Rh7AG252900

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0007990
Physical Location & Seq
Forward (+)
25336068 .. 25342976
6909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG252900.1

Sequence Viewer

Length: 1473 bp
ATGTTAATTACATTACAAAAAATTTATGGCTCTAAAATTCATAAAATCACCCTAATATTTTGCCAGATTTTCTTAAAAAACATTGGTCCAATGACAAACACACCAACGTTTTCATCGTTTTCCATCTTTGGGCCTGGAACTAAATCCGACCCGGCCCACTGTCAACGCTTTTCTTCTTTGAAACTCTCTCTCCCTCCTCCTTCAGAAAATAGCTCTAGGGTTTCAACCCGCCACTTCCTCTCTCTCTGCCGGAACAAGCTCACAGCTGCGCTCTCTGAGTCTCCGTTTCTCAAATTGAACCAAACTACAATGGGAAGGAAGAAGCCCAAGGACCCAGAAAGCGACGCCGTTTCGGGTCAACCCGACATATTCAAGACCCTATTCGGCGACGCCGCCACCCAGGACGCCGCCCCCGCCATCTTCTCTGACGACAACCCCTTCAGGAGGAAGAGCCAGGAACCAGGGTCAGGGTTTGCCGCTGCTCCGTCGCTCGAAACCGTCGGAAACGTCGACCACGGCGGCTCCGAGGACAAGGACGCTGGCAATGGTGAGGTGAGGAAGAAGAGGAAGAAGAAGGCGATGGATTCGGCTGCTGAAGAAGAAGAAGCTTCAGAGGAGGCTGCATTAGAGAGCAAGAAGCCGAAGAAATCGAAGAAGGAAAAACCCAATTTGGAAAACCCTAATTTGGGTTCTGAAAGCAAAGAAAATGATGTGGTGTCCAATTTGGTTTTGGGATTGGGATCGAATACGGAGAATGATCCGAATTTGGGTCTGAGTGTGGTGAAGAAGAAGAGGAAAAGGGATGAGCTTGAGAGGGAATACGAAGCAAAGAAGTACGGTGTGACAGAGAACCTGGAGGATGGAGATTTGGGGAAGAAAGTTGTGGGGGAAAAGAGGAAGACGGTGGATAATCCAGCTGAAATGCTGGTTTCAGAGGAAGGTTTCGACGACGAAAGTAAGCTTCTGAGGACTATTTTCGTTGGGAATTTACCGGTGAAGGTGAAGAAGAAGGTGTTGATCAGGGAGTTCAGTAAGTTTGGAGAGGTGGAGTCTGTGAGGATTCGGTCCGTGCCGATAGAGGATACGAAAGCGCCCAGAAAAGTAGCAGTGCTTACAAAGAAACTCCATAAGAAAGCTGACAGCGTGAATGCATACATCGTTTTCAAGTCAGAGGAATCTGCGCAGGCTTCTCTGTCCCATAACATGGCTGTGGTTGAAGAACATCACATCCGTGTTGACAGGGCATGCCCACCTCGTAAGAAGCTGAAAGGGGAGAACGCTTCTGTTTATGATCACACGAGAACTGTATTTGTGGGCAACCTTCCCTTTGAAGTAAAGGATGAAGAAGTTTATCAGTTGTTTTGTGGCACTAACAATCAGGATTCCGGAGTTGAAGCCATTCGCATAATCAGGGATTCTAACGTTGGTCTCGGAAAGGGCATTGCTTATGTTCTGTTTAAAACAAAGGTATAG

Protein Analysis

490

Amino Acids

54.12

Weight (kDa)

9.1

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 436 - 489 4.3e-06 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1182
AccB7I CCANNNNNTGG 1 cut(s) 1204
AccI GTMKAC 1 cut(s) 510
AccIII TCCGGA 1 cut(s) 1385
AciI CCGC 6 cut(s) 229, 393, 408, 414, 477, 519
AclI AACGTT 2 cut(s) 107, 1422
AclWI GGATC 2 cut(s) 748, 752
AcsI RAATTY 4 cut(s) 21, 36, 763, 985
AcuI CTGAAG 4 cut(s) 186, 424, 594, 615
AcyI GRCGYC 3 cut(s) 345, 390, 405
AfaI GTAC 1 cut(s) 836
AfiI CCNNNNNNNGG 7 cut(s) 129, 444, 467, 685, 686, 767, 1204
AgeI ACCGGT 1 cut(s) 991
AgsI TTSAA 8 cut(s) 181, 225, 298, 373, 1165, 1217, 1331, 1394
AjnI CCWGG 5 cut(s) 133, 399, 453, 460, 852
AleI CACNNNNGTG 1 cut(s) 1230
AloI GAACNNNNNNTCC 2 cut(s) 1212, 1244
AluBI AGCT 9 cut(s) 213, 259, 266, 608, 808, 917, 961, 1136, 1264
AluI AGCT 9 cut(s) 213, 259, 266, 608, 808, 917, 961, 1136, 1264
Alw26I GTCTC 2 cut(s) 285, 1433
AlwI GGATC 2 cut(s) 748, 752
Aor13HI TCCGGA 1 cut(s) 1385
AoxI GGCC 2 cut(s) 131, 153
ApeKI GCWGC 4 cut(s) 266, 479, 590, 620
ApoI RAATTY 4 cut(s) 21, 36, 763, 985
AsiGI ACCGGT 1 cut(s) 991
Asp700I GAANNNNTTC 1 cut(s) 1398
AspLEI GCGC 3 cut(s) 271, 1093, 1183
AspS9I GGNCC 5 cut(s) 86, 131, 154, 331, 1065
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 6 cut(s) 40, 560, 565, 793, 1006, 1012
AvaII GGWCC 3 cut(s) 86, 331, 1065
BauI CACGAG 1 cut(s) 1297
BbsI GAAGAC 1 cut(s) 905
BbvI GCAGC 4 cut(s) 253, 466, 577, 607
BccI CCATC 4 cut(s) 131, 425, 574, 854
BceAI ACGGC 2 cut(s) 332, 532
BciT130I CCWGG 5 cut(s) 135, 401, 455, 462, 854
BciVI GTATCC 1 cut(s) 1075
BclI TGATCA 2 cut(s) 1017, 1291
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 2 cut(s) 285, 1433
BfaI CTAG 1 cut(s) 216
BfoI RGCGCY 1 cut(s) 1094
BfuI GTATCC 1 cut(s) 1075
BisI GCNGC 8 cut(s) 267, 393, 408, 477, 480, 520, 591, 621
BlsI GCNGC 8 cut(s) 268, 394, 409, 478, 481, 521, 592, 622
Bme1390I CCNGG 6 cut(s) 135, 152, 401, 455, 462, 854
Bme18I GGWCC 3 cut(s) 86, 331, 1065
BmgT120I GGNCC 5 cut(s) 86, 131, 154, 331, 1065
BmiI GGNNCC 3 cut(s) 333, 459, 523
BmrFI CCNGG 6 cut(s) 135, 152, 401, 455, 462, 854
BpiI GAAGAC 1 cut(s) 905
BpmI CTGGAG 1 cut(s) 875
BpuEI CTTGAG 1 cut(s) 830
BpuMI CCSGG 1 cut(s) 152
BsaBI GATNNNNATC 1 cut(s) 739
BsaHI GRCGYC 3 cut(s) 345, 390, 405
BsaI GGTCTC 1 cut(s) 1433
BsaJI CCNNGG 5 cut(s) 327, 399, 461, 514, 525
BsaWI WCCGGW 2 cut(s) 991, 1385
BsaXI ACNNNNNCTCC 4 cut(s) 174, 204, 506, 536
Bsc4I CCNNNNNNNGG 7 cut(s) 129, 444, 467, 685, 686, 767, 1204
Bse118I RCCGGY 1 cut(s) 991
Bse3DI GCAATG 2 cut(s) 550, 1440
Bse8I GATNNNNATC 1 cut(s) 739
BseAI TCCGGA 1 cut(s) 1385
BseBI CCWGG 5 cut(s) 135, 401, 455, 462, 854
BseDI CCNNGG 5 cut(s) 327, 399, 461, 514, 525
BseGI GGATG 4 cut(s) 808, 865, 1227, 1345
BseJI GATNNNNATC 1 cut(s) 739
BseLI CCNNNNNNNGG 7 cut(s) 129, 444, 467, 685, 686, 767, 1204
BseMI GCAATG 2 cut(s) 550, 1440
BseMII CTCAG 3 cut(s) 267, 764, 956
BseRI GAGGAG 2 cut(s) 186, 629
BseXI GCAGC 4 cut(s) 253, 466, 577, 607
BshFI GGCC 2 cut(s) 133, 155
BshTI ACCGGT 1 cut(s) 991
BsiSI CCGG 4 cut(s) 152, 250, 992, 1386
BslFI GGGAC 1 cut(s) 1180
BslI CCNNNNNNNGG 7 cut(s) 129, 444, 467, 685, 686, 767, 1204
BsmAI GTCTC 2 cut(s) 285, 1433
BsmFI GGGAC 1 cut(s) 1180
BsmI GAATGC 1 cut(s) 1153
BsnI GGCC 2 cut(s) 133, 155
Bso31I GGTCTC 1 cut(s) 1433
Bsp13I TCCGGA 1 cut(s) 1385
Bsp143I GATC 4 cut(s) 740, 757, 1017, 1291
BspACI CCGC 6 cut(s) 229, 393, 408, 414, 477, 519
BspANI GGCC 2 cut(s) 133, 155
BspCNI CTCAG 3 cut(s) 268, 765, 957
BspEI TCCGGA 1 cut(s) 1385
BspLI GGNNCC 3 cut(s) 333, 459, 523
BspPI GGATC 2 cut(s) 748, 752
BspQI GCTCTTC 1 cut(s) 443
BspTNI GGTCTC 1 cut(s) 1433
BsrDI GCAATG 2 cut(s) 550, 1440
BsrFI RCCGGY 1 cut(s) 991
BssAI RCCGGY 1 cut(s) 991
BssECI CCNNGG 5 cut(s) 327, 399, 461, 514, 525
BssMI GATC 4 cut(s) 740, 757, 1017, 1291
BssNI GRCGYC 3 cut(s) 345, 390, 405
BssSI CACGAG 1 cut(s) 1297
BssT1I CCWWGG 1 cut(s) 327
Bst2BI CACGAG 1 cut(s) 1297
Bst2UI CCWGG 5 cut(s) 135, 401, 455, 462, 854
Bst4CI ACNGT 5 cut(s) 161, 499, 839, 904, 1306
Bst6I CTCTTC 3 cut(s) 443, 557, 785
BstACI GRCGYC 3 cut(s) 345, 390, 405
BstC8I GCNNGC 3 cut(s) 541, 1185, 1246
BstDEI CTNAG 3 cut(s) 276, 773, 965
BstDSI CCRYGG 1 cut(s) 514
BstENI CCTNNNNNAGG 1 cut(s) 442
BstF5I GGATG 4 cut(s) 808, 865, 1227, 1345
BstH2I RGCGCY 1 cut(s) 1094
BstHHI GCGC 3 cut(s) 271, 1093, 1183
BstKTI GATC 4 cut(s) 743, 760, 1020, 1294
BstMAI GTCTC 2 cut(s) 285, 1433
BstMBI GATC 4 cut(s) 740, 757, 1017, 1291
BstMWI GCNNNNNNNGC 1 cut(s) 413
BstNI CCWGG 5 cut(s) 135, 401, 455, 462, 854
BstNSI RCATGY 1 cut(s) 1248
BstSCI CCNGG 6 cut(s) 133, 150, 399, 453, 460, 852
BstV1I GCAGC 4 cut(s) 253, 466, 577, 607
BstV2I GAAGAC 1 cut(s) 905
BsuI GTATCC 1 cut(s) 1075
BsuRI GGCC 2 cut(s) 133, 155
BtgI CCRYGG 1 cut(s) 514
BtgZI GCGATG 1 cut(s) 593
BtsCI GGATG 4 cut(s) 808, 865, 1227, 1345
BtsI GCAGTG 1 cut(s) 1113
BtsIMutI CAGTG 2 cut(s) 157, 1113
Cac8I GCNNGC 3 cut(s) 541, 1185, 1246
CfoI GCGC 3 cut(s) 271, 1093, 1183
Cfr10I RCCGGY 1 cut(s) 991
Cfr13I GGNCC 5 cut(s) 86, 131, 154, 331, 1065
CpoI CGGWCCG 1 cut(s) 1065
CseI GACGC 4 cut(s) 353, 398, 413, 545
Csp6I GTAC 1 cut(s) 835
CspAI ACCGGT 1 cut(s) 991
CspI CGGWCCG 1 cut(s) 1065
CviAII CATG 2 cut(s) 1204, 1245
CviQI GTAC 1 cut(s) 835
DdeI CTNAG 3 cut(s) 276, 773, 965
DpnI GATC 4 cut(s) 742, 759, 1019, 1293
DpnII GATC 4 cut(s) 740, 757, 1017, 1291
DraI TTTAAA 1 cut(s) 1459
Eam1104I CTCTTC 3 cut(s) 443, 557, 785
EarI CTCTTC 3 cut(s) 443, 557, 785
Eco130I CCWWGG 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 1433
Eco47I GGWCC 3 cut(s) 86, 331, 1065
Eco57I CTGAAG 4 cut(s) 186, 424, 594, 615
EcoNI CCTNNNNNAGG 1 cut(s) 442
EcoO109I RGGNCCY 1 cut(s) 331
EcoRII CCWGG 5 cut(s) 133, 399, 453, 460, 852
EcoT14I CCWWGG 1 cut(s) 327
EcoT22I ATGCAT 1 cut(s) 1153
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 2 cut(s) 1207, 1248
FaqI GGGAC 1 cut(s) 1180
FatI CATG 2 cut(s) 1203, 1244
FauI CCCGC 2 cut(s) 236, 421
FbaI TGATCA 2 cut(s) 1017, 1291
FblI GTMKAC 1 cut(s) 510
Fnu4HI GCNGC 8 cut(s) 267, 393, 408, 477, 480, 520, 591, 621
FokI GGATG 4 cut(s) 815, 872, 1214, 1352
Fsp4HI GCNGC 8 cut(s) 267, 393, 408, 477, 480, 520, 591, 621
FspBI CTAG 1 cut(s) 216
FspI TGCGCA 1 cut(s) 1182
GlaI GCGC 3 cut(s) 270, 1092, 1182
GluI GCNGC 8 cut(s) 267, 393, 408, 477, 480, 520, 591, 621
GsuI CTGGAG 1 cut(s) 875
HaeII RGCGCY 1 cut(s) 1094
HaeIII GGCC 2 cut(s) 133, 155
HapII CCGG 4 cut(s) 152, 250, 992, 1386
HgaI GACGC 4 cut(s) 353, 398, 413, 545
HhaI GCGC 3 cut(s) 271, 1093, 1183
Hin1I GRCGYC 3 cut(s) 345, 390, 405
Hin1II CATG 2 cut(s) 1207, 1248
Hin6I GCGC 3 cut(s) 269, 1091, 1181
HinP1I GCGC 3 cut(s) 269, 1091, 1181
HincII GTYRAC 4 cut(s) 164, 359, 511, 1237
HindII GTYRAC 4 cut(s) 164, 359, 511, 1237
HindIII AAGCTT 2 cut(s) 606, 959
HinfI GANTC 7 cut(s) 278, 584, 1049, 1060, 1175, 1382, 1415
HpaII CCGG 4 cut(s) 152, 250, 992, 1386
HphI GGTGA 6 cut(s) 40, 560, 565, 793, 1006, 1012
Hpy166II GTNNAC 4 cut(s) 164, 359, 511, 1237
Hpy188III TCNNGA 4 cut(s) 373, 442, 1379, 1386
Hpy8I GTNNAC 4 cut(s) 164, 359, 511, 1237
Hpy99I CGWCG 7 cut(s) 347, 392, 490, 503, 512, 950, 953
HpyAV CCTTC 9 cut(s) 210, 309, 448, 568, 649, 932, 991, 1003, 1331
HpyCH4III ACNGT 5 cut(s) 161, 499, 839, 904, 1306
HpyCH4IV ACGT 3 cut(s) 107, 507, 1422
HpyCH4V TGCA 2 cut(s) 623, 1151
HpyF10VI GCNNNNNNNGC 1 cut(s) 413
HpyF3I CTNAG 3 cut(s) 276, 773, 965
HpySE526I ACGT 3 cut(s) 107, 507, 1422
Hsp92I GRCGYC 3 cut(s) 345, 390, 405
Hsp92II CATG 2 cut(s) 1207, 1248
HspAI GCGC 3 cut(s) 269, 1091, 1181
Kpn2I TCCGGA 1 cut(s) 1385
Ksp22I TGATCA 2 cut(s) 1017, 1291
Kzo9I GATC 4 cut(s) 740, 757, 1017, 1291
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 2 cut(s) 487, 527
Lsp1109I GCAGC 4 cut(s) 253, 466, 577, 607
MaeI CTAG 1 cut(s) 216
MaeII ACGT 3 cut(s) 107, 507, 1422
MaeIII GTNAC 1 cut(s) 841
MalI GATC 4 cut(s) 742, 759, 1019, 1293
MboI GATC 4 cut(s) 740, 757, 1017, 1291
MluCI AATT 9 cut(s) 6, 21, 36, 293, 667, 682, 721, 763, 985
MlyI GAGTC 2 cut(s) 287, 1058
MmeI TCCRAC 2 cut(s) 171, 481
Mph1103I ATGCAT 1 cut(s) 1153
MroI TCCGGA 1 cut(s) 1385
MroXI GAANNNNTTC 1 cut(s) 1398
MseI TTAA 3 cut(s) 5, 74, 1458
MslI CAYNNNNRTG 2 cut(s) 1208, 1230
MspA1I CMGCKG 3 cut(s) 266, 479, 917
MspI CCGG 4 cut(s) 152, 250, 992, 1386
MspR9I CCNGG 6 cut(s) 135, 152, 401, 455, 462, 854
Mva1269I GAATGC 1 cut(s) 1153
MvaI CCWGG 5 cut(s) 135, 401, 455, 462, 854
MwoI GCNNNNNNNGC 1 cut(s) 413
NciI CCSGG 1 cut(s) 152
NdeII GATC 4 cut(s) 740, 757, 1017, 1291
NlaIII CATG 2 cut(s) 1207, 1248
NlaIV GGNNCC 3 cut(s) 333, 459, 523
NmuCI GTSAC 1 cut(s) 841
NsbI TGCGCA 1 cut(s) 1182
NsiI ATGCAT 1 cut(s) 1153
NspI RCATGY 1 cut(s) 1248
OliI CACNNNNGTG 1 cut(s) 1230
PaeI GCATGC 1 cut(s) 1248
PciSI GCTCTTC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 3 cut(s) 113, 507, 522
PctI GAATGC 1 cut(s) 1153
PdmI GAANNNNTTC 1 cut(s) 1398
PfeI GAWTC 5 cut(s) 584, 1060, 1175, 1382, 1415
PflMI CCANNNNNTGG 1 cut(s) 1204
PinAI ACCGGT 1 cut(s) 991
PkrI GCNGC 8 cut(s) 268, 394, 409, 478, 481, 521, 592, 622
PleI GAGTC 2 cut(s) 286, 1057
PpsI GAGTC 2 cut(s) 286, 1057
PpuMI RGGWCCY 1 cut(s) 331
Psp1406I AACGTT 2 cut(s) 107, 1422
Psp5II RGGWCCY 1 cut(s) 331
Psp6I CCWGG 5 cut(s) 133, 399, 453, 460, 852
PspGI CCWGG 5 cut(s) 133, 399, 453, 460, 852
PspN4I GGNNCC 3 cut(s) 333, 459, 523
PspPI GGNCC 5 cut(s) 86, 131, 154, 331, 1065
PspPPI RGGWCCY 1 cut(s) 331
PvuII CAGCTG 2 cut(s) 266, 917
RsaI GTAC 1 cut(s) 836
RsaNI GTAC 1 cut(s) 835
RseI CAYNNNNRTG 2 cut(s) 1208, 1230
Rsr2I CGGWCCG 1 cut(s) 1065
RsrII CGGWCCG 1 cut(s) 1065
SalI GTCGAC 1 cut(s) 509
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 3 cut(s) 5, 74, 1458
SatI GCNGC 8 cut(s) 267, 393, 408, 477, 480, 520, 591, 621
Sau3AI GATC 4 cut(s) 740, 757, 1017, 1291
Sau96I GGNCC 5 cut(s) 86, 131, 154, 331, 1065
SchI GAGTC 2 cut(s) 287, 1058
ScrFI CCNGG 6 cut(s) 135, 152, 401, 455, 462, 854
SinI GGWCC 3 cut(s) 86, 331, 1065
SmiMI CAYNNNNRTG 2 cut(s) 1208, 1230
SmlI CTYRAG 1 cut(s) 809
SmoI CTYRAG 1 cut(s) 809
SphI GCATGC 1 cut(s) 1248
Sse9I AATT 9 cut(s) 6, 21, 36, 293, 667, 682, 721, 763, 985
SsiI CCGC 6 cut(s) 229, 393, 408, 414, 477, 519
SspI AATATT 1 cut(s) 57
SspMI CTAG 1 cut(s) 216
StyD4I CCNGG 6 cut(s) 133, 150, 399, 453, 460, 852
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 5 cut(s) 161, 499, 839, 904, 1306
TaiI ACGT 3 cut(s) 110, 510, 1425
TaqI TCGA 5 cut(s) 492, 510, 650, 743, 945
TaqII GACCGA 1 cut(s) 1053
TasI AATT 9 cut(s) 6, 21, 36, 293, 667, 682, 721, 763, 985
TauI GCSGC 4 cut(s) 395, 410, 479, 522
TfiI GAWTC 5 cut(s) 584, 1060, 1175, 1382, 1415
Tru1I TTAA 3 cut(s) 5, 74, 1458
Tru9I TTAA 3 cut(s) 5, 74, 1458
TscAI CASTG 2 cut(s) 164, 1113
TseFI GTSAC 1 cut(s) 841
TseI GCWGC 4 cut(s) 266, 479, 590, 620
Tsp45I GTSAC 1 cut(s) 841
TspDTI ATGAA 3 cut(s) 29, 102, 1356
TspGWI ACGGA 5 cut(s) 273, 474, 764, 1057, 1220
TspRI CASTG 2 cut(s) 164, 1113
Van91I CCANNNNNTGG 1 cut(s) 1204
VpaK11BI GGWCC 3 cut(s) 86, 331, 1065
XagI CCTNNNNNAGG 1 cut(s) 442
XapI RAATTY 4 cut(s) 21, 36, 763, 985
XceI RCATGY 1 cut(s) 1248
XcmI CCANNNNNNNNNTGG 1 cut(s) 727
XmiI GTMKAC 1 cut(s) 510
XmnI GAANNNNTTC 1 cut(s) 1398
XspI CTAG 1 cut(s) 216
Zsp2I ATGCAT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.