MD14G1141600.v1.1

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013468
Physical Location & Seq
Reverse (-)
23264417 .. 23266004
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1141600.v1.1.491

Sequence Viewer

Length: 567 bp
ATGGCAACCCTACTAAAGAGAACATTTGGCCCTTTCATATCTTCCATTGCATACTACTCTTACTTTAACAACCAAGATCATCACCACCACCATCACCACCACTACGAAAGCATCATAAACAAGAAAAGCAAACACAACATGCCAAGGGGTCGGCCGCTTTCACTACAGACTGTGGAGCTCATTGTCAGGATGTGCTGCACTGGCTGTGAAAGAGTTGTGAAAAATGCCATTTTCAAGCTCAGAGGGATTGATTCTGTGGATGTGGATCTACAAATGGAAAAGGTGACTGTAATCGGTTACGTTGATCGCAACAAAGTCCTCAAGGCCGTGAGGAGGGCTGGGAAAAGGGCCGAGTTTTGGCCCTACCCTAACCCTCCATTGTACTTCACATCTTCAAGTGACTATTTTAAGGACACAACCAATGAATTCAAAGAGAGCTACAACTACTATAAGCATGGCTACAATGTTGGGGACAAACATGGAAATATTCCAGTGACTCAAAGGGGAGATGACAAAATTAGCAACATGTTCAATGATGATAATGTCAATGCTTGTTGCCTTATGTAA

Protein Analysis

189

Amino Acids

21.9

Weight (kDa)

9.12

Isoelectric Point (pI)

32.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 61 - 115 1.8e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0013468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18196 AT2G18196
fragaria_vesca FvH4_5g10380 FvH4_5g10380
malus_domestica MD06G1124600.v1.1 MD14G1141600.v1.1
prunus_persica Prupe.5G135200_v2.0.a1
pyrus_communis pycom06g11500 pycom14g11670
rosa_chinensis RchiOBHm_Chr7g0187841
rosa_laevigata RLG00000004752
rosa_multiflora Rmu_sc0004483.1_g000045
rosa_roxburghii Rroxscaffold_3G00267010
rosa_rugosa Rorug06G0484700
rosa_samantha Rh7BG091600 Rh7CG090700 Rh7DG092400
rosa_wichuraiana Rw7G007760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 155
AclWI GGATC 1 cut(s) 273
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 1 cut(s) 425
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 2 cut(s) 333, 357
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 4 cut(s) 235, 396, 430, 532
AluBI AGCT 3 cut(s) 178, 238, 438
AluI AGCT 3 cut(s) 178, 238, 438
Alw21I GWGCWC 1 cut(s) 180
AlwI GGATC 1 cut(s) 273
AoxI GGCC 5 cut(s) 28, 152, 324, 348, 359
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 425
AspS9I GGNCC 3 cut(s) 29, 348, 360
AsuHPI GGTGA 3 cut(s) 74, 86, 295
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 182
BccI CCATC 1 cut(s) 99
BceAI ACGGC 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 164
BisI GCNGC 2 cut(s) 155, 196
BlsI GCNGC 2 cut(s) 156, 197
BmgT120I GGNCC 3 cut(s) 29, 348, 360
BmsI GCATC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 305
BsaBI GATNNNNATC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 143
Bsc4I CCNNNNNNNGG 2 cut(s) 333, 357
Bse1I ACTGG 2 cut(s) 205, 491
Bse3DI GCAATG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 2 cut(s) 195, 265
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 2 cut(s) 333, 357
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 253
BseNI ACTGG 2 cut(s) 205, 491
BseRI GAGGAG 1 cut(s) 346
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 1 cut(s) 182
BseYI CCCAGC 1 cut(s) 338
BsgI GTGCAG 1 cut(s) 181
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 5 cut(s) 30, 154, 326, 350, 361
BsiEI CGRYCG 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 180
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 2 cut(s) 333, 357
BsmFI GGGAC 1 cut(s) 485
BsnI GGCC 5 cut(s) 30, 154, 326, 350, 361
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 3 cut(s) 76, 265, 304
BspACI CCGC 1 cut(s) 155
BspANI GGCC 5 cut(s) 30, 154, 326, 350, 361
BspCNI CTCAG 1 cut(s) 252
BspPI GGATC 1 cut(s) 273
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 2 cut(s) 205, 491
BssECI CCNNGG 1 cut(s) 143
BssMI GATC 3 cut(s) 76, 265, 304
BssT1I CCWWGG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 172, 289
BstDEI CTNAG 1 cut(s) 239
BstF5I GGATG 2 cut(s) 195, 265
BstKTI GATC 3 cut(s) 79, 268, 307
BstMBI GATC 3 cut(s) 76, 265, 304
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 1 cut(s) 201
BstNSI RCATGY 2 cut(s) 142, 529
BstSFI CTRYAG 1 cut(s) 164
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BstZI CGGCCG 1 cut(s) 152
BsuRI GGCC 5 cut(s) 30, 154, 326, 350, 361
BtsCI GGATG 2 cut(s) 195, 265
BtsIMutI CAGTG 2 cut(s) 198, 498
Cfr13I GGNCC 3 cut(s) 29, 348, 360
Csp6I GTAC 1 cut(s) 382
CviAII CATG 4 cut(s) 139, 455, 479, 526
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 1 cut(s) 239
DpnI GATC 3 cut(s) 78, 267, 306
DpnII GATC 3 cut(s) 76, 265, 304
EaeI YGGCCR 1 cut(s) 152
EagI CGGCCG 1 cut(s) 152
Ecl136II GAGCTC 1 cut(s) 178
EclXI CGGCCG 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 180
Eco52I CGGCCG 1 cut(s) 152
Eco53kI GAGCTC 1 cut(s) 178
EcoICRI GAGCTC 1 cut(s) 178
EcoRI GAATTC 1 cut(s) 425
EcoT14I CCWWGG 1 cut(s) 143
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 143
FaeI CATG 4 cut(s) 142, 458, 482, 529
FaiI YATR 9 cut(s) 38, 52, 116, 140, 450, 456, 480, 527, 563
FaqI GGGAC 1 cut(s) 485
FatI CATG 4 cut(s) 138, 454, 478, 525
Fnu4HI GCNGC 2 cut(s) 155, 196
FokI GGATG 2 cut(s) 202, 272
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 2 cut(s) 155, 196
GluI GCNGC 2 cut(s) 155, 196
GsaI CCCAGC 1 cut(s) 342
HaeIII GGCC 5 cut(s) 30, 154, 326, 350, 361
Hin1II CATG 4 cut(s) 142, 458, 482, 529
HinfI GANTC 2 cut(s) 251, 496
HphI GGTGA 3 cut(s) 74, 86, 295
Hpy188I TCNGA 1 cut(s) 242
Hpy188III TCNNGA 1 cut(s) 187
HpyCH4III ACNGT 2 cut(s) 172, 289
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 2 cut(s) 50, 198
HpyF10VI GCNNNNNNNGC 1 cut(s) 201
HpyF3I CTNAG 1 cut(s) 239
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 4 cut(s) 142, 458, 482, 529
Kzo9I GATC 3 cut(s) 76, 265, 304
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 4 cut(s) 172, 186, 324, 504
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 1 cut(s) 120
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 4 cut(s) 283, 296, 398, 493
MalI GATC 3 cut(s) 78, 267, 306
MboI GATC 3 cut(s) 76, 265, 304
MboII GAAGA 2 cut(s) 33, 384
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 2 cut(s) 425, 516
MlyI GAGTC 1 cut(s) 490
MnlI CCTC 5 cut(s) 236, 324, 327, 329, 384
MseI TTAA 2 cut(s) 66, 408
MwoI GCNNNNNNNGC 1 cut(s) 201
NdeII GATC 3 cut(s) 76, 265, 304
NlaIII CATG 4 cut(s) 142, 458, 482, 529
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 3 cut(s) 283, 398, 493
NspI RCATGY 2 cut(s) 142, 529
PciI ACATGT 1 cut(s) 525
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 2 cut(s) 156, 197
PleI GAGTC 1 cut(s) 490
PpsI GAGTC 1 cut(s) 490
PscI ACATGT 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 338
PspPI GGNCC 3 cut(s) 29, 348, 360
PsuI RGATCY 1 cut(s) 265
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
SacI GAGCTC 1 cut(s) 180
SaqAI TTAA 2 cut(s) 66, 408
SatI GCNGC 2 cut(s) 155, 196
Sau3AI GATC 3 cut(s) 76, 265, 304
Sau96I GGNCC 3 cut(s) 29, 348, 360
SchI GAGTC 1 cut(s) 490
SduI GDGCHC 1 cut(s) 180
SetI ASST 5 cut(s) 180, 240, 285, 303, 440
SfaNI GCATC 1 cut(s) 120
SfcI CTRYAG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 2 cut(s) 425, 516
SsiI CCGC 1 cut(s) 155
SspI AATATT 1 cut(s) 487
SstI GAGCTC 1 cut(s) 180
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 2 cut(s) 172, 289
TaiI ACGT 1 cut(s) 303
TasI AATT 2 cut(s) 425, 516
TatI WGTACW 1 cut(s) 381
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 2 cut(s) 66, 408
Tru9I TTAA 2 cut(s) 66, 408
TscAI CASTG 2 cut(s) 205, 498
TseFI GTSAC 3 cut(s) 283, 398, 493
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 3 cut(s) 283, 398, 493
TspDTI ATGAA 2 cut(s) 25, 438
TspRI CASTG 2 cut(s) 205, 498
XapI RAATTY 1 cut(s) 425
XceI RCATGY 2 cut(s) 142, 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.