Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004003

Physical Location & Seq

Genomic Coordinates Reverse (-)

20409717 .. 20420600

Gene Structure 10884 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1083300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3648 bp

ATGAACACTCAGTTTGAGTCACACAAAGATGAGAATGGGGGGTCAAAGTCACACATAGATGAAAAGGAGGGGTTTGAAAATTTAAATGGGACTTCAACTGCAAATGCTGAACCAGTGGAAAGTTTCGGCAGCACAATTGAAGACAACCAGATATTATCGCTGGTTGAGTGGTTGAATTTTATTGTTCCTTATTTAAGATTGCCATTGGATGCTTCTGAGGAGGAACTCAGAGCCTGCTTGGTTGATGGGACTGTTTTATGCAGAATTTTAAATAAGTTGTGTCCTGGTTCAGTTGAAATGGGAGGGAGTTCGGACCCCGGTTTCGCAAATGTCAAGAGGTTTCTGTCAGCTGTGGATGAATTAGGATTCCCTCGCTTTGACCTAATAGACCTAGAGCAGGGATCTCTTGTGCCAGTTTTGCAATGCCTTGGTGCACTTAAATCTTCTTTTGATTTTGGTTTTTGGGGAGAGAACAGTAAACACCTAACAAGGACAGAAAGTGATTTGGTGGAGGTAGAATCTTTAGAGGGACTTTATCGTTCCCGAGGGGATATCTCAACATTTGGACTACAATCTACTCAAAATAGACAAGATACTCAAGGAAATTCAAGTGACTCAACATCTCATATGACAGATCCATCAGCTGATTTAGTACATCATATTGGACAGCAACTGAAGCAGGGGAGTTTTGTTGATTTCTCTGATGCTAAGATTTTGGAATCAGTCAAATCAAGCAGTTTAGATAATGCATCTACTCGATCACTTTTCAATGTTGGGAATAAGATTCTGGATGACAGTATCGACAGAAACAATGGTGATGTACCTCATTGTGTAGCATATCTTCTAAGAAAAGTCATGCAAGTGATTGAACAGAGATTCGCAAATCAGGCGGTGAACTTGAGAATTCAAAACAACATATATAAGGCCCGTGAGGAGAAGTTTCTATTGAAAATAAAAGTACTTGAAACCCTTGCATCCGGGACTACCGAAGAAAATGAGGTTGTTCTGAAACAGCTTCAGAATATCAAGCTTGAGAAGTTTGATATAGAGGAGCAGAAAAAACTTGAGGAGGAGGATGCAGTGCGATTAAAGGAAGAGAAGTATCACAGGGATTCAGAGATTTCAACACTGAAACAAGAACTAGAATTGGCCACAAGTCGACATGAAAGTTACTGCCAGAGGTTAGAGGAAAATGCAAAAGAAGCTAAACTTCAACTGGAAAGGAAGTTAAAAGAACTTGAGAGCATACTAACAGATTCAAAGCAGAACCAGAAAGAACTAGAGGCATCTTTGGAAAATGAATCAGGGAGATGGAAAAATAAAGAACGCACTTATCAAAGTTTCGTAACTTACCAATCTGGAGCTTTGCAGGAATTAAGTGGCGCTTTGGAGTCCACTAGACATGAAATCTTGAAGACAAAAAGAAGTTGCTCCGAAGATTTTAAGTACTTGGCTTTGAAGCTTAAAGGACTGACAGATGCTGCTGAAAAGTATCATGCTGTTCTTGATGAAAATCGGAAATTGTATAATGAGGTTCAGGATTTGAAAGGTAATATTAGAGTGTACTGTCGAATAAGACCATTCCTTCCGGGTCAAAGCCAAAAGCAAACCACCATAGAATATGTTGGTGAAAATGGTGATCTAGTTATTGCAAATCCCTCTAAACAAGGAAAGGACAGTCGCAGGCTGTTTAAATTTAACAAAGTATTTGGTCCTGCAGCTACACAAGAGGACGTATTTTTAGACACCCAACCATTGATTCGTTCTGTCCTTGATGGATACAATGTTTGTATATTTGCCTATGGTCAAACTGGTTCAGGAAAGACATATACCATGACTGGGCCGAGTGTATCATCAACAGATGACTGGGGTGTCAACTATCGAGCATTAAATGATCTTTTCCAAATATCTCAGAGCAGGGAACACTCCATAGCTTATGAAGTTGGTGTTCAAATGGTAGAGATATATAATGAACAAGTTCGTGATCTACTCTCAAATTTGGTTTCCTTTAACTTGCACACACTTGGGATTTGGAATACTACCCAACCAAATGGGTTGGCTGTCCCTGATGCAAGCATGCATCCTGTTAATTCAACTGCAGATGTCCTAAAGTTAATGAATATTGGGTTGATGAACCGGGCAGTTGGTGCCACGGCCCTAAATGAAAGAAGCAGCAGATCTCATAGGTTATATTCTGATGTTTTGACTGTTCATGTTCGTGGCGTGGACTTGAAGTCTGACACTTCAATGCGTGGAAGTCTACACCTCGTGGATCTTGCTGGAAGCGAACGAGTGGATCGCTCTGAAGCTACTGGAGATAGGCTTAGGGAGGCACAGCATATAAACAAATCACTGTCTGCTCTTGGGGATGTGATTTTTGCTCTTGCACAGAAGTCTTCACATGTACCGTATAGAAACAGCAAATTAACTCAAGTGCTTCAGAGTTCTTTAGGTGGTCAAGCAAAGACACTTATGTTTGTACAGCTGAATCCGGATGTTCAATCCTTTGCTGAAACTACAAGTACACTGAAGTTTGCAGAGAGGGTTTCTGGTGTTGAATTAGGTGCTGCCCGGAGCAACAAAGAGGGAAGATATGTGAGAGAACTTATGGAACAGGTGGCATCCTTCAGGGACACAATTGCTAAGAAAGATGAGGAGATTGAACGATTGCTGAAGGTTAATAGTAATGGGTTACACCGTGGCATGAGCTCACTAAGATTTGCGTCTTCCTCTCCAAGAAGACATTCAATTGGGTCTCCTCGACTGAGCCAAAAAAGTCCAGGTAGAAGACGCTCAGGATATTATAGTGATAAGCGTTCTGAAGTTAGTTCTCAGCTGTCAATAGATGACCTTAGAAATCGTAGGGAAGATTTTCCTCAGTCAAAGAATGGAGGTAGTTCTCAGTCAATGGATGACTTTAGACATCACAAGGAACATTTGCCAGAGACAAAGCATGAAGGTGAGGCAAACCATTCTGAAGATAGTTCTCAGCAGTCAGCAGATGACTTAAAAGATACTAAGGAACATTCGCTTCGGTCATATCATGGAGGTGAGACAAAGAGTCCTGAAGCTGGTTCTGATAAGTCACTGGATGACTTTAGAAATCATAATGGCCATTCAACTCATCCAAACTGTGGAGGTAGGGGAAAGCATTCTGACGGCAGATCTGAGCAGTCAATAGATGACTTTAGACGTCACAAGGAGCACTCGACTGAACAAAAGCATGGAGATGAGTCAAGTCAAAAAATCATTGAAGATTTTGAGCTCTTGGGCTTTGGTGAGGAAGATTCTGGGGAGAGATTAAGCGACATATCTGATGGCGGTCTCTCAATGGGAACAGAAACTGAGGGTTCCATGGCCAGTTTTATGGAGTTTTCTCTTTTCCCTGATGTTACAAAACCAACAGAAAATACAAATGCTGGAAATACAGATGTCACGAAAACTCAAGCTGCTCGAGGCGAGAAGACACCGTCCCAGCGCACAGTAAGGTTTGCACCGTCTAAACTTCCAAAGCCGTCACCTAAGTTAGCAGAAACAAAAGCTACTCGGACATCAGTTATCAGGAGTTCCTCAAAGGCTTTGCCAAGTCCTCGGAAATCTGCTGCCGGCAGCAGCTCATCAGTGAGGTCTTCCAAACGCTGGAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0005886 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin Microtub_bd

Protein Analysis

1216

Amino Acids

135.64

Weight (kDa)

6.06

Isoelectric Point (pI)

52.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	54 - 146	9.2e-07	Calponin homology (CH) domain
Microtub_bd	PF16796	505 - 664	2.5e-44	Microtubule binding
Kinesin	PF00225	524 - 850	1e-103	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004003)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63640 AT1G63640 AT1G63640 AT1G63640 AT1G63640 AT5G41310
fragaria_vesca	FvH4_5g02480 FvH4_5g02480 FvH4_5g02480
malus_domestica	MD06G1083300.v1.1 MD14G1105100.v1.1
prunus_persica	Prupe.5G100400_v2.0.a1 Prupe.5G100400_v2.0.a1
pyrus_communis	pycom06g08010 pycom14g10070
rosa_chinensis	RchiOBHm_Chr5g0040651 RchiOBHm_Chr7g0197631 RchiOBHm_Chr7g0197651
rosa_laevigata	RLG00000003921
rosa_multiflora	Rmu_sc0003916.1_g000019 Rmu_sc0006732.1_g000004
rosa_roxburghii	Rroxscaffold_3G00258310
rosa_rugosa	Rorug07G0042000 Rorug07G0042100
rosa_samantha	Rh5BG277300 Rh7AG166400 Rh7BG168500 Rh7BG168600 Rh7CG174900 Rh7DG168100 Rh7DG168200
rosa_wichuraiana	Rw5G025550 Rw7G014490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1871
AatII	GACGTC	1 cut(s)	3196
AccB1I	GGYRCC	1 cut(s)	2147
AccB7I	CCANNNNNTGG	2 cut(s)	3134, 3639
AccI	GTMKAC	2 cut(s)	1159, 2260
AccIII	TCCGGA	1 cut(s)	2491
AciI	CCGC	2 cut(s)	890, 3321
AclWI	GGATC	4 cut(s)	409, 629, 2280, 2304
AcoI	YGGCCR	3 cut(s)	1149, 3112, 3357
AcsI	RAATTY	7 cut(s)	79, 175, 264, 604, 903, 1694, 1996
AcyI	GRCGYC	1 cut(s)	3193
AdeI	CACNNNGTG	1 cut(s)	2269
AfaI	GTAC	8 cut(s)	654, 822, 960, 1448, 1565, 2406, 2481, 2524
AfiI	CCNNNNNNNGG	5 cut(s)	397, 1839, 3071, 3134, 3639
AflIII	ACRYGT	1 cut(s)	2401
AhdI	GACNNNNNGTC	2 cut(s)	2233, 3060
AjnI	CCWGG	2 cut(s)	283, 2779
AjuI	GAANNNNNNNTTGG	4 cut(s)	1743, 1775, 2728, 2760
Alw21I	GWGCWC	4 cut(s)	436, 2711, 3207, 3267
Alw26I	GTCTC	4 cut(s)	2760, 2939, 3047, 3329
Alw44I	GTGCAC	1 cut(s)	432
AlwI	GGATC	4 cut(s)	409, 629, 2280, 2304
AlwNI	CAGNNNCTG	4 cut(s)	234, 673, 1481, 3344
Ama87I	CYCGRG	2 cut(s)	543, 3453
Aor13HI	TCCGGA	1 cut(s)	2491
AoxI	GGCC	6 cut(s)	924, 1149, 1841, 2154, 3112, 3357
ApaLI	GTGCAC	1 cut(s)	432
ApeKI	GCWGC	9 cut(s)	129, 1481, 1718, 2172, 2567, 3449, 3602, 3609, 3612
ApoI	RAATTY	7 cut(s)	79, 175, 264, 604, 903, 1694, 1996
ArsI	GACNNNNNNTTYG	2 cut(s)	305, 337
Asp700I	GAANNNNTTC	4 cut(s)	1977, 2743, 2871, 3151
AspLEI	GCGC	2 cut(s)	1385, 3480
AspS9I	GGNCC	5 cut(s)	313, 925, 1712, 1841, 2155
AsuC2I	CCSGG	5 cut(s)	318, 979, 1590, 2138, 2572
AsuHPI	GGTGA	8 cut(s)	827, 904, 1640, 1649, 2972, 3062, 3290, 3510
AvaI	CYCGRG	2 cut(s)	543, 3453
AvaII	GGWCC	2 cut(s)	313, 1712
BaeGI	GKGCMC	1 cut(s)	436
BalI	TGGCCA	3 cut(s)	1151, 3114, 3359
BanI	GGYRCC	1 cut(s)	2147
BanII	GRGCYC	2 cut(s)	2711, 3267
BarI	GAAGNNNNNNTAC	2 cut(s)	825, 857
BauI	CACGAG	1 cut(s)	2267
BbsI	GAAGAC	8 cut(s)	147, 1421, 2388, 2718, 2746, 2794, 3470, 3621
Bbv12I	GWGCWC	4 cut(s)	436, 2711, 3207, 3267
BbvI	GCAGC	9 cut(s)	141, 1468, 1730, 2184, 2554, 3436, 3589, 3621, 3624
BccI	CCATC	5 cut(s)	239, 646, 1305, 1769, 3311
BceAI	ACGGC	3 cut(s)	2169, 3175, 3499
BciT130I	CCWGG	2 cut(s)	285, 2781
BciVI	GTATCC	1 cut(s)	1772
BcnI	CCSGG	5 cut(s)	318, 979, 1590, 2138, 2572
BcoDI	GTCTC	4 cut(s)	2760, 2939, 3047, 3329
BfaI	CTAG	5 cut(s)	392, 1142, 1280, 1398, 1643
BfmI	CTRYAG	2 cut(s)	1716, 2097
BfoI	RGCGCY	1 cut(s)	1386
BfuI	GTATCC	1 cut(s)	1772
BglII	AGATCT	2 cut(s)	2177, 3164
BisI	GCNGC	9 cut(s)	130, 1482, 1719, 2173, 2568, 3450, 3603, 3610, 3613
BlsI	GCNGC	9 cut(s)	131, 1483, 1720, 2174, 2569, 3451, 3604, 3611, 3614
BmcAI	AGTACT	2 cut(s)	960, 1448
Bme1390I	CCNGG	7 cut(s)	285, 318, 979, 1590, 2138, 2572, 2781
Bme18I	GGWCC	2 cut(s)	313, 1712
BmeRI	GACNNNNNGTC	2 cut(s)	2233, 3060
BmeT110I	CYCGRG	2 cut(s)	543, 3453
BmgT120I	GGNCC	5 cut(s)	313, 925, 1712, 1841, 2155
BmiI	GGNNCC	3 cut(s)	315, 2149, 3352
BmrFI	CCNGG	7 cut(s)	285, 318, 979, 1590, 2138, 2572, 2781
BmrI	ACTGGG	2 cut(s)	1848, 1876
BmuI	ACTGGG	2 cut(s)	1848, 1876
BpiI	GAAGAC	8 cut(s)	147, 1421, 2388, 2718, 2746, 2794, 3470, 3621
BpmI	CTGGAG	2 cut(s)	1380, 2334
Bpu10I	CCTNAGC	2 cut(s)	2324, 2794
BpuEI	CTTGAG	7 cut(s)	582, 919, 1052, 1085, 1259, 2415, 3429
BpuMI	CCSGG	5 cut(s)	318, 979, 1590, 2138, 2572
BsaBI	GATNNNNATC	1 cut(s)	1512
BsaHI	GRCGYC	1 cut(s)	3193
BsaI	GGTCTC	2 cut(s)	2760, 3329
BsaJI	CCNNGG	7 cut(s)	316, 427, 544, 2151, 2698, 3354, 3590
BsaWI	WCCGGW	1 cut(s)	2491
Bsc4I	CCNNNNNNNGG	5 cut(s)	397, 1839, 3071, 3134, 3639
Bse118I	RCCGGY	1 cut(s)	3605
Bse1I	ACTGG	9 cut(s)	113, 413, 1221, 1816, 1843, 1871, 2317, 3093, 3360
Bse3DI	GCAATG	1 cut(s)	428
Bse8I	GATNNNNATC	1 cut(s)	1512
BseAI	TCCGGA	1 cut(s)	2491
BseBI	CCWGG	2 cut(s)	285, 2781
BseDI	CCNNGG	7 cut(s)	316, 427, 544, 2151, 2698, 3354, 3590
BseJI	GATNNNNATC	1 cut(s)	1512
BseLI	CCNNNNNNNGG	5 cut(s)	397, 1839, 3071, 3134, 3639
BseMI	GCAATG	1 cut(s)	428
BseNI	ACTGG	9 cut(s)	113, 413, 1221, 1816, 1843, 1871, 2317, 3093, 3360
BseRI	GAGGAG	7 cut(s)	233, 947, 1064, 1082, 1085, 2669, 2748
BseSI	GKGCMC	1 cut(s)	436
BseXI	GCAGC	9 cut(s)	141, 1468, 1730, 2184, 2554, 3436, 3589, 3621, 3624
BseYI	CCCAGC	1 cut(s)	3474
BshFI	GGCC	6 cut(s)	926, 1151, 1843, 2156, 3114, 3359
BshNI	GGYRCC	1 cut(s)	2147
BsiHKAI	GWGCWC	4 cut(s)	436, 2711, 3207, 3267
BsiHKCI	CYCGRG	2 cut(s)	543, 3453
BsiSI	CCGG	7 cut(s)	318, 978, 1589, 2137, 2492, 2572, 3606
BslFI	GGGAC	7 cut(s)	103, 262, 543, 994, 2048, 2645, 3457
BslI	CCNNNNNNNGG	5 cut(s)	397, 1839, 3071, 3134, 3639
BsmAI	GTCTC	4 cut(s)	2760, 2939, 3047, 3329
BsmFI	GGGAC	7 cut(s)	103, 262, 543, 994, 2048, 2645, 3457
BsmI	GAATGC	1 cut(s)	3151
BsnI	GGCC	6 cut(s)	926, 1151, 1843, 2156, 3114, 3359
Bso31I	GGTCTC	2 cut(s)	2760, 3329
BsoBI	CYCGRG	2 cut(s)	543, 3453
Bsp1286I	GDGCHC	4 cut(s)	436, 2711, 3207, 3267
Bsp13I	TCCGGA	1 cut(s)	2491
Bsp1407I	TGTACA	1 cut(s)	2479
Bsp19I	CCATGG	1 cut(s)	3354
BspACI	CCGC	2 cut(s)	890, 3321
BspANI	GGCC	6 cut(s)	926, 1151, 1843, 2156, 3114, 3359
BspEI	TCCGGA	1 cut(s)	2491
BspLI	GGNNCC	3 cut(s)	315, 2149, 3352
BspMAI	CTGCAG	2 cut(s)	1720, 2101
BspPI	GGATC	4 cut(s)	409, 629, 2280, 2304
BspT107I	GGYRCC	1 cut(s)	2147
BspTNI	GGTCTC	2 cut(s)	2760, 3329
BsrDI	GCAATG	1 cut(s)	428
BsrFI	RCCGGY	1 cut(s)	3605
BsrGI	TGTACA	1 cut(s)	2479
BsrI	ACTGG	9 cut(s)	113, 413, 1221, 1816, 1843, 1871, 2317, 3093, 3360
BssAI	RCCGGY	1 cut(s)	3605
BssECI	CCNNGG	7 cut(s)	316, 427, 544, 2151, 2698, 3354, 3590
BssNI	GRCGYC	1 cut(s)	3193
BssSI	CACGAG	1 cut(s)	2267
BssT1I	CCWWGG	2 cut(s)	427, 3354
Bst2BI	CACGAG	1 cut(s)	2267
Bst2UI	CCWGG	2 cut(s)	285, 2781
Bst6I	CTCTTC	1 cut(s)	1089
BstACI	GRCGYC	1 cut(s)	3193
BstAPI	GCANNNNNTGC	1 cut(s)	2147
BstAUI	TGTACA	1 cut(s)	2479
BstC8I	GCNNGC	5 cut(s)	235, 1685, 2074, 2078, 3607
BstDSI	CCRYGG	3 cut(s)	2151, 2698, 3354
BstENI	CCTNNNNNAGG	1 cut(s)	395
BstH2I	RGCGCY	1 cut(s)	1386
BstHHI	GCGC	2 cut(s)	1385, 3480
BstMAI	GTCTC	4 cut(s)	2760, 2939, 3047, 3329
BstMWI	GCNNNNNNNGC	5 cut(s)	418, 676, 887, 1202, 2147
BstNI	CCWGG	2 cut(s)	285, 2781
BstNSI	RCATGY	2 cut(s)	2080, 2405
BstSCI	CCNGG	7 cut(s)	283, 316, 977, 1588, 2136, 2570, 2779
BstSFI	CTRYAG	2 cut(s)	1716, 2097
BstSLI	GKGCMC	1 cut(s)	436
BstV1I	GCAGC	9 cut(s)	141, 1468, 1730, 2184, 2554, 3436, 3589, 3621, 3624
BstV2I	GAAGAC	8 cut(s)	147, 1421, 2388, 2718, 2746, 2794, 3470, 3621
BstX2I	RGATCY	5 cut(s)	401, 634, 2177, 2272, 3164
BstXI	CCANNNNNNTGG	2 cut(s)	2051, 3367
BstYI	RGATCY	5 cut(s)	401, 634, 2177, 2272, 3164
BsuI	GTATCC	1 cut(s)	1772
BsuRI	GGCC	6 cut(s)	926, 1151, 1843, 2156, 3114, 3359
BtgI	CCRYGG	3 cut(s)	2151, 2698, 3354
BtsI	GCAGTG	1 cut(s)	1086
BtsIMutI	CAGTG	7 cut(s)	120, 1086, 1127, 2351, 2525, 3086, 3627
Cac8I	GCNNGC	5 cut(s)	235, 1685, 2074, 2078, 3607
CaiI	CAGNNNCTG	4 cut(s)	234, 673, 1481, 3344
CfoI	GCGC	2 cut(s)	1385, 3480
Cfr10I	RCCGGY	1 cut(s)	3605
Cfr13I	GGNCC	5 cut(s)	313, 925, 1712, 1841, 2155
CseI	GACGC	2 cut(s)	2712, 2799
Csp6I	GTAC	8 cut(s)	653, 821, 959, 1447, 1564, 2405, 2480, 2523
CviQI	GTAC	8 cut(s)	653, 821, 959, 1447, 1564, 2405, 2480, 2523
DraI	TTTAAA	3 cut(s)	84, 270, 1693
DraIII	CACNNNGTG	1 cut(s)	2269
DrdI	GACNNNNNNGTC	1 cut(s)	1871
DriI	GACNNNNNGTC	2 cut(s)	2233, 3060
DseDI	GACNNNNNNGTC	1 cut(s)	1871
EaeI	YGGCCR	3 cut(s)	1149, 3112, 3357
Eam1104I	CTCTTC	1 cut(s)	1089
Eam1105I	GACNNNNNGTC	2 cut(s)	2233, 3060
EarI	CTCTTC	1 cut(s)	1089
Ecl136II	GAGCTC	2 cut(s)	2709, 3265
Eco130I	CCWWGG	2 cut(s)	427, 3354
Eco24I	GRGCYC	2 cut(s)	2711, 3267
Eco31I	GGTCTC	2 cut(s)	2760, 3329
Eco32I	GATATC	1 cut(s)	553
Eco47I	GGWCC	2 cut(s)	313, 1712
Eco53kI	GAGCTC	2 cut(s)	2709, 3265
Eco88I	CYCGRG	2 cut(s)	543, 3453
EcoICRI	GAGCTC	2 cut(s)	2709, 3265
EcoNI	CCTNNNNNAGG	1 cut(s)	395
EcoRI	GAATTC	1 cut(s)	903
EcoRII	CCWGG	2 cut(s)	283, 2779
EcoRV	GATATC	1 cut(s)	553
EcoT14I	CCWWGG	2 cut(s)	427, 3354
EcoT22I	ATGCAT	2 cut(s)	751, 2082
EcoT38I	GRGCYC	2 cut(s)	2711, 3267
ErhI	CCWWGG	2 cut(s)	427, 3354
FalI	AAGNNNNNCTT	4 cut(s)	1194, 1226, 1369, 1401
FaqI	GGGAC	7 cut(s)	103, 262, 543, 994, 2048, 2645, 3457
FauNDI	CATATG	1 cut(s)	627
FblI	GTMKAC	2 cut(s)	1159, 2260
Fnu4HI	GCNGC	9 cut(s)	130, 1482, 1719, 2173, 2568, 3450, 3603, 3610, 3613
FriOI	GRGCYC	2 cut(s)	2711, 3267
Fsp4HI	GCNGC	9 cut(s)	130, 1482, 1719, 2173, 2568, 3450, 3603, 3610, 3613
FspBI	CTAG	5 cut(s)	392, 1142, 1280, 1398, 1643
GlaI	GCGC	2 cut(s)	1384, 3479
GluI	GCNGC	9 cut(s)	130, 1482, 1719, 2173, 2568, 3450, 3603, 3610, 3613
GsaI	CCCAGC	1 cut(s)	3478
GsuI	CTGGAG	2 cut(s)	1380, 2334
HaeII	RGCGCY	1 cut(s)	1386
HaeIII	GGCC	6 cut(s)	926, 1151, 1843, 2156, 3114, 3359
HapII	CCGG	7 cut(s)	318, 978, 1589, 2137, 2492, 2572, 3606
HgaI	GACGC	2 cut(s)	2712, 2799
HhaI	GCGC	2 cut(s)	1385, 3480
Hin1I	GRCGYC	1 cut(s)	3193
Hin6I	GCGC	2 cut(s)	1383, 3478
HinP1I	GCGC	2 cut(s)	1383, 3478
HincII	GTYRAC	2 cut(s)	1160, 1876
HindII	GTYRAC	2 cut(s)	1160, 1876
HindIII	AAGCTT	2 cut(s)	1028, 1460
HpaII	CCGG	7 cut(s)	318, 978, 1589, 2137, 2492, 2572, 3606
HphI	GGTGA	8 cut(s)	827, 904, 1640, 1649, 2972, 3062, 3290, 3510
HpyAV	CCTTC	4 cut(s)	1595, 2635, 2668, 2951
HpyCH4IV	ACGT	2 cut(s)	1734, 3193
HpyF10VI	GCNNNNNNNGC	5 cut(s)	418, 676, 887, 1202, 2147
HpySE526I	ACGT	2 cut(s)	1734, 3193
Hsp92I	GRCGYC	1 cut(s)	3193
HspAI	GCGC	2 cut(s)	1383, 3478
Kpn2I	TCCGGA	1 cut(s)	2491
KroI	GCCGGC	1 cut(s)	3605
KroNI	GCCGGC	1 cut(s)	3607
LmnI	GCTCC	5 cut(s)	1051, 1361, 1436, 2574, 3202
Lsp1109I	GCAGC	9 cut(s)	141, 1468, 1730, 2184, 2554, 3436, 3589, 3621, 3624
MaeI	CTAG	5 cut(s)	392, 1142, 1280, 1398, 1643
MaeII	ACGT	2 cut(s)	1734, 3193
MfeI	CAATTG	3 cut(s)	135, 2637, 2748
MflI	RGATCY	5 cut(s)	401, 634, 2177, 2272, 3164
MhlI	GDGCHC	4 cut(s)	436, 2711, 3207, 3267
MlsI	TGGCCA	3 cut(s)	1151, 3114, 3359
MluNI	TGGCCA	3 cut(s)	1151, 3114, 3359
MlyI	GAGTC	5 cut(s)	26, 608, 1400, 3070, 3242
Mox20I	TGGCCA	3 cut(s)	1151, 3114, 3359
Mph1103I	ATGCAT	2 cut(s)	751, 2082
MroI	TCCGGA	1 cut(s)	2491
MroNI	GCCGGC	1 cut(s)	3605
MroXI	GAANNNNTTC	4 cut(s)	1977, 2743, 2871, 3151
MscI	TGGCCA	3 cut(s)	1151, 3114, 3359
MslI	CAYNNNNRTG	8 cut(s)	27, 57, 860, 2217, 2246, 2958, 3048, 3228
Msp20I	TGGCCA	3 cut(s)	1151, 3114, 3359
MspA1I	CMGCKG	4 cut(s)	350, 644, 2485, 2836
MspI	CCGG	7 cut(s)	318, 978, 1589, 2137, 2492, 2572, 3606
MspR9I	CCNGG	7 cut(s)	285, 318, 979, 1590, 2138, 2572, 2781
MunI	CAATTG	3 cut(s)	135, 2637, 2748
Mva1269I	GAATGC	1 cut(s)	3151
MvaI	CCWGG	2 cut(s)	285, 2781
MwoI	GCNNNNNNNGC	5 cut(s)	418, 676, 887, 1202, 2147
NaeI	GCCGGC	1 cut(s)	3607
NciI	CCSGG	5 cut(s)	318, 979, 1590, 2138, 2572
NcoI	CCATGG	1 cut(s)	3354
NdeI	CATATG	1 cut(s)	627
NgoMIV	GCCGGC	1 cut(s)	3605
NlaIV	GGNNCC	3 cut(s)	315, 2149, 3352
NmeAIII	GCCGAG	1 cut(s)	1869
NmuCI	GTSAC	7 cut(s)	18, 48, 611, 3084, 3194, 3433, 3516
NsiI	ATGCAT	2 cut(s)	751, 2082
NspI	RCATGY	2 cut(s)	2080, 2405
PaeI	GCATGC	1 cut(s)	2080
PaeR7I	CTCGAG	1 cut(s)	3453
PciI	ACATGT	1 cut(s)	2401
PctI	GAATGC	1 cut(s)	3151
PdiI	GCCGGC	1 cut(s)	3607
PdmI	GAANNNNTTC	4 cut(s)	1977, 2743, 2871, 3151
PflFI	GACNNNGTC	1 cut(s)	3469
PflMI	CCANNNNNTGG	2 cut(s)	3134, 3639
PfoI	TCCNGGA	1 cut(s)	977
PkrI	GCNGC	9 cut(s)	131, 1483, 1720, 2174, 2569, 3451, 3604, 3611, 3614
PleI	GAGTC	5 cut(s)	25, 608, 1399, 3069, 3241
PpsI	GAGTC	5 cut(s)	25, 608, 1399, 3069, 3241
PscI	ACATGT	1 cut(s)	2401
Psp124BI	GAGCTC	2 cut(s)	2711, 3267
Psp6I	CCWGG	2 cut(s)	283, 2779
PspFI	CCCAGC	1 cut(s)	3474
PspGI	CCWGG	2 cut(s)	283, 2779
PspN4I	GGNNCC	3 cut(s)	315, 2149, 3352
PspPI	GGNCC	5 cut(s)	313, 925, 1712, 1841, 2155
PspXI	VCTCGAGB	1 cut(s)	3453
PstI	CTGCAG	2 cut(s)	1720, 2101
PstNI	CAGNNNCTG	4 cut(s)	234, 673, 1481, 3344
PsuI	RGATCY	5 cut(s)	401, 634, 2177, 2272, 3164
PsyI	GACNNNGTC	1 cut(s)	3469
PvuII	CAGCTG	4 cut(s)	350, 644, 2485, 2836
RsaI	GTAC	8 cut(s)	654, 822, 960, 1448, 1565, 2406, 2481, 2524
RsaNI	GTAC	8 cut(s)	653, 821, 959, 1447, 1564, 2405, 2480, 2523
RseI	CAYNNNNRTG	8 cut(s)	27, 57, 860, 2217, 2246, 2958, 3048, 3228
SacI	GAGCTC	2 cut(s)	2711, 3267
SalI	GTCGAC	1 cut(s)	1158
SatI	GCNGC	9 cut(s)	130, 1482, 1719, 2173, 2568, 3450, 3603, 3610, 3613
Sau96I	GGNCC	5 cut(s)	313, 925, 1712, 1841, 2155
ScaI	AGTACT	2 cut(s)	960, 1448
SchI	GAGTC	5 cut(s)	26, 608, 1400, 3070, 3242
ScrFI	CCNGG	7 cut(s)	285, 318, 979, 1590, 2138, 2572, 2781
SduI	GDGCHC	4 cut(s)	436, 2711, 3207, 3267
SfcI	CTRYAG	2 cut(s)	1716, 2097
Sfr274I	CTCGAG	1 cut(s)	3453
SinI	GGWCC	2 cut(s)	313, 1712
SlaI	CTCGAG	1 cut(s)	3453
SmiI	ATTTAAAT	1 cut(s)	84
SmiMI	CAYNNNNRTG	8 cut(s)	27, 57, 860, 2217, 2246, 2958, 3048, 3228
SmlI	CTYRAG	8 cut(s)	597, 898, 1031, 1064, 1238, 2430, 3444, 3453
SmoI	CTYRAG	8 cut(s)	597, 898, 1031, 1064, 1238, 2430, 3444, 3453
SphI	GCATGC	1 cut(s)	2080
SsiI	CCGC	2 cut(s)	890, 3321
SspI	AATATT	2 cut(s)	1555, 2122
SspMI	CTAG	5 cut(s)	392, 1142, 1280, 1398, 1643
SstI	GAGCTC	2 cut(s)	2711, 3267
StyD4I	CCNGG	7 cut(s)	283, 316, 977, 1588, 2136, 2570, 2779
StyI	CCWWGG	2 cut(s)	427, 3354
SwaI	ATTTAAAT	1 cut(s)	84
TaiI	ACGT	2 cut(s)	1737, 3196
TaqI	TCGA	8 cut(s)	757, 801, 1159, 1570, 1882, 2761, 3209, 3454
TaqII	GACCGA	1 cut(s)	3024
TatI	WGTACW	6 cut(s)	652, 958, 1446, 1563, 2479, 2522
TscAI	CASTG	7 cut(s)	120, 1086, 1134, 2358, 2532, 3093, 3627
TseFI	GTSAC	7 cut(s)	18, 48, 611, 3084, 3194, 3433, 3516
TseI	GCWGC	9 cut(s)	129, 1481, 1718, 2172, 2567, 3449, 3602, 3609, 3612
Tsp45I	GTSAC	7 cut(s)	18, 48, 611, 3084, 3194, 3433, 3516
TspRI	CASTG	7 cut(s)	120, 1086, 1134, 2358, 2532, 3093, 3627
Tth111I	GACNNNGTC	1 cut(s)	3469
Van91I	CCANNNNNTGG	2 cut(s)	3134, 3639
VneI	GTGCAC	1 cut(s)	432
VpaK11BI	GGWCC	2 cut(s)	313, 1712
XagI	CCTNNNNNAGG	1 cut(s)	395
XapI	RAATTY	7 cut(s)	79, 175, 264, 604, 903, 1694, 1996
XceI	RCATGY	2 cut(s)	2080, 2405
XhoI	CTCGAG	1 cut(s)	3453
XmiI	GTMKAC	2 cut(s)	1159, 2260
XmnI	GAANNNNTTC	4 cut(s)	1977, 2743, 2871, 3151
XspI	CTAG	5 cut(s)	392, 1142, 1280, 1398, 1643
ZraI	GACGTC	1 cut(s)	3194
ZrmI	AGTACT	2 cut(s)	960, 1448
Zsp2I	ATGCAT	2 cut(s)	751, 2082

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.