Rh7AG008500

Protein SHOOT GRAVITROPISM 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0006002
Physical Location & Seq
Forward (+)
573155 .. 576381
3227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG008500.1

Sequence Viewer

Length: 1269 bp
ATGCACCGGAGACGTCACAAGGTGCCCTGGAAGCTTCTGAAGAGAGAGACTCCGGTGGTGAGGAAACGGGTATTCGTGTGCCCCGAACCGAGTTGCCTGCACCACGACCCGTGCCACGCTCTGGGTGATCTGGTTGGGATAAAAAAGCACTTCAGAAGGAAGCACAGCAACCACAAGCAATGGGTTTGTGAGAAATGCTCGAAAGGCTACGCAGTTCAATCTGACTACAAAGCCCATCTCAAGACCTGCGGCACCCGAGGCCATTCTTGCGACTGTGGCCGTGTTTTCTCAAGGGTTGAGAGTTTCATTGAACACCAAGATGCTTGCAACATGGATCGTCTACGGCCGGAATCCCAAGCAGTAGTACTACAACCACCGGCATGCCTATCAAGAACGGCGTCAAGTCCAAGTCCGTCAAGCGAAACCAATTTCAGCAGCAGGACTACTCCTAACTGGCCTTCGGCTTCAGCTTTGGTGGTGCCAAAGCCGGCTCATGACAACACCAAGTTGATCTTAAGTAGTCCTCCTGCTGGCCACGGTATTAGTGACCGTAATCGAAACTCATCCAAGGATATCAATGCAAACAGCTTGTACAATTTGGATCTTCAGCTTGCGACCACGTCAAATGACAACAATCCTAACCATATTGAAGTCTCGGTTTCCTCGTCTAAGAGAGACGTGGAAAACCATTCCACTCAGCTCCAGCTCTCAATTGGATCGTGTGACAATTTTGGCGATCATCATCATCCAAAGAATATTAATATCGAATCAAATAGAAACTCTCCGAGGGAAAGCAGCACTAGTACTAATGAACATCACCACCAACAGATGAAACCAGCAGGTGAATCAGCATCAACATCAAGGATAATTAAAGAAGAAGCACGAGAGCAGTTAAGGCTTGCCTTGGCGGAAAAGGCTTACGCAGAAGAGGCGAGGCAGCAAGCGGAGCAGCAGATTGAGCTAGCCGAGCAGGAGTTTGCGAATGCCAAGAGAATAAGGCAGCAAGCACAAGCTGAATTGGACAAGGCGCAAGCTTTGAAAGAACATGCCGTTAAGAAAATCAACTCTACCATTCTCCAAATCACCTGTCAGGCTTGCAAGCAACAATTTCAAGCTAGAGCGAGGCAAGCAAACCCTCATGAGAACTCTCTGGCGTTCAGTTACATGTCATCGGCTTTAACAACAACAACAACTGAAGGTGACCAGTTAGAGATCAATGCTAGTGCTACTCGAACGGATGATCGGGCAAATTCATCTGCTAAACCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C2H2

Protein Analysis

422

Amino Acids

47.16

Weight (kDa)

9.04

Isoelectric Point (pI)

67.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-2nd_BIRD-IDD PF22996 21 - 55 1.4e-18 BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD PF22995 60 - 84 1.8e-14 BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD PF22992 88 - 110 1.9e-06 BIRD-IDD transcription factor, fourth C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 830
AatII GACGTC 1 cut(s) 16
Acc36I ACCTGC 2 cut(s) 254, 830
AccB1I GGYRCC 3 cut(s) 22, 251, 478
AccB7I CCANNNNNTGG 1 cut(s) 121
AccI GTMKAC 1 cut(s) 340
AciI CCGC 3 cut(s) 249, 908, 944
AclWI GGATC 3 cut(s) 342, 609, 724
AcoI YGGCCR 3 cut(s) 277, 344, 532
AcsI RAATTY 1 cut(s) 1249
AcuI CTGAAG 5 cut(s) 59, 136, 450, 590, 1215
AcyI GRCGYC 2 cut(s) 13, 398
AdeI CACNNNGTG 1 cut(s) 22
AfaI GTAC 3 cut(s) 366, 593, 805
AfiI CCNNNNNNNGG 2 cut(s) 121, 530
AflII CTTAAG 1 cut(s) 514
AflIII ACRYGT 1 cut(s) 1164
AgsI TTSAA 5 cut(s) 218, 311, 650, 1039, 1112
AhlI ACTAGT 1 cut(s) 800
AjiI CACGTC 2 cut(s) 621, 679
AjnI CCWGG 1 cut(s) 26
Alw26I GTCTC 4 cut(s) 4, 41, 658, 669
AlwI GGATC 3 cut(s) 342, 609, 724
Ama87I CYCGRG 1 cut(s) 255
AoxI GGCC 5 cut(s) 259, 277, 344, 455, 532
ApeKI GCWGC 5 cut(s) 435, 795, 937, 949, 1000
ApoI RAATTY 1 cut(s) 1249
ArsI GACNNNNNNTTYG 2 cut(s) 1072, 1104
AseI ATTAAT 1 cut(s) 759
AspLEI GCGC 1 cut(s) 1030
AsuHPI GGTGA 6 cut(s) 70, 137, 809, 854, 1075, 1211
AsuNHI GCTAGC 1 cut(s) 961
AvaI CYCGRG 1 cut(s) 255
BaeGI GKGCMC 2 cut(s) 27, 83
BalI TGGCCA 1 cut(s) 534
BanI GGYRCC 3 cut(s) 22, 251, 478
BauI CACGAG 1 cut(s) 882
BbvI GCAGC 5 cut(s) 447, 807, 949, 961, 1012
BccI CCATC 1 cut(s) 243
BceAI ACGGC 4 cut(s) 264, 359, 411, 1034
BcgI CGANNNNNNTGC 2 cut(s) 79, 113
BciT130I CCWGG 1 cut(s) 28
BcoDI GTCTC 4 cut(s) 4, 41, 658, 669
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 4 cut(s) 801, 962, 1116, 1221
BfrI CTTAAG 1 cut(s) 514
BfuAI ACCTGC 2 cut(s) 254, 830
BisI GCNGC 6 cut(s) 250, 436, 796, 938, 950, 1001
BlsI GCNGC 6 cut(s) 251, 437, 797, 939, 951, 1002
BmcAI AGTACT 2 cut(s) 366, 805
Bme1390I CCNGG 1 cut(s) 28
BmeT110I CYCGRG 1 cut(s) 255
BmgBI CACGTC 2 cut(s) 621, 679
BmiI GGNNCC 3 cut(s) 24, 253, 480
BmrFI CCNGG 1 cut(s) 28
BmsI GCATC 2 cut(s) 310, 860
BmtI GCTAGC 1 cut(s) 965
BplI GAGNNNNNCTC 4 cut(s) 34, 66, 182, 214
BpmI CTGGAG 1 cut(s) 686
BpuEI CTTGAG 2 cut(s) 224, 274
BsaBI GATNNNNATC 1 cut(s) 741
BsaHI GRCGYC 2 cut(s) 13, 398
BsaJI CCNNGG 6 cut(s) 26, 256, 535, 567, 785, 903
BsaWI WCCGGW 2 cut(s) 6, 52
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 530
Bse118I RCCGGY 2 cut(s) 376, 487
Bse1I ACTGG 2 cut(s) 458, 1204
Bse3DI GCAATG 1 cut(s) 185
Bse8I GATNNNNATC 1 cut(s) 741
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 6 cut(s) 26, 256, 535, 567, 785, 903
BseGI GGATG 3 cut(s) 563, 745, 1243
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 2 cut(s) 121, 530
BseMI GCAATG 1 cut(s) 185
BseMII CTCAG 1 cut(s) 710
BseNI ACTGG 2 cut(s) 458, 1204
BseSI GKGCMC 2 cut(s) 27, 83
BseX3I CGGCCG 1 cut(s) 344
BseXI GCAGC 5 cut(s) 447, 807, 949, 961, 1012
BsgI GTGCAG 1 cut(s) 83
Bsh1285I CGRYCG 1 cut(s) 347
BshFI GGCC 5 cut(s) 261, 279, 346, 457, 534
BshNI GGYRCC 3 cut(s) 22, 251, 478
BsiEI CGRYCG 1 cut(s) 347
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 5 cut(s) 7, 53, 347, 377, 488
BslI CCNNNNNNNGG 2 cut(s) 121, 530
BsmAI GTCTC 4 cut(s) 4, 41, 658, 669
BsmBI CGTCTC 2 cut(s) 4, 669
BsmI GAATGC 1 cut(s) 988
BsnI GGCC 5 cut(s) 261, 279, 346, 457, 534
BsoBI CYCGRG 1 cut(s) 255
Bsp1286I GDGCHC 2 cut(s) 27, 83
Bsp1407I TGTACA 1 cut(s) 591
Bsp143I GATC 8 cut(s) 127, 334, 510, 601, 716, 736, 1212, 1240
BspACI CCGC 3 cut(s) 249, 908, 944
BspANI GGCC 5 cut(s) 261, 279, 346, 457, 534
BspCNI CTCAG 1 cut(s) 709
BspHI TCATGA 2 cut(s) 493, 1138
BspLI GGNNCC 3 cut(s) 24, 253, 480
BspMI ACCTGC 2 cut(s) 254, 830
BspOI GCTAGC 1 cut(s) 965
BspPI GGATC 3 cut(s) 342, 609, 724
BspT107I GGYRCC 3 cut(s) 22, 251, 478
BspTI CTTAAG 1 cut(s) 514
BsrDI GCAATG 1 cut(s) 185
BsrFI RCCGGY 2 cut(s) 376, 487
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 2 cut(s) 458, 1204
BssAI RCCGGY 2 cut(s) 376, 487
BssECI CCNNGG 6 cut(s) 26, 256, 535, 567, 785, 903
BssMI GATC 8 cut(s) 127, 334, 510, 601, 716, 736, 1212, 1240
BssNI GRCGYC 2 cut(s) 13, 398
BssSI CACGAG 1 cut(s) 882
BssT1I CCWWGG 2 cut(s) 567, 903
Bst2BI CACGAG 1 cut(s) 882
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 4 cut(s) 275, 539, 551, 1266
Bst6I CTCTTC 2 cut(s) 35, 921
BstACI GRCGYC 2 cut(s) 13, 398
BstAFI CTTAAG 1 cut(s) 514
BstAUI TGTACA 1 cut(s) 591
BstDEI CTNAG 2 cut(s) 669, 696
BstDSI CCRYGG 1 cut(s) 535
BstEII GGTNACC 1 cut(s) 1199
BstF5I GGATG 3 cut(s) 563, 745, 1243
BstHHI GCGC 1 cut(s) 1030
BstKTI GATC 8 cut(s) 130, 337, 513, 604, 719, 739, 1215, 1243
BstMAI GTCTC 4 cut(s) 4, 41, 658, 669
BstMBI GATC 8 cut(s) 127, 334, 510, 601, 716, 736, 1212, 1240
BstMCI CGRYCG 1 cut(s) 347
BstNI CCWGG 1 cut(s) 28
BstNSI RCATGY 3 cut(s) 384, 1049, 1168
BstPI GGTNACC 1 cut(s) 1199
BstSCI CCNGG 1 cut(s) 26
BstSLI GKGCMC 2 cut(s) 27, 83
BstV1I GCAGC 5 cut(s) 447, 807, 949, 961, 1012
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
BstZI CGGCCG 1 cut(s) 344
BsuRI GGCC 5 cut(s) 261, 279, 346, 457, 534
BtgI CCRYGG 1 cut(s) 535
BtrI CACGTC 2 cut(s) 621, 679
BtsCI GGATG 3 cut(s) 563, 745, 1243
BveI ACCTGC 2 cut(s) 254, 830
CciI TCATGA 2 cut(s) 493, 1138
CfoI GCGC 1 cut(s) 1030
Cfr10I RCCGGY 2 cut(s) 376, 487
CseI GACGC 1 cut(s) 387
Csp6I GTAC 3 cut(s) 365, 592, 804
CviAII CATG 6 cut(s) 331, 381, 494, 1046, 1139, 1165
CviQI GTAC 3 cut(s) 365, 592, 804
DdeI CTNAG 2 cut(s) 669, 696
DpnI GATC 8 cut(s) 129, 336, 512, 603, 718, 738, 1214, 1242
DpnII GATC 8 cut(s) 127, 334, 510, 601, 716, 736, 1212, 1240
DraIII CACNNNGTG 1 cut(s) 22
EaeI YGGCCR 3 cut(s) 277, 344, 532
EagI CGGCCG 1 cut(s) 344
Eam1104I CTCTTC 2 cut(s) 35, 921
EarI CTCTTC 2 cut(s) 35, 921
EciI GGCGGA 1 cut(s) 923
EclXI CGGCCG 1 cut(s) 344
Eco130I CCWWGG 2 cut(s) 567, 903
Eco32I GATATC 1 cut(s) 574
Eco52I CGGCCG 1 cut(s) 344
Eco57I CTGAAG 5 cut(s) 59, 136, 450, 590, 1215
Eco88I CYCGRG 1 cut(s) 255
Eco91I GGTNACC 1 cut(s) 1199
EcoO65I GGTNACC 1 cut(s) 1199
EcoRII CCWGG 1 cut(s) 26
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 2 cut(s) 567, 903
ErhI CCWWGG 2 cut(s) 567, 903
Esp3I CGTCTC 2 cut(s) 4, 669
FaeI CATG 6 cut(s) 334, 384, 497, 1049, 1142, 1168
FaiI YATR 7 cut(s) 332, 382, 495, 645, 1047, 1140, 1166
FalI AAGNNNNNCTT 2 cut(s) 497, 529
FatI CATG 6 cut(s) 330, 380, 493, 1045, 1138, 1164
FblI GTMKAC 1 cut(s) 340
Fnu4HI GCNGC 6 cut(s) 250, 436, 796, 938, 950, 1001
FokI GGATG 3 cut(s) 550, 732, 1250
Fsp4HI GCNGC 6 cut(s) 250, 436, 796, 938, 950, 1001
FspBI CTAG 4 cut(s) 801, 962, 1116, 1221
GlaI GCGC 1 cut(s) 1029
GluI GCNGC 6 cut(s) 250, 436, 796, 938, 950, 1001
GsuI CTGGAG 1 cut(s) 686
HaeIII GGCC 5 cut(s) 261, 279, 346, 457, 534
HapII CCGG 5 cut(s) 7, 53, 347, 377, 488
HgaI GACGC 1 cut(s) 387
HhaI GCGC 1 cut(s) 1030
Hin1I GRCGYC 2 cut(s) 13, 398
Hin1II CATG 6 cut(s) 334, 384, 497, 1049, 1142, 1168
Hin6I GCGC 1 cut(s) 1028
HinP1I GCGC 1 cut(s) 1028
HindIII AAGCTT 2 cut(s) 32, 1032
HinfI GANTC 4 cut(s) 49, 350, 767, 845
HpaII CCGG 5 cut(s) 7, 53, 347, 377, 488
HphI GGTGA 6 cut(s) 70, 137, 809, 854, 1075, 1211
Hpy166II GTNNAC 1 cut(s) 341
Hpy188I TCNGA 4 cut(s) 39, 155, 223, 786
Hpy188III TCNNGA 4 cut(s) 241, 390, 494, 1139
Hpy8I GTNNAC 1 cut(s) 341
HpyAV CCTTC 3 cut(s) 150, 468, 1190
HpyCH4III ACNGT 4 cut(s) 275, 539, 551, 1266
HpyCH4IV ACGT 3 cut(s) 13, 620, 678
HpyCH4V TGCA 5 cut(s) 4, 100, 327, 581, 1098
HpyF3I CTNAG 2 cut(s) 669, 696
HpySE526I ACGT 3 cut(s) 13, 620, 678
Hsp92I GRCGYC 2 cut(s) 13, 398
Hsp92II CATG 6 cut(s) 334, 384, 497, 1049, 1142, 1168
HspAI GCGC 1 cut(s) 1028
KroI GCCGGC 1 cut(s) 487
KroNI GCCGGC 1 cut(s) 489
Kzo9I GATC 8 cut(s) 127, 334, 510, 601, 716, 736, 1212, 1240
LmnI GCTCC 2 cut(s) 705, 946
Lsp1109I GCAGC 5 cut(s) 447, 807, 949, 961, 1012
LweI GCATC 2 cut(s) 310, 860
MaeI CTAG 4 cut(s) 801, 962, 1116, 1221
MaeII ACGT 3 cut(s) 13, 620, 678
MaeIII GTNAC 5 cut(s) 14, 545, 722, 1160, 1199
MalI GATC 8 cut(s) 129, 336, 512, 603, 718, 738, 1214, 1242
MboI GATC 8 cut(s) 127, 334, 510, 601, 716, 736, 1212, 1240
MboII GAAGA 4 cut(s) 52, 596, 887, 938
MfeI CAATTG 1 cut(s) 711
MflI RGATCY 1 cut(s) 601
MhlI GDGCHC 2 cut(s) 27, 83
MlsI TGGCCA 1 cut(s) 534
MluCI AATT 8 cut(s) 427, 595, 711, 727, 867, 1016, 1106, 1249
MluNI TGGCCA 1 cut(s) 534
MlyI GAGTC 1 cut(s) 43
MnlI CCTC 9 cut(s) 54, 251, 534, 673, 780, 922, 927, 1116, 1146
Mox20I TGGCCA 1 cut(s) 534
MroNI GCCGGC 1 cut(s) 487
MscI TGGCCA 1 cut(s) 534
MseI TTAA 6 cut(s) 515, 759, 870, 893, 1053, 1178
MslI CAYNNNNRTG 2 cut(s) 318, 379
Msp20I TGGCCA 1 cut(s) 534
MspCI CTTAAG 1 cut(s) 514
MspI CCGG 5 cut(s) 7, 53, 347, 377, 488
MspR9I CCNGG 1 cut(s) 28
MunI CAATTG 1 cut(s) 711
Mva1269I GAATGC 1 cut(s) 988
MvaI CCWGG 1 cut(s) 28
NaeI GCCGGC 1 cut(s) 489
NdeII GATC 8 cut(s) 127, 334, 510, 601, 716, 736, 1212, 1240
NgoMIV GCCGGC 1 cut(s) 487
NheI GCTAGC 1 cut(s) 961
NlaIII CATG 6 cut(s) 334, 384, 497, 1049, 1142, 1168
NlaIV GGNNCC 3 cut(s) 24, 253, 480
NmeAIII GCCGAG 1 cut(s) 991
NmuCI GTSAC 4 cut(s) 14, 545, 722, 1199
NspI RCATGY 3 cut(s) 384, 1049, 1168
PaeI GCATGC 1 cut(s) 384
PagI TCATGA 2 cut(s) 493, 1138
PaqCI CACCTGC 1 cut(s) 830
PciI ACATGT 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 662
PctI GAATGC 1 cut(s) 988
PdiI GCCGGC 1 cut(s) 489
PfeI GAWTC 3 cut(s) 350, 767, 845
PflFI GACNNNGTC 1 cut(s) 619
PflMI CCANNNNNTGG 1 cut(s) 121
PkrI GCNGC 6 cut(s) 251, 437, 797, 939, 951, 1002
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
PscI ACATGT 1 cut(s) 1164
PshBI ATTAAT 1 cut(s) 759
Psp6I CCWGG 1 cut(s) 26
PspEI GGTNACC 1 cut(s) 1199
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 3 cut(s) 24, 253, 480
PsuI RGATCY 1 cut(s) 601
PsyI GACNNNGTC 1 cut(s) 619
RsaI GTAC 3 cut(s) 366, 593, 805
RsaNI GTAC 3 cut(s) 365, 592, 804
RseI CAYNNNNRTG 2 cut(s) 318, 379
SaqAI TTAA 6 cut(s) 515, 759, 870, 893, 1053, 1178
SatI GCNGC 6 cut(s) 250, 436, 796, 938, 950, 1001
Sau3AI GATC 8 cut(s) 127, 334, 510, 601, 716, 736, 1212, 1240
ScaI AGTACT 2 cut(s) 366, 805
SchI GAGTC 1 cut(s) 43
ScrFI CCNGG 1 cut(s) 28
SduI GDGCHC 2 cut(s) 27, 83
SfaNI GCATC 2 cut(s) 310, 860
SmiMI CAYNNNNRTG 2 cut(s) 318, 379
SmlI CTYRAG 3 cut(s) 239, 289, 514
SmoI CTYRAG 3 cut(s) 239, 289, 514
SpeI ACTAGT 1 cut(s) 800
SphI GCATGC 1 cut(s) 384
Sse9I AATT 8 cut(s) 427, 595, 711, 727, 867, 1016, 1106, 1249
SsiI CCGC 3 cut(s) 249, 908, 944
SspI AATATT 1 cut(s) 757
SspMI CTAG 4 cut(s) 801, 962, 1116, 1221
StyD4I CCNGG 1 cut(s) 26
StyI CCWWGG 2 cut(s) 567, 903
TaaI ACNGT 4 cut(s) 275, 539, 551, 1266
TaiI ACGT 3 cut(s) 16, 623, 681
TaqI TCGA 4 cut(s) 200, 556, 765, 1231
TasI AATT 8 cut(s) 427, 595, 711, 727, 867, 1016, 1106, 1249
TatI WGTACW 3 cut(s) 364, 591, 803
TauI GCSGC 1 cut(s) 252
TfiI GAWTC 3 cut(s) 350, 767, 845
Tru1I TTAA 6 cut(s) 515, 759, 870, 893, 1053, 1178
Tru9I TTAA 6 cut(s) 515, 759, 870, 893, 1053, 1178
TseFI GTSAC 4 cut(s) 14, 545, 722, 1199
TseI GCWGC 5 cut(s) 435, 795, 937, 949, 1000
Tsp45I GTSAC 4 cut(s) 14, 545, 722, 1199
TspDTI ATGAA 4 cut(s) 295, 825, 845, 1242
TspGWI ACGGA 2 cut(s) 402, 1250
Tth111I GACNNNGTC 1 cut(s) 619
Van91I CCANNNNNTGG 1 cut(s) 121
Vha464I CTTAAG 1 cut(s) 514
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 1 cut(s) 1249
XceI RCATGY 3 cut(s) 384, 1049, 1168
XcmI CCANNNNNNNNNTGG 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 340
XspI CTAG 4 cut(s) 801, 962, 1116, 1221
ZraI GACGTC 1 cut(s) 14
ZrmI AGTACT 2 cut(s) 366, 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.