MD06G1137600.v1.1

DEK C terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006810
Physical Location & Seq
Reverse (-)
28275756 .. 28280301
4546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1137600.v1.1.491

Sequence Viewer

Length: 1758 bp
ATGGCGTCCGAAGCCCTAGAGGAGAAGAAACCCGAGGACGACGCTCCGGTGGCGGAGAAGCCGGAGGCTGAGGCTGAGAAGGACCTGAAGGAGGAAGCTAAGGAAGAGGAGCAAGAGAAAGAGGAAGAGAAGGAGAAGGAGAAGGTTTCGGAAGCTTCCGAGAAGGTGACTGAGAAGGAGGAGGAGAAAGTTGTTGAAGAAGGCCAAGAGGAGAAGGAGAAAGAGGGTGAGGAAACTCCGAAGAAGACTACGAGGGGTCGAAAGGCTAGCTTGAAGAAGGTGGAGAACGACCAGACCAAGTCGAAAAAAAGAGGCAGGGACTCGGCTGCGAAGAAGAGTAAAGAAGCTAAGGCGGAGAAGAAAGAGAAGGAAGCGGTGACTCCGGTGAGTGAGAGGCCCACAAGGGAGAGGAAGCTAGTTGAGCGCTACTCAGCTCCAGAGAGTGGGCGGTCTTCCGGGACTAAATCCTTTAGCATTGAGAAGGGTCACGGTACAGAACTTAAGAATATCCCAAATGTTGCTTTCAAGATGTCAAAGAGAAAAGCAGATGACAACCTTCAATTACTGCACACAATTCTTTTTGGAAAAAAAGCTAAGCCTCATAGTTTAAAGAAAAATATAAGTCAGTTTTCAGGTTATGTGTGGGTTGAGAATGAGGAAAAACATCGGGCAAGGGTAAAGGAAAAAATTGATAAATGTGTTAAAGAGAAGTTGATGGATTTTTGTGACTTACTTAATATTCCGGTTAAGGGCACTACAAAGAAGGAAGATCTTTCTGTCAAGTTATTGGAGTTCTTGGAGTCGCCACATGCTACAACTGATGCTTTGCTTGCTGAAAAGGAACAGAAAGGGCAAAAGCGGAGAAGGAAGGCTACACCGAGTAAGGCTGCAGGTTCTGGGGAAGCATCACCAGAAACATCAGCCAAGAAACAAAAAGAAACACCCAATTCTGACAAAAAGCAGGAGTCATCCAAAGCTGATGAAGAGGAGGATGATGACAAAGTTGAAACTTCAGATGTGAAGGATGACTCTGGTGAAGATGATGACCACAATATGGTTAATGAAGAAAGTGAGCCCGAAGAGAAATCTGATGAAGAGCAAAAAGAACCAAAGGACCAGGTTTCGAGTCCAAAAAGTACATCCAAAAAGACTGTAAAAGAAGGCTCAAAGGCTAAAGCTGGTAGCAAAACAACACCTTCTAAGAAGAAAACCCCAGCAAAATCTGCAAAAACTCCTACGGAATCTGCAAAGAAATCTAGTTCTACTTCGAAAAGAGGTGCAACTGATGCTGAAGGAGCTTCTGGCTCAACAACTAAAGCAAAGAGATCAGCGAAAAAACAGAAGACTGAGAAGGAAAACCCAAAGGATACCAAGGAAAAAGCCACTGGCAAAAAACAGTCAAGCAAGTCCCCAGCCAAGGCTCCAGCAAAGGATCAAGGAAAAGGGAAAGCTAGCAAGAAACCGAAGCCAGAGCCCACAAGAGATGATATGCATGCAGTAATTGTTGATATTCTGAAGGAAGTGGACTTCAATACTGCAACTTTATCAGACATTCTCAGGCAACTTGGTATCCACTTCAGTGTTGATTTGATGCATAGAAAAGCAGAGGTGAAGGCAATAATTACAGATGTAATTAACAACATGACTGATGAGGAAGATGAAGGCGAAGAAGAAGCAGAGGAGAATGCGGAAGCTGGTGATGACTCAGACAAGGATGAAGAAAAGGATGACGATGGCAAGGACAACGATGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEK_C

Protein Analysis

586

Amino Acids

64.94

Weight (kDa)

5.8

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEK_C PF08766 492 - 544 4.4e-11 DEK C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 881
AccB7I CCANNNNNTGG 2 cut(s) 443, 1054
AciI CCGC 6 cut(s) 53, 353, 374, 448, 859, 1688
AclWI GGATC 1 cut(s) 1440
AcuI CTGAAG 5 cut(s) 107, 996, 1311, 1535, 1561
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 493, 1138
AfeI AGCGCT 1 cut(s) 425
AfiI CCNNNNNNNGG 5 cut(s) 91, 443, 749, 1054, 1417
AflII CTTAAG 1 cut(s) 500
AgsI TTSAA 6 cut(s) 197, 274, 526, 560, 1007, 1531
AjnI CCWGG 1 cut(s) 1116
AleI CACNNNNGTG 1 cut(s) 1578
AlwI GGATC 1 cut(s) 1440
AlwNI CAGNNNCTG 1 cut(s) 896
Ama87I CYCGRG 1 cut(s) 32
Aor51HI AGCGCT 1 cut(s) 425
AoxI GGCC 2 cut(s) 202, 395
ApeKI GCWGC 2 cut(s) 326, 887
AspLEI GCGC 1 cut(s) 426
AspS9I GGNCC 3 cut(s) 82, 396, 1114
AsuC2I CCSGG 1 cut(s) 457
AsuHPI GGTGA 8 cut(s) 178, 239, 388, 397, 900, 1046, 1621, 1709
AsuII TTCGAA 1 cut(s) 1268
AsuNHI GCTAGC 2 cut(s) 266, 1451
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 2 cut(s) 82, 1114
BaeGI GKGCMC 1 cut(s) 755
BanII GRGCYC 2 cut(s) 1077, 1476
BarI GAAGNNNNNNTAC 2 cut(s) 856, 888
BbsI GAAGAC 3 cut(s) 251, 444, 1349
BbvCI CCTCAGC 1 cut(s) 69
BbvI GCAGC 2 cut(s) 313, 874
BccI CCATC 2 cut(s) 709, 1727
BciT130I CCWGG 1 cut(s) 1118
BciVI GTATCC 2 cut(s) 1360, 1580
BcnI CCSGG 1 cut(s) 457
BfaI CTAG 5 cut(s) 17, 267, 416, 1257, 1452
BfmI CTRYAG 1 cut(s) 888
BfoI RGCGCY 1 cut(s) 427
BfrI CTTAAG 1 cut(s) 500
BfuAI ACCTGC 1 cut(s) 881
BfuI GTATCC 2 cut(s) 1360, 1580
BglII AGATCT 1 cut(s) 769
BisI GCNGC 2 cut(s) 327, 888
BlpI GCTNAGC 1 cut(s) 594
BlsI GCNGC 2 cut(s) 328, 889
Bme1390I CCNGG 2 cut(s) 457, 1118
Bme18I GGWCC 2 cut(s) 82, 1114
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 3 cut(s) 82, 396, 1114
BmiI GGNNCC 1 cut(s) 1422
BmrFI CCNGG 2 cut(s) 457, 1118
BmsI GCATC 5 cut(s) 811, 914, 1276, 1581, 1741
BmtI GCTAGC 2 cut(s) 270, 1455
BpiI GAAGAC 3 cut(s) 251, 444, 1349
BplI GAGNNNNNCTC 2 cut(s) 413, 445
BpmI CTGGAG 2 cut(s) 420, 1407
Bpu10I CCTNAGC 3 cut(s) 69, 99, 348
Bpu1102I GCTNAGC 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 1268
BpuMI CCSGG 1 cut(s) 457
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 33, 1371, 1416
BsaWI WCCGGW 3 cut(s) 46, 382, 742
Bsc4I CCNNNNNNNGG 5 cut(s) 91, 443, 749, 1054, 1417
Bse1I ACTGG 1 cut(s) 1390
BseBI CCWGG 1 cut(s) 1118
BseDI CCNNGG 3 cut(s) 33, 1371, 1416
BseGI GGATG 6 cut(s) 968, 997, 1030, 1139, 1720, 1732
BseLI CCNNNNNNNGG 5 cut(s) 91, 443, 749, 1054, 1417
BseMII CTCAG 7 cut(s) 60, 66, 162, 444, 1338, 1570, 1719
BseNI ACTGG 1 cut(s) 1390
BseRI GAGGAG 7 cut(s) 35, 122, 194, 197, 224, 1001, 1694
BseSI GKGCMC 1 cut(s) 755
BseXI GCAGC 2 cut(s) 313, 874
BseYI CCCAGC 2 cut(s) 1213, 1411
BsgI GTGCAG 1 cut(s) 551
BshFI GGCC 2 cut(s) 204, 397
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 5 cut(s) 47, 62, 383, 456, 743
BslFI GGGAC 3 cut(s) 332, 472, 1393
BslI CCNNNNNNNGG 5 cut(s) 91, 443, 749, 1054, 1417
BsmFI GGGAC 3 cut(s) 332, 472, 1393
BsmI GAATGC 1 cut(s) 1690
BsnI GGCC 2 cut(s) 204, 397
BsoBI CYCGRG 1 cut(s) 32
Bsp119I TTCGAA 1 cut(s) 1268
Bsp1286I GDGCHC 3 cut(s) 755, 1077, 1476
Bsp143I GATC 3 cut(s) 769, 1325, 1432
Bsp1720I GCTNAGC 1 cut(s) 594
BspACI CCGC 6 cut(s) 53, 353, 374, 448, 859, 1688
BspANI GGCC 2 cut(s) 204, 397
BspCNI CTCAG 7 cut(s) 61, 67, 163, 443, 1339, 1569, 1718
BspLI GGNNCC 1 cut(s) 1422
BspMAI CTGCAG 1 cut(s) 892
BspMI ACCTGC 1 cut(s) 881
BspOI GCTAGC 2 cut(s) 270, 1455
BspPI GGATC 1 cut(s) 1440
BspQI GCTCTTC 1 cut(s) 1089
BspT104I TTCGAA 1 cut(s) 1268
BspTI CTTAAG 1 cut(s) 500
BsrI ACTGG 1 cut(s) 1390
BssECI CCNNGG 3 cut(s) 33, 1371, 1416
BssMI GATC 3 cut(s) 769, 1325, 1432
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 1371, 1416
Bst2UI CCWGG 1 cut(s) 1118
Bst4CI ACNGT 3 cut(s) 491, 1153, 1398
Bst6I CTCTTC 6 cut(s) 99, 120, 329, 978, 1074, 1089
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 500
BstAPI GCANNNNNTGC 2 cut(s) 1223, 1286
BstBI TTCGAA 1 cut(s) 1268
BstC8I GCNNGC 4 cut(s) 268, 831, 1453, 1494
BstENI CCTNNNNNAGG 1 cut(s) 89
BstF5I GGATG 6 cut(s) 968, 997, 1030, 1139, 1720, 1732
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 3 cut(s) 772, 1328, 1435
BstMBI GATC 3 cut(s) 769, 1325, 1432
BstMWI GCNNNNNNNGC 7 cut(s) 11, 50, 421, 830, 1223, 1286, 1295
BstNI CCWGG 1 cut(s) 1118
BstNSI RCATGY 2 cut(s) 812, 1496
BstSCI CCNGG 2 cut(s) 455, 1116
BstSFI CTRYAG 1 cut(s) 888
BstSLI GKGCMC 1 cut(s) 755
BstV1I GCAGC 2 cut(s) 313, 874
BstV2I GAAGAC 3 cut(s) 251, 444, 1349
BstX2I RGATCY 1 cut(s) 769
BstYI RGATCY 1 cut(s) 769
BsuI GTATCC 2 cut(s) 1360, 1580
BsuRI GGCC 2 cut(s) 204, 397
BtsCI GGATG 6 cut(s) 968, 997, 1030, 1139, 1720, 1732
BtsIMutI CAGTG 2 cut(s) 1383, 1585
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 4 cut(s) 268, 831, 1453, 1494
CaiI CAGNNNCTG 1 cut(s) 896
CfoI GCGC 1 cut(s) 426
Cfr13I GGNCC 3 cut(s) 82, 396, 1114
CseI GACGC 1 cut(s) 50
CsiI ACCWGGT 1 cut(s) 1116
Csp6I GTAC 2 cut(s) 492, 1137
CviAII CATG 3 cut(s) 809, 1493, 1642
CviQI GTAC 2 cut(s) 492, 1137
DpnI GATC 3 cut(s) 771, 1327, 1434
DpnII GATC 3 cut(s) 769, 1325, 1432
DraI TTTAAA 1 cut(s) 609
Eam1104I CTCTTC 6 cut(s) 99, 120, 329, 978, 1074, 1089
EarI CTCTTC 6 cut(s) 99, 120, 329, 978, 1074, 1089
EciI GGCGGA 2 cut(s) 68, 368
Eco130I CCWWGG 2 cut(s) 1371, 1416
Eco24I GRGCYC 2 cut(s) 1077, 1476
Eco47I GGWCC 2 cut(s) 82, 1114
Eco47III AGCGCT 1 cut(s) 425
Eco57I CTGAAG 5 cut(s) 107, 996, 1311, 1535, 1561
Eco88I CYCGRG 1 cut(s) 32
EcoNI CCTNNNNNAGG 1 cut(s) 89
EcoO109I RGGNCCY 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 1116
EcoT14I CCWWGG 2 cut(s) 1371, 1416
EcoT22I ATGCAT 2 cut(s) 1494, 1596
EcoT38I GRGCYC 2 cut(s) 1077, 1476
ErhI CCWWGG 2 cut(s) 1371, 1416
FaeI CATG 3 cut(s) 812, 1496, 1645
FaiI YATR 9 cut(s) 603, 620, 639, 810, 1055, 1490, 1494, 1596, 1643
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 3 cut(s) 332, 472, 1393
FatI CATG 3 cut(s) 808, 1492, 1641
Fnu4HI GCNGC 2 cut(s) 327, 888
FokI GGATG 6 cut(s) 955, 1004, 1037, 1126, 1727, 1739
FriOI GRGCYC 2 cut(s) 1077, 1476
Fsp4HI GCNGC 2 cut(s) 327, 888
FspBI CTAG 5 cut(s) 17, 267, 416, 1257, 1452
GlaI GCGC 1 cut(s) 425
GluI GCNGC 2 cut(s) 327, 888
GsaI CCCAGC 2 cut(s) 1217, 1415
GsuI CTGGAG 2 cut(s) 420, 1407
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 2 cut(s) 204, 397
HapII CCGG 5 cut(s) 47, 62, 383, 456, 743
HgaI GACGC 1 cut(s) 50
HhaI GCGC 1 cut(s) 426
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 812, 1496, 1645
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HindIII AAGCTT 1 cut(s) 153
HinfI GANTC 8 cut(s) 320, 379, 800, 965, 1028, 1126, 1241, 1703
HpaII CCGG 5 cut(s) 47, 62, 383, 456, 743
HphI GGTGA 8 cut(s) 178, 239, 388, 397, 900, 1046, 1621, 1709
Hpy166II GTNNAC 1 cut(s) 1525
Hpy188III TCNNGA 2 cut(s) 437, 526
Hpy8I GTNNAC 1 cut(s) 1525
Hpy99I CGWCG 1 cut(s) 44
HpyCH4III ACNGT 3 cut(s) 491, 1153, 1398
HpyCH4V TGCA 9 cut(s) 568, 890, 1226, 1247, 1280, 1492, 1496, 1538, 1594
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 50, 421, 830, 1223, 1286, 1295
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 812, 1496, 1645
HspAI GCGC 1 cut(s) 424
Kzo9I GATC 3 cut(s) 769, 1325, 1432
LguI GCTCTTC 1 cut(s) 1089
LmnI GCTCC 5 cut(s) 49, 109, 439, 1295, 1426
Lsp1109I GCAGC 2 cut(s) 313, 874
LweI GCATC 5 cut(s) 811, 914, 1276, 1581, 1741
MabI ACCWGGT 1 cut(s) 1116
MaeI CTAG 5 cut(s) 17, 267, 416, 1257, 1452
MaeIII GTNAC 4 cut(s) 166, 376, 485, 725
MalI GATC 3 cut(s) 771, 1327, 1434
MboI GATC 3 cut(s) 769, 1325, 1432
MflI RGATCY 1 cut(s) 769
MhlI GDGCHC 3 cut(s) 755, 1077, 1476
MluCI AATT 7 cut(s) 560, 573, 687, 946, 1500, 1620, 1632
MlyI GAGTC 7 cut(s) 314, 373, 809, 974, 1022, 1135, 1697
Mph1103I ATGCAT 2 cut(s) 1494, 1596
MseI TTAA 8 cut(s) 501, 608, 702, 735, 747, 1059, 1635, 1756
MslI CAYNNNNRTG 1 cut(s) 1578
MspCI CTTAAG 1 cut(s) 500
MspI CCGG 5 cut(s) 47, 62, 383, 456, 743
MspR9I CCNGG 2 cut(s) 457, 1118
Mva1269I GAATGC 1 cut(s) 1690
MvaI CCWGG 1 cut(s) 1118
MwoI GCNNNNNNNGC 7 cut(s) 11, 50, 421, 830, 1223, 1286, 1295
NciI CCSGG 1 cut(s) 457
NdeII GATC 3 cut(s) 769, 1325, 1432
NheI GCTAGC 2 cut(s) 266, 1451
NlaIII CATG 3 cut(s) 812, 1496, 1645
NlaIV GGNNCC 1 cut(s) 1422
NmeAIII GCCGAG 1 cut(s) 302
NmuCI GTSAC 4 cut(s) 166, 376, 485, 725
NsiI ATGCAT 2 cut(s) 1494, 1596
NspI RCATGY 2 cut(s) 812, 1496
NspV TTCGAA 1 cut(s) 1268
OliI CACNNNNGTG 1 cut(s) 1578
PaeI GCATGC 1 cut(s) 1496
PciSI GCTCTTC 1 cut(s) 1089
PctI GAATGC 1 cut(s) 1690
PfeI GAWTC 1 cut(s) 1241
PflFI GACNNNGTC 1 cut(s) 298
PflMI CCANNNNNTGG 2 cut(s) 443, 1054
PfoI TCCNGGA 1 cut(s) 455
PkrI GCNGC 2 cut(s) 328, 889
PleI GAGTC 7 cut(s) 314, 373, 808, 973, 1022, 1134, 1697
PpsI GAGTC 7 cut(s) 314, 373, 808, 973, 1022, 1134, 1697
PpuMI RGGWCCY 1 cut(s) 82
Psp5II RGGWCCY 1 cut(s) 82
Psp6I CCWGG 1 cut(s) 1116
PspFI CCCAGC 2 cut(s) 1213, 1411
PspGI CCWGG 1 cut(s) 1116
PspN4I GGNNCC 1 cut(s) 1422
PspPI GGNCC 3 cut(s) 82, 396, 1114
PspPPI RGGWCCY 1 cut(s) 82
PstI CTGCAG 1 cut(s) 892
PstNI CAGNNNCTG 1 cut(s) 896
PsuI RGATCY 1 cut(s) 769
PsyI GACNNNGTC 1 cut(s) 298
RsaI GTAC 2 cut(s) 493, 1138
RsaNI GTAC 2 cut(s) 492, 1137
RseI CAYNNNNRTG 1 cut(s) 1578
SapI GCTCTTC 1 cut(s) 1089
SaqAI TTAA 8 cut(s) 501, 608, 702, 735, 747, 1059, 1635, 1756
SatI GCNGC 2 cut(s) 327, 888
Sau3AI GATC 3 cut(s) 769, 1325, 1432
Sau96I GGNCC 3 cut(s) 82, 396, 1114
SchI GAGTC 7 cut(s) 314, 373, 809, 974, 1022, 1135, 1697
ScrFI CCNGG 2 cut(s) 457, 1118
SduI GDGCHC 3 cut(s) 755, 1077, 1476
SexAI ACCWGGT 1 cut(s) 1116
SfaNI GCATC 5 cut(s) 811, 914, 1276, 1581, 1741
SfcI CTRYAG 1 cut(s) 888
SfuI TTCGAA 1 cut(s) 1268
SinI GGWCC 2 cut(s) 82, 1114
SmiMI CAYNNNNRTG 1 cut(s) 1578
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
SphI GCATGC 1 cut(s) 1496
Sse9I AATT 7 cut(s) 560, 573, 687, 946, 1500, 1620, 1632
SsiI CCGC 6 cut(s) 53, 353, 374, 448, 859, 1688
SspI AATATT 1 cut(s) 739
SspMI CTAG 5 cut(s) 17, 267, 416, 1257, 1452
StyD4I CCNGG 2 cut(s) 455, 1116
StyI CCWWGG 2 cut(s) 1371, 1416
TaaI ACNGT 3 cut(s) 491, 1153, 1398
TaqI TCGA 4 cut(s) 259, 302, 1124, 1268
TasI AATT 7 cut(s) 560, 573, 687, 946, 1500, 1620, 1632
TatI WGTACW 1 cut(s) 1136
TfiI GAWTC 1 cut(s) 1241
Tru1I TTAA 8 cut(s) 501, 608, 702, 735, 747, 1059, 1635, 1756
Tru9I TTAA 8 cut(s) 501, 608, 702, 735, 747, 1059, 1635, 1756
TscAI CASTG 2 cut(s) 1390, 1585
TseFI GTSAC 4 cut(s) 166, 376, 485, 725
TseI GCWGC 2 cut(s) 326, 887
Tsp45I GTSAC 4 cut(s) 166, 376, 485, 725
TspDTI ATGAA 5 cut(s) 996, 1077, 1107, 1674, 1731
TspGWI ACGGA 1 cut(s) 1253
TspRI CASTG 2 cut(s) 1390, 1585
Tth111I GACNNNGTC 1 cut(s) 298
Van91I CCANNNNNTGG 2 cut(s) 443, 1054
Vha464I CTTAAG 1 cut(s) 500
VpaK11BI GGWCC 2 cut(s) 82, 1114
XagI CCTNNNNNAGG 1 cut(s) 89
XceI RCATGY 2 cut(s) 812, 1496
XspI CTAG 5 cut(s) 17, 267, 416, 1257, 1452
Zsp2I ATGCAT 2 cut(s) 1494, 1596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.