MD06G1234600.v1.1

Protein SHOOT GRAVITROPISM 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006002
Physical Location & Seq
Reverse (-)
36550721 .. 36553992
3272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1234600.v1.1.491

Sequence Viewer

Length: 1512 bp
ATGTTAGCCAATAACTCTTCTTCCTCAGTCCCTTCTTCTGATCCCTTTTCTTGCTTAGAAAATGGCCACACCAATAAAAGAAAGAGAAGACCTGCAGGCACTCCAGATCCAGATGCGGAGGTGGTGTCACTTTCGCCGAAAACCTTGCTGGAATCGGATCGTTACATTTGCGAGATCTGCAACCAAGGGTTTCAGAGAGACCAGAACCTACAGATGCACCGGCGGCGGCACAAGGTGCCGTGGAAGCTGCTGAAGCGAGAGAGTCCGGTCGTGAAGAAGCGGGTTTTTGTGTGCCCGGAACCAAGCTGCCTGCACCATGATCCCTGCCACGCCCTCGGCGATCTCGTCGGCATAAAAAAGCACTTCAGGAGAAAGCACAGTAACCACAAACAGTGGGTTTGTGATAAGTGCTCAAAAGGGTATGCAGTTCAATCAGATTACAAGGCACATCTCAAAACCTGCGGTACCAGAGGACATTCTTGTGACTGCGGCCGTGTTTTCTCTAGGGTTGAGAGTTTCATAGAGCACCAAGATGCTTGCAACATGGATCGTGTGCGGCCGGAATCACAAACGCTTCAACCGGGGGCATGCCTGTCACGAACGGCTTCAAGTCCTAGTCCATCTTGCAGTGACAACAATTTTAGCAGGGCTCCTTCCACTTGGCCTCCATCTAATAATTCGGTACTACCACATCCAAGTCCAACCGAGTACACCAAATTAACCTTAAAAAACCCTCCCGCTCACGCTGATCATCATCAAGCTACACTGTCCAAGAAGAATAGCAGTAATGCACATTACAATTTGGATCTTCAGCTCGCAACCACATCGAATATTAATATTCCGATCAACGAAGTCTCTGTTTCGTCTAAGAGAGAAGAAAACCATTCCACTCAGCTGCAGCTCTCAATTGGTTCATGCGAATTTGGCGGGGGTGGTCATAGTCATCAGAACAAAAATATTGAACAACATTCAAACATTATGATCGCGAATCAAAATTACTCCCCGAGGGAGAACGAGATGAAGGAAAGGGAAAGGGAAAGCAGCAGCACTACTACTAATCATCACGAGCGTGATGACGATGATCAGAAACCTAAACTTGCCGCCACGGCTGCTGGGGTATTGAGGCTGAAAGAAGAAGCGCGAGAGCAGTTAAGGCTTGCGATGGCGGAAAAGGCTTACGCCGAAGAGGCGAGGCAGCAAGCCGGGCGCCACATCGAACTGGCGGAGAAGGAGTTTGCAAATGCCAAGAGAATAAGGCAACAAGCGCAGGCCGAGTTGGACAAGGCTCACGCTTTGAAAGAGCAAGCCATCAAGAAAGTGAACTCCACCATTCTCCAAATCACTTGCCATGCGTGCAATAAACAGTTTATTCAAGGCGCTCATGAAGCAAAAACCAATCAACATTGTCTGGTTTTGAGTTACATGTCCTCGGCTATAGCATTTGAAGGTGATCATCTATTAGAGAAGAATATTAGTACTCGAACCCATCATGCAAAATCTGCCAACGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C2H2

Protein Analysis

504

Amino Acids

56.38

Weight (kDa)

8.94

Isoelectric Point (pI)

68.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-2nd_BIRD-IDD PF22996 92 - 126 4.9e-18 BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD PF22995 131 - 155 4.4e-14 BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD PF22992 159 - 181 2.8e-06 BIRD-IDD transcription factor, fourth C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 100, 467
Acc65I GGTACC 1 cut(s) 464
AccB1I GGYRCC 3 cut(s) 235, 464, 1206
AccBSI CCGCTC 1 cut(s) 740
AccII CGCG 2 cut(s) 986, 1141
AclWI GGATC 6 cut(s) 35, 101, 165, 314, 555, 813
AcoI YGGCCR 3 cut(s) 64, 490, 557
AcsI RAATTY 1 cut(s) 920
AcuI CTGAAG 3 cut(s) 272, 349, 794
AcyI GRCGYC 1 cut(s) 1207
AdeI CACNNNGTG 1 cut(s) 235
AfaI GTAC 4 cut(s) 466, 684, 710, 1477
AflIII ACRYGT 1 cut(s) 1422
AgsI TTSAA 8 cut(s) 431, 578, 609, 962, 972, 1297, 1373, 1445
AleI CACNNNNGTG 1 cut(s) 1068
AluBI AGCT 6 cut(s) 247, 306, 761, 814, 895, 901
AluI AGCT 6 cut(s) 247, 306, 761, 814, 895, 901
Alw21I GWGCWC 2 cut(s) 413, 528
Alw26I GTCTC 2 cut(s) 192, 859
AlwI GGATC 6 cut(s) 35, 101, 165, 314, 555, 813
Ama87I CYCGRG 1 cut(s) 1003
AoxI GGCC 5 cut(s) 64, 490, 557, 662, 1269
ApeKI GCWGC 8 cut(s) 247, 306, 895, 898, 1041, 1044, 1109, 1195
ApoI RAATTY 1 cut(s) 920
AseI ATTAAT 1 cut(s) 834
Asp700I GAANNNNTTC 1 cut(s) 604
Asp718I GGTACC 1 cut(s) 464
AspLEI GCGC 4 cut(s) 1141, 1209, 1267, 1379
AsuC2I CCSGG 3 cut(s) 296, 582, 1204
AsuHPI GGTGA 1 cut(s) 1460
AvaI CYCGRG 1 cut(s) 1003
BaeGI GKGCMC 1 cut(s) 296
BalI TGGCCA 1 cut(s) 66
BanI GGYRCC 3 cut(s) 235, 464, 1206
BanII GRGCYC 1 cut(s) 652
BauI CACGAG 1 cut(s) 1064
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 2 cut(s) 413, 528
BbvI GCAGC 8 cut(s) 234, 293, 882, 910, 1053, 1056, 1096, 1207
BccI CCATC 5 cut(s) 628, 676, 1156, 1316, 1494
BceAI ACGGC 4 cut(s) 223, 477, 618, 1122
BcgI CGANNNNNNTGC 4 cut(s) 127, 161, 807, 841
BclI TGATCA 3 cut(s) 748, 1081, 1450
BcnI CCSGG 3 cut(s) 296, 582, 1204
BcoDI GTCTC 2 cut(s) 192, 859
BfaI CTAG 2 cut(s) 504, 615
BfmI CTRYAG 4 cut(s) 93, 209, 896, 1434
BfoI RGCGCY 2 cut(s) 1210, 1380
BfuAI ACCTGC 2 cut(s) 100, 467
BglI GCCNNNNNGGC 2 cut(s) 1106, 1187
BglII AGATCT 1 cut(s) 174
BmcAI AGTACT 1 cut(s) 1477
Bme1390I CCNGG 3 cut(s) 296, 582, 1204
BmeT110I CYCGRG 1 cut(s) 1003
BmiI GGNNCC 5 cut(s) 237, 300, 466, 651, 1208
BmrFI CCNGG 3 cut(s) 296, 582, 1204
BmsI GCATC 3 cut(s) 103, 204, 523
BpiI GAAGAC 1 cut(s) 94
BpmI CTGGAG 1 cut(s) 87
BpuMI CCSGG 3 cut(s) 296, 582, 1204
BsaBI GATNNNNATC 1 cut(s) 753
BsaHI GRCGYC 1 cut(s) 1207
BsaI GGTCTC 1 cut(s) 192
BsaJI CCNNGG 7 cut(s) 184, 239, 334, 581, 1004, 1104, 1428
BsaWI WCCGGW 1 cut(s) 265
BsaXI ACNNNNNCTCC 6 cut(s) 110, 140, 649, 679, 1217, 1247
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 1 cut(s) 1224
Bse8I GATNNNNATC 1 cut(s) 753
BseDI CCNNGG 7 cut(s) 184, 239, 334, 581, 1004, 1104, 1428
BseGI GGATG 1 cut(s) 691
BseJI GATNNNNATC 1 cut(s) 753
BseMII CTCAG 2 cut(s) 39, 905
BseNI ACTGG 1 cut(s) 1224
BseSI GKGCMC 1 cut(s) 296
BseX3I CGGCCG 2 cut(s) 490, 557
BseXI GCAGC 8 cut(s) 234, 293, 882, 910, 1053, 1056, 1096, 1207
BseYI CCCAGC 1 cut(s) 1112
BsgI GTGCAG 1 cut(s) 296
Bsh1236I CGCG 2 cut(s) 986, 1141
Bsh1285I CGRYCG 3 cut(s) 270, 493, 560
BshFI GGCC 5 cut(s) 66, 492, 559, 664, 1271
BshNI GGYRCC 3 cut(s) 235, 464, 1206
BsiEI CGRYCG 3 cut(s) 270, 493, 560
BsiHKAI GWGCWC 2 cut(s) 413, 528
BsiHKCI CYCGRG 1 cut(s) 1003
BsiSI CCGG 6 cut(s) 220, 266, 296, 560, 581, 1203
BslFI GGGAC 1 cut(s) 14
BsmAI GTCTC 2 cut(s) 192, 859
BsmFI GGGAC 1 cut(s) 14
BsnI GGCC 5 cut(s) 66, 492, 559, 664, 1271
Bso31I GGTCTC 1 cut(s) 192
BsoBI CYCGRG 1 cut(s) 1003
Bsp1286I GDGCHC 4 cut(s) 296, 413, 528, 652
Bsp68I TCGCGA 1 cut(s) 986
BspANI GGCC 5 cut(s) 66, 492, 559, 664, 1271
BspCNI CTCAG 2 cut(s) 38, 904
BspFNI CGCG 2 cut(s) 986, 1141
BspHI TCATGA 1 cut(s) 1381
BspLI GGNNCC 5 cut(s) 237, 300, 466, 651, 1208
BspMAI CTGCAG 2 cut(s) 97, 900
BspMI ACCTGC 2 cut(s) 100, 467
BspPI GGATC 6 cut(s) 35, 101, 165, 314, 555, 813
BspT107I GGYRCC 3 cut(s) 235, 464, 1206
BspTNI GGTCTC 1 cut(s) 192
BsrBI CCGCTC 1 cut(s) 740
BsrFI RCCGGY 1 cut(s) 219
BsrI ACTGG 1 cut(s) 1224
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 7 cut(s) 184, 239, 334, 581, 1004, 1104, 1428
BssNI GRCGYC 1 cut(s) 1207
BssSI CACGAG 1 cut(s) 1064
BssT1I CCWWGG 1 cut(s) 184
Bst2BI CACGAG 1 cut(s) 1064
Bst4CI ACNGT 4 cut(s) 380, 393, 768, 1365
Bst6I CTCTTC 2 cut(s) 22, 1179
BstACI GRCGYC 1 cut(s) 1207
BstAPI GCANNNNNTGC 2 cut(s) 235, 1499
BstDEI CTNAG 4 cut(s) 25, 55, 867, 891
BstDSI CCRYGG 2 cut(s) 239, 1104
BstF5I GGATG 1 cut(s) 691
BstFNI CGCG 2 cut(s) 986, 1141
BstH2I RGCGCY 2 cut(s) 1210, 1380
BstHHI GCGC 4 cut(s) 1141, 1209, 1267, 1379
BstMAI GTCTC 2 cut(s) 192, 859
BstMCI CGRYCG 3 cut(s) 270, 493, 560
BstNSI RCATGY 2 cut(s) 591, 1426
BstSCI CCNGG 3 cut(s) 294, 580, 1202
BstSFI CTRYAG 4 cut(s) 93, 209, 896, 1434
BstSLI GKGCMC 1 cut(s) 296
BstUI CGCG 2 cut(s) 986, 1141
BstV1I GCAGC 8 cut(s) 234, 293, 882, 910, 1053, 1056, 1096, 1207
BstV2I GAAGAC 1 cut(s) 94
BstX2I RGATCY 3 cut(s) 106, 174, 805
BstYI RGATCY 3 cut(s) 106, 174, 805
BstZI CGGCCG 2 cut(s) 490, 557
BsuRI GGCC 5 cut(s) 66, 492, 559, 664, 1271
BtgI CCRYGG 2 cut(s) 239, 1104
BtgZI GCGATG 1 cut(s) 1175
BtsCI GGATG 1 cut(s) 691
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 3 cut(s) 398, 634, 764
BtuMI TCGCGA 1 cut(s) 986
BveI ACCTGC 2 cut(s) 100, 467
CciI TCATGA 1 cut(s) 1381
CfoI GCGC 4 cut(s) 1141, 1209, 1267, 1379
Cfr10I RCCGGY 1 cut(s) 219
Csp6I GTAC 4 cut(s) 465, 683, 709, 1476
CviAII CATG 8 cut(s) 317, 544, 588, 915, 1349, 1382, 1423, 1490
CviQI GTAC 4 cut(s) 465, 683, 709, 1476
DdeI CTNAG 4 cut(s) 25, 55, 867, 891
DinI GGCGCC 1 cut(s) 1208
DraIII CACNNNGTG 1 cut(s) 235
EaeI YGGCCR 3 cut(s) 64, 490, 557
EagI CGGCCG 2 cut(s) 490, 557
Eam1104I CTCTTC 2 cut(s) 22, 1179
EarI CTCTTC 2 cut(s) 22, 1179
EciI GGCGGA 2 cut(s) 1181, 1238
EclXI CGGCCG 2 cut(s) 490, 557
Eco130I CCWWGG 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 652
Eco31I GGTCTC 1 cut(s) 192
Eco52I CGGCCG 2 cut(s) 490, 557
Eco57I CTGAAG 3 cut(s) 272, 349, 794
Eco88I CYCGRG 1 cut(s) 1003
EcoT14I CCWWGG 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 652
EgeI GGCGCC 1 cut(s) 1208
EheI GGCGCC 1 cut(s) 1208
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 8 cut(s) 320, 547, 591, 918, 1352, 1385, 1426, 1493
FaqI GGGAC 1 cut(s) 14
FatI CATG 8 cut(s) 316, 543, 587, 914, 1348, 1381, 1422, 1489
FauI CCCGC 3 cut(s) 273, 745, 920
FbaI TGATCA 3 cut(s) 748, 1081, 1450
FokI GGATG 1 cut(s) 678
FriOI GRGCYC 1 cut(s) 652
FspBI CTAG 2 cut(s) 504, 615
GlaI GCGC 4 cut(s) 1140, 1208, 1266, 1378
GsaI CCCAGC 1 cut(s) 1116
GsuI CTGGAG 1 cut(s) 87
HaeII RGCGCY 2 cut(s) 1210, 1380
HaeIII GGCC 5 cut(s) 66, 492, 559, 664, 1271
HapII CCGG 6 cut(s) 220, 266, 296, 560, 581, 1203
HhaI GCGC 4 cut(s) 1141, 1209, 1267, 1379
Hin1I GRCGYC 1 cut(s) 1207
Hin1II CATG 8 cut(s) 320, 547, 591, 918, 1352, 1385, 1426, 1493
Hin6I GCGC 4 cut(s) 1139, 1207, 1265, 1377
HinP1I GCGC 4 cut(s) 1139, 1207, 1265, 1377
HinfI GANTC 4 cut(s) 152, 262, 563, 988
HpaII CCGG 6 cut(s) 220, 266, 296, 560, 581, 1203
HphI GGTGA 1 cut(s) 1460
Hpy166II GTNNAC 2 cut(s) 711, 1321
Hpy188I TCNGA 7 cut(s) 40, 157, 195, 436, 843, 948, 1086
Hpy188III TCNNGA 9 cut(s) 104, 110, 271, 367, 597, 985, 1064, 1312, 1382
Hpy8I GTNNAC 2 cut(s) 711, 1321
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 5 cut(s) 42, 663, 1015, 1222, 1439
HpyCH4III ACNGT 4 cut(s) 380, 393, 768, 1365
HpyF3I CTNAG 4 cut(s) 25, 55, 867, 891
Hsp92I GRCGYC 1 cut(s) 1207
Hsp92II CATG 8 cut(s) 320, 547, 591, 918, 1352, 1385, 1426, 1493
HspAI GCGC 4 cut(s) 1139, 1207, 1265, 1377
KasI GGCGCC 1 cut(s) 1206
KpnI GGTACC 1 cut(s) 468
Ksp22I TGATCA 3 cut(s) 748, 1081, 1450
LmnI GCTCC 1 cut(s) 655
Lsp1109I GCAGC 8 cut(s) 234, 293, 882, 910, 1053, 1056, 1096, 1207
LweI GCATC 3 cut(s) 103, 204, 523
MaeI CTAG 2 cut(s) 504, 615
MaeIII GTNAC 7 cut(s) 126, 161, 380, 482, 594, 629, 1418
MbiI CCGCTC 1 cut(s) 740
MfeI CAATTG 1 cut(s) 906
MflI RGATCY 3 cut(s) 106, 174, 805
MhlI GDGCHC 4 cut(s) 296, 413, 528, 652
MlsI TGGCCA 1 cut(s) 66
MluCI AATT 7 cut(s) 637, 676, 716, 799, 906, 920, 994
MluNI TGGCCA 1 cut(s) 66
Mly113I GGCGCC 1 cut(s) 1207
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 2 cut(s) 725, 1257
Mox20I TGGCCA 1 cut(s) 66
MroXI GAANNNNTTC 1 cut(s) 604
MscI TGGCCA 1 cut(s) 66
MseI TTAA 4 cut(s) 719, 725, 834, 1151
MslI CAYNNNNRTG 3 cut(s) 480, 531, 1068
Msp20I TGGCCA 1 cut(s) 66
MspA1I CMGCKG 1 cut(s) 895
MspI CCGG 6 cut(s) 220, 266, 296, 560, 581, 1203
MspR9I CCNGG 3 cut(s) 296, 582, 1204
MunI CAATTG 1 cut(s) 906
MvnI CGCG 2 cut(s) 986, 1141
NarI GGCGCC 1 cut(s) 1207
NciI CCSGG 3 cut(s) 296, 582, 1204
NlaIII CATG 8 cut(s) 320, 547, 591, 918, 1352, 1385, 1426, 1493
NlaIV GGNNCC 5 cut(s) 237, 300, 466, 651, 1208
NmeAIII GCCGAG 3 cut(s) 315, 1297, 1409
NmuCI GTSAC 4 cut(s) 126, 482, 594, 629
NruI TCGCGA 1 cut(s) 986
NspI RCATGY 2 cut(s) 591, 1426
OliI CACNNNNGTG 1 cut(s) 1068
PaeI GCATGC 1 cut(s) 591
PagI TCATGA 1 cut(s) 1381
PciI ACATGT 1 cut(s) 1422
PcsI WCGNNNNNNNCGW 2 cut(s) 336, 342
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 3 cut(s) 152, 563, 988
PleI GAGTC 1 cut(s) 270
PluTI GGCGCC 1 cut(s) 1210
PpsI GAGTC 1 cut(s) 270
PscI ACATGT 1 cut(s) 1422
PshBI ATTAAT 1 cut(s) 834
PspFI CCCAGC 1 cut(s) 1112
PspN4I GGNNCC 5 cut(s) 237, 300, 466, 651, 1208
PstI CTGCAG 2 cut(s) 97, 900
PsuI RGATCY 3 cut(s) 106, 174, 805
PvuII CAGCTG 1 cut(s) 895
RruI TCGCGA 1 cut(s) 986
RsaI GTAC 4 cut(s) 466, 684, 710, 1477
RsaNI GTAC 4 cut(s) 465, 683, 709, 1476
RseI CAYNNNNRTG 3 cut(s) 480, 531, 1068
SaqAI TTAA 4 cut(s) 719, 725, 834, 1151
SbfI CCTGCAGG 1 cut(s) 97
ScaI AGTACT 1 cut(s) 1477
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 3 cut(s) 296, 582, 1204
SdaI CCTGCAGG 1 cut(s) 97
SduI GDGCHC 4 cut(s) 296, 413, 528, 652
SfaNI GCATC 3 cut(s) 103, 204, 523
SfcI CTRYAG 4 cut(s) 93, 209, 896, 1434
SfoI GGCGCC 1 cut(s) 1208
SgrAI CRCCGGYG 1 cut(s) 219
SmiMI CAYNNNNRTG 3 cut(s) 480, 531, 1068
SphI GCATGC 1 cut(s) 591
Sse8387I CCTGCAGG 1 cut(s) 97
Sse9I AATT 7 cut(s) 637, 676, 716, 799, 906, 920, 994
SspDI GGCGCC 1 cut(s) 1206
SspI AATATT 4 cut(s) 832, 838, 958, 1471
SspMI CTAG 2 cut(s) 504, 615
StyD4I CCNGG 3 cut(s) 294, 580, 1202
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 4 cut(s) 380, 393, 768, 1365
TaqI TCGA 3 cut(s) 827, 1215, 1480
TasI AATT 7 cut(s) 637, 676, 716, 799, 906, 920, 994
TatI WGTACW 2 cut(s) 708, 1475
TauI GCSGC 5 cut(s) 226, 229, 492, 559, 1103
TfiI GAWTC 3 cut(s) 152, 563, 988
Tru1I TTAA 4 cut(s) 719, 725, 834, 1151
Tru9I TTAA 4 cut(s) 719, 725, 834, 1151
TscAI CASTG 3 cut(s) 398, 634, 771
TseFI GTSAC 4 cut(s) 126, 482, 594, 629
TseI GCWGC 8 cut(s) 247, 306, 895, 898, 1041, 1044, 1109, 1195
Tsp45I GTSAC 4 cut(s) 126, 482, 594, 629
TspDTI ATGAA 4 cut(s) 508, 903, 1034, 1398
TspRI CASTG 3 cut(s) 398, 634, 771
VspI ATTAAT 1 cut(s) 834
XapI RAATTY 1 cut(s) 920
XceI RCATGY 2 cut(s) 591, 1426
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 2 cut(s) 504, 615
ZrmI AGTACT 1 cut(s) 1477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.