MD06G1192900.v1.1
MYB Family

Myb-related protein 306-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009059
Physical Location & Seq
Forward (+)
32831447 .. 32834228
2782 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1192900.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGGGAAGGCCACCTTGCTGTGACAAAGTTGGTGTGAAGAAGGGCCCGTGGACTCCAGAGGAAGACATCATCTTGGTCTCTTACATTCAAGAACATGGTCCTGGGAATTGGAGATCAGTTCCAACTAACACTGGTTTGCTGAGATGCAGTAAGAGTTGCAGACTTAGATGGACTAATTATCTCCGCCCGGGTATCAAACGCGGTAACTTCACTGATCATGAGGAGAAGATGATAATCCACCTCCAAGCTCTTCTGGGCAATAGATGGGCAGCCATAGCTTCATATCTTCCTCAGAGAACAGACAACGACATAAAAAATTACTGGAACACCCACTTGAAAAAGAAGCTGAAAAAGCTTCAGACAGGACTTGATGGTCATGACCATCACAACCACCAGGATGGTAATTCACATGATCAGCCAATCTCCAAGGGTCAATGGGAGAGAAGGCTTCAGACTGATATCCACATGGCCAAGCAAGCTCTTTGTGAGGCTCTCTCCATTGACAAACCAAATGTTATTAGTACCACTACTGTTTCTCATGACCTTCACTTGCAAGACTTGAAGCCCTCCATTAATATAGGTAATTACAATATTAATGATAACCCTAGCACTAGCACAAGGCCACATCATACCTCCACGTATGCATCCAATACCGAAAACATTGCGAAATTGCTCGAAAACTGGATGAAAAATTCGCCAAAGGTTTCCGCTCATCAAACAAACTCGGATCAGACCAATGCTGATCAGACCAATTCCTTCAACAACAACAACAACAACGACAACGACAACAAAGGTGGCGCTACTGCAACTACTAGAGGTCGTTCAATGAGCACTTCTAGTGAAGGGGCGCACAGCGCAACCACCACGCCGGATCAGGCTTTCGATTCGTTGTTCAGCTTCAACTCATCCCCATCGGATGTGTCTCAGTCCATGTCAGTGGATGAGAACAATGCAAATTTCATAGCAGATCAGGCAAGCTGTCTGTTTCAGGATGAGAGCAAGCCCAATTTGGAGGGACAAGTCCCTCTCTCATTGCTGGAGAAGTGGCTCCTTGACGATGCTGCACCTCATGCCCACGAAGACCTAATCAACATATCATTGGAGAATGGTGCAGGCTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009751 GO:0009753 GO:0009787 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010038 GO:0010115 GO:0010166 GO:0010468 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0014070 GO:0019216 GO:0019219 GO:0019222 GO:0019747 GO:0023051 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0033993 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043455 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046677 GO:0046686 GO:0046890 GO:0048518 GO:0048522 GO:0048580 GO:0048583 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0060255 GO:0062012 GO:0065007 GO:0071704 GO:0080050 GO:0080090 GO:0080167 GO:0097159 GO:0097305 GO:0140110 GO:1901363 GO:1901419 GO:1901568 GO:1901570 GO:1901576 GO:1901700 GO:1902680 GO:1902930 GO:1903506 GO:1903508 GO:1904276 GO:1904278 GO:1905957 GO:2000026 GO:2000033 GO:2000034 GO:2000112 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

375

Amino Acids

41.67

Weight (kDa)

6.21

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 2.1e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 2.7e-10 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 1.8e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 70 - 118 3.1e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 372
AccBSI CCGCTC 1 cut(s) 710
AccII CGCG 1 cut(s) 201
AciI CCGC 3 cut(s) 184, 201, 708
AclWI GGATC 2 cut(s) 735, 879
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 2 cut(s) 691, 955
AcuI CTGAAG 2 cut(s) 341, 434
AfaI GTAC 1 cut(s) 523
AgsI TTSAA 6 cut(s) 89, 337, 562, 760, 825, 901
AjnI CCWGG 2 cut(s) 100, 393
AluBI AGCT 7 cut(s) 248, 278, 346, 355, 479, 897, 978
AluI AGCT 7 cut(s) 248, 278, 346, 355, 479, 897, 978
Alw21I GWGCWC 1 cut(s) 833
Alw26I GTCTC 2 cut(s) 82, 927
AlwI GGATC 2 cut(s) 735, 879
Ama87I CYCGRG 1 cut(s) 187
AoxI GGCC 4 cut(s) 8, 43, 468, 620
ApaI GGGCCC 1 cut(s) 47
ApeKI GCWGC 2 cut(s) 269, 1061
ApoI RAATTY 2 cut(s) 691, 955
AseI ATTAAT 2 cut(s) 573, 594
AspLEI GCGC 3 cut(s) 800, 850, 857
AspS9I GGNCC 3 cut(s) 43, 44, 98
AsuC2I CCSGG 2 cut(s) 188, 189
AvaI CYCGRG 1 cut(s) 187
AvaII GGWCC 1 cut(s) 98
BaeGI GKGCMC 1 cut(s) 47
BalI TGGCCA 1 cut(s) 470
BanII GRGCYC 1 cut(s) 47
BbsI GAAGAC 2 cut(s) 69, 1086
Bbv12I GWGCWC 1 cut(s) 833
BbvI GCAGC 2 cut(s) 281, 1048
BccI CCATC 6 cut(s) 162, 258, 365, 390, 392, 919
BcgI CGANNNNNNTGC 2 cut(s) 644, 678
BciT130I CCWGG 2 cut(s) 102, 395
BclI TGATCA 3 cut(s) 214, 412, 742
BcnI CCSGG 2 cut(s) 188, 189
BcoDI GTCTC 2 cut(s) 82, 927
BfaI CTAG 4 cut(s) 606, 612, 813, 837
BfoI RGCGCY 1 cut(s) 801
BisI GCNGC 2 cut(s) 270, 1062
BlsI GCNGC 2 cut(s) 271, 1063
Bme1390I CCNGG 4 cut(s) 102, 188, 189, 395
Bme18I GGWCC 1 cut(s) 98
BmeT110I CYCGRG 1 cut(s) 187
BmgT120I GGNCC 3 cut(s) 43, 44, 98
BmiI GGNNCC 2 cut(s) 45, 1049
BmrFI CCNGG 4 cut(s) 102, 188, 189, 395
BmsI GCATC 3 cut(s) 134, 653, 1048
BpiI GAAGAC 2 cut(s) 69, 1086
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 2 cut(s) 39, 1058
BpuMI CCSGG 2 cut(s) 188, 189
BsaAI YACGTR 1 cut(s) 639
BsaBI GATNNNNATC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 4 cut(s) 47, 101, 187, 426
Bse1I ACTGG 3 cut(s) 136, 326, 686
Bse3DI GCAATG 2 cut(s) 660, 1031
Bse8I GATNNNNATC 1 cut(s) 233
BseBI CCWGG 2 cut(s) 102, 395
BseDI CCNNGG 4 cut(s) 47, 101, 187, 426
BseGI GGATG 7 cut(s) 403, 644, 690, 905, 922, 946, 997
BseJI GATNNNNATC 1 cut(s) 233
BseMI GCAATG 2 cut(s) 660, 1031
BseMII CTCAG 3 cut(s) 131, 305, 938
BseNI ACTGG 3 cut(s) 136, 326, 686
BseRI GAGGAG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 47
BseXI GCAGC 2 cut(s) 281, 1048
BsgI GTGCAG 1 cut(s) 1047
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 4 cut(s) 10, 45, 470, 622
BsiHKAI GWGCWC 1 cut(s) 833
BsiHKCI CYCGRG 1 cut(s) 187
BsiSI CCGG 2 cut(s) 188, 869
BslFI GGGAC 2 cut(s) 1007, 1029
BsmAI GTCTC 2 cut(s) 82, 927
BsmFI GGGAC 2 cut(s) 1007, 1029
BsnI GGCC 4 cut(s) 10, 45, 470, 622
Bso31I GGTCTC 1 cut(s) 82
BsoBI CYCGRG 1 cut(s) 187
Bsp120I GGGCCC 1 cut(s) 43
Bsp1286I GDGCHC 2 cut(s) 47, 833
Bsp143I GATC 7 cut(s) 113, 214, 412, 727, 742, 871, 967
BspACI CCGC 3 cut(s) 184, 201, 708
BspANI GGCC 4 cut(s) 10, 45, 470, 622
BspCNI CTCAG 3 cut(s) 132, 304, 937
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 3 cut(s) 217, 376, 538
BspLI GGNNCC 2 cut(s) 45, 1049
BspPI GGATC 2 cut(s) 735, 879
BspQI GCTCTTC 1 cut(s) 255
BspTNI GGTCTC 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 710
BsrDI GCAATG 2 cut(s) 660, 1031
BsrI ACTGG 3 cut(s) 136, 326, 686
BssECI CCNNGG 4 cut(s) 47, 101, 187, 426
BssMI GATC 7 cut(s) 113, 214, 412, 727, 742, 871, 967
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 2 cut(s) 102, 395
Bst4CI ACNGT 1 cut(s) 532
Bst6I CTCTTC 1 cut(s) 255
BstAPI GCANNNNNTGC 1 cut(s) 1070
BstBAI YACGTR 1 cut(s) 639
BstC8I GCNNGC 4 cut(s) 477, 976, 1001, 1114
BstDEI CTNAG 4 cut(s) 140, 164, 291, 924
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 7 cut(s) 403, 644, 690, 905, 922, 946, 997
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 801
BstHHI GCGC 3 cut(s) 800, 850, 857
BstKTI GATC 7 cut(s) 116, 217, 415, 730, 745, 874, 970
BstMAI GTCTC 2 cut(s) 82, 927
BstMBI GATC 7 cut(s) 113, 214, 412, 727, 742, 871, 967
BstMWI GCNNNNNNNGC 6 cut(s) 275, 352, 476, 854, 971, 1070
BstNI CCWGG 2 cut(s) 102, 395
BstSCI CCNGG 4 cut(s) 100, 186, 187, 393
BstSLI GKGCMC 1 cut(s) 47
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 2 cut(s) 281, 1048
BstV2I GAAGAC 2 cut(s) 69, 1086
BstXI CCANNNNNNTGG 2 cut(s) 398, 937
BsuRI GGCC 4 cut(s) 10, 45, 470, 622
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 7 cut(s) 403, 644, 690, 905, 922, 946, 997
BtsIMutI CAGTG 3 cut(s) 129, 210, 942
Cac8I GCNNGC 4 cut(s) 477, 976, 1001, 1114
CciI TCATGA 3 cut(s) 217, 376, 538
CfoI GCGC 3 cut(s) 800, 850, 857
Cfr13I GGNCC 3 cut(s) 43, 44, 98
Cfr9I CCCGGG 1 cut(s) 187
Csp6I GTAC 1 cut(s) 522
CspCI CAANNNNNGTGG 2 cut(s) 775, 810
CviAII CATG 8 cut(s) 95, 218, 377, 410, 466, 539, 931, 1070
CviQI GTAC 1 cut(s) 522
DdeI CTNAG 4 cut(s) 140, 164, 291, 924
DpnI GATC 7 cut(s) 115, 216, 414, 729, 744, 873, 969
DpnII GATC 7 cut(s) 113, 214, 412, 727, 742, 871, 967
DrdI GACNNNNNNGTC 1 cut(s) 372
DseDI GACNNNNNNGTC 1 cut(s) 372
EaeI YGGCCR 1 cut(s) 468
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
EciI GGCGGA 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 426
Eco24I GRGCYC 1 cut(s) 47
Eco31I GGTCTC 1 cut(s) 82
Eco32I GATATC 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 98
Eco57I CTGAAG 2 cut(s) 341, 434
Eco88I CYCGRG 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 100, 393
EcoRV GATATC 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 426
EcoT22I ATGCAT 1 cut(s) 646
EcoT38I GRGCYC 1 cut(s) 47
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 8 cut(s) 98, 221, 380, 413, 469, 542, 934, 1073
FalI AAGNNNNNCTT 1 cut(s) 30
FaqI GGGAC 2 cut(s) 1007, 1029
FatI CATG 8 cut(s) 94, 217, 376, 409, 465, 538, 930, 1069
FbaI TGATCA 3 cut(s) 214, 412, 742
Fnu4HI GCNGC 2 cut(s) 270, 1062
FokI GGATG 7 cut(s) 410, 631, 697, 892, 929, 953, 1004
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 2 cut(s) 270, 1062
FspBI CTAG 4 cut(s) 606, 612, 813, 837
GlaI GCGC 3 cut(s) 799, 849, 856
GluI GCNGC 2 cut(s) 270, 1062
GsuI CTGGAG 2 cut(s) 39, 1058
HaeII RGCGCY 1 cut(s) 801
HaeIII GGCC 4 cut(s) 10, 45, 470, 622
HapII CCGG 2 cut(s) 188, 869
HhaI GCGC 3 cut(s) 800, 850, 857
Hin1II CATG 8 cut(s) 98, 221, 380, 413, 469, 542, 934, 1073
Hin6I GCGC 3 cut(s) 798, 848, 855
HinP1I GCGC 3 cut(s) 798, 848, 855
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 2 cut(s) 52, 884
HpaII CCGG 2 cut(s) 188, 869
Hpy166II GTNNAC 1 cut(s) 51
Hpy188I TCNGA 7 cut(s) 294, 360, 453, 727, 732, 747, 916
Hpy188III TCNNGA 6 cut(s) 56, 89, 218, 377, 539, 989
Hpy8I GTNNAC 1 cut(s) 51
HpyAV CCTTC 5 cut(s) 34, 438, 554, 766, 836
HpyCH4III ACNGT 1 cut(s) 532
HpyCH4IV ACGT 1 cut(s) 638
HpyCH4V TGCA 8 cut(s) 147, 159, 553, 644, 806, 953, 1064, 1112
HpyF10VI GCNNNNNNNGC 6 cut(s) 275, 352, 476, 854, 971, 1070
HpyF3I CTNAG 4 cut(s) 140, 164, 291, 924
HpySE526I ACGT 1 cut(s) 638
Hsp92II CATG 8 cut(s) 98, 221, 380, 413, 469, 542, 934, 1073
HspAI GCGC 3 cut(s) 798, 848, 855
Ksp22I TGATCA 3 cut(s) 214, 412, 742
Kzo9I GATC 7 cut(s) 113, 214, 412, 727, 742, 871, 967
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 1053
Lsp1109I GCAGC 2 cut(s) 281, 1048
LweI GCATC 3 cut(s) 134, 653, 1048
MaeI CTAG 4 cut(s) 606, 612, 813, 837
MaeII ACGT 1 cut(s) 638
MaeIII GTNAC 2 cut(s) 20, 203
MalI GATC 7 cut(s) 115, 216, 414, 729, 744, 873, 969
MbiI CCGCTC 1 cut(s) 710
MboI GATC 7 cut(s) 113, 214, 412, 727, 742, 871, 967
MboII GAAGA 6 cut(s) 49, 74, 238, 242, 278, 1091
MhlI GDGCHC 2 cut(s) 47, 833
MlsI TGGCCA 1 cut(s) 470
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 146
Mox20I TGGCCA 1 cut(s) 470
Mph1103I ATGCAT 1 cut(s) 646
MscI TGGCCA 1 cut(s) 470
MseI TTAA 2 cut(s) 573, 594
MslI CAYNNNNRTG 2 cut(s) 396, 935
Msp20I TGGCCA 1 cut(s) 470
MspI CCGG 2 cut(s) 188, 869
MspR9I CCNGG 4 cut(s) 102, 188, 189, 395
MvaI CCWGG 2 cut(s) 102, 395
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 6 cut(s) 275, 352, 476, 854, 971, 1070
NciI CCSGG 2 cut(s) 188, 189
NdeII GATC 7 cut(s) 113, 214, 412, 727, 742, 871, 967
NlaIII CATG 8 cut(s) 98, 221, 380, 413, 469, 542, 934, 1073
NlaIV GGNNCC 2 cut(s) 45, 1049
NmuCI GTSAC 1 cut(s) 20
NsiI ATGCAT 1 cut(s) 646
PagI TCATGA 3 cut(s) 217, 376, 538
PciSI GCTCTTC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 884
PkrI GCNGC 2 cut(s) 271, 1063
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 639
PshBI ATTAAT 2 cut(s) 573, 594
Psp6I CCWGG 2 cut(s) 100, 393
PspGI CCWGG 2 cut(s) 100, 393
PspN4I GGNNCC 2 cut(s) 45, 1049
PspOMI GGGCCC 1 cut(s) 43
PspPI GGNCC 3 cut(s) 43, 44, 98
RsaI GTAC 1 cut(s) 523
RsaNI GTAC 1 cut(s) 522
RseI CAYNNNNRTG 2 cut(s) 396, 935
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 2 cut(s) 573, 594
SatI GCNGC 2 cut(s) 270, 1062
Sau3AI GATC 7 cut(s) 113, 214, 412, 727, 742, 871, 967
Sau96I GGNCC 3 cut(s) 43, 44, 98
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 4 cut(s) 102, 188, 189, 395
SduI GDGCHC 2 cut(s) 47, 833
SfaNI GCATC 3 cut(s) 134, 653, 1048
SinI GGWCC 1 cut(s) 98
SmaI CCCGGG 1 cut(s) 189
SmiMI CAYNNNNRTG 2 cut(s) 396, 935
SsiI CCGC 3 cut(s) 184, 201, 708
SspI AATATT 1 cut(s) 592
SspMI CTAG 4 cut(s) 606, 612, 813, 837
StyD4I CCNGG 4 cut(s) 100, 186, 187, 393
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 1 cut(s) 532
TaiI ACGT 1 cut(s) 641
TaqI TCGA 2 cut(s) 675, 882
TfiI GAWTC 1 cut(s) 884
Tru1I TTAA 2 cut(s) 573, 594
Tru9I TTAA 2 cut(s) 573, 594
TscAI CASTG 3 cut(s) 136, 217, 942
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 2 cut(s) 269, 1061
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 3 cut(s) 270, 701, 949
TspMI CCCGGG 1 cut(s) 187
TspRI CASTG 3 cut(s) 136, 217, 942
VpaK11BI GGWCC 1 cut(s) 98
VspI ATTAAT 2 cut(s) 573, 594
XapI RAATTY 2 cut(s) 691, 955
XmaI CCCGGG 1 cut(s) 187
XspI CTAG 4 cut(s) 606, 612, 813, 837
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.