Rh7BG091600

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0013468
Physical Location & Seq
Reverse (-)
6765632 .. 6766895
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG091600.1

Sequence Viewer

Length: 429 bp
ATGCCTAGGGGTCGACCCCTTTCGTTGCAGACTGTGGAGCTCAAGGTCAGGATGTGCTGCACTGGTTGTGAGAGAGTTGTCAAACATGCAATATTCAAGCTCAGAGGGATTGATTCGGTGGAGGTGAATCTACCAATGGAAAAGGTGACAGTAGTTGGGTATGTTGAACGGAACAAGGTACTAAAGGCGGTGAGGAGGGCTGGAAAGAGGGCCGAATTCTGGCCTTACCCCAACCCTCCCTTGTACTTCACATCCACCACTGACTATTTCAAGGACACAACCAATGAGTTCAAAGAGAGCTACAATTACTACAGGCATGGTTACAACATTGGGGACAAACATGGTACCATTCCGATGACTCAACGGGGAGACGATAAAGTCAGCAACATGTTCAACGATGATAATGTCAATGCATGCTCTGTCATGTAA

Protein Analysis

142

Amino Acids

16.39

Weight (kDa)

9.19

Isoelectric Point (pI)

21.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 15 - 69 2.2e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013468)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18196 AT2G18196
fragaria_vesca FvH4_5g10380 FvH4_5g10380
malus_domestica MD06G1124600.v1.1 MD14G1141600.v1.1
prunus_persica Prupe.5G135200_v2.0.a1
pyrus_communis pycom06g11500 pycom14g11670
rosa_chinensis RchiOBHm_Chr7g0187841
rosa_laevigata RLG00000004752
rosa_multiflora Rmu_sc0004483.1_g000045
rosa_roxburghii Rroxscaffold_3G00267010
rosa_rugosa Rorug06G0484700
rosa_samantha Rh7BG091600 Rh7CG090700 Rh7DG092400
rosa_wichuraiana Rw7G007760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 377
Acc65I GGTACC 1 cut(s) 344
AccB1I GGYRCC 1 cut(s) 344
AccI GTMKAC 1 cut(s) 13
AciI CCGC 1 cut(s) 188
AcsI RAATTY 1 cut(s) 215
AfaI GTAC 3 cut(s) 180, 245, 346
AfiI CCNNNNNNNGG 1 cut(s) 219
AflIII ACRYGT 1 cut(s) 387
AgsI TTSAA 5 cut(s) 97, 167, 271, 292, 394
AluBI AGCT 3 cut(s) 40, 100, 300
AluI AGCT 3 cut(s) 40, 100, 300
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 363
AoxI GGCC 2 cut(s) 210, 221
ApeKI GCWGC 1 cut(s) 57
ApoI RAATTY 1 cut(s) 215
Asp718I GGTACC 1 cut(s) 344
AspA2I CCTAGG 1 cut(s) 5
AspS9I GGNCC 1 cut(s) 210
AsuHPI GGTGA 3 cut(s) 136, 157, 202
AvrII CCTAGG 1 cut(s) 5
BanI GGYRCC 1 cut(s) 344
BanII GRGCYC 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 1 cut(s) 44
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 310
BisI GCNGC 1 cut(s) 58
BlnI CCTAGG 1 cut(s) 5
BlsI GCNGC 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 346
BpuEI CTTGAG 1 cut(s) 26
BsaJI CCNNGG 1 cut(s) 5
Bsc4I CCNNNNNNNGG 1 cut(s) 219
Bse1I ACTGG 1 cut(s) 67
BseDI CCNNGG 1 cut(s) 5
BseGI GGATG 2 cut(s) 57, 251
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMII CTCAG 1 cut(s) 115
BseNI ACTGG 1 cut(s) 67
BseRI GAGGAG 1 cut(s) 208
BseXI GCAGC 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 43
BshFI GGCC 2 cut(s) 212, 223
BshNI GGYRCC 1 cut(s) 344
BsiHKAI GWGCWC 1 cut(s) 42
BslFI GGGAC 1 cut(s) 347
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 1 cut(s) 363
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 1 cut(s) 347
BsnI GGCC 2 cut(s) 212, 223
Bsp1286I GDGCHC 1 cut(s) 42
BspACI CCGC 1 cut(s) 188
BspANI GGCC 2 cut(s) 212, 223
BspCNI CTCAG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 346
BspT107I GGYRCC 1 cut(s) 344
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 5
Bst4CI ACNGT 2 cut(s) 34, 151
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 1 cut(s) 101
BstF5I GGATG 2 cut(s) 57, 251
BstMAI GTCTC 1 cut(s) 363
BstNSI RCATGY 3 cut(s) 89, 391, 417
BstSFI CTRYAG 1 cut(s) 310
BstV1I GCAGC 1 cut(s) 44
BsuRI GGCC 2 cut(s) 212, 223
BtsCI GGATG 2 cut(s) 57, 251
BtsIMutI CAGTG 2 cut(s) 60, 258
Cac8I GCNNGC 1 cut(s) 415
Cfr13I GGNCC 1 cut(s) 210
Csp6I GTAC 3 cut(s) 179, 244, 345
CviAII CATG 6 cut(s) 86, 317, 341, 388, 414, 424
CviJI RGCY 6 cut(s) 40, 100, 200, 212, 223, 300
CviKI_1 RGCY 6 cut(s) 40, 100, 200, 212, 223, 300
CviQI GTAC 3 cut(s) 179, 244, 345
DdeI CTNAG 1 cut(s) 101
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 5
Eco24I GRGCYC 1 cut(s) 42
Eco53kI GAGCTC 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 40
EcoRI GAATTC 1 cut(s) 215
EcoT14I CCWWGG 1 cut(s) 5
EcoT22I ATGCAT 1 cut(s) 415
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 5
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 6 cut(s) 89, 320, 344, 391, 417, 427
FaiI YATR 7 cut(s) 87, 162, 318, 342, 389, 415, 425
FaqI GGGAC 1 cut(s) 347
FatI CATG 6 cut(s) 85, 316, 340, 387, 413, 423
FblI GTMKAC 1 cut(s) 13
Fnu4HI GCNGC 1 cut(s) 58
FokI GGATG 2 cut(s) 64, 238
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 1 cut(s) 58
FspBI CTAG 1 cut(s) 6
GluI GCNGC 1 cut(s) 58
HaeIII GGCC 2 cut(s) 212, 223
Hin1II CATG 6 cut(s) 89, 320, 344, 391, 417, 427
HincII GTYRAC 1 cut(s) 14
HindII GTYRAC 1 cut(s) 14
HinfI GANTC 3 cut(s) 113, 127, 358
HphI GGTGA 3 cut(s) 136, 157, 202
Hpy166II GTNNAC 1 cut(s) 14
Hpy188I TCNGA 2 cut(s) 104, 354
Hpy188III TCNNGA 1 cut(s) 49
Hpy8I GTNNAC 1 cut(s) 14
HpyCH4III ACNGT 2 cut(s) 34, 151
HpyCH4V TGCA 4 cut(s) 28, 60, 89, 413
HpyF3I CTNAG 1 cut(s) 101
Hsp92II CATG 6 cut(s) 89, 320, 344, 391, 417, 427
KpnI GGTACC 1 cut(s) 348
LmnI GCTCC 1 cut(s) 37
LpnPI CCDG 5 cut(s) 34, 48, 186, 205, 298
Lsp1109I GCAGC 1 cut(s) 44
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 2 cut(s) 145, 320
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 2 cut(s) 215, 304
MlyI GAGTC 1 cut(s) 352
MnlI CCTC 6 cut(s) 98, 115, 186, 189, 201, 246
Mph1103I ATGCAT 1 cut(s) 415
MslI CAYNNNNRTG 1 cut(s) 353
NlaIII CATG 6 cut(s) 89, 320, 344, 391, 417, 427
NlaIV GGNNCC 1 cut(s) 346
NmuCI GTSAC 1 cut(s) 145
NsiI ATGCAT 1 cut(s) 415
NspI RCATGY 3 cut(s) 89, 391, 417
PaeI GCATGC 1 cut(s) 417
PciI ACATGT 1 cut(s) 387
PfeI GAWTC 2 cut(s) 113, 127
PkrI GCNGC 1 cut(s) 59
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
PscI ACATGT 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 42
PspN4I GGNNCC 1 cut(s) 346
PspPI GGNCC 1 cut(s) 210
RsaI GTAC 3 cut(s) 180, 245, 346
RsaNI GTAC 3 cut(s) 179, 244, 345
RseI CAYNNNNRTG 1 cut(s) 353
SacI GAGCTC 1 cut(s) 42
SalI GTCGAC 1 cut(s) 12
SatI GCNGC 1 cut(s) 58
Sau96I GGNCC 1 cut(s) 210
SchI GAGTC 1 cut(s) 352
SduI GDGCHC 1 cut(s) 42
SetI ASST 7 cut(s) 42, 48, 102, 126, 147, 180, 302
SfcI CTRYAG 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SphI GCATGC 1 cut(s) 417
Sse9I AATT 2 cut(s) 215, 304
SsiI CCGC 1 cut(s) 188
SspI AATATT 1 cut(s) 93
SspMI CTAG 1 cut(s) 6
SstI GAGCTC 1 cut(s) 42
StyI CCWWGG 1 cut(s) 5
TaaI ACNGT 2 cut(s) 34, 151
TaqI TCGA 1 cut(s) 13
TasI AATT 2 cut(s) 215, 304
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 2 cut(s) 113, 127
TscAI CASTG 2 cut(s) 67, 265
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 1 cut(s) 57
Tsp45I GTSAC 1 cut(s) 145
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 2 cut(s) 67, 265
XapI RAATTY 1 cut(s) 215
XceI RCATGY 3 cut(s) 89, 391, 417
XmaJI CCTAGG 1 cut(s) 5
XmiI GTMKAC 1 cut(s) 13
XspI CTAG 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.