MD06G1228400.v1.1

Probably involved in the biogenesis of the COX complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009259
Physical Location & Seq
Reverse (-)
35843921 .. 35846818
2898 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1228400.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGCAGCAAAAACCTCCATTGCCAAAACCATAACCAAGCTTTATTATTCTAGCTGGTCCCATTCTTCTCACCCCAAACATCTGGCCCCACTATCACTCTCTTCTTCCTTCTCCAGCTCCTCCCCTGCCGATGCTTCCTCAGCAGCCGAGTCTCAATCACCCATCTCTTCACAATCTCCAGGGAAGGAAAGGTCGAGATTGTCGAGATGGTTGCTGTTTCTTCCTGGAGCTATCACTTTTGGCCTTGGGACTTGGCAGATTATCAGAAGGCAAGATAAGATAAAAATGTTAGATTATAGACGGAAGAGGTTGGAATTGGAACCTTTAAACTTGAGCAATGCCTCTCCGTCAAGTCAAGAATTGGATCAGTTGGAGTTTAGGAGGGTGAAATGCAAAGGTTATTTTGATGAGAAAAGGTCAATATACGTGGGGCCTCGTTCAAGAAGCATTTCCGGTGTGACTGAAAACGGCTACTATATCATCACACCTCTTATCCCGATCCCTGAAAAACCGGAGAGATCTTCCAGTGTGCAGCCACCAATTTTGGTCAACAGAGGATGGGTACCCCGAAGTTGGAAAGATGAGGTTTCAAAGGTTTCAAAAGATGGTGAACAACCTTCTGATATAAACCCCTCGTCTGTCCAAGAGACTGAAAGAAGGTCTTGGTGGAGGTTGTGGAGCAAGAAGCCCGAAGTTGTTGAGGATAAGACCCCTGCTGTTGCTCCTGTTGAAGTCGTTGGAGTAGTTCGAGGGAGTGAAAAGCCTAGCATATTTGTTCCACCAAATGATCCAAACTCCGGTCAATGGTTCTACGTTGATGTTCCTGCAATTGCTCGTACGTGTGGACTCCCTGAGGATACTGTTTACATTGACGACGCAAACGAAAATGTCAATCCAAGTAACCCTTACCCGCTTCCAAAGGATGTCAGTACCTTGATTCGGAGTTCAGTCATGCCTCAGGACCATCTGAATTATACTTTGACATGGTATTCCCTCTCTGCAGCAGTTACATTCATGGCTTTCATGAGACTAAAACCGAAAAAGAGCCGCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SURF1

Protein Analysis

352

Amino Acids

39.53

Weight (kDa)

9.56

Isoelectric Point (pI)

69.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SURF1 PF02104 74 - 334 7.1e-43 SURF1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 562
AccB1I GGYRCC 1 cut(s) 562
AciI CCGC 2 cut(s) 911, 1048
AclWI GGATC 3 cut(s) 372, 493, 782
AfaI GTAC 3 cut(s) 564, 838, 931
AfiI CCNNNNNNNGG 4 cut(s) 573, 797, 804, 939
AflIII ACRYGT 1 cut(s) 839
AgsI TTSAA 4 cut(s) 441, 591, 600, 731
AjnI CCWGG 2 cut(s) 178, 223
AluBI AGCT 4 cut(s) 40, 54, 117, 230
AluI AGCT 4 cut(s) 40, 54, 117, 230
Alw26I GTCTC 3 cut(s) 156, 641, 1021
AlwI GGATC 3 cut(s) 372, 493, 782
AoxI GGCC 3 cut(s) 84, 241, 431
ApeKI GCWGC 4 cut(s) 5, 143, 532, 1001
Asp700I GAANNNNTTC 1 cut(s) 448
Asp718I GGTACC 1 cut(s) 562
AspS9I GGNCC 4 cut(s) 57, 85, 431, 961
AsuHPI GGTGA 4 cut(s) 62, 150, 397, 620
AvaII GGWCC 2 cut(s) 57, 961
AxyI CCTNAGG 2 cut(s) 852, 957
BanI GGYRCC 1 cut(s) 562
BbvCI CCTCAGC 1 cut(s) 139
BbvI GCAGC 4 cut(s) 17, 155, 544, 1013
BccI CCATC 5 cut(s) 170, 201, 552, 599, 972
BceAI ACGGC 1 cut(s) 484
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 2 cut(s) 180, 225
BciVI GTATCC 1 cut(s) 850
BcoDI GTCTC 3 cut(s) 156, 641, 1021
BfaI CTAG 2 cut(s) 51, 765
BfmI CTRYAG 1 cut(s) 999
BfuI GTATCC 1 cut(s) 850
BglII AGATCT 1 cut(s) 518
BisI GCNGC 5 cut(s) 6, 144, 533, 1002, 1048
BlsI GCNGC 5 cut(s) 7, 145, 534, 1003, 1049
Bme1390I CCNGG 2 cut(s) 180, 225
Bme18I GGWCC 2 cut(s) 57, 961
BmgT120I GGNCC 4 cut(s) 57, 85, 431, 961
BmiI GGNNCC 5 cut(s) 59, 87, 321, 432, 564
BmrFI CCNGG 2 cut(s) 180, 225
BmsI GCATC 1 cut(s) 121
BpmI CTGGAG 3 cut(s) 97, 162, 246
Bpu10I CCTNAGC 1 cut(s) 139
BpuEI CTTGAG 1 cut(s) 352
BsaAI YACGTR 2 cut(s) 427, 840
BsaJI CCNNGG 2 cut(s) 179, 244
BsaWI WCCGGW 3 cut(s) 452, 511, 797
Bsc4I CCNNNNNNNGG 4 cut(s) 573, 797, 804, 939
Bse1I ACTGG 1 cut(s) 525
Bse21I CCTNAGG 2 cut(s) 852, 957
Bse3DI GCAATG 2 cut(s) 18, 343
BseBI CCWGG 2 cut(s) 180, 225
BseDI CCNNGG 2 cut(s) 179, 244
BseGI GGATG 2 cut(s) 563, 928
BseLI CCNNNNNNNGG 4 cut(s) 573, 797, 804, 939
BseMI GCAATG 2 cut(s) 18, 343
BseMII CTCAG 3 cut(s) 153, 843, 971
BseNI ACTGG 1 cut(s) 525
BseRI GAGGAG 1 cut(s) 109
BseXI GCAGC 4 cut(s) 17, 155, 544, 1013
BsgI GTGCAG 1 cut(s) 551
BshFI GGCC 3 cut(s) 86, 243, 433
BshNI GGYRCC 1 cut(s) 562
BsiSI CCGG 3 cut(s) 453, 512, 798
BsiWI CGTACG 1 cut(s) 836
BslFI GGGAC 2 cut(s) 43, 262
BslI CCNNNNNNNGG 4 cut(s) 573, 797, 804, 939
BsmAI GTCTC 3 cut(s) 156, 641, 1021
BsmFI GGGAC 2 cut(s) 43, 262
BsnI GGCC 3 cut(s) 86, 243, 433
Bsp143I GATC 4 cut(s) 364, 498, 518, 787
BspACI CCGC 2 cut(s) 911, 1048
BspANI GGCC 3 cut(s) 86, 243, 433
BspCNI CTCAG 3 cut(s) 152, 844, 970
BspHI TCATGA 1 cut(s) 1023
BspLI GGNNCC 5 cut(s) 59, 87, 321, 432, 564
BspMAI CTGCAG 1 cut(s) 1003
BspPI GGATC 3 cut(s) 372, 493, 782
BspT107I GGYRCC 1 cut(s) 562
BsrDI GCAATG 2 cut(s) 18, 343
BsrI ACTGG 1 cut(s) 525
BssECI CCNNGG 2 cut(s) 179, 244
BssMI GATC 4 cut(s) 364, 498, 518, 787
BssT1I CCWWGG 1 cut(s) 244
Bst2UI CCWGG 2 cut(s) 180, 225
Bst4CI ACNGT 1 cut(s) 862
Bst6I CTCTTC 3 cut(s) 106, 172, 299
BstBAI YACGTR 2 cut(s) 427, 840
BstDEI CTNAG 3 cut(s) 139, 852, 957
BstF5I GGATG 2 cut(s) 563, 928
BstKTI GATC 4 cut(s) 367, 501, 521, 790
BstMAI GTCTC 3 cut(s) 156, 641, 1021
BstMBI GATC 4 cut(s) 364, 498, 518, 787
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstNI CCWGG 2 cut(s) 180, 225
BstSCI CCNGG 2 cut(s) 178, 223
BstSFI CTRYAG 1 cut(s) 999
BstV1I GCAGC 4 cut(s) 17, 155, 544, 1013
BstX2I RGATCY 1 cut(s) 518
BstXI CCANNNNNNTGG 1 cut(s) 82
BstYI RGATCY 1 cut(s) 518
Bsu36I CCTNAGG 2 cut(s) 852, 957
BsuI GTATCC 1 cut(s) 850
BsuRI GGCC 3 cut(s) 86, 243, 433
BtsCI GGATG 2 cut(s) 563, 928
BtsIMutI CAGTG 1 cut(s) 532
CciI TCATGA 1 cut(s) 1023
Cfr13I GGNCC 4 cut(s) 57, 85, 431, 961
CseI GACGC 1 cut(s) 884
Csp6I GTAC 3 cut(s) 563, 837, 930
CspCI CAANNNNNGTGG 4 cut(s) 408, 443, 525, 560
CviAII CATG 4 cut(s) 952, 984, 1015, 1024
CviQI GTAC 3 cut(s) 563, 837, 930
DdeI CTNAG 3 cut(s) 139, 852, 957
DpnI GATC 4 cut(s) 366, 500, 520, 789
DpnII GATC 4 cut(s) 364, 498, 518, 787
DraI TTTAAA 1 cut(s) 327
Eam1104I CTCTTC 3 cut(s) 106, 172, 299
EarI CTCTTC 3 cut(s) 106, 172, 299
Eco130I CCWWGG 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 57, 961
Eco81I CCTNAGG 2 cut(s) 852, 957
EcoO109I RGGNCCY 1 cut(s) 431
EcoRII CCWGG 2 cut(s) 178, 223
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 4 cut(s) 955, 987, 1018, 1027
FalI AAGNNNNNCTT 4 cut(s) 646, 678, 889, 921
FaqI GGGAC 2 cut(s) 43, 262
FatI CATG 4 cut(s) 951, 983, 1014, 1023
FauI CCCGC 1 cut(s) 918
Fnu4HI GCNGC 5 cut(s) 6, 144, 533, 1002, 1048
FokI GGATG 2 cut(s) 570, 935
Fsp4HI GCNGC 5 cut(s) 6, 144, 533, 1002, 1048
FspBI CTAG 2 cut(s) 51, 765
GluI GCNGC 5 cut(s) 6, 144, 533, 1002, 1048
GsuI CTGGAG 3 cut(s) 97, 162, 246
HaeIII GGCC 3 cut(s) 86, 243, 433
HapII CCGG 3 cut(s) 453, 512, 798
HgaI GACGC 1 cut(s) 884
Hin1II CATG 4 cut(s) 955, 987, 1018, 1027
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 3 cut(s) 149, 846, 937
HpaII CCGG 3 cut(s) 453, 512, 798
HphI GGTGA 4 cut(s) 62, 150, 397, 620
Hpy166II GTNNAC 4 cut(s) 550, 611, 845, 865
Hpy188I TCNGA 4 cut(s) 266, 622, 942, 969
Hpy188III TCNNGA 7 cut(s) 195, 204, 356, 441, 496, 959, 1024
Hpy8I GTNNAC 4 cut(s) 550, 611, 845, 865
Hpy99I CGWCG 1 cut(s) 878
HpyAV CCTTC 5 cut(s) 118, 178, 261, 627, 651
HpyCH4III ACNGT 1 cut(s) 862
HpyCH4IV ACGT 3 cut(s) 426, 813, 839
HpyCH4V TGCA 4 cut(s) 393, 532, 827, 1001
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 3 cut(s) 139, 852, 957
HpySE526I ACGT 3 cut(s) 426, 813, 839
Hsp92II CATG 4 cut(s) 955, 987, 1018, 1027
KpnI GGTACC 1 cut(s) 566
Kzo9I GATC 4 cut(s) 364, 498, 518, 787
LmnI GCTCC 4 cut(s) 122, 227, 678, 727
Lsp1109I GCAGC 4 cut(s) 17, 155, 544, 1013
LweI GCATC 1 cut(s) 121
MaeI CTAG 2 cut(s) 51, 765
MaeII ACGT 3 cut(s) 426, 813, 839
MaeIII GTNAC 3 cut(s) 457, 899, 1006
MalI GATC 4 cut(s) 366, 500, 520, 789
MboI GATC 4 cut(s) 364, 498, 518, 787
MboII GAAGA 7 cut(s) 57, 93, 96, 159, 212, 316, 513
MfeI CAATTG 1 cut(s) 828
MflI RGATCY 1 cut(s) 518
MluCI AATT 5 cut(s) 314, 359, 540, 828, 970
MlyI GAGTC 2 cut(s) 158, 840
MmeI TCCRAC 4 cut(s) 291, 351, 554, 718
MroXI GAANNNNTTC 1 cut(s) 448
MseI TTAA 1 cut(s) 326
MspI CCGG 3 cut(s) 453, 512, 798
MspR9I CCNGG 2 cut(s) 180, 225
MunI CAATTG 1 cut(s) 828
MvaI CCWGG 2 cut(s) 180, 225
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 4 cut(s) 364, 498, 518, 787
NlaIII CATG 4 cut(s) 955, 987, 1018, 1027
NlaIV GGNNCC 5 cut(s) 59, 87, 321, 432, 564
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 1 cut(s) 457
PagI TCATGA 1 cut(s) 1023
PcsI WCGNNNNNNNCGW 2 cut(s) 200, 879
PdmI GAANNNNTTC 1 cut(s) 448
PfeI GAWTC 1 cut(s) 937
Pfl23II CGTACG 1 cut(s) 836
PfoI TCCNGGA 1 cut(s) 223
PkrI GCNGC 5 cut(s) 7, 145, 534, 1003, 1049
PleI GAGTC 2 cut(s) 157, 840
PpsI GAGTC 2 cut(s) 157, 840
Ppu21I YACGTR 2 cut(s) 427, 840
Psp6I CCWGG 2 cut(s) 178, 223
PspGI CCWGG 2 cut(s) 178, 223
PspLI CGTACG 1 cut(s) 836
PspN4I GGNNCC 5 cut(s) 59, 87, 321, 432, 564
PspPI GGNCC 4 cut(s) 57, 85, 431, 961
PstI CTGCAG 1 cut(s) 1003
PsuI RGATCY 1 cut(s) 518
RsaI GTAC 3 cut(s) 564, 838, 931
RsaNI GTAC 3 cut(s) 563, 837, 930
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 5 cut(s) 6, 144, 533, 1002, 1048
Sau3AI GATC 4 cut(s) 364, 498, 518, 787
Sau96I GGNCC 4 cut(s) 57, 85, 431, 961
SchI GAGTC 2 cut(s) 158, 840
ScrFI CCNGG 2 cut(s) 180, 225
SfaNI GCATC 1 cut(s) 121
SfcI CTRYAG 1 cut(s) 999
SinI GGWCC 2 cut(s) 57, 961
SmlI CTYRAG 1 cut(s) 331
SmoI CTYRAG 1 cut(s) 331
Sse9I AATT 5 cut(s) 314, 359, 540, 828, 970
SsiI CCGC 2 cut(s) 911, 1048
SspMI CTAG 2 cut(s) 51, 765
StyD4I CCNGG 2 cut(s) 178, 223
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 1 cut(s) 862
TaiI ACGT 3 cut(s) 429, 816, 842
TaqI TCGA 3 cut(s) 194, 203, 748
TasI AATT 5 cut(s) 314, 359, 540, 828, 970
TauI GCSGC 1 cut(s) 1050
TfiI GAWTC 1 cut(s) 937
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TscAI CASTG 1 cut(s) 532
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 4 cut(s) 5, 143, 532, 1001
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 2 cut(s) 1003, 1012
TspGWI ACGGA 2 cut(s) 316, 336
TspRI CASTG 1 cut(s) 532
VpaK11BI GGWCC 2 cut(s) 57, 961
XmnI GAANNNNTTC 1 cut(s) 448
XspI CTAG 2 cut(s) 51, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.