MD06G1139800.v1.1

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008756
Physical Location & Seq
Forward (+)
28411013 .. 28411825
813 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1139800.v1.1.491

Sequence Viewer

Length: 483 bp
ATGATTTTTGATTTAACTATTAACATGATGCTATTCTGCAGAGGAGATGATCAGTACCTTTATGTCAGTGGCGTTGGCATAAAAACTGGTGATTACTTCAACTTCTATCGTTCAGACCCCGAAACTGCATTAGCTTCATGTAACAACATCTCTTCAAGCCTGAAAAAATTGAAGGTCAATGAAACTCAAAAACATCGCTGTCACATGAGCGAAGTATTTGGGGGTTTTCTGTTCGCTTGTTGTGGCCGTGGTGAGCCATTCTTTGGACGCGCCAATGTTGATGGCTCTCCCTTTGTGGAGAACTTCTCCAGGGTTCCGTTGGCAGGAATATTTTGCGGGGGAGAAATTGCACGTGGCTCTTTGAGGTTGACCGGGGAAGCTCAGAAAGATAGTGATGCTCGTTGCACTCTGCATGTCTACTGCACCATTTATCTTGTGCTGTCATATACTCCAGCACCAGCACCAGCACCATTGGAGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FIST FIST_C

Protein Analysis

161

Amino Acids

17.67

Weight (kDa)

6.4

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FIST_C PF10442 16 - 118 1.2e-08 FIST C domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 263
AccI GTMKAC 1 cut(s) 417
AccII CGCG 1 cut(s) 270
AciI CCGC 1 cut(s) 336
AcoI YGGCCR 1 cut(s) 244
AcvI CACGTG 1 cut(s) 353
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 263, 323
AgsI TTSAA 3 cut(s) 100, 156, 172
AjnI CCWGG 1 cut(s) 308
AluBI AGCT 2 cut(s) 134, 380
AluI AGCT 2 cut(s) 134, 380
AoxI GGCC 1 cut(s) 244
AspLEI GCGC 1 cut(s) 272
AsuC2I CCSGG 1 cut(s) 373
AsuHPI GGTGA 2 cut(s) 101, 263
BbrPI CACGTG 1 cut(s) 353
BccI CCATC 1 cut(s) 275
BceAI ACGGC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 310
BclI TGATCA 1 cut(s) 49
BcnI CCSGG 1 cut(s) 373
BfmI CTRYAG 1 cut(s) 37
Bme1390I CCNGG 2 cut(s) 310, 373
BmiI GGNNCC 1 cut(s) 315
BmrFI CCNGG 2 cut(s) 310, 373
BmsI GCATC 2 cut(s) 18, 385
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 2 cut(s) 292, 435
BpuMI CCSGG 1 cut(s) 373
BsaAI YACGTR 1 cut(s) 353
BsaJI CCNNGG 3 cut(s) 247, 309, 372
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 323
Bse1I ACTGG 1 cut(s) 91
BseBI CCWGG 1 cut(s) 310
BseDI CCNNGG 3 cut(s) 247, 309, 372
BseLI CCNNNNNNNGG 2 cut(s) 263, 323
BseMII CTCAG 1 cut(s) 395
BseNI ACTGG 1 cut(s) 91
BseRI GAGGAG 1 cut(s) 57
BsgI GTGCAG 1 cut(s) 406
Bsh1236I CGCG 1 cut(s) 270
BshFI GGCC 1 cut(s) 246
BsiSI CCGG 1 cut(s) 372
BslI CCNNNNNNNGG 2 cut(s) 263, 323
BsnI GGCC 1 cut(s) 246
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 1 cut(s) 336
BspANI GGCC 1 cut(s) 246
BspCNI CTCAG 1 cut(s) 394
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 1 cut(s) 315
BspMAI CTGCAG 1 cut(s) 41
BsrI ACTGG 1 cut(s) 91
BssECI CCNNGG 3 cut(s) 247, 309, 372
BssMI GATC 1 cut(s) 49
Bst2UI CCWGG 1 cut(s) 310
Bst6I CTCTTC 1 cut(s) 157
BstBAI YACGTR 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 381
BstDSI CCRYGG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 270
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 1 cut(s) 52
BstMBI GATC 1 cut(s) 49
BstNI CCWGG 1 cut(s) 310
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 2 cut(s) 308, 371
BstSFI CTRYAG 1 cut(s) 37
BstUI CGCG 1 cut(s) 270
BsuRI GGCC 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 247
BtgZI GCGATG 1 cut(s) 179
BtsIMutI CAGTG 1 cut(s) 73
CfoI GCGC 1 cut(s) 272
CseI GACGC 1 cut(s) 276
Csp6I GTAC 1 cut(s) 55
CviAII CATG 4 cut(s) 25, 138, 205, 413
CviJI RGCY 7 cut(s) 134, 159, 246, 256, 285, 357, 380
CviKI_1 RGCY 7 cut(s) 134, 159, 246, 256, 285, 357, 380
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 1 cut(s) 381
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
EaeI YGGCCR 1 cut(s) 244
Eam1104I CTCTTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 157
Eco72I CACGTG 1 cut(s) 353
EcoRII CCWGG 1 cut(s) 308
FaeI CATG 4 cut(s) 28, 141, 208, 416
FaiI YATR 8 cut(s) 26, 63, 80, 139, 206, 414, 445, 447
FatI CATG 4 cut(s) 24, 137, 204, 412
FauI CCCGC 1 cut(s) 329
FbaI TGATCA 1 cut(s) 49
FblI GTMKAC 1 cut(s) 417
GlaI GCGC 1 cut(s) 271
GsuI CTGGAG 2 cut(s) 292, 435
HaeIII GGCC 1 cut(s) 246
HapII CCGG 1 cut(s) 372
HgaI GACGC 1 cut(s) 276
HhaI GCGC 1 cut(s) 272
Hin1II CATG 4 cut(s) 28, 141, 208, 416
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HpaII CCGG 1 cut(s) 372
HphI GGTGA 2 cut(s) 101, 263
Hpy166II GTNNAC 2 cut(s) 369, 418
Hpy188I TCNGA 2 cut(s) 115, 384
Hpy8I GTNNAC 2 cut(s) 369, 418
HpyAV CCTTC 1 cut(s) 166
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 6 cut(s) 39, 128, 350, 405, 412, 423
HpyF3I CTNAG 1 cut(s) 381
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 4 cut(s) 28, 141, 208, 416
HspAI GCGC 1 cut(s) 270
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 1 cut(s) 49
LmnI GCTCC 1 cut(s) 475
LpnPI CCDG 9 cut(s) 72, 173, 295, 309, 322, 385, 465, 471, 477
LweI GCATC 2 cut(s) 18, 385
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 2 cut(s) 140, 200
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MboII GAAGA 1 cut(s) 144
MluCI AATT 2 cut(s) 167, 345
MnlI CCTC 2 cut(s) 35, 357
MseI TTAA 2 cut(s) 14, 21
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 2 cut(s) 310, 373
MvaI CCWGG 1 cut(s) 310
MvnI CGCG 1 cut(s) 270
NciI CCSGG 1 cut(s) 373
NdeII GATC 1 cut(s) 49
NlaIII CATG 4 cut(s) 28, 141, 208, 416
NlaIV GGNNCC 1 cut(s) 315
NmuCI GTSAC 1 cut(s) 200
NspI RCATGY 1 cut(s) 416
PflMI CCANNNNNTGG 1 cut(s) 263
PmaCI CACGTG 1 cut(s) 353
PmlI CACGTG 1 cut(s) 353
Ppu21I YACGTR 1 cut(s) 353
Psp6I CCWGG 1 cut(s) 308
PspCI CACGTG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 1 cut(s) 315
PstI CTGCAG 1 cut(s) 41
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
SaqAI TTAA 2 cut(s) 14, 21
Sau3AI GATC 1 cut(s) 49
ScrFI CCNGG 2 cut(s) 310, 373
SetI ASST 6 cut(s) 60, 136, 177, 355, 368, 382
SfaNI GCATC 2 cut(s) 18, 385
SfcI CTRYAG 1 cut(s) 37
Sse9I AATT 2 cut(s) 167, 345
SsiI CCGC 1 cut(s) 336
SspI AATATT 1 cut(s) 330
StyD4I CCNGG 2 cut(s) 308, 371
TaiI ACGT 1 cut(s) 355
TasI AATT 2 cut(s) 167, 345
Tru1I TTAA 2 cut(s) 14, 21
Tru9I TTAA 2 cut(s) 14, 21
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 200
TspDTI ATGAA 2 cut(s) 126, 195
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 1 cut(s) 73
Van91I CCANNNNNTGG 1 cut(s) 263
XceI RCATGY 1 cut(s) 416
XcmI CCANNNNNNNNNTGG 1 cut(s) 316
XmiI GTMKAC 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.