MD14G1237600.v1.1

Probably involved in the biogenesis of the COX complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009259
Physical Location & Seq
Reverse (-)
31716445 .. 31719571
3127 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1237600.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGGCAGCAAAAACCTCCATTGCCAAAACCATAACCAAGCTTTATTATTCGAGTGGGTTCCATTCCTCTCACCCCAAACATCTGGCCCCACTCTCACTATCTTCCTCCTTCTCCAGCTCCTCCCCTAATGCTGCTTCCCCAGCGGCCGAGTCTCAATCAACCATCTCTTCCCAAGCTCCTGGGAGGGAAAGGTCGAGATGGTCGAGATGGTTGCTGTTTCTTCCTGGAGCTATCACTTTTGGGCTTGGGACTTGGCAGATTATCAGAAGGCAAGAGAAGATACAAATGTTAGATTATATACAGAAGAGGTTGGAATTGGAACCTTTAAACTTGAACAATGCCTCTCCGTCAAGTCAAAATTTGGATCAGTTGGAGTTTAGGAGGGTCAAATGCAAAGGGTATTTTGATGAGAAAAGGTCGATCTATGTGGGCCCTCGTTCAAGAAGCATTTCTGGTGTGACCGAAAATGGCTACTATATCATCACACCTCTTATCCAGGTCCCTGACAAAGATGAGAGTGTGCAGCCACCAATTTTGGTCAACAGAGGATGGGTGCTGCGAAGTTGGAAAGATGAGGCTTCAAAAGTTTCAGAAGGTGGTGAACAATCTTCTGATATAACACCCACCTCTGTCCAAGATACTGAAAGAAGGTCTTGGTCGAGGTTCTGGACCAAGAAGCCCCAAGTTGTTGAGGATCAAAACCGCGTTGTTGCTCCCGTTGAAGTTGTTGGAGTAGTTCGAGGGAGTGAAAAGCCTAGCATATTTGTTCCACCAAATGATCCAAACTCCTACCAATGGTTCTATGTTGATGTTCCTGCAATAGCTCGTACGTGTGGACTCCCTGAGGATACTATTTACATTGAAGACGTAAATGAAAATGTTAATCCAGGTAACCCTTACCCGCTTCCAAAGGATGTCAATACCTTGATTCGGAGTTCAGTCATGCCTCAGGACCACCTGAATTATACTCTGACATGGTATCCACTCTCTGCAGCAGTTACACTCATGGCTCTCATGAGTCTAAAACCCAAAAGGAGCCGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SURF1

Protein Analysis

349

Amino Acids

39.22

Weight (kDa)

9.34

Isoelectric Point (pI)

68.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SURF1 PF02104 74 - 331 2.2e-41 SURF1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 705
AciI CCGC 3 cut(s) 143, 703, 902
AclWI GGATC 3 cut(s) 372, 702, 773
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 1 cut(s) 358
AfaI GTAC 1 cut(s) 829
AfiI CCNNNNNNNGG 2 cut(s) 795, 930
AflIII ACRYGT 1 cut(s) 830
AgsI TTSAA 5 cut(s) 334, 441, 582, 722, 863
AjnI CCWGG 4 cut(s) 178, 223, 495, 886
AluBI AGCT 5 cut(s) 40, 117, 176, 230, 824
AluI AGCT 5 cut(s) 40, 117, 176, 230, 824
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 3 cut(s) 372, 702, 773
AoxI GGCC 3 cut(s) 84, 144, 430
ApaI GGGCCC 1 cut(s) 434
ApeKI GCWGC 5 cut(s) 5, 131, 523, 556, 992
ApoI RAATTY 1 cut(s) 358
Asp700I GAANNNNTTC 1 cut(s) 448
AspS9I GGNCC 6 cut(s) 85, 430, 431, 499, 669, 952
AsuHPI GGTGA 2 cut(s) 62, 611
AvaII GGWCC 3 cut(s) 499, 669, 952
AxyI CCTNAGG 2 cut(s) 843, 948
BaeGI GKGCMC 1 cut(s) 434
BaeI ACNNNNGTAYC 2 cut(s) 272, 305
BanII GRGCYC 1 cut(s) 434
BbsI GAAGAC 1 cut(s) 870
BbvI GCAGC 5 cut(s) 17, 118, 535, 543, 1004
BccI CCATC 4 cut(s) 170, 192, 201, 543
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 4 cut(s) 180, 225, 497, 888
BciVI GTATCC 2 cut(s) 841, 990
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 1 cut(s) 756
BfmI CTRYAG 1 cut(s) 990
BfuI GTATCC 2 cut(s) 841, 990
BisI GCNGC 6 cut(s) 6, 132, 144, 524, 557, 993
BlsI GCNGC 6 cut(s) 7, 133, 145, 525, 558, 994
Bme1390I CCNGG 4 cut(s) 180, 225, 497, 888
Bme18I GGWCC 3 cut(s) 499, 669, 952
BmgT120I GGNCC 6 cut(s) 85, 430, 431, 499, 669, 952
BmiI GGNNCC 6 cut(s) 59, 87, 321, 432, 501, 1037
BmrFI CCNGG 4 cut(s) 180, 225, 497, 888
BpiI GAAGAC 1 cut(s) 870
BpmI CTGGAG 2 cut(s) 97, 246
BsaAI YACGTR 1 cut(s) 831
BsaJI CCNNGG 1 cut(s) 179
Bsc4I CCNNNNNNNGG 2 cut(s) 795, 930
Bse21I CCTNAGG 2 cut(s) 843, 948
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 4 cut(s) 180, 225, 497, 888
BseDI CCNNGG 1 cut(s) 179
BseGI GGATG 2 cut(s) 554, 919
BseLI CCNNNNNNNGG 2 cut(s) 795, 930
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 2 cut(s) 834, 962
BseRI GAGGAG 1 cut(s) 109
BseSI GKGCMC 1 cut(s) 434
BseX3I CGGCCG 1 cut(s) 144
BseXI GCAGC 5 cut(s) 17, 118, 535, 543, 1004
BseYI CCCAGC 1 cut(s) 139
BsgI GTGCAG 1 cut(s) 542
Bsh1236I CGCG 1 cut(s) 705
Bsh1285I CGRYCG 1 cut(s) 147
BshFI GGCC 3 cut(s) 86, 146, 432
BsiEI CGRYCG 1 cut(s) 147
BsiSI CCGG 1 cut(s) 1039
BsiWI CGTACG 1 cut(s) 827
BslFI GGGAC 2 cut(s) 262, 485
BslI CCNNNNNNNGG 2 cut(s) 795, 930
BsmAI GTCTC 1 cut(s) 156
BsmFI GGGAC 2 cut(s) 262, 485
BsnI GGCC 3 cut(s) 86, 146, 432
Bsp120I GGGCCC 1 cut(s) 430
Bsp1286I GDGCHC 1 cut(s) 434
Bsp143I GATC 4 cut(s) 364, 420, 694, 778
BspACI CCGC 3 cut(s) 143, 703, 902
BspANI GGCC 3 cut(s) 86, 146, 432
BspCNI CTCAG 2 cut(s) 835, 961
BspFNI CGCG 1 cut(s) 705
BspHI TCATGA 1 cut(s) 1014
BspLI GGNNCC 6 cut(s) 59, 87, 321, 432, 501, 1037
BspMAI CTGCAG 1 cut(s) 994
BspPI GGATC 3 cut(s) 372, 702, 773
BsrDI GCAATG 1 cut(s) 18
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 4 cut(s) 364, 420, 694, 778
Bst2UI CCWGG 4 cut(s) 180, 225, 497, 888
Bst6I CTCTTC 2 cut(s) 172, 299
BstBAI YACGTR 1 cut(s) 831
BstDEI CTNAG 2 cut(s) 843, 948
BstEII GGTNACC 1 cut(s) 890
BstF5I GGATG 2 cut(s) 554, 919
BstFNI CGCG 1 cut(s) 705
BstKTI GATC 4 cut(s) 367, 423, 697, 781
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 4 cut(s) 364, 420, 694, 778
BstMCI CGRYCG 1 cut(s) 147
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstNI CCWGG 4 cut(s) 180, 225, 497, 888
BstPI GGTNACC 1 cut(s) 890
BstSCI CCNGG 4 cut(s) 178, 223, 495, 886
BstSFI CTRYAG 1 cut(s) 990
BstSLI GKGCMC 1 cut(s) 434
BstUI CGCG 1 cut(s) 705
BstV1I GCAGC 5 cut(s) 17, 118, 535, 543, 1004
BstV2I GAAGAC 1 cut(s) 870
BstXI CCANNNNNNTGG 2 cut(s) 82, 179
BstZI CGGCCG 1 cut(s) 144
Bsu36I CCTNAGG 2 cut(s) 843, 948
BsuI GTATCC 2 cut(s) 841, 990
BsuRI GGCC 3 cut(s) 86, 146, 432
BtsCI GGATG 2 cut(s) 554, 919
CciI TCATGA 1 cut(s) 1014
Cfr13I GGNCC 6 cut(s) 85, 430, 431, 499, 669, 952
Csp6I GTAC 1 cut(s) 828
CspCI CAANNNNNGTGG 2 cut(s) 516, 551
CviAII CATG 4 cut(s) 943, 975, 1006, 1015
CviQI GTAC 1 cut(s) 828
DdeI CTNAG 2 cut(s) 843, 948
DpnI GATC 4 cut(s) 366, 422, 696, 780
DpnII GATC 4 cut(s) 364, 420, 694, 778
DraI TTTAAA 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 144
EagI CGGCCG 1 cut(s) 144
Eam1104I CTCTTC 2 cut(s) 172, 299
EarI CTCTTC 2 cut(s) 172, 299
EclXI CGGCCG 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 434
Eco47I GGWCC 3 cut(s) 499, 669, 952
Eco52I CGGCCG 1 cut(s) 144
Eco81I CCTNAGG 2 cut(s) 843, 948
Eco91I GGTNACC 1 cut(s) 890
EcoO109I RGGNCCY 2 cut(s) 431, 499
EcoO65I GGTNACC 1 cut(s) 890
EcoRII CCWGG 4 cut(s) 178, 223, 495, 886
EcoT38I GRGCYC 1 cut(s) 434
FaeI CATG 4 cut(s) 946, 978, 1009, 1018
FalI AAGNNNNNCTT 2 cut(s) 637, 669
FaqI GGGAC 2 cut(s) 262, 485
FatI CATG 4 cut(s) 942, 974, 1005, 1014
FauI CCCGC 1 cut(s) 909
Fnu4HI GCNGC 6 cut(s) 6, 132, 144, 524, 557, 993
FokI GGATG 2 cut(s) 561, 926
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 6 cut(s) 6, 132, 144, 524, 557, 993
FspBI CTAG 1 cut(s) 756
GluI GCNGC 6 cut(s) 6, 132, 144, 524, 557, 993
GsaI CCCAGC 1 cut(s) 143
GsuI CTGGAG 2 cut(s) 97, 246
HaeIII GGCC 3 cut(s) 86, 146, 432
HapII CCGG 1 cut(s) 1039
Hin1II CATG 4 cut(s) 946, 978, 1009, 1018
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 4 cut(s) 149, 837, 928, 1018
HpaII CCGG 1 cut(s) 1039
HphI GGTGA 2 cut(s) 62, 611
Hpy166II GTNNAC 3 cut(s) 541, 602, 836
Hpy188I TCNGA 5 cut(s) 266, 592, 613, 933, 972
Hpy188III TCNNGA 6 cut(s) 195, 204, 441, 667, 950, 1015
Hpy8I GTNNAC 3 cut(s) 541, 602, 836
HpyAV CCTTC 4 cut(s) 118, 261, 587, 642
HpyCH4IV ACGT 2 cut(s) 830, 867
HpyCH4V TGCA 4 cut(s) 393, 523, 818, 992
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 2 cut(s) 843, 948
HpySE526I ACGT 2 cut(s) 830, 867
Hsp92II CATG 4 cut(s) 946, 978, 1009, 1018
Kzo9I GATC 4 cut(s) 364, 420, 694, 778
LmnI GCTCC 5 cut(s) 122, 181, 227, 718, 1035
Lsp1109I GCAGC 5 cut(s) 17, 118, 535, 543, 1004
MaeI CTAG 1 cut(s) 756
MaeII ACGT 2 cut(s) 830, 867
MaeIII GTNAC 3 cut(s) 457, 890, 997
MalI GATC 4 cut(s) 366, 422, 696, 780
MboI GATC 4 cut(s) 364, 420, 694, 778
MboII GAAGA 7 cut(s) 93, 159, 212, 289, 316, 600, 875
MhlI GDGCHC 1 cut(s) 434
MluCI AATT 4 cut(s) 314, 358, 531, 961
MlyI GAGTC 3 cut(s) 158, 831, 1027
MmeI TCCRAC 4 cut(s) 291, 351, 545, 709
MroXI GAANNNNTTC 1 cut(s) 448
MseI TTAA 2 cut(s) 326, 882
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 1 cut(s) 1039
MspR9I CCNGG 4 cut(s) 180, 225, 497, 888
MvaI CCWGG 4 cut(s) 180, 225, 497, 888
MvnI CGCG 1 cut(s) 705
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 4 cut(s) 364, 420, 694, 778
NlaIII CATG 4 cut(s) 946, 978, 1009, 1018
NlaIV GGNNCC 6 cut(s) 59, 87, 321, 432, 501, 1037
NmeAIII GCCGAG 1 cut(s) 172
NmuCI GTSAC 1 cut(s) 457
PagI TCATGA 1 cut(s) 1014
PcsI WCGNNNNNNNCGW 1 cut(s) 200
PdmI GAANNNNTTC 1 cut(s) 448
PfeI GAWTC 1 cut(s) 928
Pfl23II CGTACG 1 cut(s) 827
PfoI TCCNGGA 1 cut(s) 223
PkrI GCNGC 6 cut(s) 7, 133, 145, 525, 558, 994
PleI GAGTC 3 cut(s) 157, 831, 1026
PpsI GAGTC 3 cut(s) 157, 831, 1026
Ppu21I YACGTR 1 cut(s) 831
PpuMI RGGWCCY 1 cut(s) 499
Psp5II RGGWCCY 1 cut(s) 499
Psp6I CCWGG 4 cut(s) 178, 223, 495, 886
PspEI GGTNACC 1 cut(s) 890
PspFI CCCAGC 1 cut(s) 139
PspGI CCWGG 4 cut(s) 178, 223, 495, 886
PspLI CGTACG 1 cut(s) 827
PspN4I GGNNCC 6 cut(s) 59, 87, 321, 432, 501, 1037
PspOMI GGGCCC 1 cut(s) 430
PspPI GGNCC 6 cut(s) 85, 430, 431, 499, 669, 952
PspPPI RGGWCCY 1 cut(s) 499
PstI CTGCAG 1 cut(s) 994
RsaI GTAC 1 cut(s) 829
RsaNI GTAC 1 cut(s) 828
SaqAI TTAA 2 cut(s) 326, 882
SatI GCNGC 6 cut(s) 6, 132, 144, 524, 557, 993
Sau3AI GATC 4 cut(s) 364, 420, 694, 778
Sau96I GGNCC 6 cut(s) 85, 430, 431, 499, 669, 952
SchI GAGTC 3 cut(s) 158, 831, 1027
ScrFI CCNGG 4 cut(s) 180, 225, 497, 888
SduI GDGCHC 1 cut(s) 434
SfcI CTRYAG 1 cut(s) 990
SinI GGWCC 3 cut(s) 499, 669, 952
Sse9I AATT 4 cut(s) 314, 358, 531, 961
SsiI CCGC 3 cut(s) 143, 703, 902
SspMI CTAG 1 cut(s) 756
StyD4I CCNGG 4 cut(s) 178, 223, 495, 886
TaiI ACGT 2 cut(s) 833, 870
TaqI TCGA 6 cut(s) 50, 194, 203, 419, 659, 739
TaqII GACCGA 1 cut(s) 476
TasI AATT 4 cut(s) 314, 358, 531, 961
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 1 cut(s) 928
Tru1I TTAA 2 cut(s) 326, 882
Tru9I TTAA 2 cut(s) 326, 882
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 5 cut(s) 5, 131, 523, 556, 992
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 1 cut(s) 888
TspGWI ACGGA 1 cut(s) 336
VpaK11BI GGWCC 3 cut(s) 499, 669, 952
XapI RAATTY 1 cut(s) 358
XmnI GAANNNNTTC 1 cut(s) 448
XspI CTAG 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.