MD06G1056700.v1.1

Formyltetrahydrofolate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002503
Physical Location & Seq
Forward (+)
8536287 .. 8541460
5174 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1056700.v1.1.491

Sequence Viewer

Length: 855 bp
ATGAACCTCGTCCAAAGGGCATCTCCAAGCCTCCCCAAGCTTTTCGGACTTGCCAAGAGGCCCTTCAAGTCCTTGAGATTCCCAGGGGAGCCCCTCGACTCTTCATCTTCTCTCCCCCCTCTCTCCTACGGAATCCACGTCTTCCATTGCCCCGATGCAGTTGGGATTGTGGCCAAGCTATCGGATTGCATTGCTTCTAAAGGCGGAAACATTCTCGGCGCCAGCGTCTTCGTGCCCGAAAACAAGCACGTTTTTTATTCCAGAAGCGAATTTATTTTCGATCCTCTTAAATGGCCAAGAAAGGAAATGGATGAGGATTTCCATAAGCTGTCGTTAACATACTCTGCAATCAGATCTGTTGTCCGGGTTCCAAATCTCGACCCCAAATATAAGATTGGTGTCCTTGTCTCAAAGCAGGATCATTGCCTGGTAGATTTATTACATCAGTGGCAGGAAGGCAGGCTTCCAGTAGATATAACTTGTGTAATAAGTAATCATCATAGAGGTCCAAACACCCATGTGCATCGTTTTCTAGAAAGACACGGTATACCTTATCATTATCTAAATACAACCAAGGATGACAAAGGAGAAGGGGAGATACTGGAGTTGGTTCAAAACACTGATTTTTTAGTTCTTGCCAGGTACATGCAGATATTATCCGGTGATTTTTTAAAGAGCTATGGAAAGGATATAATTAACATTCATCATGGCCTTTTGCCATCATTCAAAGGTGGAAAACCATCTAAACAGGCTTTTGATGCAGGGGTGAAGCTTATTGGTGCAACAACTCATTTTGTGACGCAAGAACTTGATGCCGGGCCTATTATTGAACAGATGGTACTGGAGACAATTTGA

Protein Analysis

285

Amino Acids

31.83

Weight (kDa)

8.27

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Formyl_trans_N PF00551 131 - 282 2.2e-30 Formyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 218
AccI GTMKAC 1 cut(s) 547
AciI CCGC 1 cut(s) 204
AclWI GGATC 2 cut(s) 275, 426
AcoI YGGCCR 2 cut(s) 171, 293
AcsI RAATTY 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 2 cut(s) 644, 840
AgsI TTSAA 4 cut(s) 67, 614, 727, 830
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 3 cut(s) 82, 426, 638
AjuI GAANNNNNNNTTGG 2 cut(s) 47, 79
AleI CACNNNNGTG 1 cut(s) 518
AluBI AGCT 5 cut(s) 40, 178, 328, 678, 772
AluI AGCT 5 cut(s) 40, 178, 328, 678, 772
Alw26I GTCTC 2 cut(s) 412, 839
AlwI GGATC 2 cut(s) 275, 426
AoxI GGCC 5 cut(s) 59, 171, 293, 709, 818
ApoI RAATTY 1 cut(s) 269
AspLEI GCGC 1 cut(s) 221
AspS9I GGNCC 3 cut(s) 60, 506, 818
AsuC2I CCSGG 2 cut(s) 365, 817
AsuHPI GGTGA 2 cut(s) 674, 778
AvaII GGWCC 1 cut(s) 506
BaeGI GKGCMC 1 cut(s) 237
BaeI ACNNNNGTAYC 2 cut(s) 590, 623
BalI TGGCCA 2 cut(s) 173, 295
BanI GGYRCC 1 cut(s) 218
BanII GRGCYC 1 cut(s) 93
BarI GAAGNNNNNNTAC 2 cut(s) 582, 614
BbsI GAAGAC 2 cut(s) 133, 220
BccI CCATC 3 cut(s) 727, 748, 829
BciT130I CCWGG 3 cut(s) 84, 428, 640
BcnI CCSGG 2 cut(s) 365, 817
BcoDI GTCTC 2 cut(s) 412, 839
BfaI CTAG 1 cut(s) 533
BfoI RGCGCY 1 cut(s) 222
BglII AGATCT 1 cut(s) 353
Bme1390I CCNGG 5 cut(s) 84, 365, 428, 640, 817
Bme18I GGWCC 1 cut(s) 506
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 3 cut(s) 60, 506, 818
BmiI GGNNCC 3 cut(s) 90, 220, 369
BmrFI CCNGG 5 cut(s) 84, 365, 428, 640, 817
BmsI GCATC 5 cut(s) 29, 145, 532, 748, 802
BpiI GAAGAC 2 cut(s) 133, 220
BpmI CTGGAG 1 cut(s) 623
BpuEI CTTGAG 1 cut(s) 94
BpuMI CCSGG 2 cut(s) 365, 817
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 82, 83, 573
BsaWI WCCGGW 1 cut(s) 659
Bse1I ACTGG 3 cut(s) 467, 606, 846
Bse3DI GCAATG 3 cut(s) 145, 189, 421
BseBI CCWGG 3 cut(s) 84, 428, 640
BseDI CCNNGG 3 cut(s) 82, 83, 573
BseGI GGATG 2 cut(s) 316, 583
BseMI GCAATG 3 cut(s) 145, 189, 421
BseNI ACTGG 3 cut(s) 467, 606, 846
BseSI GKGCMC 1 cut(s) 237
BshFI GGCC 5 cut(s) 61, 173, 295, 711, 820
BshNI GGYRCC 1 cut(s) 218
BsiSI CCGG 3 cut(s) 364, 660, 816
BsmAI GTCTC 2 cut(s) 412, 839
BsnI GGCC 5 cut(s) 61, 173, 295, 711, 820
Bsp1286I GDGCHC 2 cut(s) 93, 237
Bsp143I GATC 3 cut(s) 280, 353, 418
BspACI CCGC 1 cut(s) 204
BspANI GGCC 5 cut(s) 61, 173, 295, 711, 820
BspLI GGNNCC 3 cut(s) 90, 220, 369
BspPI GGATC 2 cut(s) 275, 426
BspT107I GGYRCC 1 cut(s) 218
BsrDI GCAATG 3 cut(s) 145, 189, 421
BsrI ACTGG 3 cut(s) 467, 606, 846
BssECI CCNNGG 3 cut(s) 82, 83, 573
BssMI GATC 3 cut(s) 280, 353, 418
BssNAI GTATAC 1 cut(s) 548
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 573
Bst1107I GTATAC 1 cut(s) 548
Bst2UI CCWGG 3 cut(s) 84, 428, 640
Bst4CI ACNGT 1 cut(s) 545
Bst6I CTCTTC 1 cut(s) 106
BstACI GRCGYC 1 cut(s) 219
BstC8I GCNNGC 2 cut(s) 223, 461
BstF5I GGATG 2 cut(s) 316, 583
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 3 cut(s) 283, 356, 421
BstMAI GTCTC 2 cut(s) 412, 839
BstMBI GATC 3 cut(s) 280, 353, 418
BstMWI GCNNNNNNNGC 1 cut(s) 758
BstNI CCWGG 3 cut(s) 84, 428, 640
BstNSI RCATGY 1 cut(s) 649
BstSCI CCNGG 5 cut(s) 82, 363, 426, 638, 815
BstSLI GKGCMC 1 cut(s) 237
BstV2I GAAGAC 2 cut(s) 133, 220
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BstZ17I GTATAC 1 cut(s) 548
BsuRI GGCC 5 cut(s) 61, 173, 295, 711, 820
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 2 cut(s) 316, 583
BtsIMutI CAGTG 2 cut(s) 452, 618
Cac8I GCNNGC 2 cut(s) 223, 461
CfoI GCGC 1 cut(s) 221
Cfr13I GGNCC 3 cut(s) 60, 506, 818
CseI GACGC 2 cut(s) 214, 808
Csp6I GTAC 2 cut(s) 643, 839
CviAII CATG 3 cut(s) 518, 646, 707
CviQI GTAC 2 cut(s) 643, 839
DinI GGCGCC 1 cut(s) 220
DpnI GATC 3 cut(s) 282, 355, 420
DpnII GATC 3 cut(s) 280, 353, 418
DraI TTTAAA 1 cut(s) 672
EaeI YGGCCR 2 cut(s) 171, 293
Eam1104I CTCTTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 106
EciI GGCGGA 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 573
Eco24I GRGCYC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 506
EcoO109I RGGNCCY 1 cut(s) 60
EcoRII CCWGG 3 cut(s) 82, 426, 638
EcoT14I CCWWGG 1 cut(s) 573
EcoT38I GRGCYC 1 cut(s) 93
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 3 cut(s) 521, 649, 710
FalI AAGNNNNNCTT 4 cut(s) 47, 79, 447, 479
FatI CATG 3 cut(s) 517, 645, 706
FblI GTMKAC 1 cut(s) 547
FokI GGATG 2 cut(s) 323, 590
FriOI GRGCYC 1 cut(s) 93
FspBI CTAG 1 cut(s) 533
GlaI GCGC 1 cut(s) 220
GsuI CTGGAG 1 cut(s) 623
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 5 cut(s) 61, 173, 295, 711, 820
HapII CCGG 3 cut(s) 364, 660, 816
HgaI GACGC 2 cut(s) 214, 808
HhaI GCGC 1 cut(s) 221
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 3 cut(s) 521, 649, 710
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 2 cut(s) 38, 770
HinfI GANTC 3 cut(s) 78, 98, 132
HpaI GTTAAC 1 cut(s) 336
HpaII CCGG 3 cut(s) 364, 660, 816
HphI GGTGA 2 cut(s) 674, 778
Hpy166II GTNNAC 2 cut(s) 336, 548
Hpy188I TCNGA 3 cut(s) 47, 184, 353
Hpy188III TCNNGA 3 cut(s) 261, 377, 533
Hpy8I GTNNAC 2 cut(s) 336, 548
HpyAV CCTTC 3 cut(s) 73, 449, 584
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4IV ACGT 2 cut(s) 138, 249
HpyCH4V TGCA 7 cut(s) 158, 189, 347, 523, 649, 761, 782
HpyF10VI GCNNNNNNNGC 1 cut(s) 758
HpySE526I ACGT 2 cut(s) 138, 249
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 3 cut(s) 521, 649, 710
HspAI GCGC 1 cut(s) 219
KasI GGCGCC 1 cut(s) 218
KspAI GTTAAC 1 cut(s) 336
Kzo9I GATC 3 cut(s) 280, 353, 418
LmnI GCTCC 1 cut(s) 88
LweI GCATC 5 cut(s) 29, 145, 532, 748, 802
MaeI CTAG 1 cut(s) 533
MaeII ACGT 2 cut(s) 138, 249
MaeIII GTNAC 1 cut(s) 796
MalI GATC 3 cut(s) 282, 355, 420
MboI GATC 3 cut(s) 280, 353, 418
MboII GAAGA 4 cut(s) 93, 99, 133, 220
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 2 cut(s) 93, 237
MlsI TGGCCA 2 cut(s) 173, 295
MluCI AATT 3 cut(s) 269, 693, 849
MluNI TGGCCA 2 cut(s) 173, 295
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 1 cut(s) 92
MnlI CCTC 8 cut(s) 17, 41, 51, 104, 129, 294, 307, 497
Mox20I TGGCCA 2 cut(s) 173, 295
MscI TGGCCA 2 cut(s) 173, 295
MseI TTAA 4 cut(s) 288, 335, 671, 696
MslI CAYNNNNRTG 1 cut(s) 518
Msp20I TGGCCA 2 cut(s) 173, 295
MspI CCGG 3 cut(s) 364, 660, 816
MspR9I CCNGG 5 cut(s) 84, 365, 428, 640, 817
MvaI CCWGG 3 cut(s) 84, 428, 640
MwoI GCNNNNNNNGC 1 cut(s) 758
NarI GGCGCC 1 cut(s) 219
NciI CCSGG 2 cut(s) 365, 817
NdeII GATC 3 cut(s) 280, 353, 418
NlaIII CATG 3 cut(s) 521, 649, 710
NlaIV GGNNCC 3 cut(s) 90, 220, 369
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 1 cut(s) 796
NspI RCATGY 1 cut(s) 649
OliI CACNNNNGTG 1 cut(s) 518
PasI CCCWGGG 1 cut(s) 83
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 222
PfeI GAWTC 2 cut(s) 78, 132
PleI GAGTC 1 cut(s) 92
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 92
Psp6I CCWGG 3 cut(s) 82, 426, 638
PspGI CCWGG 3 cut(s) 82, 426, 638
PspN4I GGNNCC 3 cut(s) 90, 220, 369
PspPI GGNCC 3 cut(s) 60, 506, 818
PsrI GAACNNNNNNTAC 2 cut(s) 822, 854
PsuI RGATCY 1 cut(s) 353
RsaI GTAC 2 cut(s) 644, 840
RsaNI GTAC 2 cut(s) 643, 839
RseI CAYNNNNRTG 1 cut(s) 518
SaqAI TTAA 4 cut(s) 288, 335, 671, 696
Sau3AI GATC 3 cut(s) 280, 353, 418
Sau96I GGNCC 3 cut(s) 60, 506, 818
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 5 cut(s) 84, 365, 428, 640, 817
SduI GDGCHC 2 cut(s) 93, 237
SfaNI GCATC 5 cut(s) 29, 145, 532, 748, 802
SfoI GGCGCC 1 cut(s) 220
SinI GGWCC 1 cut(s) 506
SmiMI CAYNNNNRTG 1 cut(s) 518
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 3 cut(s) 269, 693, 849
SsiI CCGC 1 cut(s) 204
SspDI GGCGCC 1 cut(s) 218
SspMI CTAG 1 cut(s) 533
StyD4I CCNGG 5 cut(s) 82, 363, 426, 638, 815
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 1 cut(s) 545
TaiI ACGT 2 cut(s) 141, 252
TaqI TCGA 3 cut(s) 96, 279, 378
TasI AATT 3 cut(s) 269, 693, 849
TfiI GAWTC 2 cut(s) 78, 132
Tru1I TTAA 4 cut(s) 288, 335, 671, 696
Tru9I TTAA 4 cut(s) 288, 335, 671, 696
TscAI CASTG 2 cut(s) 452, 625
TseFI GTSAC 1 cut(s) 796
Tsp45I GTSAC 1 cut(s) 796
TspDTI ATGAA 3 cut(s) 17, 93, 692
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 2 cut(s) 452, 625
VpaK11BI GGWCC 1 cut(s) 506
XapI RAATTY 1 cut(s) 269
XbaI TCTAGA 1 cut(s) 532
XceI RCATGY 1 cut(s) 649
XmiI GTMKAC 1 cut(s) 547
XspI CTAG 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.