MD06G1124600.v1.1

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013468
Physical Location & Seq
Reverse (-)
26682353 .. 26684200
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1124600.v1.1.491

Sequence Viewer

Length: 567 bp
ATGGCAAACCTACTAAAGAGAACATTTGGCTCTTTCATATCTTCCATTGCATGCTACTCTTACTTTAACAACCACGATCATCACCACCACCACCACCACTACCAAAGCTTCATCAACAATAAGAAAATCAAACACAGCATGCCAAGGGGTCGGCCGCTTTCGCTACAGACTGTGGAGCTCAAAGTCAGGATGTGCTGCACTGGCTGTGAAAGAGTGGTGAAAAATGCCATTTTCAAGCTCAGAGGGATTGAATCTGTGGATGTGAATCTACCATTGGAAAAGGTGACGGTAATTGGGTACATTGATCGCAACAAAGTCCTCAAGGCAGTGAGGAGGGCTGGAAAAAGGGCCGAGTTCTGGCCCTACTCCAACCCTCCATTGTACTTCACATCTTCAAATGACTATTTTAAGGACACAACCAATGAATTCAAAGAGAGCTACAACTACTATAGGCATGGCTACAATGTTGGGGACAAACATGGGACCATTCCAGTGACTCAAAGGGGAGATGACAAAGTTAGCAACATGTTCAATGATGATAATGTCCATGCGTGTTGCCTTATGTAA

Protein Analysis

189

Amino Acids

21.89

Weight (kDa)

9.36

Isoelectric Point (pI)

31.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 61 - 115 4.1e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18196 AT2G18196
fragaria_vesca FvH4_5g10380 FvH4_5g10380
malus_domestica MD06G1124600.v1.1 MD14G1141600.v1.1
prunus_persica Prupe.5G135200_v2.0.a1
pyrus_communis pycom06g11500 pycom14g11670
rosa_chinensis RchiOBHm_Chr7g0187841
rosa_laevigata RLG00000004752
rosa_multiflora Rmu_sc0004483.1_g000045
rosa_roxburghii Rroxscaffold_3G00267010
rosa_rugosa Rorug06G0484700
rosa_samantha Rh7BG091600 Rh7CG090700 Rh7DG092400
rosa_wichuraiana Rw7G007760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 155
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 1 cut(s) 425
AfaI GTAC 2 cut(s) 299, 383
AfiI CCNNNNNNNGG 1 cut(s) 357
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 5 cut(s) 235, 251, 396, 430, 532
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AluBI AGCT 4 cut(s) 108, 178, 238, 438
AluI AGCT 4 cut(s) 108, 178, 238, 438
Alw21I GWGCWC 1 cut(s) 180
AoxI GGCC 3 cut(s) 152, 348, 359
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 425
AspS9I GGNCC 3 cut(s) 348, 360, 483
AsuHPI GGTGA 3 cut(s) 74, 229, 295
AvaII GGWCC 1 cut(s) 483
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 182
BfmI CTRYAG 2 cut(s) 164, 448
BisI GCNGC 2 cut(s) 155, 196
BlsI GCNGC 2 cut(s) 156, 197
Bme18I GGWCC 1 cut(s) 483
BmgT120I GGNCC 3 cut(s) 348, 360, 483
BmiI GGNNCC 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 143
Bsc4I CCNNNNNNNGG 1 cut(s) 357
Bse1I ACTGG 2 cut(s) 205, 491
Bse3DI GCAATG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 2 cut(s) 195, 265
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 1 cut(s) 357
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 253
BseNI ACTGG 2 cut(s) 205, 491
BseRI GAGGAG 1 cut(s) 346
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 1 cut(s) 182
BsgI GTGCAG 1 cut(s) 181
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 3 cut(s) 154, 350, 361
BsiEI CGRYCG 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 180
BslFI GGGAC 2 cut(s) 485, 496
BslI CCNNNNNNNGG 1 cut(s) 357
BsmFI GGGAC 2 cut(s) 485, 496
BsnI GGCC 3 cut(s) 154, 350, 361
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 2 cut(s) 76, 304
BspACI CCGC 1 cut(s) 155
BspANI GGCC 3 cut(s) 154, 350, 361
BspCNI CTCAG 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 2 cut(s) 205, 491
BssECI CCNNGG 1 cut(s) 143
BssMI GATC 2 cut(s) 76, 304
BssT1I CCWWGG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 172, 289
BstC8I GCNNGC 2 cut(s) 52, 140
BstDEI CTNAG 1 cut(s) 239
BstF5I GGATG 2 cut(s) 195, 265
BstKTI GATC 2 cut(s) 79, 307
BstMBI GATC 2 cut(s) 76, 304
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 2 cut(s) 160, 201
BstNSI RCATGY 3 cut(s) 54, 142, 529
BstSFI CTRYAG 2 cut(s) 164, 448
BstV1I GCAGC 1 cut(s) 182
BstZI CGGCCG 1 cut(s) 152
BsuRI GGCC 3 cut(s) 154, 350, 361
BtsCI GGATG 2 cut(s) 195, 265
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 3 cut(s) 198, 333, 498
Cac8I GCNNGC 2 cut(s) 52, 140
Cfr13I GGNCC 3 cut(s) 348, 360, 483
Csp6I GTAC 2 cut(s) 298, 382
CviAII CATG 6 cut(s) 51, 139, 455, 479, 526, 548
CviQI GTAC 2 cut(s) 298, 382
DdeI CTNAG 1 cut(s) 239
DpnI GATC 2 cut(s) 78, 306
DpnII GATC 2 cut(s) 76, 304
EaeI YGGCCR 1 cut(s) 152
EagI CGGCCG 1 cut(s) 152
Ecl136II GAGCTC 1 cut(s) 178
EclXI CGGCCG 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 483
Eco52I CGGCCG 1 cut(s) 152
Eco53kI GAGCTC 1 cut(s) 178
EcoICRI GAGCTC 1 cut(s) 178
EcoRI GAATTC 1 cut(s) 425
EcoT14I CCWWGG 1 cut(s) 143
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 143
FaeI CATG 6 cut(s) 54, 142, 458, 482, 529, 551
FaiI YATR 9 cut(s) 38, 52, 140, 450, 456, 480, 527, 549, 563
FaqI GGGAC 2 cut(s) 485, 496
FatI CATG 6 cut(s) 50, 138, 454, 478, 525, 547
Fnu4HI GCNGC 2 cut(s) 155, 196
FokI GGATG 2 cut(s) 202, 272
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 2 cut(s) 155, 196
GluI GCNGC 2 cut(s) 155, 196
HaeIII GGCC 3 cut(s) 154, 350, 361
Hin1II CATG 6 cut(s) 54, 142, 458, 482, 529, 551
HindIII AAGCTT 1 cut(s) 106
HinfI GANTC 3 cut(s) 251, 265, 496
HphI GGTGA 3 cut(s) 74, 229, 295
Hpy188I TCNGA 1 cut(s) 242
Hpy188III TCNNGA 1 cut(s) 187
HpyCH4III ACNGT 2 cut(s) 172, 289
HpyCH4V TGCA 2 cut(s) 50, 198
HpyF10VI GCNNNNNNNGC 2 cut(s) 160, 201
HpyF3I CTNAG 1 cut(s) 239
Hsp92II CATG 6 cut(s) 54, 142, 458, 482, 529, 551
Kzo9I GATC 2 cut(s) 76, 304
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 5 cut(s) 172, 186, 324, 343, 504
Lsp1109I GCAGC 1 cut(s) 182
MaeIII GTNAC 2 cut(s) 283, 493
MalI GATC 2 cut(s) 78, 306
MboI GATC 2 cut(s) 76, 304
MboII GAAGA 2 cut(s) 33, 384
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 2 cut(s) 291, 425
MlyI GAGTC 1 cut(s) 490
MmeI TCCRAC 1 cut(s) 393
MnlI CCTC 5 cut(s) 236, 324, 327, 329, 384
MseI TTAA 2 cut(s) 66, 408
MslI CAYNNNNRTG 1 cut(s) 491
MwoI GCNNNNNNNGC 2 cut(s) 160, 201
NdeII GATC 2 cut(s) 76, 304
NlaIII CATG 6 cut(s) 54, 142, 458, 482, 529, 551
NlaIV GGNNCC 1 cut(s) 484
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 2 cut(s) 283, 493
NspI RCATGY 3 cut(s) 54, 142, 529
PaeI GCATGC 2 cut(s) 54, 142
PciI ACATGT 1 cut(s) 525
PfeI GAWTC 2 cut(s) 251, 265
PkrI GCNGC 2 cut(s) 156, 197
PleI GAGTC 1 cut(s) 490
PpsI GAGTC 1 cut(s) 490
PscI ACATGT 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 3 cut(s) 348, 360, 483
RsaI GTAC 2 cut(s) 299, 383
RsaNI GTAC 2 cut(s) 298, 382
RseI CAYNNNNRTG 1 cut(s) 491
SacI GAGCTC 1 cut(s) 180
SaqAI TTAA 2 cut(s) 66, 408
SatI GCNGC 2 cut(s) 155, 196
Sau3AI GATC 2 cut(s) 76, 304
Sau96I GGNCC 3 cut(s) 348, 360, 483
SchI GAGTC 1 cut(s) 490
SduI GDGCHC 1 cut(s) 180
SetI ASST 6 cut(s) 12, 110, 180, 240, 285, 440
SfcI CTRYAG 2 cut(s) 164, 448
SinI GGWCC 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SphI GCATGC 2 cut(s) 54, 142
Sse9I AATT 2 cut(s) 291, 425
SsiI CCGC 1 cut(s) 155
SstI GAGCTC 1 cut(s) 180
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 2 cut(s) 172, 289
TasI AATT 2 cut(s) 291, 425
TatI WGTACW 1 cut(s) 381
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 2 cut(s) 251, 265
Tru1I TTAA 2 cut(s) 66, 408
Tru9I TTAA 2 cut(s) 66, 408
TscAI CASTG 3 cut(s) 205, 333, 498
TseFI GTSAC 2 cut(s) 283, 493
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 2 cut(s) 283, 493
TspDTI ATGAA 3 cut(s) 25, 100, 438
TspRI CASTG 3 cut(s) 205, 333, 498
VpaK11BI GGWCC 1 cut(s) 483
XapI RAATTY 1 cut(s) 425
XceI RCATGY 3 cut(s) 54, 142, 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.