RchiOBHm_Chr7g0181361
MYB Family

Myb-related protein 306-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0009059
Physical Location & Seq
Forward (+)
2979355 .. 2981670
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16175

Sequence Viewer

Length: 1065 bp
ATGGGAAGACCACCTTGCTGTGACAAAGTTGGAGTGAAAAAAGGGCCATGGACGCCGGAGGAAGACATCATCTTGGTGTCGTACATTCAAGAACACGGCCCTGGGAATTGGAGATCAGTTCCTACCAACACAGGTTTGATGAGATGCAGTAAGAGCTGCCGGCTTAGATGGACTAATTATCTCCGGCCGGGCATCAAACGCGGTAACTTCACCGACCATGAGGAGAAGATGATCATCCACCTCCAAGCTCTTCTAGGCAATAGATGGGCTGCCATAGCTTCCTACCTTCCTCAGAGAACAGACAATGATATAAAGAATTACTGGAACACCCACCTGAAGAAGAAACTCAGAAAGCTCCAGACAGGTACTGCTGAAGACGGTATTTCAGGTAATTCACAGCAGCCAATTTCAAAGGGTCAGTGGGAGAGAAGGCTCCAAACAGACATTCACATGGCCAAACAAGCCCTTTGTGAGGCTCTCTCCCTTGACAAACCAAGTCATGATCATCACCATGTGCCAGACTTAAAGCCCTCACTCTCCAATATTAATGGCACTACTAGTACTACTACTAGACCTCACCAAGCATCCACATACGCGTCCAACACCGAAAACATTGCTCGATTGCTCGAAAACTGGATGAAAAGTTCACCAAAAGTTCGAACAAACTCCTCGGATGATCATCAAACCCAGATTCATCATCAGAACATGCTCAACAATGGTTCCAGTTCTAGTACTACCGACGGGGCACACAGTGGGACAACAACTACACCGGACCATCATGAACACCATGGTTTTGATTCCTTGTTTAGTAGCTTCAACTCATCCACATTATCCGATAATGCGTCGACTCATTTATCAGCAGAAAATAATCATCGTCAAACTAGTACTACTACTAATAATAATAATACTGGTCTCATGATGTTCCAAGACGAAAGCAAGCCTGATTTAGAGGCGGCCGCGGGAGCAGCAGCAGCAGTAGATCAAGTGCCTCTCACATTGCTGGAGAAGTGGCTCTTTGATGACGCTACTGCACATCAAGCTCATGATCAAGACATGCTGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009751 GO:0009753 GO:0009787 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010038 GO:0010115 GO:0010166 GO:0010468 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0014070 GO:0019216 GO:0019219 GO:0019222 GO:0019747 GO:0023051 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0033993 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043455 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046677 GO:0046686 GO:0046890 GO:0048518 GO:0048522 GO:0048580 GO:0048583 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0060255 GO:0062012 GO:0065007 GO:0071704 GO:0080050 GO:0080090 GO:0080167 GO:0097159 GO:0097305 GO:0140110 GO:1901363 GO:1901419 GO:1901568 GO:1901570 GO:1901576 GO:1901700 GO:1902680 GO:1902930 GO:1903506 GO:1903508 GO:1904276 GO:1904278 GO:1905957 GO:2000026 GO:2000033 GO:2000034 GO:2000112 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

354

Amino Acids

39.53

Weight (kDa)

6.74

Isoelectric Point (pI)

34.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 1.8e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 1.3e-10 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 1.7e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 70 - 118 2.8e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 845
AccII CGCG 3 cut(s) 201, 596, 959
AciI CCGC 4 cut(s) 201, 953, 957, 959
AcoI YGGCCR 3 cut(s) 185, 453, 954
AcuI CTGAAG 2 cut(s) 356, 393
AcyI GRCGYC 1 cut(s) 53
AdeI CACNNNGTG 2 cut(s) 514, 752
AfaI GTAC 5 cut(s) 83, 367, 562, 733, 886
AfiI CCNNNNNNNGG 1 cut(s) 472
AflIII ACRYGT 1 cut(s) 594
AgsI TTSAA 3 cut(s) 89, 411, 817
AhlI ACTAGT 2 cut(s) 557, 881
AjnI CCWGG 1 cut(s) 100
AjuI GAANNNNNNNTTGG 2 cut(s) 429, 461
AloI GAACNNNNNNTCC 2 cut(s) 628, 660
AluBI AGCT 6 cut(s) 156, 248, 278, 355, 813, 1040
AluI AGCT 6 cut(s) 156, 248, 278, 355, 813, 1040
Alw26I GTCTC 1 cut(s) 917
AlwNI CAGNNNCTG 1 cut(s) 368
AoxI GGCC 5 cut(s) 44, 97, 185, 453, 954
ApeKI GCWGC 6 cut(s) 156, 269, 400, 965, 968, 971
AseI ATTAAT 1 cut(s) 546
AspS9I GGNCC 3 cut(s) 44, 98, 772
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 4 cut(s) 202, 500, 569, 639
AsuII TTCGAA 1 cut(s) 658
AvaII GGWCC 1 cut(s) 772
BaeGI GKGCMC 1 cut(s) 748
BalI TGGCCA 1 cut(s) 455
BbsI GAAGAC 3 cut(s) 13, 69, 381
BbvI GCAGC 6 cut(s) 143, 256, 412, 977, 980, 983
BccI CCATC 3 cut(s) 162, 258, 783
BceAI ACGGC 1 cut(s) 112
BcgI CGANNNNNNTGC 2 cut(s) 596, 630
BciT130I CCWGG 1 cut(s) 102
BclI TGATCA 4 cut(s) 231, 502, 676, 1045
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 917
BcuI ACTAGT 2 cut(s) 557, 881
BfaI CTAG 5 cut(s) 254, 558, 570, 729, 882
BisI GCNGC 8 cut(s) 157, 270, 401, 954, 957, 966, 969, 972
BlsI GCNGC 8 cut(s) 158, 271, 402, 955, 958, 967, 970, 973
BmcAI AGTACT 3 cut(s) 562, 733, 886
Bme1390I CCNGG 2 cut(s) 102, 189
Bme18I GGWCC 1 cut(s) 772
BmgT120I GGNCC 3 cut(s) 44, 98, 772
BmiI GGNNCC 2 cut(s) 434, 721
BmrFI CCNGG 2 cut(s) 102, 189
BmsI GCATC 3 cut(s) 134, 201, 593
BpiI GAAGAC 3 cut(s) 13, 69, 381
BplI GAGNNNNNCTC 2 cut(s) 464, 496
BpmI CTGGAG 2 cut(s) 341, 1022
Bpu14I TTCGAA 1 cut(s) 658
BpuMI CCSGG 1 cut(s) 189
BsaBI GATNNNNATC 2 cut(s) 233, 678
BsaHI GRCGYC 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 917
BsaJI CCNNGG 6 cut(s) 47, 100, 101, 669, 787, 957
BsaWI WCCGGW 1 cut(s) 769
Bsc4I CCNNNNNNNGG 1 cut(s) 472
Bse118I RCCGGY 1 cut(s) 159
Bse1I ACTGG 4 cut(s) 326, 638, 723, 913
Bse3DI GCAATG 2 cut(s) 612, 995
Bse8I GATNNNNATC 2 cut(s) 233, 678
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 6 cut(s) 47, 100, 101, 669, 787, 957
BseGI GGATG 5 cut(s) 234, 584, 642, 679, 821
BseJI GATNNNNATC 2 cut(s) 233, 678
BseLI CCNNNNNNNGG 1 cut(s) 472
BseMI GCAATG 2 cut(s) 612, 995
BseMII CTCAG 2 cut(s) 305, 361
BseNI ACTGG 4 cut(s) 326, 638, 723, 913
BseRI GAGGAG 2 cut(s) 236, 658
BseSI GKGCMC 1 cut(s) 748
BseX3I CGGCCG 2 cut(s) 185, 954
BseXI GCAGC 6 cut(s) 143, 256, 412, 977, 980, 983
BsgI GTGCAG 1 cut(s) 1014
Bsh1236I CGCG 3 cut(s) 201, 596, 959
Bsh1285I CGRYCG 2 cut(s) 188, 957
BshFI GGCC 5 cut(s) 46, 99, 187, 455, 956
BsiEI CGRYCG 2 cut(s) 188, 957
BsiSI CCGG 5 cut(s) 56, 160, 184, 188, 770
BslFI GGGAC 1 cut(s) 769
BslI CCNNNNNNNGG 1 cut(s) 472
BsmAI GTCTC 1 cut(s) 917
BsmFI GGGAC 1 cut(s) 769
BsnI GGCC 5 cut(s) 46, 99, 187, 455, 956
Bso31I GGTCTC 1 cut(s) 917
Bsp119I TTCGAA 1 cut(s) 658
Bsp1286I GDGCHC 1 cut(s) 748
Bsp143I GATC 6 cut(s) 113, 231, 502, 676, 979, 1045
Bsp19I CCATGG 2 cut(s) 47, 787
BspACI CCGC 4 cut(s) 201, 953, 957, 959
BspANI GGCC 5 cut(s) 46, 99, 187, 455, 956
BspCNI CTCAG 2 cut(s) 304, 360
BspFNI CGCG 3 cut(s) 201, 596, 959
BspHI TCATGA 4 cut(s) 499, 778, 915, 1042
BspLI GGNNCC 2 cut(s) 434, 721
BspQI GCTCTTC 1 cut(s) 255
BspT104I TTCGAA 1 cut(s) 658
BspTNI GGTCTC 1 cut(s) 917
BsrDI GCAATG 2 cut(s) 612, 995
BsrFI RCCGGY 1 cut(s) 159
BsrI ACTGG 4 cut(s) 326, 638, 723, 913
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 6 cut(s) 47, 100, 101, 669, 787, 957
BssMI GATC 6 cut(s) 113, 231, 502, 676, 979, 1045
BssNI GRCGYC 1 cut(s) 53
BssT1I CCWWGG 2 cut(s) 47, 787
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 2 cut(s) 380, 752
Bst6I CTCTTC 1 cut(s) 255
BstACI GRCGYC 1 cut(s) 53
BstBI TTCGAA 1 cut(s) 658
BstC8I GCNNGC 2 cut(s) 161, 938
BstDEI CTNAG 3 cut(s) 164, 291, 347
BstDSI CCRYGG 3 cut(s) 47, 787, 957
BstENI CCTNNNNNAGG 1 cut(s) 470
BstF5I GGATG 5 cut(s) 234, 584, 642, 679, 821
BstFNI CGCG 3 cut(s) 201, 596, 959
BstKTI GATC 6 cut(s) 116, 234, 505, 679, 982, 1048
BstMAI GTCTC 1 cut(s) 917
BstMBI GATC 6 cut(s) 113, 231, 502, 676, 979, 1045
BstMCI CGRYCG 2 cut(s) 188, 957
BstMWI GCNNNNNNNGC 9 cut(s) 52, 153, 198, 275, 461, 962, 965, 971, 1037
BstNI CCWGG 1 cut(s) 102
BstNSI RCATGY 2 cut(s) 709, 1057
BstSCI CCNGG 2 cut(s) 100, 187
BstSLI GKGCMC 1 cut(s) 748
BstUI CGCG 3 cut(s) 201, 596, 959
BstV1I GCAGC 6 cut(s) 143, 256, 412, 977, 980, 983
BstV2I GAAGAC 3 cut(s) 13, 69, 381
BstZI CGGCCG 2 cut(s) 185, 954
BsuRI GGCC 5 cut(s) 46, 99, 187, 455, 956
BtgI CCRYGG 3 cut(s) 47, 787, 957
BtsCI GGATG 5 cut(s) 234, 584, 642, 679, 821
BtsIMutI CAGTG 2 cut(s) 425, 757
Cac8I GCNNGC 2 cut(s) 161, 938
CaiI CAGNNNCTG 1 cut(s) 368
CciI TCATGA 4 cut(s) 499, 778, 915, 1042
CciNI GCGGCCGC 1 cut(s) 954
Cfr10I RCCGGY 1 cut(s) 159
Cfr13I GGNCC 3 cut(s) 44, 98, 772
Cfr42I CCGCGG 1 cut(s) 960
CseI GACGC 4 cut(s) 61, 585, 831, 1031
Csp6I GTAC 5 cut(s) 82, 366, 561, 732, 885
CviQI GTAC 5 cut(s) 82, 366, 561, 732, 885
DdeI CTNAG 3 cut(s) 164, 291, 347
DpnI GATC 6 cut(s) 115, 233, 504, 678, 981, 1047
DpnII GATC 6 cut(s) 113, 231, 502, 676, 979, 1045
DraIII CACNNNGTG 2 cut(s) 514, 752
EaeI YGGCCR 3 cut(s) 185, 453, 954
EagI CGGCCG 2 cut(s) 185, 954
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
EclXI CGGCCG 2 cut(s) 185, 954
Eco130I CCWWGG 2 cut(s) 47, 787
Eco31I GGTCTC 1 cut(s) 917
Eco47I GGWCC 1 cut(s) 772
Eco52I CGGCCG 2 cut(s) 185, 954
Eco57I CTGAAG 2 cut(s) 356, 393
EcoNI CCTNNNNNAGG 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 100
EcoT14I CCWWGG 2 cut(s) 47, 787
ErhI CCWWGG 2 cut(s) 47, 787
FalI AAGNNNNNCTT 3 cut(s) 30, 998, 1030
FaqI GGGAC 1 cut(s) 769
FauI CCCGC 1 cut(s) 952
FbaI TGATCA 4 cut(s) 231, 502, 676, 1045
FblI GTMKAC 1 cut(s) 845
Fnu4HI GCNGC 8 cut(s) 157, 270, 401, 954, 957, 966, 969, 972
FokI GGATG 5 cut(s) 221, 571, 649, 686, 808
Fsp4HI GCNGC 8 cut(s) 157, 270, 401, 954, 957, 966, 969, 972
FspBI CTAG 5 cut(s) 254, 558, 570, 729, 882
GluI GCNGC 8 cut(s) 157, 270, 401, 954, 957, 966, 969, 972
GsuI CTGGAG 2 cut(s) 341, 1022
HaeIII GGCC 5 cut(s) 46, 99, 187, 455, 956
HapII CCGG 5 cut(s) 56, 160, 184, 188, 770
HgaI GACGC 4 cut(s) 61, 585, 831, 1031
Hin1I GRCGYC 1 cut(s) 53
HincII GTYRAC 1 cut(s) 846
HindII GTYRAC 1 cut(s) 846
HinfI GANTC 3 cut(s) 691, 797, 847
HpaII CCGG 5 cut(s) 56, 160, 184, 188, 770
HphI GGTGA 4 cut(s) 202, 500, 569, 639
Hpy166II GTNNAC 2 cut(s) 647, 846
Hpy188I TCNGA 5 cut(s) 294, 350, 673, 702, 835
Hpy188III TCNNGA 7 cut(s) 89, 358, 500, 779, 916, 1043, 1049
Hpy8I GTNNAC 2 cut(s) 647, 846
Hpy99I CGWCG 2 cut(s) 743, 847
HpyAV CCTTC 2 cut(s) 296, 423
HpyCH4III ACNGT 2 cut(s) 380, 752
HpyCH4V TGCA 2 cut(s) 147, 1031
HpyF10VI GCNNNNNNNGC 9 cut(s) 52, 153, 198, 275, 461, 962, 965, 971, 1037
HpyF3I CTNAG 3 cut(s) 164, 291, 347
Hsp92I GRCGYC 1 cut(s) 53
KroI GCCGGC 1 cut(s) 159
KroNI GCCGGC 1 cut(s) 161
Ksp22I TGATCA 4 cut(s) 231, 502, 676, 1045
KspI CCGCGG 1 cut(s) 960
Kzo9I GATC 6 cut(s) 113, 231, 502, 676, 979, 1045
LguI GCTCTTC 1 cut(s) 255
LmnI GCTCC 3 cut(s) 360, 438, 962
Lsp1109I GCAGC 6 cut(s) 143, 256, 412, 977, 980, 983
LweI GCATC 3 cut(s) 134, 201, 593
MaeI CTAG 5 cut(s) 254, 558, 570, 729, 882
MaeIII GTNAC 2 cut(s) 20, 203
MalI GATC 6 cut(s) 115, 233, 504, 678, 981, 1047
MboI GATC 6 cut(s) 113, 231, 502, 676, 979, 1045
MboII GAAGA 7 cut(s) 18, 74, 238, 242, 349, 352, 386
MhlI GDGCHC 1 cut(s) 748
MlsI TGGCCA 1 cut(s) 455
MluCI AATT 5 cut(s) 106, 175, 316, 391, 405
MluI ACGCGT 1 cut(s) 594
MluNI TGGCCA 1 cut(s) 455
MlyI GAGTC 1 cut(s) 841
MmeI TCCRAC 2 cut(s) 10, 624
Mox20I TGGCCA 1 cut(s) 455
MroNI GCCGGC 1 cut(s) 159
MscI TGGCCA 1 cut(s) 455
MseI TTAA 3 cut(s) 524, 546, 1063
MslI CAYNNNNRTG 3 cut(s) 74, 449, 510
Msp20I TGGCCA 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 5 cut(s) 56, 160, 184, 188, 770
MspR9I CCNGG 2 cut(s) 102, 189
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 3 cut(s) 201, 596, 959
MwoI GCNNNNNNNGC 9 cut(s) 52, 153, 198, 275, 461, 962, 965, 971, 1037
NaeI GCCGGC 1 cut(s) 161
NciI CCSGG 1 cut(s) 189
NcoI CCATGG 2 cut(s) 47, 787
NdeII GATC 6 cut(s) 113, 231, 502, 676, 979, 1045
NgoMIV GCCGGC 1 cut(s) 159
NlaIV GGNNCC 2 cut(s) 434, 721
NmuCI GTSAC 1 cut(s) 20
NotI GCGGCCGC 1 cut(s) 954
NspI RCATGY 2 cut(s) 709, 1057
NspV TTCGAA 1 cut(s) 658
PagI TCATGA 4 cut(s) 499, 778, 915, 1042
PasI CCCWGGG 1 cut(s) 101
PciSI GCTCTTC 1 cut(s) 255
PdiI GCCGGC 1 cut(s) 161
PfeI GAWTC 2 cut(s) 691, 797
PkrI GCNGC 8 cut(s) 158, 271, 402, 955, 958, 967, 970, 973
PleI GAGTC 1 cut(s) 841
PpsI GAGTC 1 cut(s) 841
PshBI ATTAAT 1 cut(s) 546
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 434, 721
PspPI GGNCC 3 cut(s) 44, 98, 772
PstNI CAGNNNCTG 1 cut(s) 368
RsaI GTAC 5 cut(s) 83, 367, 562, 733, 886
RsaNI GTAC 5 cut(s) 82, 366, 561, 732, 885
RseI CAYNNNNRTG 3 cut(s) 74, 449, 510
SacII CCGCGG 1 cut(s) 960
SalI GTCGAC 1 cut(s) 844
SapI GCTCTTC 1 cut(s) 255
SaqAI TTAA 3 cut(s) 524, 546, 1063
SatI GCNGC 8 cut(s) 157, 270, 401, 954, 957, 966, 969, 972
Sau3AI GATC 6 cut(s) 113, 231, 502, 676, 979, 1045
Sau96I GGNCC 3 cut(s) 44, 98, 772
ScaI AGTACT 3 cut(s) 562, 733, 886
SchI GAGTC 1 cut(s) 841
ScrFI CCNGG 2 cut(s) 102, 189
SduI GDGCHC 1 cut(s) 748
SfaNI GCATC 3 cut(s) 134, 201, 593
Sfr303I CCGCGG 1 cut(s) 960
SfuI TTCGAA 1 cut(s) 658
SgrBI CCGCGG 1 cut(s) 960
SinI GGWCC 1 cut(s) 772
SmiMI CAYNNNNRTG 3 cut(s) 74, 449, 510
SpeI ACTAGT 2 cut(s) 557, 881
Sse9I AATT 5 cut(s) 106, 175, 316, 391, 405
SsiI CCGC 4 cut(s) 201, 953, 957, 959
SspI AATATT 1 cut(s) 544
SspMI CTAG 5 cut(s) 254, 558, 570, 729, 882
StyD4I CCNGG 2 cut(s) 100, 187
StyI CCWWGG 2 cut(s) 47, 787
TaaI ACNGT 2 cut(s) 380, 752
TaqI TCGA 4 cut(s) 619, 627, 658, 845
TasI AATT 5 cut(s) 106, 175, 316, 391, 405
TatI WGTACW 3 cut(s) 560, 731, 884
TauI GCSGC 2 cut(s) 956, 959
TfiI GAWTC 2 cut(s) 691, 797
Tru1I TTAA 3 cut(s) 524, 546, 1063
Tru9I TTAA 3 cut(s) 524, 546, 1063
TscAI CASTG 2 cut(s) 425, 757
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 6 cut(s) 156, 269, 400, 965, 968, 971
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 3 cut(s) 653, 683, 795
TspRI CASTG 2 cut(s) 425, 757
VpaK11BI GGWCC 1 cut(s) 772
VspI ATTAAT 1 cut(s) 546
XagI CCTNNNNNAGG 1 cut(s) 470
XceI RCATGY 2 cut(s) 709, 1057
XmiI GTMKAC 1 cut(s) 845
XspI CTAG 5 cut(s) 254, 558, 570, 729, 882
ZrmI AGTACT 3 cut(s) 562, 733, 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.