MD14G1241600.v1.1

Protein SHOOT GRAVITROPISM 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006002
Physical Location & Seq
Reverse (-)
32014922 .. 32018286
3365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1241600.v1.1.491

Sequence Viewer

Length: 1512 bp
ATGTTACCCAATAACTCTTCTTCCTTGGTCCCTTCTTCTGATCCTTTTTCTTGCTTAGAAAATGGCAACACCAATAAAAGAAAGAGAAGACCTGCAGGCACTCCAGATCCAGATGCGGAGGTGGTGTCACTTTCGCCGAAAACCTTACTGGAATCGGATCGTTACATTTGCGAGATCTGCAACCAAGGGTTTCAGAGAGACCAGAACCTACAGATGCACCGGCGGCGGCACAAGGTGCCATGGAAGCTGCTGAAGCGAGAGACTCCGGTCGTGAAGAAGCGGGTGTTCGTGTGCCCGGAACCAAGCTGCCTGCACCATGATCCCTGCCACGCCCTCGGCGATCTCGTCGGCATCAAAAAGCATTTCAGGAGAAAGCACAGCAACCACAAACAGTGGGTTTGTGATAAGTGCTCAAAAGGGTATGCAGTTCAATCAGATTACAAGGCACATCTCAAAACCTGCGGCACCAGAGGACATTCTTGCGACTGCGGCCGTGTTTTCTCTAGGGTTGAGAGTTTCATAGAGCACCAAGACGCTTGTAACATGGATCGTGTGCGGCCGGAATCACAAACGCTGCAACCGGCAGCATGCCTGTCACGAACGGCTTCAAGTCCTAGTCCATCCTGCAGTGACAACAATTTCAGCAGGGCTCCATCCACTTGGCCTCCATCTAATAATTTGGTACTACCACATCCAAGTCCAACTGAGTACACCAAATTAATCTTAATAAACCCTCCTGATCACGCTGATCATCATCAAGCTACATTGTCCAAGAAGAATAGTAGTAATGCACATTACAATTTGGATCTTCAGCTCGCAACCACGTCGAATATTAATATTCCTATCAATGAAGTATCTGTTTCGTCTAAGAGAGAAGAAAACCATTCCACTCAGCTGCAGCTCTCAATTGGTTCATGTGAATTTGGCGGGGGTGATCGTAGTCATCAGAACAAAACTATTGAACAACATTCGAACATTATGATCGCGAATCGAAATTACTCCCCGAGGAGGAACGAGGTGAAGGAAAGGGAAAGGGAAAGCAGCAGTACTACTACTAATCATCACGAGCATGATGACGATGGTCAGAAACCTAAACCTGCCACCGTGGCTGCTGGGATATTGAGGCTGAAAGAAGAAGCGCGAGAGCAGTTAAGGCTTGCGATGGCGGAAAAGGCTTACGCCGAAGAGGCGAGGCAGCAAGCGGAGAGCCACATCAAACTGGCGGAGAAGGAGTTTGCAAATGCCAAGAGAATAAGGCAACAAGCACAGGCCGAGTTAGACAAGGCTCACGCTTTGAAAGAGCAAGCGGTCAAGAAAGTGAACTCTACCATTCTCCAAATCACTTGCCATGCCTGCAAGAAACAGTTTATGCAAGGTGCGCATGAAGCAACAACCAATCAACATTCTCTGGTTTTGAGTTACATGTCATCGGCTATAACGTCAGAAGGTGATCATCGATTAGAGAAGGATATCAGTACTCGAACCCATCATGCAAAATCTGCTAGCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C2H2

Protein Analysis

504

Amino Acids

56.51

Weight (kDa)

9.02

Isoelectric Point (pI)

66.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-2nd_BIRD-IDD PF22996 92 - 126 4.9e-18 BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD PF22995 131 - 155 4.4e-14 BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD PF22992 159 - 181 2.4e-06 BIRD-IDD transcription factor, fourth C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1380
Acc36I ACCTGC 3 cut(s) 100, 467, 1105
AccB1I GGYRCC 2 cut(s) 235, 464
AccII CGCG 2 cut(s) 986, 1141
AclWI GGATC 6 cut(s) 35, 101, 165, 314, 555, 813
AcoI YGGCCR 2 cut(s) 490, 557
AcsI RAATTY 1 cut(s) 920
AcuI CTGAAG 2 cut(s) 272, 794
AdeI CACNNNGTG 1 cut(s) 235
AfaI GTAC 4 cut(s) 684, 710, 1048, 1477
AfiI CCNNNNNNNGG 1 cut(s) 1008
AflIII ACRYGT 1 cut(s) 1422
AgsI TTSAA 4 cut(s) 431, 609, 962, 1297
AjiI CACGTC 1 cut(s) 825
AluBI AGCT 6 cut(s) 247, 306, 761, 814, 895, 901
AluI AGCT 6 cut(s) 247, 306, 761, 814, 895, 901
Alw21I GWGCWC 2 cut(s) 413, 528
Alw26I GTCTC 2 cut(s) 192, 254
AlwI GGATC 6 cut(s) 35, 101, 165, 314, 555, 813
Ama87I CYCGRG 1 cut(s) 1003
AoxI GGCC 4 cut(s) 490, 557, 662, 1269
ApeKI GCWGC 9 cut(s) 247, 306, 574, 584, 895, 898, 1041, 1109, 1195
ApoI RAATTY 1 cut(s) 920
AseI ATTAAT 2 cut(s) 719, 834
Asp700I GAANNNNTTC 1 cut(s) 604
AspLEI GCGC 3 cut(s) 1141, 1381, 1508
AspS9I GGNCC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 3 cut(s) 944, 1030, 1460
AsuII TTCGAA 1 cut(s) 971
AsuNHI GCTAGC 1 cut(s) 1502
AvaI CYCGRG 1 cut(s) 1003
AvaII GGWCC 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 296
BanI GGYRCC 2 cut(s) 235, 464
BanII GRGCYC 1 cut(s) 652
BauI CACGAG 1 cut(s) 1064
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 2 cut(s) 413, 528
BbvI GCAGC 9 cut(s) 234, 293, 561, 596, 882, 910, 1053, 1096, 1207
BccI CCATC 6 cut(s) 628, 661, 676, 1073, 1156, 1494
BceAI ACGGC 2 cut(s) 477, 618
BcgI CGANNNNNNTGC 2 cut(s) 807, 841
BclI TGATCA 3 cut(s) 739, 748, 1450
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 2 cut(s) 192, 254
BfaI CTAG 3 cut(s) 504, 615, 1503
BfmI CTRYAG 4 cut(s) 93, 209, 625, 896
BfoI RGCGCY 1 cut(s) 1509
BfuAI ACCTGC 3 cut(s) 100, 467, 1105
BglI GCCNNNNNGGC 2 cut(s) 1106, 1187
BglII AGATCT 1 cut(s) 174
BmcAI AGTACT 2 cut(s) 1048, 1477
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 1 cut(s) 28
BmeT110I CYCGRG 1 cut(s) 1003
BmgBI CACGTC 1 cut(s) 825
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 5 cut(s) 30, 237, 300, 466, 651
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 3 cut(s) 103, 204, 360
BmtI GCTAGC 1 cut(s) 1506
BoxI GACNNNNGTC 2 cut(s) 266, 1080
BpiI GAAGAC 1 cut(s) 94
BpmI CTGGAG 1 cut(s) 87
Bpu14I TTCGAA 1 cut(s) 971
BpuMI CCSGG 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 1456
BsaBI GATNNNNATC 1 cut(s) 753
BsaI GGTCTC 1 cut(s) 192
BsaJI CCNNGG 6 cut(s) 24, 184, 239, 334, 1004, 1104
BsaWI WCCGGW 1 cut(s) 265
BsaXI ACNNNNNCTCC 6 cut(s) 110, 140, 649, 679, 1217, 1247
Bsc4I CCNNNNNNNGG 1 cut(s) 1008
Bse118I RCCGGY 2 cut(s) 219, 580
Bse1I ACTGG 2 cut(s) 153, 1224
Bse8I GATNNNNATC 1 cut(s) 753
BseCI ATCGAT 1 cut(s) 1456
BseDI CCNNGG 6 cut(s) 24, 184, 239, 334, 1004, 1104
BseGI GGATG 3 cut(s) 620, 653, 691
BseJI GATNNNNATC 1 cut(s) 753
BseLI CCNNNNNNNGG 1 cut(s) 1008
BseMII CTCAG 2 cut(s) 696, 905
BseNI ACTGG 2 cut(s) 153, 1224
BseRI GAGGAG 1 cut(s) 1021
BseSI GKGCMC 1 cut(s) 296
BseX3I CGGCCG 2 cut(s) 490, 557
BseXI GCAGC 9 cut(s) 234, 293, 561, 596, 882, 910, 1053, 1096, 1207
BseYI CCCAGC 1 cut(s) 1112
BsgI GTGCAG 1 cut(s) 296
Bsh1236I CGCG 2 cut(s) 986, 1141
Bsh1285I CGRYCG 3 cut(s) 270, 493, 560
BshFI GGCC 4 cut(s) 492, 559, 664, 1271
BshNI GGYRCC 2 cut(s) 235, 464
BshVI ATCGAT 1 cut(s) 1456
BsiEI CGRYCG 3 cut(s) 270, 493, 560
BsiHKAI GWGCWC 2 cut(s) 413, 528
BsiHKCI CYCGRG 1 cut(s) 1003
BsiSI CCGG 5 cut(s) 220, 266, 296, 560, 581
BslFI GGGAC 1 cut(s) 14
BslI CCNNNNNNNGG 1 cut(s) 1008
BsmAI GTCTC 2 cut(s) 192, 254
BsmFI GGGAC 1 cut(s) 14
BsnI GGCC 4 cut(s) 492, 559, 664, 1271
Bso31I GGTCTC 1 cut(s) 192
BsoBI CYCGRG 1 cut(s) 1003
Bsp119I TTCGAA 1 cut(s) 971
Bsp1286I GDGCHC 4 cut(s) 296, 413, 528, 652
Bsp19I CCATGG 1 cut(s) 239
Bsp68I TCGCGA 1 cut(s) 986
BspANI GGCC 4 cut(s) 492, 559, 664, 1271
BspCNI CTCAG 2 cut(s) 697, 904
BspDI ATCGAT 1 cut(s) 1456
BspFNI CGCG 2 cut(s) 986, 1141
BspLI GGNNCC 5 cut(s) 30, 237, 300, 466, 651
BspMAI CTGCAG 3 cut(s) 97, 629, 900
BspMI ACCTGC 3 cut(s) 100, 467, 1105
BspOI GCTAGC 1 cut(s) 1506
BspPI GGATC 6 cut(s) 35, 101, 165, 314, 555, 813
BspT104I TTCGAA 1 cut(s) 971
BspT107I GGYRCC 2 cut(s) 235, 464
BspTNI GGTCTC 1 cut(s) 192
BsrFI RCCGGY 2 cut(s) 219, 580
BsrI ACTGG 2 cut(s) 153, 1224
BssAI RCCGGY 2 cut(s) 219, 580
BssECI CCNNGG 6 cut(s) 24, 184, 239, 334, 1004, 1104
BssSI CACGAG 1 cut(s) 1064
BssT1I CCWWGG 3 cut(s) 24, 184, 239
Bst2BI CACGAG 1 cut(s) 1064
Bst4CI ACNGT 3 cut(s) 393, 1105, 1365
Bst6I CTCTTC 2 cut(s) 22, 1179
BstAPI GCANNNNNTGC 2 cut(s) 235, 1499
BstBI TTCGAA 1 cut(s) 971
BstC8I GCNNGC 9 cut(s) 97, 311, 589, 816, 1158, 1200, 1305, 1354, 1504
BstDEI CTNAG 4 cut(s) 55, 705, 867, 891
BstDSI CCRYGG 2 cut(s) 239, 1104
BstF5I GGATG 3 cut(s) 620, 653, 691
BstFNI CGCG 2 cut(s) 986, 1141
BstH2I RGCGCY 1 cut(s) 1509
BstHHI GCGC 3 cut(s) 1141, 1381, 1508
BstMAI GTCTC 2 cut(s) 192, 254
BstMCI CGRYCG 3 cut(s) 270, 493, 560
BstNSI RCATGY 2 cut(s) 591, 1426
BstPAI GACNNNNGTC 2 cut(s) 266, 1080
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 4 cut(s) 93, 209, 625, 896
BstSLI GKGCMC 1 cut(s) 296
BstUI CGCG 2 cut(s) 986, 1141
BstV1I GCAGC 9 cut(s) 234, 293, 561, 596, 882, 910, 1053, 1096, 1207
BstV2I GAAGAC 1 cut(s) 94
BstX2I RGATCY 3 cut(s) 106, 174, 805
BstXI CCANNNNNNTGG 1 cut(s) 660
BstYI RGATCY 3 cut(s) 106, 174, 805
BstZI CGGCCG 2 cut(s) 490, 557
Bsu15I ATCGAT 1 cut(s) 1456
BsuRI GGCC 4 cut(s) 492, 559, 664, 1271
BsuTUI ATCGAT 1 cut(s) 1456
BtgI CCRYGG 2 cut(s) 239, 1104
BtgZI GCGATG 1 cut(s) 1175
BtrI CACGTC 1 cut(s) 825
BtsCI GGATG 3 cut(s) 620, 653, 691
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 2 cut(s) 398, 634
BtuMI TCGCGA 1 cut(s) 986
BveI ACCTGC 3 cut(s) 100, 467, 1105
Cac8I GCNNGC 9 cut(s) 97, 311, 589, 816, 1158, 1200, 1305, 1354, 1504
CfoI GCGC 3 cut(s) 1141, 1381, 1508
Cfr10I RCCGGY 2 cut(s) 219, 580
Cfr13I GGNCC 1 cut(s) 28
ClaI ATCGAT 1 cut(s) 1456
CseI GACGC 1 cut(s) 542
Csp6I GTAC 4 cut(s) 683, 709, 1047, 1476
CviQI GTAC 4 cut(s) 683, 709, 1047, 1476
DdeI CTNAG 4 cut(s) 55, 705, 867, 891
DraIII CACNNNGTG 1 cut(s) 235
EaeI YGGCCR 2 cut(s) 490, 557
EagI CGGCCG 2 cut(s) 490, 557
Eam1104I CTCTTC 2 cut(s) 22, 1179
EarI CTCTTC 2 cut(s) 22, 1179
EciI GGCGGA 2 cut(s) 1181, 1238
EclXI CGGCCG 2 cut(s) 490, 557
Eco130I CCWWGG 3 cut(s) 24, 184, 239
Eco24I GRGCYC 1 cut(s) 652
Eco31I GGTCTC 1 cut(s) 192
Eco32I GATATC 1 cut(s) 1471
Eco47I GGWCC 1 cut(s) 28
Eco52I CGGCCG 2 cut(s) 490, 557
Eco57I CTGAAG 2 cut(s) 272, 794
Eco88I CYCGRG 1 cut(s) 1003
EcoRV GATATC 1 cut(s) 1471
EcoT14I CCWWGG 3 cut(s) 24, 184, 239
EcoT38I GRGCYC 1 cut(s) 652
ErhI CCWWGG 3 cut(s) 24, 184, 239
FaqI GGGAC 1 cut(s) 14
FauI CCCGC 2 cut(s) 273, 920
FbaI TGATCA 3 cut(s) 739, 748, 1450
FokI GGATG 3 cut(s) 607, 640, 678
FriOI GRGCYC 1 cut(s) 652
FspAI RTGCGCAY 1 cut(s) 1380
FspBI CTAG 3 cut(s) 504, 615, 1503
FspI TGCGCA 1 cut(s) 1380
GlaI GCGC 3 cut(s) 1140, 1380, 1507
GsaI CCCAGC 1 cut(s) 1116
GsuI CTGGAG 1 cut(s) 87
HaeII RGCGCY 1 cut(s) 1509
HaeIII GGCC 4 cut(s) 492, 559, 664, 1271
HapII CCGG 5 cut(s) 220, 266, 296, 560, 581
HgaI GACGC 1 cut(s) 542
HhaI GCGC 3 cut(s) 1141, 1381, 1508
Hin6I GCGC 3 cut(s) 1139, 1379, 1506
HinP1I GCGC 3 cut(s) 1139, 1379, 1506
HinfI GANTC 4 cut(s) 152, 262, 563, 988
HpaII CCGG 5 cut(s) 220, 266, 296, 560, 581
HphI GGTGA 3 cut(s) 944, 1030, 1460
Hpy166II GTNNAC 2 cut(s) 711, 1321
Hpy188I TCNGA 7 cut(s) 40, 157, 195, 436, 948, 1086, 1444
Hpy188III TCNNGA 9 cut(s) 104, 110, 271, 367, 597, 737, 985, 1064, 1312
Hpy8I GTNNAC 2 cut(s) 711, 1321
Hpy99I CGWCG 2 cut(s) 350, 829
HpyAV CCTTC 5 cut(s) 42, 1015, 1222, 1439, 1459
HpyCH4III ACNGT 3 cut(s) 393, 1105, 1365
HpyCH4IV ACGT 2 cut(s) 824, 1439
HpyF3I CTNAG 4 cut(s) 55, 705, 867, 891
HpySE526I ACGT 2 cut(s) 824, 1439
HspAI GCGC 3 cut(s) 1139, 1379, 1506
Ksp22I TGATCA 3 cut(s) 739, 748, 1450
LmnI GCTCC 1 cut(s) 655
Lsp1109I GCAGC 9 cut(s) 234, 293, 561, 596, 882, 910, 1053, 1096, 1207
LweI GCATC 3 cut(s) 103, 204, 360
MaeI CTAG 3 cut(s) 504, 615, 1503
MaeII ACGT 2 cut(s) 824, 1439
MaeIII GTNAC 7 cut(s) 3, 126, 161, 539, 594, 629, 1418
MfeI CAATTG 1 cut(s) 906
MflI RGATCY 3 cut(s) 106, 174, 805
MhlI GDGCHC 4 cut(s) 296, 413, 528, 652
MluCI AATT 7 cut(s) 637, 676, 716, 799, 906, 920, 994
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 1 cut(s) 725
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 4 cut(s) 719, 725, 834, 1151
MslI CAYNNNNRTG 1 cut(s) 1068
MspA1I CMGCKG 1 cut(s) 895
MspI CCGG 5 cut(s) 220, 266, 296, 560, 581
MspR9I CCNGG 1 cut(s) 296
MunI CAATTG 1 cut(s) 906
MvnI CGCG 2 cut(s) 986, 1141
NciI CCSGG 1 cut(s) 296
NcoI CCATGG 1 cut(s) 239
NheI GCTAGC 1 cut(s) 1502
NlaIV GGNNCC 5 cut(s) 30, 237, 300, 466, 651
NmeAIII GCCGAG 2 cut(s) 315, 1297
NmuCI GTSAC 3 cut(s) 126, 594, 629
NruI TCGCGA 1 cut(s) 986
NsbI TGCGCA 1 cut(s) 1380
NspI RCATGY 2 cut(s) 591, 1426
NspV TTCGAA 1 cut(s) 971
PaeI GCATGC 1 cut(s) 591
PciI ACATGT 1 cut(s) 1422
PcsI WCGNNNNNNNCGW 3 cut(s) 336, 342, 1436
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 3 cut(s) 152, 563, 988
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
PscI ACATGT 1 cut(s) 1422
PshAI GACNNNNGTC 2 cut(s) 266, 1080
PshBI ATTAAT 2 cut(s) 719, 834
PspFI CCCAGC 1 cut(s) 1112
PspN4I GGNNCC 5 cut(s) 30, 237, 300, 466, 651
PspPI GGNCC 1 cut(s) 28
PstI CTGCAG 3 cut(s) 97, 629, 900
PsuI RGATCY 3 cut(s) 106, 174, 805
PvuII CAGCTG 1 cut(s) 895
RruI TCGCGA 1 cut(s) 986
RsaI GTAC 4 cut(s) 684, 710, 1048, 1477
RsaNI GTAC 4 cut(s) 683, 709, 1047, 1476
RseI CAYNNNNRTG 1 cut(s) 1068
SaqAI TTAA 4 cut(s) 719, 725, 834, 1151
Sau96I GGNCC 1 cut(s) 28
SbfI CCTGCAGG 1 cut(s) 97
ScaI AGTACT 2 cut(s) 1048, 1477
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 1 cut(s) 296
SdaI CCTGCAGG 1 cut(s) 97
SduI GDGCHC 4 cut(s) 296, 413, 528, 652
SfaNI GCATC 3 cut(s) 103, 204, 360
SfcI CTRYAG 4 cut(s) 93, 209, 625, 896
SfuI TTCGAA 1 cut(s) 971
SgrAI CRCCGGYG 1 cut(s) 219
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 1068
SphI GCATGC 1 cut(s) 591
Sse8387I CCTGCAGG 1 cut(s) 97
Sse9I AATT 7 cut(s) 637, 676, 716, 799, 906, 920, 994
SspI AATATT 2 cut(s) 832, 838
SspMI CTAG 3 cut(s) 504, 615, 1503
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 3 cut(s) 24, 184, 239
TaaI ACNGT 3 cut(s) 393, 1105, 1365
TaiI ACGT 2 cut(s) 827, 1442
TaqI TCGA 5 cut(s) 827, 971, 991, 1456, 1480
TasI AATT 7 cut(s) 637, 676, 716, 799, 906, 920, 994
TatI WGTACW 3 cut(s) 708, 1046, 1475
TauI GCSGC 5 cut(s) 226, 229, 465, 492, 559
TfiI GAWTC 3 cut(s) 152, 563, 988
Tru1I TTAA 4 cut(s) 719, 725, 834, 1151
Tru9I TTAA 4 cut(s) 719, 725, 834, 1151
TscAI CASTG 2 cut(s) 398, 634
TseFI GTSAC 3 cut(s) 126, 594, 629
TseI GCWGC 9 cut(s) 247, 306, 574, 584, 895, 898, 1041, 1109, 1195
Tsp45I GTSAC 3 cut(s) 126, 594, 629
TspDTI ATGAA 4 cut(s) 508, 864, 903, 1398
TspRI CASTG 2 cut(s) 398, 634
VpaK11BI GGWCC 1 cut(s) 28
VspI ATTAAT 2 cut(s) 719, 834
XapI RAATTY 1 cut(s) 920
XceI RCATGY 2 cut(s) 591, 1426
XmnI GAANNNNTTC 1 cut(s) 604
XspI CTAG 3 cut(s) 504, 615, 1503
ZrmI AGTACT 2 cut(s) 1048, 1477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.