Rroxscaffold_3G00267010

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0013468
Physical Location & Seq
Forward (+)
60149990 .. 60151362
1373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267010.1

Sequence Viewer

Length: 483 bp
ATGCCTAGGGGTCGACCCCTTTCGTTGCAGGTTCGCTTTCATGATCCTGTAAATAATTTTGTTTTCGATCTTTCGAGTTTCGAGACTGTGGAGCTCAAGGTCAGGATGTGCTGCACTGGTTGTGAGAGAGTTGTCAAACATGCAATATTCAAGCTCAGAGGGATTGATTCGGTGGAGGTGAATCTACCAATGGAAAAGGTGACGGTAGTTGGGTATGTTGAACGGAACAAGGTACTAAAGGCGGTGAGGAGGGCTGGAAAGAGGGCTGAATTTTGGCCTTACCCCAACCCTCCCTTGTACTTCACATCCACCACTGACTATTTCAAGGACACAACCAATGAGTTCAAAGAGAGCTACAATTACTACAGGCATGGTTACAACATTGGGGACAAACATGGTACCATTCCGATGACTCAACGGGGAGACGATAAAGTCAGCAACATGTTCAACGATGATAATGTCAATGCTTGCTCTGTCATGTAA

Protein Analysis

160

Amino Acids

18.54

Weight (kDa)

8.87

Isoelectric Point (pI)

21.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 33 - 87 2.9e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18196 AT2G18196
fragaria_vesca FvH4_5g10380 FvH4_5g10380
malus_domestica MD06G1124600.v1.1 MD14G1141600.v1.1
prunus_persica Prupe.5G135200_v2.0.a1
pyrus_communis pycom06g11500 pycom14g11670
rosa_chinensis RchiOBHm_Chr7g0187841
rosa_laevigata RLG00000004752
rosa_multiflora Rmu_sc0004483.1_g000045
rosa_roxburghii Rroxscaffold_3G00267010
rosa_rugosa Rorug06G0484700
rosa_samantha Rh7BG091600 Rh7CG090700 Rh7DG092400
rosa_wichuraiana Rw7G007760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 431
Acc36I ACCTGC 1 cut(s) 19
Acc65I GGTACC 1 cut(s) 398
AccB1I GGYRCC 1 cut(s) 398
AccI GTMKAC 1 cut(s) 13
AciI CCGC 1 cut(s) 242
AclWI GGATC 1 cut(s) 38
AcsI RAATTY 1 cut(s) 269
AfaI GTAC 3 cut(s) 234, 299, 400
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 5 cut(s) 151, 221, 325, 346, 448
AluBI AGCT 3 cut(s) 94, 154, 354
AluI AGCT 3 cut(s) 94, 154, 354
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 77, 417
AlwI GGATC 1 cut(s) 38
AoxI GGCC 1 cut(s) 275
ApeKI GCWGC 1 cut(s) 111
ApoI RAATTY 1 cut(s) 269
Asp718I GGTACC 1 cut(s) 398
AspA2I CCTAGG 1 cut(s) 5
AsuHPI GGTGA 3 cut(s) 190, 211, 256
AvrII CCTAGG 1 cut(s) 5
BanI GGYRCC 1 cut(s) 398
BanII GRGCYC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 1 cut(s) 98
BcoDI GTCTC 2 cut(s) 77, 417
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 364
BfuAI ACCTGC 1 cut(s) 19
BisI GCNGC 1 cut(s) 112
BlnI CCTAGG 1 cut(s) 5
BlsI GCNGC 1 cut(s) 113
BmiI GGNNCC 1 cut(s) 400
BpuEI CTTGAG 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 5
Bse1I ACTGG 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 5
BseGI GGATG 2 cut(s) 111, 305
BseMII CTCAG 1 cut(s) 169
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 1 cut(s) 262
BseXI GCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 97
BshFI GGCC 1 cut(s) 277
BshNI GGYRCC 1 cut(s) 398
BsiHKAI GWGCWC 1 cut(s) 96
BslFI GGGAC 1 cut(s) 401
BsmAI GTCTC 2 cut(s) 77, 417
BsmBI CGTCTC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 401
BsnI GGCC 1 cut(s) 277
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 43, 67
BspACI CCGC 1 cut(s) 242
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 1 cut(s) 168
BspHI TCATGA 1 cut(s) 40
BspLI GGNNCC 1 cut(s) 400
BspMI ACCTGC 1 cut(s) 19
BspPI GGATC 1 cut(s) 38
BspT107I GGYRCC 1 cut(s) 398
BsrI ACTGG 1 cut(s) 121
BssECI CCNNGG 1 cut(s) 5
BssMI GATC 2 cut(s) 43, 67
BssT1I CCWWGG 1 cut(s) 5
Bst4CI ACNGT 2 cut(s) 88, 205
BstC8I GCNNGC 1 cut(s) 469
BstDEI CTNAG 1 cut(s) 155
BstF5I GGATG 2 cut(s) 111, 305
BstKTI GATC 2 cut(s) 46, 70
BstMAI GTCTC 2 cut(s) 77, 417
BstMBI GATC 2 cut(s) 43, 67
BstNSI RCATGY 2 cut(s) 143, 445
BstSFI CTRYAG 1 cut(s) 364
BstV1I GCAGC 1 cut(s) 98
BsuRI GGCC 1 cut(s) 277
BtsCI GGATG 2 cut(s) 111, 305
BtsIMutI CAGTG 2 cut(s) 114, 312
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 1 cut(s) 469
CciI TCATGA 1 cut(s) 40
Csp6I GTAC 3 cut(s) 233, 298, 399
CviAII CATG 6 cut(s) 41, 140, 371, 395, 442, 478
CviJI RGCY 6 cut(s) 94, 154, 254, 266, 277, 354
CviKI_1 RGCY 6 cut(s) 94, 154, 254, 266, 277, 354
CviQI GTAC 3 cut(s) 233, 298, 399
DdeI CTNAG 1 cut(s) 155
DpnI GATC 2 cut(s) 45, 69
DpnII GATC 2 cut(s) 43, 67
DrdI GACNNNNNNGTC 1 cut(s) 431
DseDI GACNNNNNNGTC 1 cut(s) 431
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 5
Eco24I GRGCYC 1 cut(s) 96
Eco53kI GAGCTC 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 94
EcoT14I CCWWGG 1 cut(s) 5
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 5
Esp3I CGTCTC 1 cut(s) 417
FaeI CATG 6 cut(s) 44, 143, 374, 398, 445, 481
FaiI YATR 7 cut(s) 42, 141, 216, 372, 396, 443, 479
FaqI GGGAC 1 cut(s) 401
FatI CATG 6 cut(s) 40, 139, 370, 394, 441, 477
FblI GTMKAC 1 cut(s) 13
Fnu4HI GCNGC 1 cut(s) 112
FokI GGATG 2 cut(s) 118, 292
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 1 cut(s) 112
FspBI CTAG 1 cut(s) 6
GluI GCNGC 1 cut(s) 112
HaeIII GGCC 1 cut(s) 277
Hin1II CATG 6 cut(s) 44, 143, 374, 398, 445, 481
HincII GTYRAC 1 cut(s) 14
HindII GTYRAC 1 cut(s) 14
HinfI GANTC 3 cut(s) 167, 181, 412
HphI GGTGA 3 cut(s) 190, 211, 256
Hpy166II GTNNAC 1 cut(s) 14
Hpy188I TCNGA 2 cut(s) 158, 408
Hpy188III TCNNGA 3 cut(s) 41, 82, 103
Hpy8I GTNNAC 1 cut(s) 14
HpyCH4III ACNGT 2 cut(s) 88, 205
HpyCH4V TGCA 3 cut(s) 28, 114, 143
HpyF3I CTNAG 1 cut(s) 155
Hsp92II CATG 6 cut(s) 44, 143, 374, 398, 445, 481
KpnI GGTACC 1 cut(s) 402
Kzo9I GATC 2 cut(s) 43, 67
LmnI GCTCC 1 cut(s) 91
LpnPI CCDG 6 cut(s) 14, 60, 88, 102, 240, 352
Lsp1109I GCAGC 1 cut(s) 98
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 2 cut(s) 199, 374
MalI GATC 2 cut(s) 45, 69
MboI GATC 2 cut(s) 43, 67
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 3 cut(s) 55, 269, 358
MlyI GAGTC 1 cut(s) 406
MnlI CCTC 6 cut(s) 152, 169, 240, 243, 255, 300
MslI CAYNNNNRTG 1 cut(s) 407
NdeII GATC 2 cut(s) 43, 67
NlaIII CATG 6 cut(s) 44, 143, 374, 398, 445, 481
NlaIV GGNNCC 1 cut(s) 400
NmuCI GTSAC 1 cut(s) 199
NspI RCATGY 2 cut(s) 143, 445
PagI TCATGA 1 cut(s) 40
PciI ACATGT 1 cut(s) 441
PfeI GAWTC 2 cut(s) 167, 181
PkrI GCNGC 1 cut(s) 113
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
PscI ACATGT 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 400
RsaI GTAC 3 cut(s) 234, 299, 400
RsaNI GTAC 3 cut(s) 233, 298, 399
RseI CAYNNNNRTG 1 cut(s) 407
SacI GAGCTC 1 cut(s) 96
SalI GTCGAC 1 cut(s) 12
SatI GCNGC 1 cut(s) 112
Sau3AI GATC 2 cut(s) 43, 67
SchI GAGTC 1 cut(s) 406
SduI GDGCHC 1 cut(s) 96
SetI ASST 8 cut(s) 33, 96, 102, 156, 180, 201, 234, 356
SfcI CTRYAG 1 cut(s) 364
SmiMI CAYNNNNRTG 1 cut(s) 407
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 3 cut(s) 55, 269, 358
SsiI CCGC 1 cut(s) 242
SspI AATATT 1 cut(s) 147
SspMI CTAG 1 cut(s) 6
SstI GAGCTC 1 cut(s) 96
StyI CCWWGG 1 cut(s) 5
TaaI ACNGT 2 cut(s) 88, 205
TaqI TCGA 4 cut(s) 13, 66, 74, 81
TasI AATT 3 cut(s) 55, 269, 358
TatI WGTACW 1 cut(s) 297
TfiI GAWTC 2 cut(s) 167, 181
TscAI CASTG 2 cut(s) 121, 319
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 1 cut(s) 111
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 1 cut(s) 29
TspGWI ACGGA 1 cut(s) 238
TspRI CASTG 2 cut(s) 121, 319
XapI RAATTY 1 cut(s) 269
XceI RCATGY 2 cut(s) 143, 445
XmaJI CCTAGG 1 cut(s) 5
XmiI GTMKAC 1 cut(s) 13
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.