RchiOBHm_Chr7g0197651
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004003
Physical Location & Seq
Forward (+)
15541844 .. 15544216
2373 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17682

Sequence Viewer

Length: 1242 bp
ATGAATATTGGCTTGGCAAATAGGGCAGTCGGTGCTACCGCCCTAAACGAAAGAAGTAGCAGATCTCACAGTGTTCTGACTGTTCATGTTCGTGGCGTGGATTTGAAGACTGACACTGCTATCCGGGGTAGCCTACACCTAGTGGATCTTGCTGGCAGCGAACGAGTGGATCGCTCTGAAGCTACCGGAGATAGGCTTAGGGAGGCACAACATATAAACAAATCATTGTCAGCCCTTGGAGATGTAATCTTTGCTCTTGCGCAAAAGAATGCACATGTGCCATATAGAAATAGCAAATTAACTCAAGTGCTCCAGAGTTCTTTAGGGGGCCAAGCGAAGACGCTCATGTTTGTACAGCTTAATCCTGATACTCAATCATTCTCTGAAACTTTGAGTACACTGAAGTTTGCAGAGAGGGTTTCTGGTGTTGAATTAGGTGCTGCAAAAAGCAACAAGGAAGGAAGATATGTAAGAGAGCTTATGGACCAGGTGGCATCGTTTAAAGAGACGATTGCCAAGAAAGATGAGGAGATTGATCGATTGCTGAAGGCCAATAGTAATGGGGTGTACCGTGGCATGAGCCCACTAAGATCTGCGTCTTCCTCTCCAAGAAGATACTCGATTGGGAGTCCTCGACAGAGGAAAGCCTCAAGCATAGTTGATAAAGTGGCTCCATATAGGGATAGTTTGTCGGAGGCGGCATACGATAAGCATTCTGAAGTTAGTTCTCAGCTGTCAATGGATGACTTTAGACATCATAAGCAGAGGTCAAAACTTGGAGGCGATGCAAGTCAAAATATCAATGAAGGCGATGCAAGTCAAAATTTTAATGAAGACTTTGAGCTCTTGGGCTTTGGCGATGAAGATACTGGGGAGAGATTGAGCGACATATCTGATGGTGTTCTTTCAATGGGAACAGAAACCGATGGTTCAATGTCCAGTTCTGCTGTGGAGTTTACTCTTTTTCCTGAAGTTACAAAACCAACAGAACGCACAACTACCGAAACTCCAGAAGCCAAGAAAACTCAAGCTCAGAATGCTCTAGGCGAGAAGACACCATCCCATCCTACTGTAAGGTTTGCACCAACATCTAAACTTCCAAAGCCAACACCGAAGTTGGTCGATACAAAAGCCACTCGTTCATCATTGGTTAGAAGCTCGTCAAAGGTTTTGCCGAGTCCTAGGAAACCTACTGTTGGAAGCAGCACCTCGTCGGTGAAGTCTGGCAAACGATGGCAATAA

Protein Analysis

413

Amino Acids

44.93

Weight (kDa)

9.47

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 1 - 141 3.3e-56 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 261
AciI CCGC 2 cut(s) 39, 698
AclWI GGATC 2 cut(s) 153, 177
AcsI RAATTY 1 cut(s) 823
AcuI CTGAAG 5 cut(s) 198, 422, 566, 738, 990
AdeI CACNNNGTG 1 cut(s) 142
AfaI GTAC 3 cut(s) 354, 397, 569
AfiI CCNNNNNNNGG 2 cut(s) 192, 1196
AflIII ACRYGT 1 cut(s) 274
AgsI TTSAA 4 cut(s) 106, 431, 909, 933
AjnI CCWGG 1 cut(s) 486
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AluBI AGCT 7 cut(s) 182, 358, 478, 733, 844, 1031, 1158
AluI AGCT 7 cut(s) 182, 358, 478, 733, 844, 1031, 1158
Alw21I GWGCWC 2 cut(s) 312, 846
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 2 cut(s) 153, 177
AoxI GGCC 2 cut(s) 328, 549
ApeKI GCWGC 3 cut(s) 156, 440, 1203
ApoI RAATTY 1 cut(s) 823
AspA2I CCTAGG 1 cut(s) 1181
AspLEI GCGC 1 cut(s) 262
AspS9I GGNCC 2 cut(s) 328, 484
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 1228
AvaII GGWCC 1 cut(s) 484
AvrII CCTAGG 1 cut(s) 1181
BanII GRGCYC 2 cut(s) 584, 846
BbsI GAAGAC 5 cut(s) 113, 344, 591, 840, 1058
Bbv12I GWGCWC 2 cut(s) 312, 846
BbvI GCAGC 3 cut(s) 168, 427, 1215
BccI CCATC 5 cut(s) 890, 920, 1066, 1071, 1227
BciT130I CCWGG 1 cut(s) 488
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 3 cut(s) 140, 1043, 1182
BglII AGATCT 2 cut(s) 62, 590
BisI GCNGC 4 cut(s) 157, 441, 699, 1204
BlnI CCTAGG 1 cut(s) 1181
BlsI GCNGC 4 cut(s) 158, 442, 700, 1205
Bme1390I CCNGG 2 cut(s) 125, 488
Bme18I GGWCC 1 cut(s) 484
BmgT120I GGNCC 2 cut(s) 328, 484
BmiI GGNNCC 2 cut(s) 329, 672
BmrFI CCNGG 2 cut(s) 125, 488
BmrI ACTGGG 1 cut(s) 879
BmsI GCATC 3 cut(s) 503, 775, 802
BmuI ACTGGG 1 cut(s) 879
BpiI GAAGAC 5 cut(s) 113, 344, 591, 840, 1058
BpmI CTGGAG 2 cut(s) 296, 993
Bpu10I CCTNAGC 1 cut(s) 197
BpuEI CTTGAG 3 cut(s) 288, 634, 1011
BpuMI CCSGG 1 cut(s) 125
Bsa29I ATCGAT 1 cut(s) 538
BsaJI CCNNGG 4 cut(s) 124, 235, 571, 1181
BsaWI WCCGGW 1 cut(s) 185
BsaXI ACNNNNNCTCC 2 cut(s) 991, 1021
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 1196
Bse1I ACTGG 2 cut(s) 874, 939
BseBI CCWGG 1 cut(s) 488
BseCI ATCGAT 1 cut(s) 538
BseDI CCNNGG 4 cut(s) 124, 235, 571, 1181
BseGI GGATG 3 cut(s) 748, 1058, 1063
BseLI CCNNNNNNNGG 2 cut(s) 192, 1196
BseMII CTCAG 2 cut(s) 743, 1046
BseNI ACTGG 2 cut(s) 874, 939
BseRI GAGGAG 1 cut(s) 542
BseXI GCAGC 3 cut(s) 168, 427, 1215
BshFI GGCC 2 cut(s) 330, 551
BshVI ATCGAT 1 cut(s) 538
BsiHKAI GWGCWC 2 cut(s) 312, 846
BsiSI CCGG 2 cut(s) 124, 186
BslI CCNNNNNNNGG 2 cut(s) 192, 1196
BsmAI GTCTC 1 cut(s) 500
BsmBI CGTCTC 1 cut(s) 500
BsmI GAATGC 3 cut(s) 274, 712, 1042
BsnI GGCC 2 cut(s) 330, 551
Bsp1286I GDGCHC 3 cut(s) 312, 584, 846
Bsp1407I TGTACA 1 cut(s) 352
Bsp143I GATC 5 cut(s) 62, 145, 169, 535, 590
BspACI CCGC 2 cut(s) 39, 698
BspANI GGCC 2 cut(s) 330, 551
BspCNI CTCAG 2 cut(s) 742, 1045
BspDI ATCGAT 1 cut(s) 538
BspLI GGNNCC 2 cut(s) 329, 672
BspPI GGATC 2 cut(s) 153, 177
BsrGI TGTACA 1 cut(s) 352
BsrI ACTGG 2 cut(s) 874, 939
BssECI CCNNGG 4 cut(s) 124, 235, 571, 1181
BssMI GATC 5 cut(s) 62, 145, 169, 535, 590
BssT1I CCWWGG 2 cut(s) 235, 1181
Bst2UI CCWGG 1 cut(s) 488
Bst4CI ACNGT 5 cut(s) 71, 82, 572, 1072, 1195
BstAPI GCANNNNNTGC 1 cut(s) 32
BstAUI TGTACA 1 cut(s) 352
BstC8I GCNNGC 1 cut(s) 154
BstDEI CTNAG 4 cut(s) 197, 587, 729, 1032
BstDSI CCRYGG 1 cut(s) 571
BstF5I GGATG 3 cut(s) 748, 1058, 1063
BstHHI GCGC 1 cut(s) 262
BstKTI GATC 5 cut(s) 65, 148, 172, 538, 593
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 5 cut(s) 62, 145, 169, 535, 590
BstMWI GCNNNNNNNGC 3 cut(s) 23, 32, 1037
BstNI CCWGG 1 cut(s) 488
BstNSI RCATGY 1 cut(s) 278
BstSCI CCNGG 2 cut(s) 123, 486
BstV1I GCAGC 3 cut(s) 168, 427, 1215
BstV2I GAAGAC 5 cut(s) 113, 344, 591, 840, 1058
BstX2I RGATCY 3 cut(s) 62, 145, 590
BstYI RGATCY 3 cut(s) 62, 145, 590
Bsu15I ATCGAT 1 cut(s) 538
BsuRI GGCC 2 cut(s) 330, 551
BsuTUI ATCGAT 1 cut(s) 538
BtgI CCRYGG 1 cut(s) 571
BtgZI GCGATG 3 cut(s) 798, 825, 873
BtsCI GGATG 3 cut(s) 748, 1058, 1063
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 3 cut(s) 76, 114, 398
Cac8I GCNNGC 1 cut(s) 154
CfoI GCGC 1 cut(s) 262
Cfr13I GGNCC 2 cut(s) 328, 484
ClaI ATCGAT 1 cut(s) 538
CseI GACGC 2 cut(s) 349, 585
CsiI ACCWGGT 1 cut(s) 486
Csp6I GTAC 3 cut(s) 353, 396, 568
CviAII CATG 4 cut(s) 86, 275, 346, 577
CviQI GTAC 3 cut(s) 353, 396, 568
DdeI CTNAG 4 cut(s) 197, 587, 729, 1032
DpnI GATC 5 cut(s) 64, 147, 171, 537, 592
DpnII GATC 5 cut(s) 62, 145, 169, 535, 590
DraI TTTAAA 1 cut(s) 502
DraIII CACNNNGTG 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 844
Eco130I CCWWGG 2 cut(s) 235, 1181
Eco24I GRGCYC 2 cut(s) 584, 846
Eco47I GGWCC 1 cut(s) 484
Eco53kI GAGCTC 1 cut(s) 844
Eco57I CTGAAG 5 cut(s) 198, 422, 566, 738, 990
EcoICRI GAGCTC 1 cut(s) 844
EcoRII CCWGG 1 cut(s) 486
EcoT14I CCWWGG 2 cut(s) 235, 1181
EcoT38I GRGCYC 2 cut(s) 584, 846
ErhI CCWWGG 2 cut(s) 235, 1181
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 4 cut(s) 89, 278, 349, 580
FatI CATG 4 cut(s) 85, 274, 345, 576
Fnu4HI GCNGC 4 cut(s) 157, 441, 699, 1204
FokI GGATG 3 cut(s) 755, 1045, 1050
FriOI GRGCYC 2 cut(s) 584, 846
Fsp4HI GCNGC 4 cut(s) 157, 441, 699, 1204
FspBI CTAG 3 cut(s) 140, 1043, 1182
FspI TGCGCA 1 cut(s) 261
GlaI GCGC 1 cut(s) 261
GluI GCNGC 4 cut(s) 157, 441, 699, 1204
GsuI CTGGAG 2 cut(s) 296, 993
HaeIII GGCC 2 cut(s) 330, 551
HapII CCGG 2 cut(s) 124, 186
HgaI GACGC 2 cut(s) 349, 585
HhaI GCGC 1 cut(s) 262
Hin1II CATG 4 cut(s) 89, 278, 349, 580
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HinfI GANTC 2 cut(s) 628, 1177
HpaII CCGG 2 cut(s) 124, 186
HphI GGTGA 1 cut(s) 1228
Hpy166II GTNNAC 3 cut(s) 398, 568, 957
Hpy188I TCNGA 7 cut(s) 78, 178, 385, 694, 718, 895, 1035
Hpy188III TCNNGA 4 cut(s) 313, 365, 968, 1010
Hpy8I GTNNAC 3 cut(s) 398, 568, 957
Hpy99I CGWCG 1 cut(s) 1216
HpyAV CCTTC 3 cut(s) 452, 541, 800
HpyCH4III ACNGT 5 cut(s) 71, 82, 572, 1072, 1195
HpyCH4V TGCA 6 cut(s) 272, 410, 443, 788, 815, 1082
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 32, 1037
HpyF3I CTNAG 4 cut(s) 197, 587, 729, 1032
Hsp92II CATG 4 cut(s) 89, 278, 349, 580
HspAI GCGC 1 cut(s) 260
Kzo9I GATC 5 cut(s) 62, 145, 169, 535, 590
LmnI GCTCC 2 cut(s) 315, 676
Lsp1109I GCAGC 3 cut(s) 168, 427, 1215
LweI GCATC 3 cut(s) 503, 775, 802
MabI ACCWGGT 1 cut(s) 486
MaeI CTAG 3 cut(s) 140, 1043, 1182
MaeIII GTNAC 1 cut(s) 973
MalI GATC 5 cut(s) 64, 147, 171, 537, 592
MboI GATC 5 cut(s) 62, 145, 169, 535, 590
MboII GAAGA 8 cut(s) 118, 349, 474, 591, 624, 845, 875, 1063
MflI RGATCY 3 cut(s) 62, 145, 590
MhlI GDGCHC 3 cut(s) 312, 584, 846
MluCI AATT 3 cut(s) 296, 431, 823
MlyI GAGTC 2 cut(s) 637, 1186
MmeI TCCRAC 2 cut(s) 672, 1177
MseI TTAA 4 cut(s) 299, 360, 501, 828
MslI CAYNNNNRTG 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 733
MspI CCGG 2 cut(s) 124, 186
MspR9I CCNGG 2 cut(s) 125, 488
Mva1269I GAATGC 3 cut(s) 274, 712, 1042
MvaI CCWGG 1 cut(s) 488
MwoI GCNNNNNNNGC 3 cut(s) 23, 32, 1037
NciI CCSGG 1 cut(s) 125
NdeII GATC 5 cut(s) 62, 145, 169, 535, 590
NlaIII CATG 4 cut(s) 89, 278, 349, 580
NlaIV GGNNCC 2 cut(s) 329, 672
NmeAIII GCCGAG 1 cut(s) 1200
NsbI TGCGCA 1 cut(s) 261
NspI RCATGY 1 cut(s) 278
PciI ACATGT 1 cut(s) 274
PctI GAATGC 3 cut(s) 274, 712, 1042
PkrI GCNGC 4 cut(s) 158, 442, 700, 1205
PleI GAGTC 2 cut(s) 636, 1185
PpsI GAGTC 2 cut(s) 636, 1185
PscI ACATGT 1 cut(s) 274
Psp124BI GAGCTC 1 cut(s) 846
Psp6I CCWGG 1 cut(s) 486
PspGI CCWGG 1 cut(s) 486
PspN4I GGNNCC 2 cut(s) 329, 672
PspPI GGNCC 2 cut(s) 328, 484
PsuI RGATCY 3 cut(s) 62, 145, 590
PvuII CAGCTG 1 cut(s) 733
RsaI GTAC 3 cut(s) 354, 397, 569
RsaNI GTAC 3 cut(s) 353, 396, 568
RseI CAYNNNNRTG 1 cut(s) 90
SacI GAGCTC 1 cut(s) 846
SaqAI TTAA 4 cut(s) 299, 360, 501, 828
SatI GCNGC 4 cut(s) 157, 441, 699, 1204
Sau3AI GATC 5 cut(s) 62, 145, 169, 535, 590
Sau96I GGNCC 2 cut(s) 328, 484
SchI GAGTC 2 cut(s) 637, 1186
ScrFI CCNGG 2 cut(s) 125, 488
SduI GDGCHC 3 cut(s) 312, 584, 846
SexAI ACCWGGT 1 cut(s) 486
SfaNI GCATC 3 cut(s) 503, 775, 802
SinI GGWCC 1 cut(s) 484
SmiMI CAYNNNNRTG 1 cut(s) 90
SmlI CTYRAG 3 cut(s) 303, 649, 1026
SmoI CTYRAG 3 cut(s) 303, 649, 1026
Sse9I AATT 3 cut(s) 296, 431, 823
SsiI CCGC 2 cut(s) 39, 698
SspI AATATT 1 cut(s) 7
SspMI CTAG 3 cut(s) 140, 1043, 1182
SstI GAGCTC 1 cut(s) 846
StyD4I CCNGG 2 cut(s) 123, 486
StyI CCWWGG 2 cut(s) 235, 1181
TaaI ACNGT 5 cut(s) 71, 82, 572, 1072, 1195
TaqI TCGA 4 cut(s) 538, 620, 634, 1122
TasI AATT 3 cut(s) 296, 431, 823
TatI WGTACW 2 cut(s) 352, 395
TauI GCSGC 1 cut(s) 701
Tru1I TTAA 4 cut(s) 299, 360, 501, 828
Tru9I TTAA 4 cut(s) 299, 360, 501, 828
TscAI CASTG 3 cut(s) 76, 121, 405
TseI GCWGC 3 cut(s) 156, 440, 1203
TspDTI ATGAA 6 cut(s) 17, 74, 819, 846, 876, 1131
TspRI CASTG 3 cut(s) 76, 121, 405
VpaK11BI GGWCC 1 cut(s) 484
XapI RAATTY 1 cut(s) 823
XceI RCATGY 1 cut(s) 278
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XmaJI CCTAGG 1 cut(s) 1181
XspI CTAG 3 cut(s) 140, 1043, 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.