MD14G1200100.v1.1
MYB Family

Myb-related protein 306-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009059
Physical Location & Seq
Forward (+)
28949738 .. 28953384
3647 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1200100.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGGGAAGGCCACCTTGCTGTGACAAACTTGGTGTGAAGAAAGGGCCATGGACTCCAGAGGAAGACATCATATTGGTTTCTTACATTCAAGAACATGGTCCTGGGAATTGGAGATCAGTTCCTACTAACACTGGTTTGCTGAGATGCAGTAAAAGCTGCAGACTTAGATGGACTAATTATCTCCGCTCGGGTATCAAACGCGGTAACTTCACCGATCATGAGGAGAAGATGATAATCCATCTCCAAGCTCTTTTGGGCAATAGATGGGCAGCCATAGCTTCATACCTTCCTCAGAGAACAGACAATGACATCAAAAATTACTGGAACACTCACTTGAAAAAAAAGCTGAAAAAGCTCCAGACAGGGCTTGATGGTCATGACCATCACAACAACCAGGATGGTTTTTCAGGTCATTCACATGATCAGCCAAATTCCAAGGGTCAGTGGGAGAGAAGGCTTCAGACAGATATCCACATGGCCAAGCAAGCTCTTTCTGAGGCTCTCTCCATTGGCAAACCAAGTATTATTAGTACTAGTGCTACTACTTCTCATGATCTTCACTTGCAAGACTTGAAGCCCTCCATTAATATTGGTAATTACAATATTAATGATAACCCTAGCACTAGCACAAGGCCTCACCAAGCCTCCACGTACGCATCCAACACCGATAGCATTGCAAAATTGCTCGAAAACTGGATGAAAAATTCGCCGAAGGTTTCCACTCATCAAAAAAACTCGGATCAGACCAATGCTGTTCCGATCAATTCCTTCAATAACAACAACAAAGGTGGCACTACAAGAGGTTGTTCAATGAGTACTTCTAGTGAAGGGGCGCACAGCGCAACCACTACGCCTGATCAGGCTTTCGATTCGTTGATCAGCTTCAACTCATCCCCATCTGATGTGTCTCAGTCCATGTCAGTGGATGAGAACAATGCAAATTTCACAGCAGATCAGACAAGCTGTCTGTTTCAGGATGAGAGCAAGCCCAATTTGGAGGGACAAGTCCCTCTCACATTGCTGGAGAAGTGGCTCTTTGATGATGCTGCACCTCATGCCCACGAAGACCTAATTAACATGTCATTAGAGAATGGTGCAGGCTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009751 GO:0009753 GO:0009787 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010038 GO:0010115 GO:0010166 GO:0010468 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0014070 GO:0019216 GO:0019219 GO:0019222 GO:0019747 GO:0023051 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0033993 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043455 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046677 GO:0046686 GO:0046890 GO:0048518 GO:0048522 GO:0048580 GO:0048583 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0060255 GO:0062012 GO:0065007 GO:0071704 GO:0080050 GO:0080090 GO:0080167 GO:0097159 GO:0097305 GO:0140110 GO:1901363 GO:1901419 GO:1901568 GO:1901570 GO:1901576 GO:1901700 GO:1902680 GO:1902930 GO:1903506 GO:1903508 GO:1904276 GO:1904278 GO:1905957 GO:2000026 GO:2000033 GO:2000034 GO:2000112 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

370

Amino Acids

40.94

Weight (kDa)

6.62

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 2.1e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 6.4e-10 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 1.8e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 70 - 118 3e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 186
AccII CGCG 1 cut(s) 201
AciI CCGC 2 cut(s) 184, 201
AclWI GGATC 1 cut(s) 747
AcoI YGGCCR 1 cut(s) 477
AcsI RAATTY 3 cut(s) 430, 703, 940
AcuI CTGAAG 1 cut(s) 443
AfaI GTAC 3 cut(s) 532, 653, 817
AflIII ACRYGT 1 cut(s) 1077
AgsI TTSAA 6 cut(s) 89, 337, 574, 772, 810, 886
AhdI GACNNNNNGTC 1 cut(s) 963
AhlI ACTAGT 1 cut(s) 533
AjnI CCWGG 2 cut(s) 100, 393
AluBI AGCT 8 cut(s) 156, 248, 278, 346, 355, 488, 882, 963
AluI AGCT 8 cut(s) 156, 248, 278, 346, 355, 488, 882, 963
Alw26I GTCTC 1 cut(s) 912
AlwI GGATC 1 cut(s) 747
Ama87I CYCGRG 1 cut(s) 187
AoxI GGCC 4 cut(s) 8, 44, 477, 632
ApeKI GCWGC 3 cut(s) 156, 269, 1046
ApoI RAATTY 3 cut(s) 430, 703, 940
AseI ATTAAT 2 cut(s) 585, 606
AspLEI GCGC 2 cut(s) 835, 842
AspS9I GGNCC 2 cut(s) 44, 98
AsuHPI GGTGA 2 cut(s) 202, 629
AvaI CYCGRG 1 cut(s) 187
AvaII GGWCC 1 cut(s) 98
BalI TGGCCA 1 cut(s) 479
BbsI GAAGAC 2 cut(s) 69, 1071
BbvI GCAGC 3 cut(s) 143, 281, 1033
BccI CCATC 7 cut(s) 162, 246, 258, 365, 390, 392, 904
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 2 cut(s) 102, 395
BclI TGATCA 3 cut(s) 421, 856, 876
BcoDI GTCTC 1 cut(s) 912
BcuI ACTAGT 1 cut(s) 533
BfaI CTAG 4 cut(s) 534, 618, 624, 822
BfmI CTRYAG 1 cut(s) 157
BisI GCNGC 3 cut(s) 157, 270, 1047
BlsI GCNGC 3 cut(s) 158, 271, 1048
BmcAI AGTACT 2 cut(s) 532, 817
Bme1390I CCNGG 2 cut(s) 102, 395
Bme18I GGWCC 1 cut(s) 98
BmeRI GACNNNNNGTC 1 cut(s) 963
BmeT110I CYCGRG 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 44, 98
BmrFI CCNGG 2 cut(s) 102, 395
BmsI GCATC 3 cut(s) 134, 665, 1033
BpiI GAAGAC 2 cut(s) 69, 1071
BplI GAGNNNNNCTC 2 cut(s) 488, 520
BpmI CTGGAG 3 cut(s) 39, 341, 1043
BsaAI YACGTR 1 cut(s) 651
BsaBI GATNNNNATC 1 cut(s) 233
BsaJI CCNNGG 3 cut(s) 47, 101, 435
BsaXI ACNNNNNCTCC 2 cut(s) 629, 659
Bse1I ACTGG 3 cut(s) 136, 326, 698
Bse3DI GCAATG 2 cut(s) 672, 1016
Bse8I GATNNNNATC 1 cut(s) 233
BseBI CCWGG 2 cut(s) 102, 395
BseDI CCNNGG 3 cut(s) 47, 101, 435
BseGI GGATG 6 cut(s) 403, 656, 702, 890, 931, 982
BseJI GATNNNNATC 1 cut(s) 233
BseMI GCAATG 2 cut(s) 672, 1016
BseMII CTCAG 4 cut(s) 131, 305, 486, 923
BseNI ACTGG 3 cut(s) 136, 326, 698
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 3 cut(s) 143, 281, 1033
BsgI GTGCAG 1 cut(s) 1032
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 4 cut(s) 10, 46, 479, 634
BsiHKCI CYCGRG 1 cut(s) 187
BsiWI CGTACG 1 cut(s) 651
BslFI GGGAC 2 cut(s) 992, 1014
BsmAI GTCTC 1 cut(s) 912
BsmFI GGGAC 2 cut(s) 992, 1014
BsnI GGCC 4 cut(s) 10, 46, 479, 634
BsoBI CYCGRG 1 cut(s) 187
Bsp143I GATC 9 cut(s) 113, 214, 421, 553, 739, 759, 856, 876, 952
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 2 cut(s) 184, 201
BspANI GGCC 4 cut(s) 10, 46, 479, 634
BspCNI CTCAG 4 cut(s) 132, 304, 487, 922
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 3 cut(s) 217, 376, 550
BspMAI CTGCAG 1 cut(s) 161
BspPI GGATC 1 cut(s) 747
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 2 cut(s) 672, 1016
BsrI ACTGG 3 cut(s) 136, 326, 698
BssECI CCNNGG 3 cut(s) 47, 101, 435
BssMI GATC 9 cut(s) 113, 214, 421, 553, 739, 759, 856, 876, 952
BssT1I CCWWGG 2 cut(s) 47, 435
Bst2UI CCWGG 2 cut(s) 102, 395
BstAPI GCANNNNNTGC 1 cut(s) 1055
BstBAI YACGTR 1 cut(s) 651
BstC8I GCNNGC 3 cut(s) 486, 986, 1099
BstDEI CTNAG 5 cut(s) 140, 164, 291, 495, 909
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 6 cut(s) 403, 656, 702, 890, 931, 982
BstFNI CGCG 1 cut(s) 201
BstHHI GCGC 2 cut(s) 835, 842
BstKTI GATC 9 cut(s) 116, 217, 424, 556, 742, 762, 859, 879, 955
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 9 cut(s) 113, 214, 421, 553, 739, 759, 856, 876, 952
BstMWI GCNNNNNNNGC 6 cut(s) 153, 275, 352, 485, 839, 1055
BstNI CCWGG 2 cut(s) 102, 395
BstNSI RCATGY 1 cut(s) 1081
BstSCI CCNGG 2 cut(s) 100, 393
BstSFI CTRYAG 1 cut(s) 157
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 3 cut(s) 143, 281, 1033
BstV2I GAAGAC 2 cut(s) 69, 1071
BstXI CCANNNNNNTGG 1 cut(s) 922
BsuRI GGCC 4 cut(s) 10, 46, 479, 634
BtgI CCRYGG 1 cut(s) 47
BtsCI GGATG 6 cut(s) 403, 656, 702, 890, 931, 982
BtsIMutI CAGTG 3 cut(s) 129, 449, 927
Cac8I GCNNGC 3 cut(s) 486, 986, 1099
CciI TCATGA 3 cut(s) 217, 376, 550
CfoI GCGC 2 cut(s) 835, 842
Cfr13I GGNCC 2 cut(s) 44, 98
Csp6I GTAC 3 cut(s) 531, 652, 816
CspCI CAANNNNNGTGG 2 cut(s) 769, 804
CviQI GTAC 3 cut(s) 531, 652, 816
DdeI CTNAG 5 cut(s) 140, 164, 291, 495, 909
DpnI GATC 9 cut(s) 115, 216, 423, 555, 741, 761, 858, 878, 954
DpnII GATC 9 cut(s) 113, 214, 421, 553, 739, 759, 856, 876, 952
DriI GACNNNNNGTC 1 cut(s) 963
EaeI YGGCCR 1 cut(s) 477
Eam1105I GACNNNNNGTC 1 cut(s) 963
Eco130I CCWWGG 2 cut(s) 47, 435
Eco147I AGGCCT 1 cut(s) 634
Eco32I GATATC 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 98
Eco57I CTGAAG 1 cut(s) 443
Eco88I CYCGRG 1 cut(s) 187
EcoRII CCWGG 2 cut(s) 100, 393
EcoRV GATATC 1 cut(s) 469
EcoT14I CCWWGG 2 cut(s) 47, 435
ErhI CCWWGG 2 cut(s) 47, 435
FalI AAGNNNNNCTT 3 cut(s) 30, 1019, 1051
FaqI GGGAC 2 cut(s) 992, 1014
FbaI TGATCA 3 cut(s) 421, 856, 876
Fnu4HI GCNGC 3 cut(s) 157, 270, 1047
FokI GGATG 6 cut(s) 410, 643, 709, 877, 938, 989
Fsp4HI GCNGC 3 cut(s) 157, 270, 1047
FspBI CTAG 4 cut(s) 534, 618, 624, 822
GlaI GCGC 2 cut(s) 834, 841
GluI GCNGC 3 cut(s) 157, 270, 1047
GsuI CTGGAG 3 cut(s) 39, 341, 1043
HaeIII GGCC 4 cut(s) 10, 46, 479, 634
HhaI GCGC 2 cut(s) 835, 842
Hin6I GCGC 2 cut(s) 833, 840
HinP1I GCGC 2 cut(s) 833, 840
HinfI GANTC 2 cut(s) 52, 869
HphI GGTGA 2 cut(s) 202, 629
Hpy188I TCNGA 8 cut(s) 294, 462, 496, 739, 744, 759, 901, 957
Hpy188III TCNNGA 7 cut(s) 56, 89, 218, 358, 377, 551, 974
HpyAV CCTTC 5 cut(s) 296, 447, 706, 778, 821
HpyCH4IV ACGT 1 cut(s) 650
HpyCH4V TGCA 7 cut(s) 147, 159, 565, 677, 938, 1049, 1097
HpyF10VI GCNNNNNNNGC 6 cut(s) 153, 275, 352, 485, 839, 1055
HpyF3I CTNAG 5 cut(s) 140, 164, 291, 495, 909
HpySE526I ACGT 1 cut(s) 650
HspAI GCGC 2 cut(s) 833, 840
Ksp22I TGATCA 3 cut(s) 421, 856, 876
Kzo9I GATC 9 cut(s) 113, 214, 421, 553, 739, 759, 856, 876, 952
LmnI GCTCC 1 cut(s) 360
Lsp1109I GCAGC 3 cut(s) 143, 281, 1033
LweI GCATC 3 cut(s) 134, 665, 1033
MaeI CTAG 4 cut(s) 534, 618, 624, 822
MaeII ACGT 1 cut(s) 650
MaeIII GTNAC 2 cut(s) 20, 203
MalI GATC 9 cut(s) 115, 216, 423, 555, 741, 761, 858, 878, 954
MbiI CCGCTC 1 cut(s) 186
MboI GATC 9 cut(s) 113, 214, 421, 553, 739, 759, 856, 876, 952
MboII GAAGA 5 cut(s) 49, 74, 238, 548, 1076
MlsI TGGCCA 1 cut(s) 479
MluNI TGGCCA 1 cut(s) 479
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 684
Mox20I TGGCCA 1 cut(s) 479
MscI TGGCCA 1 cut(s) 479
MseI TTAA 3 cut(s) 585, 606, 1074
MslI CAYNNNNRTG 2 cut(s) 417, 920
Msp20I TGGCCA 1 cut(s) 479
MspR9I CCNGG 2 cut(s) 102, 395
MvaI CCWGG 2 cut(s) 102, 395
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 6 cut(s) 153, 275, 352, 485, 839, 1055
NcoI CCATGG 1 cut(s) 47
NdeII GATC 9 cut(s) 113, 214, 421, 553, 739, 759, 856, 876, 952
NmuCI GTSAC 1 cut(s) 20
NspI RCATGY 1 cut(s) 1081
PagI TCATGA 3 cut(s) 217, 376, 550
PceI AGGCCT 1 cut(s) 634
PciI ACATGT 1 cut(s) 1077
PfeI GAWTC 1 cut(s) 869
Pfl23II CGTACG 1 cut(s) 651
PkrI GCNGC 3 cut(s) 158, 271, 1048
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 651
PscI ACATGT 1 cut(s) 1077
PshBI ATTAAT 2 cut(s) 585, 606
Psp6I CCWGG 2 cut(s) 100, 393
PspGI CCWGG 2 cut(s) 100, 393
PspLI CGTACG 1 cut(s) 651
PspPI GGNCC 2 cut(s) 44, 98
PstI CTGCAG 1 cut(s) 161
RsaI GTAC 3 cut(s) 532, 653, 817
RsaNI GTAC 3 cut(s) 531, 652, 816
RseI CAYNNNNRTG 2 cut(s) 417, 920
SaqAI TTAA 3 cut(s) 585, 606, 1074
SatI GCNGC 3 cut(s) 157, 270, 1047
Sau3AI GATC 9 cut(s) 113, 214, 421, 553, 739, 759, 856, 876, 952
Sau96I GGNCC 2 cut(s) 44, 98
ScaI AGTACT 2 cut(s) 532, 817
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 2 cut(s) 102, 395
SfaNI GCATC 3 cut(s) 134, 665, 1033
SfcI CTRYAG 1 cut(s) 157
SinI GGWCC 1 cut(s) 98
SmiMI CAYNNNNRTG 2 cut(s) 417, 920
SpeI ACTAGT 1 cut(s) 533
SseBI AGGCCT 1 cut(s) 634
SsiI CCGC 2 cut(s) 184, 201
SspI AATATT 2 cut(s) 589, 604
SspMI CTAG 4 cut(s) 534, 618, 624, 822
StuI AGGCCT 1 cut(s) 634
StyD4I CCNGG 2 cut(s) 100, 393
StyI CCWWGG 2 cut(s) 47, 435
TaiI ACGT 1 cut(s) 653
TaqI TCGA 2 cut(s) 687, 867
TatI WGTACW 2 cut(s) 530, 815
TfiI GAWTC 1 cut(s) 869
Tru1I TTAA 3 cut(s) 585, 606, 1074
Tru9I TTAA 3 cut(s) 585, 606, 1074
TscAI CASTG 3 cut(s) 136, 449, 927
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 3 cut(s) 156, 269, 1046
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 2 cut(s) 270, 713
TspRI CASTG 3 cut(s) 136, 449, 927
VpaK11BI GGWCC 1 cut(s) 98
VspI ATTAAT 2 cut(s) 585, 606
XapI RAATTY 3 cut(s) 430, 703, 940
XceI RCATGY 1 cut(s) 1081
XspI CTAG 4 cut(s) 534, 618, 624, 822
ZrmI AGTACT 2 cut(s) 532, 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.