pycom06g13010

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008756
Physical Location & Seq
Forward (+)
18102315 .. 18106121
3807 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g13010.8

Sequence Viewer

Length: 1293 bp
ATGGACGGGGCATCGTCGTCGGCGGCTGCGGCGATAAAAACCGACACAAAACGGCCCCAAACGGCGGCGGCAGGGTTGTTTTCGCCGATAAACGACGACCTTCTCCACAAAATCCTCTCGCGCCTACCCGCCTTATCCTTCGCATCAGCGGCGTGCGTTAGCAAATCCTGGAACCAAATCTGCAGCCGAATCCTCACTCGCCCGAAGCTCGCCTCCGCCCTCTCTCTCCACCCCTCGCCTAAGGTTGCGGTGAAGGAGGCTATCGAAAAGGTTCTAGCTGAGCCGATACGGCCTCATTTTGTTGTAGCTAATATTGGAAGTGGATTTGGATTGTATGACATTTCCAAGCTTATATCGAAAAAATTGGGGTCCAGTGTTCCATTCATCATTTCTACTTCAAGTGGAATATTTGGAAGAGATGCTCTTACCCATGAATTCAAAGAGGTTAAGTGGGGAGAGGTTTGTGGTGATGGAATTGATGAAGATTGCTCTATACCAGCAAAGGATGCGAACTACGGCATCCTTTTGACTGTTGGCTTTGTGCCAGGATTGAAAGTTGATGCCATCCCGCTGTTACGAACAATAAAGCAGGATCCTCGAGAAGTCTTGCTTGATAAATTCATCATGGATATTAAGGATTACACAGCCTCAGTTTCAGATTGCACGTCCCCGGTTGGAATTATGATATTTGGAGATGGCCTTCGTGACATGAAACCGATTGTTGATGCGTTGGATTATGCTATGCCTGCGGAAACTGTCATTGTGGGTGATGAGAGAGGTCGCTTTTTATATAGAAGTGGGAACGAGTCTAGAAATGTCTGTGGGAGTGCAAAATACTTTACCGATGCTGTTACTCTTGTATTTGCCAAGGATAAGGACAAGCCACATGGTATTGGAGACATTCAATTCCAAATTGCATTATCAAATGGAGTGTCCACAGTCGGTTCCCGGCACAAGGCTGTTTCTGTCAAAGTGAATGACTGTGAGCGTTCTACTTGGCTTACTGCTAGAAGAGAAGGACACCCAGAAATTCTGGACGGTCAACAAATTCTCGATGATATCAACGATGAGTTAGCAAATCACGTTGATTCTTCGGATCTGTACATTGGGGTTACGAAACGTAGAAAAATCTCTATTGGGTCAGAGAAGCCAAGGTGGATCACGTCCTTGGAATACCATGGAGTTGTAGGAGGAGATCGATCAGTACCTTTATGTCAGTGGCGTCGGCATAAAAACTGGCGATTACTTCAACTTCTACCGTTCAGACCCCGAAACTGCATTAGCTTCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FIST FIST_C

Protein Analysis

431

Amino Acids

47.12

Weight (kDa)

8.61

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 121
AclWI GGATC 4 cut(s) 587, 600, 1104, 1166
AcsI RAATTY 4 cut(s) 434, 617, 1029, 1047
AcyI GRCGYC 1 cut(s) 1222
AfaI GTAC 2 cut(s) 1103, 1206
AfiI CCNNNNNNNGG 2 cut(s) 64, 955
AgsI TTSAA 5 cut(s) 399, 439, 553, 905, 1250
AjiI CACGTC 2 cut(s) 666, 1164
AjnI CCWGG 2 cut(s) 167, 544
AluBI AGCT 5 cut(s) 208, 278, 308, 349, 1284
AluI AGCT 5 cut(s) 208, 278, 308, 349, 1284
Alw26I GTCTC 1 cut(s) 891
AlwI GGATC 4 cut(s) 587, 600, 1104, 1166
Ama87I CYCGRG 1 cut(s) 597
AoxI GGCC 3 cut(s) 53, 290, 697
ApeKI GCWGC 2 cut(s) 26, 183
ApoI RAATTY 4 cut(s) 434, 617, 1029, 1047
ArsI GACNNNNNNTTYG 2 cut(s) 917, 949
Asp700I GAANNNNTTC 1 cut(s) 270
AspLEI GCGC 1 cut(s) 123
AspS9I GGNCC 2 cut(s) 54, 369
AsuC2I CCSGG 2 cut(s) 671, 949
AsuHPI GGTGA 3 cut(s) 262, 479, 779
AvaI CYCGRG 1 cut(s) 597
AvaII GGWCC 1 cut(s) 369
AxyI CCTNAGG 1 cut(s) 240
BamHI GGATCC 1 cut(s) 592
BbvI GCAGC 2 cut(s) 13, 195
BccI CCATC 3 cut(s) 464, 572, 689
BceAI ACGGC 4 cut(s) 68, 78, 305, 532
BcgI CGANNNNNNTGC 3 cut(s) 34, 578, 612
BciT130I CCWGG 2 cut(s) 169, 546
BcnI CCSGG 2 cut(s) 671, 949
BcoDI GTCTC 1 cut(s) 891
BfaI CTAG 3 cut(s) 275, 810, 1008
BfmI CTRYAG 1 cut(s) 181
BglI GCCNNNNNGGC 1 cut(s) 289
BisI GCNGC 7 cut(s) 24, 27, 30, 66, 69, 150, 184
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 7 cut(s) 25, 28, 31, 67, 70, 151, 185
Bme1390I CCNGG 4 cut(s) 169, 546, 671, 949
Bme18I GGWCC 1 cut(s) 369
BmeT110I CYCGRG 1 cut(s) 597
BmgBI CACGTC 2 cut(s) 666, 1164
BmgT120I GGNCC 2 cut(s) 54, 369
BmiI GGNNCC 5 cut(s) 56, 173, 370, 594, 946
BmrFI CCNGG 4 cut(s) 169, 546, 671, 949
BmsI GCATC 8 cut(s) 20, 152, 409, 496, 528, 550, 715, 835
Bpu1102I GCTNAGC 1 cut(s) 279
BpuMI CCSGG 2 cut(s) 671, 949
Bsa29I ATCGAT 1 cut(s) 1198
BsaHI GRCGYC 1 cut(s) 1222
BsaJI CCNNGG 5 cut(s) 669, 867, 1151, 1167, 1177
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 955
Bse1I ACTGG 2 cut(s) 372, 1241
Bse21I CCTNAGG 1 cut(s) 240
BseBI CCWGG 2 cut(s) 169, 546
BseCI ATCGAT 1 cut(s) 1198
BseDI CCNNGG 5 cut(s) 669, 867, 1151, 1167, 1177
BseGI GGATG 3 cut(s) 511, 519, 564
BseLI CCNNNNNNNGG 2 cut(s) 64, 955
BseMII CTCAG 2 cut(s) 270, 663
BseNI ACTGG 2 cut(s) 372, 1241
BseRI GAGGAG 1 cut(s) 1206
BseXI GCAGC 2 cut(s) 13, 195
Bsh1236I CGCG 1 cut(s) 121
BshFI GGCC 3 cut(s) 55, 292, 699
BshVI ATCGAT 1 cut(s) 1198
BsiHKCI CYCGRG 1 cut(s) 597
BsiSI CCGG 2 cut(s) 671, 949
BslFI GGGAC 1 cut(s) 652
BslI CCNNNNNNNGG 2 cut(s) 64, 955
BsmAI GTCTC 1 cut(s) 891
BsmFI GGGAC 1 cut(s) 652
BsnI GGCC 3 cut(s) 55, 292, 699
BsoBI CYCGRG 1 cut(s) 597
Bsp1407I TGTACA 1 cut(s) 1101
Bsp143I GATC 5 cut(s) 592, 1096, 1158, 1195, 1199
Bsp1720I GCTNAGC 1 cut(s) 279
Bsp19I CCATGG 1 cut(s) 1177
BspANI GGCC 3 cut(s) 55, 292, 699
BspCNI CTCAG 2 cut(s) 271, 662
BspDI ATCGAT 1 cut(s) 1198
BspFNI CGCG 1 cut(s) 121
BspLI GGNNCC 5 cut(s) 56, 173, 370, 594, 946
BspMAI CTGCAG 1 cut(s) 185
BspPI GGATC 4 cut(s) 587, 600, 1104, 1166
BsrGI TGTACA 1 cut(s) 1101
BsrI ACTGG 2 cut(s) 372, 1241
BssECI CCNNGG 5 cut(s) 669, 867, 1151, 1167, 1177
BssMI GATC 5 cut(s) 592, 1096, 1158, 1195, 1199
BssNI GRCGYC 1 cut(s) 1222
BssT1I CCWWGG 4 cut(s) 867, 1151, 1167, 1177
Bst2UI CCWGG 2 cut(s) 169, 546
Bst4CI ACNGT 6 cut(s) 532, 757, 940, 983, 1040, 1260
Bst6I CTCTTC 2 cut(s) 409, 1006
BstACI GRCGYC 1 cut(s) 1222
BstAPI GCANNNNNTGC 1 cut(s) 506
BstAUI TGTACA 1 cut(s) 1101
BstC8I GCNNGC 3 cut(s) 154, 210, 747
BstDEI CTNAG 3 cut(s) 240, 279, 649
BstDSI CCRYGG 1 cut(s) 1177
BstF5I GGATG 3 cut(s) 511, 519, 564
BstFNI CGCG 1 cut(s) 121
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 5 cut(s) 595, 1099, 1161, 1198, 1202
BstMAI GTCTC 1 cut(s) 891
BstMBI GATC 5 cut(s) 592, 1096, 1158, 1195, 1199
BstMWI GCNNNNNNNGC 5 cut(s) 29, 149, 289, 506, 746
BstNI CCWGG 2 cut(s) 169, 546
BstSCI CCNGG 4 cut(s) 167, 544, 669, 947
BstSFI CTRYAG 1 cut(s) 181
BstUI CGCG 1 cut(s) 121
BstV1I GCAGC 2 cut(s) 13, 195
BstX2I RGATCY 2 cut(s) 592, 1096
BstYI RGATCY 2 cut(s) 592, 1096
Bsu15I ATCGAT 1 cut(s) 1198
Bsu36I CCTNAGG 1 cut(s) 240
BsuRI GGCC 3 cut(s) 55, 292, 699
BsuTUI ATCGAT 1 cut(s) 1198
BtgI CCRYGG 1 cut(s) 1177
BtrI CACGTC 2 cut(s) 666, 1164
BtsCI GGATG 3 cut(s) 511, 519, 564
BtsIMutI CAGTG 2 cut(s) 379, 1223
Cac8I GCNNGC 3 cut(s) 154, 210, 747
CfoI GCGC 1 cut(s) 123
Cfr13I GGNCC 2 cut(s) 54, 369
ClaI ATCGAT 1 cut(s) 1198
CseI GACGC 1 cut(s) 1211
Csp6I GTAC 2 cut(s) 1102, 1205
CviAII CATG 6 cut(s) 431, 625, 709, 887, 1178, 1288
CviQI GTAC 2 cut(s) 1102, 1205
DdeI CTNAG 3 cut(s) 240, 279, 649
DpnI GATC 5 cut(s) 594, 1098, 1160, 1197, 1201
DpnII GATC 5 cut(s) 592, 1096, 1158, 1195, 1199
Eam1104I CTCTTC 2 cut(s) 409, 1006
EarI CTCTTC 2 cut(s) 409, 1006
EciI GGCGGA 1 cut(s) 205
Eco130I CCWWGG 4 cut(s) 867, 1151, 1167, 1177
Eco32I GATATC 1 cut(s) 1060
Eco47I GGWCC 1 cut(s) 369
Eco81I CCTNAGG 1 cut(s) 240
Eco88I CYCGRG 1 cut(s) 597
EcoRI GAATTC 1 cut(s) 434
EcoRII CCWGG 2 cut(s) 167, 544
EcoRV GATATC 1 cut(s) 1060
EcoT14I CCWWGG 4 cut(s) 867, 1151, 1167, 1177
ErhI CCWWGG 4 cut(s) 867, 1151, 1167, 1177
FaeI CATG 6 cut(s) 434, 628, 712, 890, 1181, 1291
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FaqI GGGAC 1 cut(s) 652
FatI CATG 6 cut(s) 430, 624, 708, 886, 1177, 1287
FauI CCCGC 2 cut(s) 136, 576
Fnu4HI GCNGC 7 cut(s) 24, 27, 30, 66, 69, 150, 184
FokI GGATG 3 cut(s) 506, 518, 551
Fsp4HI GCNGC 7 cut(s) 24, 27, 30, 66, 69, 150, 184
FspBI CTAG 3 cut(s) 275, 810, 1008
GlaI GCGC 1 cut(s) 122
GluI GCNGC 7 cut(s) 24, 27, 30, 66, 69, 150, 184
HaeIII GGCC 3 cut(s) 55, 292, 699
HapII CCGG 2 cut(s) 671, 949
HgaI GACGC 1 cut(s) 1211
HhaI GCGC 1 cut(s) 123
Hin1I GRCGYC 1 cut(s) 1222
Hin1II CATG 6 cut(s) 434, 628, 712, 890, 1181, 1291
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HincII GTYRAC 1 cut(s) 1043
HindII GTYRAC 1 cut(s) 1043
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 3 cut(s) 189, 806, 1088
HpaII CCGG 2 cut(s) 671, 949
HphI GGTGA 3 cut(s) 262, 479, 779
Hpy166II GTNNAC 2 cut(s) 936, 1043
Hpy188I TCNGA 4 cut(s) 658, 1096, 1144, 1265
Hpy188III TCNNGA 5 cut(s) 599, 704, 810, 1034, 1052
Hpy8I GTNNAC 2 cut(s) 936, 1043
Hpy99I CGWCG 4 cut(s) 19, 22, 98, 1227
HpyAV CCTTC 5 cut(s) 110, 148, 247, 710, 1010
HpyCH4III ACNGT 6 cut(s) 532, 757, 940, 983, 1040, 1260
HpyCH4IV ACGT 4 cut(s) 665, 1083, 1120, 1163
HpyCH4V TGCA 5 cut(s) 183, 663, 830, 917, 1278
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 149, 289, 506, 746
HpyF3I CTNAG 3 cut(s) 240, 279, 649
HpySE526I ACGT 4 cut(s) 665, 1083, 1120, 1163
Hsp92I GRCGYC 1 cut(s) 1222
Hsp92II CATG 6 cut(s) 434, 628, 712, 890, 1181, 1291
HspAI GCGC 1 cut(s) 121
Kzo9I GATC 5 cut(s) 592, 1096, 1158, 1195, 1199
Lsp1109I GCAGC 2 cut(s) 13, 195
LweI GCATC 8 cut(s) 20, 152, 409, 496, 528, 550, 715, 835
MaeI CTAG 3 cut(s) 275, 810, 1008
MaeII ACGT 4 cut(s) 665, 1083, 1120, 1163
MaeIII GTNAC 4 cut(s) 573, 704, 850, 1111
MalI GATC 5 cut(s) 594, 1098, 1160, 1197, 1201
MboI GATC 5 cut(s) 592, 1096, 1158, 1195, 1199
MboII GAAGA 4 cut(s) 426, 494, 1023, 1083
MflI RGATCY 2 cut(s) 592, 1096
MluCI AATT 9 cut(s) 362, 434, 474, 617, 678, 905, 912, 1029, 1047
MlyI GAGTC 1 cut(s) 815
MmeI TCCRAC 2 cut(s) 655, 711
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 2 cut(s) 447, 633
MspA1I CMGCKG 2 cut(s) 149, 571
MspI CCGG 2 cut(s) 671, 949
MspR9I CCNGG 4 cut(s) 169, 546, 671, 949
MvaI CCWGG 2 cut(s) 169, 546
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 5 cut(s) 29, 149, 289, 506, 746
NciI CCSGG 2 cut(s) 671, 949
NcoI CCATGG 1 cut(s) 1177
NdeII GATC 5 cut(s) 592, 1096, 1158, 1195, 1199
NlaIII CATG 6 cut(s) 434, 628, 712, 890, 1181, 1291
NlaIV GGNNCC 5 cut(s) 56, 173, 370, 594, 946
NmuCI GTSAC 1 cut(s) 704
PaeR7I CTCGAG 1 cut(s) 597
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 2 cut(s) 189, 1088
PfoI TCCNGGA 1 cut(s) 167
PkrI GCNGC 7 cut(s) 25, 28, 31, 67, 70, 151, 185
PleI GAGTC 1 cut(s) 814
PpsI GAGTC 1 cut(s) 814
Psp6I CCWGG 2 cut(s) 167, 544
PspGI CCWGG 2 cut(s) 167, 544
PspN4I GGNNCC 5 cut(s) 56, 173, 370, 594, 946
PspPI GGNCC 2 cut(s) 54, 369
PstI CTGCAG 1 cut(s) 185
PsuI RGATCY 2 cut(s) 592, 1096
RsaI GTAC 2 cut(s) 1103, 1206
RsaNI GTAC 2 cut(s) 1102, 1205
SaqAI TTAA 2 cut(s) 447, 633
SatI GCNGC 7 cut(s) 24, 27, 30, 66, 69, 150, 184
Sau3AI GATC 5 cut(s) 592, 1096, 1158, 1195, 1199
Sau96I GGNCC 2 cut(s) 54, 369
SchI GAGTC 1 cut(s) 815
ScrFI CCNGG 4 cut(s) 169, 546, 671, 949
SfaNI GCATC 8 cut(s) 20, 152, 409, 496, 528, 550, 715, 835
SfcI CTRYAG 1 cut(s) 181
Sfr274I CTCGAG 1 cut(s) 597
SinI GGWCC 1 cut(s) 369
SlaI CTCGAG 1 cut(s) 597
SmlI CTYRAG 1 cut(s) 597
SmoI CTYRAG 1 cut(s) 597
Sse9I AATT 9 cut(s) 362, 434, 474, 617, 678, 905, 912, 1029, 1047
SspI AATATT 2 cut(s) 313, 408
SspMI CTAG 3 cut(s) 275, 810, 1008
StyD4I CCNGG 4 cut(s) 167, 544, 669, 947
StyI CCWWGG 4 cut(s) 867, 1151, 1167, 1177
TaaI ACNGT 6 cut(s) 532, 757, 940, 983, 1040, 1260
TaiI ACGT 4 cut(s) 668, 1086, 1123, 1166
TaqI TCGA 5 cut(s) 264, 356, 598, 1053, 1198
TasI AATT 9 cut(s) 362, 434, 474, 617, 678, 905, 912, 1029, 1047
TatI WGTACW 1 cut(s) 1101
TauI GCSGC 5 cut(s) 26, 32, 68, 71, 152
TfiI GAWTC 2 cut(s) 189, 1088
Tru1I TTAA 2 cut(s) 447, 633
Tru9I TTAA 2 cut(s) 447, 633
TscAI CASTG 2 cut(s) 379, 1223
TseFI GTSAC 1 cut(s) 704
TseI GCWGC 2 cut(s) 26, 183
Tsp45I GTSAC 1 cut(s) 704
TspDTI ATGAA 6 cut(s) 373, 447, 495, 610, 725, 1276
TspRI CASTG 2 cut(s) 379, 1223
VpaK11BI GGWCC 1 cut(s) 369
XapI RAATTY 4 cut(s) 434, 617, 1029, 1047
XbaI TCTAGA 1 cut(s) 809
XhoI CTCGAG 1 cut(s) 597
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 3 cut(s) 275, 810, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.