAGGACAAGGACGCTGGCAACGGTGAGGTGAAGAAGAAGAAGAGGAAGAAGAAGGCGATGGATTCGGTTGCTGAAGAAGAAGCTTCAGAGGAGGCTGCATTAGAGAGCAAGGAGCCGAAGAAATCGAAGAAGGAAAAACCCAATTTGGAAAACCCTAATTTGGGTTCTGAAAGCAAAGAAAATGATGTAGTGTCCAATTTGGTTTTGGGATTGGGATCGAATACCGAGAATGATCCGAATTTCGGTCTGAGTGTGGTGAAGAAGAAGAGGAAAAGGGATGAGCTTGAGAGGGAATATGAAGCAAAGAAGTATGGCGTGACAGAGAAACAGGAGGATGGAGATTTGGGGAAGAAAGTTGTGGGGGAAAAGAGGAAGACGGTGGATAATCCAGCTGATATGCTGGTTTCAGAGGAAGGTTTCGACGACGAAAGTAAGCTTCTGAGGACTATTTTCGTTGGGAATTTACCGGTGAAGGTGAAGAAGAAGGTGTTGATCAGGGAGTTCAGTAAGTTTGGAGAGGTGGAGTCTGTGAGGATTCGGTCCGTGCCGATAGAGGATACGAAAGCGCCCAGAAAAGTAGCAGTGCTTACAAAGAAACTCCATAAGAAAGCTGACAGCGTGAATGCATACATCGTTTTCAAGTCAGAGGAATCTGCACAGGCTTCTCTGTCCCATAACATGGCTGTGGTTGAACAACATCACATCCGTGTTGACAGGGCATGCCCACCTCGTAAGAAGCTGAAAGGGGAGAATGCTTCTGTTTATGATCACATGAGAACTGTATTTGTGGGCAACCTTCCCTTTGAAGTAAAGGATGAAGAAGTTTATCAGTTGTTTTGTGGCACTAACAATCAGGATTCCGGAGTTGAAGCCATTCGCATAATCAGGGATTCTAACGTTGGTCTCGGAAAGGGCATTGCTTATGTTCTGTTTAAAACAAAGGAAGCTGCAAATGCAGTCGTTAGGAAACGAAATTTGAAGCTTCGAAATCGGGAACTCAGGCTTTCTCATGCAAAACCAGAGTCCGCCCAATCTAAGAGCAAAAACCAAGATTCCACCCCATCTAAGAGAAGCAATCCATCACCTGCATCAATTACCAAGTCCCCAGCTAAAAGGCTAGCAGTCGGTTCAAGGACTCCAGAGTTTAAGGGAAATTCCAAGGCTACTATGTCCTACCAGGGTGTACGTGCGGGTAAAAATGGTGTCCAAAAGTATCCAAAAGCTAAGTTTGAATCCAGGCCTCAGAGTGGAGTGAAGCCGAAAGAACGTACAGAGAAAAGACCATCTGTTGCTGCCAGAAAAGCTAAGGCGGCATTCCAAGGTGGTGCTGCTTCAAAAGTAGCAGGTGTGAAGCGTAAGATGGATAGTCGAACTCCAGAGAGCTCCCACCAGAAAAAGAAATTCAAGAAATTTAGGTAG

Protein Analysis

545

Amino Acids

60.07

Weight (kDa)

9.85

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 333 - 406 2.6e-10 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1309, 1550
Acc36I ACCTGC 2 cut(s) 1309, 1550
AccB7I CCANNNNNTGG 1 cut(s) 895
AccIII TCCGGA 1 cut(s) 1076
AciI CCGC 8 cut(s) 84, 99, 105, 168, 210, 1242, 1406, 1526
AclI AACGTT 1 cut(s) 1113
AclWI GGATC 2 cut(s) 439, 443
AcsI RAATTY 6 cut(s) 454, 676, 1189, 1369, 1616, 1625
AcuI CTGAAG 3 cut(s) 115, 285, 309
AcyI GRCGYC 3 cut(s) 36, 81, 96
AfaI GTAC 2 cut(s) 1401, 1486
AfiI CCNNNNNNNGG 7 cut(s) 135, 158, 376, 377, 458, 895, 1463
AgeI ACCGGT 1 cut(s) 682
AjnI CCWGG 5 cut(s) 90, 144, 151, 1392, 1451
AleI CACNNNNGTG 1 cut(s) 921
Alw21I GWGCWC 1 cut(s) 1601
Alw26I GTCTC 1 cut(s) 1124
AlwI GGATC 2 cut(s) 439, 443
Aor13HI TCCGGA 1 cut(s) 1076
AoxI GGCC 1 cut(s) 1454
ApeKI GCWGC 5 cut(s) 170, 311, 1163, 1508, 1544
ApoI RAATTY 6 cut(s) 454, 676, 1189, 1369, 1616, 1625
ArsI GACNNNNNNTTYG 2 cut(s) 1223, 1255
AsiGI ACCGGT 1 cut(s) 682
Asp700I GAANNNNTTC 1 cut(s) 1089
AspLEI GCGC 1 cut(s) 784
AspS9I GGNCC 1 cut(s) 756
AsuHPI GGTGA 6 cut(s) 251, 256, 484, 697, 703, 1290
AsuII TTCGAA 1 cut(s) 1201
AsuNHI GCTAGC 1 cut(s) 1333
AvaII GGWCC 1 cut(s) 756
BanII GRGCYC 1 cut(s) 1601
BbsI GAAGAC 1 cut(s) 596
Bbv12I GWGCWC 1 cut(s) 1601
BbvI GCAGC 5 cut(s) 157, 298, 1150, 1495, 1531
BccI CCATC 7 cut(s) 116, 268, 545, 1285, 1303, 1507, 1570
BceAI ACGGC 2 cut(s) 23, 223
BciT130I CCWGG 5 cut(s) 92, 146, 153, 1394, 1453
BciVI GTATCC 2 cut(s) 766, 1440
BclI TGATCA 2 cut(s) 708, 982
BcoDI GTCTC 1 cut(s) 1124
BfaI CTAG 1 cut(s) 1334
BfoI RGCGCY 1 cut(s) 785
BfuAI ACCTGC 2 cut(s) 1309, 1550
BfuI GTATCC 2 cut(s) 766, 1440
Bme1390I CCNGG 5 cut(s) 92, 146, 153, 1394, 1453
Bme18I GGWCC 1 cut(s) 756
BmgT120I GGNCC 1 cut(s) 756
BmiI GGNNCC 4 cut(s) 24, 150, 214, 330
BmrFI CCNGG 5 cut(s) 92, 146, 153, 1394, 1453
BmsI GCATC 1 cut(s) 1313
BmtI GCTAGC 1 cut(s) 1337
BpiI GAAGAC 1 cut(s) 596
BpmI CTGGAG 2 cut(s) 1338, 1575
Bpu10I CCTNAGC 1 cut(s) 1521
Bpu14I TTCGAA 1 cut(s) 1201
BpuEI CTTGAG 1 cut(s) 521
BsaAI YACGTR 1 cut(s) 1403
BsaBI GATNNNNATC 1 cut(s) 430
BsaHI GRCGYC 3 cut(s) 36, 81, 96
BsaI GGTCTC 1 cut(s) 1124
BsaJI CCNNGG 8 cut(s) 18, 90, 152, 205, 216, 1374, 1393, 1534
BsaWI WCCGGW 2 cut(s) 682, 1076
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bsc4I CCNNNNNNNGG 7 cut(s) 135, 158, 376, 377, 458, 895, 1463
Bse118I RCCGGY 1 cut(s) 682
Bse3DI GCAATG 1 cut(s) 1131
Bse8I GATNNNNATC 1 cut(s) 430
BseAI TCCGGA 1 cut(s) 1076
BseBI CCWGG 5 cut(s) 92, 146, 153, 1394, 1453
BseDI CCNNGG 8 cut(s) 18, 90, 152, 205, 216, 1374, 1393, 1534
BseGI GGATG 4 cut(s) 499, 556, 918, 1036
BseJI GATNNNNATC 1 cut(s) 430
BseLI CCNNNNNNNGG 7 cut(s) 135, 158, 376, 377, 458, 895, 1463
BseMI GCAATG 1 cut(s) 1131
BseMII CTCAG 4 cut(s) 455, 647, 1228, 1472
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 5 cut(s) 157, 298, 1150, 1495, 1531
BseYI CCCAGC 1 cut(s) 1321
BsgI GTGCAG 1 cut(s) 855
BshFI GGCC 1 cut(s) 1456
BshTI ACCGGT 1 cut(s) 682
BsiHKAI GWGCWC 1 cut(s) 1601
BsiSI CCGG 2 cut(s) 683, 1077
BslFI GGGAC 2 cut(s) 871, 1303
BslI CCNNNNNNNGG 7 cut(s) 135, 158, 376, 377, 458, 895, 1463
BsmAI GTCTC 1 cut(s) 1124
BsmFI GGGAC 2 cut(s) 871, 1303
BsmI GAATGC 3 cut(s) 844, 973, 1529
BsnI GGCC 1 cut(s) 1456
Bso31I GGTCTC 1 cut(s) 1124
Bsp119I TTCGAA 1 cut(s) 1201
Bsp1286I GDGCHC 1 cut(s) 1601
Bsp13I TCCGGA 1 cut(s) 1076
Bsp143I GATC 4 cut(s) 431, 448, 708, 982
BspACI CCGC 8 cut(s) 84, 99, 105, 168, 210, 1242, 1406, 1526
BspANI GGCC 1 cut(s) 1456
BspCNI CTCAG 4 cut(s) 456, 648, 1227, 1471
BspEI TCCGGA 1 cut(s) 1076
BspLI GGNNCC 4 cut(s) 24, 150, 214, 330
BspMI ACCTGC 2 cut(s) 1309, 1550
BspOI GCTAGC 1 cut(s) 1337
BspPI GGATC 2 cut(s) 439, 443
BspT104I TTCGAA 1 cut(s) 1201
BspTNI GGTCTC 1 cut(s) 1124
BsrDI GCAATG 1 cut(s) 1131
BsrFI RCCGGY 1 cut(s) 682
BssAI RCCGGY 1 cut(s) 682
BssECI CCNNGG 8 cut(s) 18, 90, 152, 205, 216, 1374, 1393, 1534
BssMI GATC 4 cut(s) 431, 448, 708, 982
BssNI GRCGYC 3 cut(s) 36, 81, 96
BssT1I CCWWGG 3 cut(s) 18, 1374, 1534
Bst2UI CCWGG 5 cut(s) 92, 146, 153, 1394, 1453
Bst4CI ACNGT 4 cut(s) 190, 239, 595, 997
Bst6I CTCTTC 2 cut(s) 251, 476
BstACI GRCGYC 3 cut(s) 36, 81, 96
BstBAI YACGTR 1 cut(s) 1403
BstBI TTCGAA 1 cut(s) 1201
BstC8I GCNNGC 3 cut(s) 232, 937, 1335
BstDEI CTNAG 8 cut(s) 464, 656, 1214, 1251, 1281, 1440, 1458, 1521
BstDSI CCRYGG 1 cut(s) 205
BstENI CCTNNNNNAGG 1 cut(s) 133
BstF5I GGATG 4 cut(s) 499, 556, 918, 1036
BstH2I RGCGCY 1 cut(s) 785
BstHHI GCGC 1 cut(s) 784
BstKTI GATC 4 cut(s) 434, 451, 711, 985
BstMAI GTCTC 1 cut(s) 1124
BstMBI GATC 4 cut(s) 431, 448, 708, 982
BstMWI GCNNNNNNNGC 4 cut(s) 104, 1169, 1517, 1526
BstNI CCWGG 5 cut(s) 92, 146, 153, 1394, 1453
BstNSI RCATGY 1 cut(s) 939
BstSCI CCNGG 5 cut(s) 90, 144, 151, 1392, 1451
BstV1I GCAGC 5 cut(s) 157, 298, 1150, 1495, 1531
BstV2I GAAGAC 1 cut(s) 596
BsuI GTATCC 2 cut(s) 766, 1440
BsuRI GGCC 1 cut(s) 1456
BtgI CCRYGG 1 cut(s) 205
BtgZI GCGATG 1 cut(s) 287
BtsCI GGATG 4 cut(s) 499, 556, 918, 1036
BtsI GCAGTG 1 cut(s) 804
BtsIMutI CAGTG 1 cut(s) 804
BveI ACCTGC 2 cut(s) 1309, 1550
Cac8I GCNNGC 3 cut(s) 232, 937, 1335
CfoI GCGC 1 cut(s) 784
Cfr10I RCCGGY 1 cut(s) 682
Cfr13I GGNCC 1 cut(s) 756
CpoI CGGWCCG 1 cut(s) 756
CseI GACGC 4 cut(s) 44, 89, 104, 236
Csp6I GTAC 2 cut(s) 1400, 1485
CspAI ACCGGT 1 cut(s) 682
CspI CGGWCCG 1 cut(s) 756
CviAII CATG 4 cut(s) 895, 936, 988, 1226
CviQI GTAC 2 cut(s) 1400, 1485
DdeI CTNAG 8 cut(s) 464, 656, 1214, 1251, 1281, 1440, 1458, 1521
DpnI GATC 4 cut(s) 433, 450, 710, 984
DpnII GATC 4 cut(s) 431, 448, 708, 982
DraI TTTAAA 1 cut(s) 1150
Eam1104I CTCTTC 2 cut(s) 251, 476
EarI CTCTTC 2 cut(s) 251, 476
EciI GGCGGA 1 cut(s) 1231
Ecl136II GAGCTC 1 cut(s) 1599
Eco130I CCWWGG 3 cut(s) 18, 1374, 1534
Eco147I AGGCCT 1 cut(s) 1456
Eco24I GRGCYC 1 cut(s) 1601
Eco31I GGTCTC 1 cut(s) 1124
Eco47I GGWCC 1 cut(s) 756
Eco53kI GAGCTC 1 cut(s) 1599
Eco57I CTGAAG 3 cut(s) 115, 285, 309
EcoICRI GAGCTC 1 cut(s) 1599
EcoNI CCTNNNNNAGG 1 cut(s) 133
EcoRII CCWGG 5 cut(s) 90, 144, 151, 1392, 1451
EcoT14I CCWWGG 3 cut(s) 18, 1374, 1534
EcoT22I ATGCAT 1 cut(s) 844
EcoT38I GRGCYC 1 cut(s) 1601
ErhI CCWWGG 3 cut(s) 18, 1374, 1534
FaeI CATG 4 cut(s) 898, 939, 991, 1229
FaqI GGGAC 2 cut(s) 871, 1303
FatI CATG 4 cut(s) 894, 935, 987, 1225
FauI CCCGC 2 cut(s) 112, 1399
FbaI TGATCA 2 cut(s) 708, 982
FokI GGATG 4 cut(s) 506, 563, 905, 1043
FriOI GRGCYC 1 cut(s) 1601
FspBI CTAG 1 cut(s) 1334
GlaI GCGC 1 cut(s) 783
GsaI CCCAGC 1 cut(s) 1325
GsuI CTGGAG 2 cut(s) 1338, 1575
HaeII RGCGCY 1 cut(s) 785
HaeIII GGCC 1 cut(s) 1456
HapII CCGG 2 cut(s) 683, 1077
HgaI GACGC 4 cut(s) 44, 89, 104, 236
HhaI GCGC 1 cut(s) 784
Hin1I GRCGYC 3 cut(s) 36, 81, 96
Hin1II CATG 4 cut(s) 898, 939, 991, 1229
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HincII GTYRAC 2 cut(s) 202, 928
HindII GTYRAC 2 cut(s) 202, 928
HindIII AAGCTT 3 cut(s) 297, 650, 1196
HpaII CCGG 2 cut(s) 683, 1077
HphI GGTGA 6 cut(s) 251, 256, 484, 697, 703, 1290
Hpy166II GTNNAC 3 cut(s) 202, 928, 1400
Hpy188III TCNNGA 8 cut(s) 64, 133, 1070, 1077, 1208, 1355, 1592, 1621
Hpy8I GTNNAC 3 cut(s) 202, 928, 1400
Hpy99I CGWCG 6 cut(s) 38, 83, 122, 194, 641, 644
HpyAV CCTTC 7 cut(s) 139, 262, 340, 623, 682, 694, 1022
HpyCH4III ACNGT 4 cut(s) 190, 239, 595, 997
HpyCH4IV ACGT 4 cut(s) 198, 1113, 1402, 1483
HpyCH4V TGCA 7 cut(s) 314, 842, 872, 1166, 1172, 1229, 1304
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 1169, 1517, 1526
HpyF3I CTNAG 8 cut(s) 464, 656, 1214, 1251, 1281, 1440, 1458, 1521
HpySE526I ACGT 4 cut(s) 198, 1113, 1402, 1483
Hsp92I GRCGYC 3 cut(s) 36, 81, 96
Hsp92II CATG 4 cut(s) 898, 939, 991, 1229
HspAI GCGC 1 cut(s) 782
Kpn2I TCCGGA 1 cut(s) 1076
Ksp22I TGATCA 2 cut(s) 708, 982
Kzo9I GATC 4 cut(s) 431, 448, 708, 982
LmnI GCTCC 4 cut(s) 178, 218, 328, 1604
Lsp1109I GCAGC 5 cut(s) 157, 298, 1150, 1495, 1531
LweI GCATC 1 cut(s) 1313
MaeI CTAG 1 cut(s) 1334
MaeII ACGT 4 cut(s) 198, 1113, 1402, 1483
MaeIII GTNAC 2 cut(s) 177, 532
MalI GATC 4 cut(s) 433, 450, 710, 984
MboI GATC 4 cut(s) 431, 448, 708, 982
MhlI GDGCHC 1 cut(s) 1601
MlyI GAGTC 3 cut(s) 749, 1247, 1345
MmeI TCCRAC 2 cut(s) 141, 172
Mph1103I ATGCAT 1 cut(s) 844
MroI TCCGGA 1 cut(s) 1076
MroXI GAANNNNTTC 1 cut(s) 1089
MseI TTAA 2 cut(s) 1149, 1362
MslI CAYNNNNRTG 2 cut(s) 899, 921
MspA1I CMGCKG 2 cut(s) 170, 608
MspI CCGG 2 cut(s) 683, 1077
MspR9I CCNGG 5 cut(s) 92, 146, 153, 1394, 1453
Mva1269I GAATGC 3 cut(s) 844, 973, 1529
MvaI CCWGG 5 cut(s) 92, 146, 153, 1394, 1453
MwoI GCNNNNNNNGC 4 cut(s) 104, 1169, 1517, 1526
NdeII GATC 4 cut(s) 431, 448, 708, 982
NheI GCTAGC 1 cut(s) 1333
NlaIII CATG 4 cut(s) 898, 939, 991, 1229
NlaIV GGNNCC 4 cut(s) 24, 150, 214, 330
NmuCI GTSAC 2 cut(s) 177, 532
NsiI ATGCAT 1 cut(s) 844
NspI RCATGY 1 cut(s) 939
NspV TTCGAA 1 cut(s) 1201
OliI CACNNNNGTG 1 cut(s) 921
PaeI GCATGC 1 cut(s) 939
PaqCI CACCTGC 2 cut(s) 1309, 1550
PceI AGGCCT 1 cut(s) 1456
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PctI GAATGC 3 cut(s) 844, 973, 1529
PdmI GAANNNNTTC 1 cut(s) 1089
PfeI GAWTC 7 cut(s) 278, 751, 866, 1073, 1106, 1268, 1448
PflMI CCANNNNNTGG 1 cut(s) 895
PinAI ACCGGT 1 cut(s) 682
PleI GAGTC 3 cut(s) 748, 1246, 1345
PpsI GAGTC 3 cut(s) 748, 1246, 1345
Ppu21I YACGTR 1 cut(s) 1403
Psp124BI GAGCTC 1 cut(s) 1601
Psp1406I AACGTT 1 cut(s) 1113
Psp6I CCWGG 5 cut(s) 90, 144, 151, 1392, 1451
PspFI CCCAGC 1 cut(s) 1321
PspGI CCWGG 5 cut(s) 90, 144, 151, 1392, 1451
PspN4I GGNNCC 4 cut(s) 24, 150, 214, 330
PspPI GGNCC 1 cut(s) 756
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 2 cut(s) 1401, 1486
RsaNI GTAC 2 cut(s) 1400, 1485
RseI CAYNNNNRTG 2 cut(s) 899, 921
Rsr2I CGGWCCG 1 cut(s) 756
RsrII CGGWCCG 1 cut(s) 756
SacI GAGCTC 1 cut(s) 1601
SaqAI TTAA 2 cut(s) 1149, 1362
Sau3AI GATC 4 cut(s) 431, 448, 708, 982
Sau96I GGNCC 1 cut(s) 756
SchI GAGTC 3 cut(s) 749, 1247, 1345
ScrFI CCNGG 5 cut(s) 92, 146, 153, 1394, 1453
SduI GDGCHC 1 cut(s) 1601
SfaNI GCATC 1 cut(s) 1313
SfuI TTCGAA 1 cut(s) 1201
SinI GGWCC 1 cut(s) 756
SmiMI CAYNNNNRTG 2 cut(s) 899, 921
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
SphI GCATGC 1 cut(s) 939
SseBI AGGCCT 1 cut(s) 1456
SsiI CCGC 8 cut(s) 84, 99, 105, 168, 210, 1242, 1406, 1526
SspMI CTAG 1 cut(s) 1334
SstI GAGCTC 1 cut(s) 1601
StuI AGGCCT 1 cut(s) 1456
StyD4I CCNGG 5 cut(s) 90, 144, 151, 1392, 1451
StyI CCWWGG 3 cut(s) 18, 1374, 1534
TaaI ACNGT 4 cut(s) 190, 239, 595, 997
TaiI ACGT 4 cut(s) 201, 1116, 1405, 1486
TaqI TCGA 6 cut(s) 183, 341, 434, 636, 1201, 1585
TaqII GACCGA 2 cut(s) 449, 744
TauI GCSGC 5 cut(s) 86, 101, 170, 213, 1529
TfiI GAWTC 7 cut(s) 278, 751, 866, 1073, 1106, 1268, 1448
Tru1I TTAA 2 cut(s) 1149, 1362
Tru9I TTAA 2 cut(s) 1149, 1362
TscAI CASTG 1 cut(s) 804
TseFI GTSAC 2 cut(s) 177, 532
TseI GCWGC 5 cut(s) 170, 311, 1163, 1508, 1544
Tsp45I GTSAC 2 cut(s) 177, 532
TspDTI ATGAA 2 cut(s) 528, 1047
TspGWI ACGGA 3 cut(s) 165, 748, 911
TspRI CASTG 1 cut(s) 804
Van91I CCANNNNNTGG 1 cut(s) 895
VpaK11BI GGWCC 1 cut(s) 756
XagI CCTNNNNNAGG 1 cut(s) 133
XapI RAATTY 6 cut(s) 454, 676, 1189, 1369, 1616, 1625
XceI RCATGY 1 cut(s) 939
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XmnI GAANNNNTTC 1 cut(s) 1089
XspI CTAG 1 cut(s) 1334
Zsp2I ATGCAT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.