MD06G1192500.v1.1
BHLH Family

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004351
Physical Location & Seq
Forward (+)
32795393 .. 32799247
3855 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1192500.v1.1.491

Sequence Viewer

Length: 2319 bp
ATGAATCGGGTTTTACTTCCTAAGCATGTTGCTGCTGTTGTTAAGTGTCAAAAGGATCCGTTTAAAGCGCTTGAAATGTTTAACTCGGTGAAAAAGGAAGATGGGTTTAAGCACACATTGCTAACTTACAAATGCATGGTTGAAAAACTCGGGTTTCATGGCGAGTTTGAAGCCATGGAGCGTGTGATTTCTGACATGAGGATGAATGTTGATAATAGCTTACTAGAAGGAGTGTACATTGGAGCCATGAGGAGTTATGGAAGGAAAGGAAAGGTTGAAGAAGCTGTTAATGTGTTTCAGCGGATGGAGTTTTATAATTGTGAGCCCTCGGTTCAATCTTATAATGCAATCATGAATGTGCTCGTTGAGTATGGATATTTCAATCAAGCGCATAAGGTGTATATGGGGATGAGAGATAATGGGATTGATCCAGATGTGTATACTTATACAATACGGATAAAGTCTTTCTGTAAGACTAGCAGGCCTCATGTTGCTTTGAGACTGCTTAATAACATGCCTACCCAGGGGTGTGAGTTCAATGCCGTTGCATATTGTACGGTCATAGGTGGATTTTATGAAGAGAATTACCGGATCGAGGCATATGAATTGTTTAAAGACATGCTCGGGCAAGGTGTATGTCCCAACGTTACCACATTTAACAAGCTTATTCATACATTATCCAAGAAGGGTCATGTCCAAGAAATCGAAAAACTTCTCAACAAGGTTCTGAAGAGGGGTGTGTCTCCTAATTTGTTTACATTAAATATTTTCATTCAAGGGCTTTGCAACAGCAGTTCACTAAATTTGGCTGTCAGAATGCTGGATGGTTTTATGATAGAAGGTTTAACTCCGGATGTTGTCACATATAATACATTGATCTTTGGCTTGTGTAAAAACTTTAAGGTTGAGGAAGCGGAGGGCTATATGCATAAAATGGTGAACAATGGATTTTGGCCTGATGCCTTTACCTACAACAGTATTATCGATGGATATTGCAAATTGGGTATGATACAAAAAGCAGATAAGATTCTCAGTGATGCCATTTTCAAGGGATTCATACCCGATGACTTCACATATTGCTCTTTGATTAAGGGTTTATGCCAGGATGGCCAGATTGACCGTGCCCTAGCTTTGTTCAATGAGGTATTAGGAAAAGGATTCAAGCCTAATATTGTTTTCTATAACACGTTGGTAAAAGGGTTGTCTCAGAATGGGCTTTTCTTGGAAGCTTTGCAACTGATGAATGAGATGTCAAGAAACAGTTGCAGTCCCGATATCTGGACTTACAACTTGGTTATAAACGGGTTGTGCAAGATGGGTTATGTATCTGATGCCAGTAAACTAATGAATGATGCTATTGCCAGAGGATGCCTTCCCGACATATTTACTTTCAATACGTTAATAGATGGTTACTGTAAACAGTTGAATCTGAATAGTGCTCTTGAGGTCATTAGTAGCATGTGGAGTCATGGTGTTACTCCTGATGTGATCACGTACAATACTGTGTTAAATGGCCTTTGCAAGGCTGCCAAATACGAGGATGTGATCCGTACTTTTCAAGCAATGATGGAGAAGGGGTGTGTACCTAACATTATTACATATAACATACTTGTAGAAAGCCTTTGCAAAGCCAGGAAAGTGACCGAGGCCTTGGACATGCTACGGGAAATAAGAGACAAGGGCTTGACTCCAGATATTGTTTGTTTTGGCACATTGATGAATGGTTTGTGTGGCAATGGGGATTTGGACGGAGCATATGACCTATTTACAAGAATGAAACATGAATATAATGTATCTCATACAACAGCGACATACAATATCATGATCAATGCTTTCTGTGAAAATCTTAACAGTAGCATAGCACAAAAACTATTTTGTGAGATGGGTGACAAAGGATGTGCCCCCGACTGTTTCACTTACCGTGTCATGATTGATGGTTTCTGTAAAGCAGGAAATGATGATCATGGGTACAATTTTCTCCTGGAGAAGATCGAGAAGGGGTTCATTCCATCACTGGTGACATTCGGAAGAGTGCTAAATTGTCTTTGTGTGAAGCATAGAGTTCAGGAGGCTGTTGGTATCGTCTACCTTATGGTGCAGAAAGGCATTGTTCCTGATGTTGTTAGTACAATCTTCGAGTCTGATAAGAAGGAAATAGCTGCACCCAAGATTGTTGTAGAAGATTTGTTGAAGAAGGGTCATATAACTTATTATGCCTATGAACTTCTATATGATGGAATTAGAGATAAAAAGGTAGTTAAGAAAACACAAAAGAGCTTCAAACGAGGTGTTTCCATGACTTCAAAACGAAGTCCTTGA

Protein Analysis

773

Amino Acids

87.13

Weight (kDa)

8.08

Isoelectric Point (pI)

24.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 83 - 120 4.6e-07 PPR repeat family
PPR_2 PF13041 109 - 158 9.6e-14 PPR repeat family
PPR_1 PF12854 140 - 172 8.2e-06 PPR repeat
PPR_2 PF13041 180 - 228 2.8e-08 PPR repeat family
PPR_3 PF13812 203 - 259 1.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 214 - 263 9.2e-14 PPR repeat family
PPR_1 PF12854 281 - 313 7.8e-11 PPR repeat
PPR_2 PF13041 284 - 333 1.1e-16 PPR repeat family
PPR PF01535 287 - 317 3.8e-07 PPR repeat
PPR_1 PF12854 316 - 342 1.6e-07 PPR repeat
PPR_2 PF13041 319 - 368 4.9e-15 PPR repeat family
PPR PF01535 322 - 351 4.8e-06 PPR repeat
PPR_3 PF13812 342 - 399 7e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 350 - 381 8.6e-10 PPR repeat
PPR PF01535 357 - 387 2.9e-06 PPR repeat
PPR_3 PF13812 378 - 434 3.6e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 385 - 418 5.2e-08 PPR repeat
PPR_2 PF13041 389 - 438 8.1e-17 PPR repeat family
PPR PF01535 392 - 422 4.5e-06 PPR repeat
PPR_1 PF12854 421 - 450 1.7e-09 PPR repeat
PPR_2 PF13041 424 - 473 9.5e-16 PPR repeat family
PPR_3 PF13812 448 - 504 4.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 455 - 487 1.4e-10 PPR repeat
PPR_2 PF13041 459 - 508 4.4e-17 PPR repeat family
PPR PF01535 462 - 492 9.3e-06 PPR repeat
PPR_3 PF13812 489 - 539 5.4e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 491 - 514 4e-07 PPR repeat
PPR_2 PF13041 494 - 543 7.3e-17 PPR repeat family
PPR_1 PF12854 525 - 557 5.9e-11 PPR repeat
PPR_2 PF13041 529 - 577 3.3e-13 PPR repeat family
PPR PF01535 532 - 562 9.8e-06 PPR repeat
PPR_3 PF13812 553 - 614 5.4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 560 - 593 1.8e-09 PPR repeat
PPR_2 PF13041 564 - 613 7.3e-13 PPR repeat family
PPR_2 PF13041 601 - 649 3.7e-16 PPR repeat family
PPR PF01535 604 - 633 7.6e-06 PPR repeat
PPR_1 PF12854 631 - 652 2.7e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 315, 342, 1298
AccI GTMKAC 2 cut(s) 440, 2085
AccIII TCCGGA 1 cut(s) 850
AciI CCGC 2 cut(s) 301, 914
AclI AACGTT 1 cut(s) 645
AclWI GGATC 5 cut(s) 50, 63, 422, 599, 1540
AcoI YGGCCR 1 cut(s) 1108
AcsI RAATTY 1 cut(s) 802
AcuI CTGAAG 1 cut(s) 749
AfaI GTAC 7 cut(s) 236, 556, 1496, 1552, 1584, 1970, 2128
AfeI AGCGCT 1 cut(s) 69
AfiI CCNNNNNNNGG 4 cut(s) 524, 595, 1278, 1522
AflIII ACRYGT 1 cut(s) 1185
AjnI CCWGG 4 cut(s) 522, 1101, 1631, 1980
AluBI AGCT 7 cut(s) 219, 284, 664, 1130, 1229, 2159, 2277
AluI AGCT 7 cut(s) 219, 284, 664, 1130, 1229, 2159, 2277
Alw21I GWGCWC 2 cut(s) 363, 1441
Alw26I GTCTC 4 cut(s) 493, 747, 1209, 1668
AlwI GGATC 5 cut(s) 50, 63, 422, 599, 1540
Ama87I CYCGRG 2 cut(s) 149, 623
Aor13HI TCCGGA 1 cut(s) 850
Aor51HI AGCGCT 1 cut(s) 69
AoxI GGCC 5 cut(s) 482, 953, 1108, 1513, 1647
ApeKI GCWGC 3 cut(s) 32, 1526, 2159
ApoI RAATTY 1 cut(s) 802
Asp700I GAANNNNTTC 2 cut(s) 711, 2000
AspLEI GCGC 2 cut(s) 70, 391
AsuHPI GGTGA 4 cut(s) 100, 949, 1898, 2029
AvaI CYCGRG 2 cut(s) 149, 623
BaeGI GKGCMC 2 cut(s) 1126, 1903
BalI TGGCCA 1 cut(s) 1110
BamHI GGATCC 1 cut(s) 55
BanII GRGCYC 1 cut(s) 327
Bbv12I GWGCWC 2 cut(s) 363, 1441
BbvI GCAGC 3 cut(s) 19, 1513, 2146
BceAI ACGGC 1 cut(s) 527
BciT130I CCWGG 4 cut(s) 524, 1103, 1633, 1982
BclI TGATCA 3 cut(s) 1488, 1824, 1960
BcoDI GTCTC 4 cut(s) 493, 747, 1209, 1668
BfaI CTAG 3 cut(s) 224, 477, 1127
BfoI RGCGCY 1 cut(s) 71
BglI GCCNNNNNGGC 1 cut(s) 1107
BisI GCNGC 3 cut(s) 33, 1527, 2160
BlsI GCNGC 3 cut(s) 34, 1528, 2161
Bme1390I CCNGG 4 cut(s) 524, 1103, 1633, 1982
BmeT110I CYCGRG 2 cut(s) 149, 623
BmiI GGNNCC 2 cut(s) 57, 244
BmrFI CCNGG 4 cut(s) 524, 1103, 1633, 1982
BmsI GCATC 5 cut(s) 949, 1027, 1321, 1342, 1358
BpmI CTGGAG 2 cut(s) 1674, 2003
Bpu10I CCTNAGC 1 cut(s) 21
BpuEI CTTGAG 1 cut(s) 1463
Bsa29I ATCGAT 1 cut(s) 984
BsaAI YACGTR 1 cut(s) 1494
BsaJI CCNNGG 6 cut(s) 174, 327, 522, 523, 1644, 1650
BsaWI WCCGGW 2 cut(s) 588, 850
BsaXI ACNNNNNCTCC 4 cut(s) 1456, 1486, 1562, 1592
Bsc4I CCNNNNNNNGG 4 cut(s) 524, 595, 1278, 1522
Bse1I ACTGG 2 cut(s) 1335, 2019
Bse3DI GCAATG 3 cut(s) 116, 1569, 1741
BseAI TCCGGA 1 cut(s) 850
BseBI CCWGG 4 cut(s) 524, 1103, 1633, 1982
BseCI ATCGAT 1 cut(s) 984
BseDI CCNNGG 6 cut(s) 174, 327, 522, 523, 1644, 1650
BseGI GGATG 9 cut(s) 207, 309, 414, 829, 859, 1111, 1373, 1546, 1901
BseLI CCNNNNNNNGG 4 cut(s) 524, 595, 1278, 1522
BseMI GCAATG 3 cut(s) 116, 1569, 1741
BseMII CTCAG 2 cut(s) 1045, 1220
BseNI ACTGG 2 cut(s) 1335, 2019
BseRI GAGGAG 1 cut(s) 265
BseSI GKGCMC 2 cut(s) 1126, 1903
BseXI GCAGC 3 cut(s) 19, 1513, 2146
BsgI GTGCAG 2 cut(s) 2117, 2145
BshFI GGCC 5 cut(s) 484, 955, 1110, 1515, 1649
BshVI ATCGAT 1 cut(s) 984
BsiHKAI GWGCWC 2 cut(s) 363, 1441
BsiHKCI CYCGRG 2 cut(s) 149, 623
BsiSI CCGG 2 cut(s) 589, 851
BslFI GGGAC 2 cut(s) 624, 1254
BslI CCNNNNNNNGG 4 cut(s) 524, 595, 1278, 1522
BsmAI GTCTC 4 cut(s) 493, 747, 1209, 1668
BsmFI GGGAC 2 cut(s) 624, 1254
BsmI GAATGC 1 cut(s) 822
BsnI GGCC 5 cut(s) 484, 955, 1110, 1515, 1649
BsoBI CYCGRG 2 cut(s) 149, 623
Bsp1286I GDGCHC 5 cut(s) 327, 363, 1126, 1441, 1903
Bsp13I TCCGGA 1 cut(s) 850
Bsp1407I TGTACA 1 cut(s) 234
Bsp143I GATC 9 cut(s) 55, 427, 591, 876, 1488, 1545, 1824, 1960, 1989
Bsp19I CCATGG 1 cut(s) 174
BspACI CCGC 2 cut(s) 301, 914
BspANI GGCC 5 cut(s) 484, 955, 1110, 1515, 1649
BspCNI CTCAG 2 cut(s) 1044, 1219
BspDI ATCGAT 1 cut(s) 984
BspEI TCCGGA 1 cut(s) 850
BspHI TCATGA 3 cut(s) 351, 1821, 1926
BspLI GGNNCC 2 cut(s) 57, 244
BspPI GGATC 5 cut(s) 50, 63, 422, 599, 1540
BsrDI GCAATG 3 cut(s) 116, 1569, 1741
BsrGI TGTACA 1 cut(s) 234
BsrI ACTGG 2 cut(s) 1335, 2019
BssECI CCNNGG 6 cut(s) 174, 327, 522, 523, 1644, 1650
BssMI GATC 9 cut(s) 55, 427, 591, 876, 1488, 1545, 1824, 1960, 1989
BssNAI GTATAC 1 cut(s) 441
BssT1I CCWWGG 2 cut(s) 174, 1650
Bst1107I GTATAC 1 cut(s) 441
Bst2UI CCWGG 4 cut(s) 524, 1103, 1633, 1982
Bst6I CTCTTC 3 cut(s) 573, 725, 2023
BstAPI GCANNNNNTGC 1 cut(s) 118
BstAUI TGTACA 1 cut(s) 234
BstBAI YACGTR 1 cut(s) 1494
BstC8I GCNNGC 1 cut(s) 482
BstDEI CTNAG 3 cut(s) 21, 1031, 1206
BstDSI CCRYGG 1 cut(s) 174
BstENI CCTNNNNNAGG 1 cut(s) 1520
BstF5I GGATG 9 cut(s) 207, 309, 414, 829, 859, 1111, 1373, 1546, 1901
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 2 cut(s) 70, 391
BstKTI GATC 9 cut(s) 58, 430, 594, 879, 1491, 1548, 1827, 1963, 1992
BstMAI GTCTC 4 cut(s) 493, 747, 1209, 1668
BstMBI GATC 9 cut(s) 55, 427, 591, 876, 1488, 1545, 1824, 1960, 1989
BstMWI GCNNNNNNNGC 2 cut(s) 118, 1107
BstNI CCWGG 4 cut(s) 524, 1103, 1633, 1982
BstNSI RCATGY 5 cut(s) 29, 517, 622, 1462, 1660
BstSCI CCNGG 4 cut(s) 522, 1101, 1631, 1980
BstSLI GKGCMC 2 cut(s) 1126, 1903
BstV1I GCAGC 3 cut(s) 19, 1513, 2146
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
BstZ17I GTATAC 1 cut(s) 441
Bsu15I ATCGAT 1 cut(s) 984
BsuRI GGCC 5 cut(s) 484, 955, 1110, 1515, 1649
BsuTUI ATCGAT 1 cut(s) 984
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 9 cut(s) 207, 309, 414, 829, 859, 1111, 1373, 1546, 1901
BtsIMutI CAGTG 2 cut(s) 1039, 2012
Cac8I GCNNGC 1 cut(s) 482
CciI TCATGA 3 cut(s) 351, 1821, 1926
CfoI GCGC 2 cut(s) 70, 391
ClaI ATCGAT 1 cut(s) 984
Csp6I GTAC 7 cut(s) 235, 555, 1495, 1551, 1583, 1969, 2127
CviQI GTAC 7 cut(s) 235, 555, 1495, 1551, 1583, 1969, 2127
DdeI CTNAG 3 cut(s) 21, 1031, 1206
DpnI GATC 9 cut(s) 57, 429, 593, 878, 1490, 1547, 1826, 1962, 1991
DpnII GATC 9 cut(s) 55, 427, 591, 876, 1488, 1545, 1824, 1960, 1989
DraI TTTAAA 2 cut(s) 64, 613
EaeI YGGCCR 1 cut(s) 1108
Eam1104I CTCTTC 3 cut(s) 573, 725, 2023
EarI CTCTTC 3 cut(s) 573, 725, 2023
Eco130I CCWWGG 2 cut(s) 174, 1650
Eco147I AGGCCT 2 cut(s) 484, 1649
Eco24I GRGCYC 1 cut(s) 327
Eco32I GATATC 1 cut(s) 1276
Eco47III AGCGCT 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 749
Eco88I CYCGRG 2 cut(s) 149, 623
EcoNI CCTNNNNNAGG 1 cut(s) 1520
EcoRII CCWGG 4 cut(s) 522, 1101, 1631, 1980
EcoRV GATATC 1 cut(s) 1276
EcoT14I CCWWGG 2 cut(s) 174, 1650
EcoT22I ATGCAT 2 cut(s) 137, 930
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 2 cut(s) 174, 1650
FaqI GGGAC 2 cut(s) 624, 1254
FauNDI CATATG 2 cut(s) 601, 1756
FbaI TGATCA 3 cut(s) 1488, 1824, 1960
FblI GTMKAC 2 cut(s) 440, 2085
Fnu4HI GCNGC 3 cut(s) 33, 1527, 2160
FokI GGATG 9 cut(s) 214, 316, 421, 836, 866, 1118, 1380, 1553, 1908
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 3 cut(s) 33, 1527, 2160
FspBI CTAG 3 cut(s) 224, 477, 1127
GlaI GCGC 2 cut(s) 69, 390
GluI GCNGC 3 cut(s) 33, 1527, 2160
GsuI CTGGAG 2 cut(s) 1674, 2003
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 5 cut(s) 484, 955, 1110, 1515, 1649
HapII CCGG 2 cut(s) 589, 851
HhaI GCGC 2 cut(s) 70, 391
Hin6I GCGC 2 cut(s) 68, 389
HinP1I GCGC 2 cut(s) 68, 389
HindIII AAGCTT 2 cut(s) 662, 1227
HinfI GANTC 8 cut(s) 4, 1027, 1053, 1158, 1426, 1465, 1687, 2138
HpaII CCGG 2 cut(s) 589, 851
HphI GGTGA 4 cut(s) 100, 949, 1898, 2029
Hpy166II GTNNAC 9 cut(s) 235, 441, 756, 797, 940, 1340, 1418, 1583, 2086
Hpy188I TCNGA 8 cut(s) 193, 729, 815, 1209, 1330, 1431, 2027, 2143
Hpy8I GTNNAC 9 cut(s) 235, 441, 756, 797, 940, 1340, 1418, 1583, 2086
HpyAV CCTTC 9 cut(s) 221, 255, 679, 833, 1382, 1567, 1990, 2143, 2188
HpyCH4IV ACGT 4 cut(s) 645, 1187, 1397, 1493
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 1107
HpyF3I CTNAG 3 cut(s) 21, 1031, 1206
HpySE526I ACGT 4 cut(s) 645, 1187, 1397, 1493
HspAI GCGC 2 cut(s) 68, 389
Kpn2I TCCGGA 1 cut(s) 850
Ksp22I TGATCA 3 cut(s) 1488, 1824, 1960
Kzo9I GATC 9 cut(s) 55, 427, 591, 876, 1488, 1545, 1824, 1960, 1989
LmnI GCTCC 3 cut(s) 178, 242, 1751
Lsp1109I GCAGC 3 cut(s) 19, 1513, 2146
LweI GCATC 5 cut(s) 949, 1027, 1321, 1342, 1358
MaeI CTAG 3 cut(s) 224, 477, 1127
MaeII ACGT 4 cut(s) 645, 1187, 1397, 1493
MaeIII GTNAC 7 cut(s) 646, 859, 1409, 1474, 1639, 1886, 2017
MalI GATC 9 cut(s) 57, 429, 593, 878, 1490, 1547, 1826, 1962, 1991
MboI GATC 9 cut(s) 55, 427, 591, 876, 1488, 1545, 1824, 1960, 1989
MboII GAAGA 9 cut(s) 110, 290, 590, 742, 1999, 2040, 2125, 2192, 2203
MflI RGATCY 1 cut(s) 55
MhlI GDGCHC 5 cut(s) 327, 363, 1126, 1441, 1903
MlsI TGGCCA 1 cut(s) 1110
MluCI AATT 9 cut(s) 316, 583, 605, 748, 802, 998, 1972, 2038, 2238
MluNI TGGCCA 1 cut(s) 1110
MlyI GAGTC 3 cut(s) 1474, 1681, 2147
Mox20I TGGCCA 1 cut(s) 1110
Mph1103I ATGCAT 2 cut(s) 137, 930
MroI TCCGGA 1 cut(s) 850
MroXI GAANNNNTTC 2 cut(s) 711, 2000
MscI TGGCCA 1 cut(s) 1110
MslI CAYNNNNRTG 3 cut(s) 200, 356, 1715
Msp20I TGGCCA 1 cut(s) 1110
MspA1I CMGCKG 1 cut(s) 301
MspI CCGG 2 cut(s) 589, 851
MspR9I CCNGG 4 cut(s) 524, 1103, 1633, 1982
Mva1269I GAATGC 1 cut(s) 822
MvaI CCWGG 4 cut(s) 524, 1103, 1633, 1982
MwoI GCNNNNNNNGC 2 cut(s) 118, 1107
NcoI CCATGG 1 cut(s) 174
NdeI CATATG 2 cut(s) 601, 1756
NdeII GATC 9 cut(s) 55, 427, 591, 876, 1488, 1545, 1824, 1960, 1989
NlaIV GGNNCC 2 cut(s) 57, 244
NmuCI GTSAC 4 cut(s) 859, 1639, 1886, 2017
NsiI ATGCAT 2 cut(s) 137, 930
NspI RCATGY 5 cut(s) 29, 517, 622, 1462, 1660
PagI TCATGA 3 cut(s) 351, 1821, 1926
PasI CCCWGGG 1 cut(s) 523
PceI AGGCCT 2 cut(s) 484, 1649
PctI GAATGC 1 cut(s) 822
PdmI GAANNNNTTC 2 cut(s) 711, 2000
PfeI GAWTC 5 cut(s) 4, 1027, 1053, 1158, 1426
PfoI TCCNGGA 1 cut(s) 1980
PkrI GCNGC 3 cut(s) 34, 1528, 2161
PleI GAGTC 3 cut(s) 1473, 1681, 2146
PpsI GAGTC 3 cut(s) 1473, 1681, 2146
Ppu21I YACGTR 1 cut(s) 1494
PsiI TTATAA 3 cut(s) 315, 342, 1298
Psp1406I AACGTT 1 cut(s) 645
Psp6I CCWGG 4 cut(s) 522, 1101, 1631, 1980
PspGI CCWGG 4 cut(s) 522, 1101, 1631, 1980
PspN4I GGNNCC 2 cut(s) 57, 244
PsuI RGATCY 1 cut(s) 55
RsaI GTAC 7 cut(s) 236, 556, 1496, 1552, 1584, 1970, 2128
RsaNI GTAC 7 cut(s) 235, 555, 1495, 1551, 1583, 1969, 2127
RseI CAYNNNNRTG 3 cut(s) 200, 356, 1715
SatI GCNGC 3 cut(s) 33, 1527, 2160
Sau3AI GATC 9 cut(s) 55, 427, 591, 876, 1488, 1545, 1824, 1960, 1989
SchI GAGTC 3 cut(s) 1474, 1681, 2147
ScrFI CCNGG 4 cut(s) 524, 1103, 1633, 1982
SduI GDGCHC 5 cut(s) 327, 363, 1126, 1441, 1903
SfaNI GCATC 5 cut(s) 949, 1027, 1321, 1342, 1358
SmiMI CAYNNNNRTG 3 cut(s) 200, 356, 1715
SmlI CTYRAG 1 cut(s) 1442
SmoI CTYRAG 1 cut(s) 1442
Sse9I AATT 9 cut(s) 316, 583, 605, 748, 802, 998, 1972, 2038, 2238
SseBI AGGCCT 2 cut(s) 484, 1649
SsiI CCGC 2 cut(s) 301, 914
SspI AATATT 2 cut(s) 766, 1171
SspMI CTAG 3 cut(s) 224, 477, 1127
StuI AGGCCT 2 cut(s) 484, 1649
StyD4I CCNGG 4 cut(s) 522, 1101, 1631, 1980
StyI CCWWGG 2 cut(s) 174, 1650
TaiI ACGT 4 cut(s) 648, 1190, 1400, 1496
TaqI TCGA 5 cut(s) 594, 705, 984, 1992, 2136
TaqII GACCGA 1 cut(s) 1658
TasI AATT 9 cut(s) 316, 583, 605, 748, 802, 998, 1972, 2038, 2238
TatI WGTACW 2 cut(s) 234, 2126
TfiI GAWTC 5 cut(s) 4, 1027, 1053, 1158, 1426
TscAI CASTG 2 cut(s) 1039, 2019
TseFI GTSAC 4 cut(s) 859, 1639, 1886, 2017
TseI GCWGC 3 cut(s) 32, 1526, 2159
Tsp45I GTSAC 4 cut(s) 859, 1639, 1886, 2017
TspGWI ACGGA 4 cut(s) 48, 469, 1538, 1764
TspRI CASTG 2 cut(s) 1039, 2019
XagI CCTNNNNNAGG 1 cut(s) 1520
XapI RAATTY 1 cut(s) 802
XceI RCATGY 5 cut(s) 29, 517, 622, 1462, 1660
XmiI GTMKAC 2 cut(s) 440, 2085
XmnI GAANNNNTTC 2 cut(s) 711, 2000
XspI CTAG 3 cut(s) 224, 477, 1127
Zsp2I ATGCAT 2 cut(s) 137, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.