MD16G1272400.v1.1

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007990
Physical Location & Seq
Reverse (-)
35455339 .. 35458766
3428 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1272400.v1.1.491

Sequence Viewer

Length: 1623 bp
ATGGGTAGGAAGAAACCCAAGGAGCCAGAAAGCGACGCCGTTTCAGCAGCTCAGTCCGACGTCTTCAAGACCCTGTTCGGCGACTCCGTCAGCCACGACGCCGCCCCTGCCATCTTCTCTGACAGCAACCCCTTCAGGAGGAAGAACAAGGAACCCGACCAAGGGTTTGTTGCCCCTTCGGCTGAACCTGCTGAAAACATCAATTATGTCGAGCCTGAAAACAATGGCGATGACGGTGACGGTGACGTGAAGAAGCGGAAGAGGAGGAGCAAAGAGAAAGCGGCTTCCGGTTCGCTTAACGAAGAAGTTTCAGAGGGTCCTGTGGAGTTGGAGGGCAGAAAAACGAAGAAAAAGAAGCTGCTGGAAACGCCCGATTCTGAAAACCCTAATTTGGGTTCTGAGTTTGAAGGGGTGTCAAGACAGGAAAGTGATGAAGCCTCGGAGAAAGGGAATGCCCCGGCGAAGAAGAAGGATGGGAAGAAGAGGAAAAGGGATGAGGTTGAGAGGGAATATGAGGTGAAGAAGTATGGGGTGAAGGAGGGTGAGGAGGATGGGGACAAGGAGGAGAAGAGGACTGTTGGGGAGAAGAGGAAGACAGTGGATAACCCAGCTGATATGTTGGTTTCGACCGAAGGTTTTGATGACGAGAGCAAGCTATTGAGGACTGTTTTTGTTGGAAATTTGCCCTTGAAGGTCAAGAAGAAGGCTTTGATGAAGGAGTTCACTAAGTTTGGAGAGGTGGAGTCGGTGAGGATTCGCTCCGTGCCAATCTTGGATACGAAAAGACCCAGAAAGGGAGCAATACTCACCAAGCAAATCCACGATAAAGCTGACAGCGTTAATGCCTACGTTGTTTTCAAAACGGAGGAATCGGCACAGACTTCCTTGTCCAATAACATGGCTGTGGTTGAAGGACATCATATCCGTGTTGACAGGGCATGCCCACCTCGTAAGAAGCTGAAAGGGGAGAGTGCTACTGTTTACGATCACACGAGAACTGTTTTTGTGGGTAACCTTCCATTTGATGTGAAGGATGAAGAAGTTTATCAGTTGTTTTGTGGTATCAACAATCTCGGTTCCAGTGTTGAAGCTATTCGCATAATCAGGGATCCTAACTATGGTATCGGAAAGGGCATTGCTTATGTTTTGTTTAGAACAAGGGAAGCTGCAAATTTGGTTGTTAAGAGGCGAAACTTGAAGCTTGGGGATCGGGAGCTCAGGCTGTCTCATGCAAAACCAGAGTCAACCCCAACGAAGAGAAAGAACCCCTCATCTGCACCAGAAGCTAAGTCTTCAGCCAAAAAGCGAGCTGTGGACTCAAGGAGTCCAGATTTTAACAAGTCTAGTTCAAAAGCATCTTACCAGGGCCTGCGTGCTGGTAAGCCTGGTGTCCAAAAGAAGTTTAATGCAAAAGGCAGTAGACCAGATAAGTTTGAATCAAGATCCCCTAGTGGAGTGAAGCCGAAAGAGCGTAAAGATAAAAGACCTTCTGTTGCTGCGAGAAAGGCTAAAGAAGTACTGAAAGGTGGTGCTGCATCAAAACAAACAGGTACAAAGCGTAAGCTGGATAGCCGGAGTCCAGATAGCTCCCAGCAGAAAAAGAAATTCAAGAAATTTAGGTAG

Protein Analysis

541

Amino Acids

60.02

Weight (kDa)

9.77

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 334 - 406 7.5e-09 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 886
AatII GACGTC 1 cut(s) 63
Acc36I ACCTGC 1 cut(s) 196
AccI GTMKAC 1 cut(s) 1420
AciI CCGC 3 cut(s) 102, 256, 281
AclWI GGATC 4 cut(s) 1103, 1116, 1215, 1437
AcsI RAATTY 4 cut(s) 679, 1171, 1604, 1613
AcuI CTGAAG 2 cut(s) 118, 1278
AcyI GRCGYC 3 cut(s) 36, 60, 99
AfaI GTAC 2 cut(s) 1518, 1552
AfiI CCNNNNNNNGG 7 cut(s) 138, 161, 162, 391, 392, 794, 1118
AjiI CACGTC 1 cut(s) 247
AjnI CCWGG 2 cut(s) 1362, 1384
Alw21I GWGCWC 1 cut(s) 1218
Alw26I GTCTC 1 cut(s) 1230
AlwI GGATC 4 cut(s) 1103, 1116, 1215, 1437
AlwNI CAGNNNCTG 1 cut(s) 1369
AoxI GGCC 1 cut(s) 1366
ApeKI GCWGC 5 cut(s) 47, 358, 1166, 1496, 1532
ApoI RAATTY 4 cut(s) 679, 1171, 1604, 1613
ArsI GACNNNNNNTTYG 4 cut(s) 149, 181, 1226, 1258
Asp700I GAANNNNTTC 2 cut(s) 719, 1092
AspS9I GGNCC 2 cut(s) 317, 1366
AsuC2I CCSGG 1 cut(s) 458
AsuHPI GGTGA 7 cut(s) 248, 254, 529, 544, 554, 760, 799
AvaII GGWCC 1 cut(s) 317
BaeI ACNNNNGTAYC 2 cut(s) 1105, 1138
BamHI GGATCC 1 cut(s) 1108
BanII GRGCYC 1 cut(s) 1218
BauI CACGAG 1 cut(s) 991
BbsI GAAGAC 3 cut(s) 55, 599, 1284
Bbv12I GWGCWC 1 cut(s) 1218
BbvI GCAGC 5 cut(s) 59, 345, 1153, 1483, 1519
BccI CCATC 3 cut(s) 119, 467, 545
BceAI ACGGC 1 cut(s) 23
BciT130I CCWGG 2 cut(s) 1364, 1386
BciVI GTATCC 1 cut(s) 769
BcnI CCSGG 1 cut(s) 458
BcoDI GTCTC 1 cut(s) 1230
BfaI CTAG 2 cut(s) 1344, 1449
BfuAI ACCTGC 1 cut(s) 196
BfuI GTATCC 1 cut(s) 769
BglI GCCNNNNNGGC 1 cut(s) 179
BisI GCNGC 7 cut(s) 48, 102, 282, 359, 1167, 1497, 1533
BlsI GCNGC 7 cut(s) 49, 103, 283, 360, 1168, 1498, 1534
BmcAI AGTACT 1 cut(s) 1518
Bme1390I CCNGG 3 cut(s) 458, 1364, 1386
Bme18I GGWCC 1 cut(s) 317
BmgBI CACGTC 1 cut(s) 247
BmgT120I GGNCC 2 cut(s) 317, 1366
BmiI GGNNCC 5 cut(s) 24, 153, 318, 1078, 1110
BmrFI CCNGG 3 cut(s) 458, 1364, 1386
BmsI GCATC 2 cut(s) 1364, 1544
BpiI GAAGAC 3 cut(s) 55, 599, 1284
BplI GAGNNNNNCTC 2 cut(s) 789, 821
Bpu10I CCTNAGC 1 cut(s) 1217
BpuEI CTTGAG 1 cut(s) 1303
BpuMI CCSGG 1 cut(s) 458
BsaHI GRCGYC 3 cut(s) 36, 60, 99
BsaJI CCNNGG 5 cut(s) 18, 160, 438, 456, 1363
BsaWI WCCGGW 1 cut(s) 287
Bsc4I CCNNNNNNNGG 7 cut(s) 138, 161, 162, 391, 392, 794, 1118
Bse1I ACTGG 1 cut(s) 1080
Bse3DI GCAATG 1 cut(s) 1134
BseBI CCWGG 2 cut(s) 1364, 1386
BseDI CCNNGG 5 cut(s) 18, 160, 438, 456, 1363
BseGI GGATG 4 cut(s) 478, 499, 556, 1039
BseLI CCNNNNNNNGG 7 cut(s) 138, 161, 162, 391, 392, 794, 1118
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 3 cut(s) 65, 390, 1231
BseNI ACTGG 1 cut(s) 1080
BseRI GAGGAG 4 cut(s) 277, 280, 560, 578
BseXI GCAGC 5 cut(s) 59, 345, 1153, 1483, 1519
BseYI CCCAGC 2 cut(s) 607, 1590
BsgI GTGCAG 1 cut(s) 1260
Bsh1285I CGRYCG 1 cut(s) 630
BshFI GGCC 1 cut(s) 1368
BsiEI CGRYCG 1 cut(s) 630
BsiHKAI GWGCWC 1 cut(s) 1218
BsiSI CCGG 3 cut(s) 288, 458, 1573
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 7 cut(s) 138, 161, 162, 391, 392, 794, 1118
BsmAI GTCTC 1 cut(s) 1230
BsmFI GGGAC 1 cut(s) 569
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 1 cut(s) 1368
Bsp1286I GDGCHC 1 cut(s) 1218
Bsp143I GATC 4 cut(s) 985, 1108, 1207, 1442
BspACI CCGC 3 cut(s) 102, 256, 281
BspANI GGCC 1 cut(s) 1368
BspCNI CTCAG 3 cut(s) 64, 391, 1230
BspLI GGNNCC 5 cut(s) 24, 153, 318, 1078, 1110
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 4 cut(s) 1103, 1116, 1215, 1437
BsrDI GCAATG 1 cut(s) 1134
BsrI ACTGG 1 cut(s) 1080
BssECI CCNNGG 5 cut(s) 18, 160, 438, 456, 1363
BssMI GATC 4 cut(s) 985, 1108, 1207, 1442
BssNI GRCGYC 3 cut(s) 36, 60, 99
BssSI CACGAG 1 cut(s) 991
BssT1I CCWWGG 2 cut(s) 18, 160
Bst2BI CACGAG 1 cut(s) 991
Bst2UI CCWGG 2 cut(s) 1364, 1386
Bst4CI ACNGT 7 cut(s) 236, 242, 577, 598, 667, 979, 1000
Bst6I CTCTTC 5 cut(s) 254, 476, 563, 581, 1250
BstACI GRCGYC 3 cut(s) 36, 60, 99
BstC8I GCNNGC 5 cut(s) 653, 940, 1308, 1370, 1374
BstDEI CTNAG 5 cut(s) 51, 399, 726, 1217, 1287
BstEII GGTNACC 1 cut(s) 1010
BstENI CCTNNNNNAGG 1 cut(s) 136
BstF5I GGATG 4 cut(s) 478, 499, 556, 1039
BstKTI GATC 4 cut(s) 988, 1111, 1210, 1445
BstMAI GTCTC 1 cut(s) 1230
BstMBI GATC 4 cut(s) 985, 1108, 1207, 1442
BstMCI CGRYCG 1 cut(s) 630
BstMWI GCNNNNNNNGC 8 cut(s) 44, 107, 179, 188, 367, 1283, 1468, 1505
BstNI CCWGG 2 cut(s) 1364, 1386
BstNSI RCATGY 1 cut(s) 942
BstPI GGTNACC 1 cut(s) 1010
BstSCI CCNGG 3 cut(s) 456, 1362, 1384
BstV1I GCAGC 5 cut(s) 59, 345, 1153, 1483, 1519
BstV2I GAAGAC 3 cut(s) 55, 599, 1284
BstX2I RGATCY 2 cut(s) 1108, 1442
BstXI CCANNNNNNTGG 1 cut(s) 898
BstYI RGATCY 2 cut(s) 1108, 1442
BsuI GTATCC 1 cut(s) 769
BsuRI GGCC 1 cut(s) 1368
BtgZI GCGATG 1 cut(s) 243
BtrI CACGTC 1 cut(s) 247
BtsCI GGATG 4 cut(s) 478, 499, 556, 1039
BtsIMutI CAGTG 2 cut(s) 603, 1087
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 5 cut(s) 653, 940, 1308, 1370, 1374
CaiI CAGNNNCTG 1 cut(s) 1369
Cfr13I GGNCC 2 cut(s) 317, 1366
CseI GACGC 2 cut(s) 44, 107
Csp6I GTAC 2 cut(s) 1517, 1551
CviAII CATG 3 cut(s) 898, 939, 1229
CviQI GTAC 2 cut(s) 1517, 1551
DdeI CTNAG 5 cut(s) 51, 399, 726, 1217, 1287
DpnI GATC 4 cut(s) 987, 1110, 1209, 1444
DpnII GATC 4 cut(s) 985, 1108, 1207, 1442
DrdI GACNNNNNNGTC 1 cut(s) 886
DseDI GACNNNNNNGTC 1 cut(s) 886
Eam1104I CTCTTC 5 cut(s) 254, 476, 563, 581, 1250
EarI CTCTTC 5 cut(s) 254, 476, 563, 581, 1250
Ecl136II GAGCTC 1 cut(s) 1216
Eco130I CCWWGG 2 cut(s) 18, 160
Eco24I GRGCYC 1 cut(s) 1218
Eco47I GGWCC 1 cut(s) 317
Eco53kI GAGCTC 1 cut(s) 1216
Eco57I CTGAAG 2 cut(s) 118, 1278
Eco91I GGTNACC 1 cut(s) 1010
EcoICRI GAGCTC 1 cut(s) 1216
EcoNI CCTNNNNNAGG 1 cut(s) 136
EcoO109I RGGNCCY 2 cut(s) 317, 1366
EcoO65I GGTNACC 1 cut(s) 1010
EcoRII CCWGG 2 cut(s) 1362, 1384
EcoT14I CCWWGG 2 cut(s) 18, 160
EcoT38I GRGCYC 1 cut(s) 1218
ErhI CCWWGG 2 cut(s) 18, 160
FaeI CATG 3 cut(s) 901, 942, 1232
FaqI GGGAC 1 cut(s) 569
FatI CATG 3 cut(s) 897, 938, 1228
FblI GTMKAC 1 cut(s) 1420
Fnu4HI GCNGC 7 cut(s) 48, 102, 282, 359, 1167, 1497, 1533
FokI GGATG 4 cut(s) 485, 506, 563, 1046
FriOI GRGCYC 1 cut(s) 1218
Fsp4HI GCNGC 7 cut(s) 48, 102, 282, 359, 1167, 1497, 1533
FspBI CTAG 2 cut(s) 1344, 1449
GluI GCNGC 7 cut(s) 48, 102, 282, 359, 1167, 1497, 1533
GsaI CCCAGC 2 cut(s) 611, 1594
HaeIII GGCC 1 cut(s) 1368
HapII CCGG 3 cut(s) 288, 458, 1573
HgaI GACGC 2 cut(s) 44, 107
Hin1I GRCGYC 3 cut(s) 36, 60, 99
Hin1II CATG 3 cut(s) 901, 942, 1232
HincII GTYRAC 2 cut(s) 931, 1245
HindII GTYRAC 2 cut(s) 931, 1245
HindIII AAGCTT 1 cut(s) 1199
HpaII CCGG 3 cut(s) 288, 458, 1573
HphI GGTGA 7 cut(s) 248, 254, 529, 544, 554, 760, 799
Hpy166II GTNNAC 6 cut(s) 723, 931, 982, 1245, 1315, 1421
Hpy188I TCNGA 7 cut(s) 58, 121, 313, 379, 400, 442, 1127
Hpy188III TCNNGA 9 cut(s) 67, 136, 417, 697, 1211, 1328, 1440, 1580, 1609
Hpy8I GTNNAC 6 cut(s) 723, 931, 982, 1245, 1315, 1421
Hpy99I CGWCG 3 cut(s) 38, 62, 101
HpyCH4III ACNGT 7 cut(s) 236, 242, 577, 598, 667, 979, 1000
HpyCH4IV ACGT 3 cut(s) 60, 246, 849
HpyCH4V TGCA 5 cut(s) 1169, 1232, 1277, 1409, 1535
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 107, 179, 188, 367, 1283, 1468, 1505
HpyF3I CTNAG 5 cut(s) 51, 399, 726, 1217, 1287
HpySE526I ACGT 3 cut(s) 60, 246, 849
Hsp92I GRCGYC 3 cut(s) 36, 60, 99
Hsp92II CATG 3 cut(s) 901, 942, 1232
Kzo9I GATC 4 cut(s) 985, 1108, 1207, 1442
LmnI GCTCC 6 cut(s) 22, 267, 764, 797, 1213, 1592
Lsp1109I GCAGC 5 cut(s) 59, 345, 1153, 1483, 1519
LweI GCATC 2 cut(s) 1364, 1544
MaeI CTAG 2 cut(s) 1344, 1449
MaeII ACGT 3 cut(s) 60, 246, 849
MaeIII GTNAC 3 cut(s) 236, 242, 1010
MalI GATC 4 cut(s) 987, 1110, 1209, 1444
MboI GATC 4 cut(s) 985, 1108, 1207, 1442
MflI RGATCY 2 cut(s) 1108, 1442
MhlI GDGCHC 1 cut(s) 1218
MluCI AATT 6 cut(s) 202, 388, 679, 1171, 1604, 1613
MlyI GAGTC 6 cut(s) 77, 752, 1250, 1310, 1333, 1585
MmeI TCCRAC 3 cut(s) 81, 309, 655
MroXI GAANNNNTTC 2 cut(s) 719, 1092
MseI TTAA 5 cut(s) 297, 840, 1182, 1335, 1404
MslI CAYNNNNRTG 2 cut(s) 902, 924
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 3 cut(s) 288, 458, 1573
MspR9I CCNGG 3 cut(s) 458, 1364, 1386
Mva1269I GAATGC 1 cut(s) 457
MvaI CCWGG 2 cut(s) 1364, 1386
MwoI GCNNNNNNNGC 8 cut(s) 44, 107, 179, 188, 367, 1283, 1468, 1505
NciI CCSGG 1 cut(s) 458
NdeII GATC 4 cut(s) 985, 1108, 1207, 1442
NlaIII CATG 3 cut(s) 901, 942, 1232
NlaIV GGNNCC 5 cut(s) 24, 153, 318, 1078, 1110
NmuCI GTSAC 2 cut(s) 236, 242
NspI RCATGY 1 cut(s) 942
PaeI GCATGC 1 cut(s) 942
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 1 cut(s) 457
PdmI GAANNNNTTC 2 cut(s) 719, 1092
PfeI GAWTC 4 cut(s) 374, 754, 869, 1436
PflFI GACNNNGTC 1 cut(s) 86
PkrI GCNGC 7 cut(s) 49, 103, 283, 360, 1168, 1498, 1534
PleI GAGTC 6 cut(s) 77, 751, 1249, 1310, 1332, 1584
PpsI GAGTC 6 cut(s) 77, 751, 1249, 1310, 1332, 1584
PpuMI RGGWCCY 1 cut(s) 317
Psp124BI GAGCTC 1 cut(s) 1218
Psp5II RGGWCCY 1 cut(s) 317
Psp6I CCWGG 2 cut(s) 1362, 1384
PspEI GGTNACC 1 cut(s) 1010
PspFI CCCAGC 2 cut(s) 607, 1590
PspGI CCWGG 2 cut(s) 1362, 1384
PspN4I GGNNCC 5 cut(s) 24, 153, 318, 1078, 1110
PspPI GGNCC 2 cut(s) 317, 1366
PspPPI RGGWCCY 1 cut(s) 317
PstNI CAGNNNCTG 1 cut(s) 1369
PsuI RGATCY 2 cut(s) 1108, 1442
PsyI GACNNNGTC 1 cut(s) 86
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 2 cut(s) 1518, 1552
RsaNI GTAC 2 cut(s) 1517, 1551
RseI CAYNNNNRTG 2 cut(s) 902, 924
SacI GAGCTC 1 cut(s) 1218
SaqAI TTAA 5 cut(s) 297, 840, 1182, 1335, 1404
SatI GCNGC 7 cut(s) 48, 102, 282, 359, 1167, 1497, 1533
Sau3AI GATC 4 cut(s) 985, 1108, 1207, 1442
Sau96I GGNCC 2 cut(s) 317, 1366
ScaI AGTACT 1 cut(s) 1518
SchI GAGTC 6 cut(s) 77, 752, 1250, 1310, 1333, 1585
ScrFI CCNGG 3 cut(s) 458, 1364, 1386
SduI GDGCHC 1 cut(s) 1218
SfaNI GCATC 2 cut(s) 1364, 1544
SinI GGWCC 1 cut(s) 317
SmiMI CAYNNNNRTG 2 cut(s) 902, 924
SmlI CTYRAG 1 cut(s) 1318
SmoI CTYRAG 1 cut(s) 1318
SphI GCATGC 1 cut(s) 942
Sse9I AATT 6 cut(s) 202, 388, 679, 1171, 1604, 1613
SsiI CCGC 3 cut(s) 102, 256, 281
SspMI CTAG 2 cut(s) 1344, 1449
SstI GAGCTC 1 cut(s) 1218
StyD4I CCNGG 3 cut(s) 456, 1362, 1384
StyI CCWWGG 2 cut(s) 18, 160
TaaI ACNGT 7 cut(s) 236, 242, 577, 598, 667, 979, 1000
TaiI ACGT 3 cut(s) 63, 249, 852
TaqI TCGA 2 cut(s) 210, 626
TaqII GACCGA 1 cut(s) 644
TasI AATT 6 cut(s) 202, 388, 679, 1171, 1604, 1613
TatI WGTACW 1 cut(s) 1516
TauI GCSGC 2 cut(s) 104, 284
TfiI GAWTC 4 cut(s) 374, 754, 869, 1436
Tru1I TTAA 5 cut(s) 297, 840, 1182, 1335, 1404
Tru9I TTAA 5 cut(s) 297, 840, 1182, 1335, 1404
TscAI CASTG 2 cut(s) 603, 1087
TseFI GTSAC 2 cut(s) 236, 242
TseI GCWGC 5 cut(s) 47, 358, 1166, 1496, 1532
Tsp45I GTSAC 2 cut(s) 236, 242
TspDTI ATGAA 3 cut(s) 447, 728, 1050
TspGWI ACGGA 4 cut(s) 76, 751, 878, 914
TspRI CASTG 2 cut(s) 603, 1087
Tth111I GACNNNGTC 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 317
XagI CCTNNNNNAGG 1 cut(s) 136
XapI RAATTY 4 cut(s) 679, 1171, 1604, 1613
XceI RCATGY 1 cut(s) 942
XmiI GTMKAC 1 cut(s) 1420
XmnI GAANNNNTTC 2 cut(s) 719, 1092
XspI CTAG 2 cut(s) 1344, 1449
ZraI GACGTC 1 cut(s) 61
ZrmI AGTACT 1 cut(s) 1518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.