Rh7BG108300

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0008756
Physical Location & Seq
Forward (+)
8178200 .. 8182624
4425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG108300.1

Sequence Viewer

Length: 1299 bp
ATGAAAGTTTGTGAGTGTGTTTATGGTTACGGATTGAGATTGCAGGTAAAGGAGAGATTTGGGTCCAGAGCTCCGTTTGTTGTTTCTACTGCAAATGGGATTCTTGGAAGAGATGTTGTTACCTGTGAAATCAAAGAGGTACAGTGGGACAGTGTTTCTAGTGATGTGAGTGATGATGATGGCTCCGTACCTGCAGAGGATGGGAGCTACGGCATTGTTTTGACTGTCGGCTTTGTGCCGGGTTTGAAAGTTGATGCTATCCCGCTGTTATCGACAAGTGTAAAGGAATGTCAAGAGGTCTTTATCGATAAGTTTGTCATGGATATTAAGGATTACACAGCTTCTGTTTCAGATTGCACAGCCCCAGTAGGGATTATAATGTTTGGAGATGGGTATACTAATATGAGACCAGTAGTTGATGCATTGGATTATGTTATGCCCGAGGAAACTGTCATCGTGGGTGACGAGAAGGGTCGGTTTTTGTATAGAAGTGAGAATGAGTCTAGAAATGTCTGTGGGACTGCAACATCCTTTTCAGTTGCTGTTGCTCTTATATTTGCCAGGGACAGAGAAAACTCCTGTGGGGATATTCAATTCCAAATTGCATTGTCAGAAGGTGTGTCAACAGTAGGTCCCAAACACAAGGCTGTCTCTGTTAGAGCTAGCGCCTCTGATTCTACTTGGCTCACTGCTAGAAGAGAAGGACACCGCGAGATTCTTGATGGTCAACGAATTCTGAATGATATTAACGATGAGTTAGAAAATCACACGGATTCTTCTGATCTGTACATTGGGGTTAAAAAACGAAGAAGTATCTCCACAGGGCCAAAGACGCGTAGGCTAGTTACTTCCCTGGACTTCCATGGAGTTATAGGTGGAGACGAATCGTACCTTTATGTCAATGATGTTGGCATCAAAACTGGTGATTACTTCCATTTCTATCGCCCAGATCCCCAAACTGCCTTATCCTCGTGTACAAATCTCTCTGTAAGCCTGAAAAACTTGAAGATAGATGAAAATTCAAAAGATTGCTGTAACCTAGGTGAAGTATTTGGGGGTTTCATCTTCGCTTGTTGTGGCCGCGGTGAGTCATTCTTTGAACGTGTGAATGTGGATAGCTCGCCTTTTGTTGAAAACTTCCCTCGGATTCCATTGGGAGGAATATTTTGTGGGGGTGAAATTGGACGTGGCTCTTCAATGTTTACCAGGGGAGCTCAGAAGGATTGCAAATGCAATCTGCATGTCTATAGTACTATTTATCTTGTGTTGTCATGTACTCCAGCAGCATTAGATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FIST FIST_C

Protein Analysis

432

Amino Acids

47.24

Weight (kDa)

5.02

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FIST_C PF10442 305 - 396 6.4e-07 FIST C domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 377
Acc36I ACCTGC 2 cut(s) 34, 199
AccI GTMKAC 1 cut(s) 395
AccII CGCG 3 cut(s) 711, 835, 1083
AciI CCGC 4 cut(s) 263, 709, 1081, 1083
AclWI GGATC 1 cut(s) 944
AcoI YGGCCR 1 cut(s) 1078
AcsI RAATTY 2 cut(s) 732, 1018
AfaI GTAC 7 cut(s) 141, 189, 788, 890, 976, 1252, 1276
AfiI CCNNNNNNNGG 2 cut(s) 369, 1157
AflIII ACRYGT 2 cut(s) 833, 1102
AgsI TTSAA 7 cut(s) 247, 593, 1006, 1023, 1100, 1133, 1197
AjiI CACGTC 1 cut(s) 1187
AjnI CCWGG 3 cut(s) 560, 852, 1205
AluBI AGCT 6 cut(s) 71, 207, 341, 662, 1119, 1214
AluI AGCT 6 cut(s) 71, 207, 341, 662, 1119, 1214
Alw21I GWGCWC 2 cut(s) 73, 1216
Alw26I GTCTC 3 cut(s) 400, 655, 873
AlwI GGATC 1 cut(s) 944
AlwNI CAGNNNCTG 2 cut(s) 344, 542
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 2 cut(s) 824, 1078
ApeKI GCWGC 1 cut(s) 1283
ApoI RAATTY 2 cut(s) 732, 1018
AspA2I CCTAGG 1 cut(s) 1039
AspLEI GCGC 1 cut(s) 668
AspS9I GGNCC 3 cut(s) 63, 632, 824
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 5 cut(s) 473, 935, 1055, 1097, 1187
AsuNHI GCTAGC 1 cut(s) 662
AvaI CYCGRG 1 cut(s) 440
AvaII GGWCC 2 cut(s) 63, 632
AvrII CCTAGG 1 cut(s) 1039
BanII GRGCYC 2 cut(s) 73, 1216
BauI CACGAG 1 cut(s) 970
Bbv12I GWGCWC 2 cut(s) 73, 1216
BbvI GCAGC 1 cut(s) 1295
BccI CCATC 4 cut(s) 173, 194, 383, 716
BceAI ACGGC 1 cut(s) 226
BciT130I CCWGG 3 cut(s) 562, 854, 1207
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 3 cut(s) 400, 655, 873
BfaI CTAG 6 cut(s) 159, 504, 663, 693, 842, 1040
BfmI CTRYAG 2 cut(s) 192, 1246
BfoI RGCGCY 1 cut(s) 669
BfuAI ACCTGC 2 cut(s) 34, 199
BisI GCNGC 2 cut(s) 1081, 1284
BlnI CCTAGG 1 cut(s) 1039
BlsI GCNGC 2 cut(s) 1082, 1285
BmcAI AGTACT 1 cut(s) 1252
Bme1390I CCNGG 4 cut(s) 240, 562, 854, 1207
Bme18I GGWCC 2 cut(s) 63, 632
BmeT110I CYCGRG 1 cut(s) 440
BmgBI CACGTC 1 cut(s) 1187
BmgT120I GGNCC 3 cut(s) 63, 632, 824
BmiI GGNNCC 3 cut(s) 64, 184, 634
BmrFI CCNGG 4 cut(s) 240, 562, 854, 1207
BmrI ACTGGG 1 cut(s) 359
BmsI GCATC 3 cut(s) 244, 409, 921
BmtI GCTAGC 1 cut(s) 666
BmuI ACTGGG 1 cut(s) 359
BpmI CTGGAG 1 cut(s) 1263
BpuMI CCSGG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 400
BsaJI CCNNGG 8 cut(s) 441, 561, 852, 862, 1039, 1081, 1142, 1206
Bsc4I CCNNNNNNNGG 2 cut(s) 369, 1157
Bse1I ACTGG 3 cut(s) 365, 410, 925
BseBI CCWGG 3 cut(s) 562, 854, 1207
BseCI ATCGAT 1 cut(s) 306
BseDI CCNNGG 8 cut(s) 441, 561, 852, 862, 1039, 1081, 1142, 1206
BseGI GGATG 2 cut(s) 205, 527
BseLI CCNNNNNNNGG 2 cut(s) 369, 1157
BseMII CTCAG 1 cut(s) 1229
BseNI ACTGG 3 cut(s) 365, 410, 925
BseXI GCAGC 1 cut(s) 1295
Bsh1236I CGCG 3 cut(s) 711, 835, 1083
BshFI GGCC 2 cut(s) 826, 1080
BshVI ATCGAT 1 cut(s) 306
BsiHKAI GWGCWC 2 cut(s) 73, 1216
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 1 cut(s) 239
BslFI GGGAC 4 cut(s) 161, 532, 578, 618
BslI CCNNNNNNNGG 2 cut(s) 369, 1157
BsmAI GTCTC 3 cut(s) 400, 655, 873
BsmBI CGTCTC 1 cut(s) 873
BsmFI GGGAC 4 cut(s) 161, 532, 578, 618
BsnI GGCC 2 cut(s) 826, 1080
Bso31I GGTCTC 1 cut(s) 400
BsoBI CYCGRG 1 cut(s) 440
Bsp1286I GDGCHC 2 cut(s) 73, 1216
Bsp1407I TGTACA 2 cut(s) 786, 974
Bsp143I GATC 3 cut(s) 781, 949, 1291
Bsp19I CCATGG 1 cut(s) 862
BspACI CCGC 4 cut(s) 263, 709, 1081, 1083
BspANI GGCC 2 cut(s) 826, 1080
BspCNI CTCAG 1 cut(s) 1228
BspDI ATCGAT 1 cut(s) 306
BspFNI CGCG 3 cut(s) 711, 835, 1083
BspLI GGNNCC 3 cut(s) 64, 184, 634
BspMAI CTGCAG 1 cut(s) 196
BspMI ACCTGC 2 cut(s) 34, 199
BspOI GCTAGC 1 cut(s) 666
BspPI GGATC 1 cut(s) 944
BspQI GCTCTTC 1 cut(s) 1198
BspTNI GGTCTC 1 cut(s) 400
BsrGI TGTACA 2 cut(s) 786, 974
BsrI ACTGG 3 cut(s) 365, 410, 925
BssECI CCNNGG 8 cut(s) 441, 561, 852, 862, 1039, 1081, 1142, 1206
BssMI GATC 3 cut(s) 781, 949, 1291
BssNAI GTATAC 1 cut(s) 396
BssSI CACGAG 1 cut(s) 970
BssT1I CCWWGG 2 cut(s) 862, 1039
Bst1107I GTATAC 1 cut(s) 396
Bst2BI CACGAG 1 cut(s) 970
Bst2UI CCWGG 3 cut(s) 562, 854, 1207
Bst4CI ACNGT 5 cut(s) 144, 152, 226, 451, 628
Bst6I CTCTTC 3 cut(s) 103, 691, 1198
BstAUI TGTACA 2 cut(s) 786, 974
BstC8I GCNNGC 2 cut(s) 664, 1121
BstDEI CTNAG 1 cut(s) 1215
BstDSI CCRYGG 2 cut(s) 862, 1081
BstF5I GGATG 2 cut(s) 205, 527
BstFNI CGCG 3 cut(s) 711, 835, 1083
BstH2I RGCGCY 1 cut(s) 669
BstHHI GCGC 1 cut(s) 668
BstKTI GATC 3 cut(s) 784, 952, 1294
BstMAI GTCTC 3 cut(s) 400, 655, 873
BstMBI GATC 3 cut(s) 781, 949, 1291
BstMWI GCNNNNNNNGC 1 cut(s) 832
BstNI CCWGG 3 cut(s) 562, 854, 1207
BstNSI RCATGY 1 cut(s) 1244
BstSCI CCNGG 4 cut(s) 238, 560, 852, 1205
BstSFI CTRYAG 2 cut(s) 192, 1246
BstUI CGCG 3 cut(s) 711, 835, 1083
BstV1I GCAGC 1 cut(s) 1295
BstX2I RGATCY 1 cut(s) 949
BstYI RGATCY 1 cut(s) 949
BstZ17I GTATAC 1 cut(s) 396
Bsu15I ATCGAT 1 cut(s) 306
BsuRI GGCC 2 cut(s) 826, 1080
BsuTUI ATCGAT 1 cut(s) 306
BtgI CCRYGG 2 cut(s) 862, 1081
BtrI CACGTC 1 cut(s) 1187
BtsCI GGATG 2 cut(s) 205, 527
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 3 cut(s) 149, 157, 687
BveI ACCTGC 2 cut(s) 34, 199
Cac8I GCNNGC 2 cut(s) 664, 1121
CaiI CAGNNNCTG 2 cut(s) 344, 542
CfoI GCGC 1 cut(s) 668
Cfr13I GGNCC 3 cut(s) 63, 632, 824
Cfr42I CCGCGG 1 cut(s) 1084
ClaI ATCGAT 1 cut(s) 306
CseI GACGC 1 cut(s) 841
Csp6I GTAC 7 cut(s) 140, 188, 787, 889, 975, 1251, 1275
CviAII CATG 4 cut(s) 319, 863, 1241, 1272
CviQI GTAC 7 cut(s) 140, 188, 787, 889, 975, 1251, 1275
DdeI CTNAG 1 cut(s) 1215
DpnI GATC 3 cut(s) 783, 951, 1293
DpnII GATC 3 cut(s) 781, 949, 1291
EaeI YGGCCR 1 cut(s) 1078
Eam1104I CTCTTC 3 cut(s) 103, 691, 1198
EarI CTCTTC 3 cut(s) 103, 691, 1198
Ecl136II GAGCTC 2 cut(s) 71, 1214
Eco130I CCWWGG 2 cut(s) 862, 1039
Eco24I GRGCYC 2 cut(s) 73, 1216
Eco31I GGTCTC 1 cut(s) 400
Eco47I GGWCC 2 cut(s) 63, 632
Eco53kI GAGCTC 2 cut(s) 71, 1214
Eco88I CYCGRG 1 cut(s) 440
EcoICRI GAGCTC 2 cut(s) 71, 1214
EcoO109I RGGNCCY 1 cut(s) 632
EcoRI GAATTC 1 cut(s) 732
EcoRII CCWGG 3 cut(s) 560, 852, 1205
EcoT14I CCWWGG 2 cut(s) 862, 1039
EcoT22I ATGCAT 1 cut(s) 424
EcoT38I GRGCYC 2 cut(s) 73, 1216
ErhI CCWWGG 2 cut(s) 862, 1039
Esp3I CGTCTC 1 cut(s) 873
FaeI CATG 4 cut(s) 322, 866, 1244, 1275
FaqI GGGAC 4 cut(s) 161, 532, 578, 618
FatI CATG 4 cut(s) 318, 862, 1240, 1271
FauI CCCGC 1 cut(s) 270
FblI GTMKAC 1 cut(s) 395
Fnu4HI GCNGC 2 cut(s) 1081, 1284
FokI GGATG 2 cut(s) 212, 514
FriOI GRGCYC 2 cut(s) 73, 1216
Fsp4HI GCNGC 2 cut(s) 1081, 1284
FspBI CTAG 6 cut(s) 159, 504, 663, 693, 842, 1040
GlaI GCGC 1 cut(s) 667
GluI GCNGC 2 cut(s) 1081, 1284
GsuI CTGGAG 1 cut(s) 1263
HaeII RGCGCY 1 cut(s) 669
HaeIII GGCC 2 cut(s) 826, 1080
HapII CCGG 1 cut(s) 239
HgaI GACGC 1 cut(s) 841
HhaI GCGC 1 cut(s) 668
Hin1II CATG 4 cut(s) 322, 866, 1244, 1275
Hin6I GCGC 1 cut(s) 666
HinP1I GCGC 1 cut(s) 666
HincII GTYRAC 2 cut(s) 624, 728
HindII GTYRAC 2 cut(s) 624, 728
HinfI GANTC 8 cut(s) 100, 500, 674, 715, 773, 884, 1088, 1147
HpaII CCGG 1 cut(s) 239
HphI GGTGA 5 cut(s) 473, 935, 1055, 1097, 1187
Hpy166II GTNNAC 5 cut(s) 396, 624, 728, 975, 1203
Hpy188I TCNGA 7 cut(s) 352, 613, 673, 738, 781, 1146, 1218
Hpy188III TCNNGA 4 cut(s) 66, 293, 504, 719
Hpy8I GTNNAC 5 cut(s) 396, 624, 728, 975, 1203
HpyAV CCTTC 4 cut(s) 463, 608, 695, 1213
HpyCH4III ACNGT 5 cut(s) 144, 152, 226, 451, 628
HpyCH4IV ACGT 2 cut(s) 1102, 1186
HpyF10VI GCNNNNNNNGC 1 cut(s) 832
HpyF3I CTNAG 1 cut(s) 1215
HpySE526I ACGT 2 cut(s) 1102, 1186
Hsp92II CATG 4 cut(s) 322, 866, 1244, 1275
HspAI GCGC 1 cut(s) 666
KspI CCGCGG 1 cut(s) 1084
Kzo9I GATC 3 cut(s) 781, 949, 1291
LguI GCTCTTC 1 cut(s) 1198
LmnI GCTCC 4 cut(s) 76, 188, 204, 1211
Lsp1109I GCAGC 1 cut(s) 1295
LweI GCATC 3 cut(s) 244, 409, 921
MaeI CTAG 6 cut(s) 159, 504, 663, 693, 842, 1040
MaeII ACGT 2 cut(s) 1102, 1186
MaeIII GTNAC 5 cut(s) 26, 118, 461, 844, 1034
MalI GATC 3 cut(s) 783, 951, 1293
MboI GATC 3 cut(s) 781, 949, 1291
MboII GAAGA 7 cut(s) 120, 708, 768, 819, 1018, 1057, 1185
MflI RGATCY 1 cut(s) 949
MhlI GDGCHC 2 cut(s) 73, 1216
MluCI AATT 5 cut(s) 593, 600, 732, 1018, 1179
MluI ACGCGT 1 cut(s) 833
MlyI GAGTC 2 cut(s) 509, 1097
MnlI CCTC 8 cut(s) 130, 190, 289, 436, 679, 979, 1151, 1152
Mph1103I ATGCAT 1 cut(s) 424
MseI TTAA 3 cut(s) 327, 747, 798
MspA1I CMGCKG 2 cut(s) 265, 1083
MspI CCGG 1 cut(s) 239
MspR9I CCNGG 4 cut(s) 240, 562, 854, 1207
MvaI CCWGG 3 cut(s) 562, 854, 1207
MvnI CGCG 3 cut(s) 711, 835, 1083
MwoI GCNNNNNNNGC 1 cut(s) 832
NciI CCSGG 1 cut(s) 240
NcoI CCATGG 1 cut(s) 862
NdeII GATC 3 cut(s) 781, 949, 1291
NheI GCTAGC 1 cut(s) 662
NlaIII CATG 4 cut(s) 322, 866, 1244, 1275
NlaIV GGNNCC 3 cut(s) 64, 184, 634
NmuCI GTSAC 1 cut(s) 461
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 1 cut(s) 1244
PciSI GCTCTTC 1 cut(s) 1198
PcsI WCGNNNNNNNCGW 1 cut(s) 462
PfeI GAWTC 6 cut(s) 100, 674, 715, 773, 884, 1147
PkrI GCNGC 2 cut(s) 1082, 1285
PleI GAGTC 2 cut(s) 508, 1096
PpsI GAGTC 2 cut(s) 508, 1096
PpuMI RGGWCCY 1 cut(s) 632
PsiI TTATAA 1 cut(s) 377
Psp124BI GAGCTC 2 cut(s) 73, 1216
Psp5II RGGWCCY 1 cut(s) 632
Psp6I CCWGG 3 cut(s) 560, 852, 1205
PspGI CCWGG 3 cut(s) 560, 852, 1205
PspN4I GGNNCC 3 cut(s) 64, 184, 634
PspPI GGNCC 3 cut(s) 63, 632, 824
PspPPI RGGWCCY 1 cut(s) 632
PstI CTGCAG 1 cut(s) 196
PstNI CAGNNNCTG 2 cut(s) 344, 542
PsuI RGATCY 1 cut(s) 949
RsaI GTAC 7 cut(s) 141, 189, 788, 890, 976, 1252, 1276
RsaNI GTAC 7 cut(s) 140, 188, 787, 889, 975, 1251, 1275
SacI GAGCTC 2 cut(s) 73, 1216
SacII CCGCGG 1 cut(s) 1084
SapI GCTCTTC 1 cut(s) 1198
SaqAI TTAA 3 cut(s) 327, 747, 798
SatI GCNGC 2 cut(s) 1081, 1284
Sau3AI GATC 3 cut(s) 781, 949, 1291
Sau96I GGNCC 3 cut(s) 63, 632, 824
ScaI AGTACT 1 cut(s) 1252
SchI GAGTC 2 cut(s) 509, 1097
ScrFI CCNGG 4 cut(s) 240, 562, 854, 1207
SduI GDGCHC 2 cut(s) 73, 1216
SfaNI GCATC 3 cut(s) 244, 409, 921
SfcI CTRYAG 2 cut(s) 192, 1246
Sfr303I CCGCGG 1 cut(s) 1084
SgrBI CCGCGG 1 cut(s) 1084
SinI GGWCC 2 cut(s) 63, 632
Sse9I AATT 5 cut(s) 593, 600, 732, 1018, 1179
SsiI CCGC 4 cut(s) 263, 709, 1081, 1083
SspI AATATT 1 cut(s) 1164
SspMI CTAG 6 cut(s) 159, 504, 663, 693, 842, 1040
SstI GAGCTC 2 cut(s) 73, 1216
StyD4I CCNGG 4 cut(s) 238, 560, 852, 1205
StyI CCWWGG 2 cut(s) 862, 1039
TaaI ACNGT 5 cut(s) 144, 152, 226, 451, 628
TaiI ACGT 2 cut(s) 1105, 1189
TaqI TCGA 2 cut(s) 272, 306
TasI AATT 5 cut(s) 593, 600, 732, 1018, 1179
TatI WGTACW 4 cut(s) 786, 974, 1250, 1274
TauI GCSGC 1 cut(s) 1083
TfiI GAWTC 6 cut(s) 100, 674, 715, 773, 884, 1147
Tru1I TTAA 3 cut(s) 327, 747, 798
Tru9I TTAA 3 cut(s) 327, 747, 798
TscAI CASTG 3 cut(s) 149, 157, 694
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 1 cut(s) 1283
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 3 cut(s) 17, 1029, 1051
TspGWI ACGGA 4 cut(s) 45, 63, 175, 785
TspRI CASTG 3 cut(s) 149, 157, 694
VpaK11BI GGWCC 2 cut(s) 63, 632
XapI RAATTY 2 cut(s) 732, 1018
XbaI TCTAGA 1 cut(s) 503
XceI RCATGY 1 cut(s) 1244
XmaJI CCTAGG 1 cut(s) 1039
XmiI GTMKAC 1 cut(s) 395
XspI CTAG 6 cut(s) 159, 504, 663, 693, 842, 1040
ZrmI AGTACT 1 cut(s) 1252
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.