Rh7BG168600
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0004003
Physical Location & Seq
Forward (+)
13142043 .. 13144173
2131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG168600.1

Sequence Viewer

Length: 1308 bp
ATGCATCTTGTTAACTCAACTGCTGATGTCCTAGAGTTGATGAATATTGGCTTGGCAAATAGGGCAGTCGGTGCTACCGCCCTAAACGAAAGAAGTAGCAGATCTCACAGTGTTCTGACTGTTCATGTTCGTGGCGTGGACTTGAAGACTGACACTGCTATCCGCGGTAGCCTACACCTAGTGGATCTTGCTGGCAGCGAACGAGTGGATCGCTCTGAAGCTACCGGAGATAGGCTTAGGGAGGCACAACATATAAACAAATCATTGTCAGCCCTTGGAGATGTAATCTTTGCTCTTGCGCAAAAGAATGCACATGTGCCATATAGAAATAGCAAATTAACTCAAGTGCTCCAGAGTTCTTTAGGGGGCCAAGCAAAGACGCTCATGTTTGTACAGCTTAATCCTGATACTCAATCATTCTCTGAAACTTTGAGTACACTGAAGTTTGCAGAGAGGGTTTCTGGTGTCGAATTAGGTGCTGCAAAAAGCAACAAGGAAGGAAGATATGTAAGAGAGCTTATGGACCAGGTGGCATCGTTCAAAGAGACGATTGCCAAGAAAGATGAGGAGATTGATCGATTGCTGAAGGCCAATAGTAATGGGGTGTACCGTGGCATGAGCCCACTAAGATCTGCGTCTTCCTCTCCAAGAAGATACTCGATTGGGAGTCCTCGACAGAGAAAAGTCTCAAGCCTAGTTGATAAAGTGGCTCCATACAGGGATAGTTTGTCGGAGGCGGCATATGATAAGCATTCTGAAGTTAGTTCTCAGCTGTCAATGGATGACTTTAGACATCATAAGCAGAGGTCAAAACCTGGAGGCGATGCAAGTCAAAATATTAATGAAGGTGATGCAAGTCAAAATTTTAATGAAGGCGATGCAAGTCAAAATTTTAATGAAGACTTTGAGCTCTTGGGCTTTGGCGATGAAGATGCTGGGGAGAGATTGAGCGACATATCTGATGGTGTTCTTTCAATGGGAACAGAAACCGATGGTTCAATGTCCAGTTCTGCTGTGGAGTTTACTCTTTTTCCTGAAGTTACAAAACCAACGGAACGCACAACTACTGAAACTCCAGAAGCCAAGAAAACTCAAGCTCAGAATGCTCTAGCCGAGAAGACACCATCCCATCCGACTGTAAGGTTTGCACCAATATCTAAACTTCCAAAGCCAACACCTAAGTTGGTCGATACAAAAGCCACTCGTTCATCATTGGTTAGAAGCTCGTCAAAGGTTTTGCCGAGTCCTAGGAAAGCTACTGCTGGAAGCAGCACCTCGTCGGTGAAGTCTGGCAAACGATGGCAATAA

Protein Analysis

435

Amino Acids

47.27

Weight (kDa)

9.11

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 3 - 154 2.4e-59 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 300
AccII CGCG 1 cut(s) 165
AciI CCGC 4 cut(s) 78, 163, 165, 737
AclWI GGATC 2 cut(s) 192, 216
AcsI RAATTY 2 cut(s) 862, 889
AcuI CTGAAG 5 cut(s) 237, 461, 605, 777, 1056
AdeI CACNNNGTG 1 cut(s) 181
AfaI GTAC 3 cut(s) 393, 436, 608
AfiI CCNNNNNNNGG 1 cut(s) 231
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 4 cut(s) 145, 541, 975, 999
AjnI CCWGG 2 cut(s) 525, 814
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
AluBI AGCT 8 cut(s) 221, 397, 517, 772, 910, 1097, 1224, 1256
AluI AGCT 8 cut(s) 221, 397, 517, 772, 910, 1097, 1224, 1256
Alw21I GWGCWC 2 cut(s) 351, 912
Alw26I GTCTC 2 cut(s) 539, 691
AlwI GGATC 2 cut(s) 192, 216
AoxI GGCC 2 cut(s) 367, 588
ApeKI GCWGC 3 cut(s) 195, 479, 1269
ApoI RAATTY 2 cut(s) 862, 889
AseI ATTAAT 1 cut(s) 840
AspA2I CCTAGG 1 cut(s) 1247
AspLEI GCGC 1 cut(s) 301
AspS9I GGNCC 2 cut(s) 367, 523
AsuHPI GGTGA 2 cut(s) 860, 1294
AvaII GGWCC 1 cut(s) 523
AvrII CCTAGG 1 cut(s) 1247
BanII GRGCYC 2 cut(s) 623, 912
BbsI GAAGAC 4 cut(s) 152, 630, 906, 1124
Bbv12I GWGCWC 2 cut(s) 351, 912
BbvI GCAGC 3 cut(s) 207, 466, 1281
BccI CCATC 5 cut(s) 956, 986, 1132, 1137, 1293
BcgI CGANNNNNNTGC 4 cut(s) 458, 492, 914, 948
BciT130I CCWGG 2 cut(s) 527, 816
BcoDI GTCTC 2 cut(s) 539, 691
BfaI CTAG 5 cut(s) 32, 179, 695, 1109, 1248
BglII AGATCT 2 cut(s) 101, 629
BisI GCNGC 4 cut(s) 196, 480, 738, 1270
BlnI CCTAGG 1 cut(s) 1247
BlsI GCNGC 4 cut(s) 197, 481, 739, 1271
Bme1390I CCNGG 2 cut(s) 527, 816
Bme18I GGWCC 1 cut(s) 523
BmgT120I GGNCC 2 cut(s) 367, 523
BmiI GGNNCC 2 cut(s) 368, 711
BmrFI CCNGG 2 cut(s) 527, 816
BmsI GCATC 6 cut(s) 13, 542, 814, 841, 868, 922
BpiI GAAGAC 4 cut(s) 152, 630, 906, 1124
BpmI CTGGAG 3 cut(s) 335, 837, 1059
Bpu10I CCTNAGC 1 cut(s) 236
BpuEI CTTGAG 3 cut(s) 327, 673, 1077
Bsa29I ATCGAT 1 cut(s) 577
BsaJI CCNNGG 4 cut(s) 163, 274, 610, 1247
BsaWI WCCGGW 1 cut(s) 224
BsaXI ACNNNNNCTCC 2 cut(s) 1057, 1087
Bsc4I CCNNNNNNNGG 1 cut(s) 231
Bse1I ACTGG 1 cut(s) 1005
BseBI CCWGG 2 cut(s) 527, 816
BseCI ATCGAT 1 cut(s) 577
BseDI CCNNGG 4 cut(s) 163, 274, 610, 1247
BseGI GGATG 3 cut(s) 787, 1124, 1129
BseLI CCNNNNNNNGG 1 cut(s) 231
BseMII CTCAG 2 cut(s) 782, 1112
BseNI ACTGG 1 cut(s) 1005
BseRI GAGGAG 1 cut(s) 581
BseXI GCAGC 3 cut(s) 207, 466, 1281
BseYI CCCAGC 1 cut(s) 935
Bsh1236I CGCG 1 cut(s) 165
BshFI GGCC 2 cut(s) 369, 590
BshVI ATCGAT 1 cut(s) 577
BsiHKAI GWGCWC 2 cut(s) 351, 912
BsiSI CCGG 1 cut(s) 225
BslI CCNNNNNNNGG 1 cut(s) 231
BsmAI GTCTC 2 cut(s) 539, 691
BsmBI CGTCTC 1 cut(s) 539
BsmI GAATGC 3 cut(s) 313, 751, 1108
BsnI GGCC 2 cut(s) 369, 590
Bsp1286I GDGCHC 3 cut(s) 351, 623, 912
Bsp1407I TGTACA 1 cut(s) 391
Bsp143I GATC 5 cut(s) 101, 184, 208, 574, 629
BspACI CCGC 4 cut(s) 78, 163, 165, 737
BspANI GGCC 2 cut(s) 369, 590
BspCNI CTCAG 2 cut(s) 781, 1111
BspDI ATCGAT 1 cut(s) 577
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 2 cut(s) 368, 711
BspPI GGATC 2 cut(s) 192, 216
BsrGI TGTACA 1 cut(s) 391
BsrI ACTGG 1 cut(s) 1005
BssECI CCNNGG 4 cut(s) 163, 274, 610, 1247
BssMI GATC 5 cut(s) 101, 184, 208, 574, 629
BssT1I CCWWGG 2 cut(s) 274, 1247
Bst2UI CCWGG 2 cut(s) 527, 816
Bst4CI ACNGT 4 cut(s) 110, 121, 611, 1138
BstAPI GCANNNNNTGC 1 cut(s) 71
BstAUI TGTACA 1 cut(s) 391
BstC8I GCNNGC 1 cut(s) 193
BstDEI CTNAG 5 cut(s) 236, 626, 768, 1098, 1179
BstDSI CCRYGG 2 cut(s) 163, 610
BstF5I GGATG 3 cut(s) 787, 1124, 1129
BstFNI CGCG 1 cut(s) 165
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 5 cut(s) 104, 187, 211, 577, 632
BstMAI GTCTC 2 cut(s) 539, 691
BstMBI GATC 5 cut(s) 101, 184, 208, 574, 629
BstMWI GCNNNNNNNGC 3 cut(s) 62, 71, 1103
BstNI CCWGG 2 cut(s) 527, 816
BstNSI RCATGY 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 525, 814
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 3 cut(s) 207, 466, 1281
BstV2I GAAGAC 4 cut(s) 152, 630, 906, 1124
BstX2I RGATCY 3 cut(s) 101, 184, 629
BstYI RGATCY 3 cut(s) 101, 184, 629
Bsu15I ATCGAT 1 cut(s) 577
BsuRI GGCC 2 cut(s) 369, 590
BsuTUI ATCGAT 1 cut(s) 577
BtgI CCRYGG 2 cut(s) 163, 610
BtgZI GCGATG 3 cut(s) 837, 891, 939
BtsCI GGATG 3 cut(s) 787, 1124, 1129
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 3 cut(s) 115, 153, 437
Cac8I GCNNGC 1 cut(s) 193
CfoI GCGC 1 cut(s) 301
Cfr13I GGNCC 2 cut(s) 367, 523
Cfr42I CCGCGG 1 cut(s) 166
ClaI ATCGAT 1 cut(s) 577
CseI GACGC 2 cut(s) 388, 624
CsiI ACCWGGT 1 cut(s) 525
Csp6I GTAC 3 cut(s) 392, 435, 607
CviAII CATG 4 cut(s) 125, 314, 385, 616
CviQI GTAC 3 cut(s) 392, 435, 607
DdeI CTNAG 5 cut(s) 236, 626, 768, 1098, 1179
DpnI GATC 5 cut(s) 103, 186, 210, 576, 631
DpnII GATC 5 cut(s) 101, 184, 208, 574, 629
DraIII CACNNNGTG 1 cut(s) 181
Ecl136II GAGCTC 1 cut(s) 910
Eco130I CCWWGG 2 cut(s) 274, 1247
Eco24I GRGCYC 2 cut(s) 623, 912
Eco47I GGWCC 1 cut(s) 523
Eco53kI GAGCTC 1 cut(s) 910
Eco57I CTGAAG 5 cut(s) 237, 461, 605, 777, 1056
EcoICRI GAGCTC 1 cut(s) 910
EcoRII CCWGG 2 cut(s) 525, 814
EcoT14I CCWWGG 2 cut(s) 274, 1247
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 623, 912
ErhI CCWWGG 2 cut(s) 274, 1247
Esp3I CGTCTC 1 cut(s) 539
FaeI CATG 4 cut(s) 128, 317, 388, 619
FatI CATG 4 cut(s) 124, 313, 384, 615
FauNDI CATATG 1 cut(s) 742
Fnu4HI GCNGC 4 cut(s) 196, 480, 738, 1270
FokI GGATG 3 cut(s) 794, 1111, 1116
FriOI GRGCYC 2 cut(s) 623, 912
Fsp4HI GCNGC 4 cut(s) 196, 480, 738, 1270
FspBI CTAG 5 cut(s) 32, 179, 695, 1109, 1248
FspI TGCGCA 1 cut(s) 300
GlaI GCGC 1 cut(s) 300
GluI GCNGC 4 cut(s) 196, 480, 738, 1270
GsaI CCCAGC 1 cut(s) 939
GsuI CTGGAG 3 cut(s) 335, 837, 1059
HaeIII GGCC 2 cut(s) 369, 590
HapII CCGG 1 cut(s) 225
HgaI GACGC 2 cut(s) 388, 624
HhaI GCGC 1 cut(s) 301
Hin1II CATG 4 cut(s) 128, 317, 388, 619
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 2 cut(s) 667, 1243
HpaI GTTAAC 1 cut(s) 13
HpaII CCGG 1 cut(s) 225
HphI GGTGA 2 cut(s) 860, 1294
Hpy166II GTNNAC 5 cut(s) 13, 139, 437, 607, 1023
Hpy188I TCNGA 8 cut(s) 117, 217, 424, 733, 757, 961, 1101, 1134
Hpy188III TCNNGA 4 cut(s) 352, 404, 1034, 1076
Hpy8I GTNNAC 5 cut(s) 13, 139, 437, 607, 1023
Hpy99I CGWCG 1 cut(s) 1282
HpyAV CCTTC 4 cut(s) 491, 580, 839, 866
HpyCH4III ACNGT 4 cut(s) 110, 121, 611, 1138
HpyCH4V TGCA 8 cut(s) 4, 311, 449, 482, 827, 854, 881, 1148
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 71, 1103
HpyF3I CTNAG 5 cut(s) 236, 626, 768, 1098, 1179
Hsp92II CATG 4 cut(s) 128, 317, 388, 619
HspAI GCGC 1 cut(s) 299
KspAI GTTAAC 1 cut(s) 13
KspI CCGCGG 1 cut(s) 166
Kzo9I GATC 5 cut(s) 101, 184, 208, 574, 629
LmnI GCTCC 2 cut(s) 354, 715
Lsp1109I GCAGC 3 cut(s) 207, 466, 1281
LweI GCATC 6 cut(s) 13, 542, 814, 841, 868, 922
MabI ACCWGGT 1 cut(s) 525
MaeI CTAG 5 cut(s) 32, 179, 695, 1109, 1248
MaeIII GTNAC 1 cut(s) 1039
MalI GATC 5 cut(s) 103, 186, 210, 576, 631
MboI GATC 5 cut(s) 101, 184, 208, 574, 629
MboII GAAGA 7 cut(s) 157, 513, 630, 663, 911, 941, 1129
MflI RGATCY 3 cut(s) 101, 184, 629
MhlI GDGCHC 3 cut(s) 351, 623, 912
MluCI AATT 4 cut(s) 335, 470, 862, 889
MlyI GAGTC 2 cut(s) 676, 1252
MmeI TCCRAC 2 cut(s) 711, 1157
MnlI CCTC 9 cut(s) 235, 447, 559, 652, 681, 727, 798, 812, 1285
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 6 cut(s) 12, 338, 399, 840, 867, 894
MslI CAYNNNNRTG 1 cut(s) 129
MspA1I CMGCKG 2 cut(s) 165, 772
MspI CCGG 1 cut(s) 225
MspR9I CCNGG 2 cut(s) 527, 816
Mva1269I GAATGC 3 cut(s) 313, 751, 1108
MvaI CCWGG 2 cut(s) 527, 816
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 3 cut(s) 62, 71, 1103
NdeI CATATG 1 cut(s) 742
NdeII GATC 5 cut(s) 101, 184, 208, 574, 629
NlaIII CATG 4 cut(s) 128, 317, 388, 619
NlaIV GGNNCC 2 cut(s) 368, 711
NmeAIII GCCGAG 2 cut(s) 1138, 1266
NsbI TGCGCA 1 cut(s) 300
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 317
PciI ACATGT 1 cut(s) 313
PctI GAATGC 3 cut(s) 313, 751, 1108
PkrI GCNGC 4 cut(s) 197, 481, 739, 1271
PleI GAGTC 2 cut(s) 675, 1251
PpsI GAGTC 2 cut(s) 675, 1251
PscI ACATGT 1 cut(s) 313
PshBI ATTAAT 1 cut(s) 840
Psp124BI GAGCTC 1 cut(s) 912
Psp6I CCWGG 2 cut(s) 525, 814
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 2 cut(s) 525, 814
PspN4I GGNNCC 2 cut(s) 368, 711
PspPI GGNCC 2 cut(s) 367, 523
PsuI RGATCY 3 cut(s) 101, 184, 629
PvuII CAGCTG 1 cut(s) 772
RsaI GTAC 3 cut(s) 393, 436, 608
RsaNI GTAC 3 cut(s) 392, 435, 607
RseI CAYNNNNRTG 1 cut(s) 129
SacI GAGCTC 1 cut(s) 912
SacII CCGCGG 1 cut(s) 166
SaqAI TTAA 6 cut(s) 12, 338, 399, 840, 867, 894
SatI GCNGC 4 cut(s) 196, 480, 738, 1270
Sau3AI GATC 5 cut(s) 101, 184, 208, 574, 629
Sau96I GGNCC 2 cut(s) 367, 523
SchI GAGTC 2 cut(s) 676, 1252
ScrFI CCNGG 2 cut(s) 527, 816
SduI GDGCHC 3 cut(s) 351, 623, 912
SexAI ACCWGGT 1 cut(s) 525
SfaNI GCATC 6 cut(s) 13, 542, 814, 841, 868, 922
Sfr303I CCGCGG 1 cut(s) 166
SgrBI CCGCGG 1 cut(s) 166
SinI GGWCC 1 cut(s) 523
SmiMI CAYNNNNRTG 1 cut(s) 129
SmlI CTYRAG 3 cut(s) 342, 688, 1092
SmoI CTYRAG 3 cut(s) 342, 688, 1092
Sse9I AATT 4 cut(s) 335, 470, 862, 889
SsiI CCGC 4 cut(s) 78, 163, 165, 737
SspI AATATT 2 cut(s) 46, 838
SspMI CTAG 5 cut(s) 32, 179, 695, 1109, 1248
SstI GAGCTC 1 cut(s) 912
StyD4I CCNGG 2 cut(s) 525, 814
StyI CCWWGG 2 cut(s) 274, 1247
TaaI ACNGT 4 cut(s) 110, 121, 611, 1138
TaqI TCGA 5 cut(s) 468, 577, 659, 673, 1188
TasI AATT 4 cut(s) 335, 470, 862, 889
TatI WGTACW 2 cut(s) 391, 434
TauI GCSGC 1 cut(s) 740
Tru1I TTAA 6 cut(s) 12, 338, 399, 840, 867, 894
Tru9I TTAA 6 cut(s) 12, 338, 399, 840, 867, 894
TscAI CASTG 3 cut(s) 115, 160, 444
TseI GCWGC 3 cut(s) 195, 479, 1269
TspDTI ATGAA 7 cut(s) 56, 113, 858, 885, 912, 942, 1197
TspGWI ACGGA 1 cut(s) 1067
TspRI CASTG 3 cut(s) 115, 160, 444
VpaK11BI GGWCC 1 cut(s) 523
VspI ATTAAT 1 cut(s) 840
XapI RAATTY 2 cut(s) 862, 889
XceI RCATGY 1 cut(s) 317
XcmI CCANNNNNNNNNTGG 1 cut(s) 1012
XmaJI CCTAGG 1 cut(s) 1247
XspI CTAG 5 cut(s) 32, 179, 695, 1109, 1248
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.